Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0974440 | XP_013457864.1 | 77.838 | 185 | 40 | 1 | 3 | 186 | 173 | 357 | 7.71e-82 | 275 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0974440 | sp|F4HVS0|BTSL2_ARATH | 32.447 | 188 | 118 | 4 | 3 | 186 | 165 | 347 | 3.86e-16 | 79.0 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0974440 | A0A072V1D4 | 77.838 | 185 | 40 | 1 | 3 | 186 | 173 | 357 | 3.68e-82 | 275 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0701010 | Msa0974440 | 0.814054 | 1.872806e-51 | -8.615850e-47 |
| Msa0735660 | Msa0974440 | 0.804266 | 2.345846e-49 | -8.615850e-47 |
| Msa0424320 | Msa0974440 | 0.808202 | 3.478186e-50 | -8.615850e-47 |
| Msa0311750 | Msa0974440 | 0.818504 | 1.893637e-52 | -8.615850e-47 |
| Msa0877250 | Msa0974440 | 0.842962 | 1.876514e-58 | -8.615850e-47 |
| Msa0899430 | Msa0974440 | 0.804802 | 1.813821e-49 | -8.615850e-47 |
| Msa0958550 | Msa0974440 | 0.802570 | 5.269818e-49 | -8.615850e-47 |
| Msa0966750 | Msa0974440 | 0.805404 | 1.357065e-49 | -8.615850e-47 |
| Msa0974440 | Msa1107620 | 0.824305 | 8.676310e-54 | -8.615850e-47 |
| Msa0974440 | Msa1184420 | 0.803112 | 4.071756e-49 | -8.615850e-47 |
| Msa0974440 | Msa1321080 | 0.833378 | 5.520025e-56 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0974440 | MtrunA17_Chr4g0059881 | 77.838 | 185 | 40 | 1 | 3 | 186 | 173 | 357 | 7.09e-86 | 275 |
| Msa0974440 | MtrunA17_Chr6g0484171 | 31.472 | 197 | 109 | 6 | 3 | 186 | 171 | 354 | 2.81e-18 | 82.0 |
| Msa0974440 | MtrunA17_Chr8g0391161 | 29.500 | 200 | 116 | 6 | 3 | 186 | 166 | 356 | 1.93e-16 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0974440 | AT1G74770.1 | 32.447 | 188 | 118 | 4 | 3 | 186 | 165 | 347 | 3.93e-17 | 79.0 |
| Msa0974440 | AT1G74770.2 | 32.447 | 188 | 118 | 4 | 3 | 186 | 165 | 347 | 4.48e-17 | 79.0 |
| Msa0974440 | AT3G18290.1 | 28.866 | 194 | 120 | 6 | 3 | 186 | 180 | 365 | 5.47e-16 | 75.9 |
| Msa0974440 | AT1G18910.1 | 31.383 | 188 | 121 | 3 | 3 | 186 | 173 | 356 | 1.49e-15 | 74.3 |
Find 31 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTAATCCAACTAAAATTCT+TGG | 0.275137 | 6_4:-7465757 | Msa0974440:CDS |
| ACTTCCTCCAGCAACATTAT+AGG | 0.279336 | 6_4:+7466291 | None:intergenic |
| TGTCTGACCTGAAATTTCTT+TGG | 0.284002 | 6_4:+7465935 | None:intergenic |
| AGGAACTCCAAAGAAATTTC+AGG | 0.336881 | 6_4:-7465942 | Msa0974440:CDS |
| CTAATAAGAAATTCCGGTTC+CGG | 0.342631 | 6_4:-7465804 | Msa0974440:CDS |
| TTGGTCCGAGTTCATTCAAT+AGG | 0.354396 | 6_4:-7465962 | Msa0974440:CDS |
| CTTTATCTAATAAGAAATTC+CGG | 0.393478 | 6_4:-7465810 | Msa0974440:CDS |
| AGATTGGGTCAACCAGGTTA+AGG | 0.419092 | 6_4:-7465889 | Msa0974440:CDS |
| AGCAACAAACAAACCTTCAC+TGG | 0.431741 | 6_4:-7466047 | Msa0974440:CDS |
| TAGTGTTCCTATAATGTTGC+TGG | 0.432236 | 6_4:-7466298 | Msa0974440:CDS |
| TCTTGCAGTGCTGTTTCCAT+TGG | 0.434812 | 6_4:+7466205 | None:intergenic |
| TGATAGCGTTATGCCAAAGA+TGG | 0.442730 | 6_4:+7465862 | None:intergenic |
| TAACAAAGAAACAGAAGATT+GGG | 0.442902 | 6_4:-7465904 | Msa0974440:CDS |
| CTGCTTGTTTATCTGCTGAC+AGG | 0.474479 | 6_4:+7466257 | None:intergenic |
| TTTCTATATGCAAGAATCCA+TGG | 0.478233 | 6_4:+7465991 | None:intergenic |
| ATAACAAAGAAACAGAAGAT+TGG | 0.489453 | 6_4:-7465905 | Msa0974440:CDS |
| TTGATGTCCTCATCTTCTAC+AGG | 0.495710 | 6_4:-7465734 | Msa0974440:CDS |
| TTTCAGAGTGAGGAAATTCA+AGG | 0.502671 | 6_4:-7466017 | Msa0974440:CDS |
| AACAGAAGATTGGGTCAACC+AGG | 0.542861 | 6_4:-7465895 | Msa0974440:CDS |
| GAGGAAATTCAAGGTTGCCA+TGG | 0.554452 | 6_4:-7466008 | Msa0974440:CDS |
| GAGTTCCTATTGAATGAACT+CGG | 0.571210 | 6_4:+7465957 | None:intergenic |
| TCAAATTTATCCACTGCCGC+AGG | 0.573609 | 6_4:-7466388 | None:intergenic |
| TTGTTTATCTGCTGACAGGA+AGG | 0.573644 | 6_4:+7466261 | None:intergenic |
| AATATTACCTGTAGAAGATG+AGG | 0.589898 | 6_4:+7465727 | None:intergenic |
| CTGAAAATATGTCCCAGTGA+AGG | 0.605019 | 6_4:+7466034 | None:intergenic |
| AAGAGCAAGCCTCACTTGTG+TGG | 0.630967 | 6_4:-7466332 | Msa0974440:CDS |
| AATGGAAACAGCACTGCAAG+AGG | 0.632913 | 6_4:-7466203 | Msa0974440:CDS |
| AAAGATGGAAAACCTTAACC+TGG | 0.640290 | 6_4:+7465877 | None:intergenic |
| GCAACAAACAAACCTTCACT+GGG | 0.643067 | 6_4:-7466046 | Msa0974440:CDS |
| TATGAATTGCCACACAAGTG+AGG | 0.645881 | 6_4:+7466323 | None:intergenic |
| TGTTCCTATAATGTTGCTGG+AGG | 0.651755 | 6_4:-7466295 | Msa0974440:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAGATTTGAATTATTAAAA+AGG | + | chr6_4:7466010-7466029 | None:intergenic | 10.0% |
| ! | GGGGGGGGGGGGGGGGGGGG+GGG | + | chr6_4:7465968-7465987 | None:intergenic | 100.0% |
| !! | ATTTGAATTATTAAAAAGGT+GGG | + | chr6_4:7466006-7466025 | None:intergenic | 15.0% |
| !! | GATTTGAATTATTAAAAAGG+TGG | + | chr6_4:7466007-7466026 | None:intergenic | 20.0% |
| !! | TTGCATATAGAAAAATCATT+TGG | - | chr6_4:7466125-7466144 | Msa0974440:intron | 20.0% |
| !! | CTTTATCTAATAAGAAATTC+CGG | - | chr6_4:7466296-7466315 | Msa0974440:CDS | 20.0% |
| !! | TTTTAATGAAATTGCACCAA+TGG | - | chr6_4:7465885-7465904 | Msa0974440:CDS | 25.0% |
| !! | AAATCTAATTGCGAAGATTT+TGG | - | chr6_4:7466022-7466041 | Msa0974440:CDS | 25.0% |
| ! | ATAACAAAGAAACAGAAGAT+TGG | - | chr6_4:7466201-7466220 | Msa0974440:CDS | 25.0% |
| ! | TAACAAAGAAACAGAAGATT+GGG | - | chr6_4:7466202-7466221 | Msa0974440:CDS | 25.0% |
| ! | ACTAATCCAACTAAAATTCT+TGG | - | chr6_4:7466349-7466368 | Msa0974440:CDS | 25.0% |
| ! | GCTCTTTAGTAGACAATTTT+TGG | + | chr6_4:7465760-7465779 | None:intergenic | 30.0% |
| TTTCTATATGCAAGAATCCA+TGG | + | chr6_4:7466118-7466137 | None:intergenic | 30.0% | |
| !! | CATCAACCAAGAATTTTAGT+TGG | + | chr6_4:7466358-7466377 | None:intergenic | 30.0% |
| !! | GACAATTTTTGGATCAGAAG+AGG | + | chr6_4:7465749-7465768 | None:intergenic | 35.0% |
| TAGTGTTCCTATAATGTTGC+TGG | - | chr6_4:7465808-7465827 | Msa0974440:CDS | 35.0% | |
| !!! | TTGCGAAGATTTTGGTTTCT+TGG | - | chr6_4:7466030-7466049 | Msa0974440:CDS | 35.0% |
| !!! | AAGATTTTGGTTTCTTGGCT+TGG | - | chr6_4:7466035-7466054 | Msa0974440:CDS | 35.0% |
| TTTCAGAGTGAGGAAATTCA+AGG | - | chr6_4:7466089-7466108 | Msa0974440:intron | 35.0% | |
| GAGTTCCTATTGAATGAACT+CGG | + | chr6_4:7466152-7466171 | None:intergenic | 35.0% | |
| AGGAACTCCAAAGAAATTTC+AGG | - | chr6_4:7466164-7466183 | Msa0974440:intron | 35.0% | |
| TGTCTGACCTGAAATTTCTT+TGG | + | chr6_4:7466174-7466193 | None:intergenic | 35.0% | |
| AAAGATGGAAAACCTTAACC+TGG | + | chr6_4:7466232-7466251 | None:intergenic | 35.0% | |
| ! | AGGTTAAGGTTTTCCATCTT+TGG | - | chr6_4:7466231-7466250 | Msa0974440:CDS | 35.0% |
| CTAATAAGAAATTCCGGTTC+CGG | - | chr6_4:7466302-7466321 | Msa0974440:CDS | 35.0% | |
| !! | GTCTAAATTTTGAGAACAGC+CGG | + | chr6_4:7466324-7466343 | None:intergenic | 35.0% |
| ATCAGAAGAGGAAAAACCTG+CGG | + | chr6_4:7465737-7465756 | None:intergenic | 40.0% | |
| TATGAATTGCCACACAAGTG+AGG | + | chr6_4:7465786-7465805 | None:intergenic | 40.0% | |
| TGTTCCTATAATGTTGCTGG+AGG | - | chr6_4:7465811-7465830 | Msa0974440:CDS | 40.0% | |
| ACTTCCTCCAGCAACATTAT+AGG | + | chr6_4:7465818-7465837 | None:intergenic | 40.0% | |
| TTGTTTATCTGCTGACAGGA+AGG | + | chr6_4:7465848-7465867 | None:intergenic | 40.0% | |
| AGCAACAAACAAACCTTCAC+TGG | - | chr6_4:7466059-7466078 | Msa0974440:CDS | 40.0% | |
| GCAACAAACAAACCTTCACT+GGG | - | chr6_4:7466060-7466079 | Msa0974440:CDS | 40.0% | |
| CTGAAAATATGTCCCAGTGA+AGG | + | chr6_4:7466075-7466094 | None:intergenic | 40.0% | |
| ! | TGGGACATATTTTCAGAGTG+AGG | - | chr6_4:7466079-7466098 | Msa0974440:intron | 40.0% |
| TTGGTCCGAGTTCATTCAAT+AGG | - | chr6_4:7466144-7466163 | Msa0974440:intron | 40.0% | |
| ! | TGATAGCGTTATGCCAAAGA+TGG | + | chr6_4:7466247-7466266 | None:intergenic | 40.0% |
| TTGATGTCCTCATCTTCTAC+AGG | - | chr6_4:7466372-7466391 | Msa0974440:CDS | 40.0% | |
| CTGCTTGTTTATCTGCTGAC+AGG | + | chr6_4:7465852-7465871 | None:intergenic | 45.0% | |
| !! | TCTTGCAGTGCTGTTTCCAT+TGG | + | chr6_4:7465904-7465923 | None:intergenic | 45.0% |
| AATGGAAACAGCACTGCAAG+AGG | - | chr6_4:7465903-7465922 | Msa0974440:CDS | 45.0% | |
| TAAAAAGGTGGGTGTGTGTG+TGG | + | chr6_4:7465995-7466014 | None:intergenic | 45.0% | |
| GAGGAAATTCAAGGTTGCCA+TGG | - | chr6_4:7466098-7466117 | Msa0974440:intron | 45.0% | |
| AACAGAAGATTGGGTCAACC+AGG | - | chr6_4:7466211-7466230 | Msa0974440:CDS | 45.0% | |
| AGATTGGGTCAACCAGGTTA+AGG | - | chr6_4:7466217-7466236 | Msa0974440:CDS | 45.0% | |
| !! | ATTTTGAGAACAGCCGGAAC+CGG | + | chr6_4:7466318-7466337 | None:intergenic | 45.0% |
| AAGAGCAAGCCTCACTTGTG+TGG | - | chr6_4:7465774-7465793 | Msa0974440:CDS | 50.0% | |
| AAGGTGGGTGTGTGTGTGGT+GGG | + | chr6_4:7465991-7466010 | None:intergenic | 55.0% | |
| AAAGGTGGGTGTGTGTGTGG+TGG | + | chr6_4:7465992-7466011 | None:intergenic | 55.0% | |
| AGGTGGGTGTGTGTGTGGTG+GGG | + | chr6_4:7465990-7466009 | None:intergenic | 60.0% | |
| ! | TGGGTGTGTGTGTGGTGGGG+GGG | + | chr6_4:7465987-7466006 | None:intergenic | 65.0% |
| ! | GTGGGTGTGTGTGTGGTGGG+GGG | + | chr6_4:7465988-7466007 | None:intergenic | 65.0% |
| GGTGGGTGTGTGTGTGGTGG+GGG | + | chr6_4:7465989-7466008 | None:intergenic | 65.0% | |
| ! | TGTGTGTGTGGTGGGGGGGG+GGG | + | chr6_4:7465983-7466002 | None:intergenic | 70.0% |
| ! | GTGTGTGTGTGGTGGGGGGG+GGG | + | chr6_4:7465984-7466003 | None:intergenic | 70.0% |
| ! | GGTGTGTGTGTGGTGGGGGG+GGG | + | chr6_4:7465985-7466004 | None:intergenic | 70.0% |
| ! | GGGTGTGTGTGTGGTGGGGG+GGG | + | chr6_4:7465986-7466005 | None:intergenic | 70.0% |
| ! | TGTGTGTGGTGGGGGGGGGG+GGG | + | chr6_4:7465981-7466000 | None:intergenic | 75.0% |
| ! | GTGTGTGTGGTGGGGGGGGG+GGG | + | chr6_4:7465982-7466001 | None:intergenic | 75.0% |
| !! | TGTGTGGTGGGGGGGGGGGG+GGG | + | chr6_4:7465979-7465998 | None:intergenic | 80.0% |
| !! | GTGTGTGGTGGGGGGGGGGG+GGG | + | chr6_4:7465980-7465999 | None:intergenic | 80.0% |
| !! | TGTGGTGGGGGGGGGGGGGG+GGG | + | chr6_4:7465977-7465996 | None:intergenic | 85.0% |
| !! | GTGTGGTGGGGGGGGGGGGG+GGG | + | chr6_4:7465978-7465997 | None:intergenic | 85.0% |
| ! | TGGTGGGGGGGGGGGGGGGG+GGG | + | chr6_4:7465975-7465994 | None:intergenic | 90.0% |
| !! | GTGGTGGGGGGGGGGGGGGG+GGG | + | chr6_4:7465976-7465995 | None:intergenic | 90.0% |
| ! | TGGGGGGGGGGGGGGGGGGG+GGG | + | chr6_4:7465972-7465991 | None:intergenic | 95.0% |
| ! | GTGGGGGGGGGGGGGGGGGG+GGG | + | chr6_4:7465973-7465992 | None:intergenic | 95.0% |
| ! | GGTGGGGGGGGGGGGGGGGG+GGG | + | chr6_4:7465974-7465993 | None:intergenic | 95.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6_4 | gene | 7465731 | 7466397 | 7465731 | ID=Msa0974440;Name=Msa0974440 |
| chr6_4 | mRNA | 7465731 | 7466397 | 7465731 | ID=Msa0974440-mRNA-1;Parent=Msa0974440;Name=Msa0974440-mRNA-1;_AED=0.24;_eAED=0.24;_QI=0|0|0|1|1|1|2|0|187 |
| chr6_4 | exon | 7466194 | 7466397 | 7466194 | ID=Msa0974440-mRNA-1:exon:2904;Parent=Msa0974440-mRNA-1 |
| chr6_4 | exon | 7465731 | 7466090 | 7465731 | ID=Msa0974440-mRNA-1:exon:2903;Parent=Msa0974440-mRNA-1 |
| chr6_4 | CDS | 7466194 | 7466397 | 7466194 | ID=Msa0974440-mRNA-1:cds;Parent=Msa0974440-mRNA-1 |
| chr6_4 | CDS | 7465731 | 7466090 | 7465731 | ID=Msa0974440-mRNA-1:cds;Parent=Msa0974440-mRNA-1 |
| Gene Sequence |
| Protein sequence |
