Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0311750 | XP_013457864.1 | 100.000 | 35 | 0 | 0 | 17 | 51 | 173 | 207 | 1.41e-11 | 71.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0142220 | Msa0311750 | 0.833329 | 5.676214e-56 | -8.615850e-47 |
| Msa0232850 | Msa0311750 | 0.800658 | 1.299580e-48 | -8.615850e-47 |
| Msa0251320 | Msa0311750 | 0.810527 | 1.102495e-50 | -8.615850e-47 |
| Msa0311750 | Msa0349360 | 0.800218 | 1.597716e-48 | -8.615850e-47 |
| Msa0311750 | Msa0360510 | 0.809016 | 2.330261e-50 | -8.615850e-47 |
| Msa0311750 | Msa0384800 | 0.835892 | 1.287609e-56 | -8.615850e-47 |
| Msa0311750 | Msa0390070 | 0.802313 | 5.953327e-49 | -8.615850e-47 |
| Msa0311750 | Msa0428000 | 0.811446 | 6.972711e-51 | -8.615850e-47 |
| Msa0311750 | Msa0435230 | 0.816566 | 5.177575e-52 | -8.615850e-47 |
| Msa0311750 | Msa0436780 | 0.819521 | 1.111986e-52 | -8.615850e-47 |
| Msa0311750 | Msa0436800 | 0.823364 | 1.441707e-53 | -8.615850e-47 |
| Msa0311750 | Msa0452900 | 0.808919 | 2.444913e-50 | -8.615850e-47 |
| Msa0311750 | Msa0453120 | 0.826368 | 2.819609e-54 | -8.615850e-47 |
| Msa0311750 | Msa0479340 | 0.826829 | 2.188213e-54 | -8.615850e-47 |
| Msa0311750 | Msa0506270 | 0.822039 | 2.931761e-53 | -8.615850e-47 |
| Msa0311750 | Msa0639610 | 0.813783 | 2.148546e-51 | -8.615850e-47 |
| Msa0311750 | Msa0694370 | 0.810482 | 1.127730e-50 | -8.615850e-47 |
| Msa0311750 | Msa0726140 | 0.826462 | 2.677928e-54 | -8.615850e-47 |
| Msa0311750 | Msa0743810 | 0.805047 | 1.611641e-49 | -8.615850e-47 |
| Msa0311750 | Msa0745060 | 0.802264 | 6.091531e-49 | -8.615850e-47 |
| Msa0311750 | Msa0783160 | 0.803811 | 2.916864e-49 | -8.615850e-47 |
| Msa0311750 | Msa0784290 | 0.802271 | 6.073136e-49 | -8.615850e-47 |
| Msa0311750 | Msa0824600 | 0.803336 | 3.659782e-49 | -8.615850e-47 |
| Msa0311750 | Msa0846330 | 0.802282 | 6.040608e-49 | -8.615850e-47 |
| Msa0311750 | Msa0846350 | 0.813312 | 2.727853e-51 | -8.615850e-47 |
| Msa0311750 | Msa0858980 | 0.820249 | 7.580293e-53 | -8.615850e-47 |
| Msa0311750 | Msa0877250 | 0.814083 | 1.845396e-51 | -8.615850e-47 |
| Msa0311750 | Msa0907260 | 0.825479 | 4.583983e-54 | -8.615850e-47 |
| Msa0311750 | Msa0956270 | 0.823160 | 1.608751e-53 | -8.615850e-47 |
| Msa0311750 | Msa0957860 | 0.805979 | 1.027570e-49 | -8.615850e-47 |
| Msa0311750 | Msa0957960 | 0.807313 | 5.372142e-50 | -8.615850e-47 |
| Msa0311750 | Msa0974440 | 0.818504 | 1.893637e-52 | -8.615850e-47 |
| Msa0311750 | Msa0989060 | 0.820418 | 6.930531e-53 | -8.615850e-47 |
| Msa0311750 | Msa1016280 | 0.805108 | 1.565032e-49 | -8.615850e-47 |
| Msa0311750 | Msa1025360 | 0.803213 | 3.880360e-49 | -8.615850e-47 |
| Msa0311750 | Msa1044830 | 0.802044 | 6.761824e-49 | -8.615850e-47 |
| Msa0311750 | Msa1044840 | 0.804300 | 2.308137e-49 | -8.615850e-47 |
| Msa0311750 | Msa1092990 | 0.817813 | 2.713916e-52 | -8.615850e-47 |
| Msa0311750 | Msa1099970 | 0.800359 | 1.495595e-48 | -8.615850e-47 |
| Msa0311750 | Msa1246880 | 0.807664 | 4.524998e-50 | -8.615850e-47 |
| Msa0311750 | Msa1248310 | 0.814847 | 1.250668e-51 | -8.615850e-47 |
| Msa0311750 | Msa1248370 | 0.805954 | 1.040318e-49 | -8.615850e-47 |
| Msa0311750 | Msa1269670 | 0.805670 | 1.193385e-49 | -8.615850e-47 |
| Msa0311750 | Msa1270230 | 0.805799 | 1.121398e-49 | -8.615850e-47 |
| Msa0311750 | Msa1283330 | 0.808934 | 2.426023e-50 | -8.615850e-47 |
| Msa0311750 | Msa1285930 | 0.811280 | 7.576299e-51 | -8.615850e-47 |
| Msa0311750 | Msa1289430 | 0.808649 | 2.792391e-50 | -8.615850e-47 |
| Msa0311750 | Msa1321080 | 0.887882 | 9.654603e-73 | -8.615850e-47 |
| Msa0311750 | Msa1369430 | 0.812492 | 4.126703e-51 | -8.615850e-47 |
| Msa0311750 | Msa1386330 | 0.829161 | 6.010549e-55 | -8.615850e-47 |
| Msa0311750 | Msa1402790 | 0.803760 | 2.989035e-49 | -8.615850e-47 |
| Msa0311750 | Msa1404000 | 0.836408 | 9.525258e-57 | -8.615850e-47 |
| Msa0311750 | Msa1422180 | 0.818261 | 2.149542e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0311750 | MtrunA17_Chr4g0059881 | 100.000 | 35 | 0 | 0 | 17 | 51 | 173 | 207 | 1.29e-15 | 71.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0311750 | AT1G74770.1 | 77.143 | 35 | 8 | 0 | 17 | 51 | 165 | 199 | 1.71e-11 | 59.7 |
| Msa0311750 | AT1G74770.2 | 77.143 | 35 | 8 | 0 | 17 | 51 | 165 | 199 | 1.86e-11 | 59.7 |
| Msa0311750 | AT1G18910.1 | 71.429 | 35 | 10 | 0 | 17 | 51 | 173 | 207 | 7.19e-11 | 58.2 |
Find 18 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTCCTCCAGCAACATTAT+AGG | 0.279336 | 2_4:-6633817 | None:intergenic |
| TTGGTCCGAGTTCATTCAAT+AGG | 0.354396 | 2_4:+6634148 | Msa0311750:three_prime_UTR |
| AGCAACAAACAAACCTTCAC+TGG | 0.431741 | 2_4:+6634063 | Msa0311750:CDS |
| TAGTGTTCCTATAATGTTGC+TGG | 0.432236 | 2_4:+6633810 | Msa0311750:CDS |
| TCTTGCAGTGCTGTTTCCAT+TGG | 0.434812 | 2_4:-6633903 | None:intergenic |
| CTGCTTGTTTATCTGCTGAC+AGG | 0.474479 | 2_4:-6633851 | None:intergenic |
| TTTCTATATGCAAGAATCCA+TGG | 0.478233 | 2_4:-6634119 | None:intergenic |
| TTTCAGAGTGAGGAAATTCA+AGG | 0.502671 | 2_4:+6634093 | Msa0311750:CDS |
| GAGGAAATTCAAGGTTGCCA+TGG | 0.554452 | 2_4:+6634102 | Msa0311750:CDS |
| GAGTTCCTATTGAATGAACT+CGG | 0.571210 | 2_4:-6634153 | None:intergenic |
| TCAAATTTATCCACTGCCGC+AGG | 0.573609 | 2_4:+6633720 | Msa0311750:CDS |
| TTGTTTATCTGCTGACAGGA+AGG | 0.573644 | 2_4:-6633847 | None:intergenic |
| CTGAAAATATGTCCCAGTGA+AGG | 0.605019 | 2_4:-6634076 | None:intergenic |
| AAGAGCAAGCCTCACTTGTG+TGG | 0.630967 | 2_4:+6633776 | Msa0311750:CDS |
| AATGGAAACAGCACTGCAAG+AGG | 0.632913 | 2_4:+6633905 | Msa0311750:CDS |
| GCAACAAACAAACCTTCACT+GGG | 0.643067 | 2_4:+6634064 | Msa0311750:CDS |
| TATGAATTGCCACACAAGTG+AGG | 0.645881 | 2_4:-6633785 | None:intergenic |
| TGTTCCTATAATGTTGCTGG+AGG | 0.651755 | 2_4:+6633813 | Msa0311750:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAGATTTGAATTATTAAAA+AGG | - | chr2_4:6634014-6634033 | None:intergenic | 10.0% |
| !! | ATTTGAATTATTAAAAAGGT+GGG | - | chr2_4:6634010-6634029 | None:intergenic | 15.0% |
| !! | GATTTGAATTATTAAAAAGG+TGG | - | chr2_4:6634011-6634030 | None:intergenic | 20.0% |
| !! | TTGCATATAGAAAAATCATT+TGG | + | chr2_4:6634129-6634148 | Msa0311750:exon | 20.0% |
| !! | TTTTAATGAAATTGCACCAA+TGG | + | chr2_4:6633887-6633906 | Msa0311750:CDS | 25.0% |
| !! | AAATCTAATTGCGAAGATTT+TGG | + | chr2_4:6634026-6634045 | Msa0311750:intron | 25.0% |
| ! | GCTCTTTAGTAGACAATTTT+TGG | - | chr2_4:6633762-6633781 | None:intergenic | 30.0% |
| TTTCTATATGCAAGAATCCA+TGG | - | chr2_4:6634122-6634141 | None:intergenic | 30.0% | |
| !! | GACAATTTTTGGATCAGAAG+AGG | - | chr2_4:6633751-6633770 | None:intergenic | 35.0% |
| TAGTGTTCCTATAATGTTGC+TGG | + | chr2_4:6633810-6633829 | Msa0311750:CDS | 35.0% | |
| !!! | TTGCGAAGATTTTGGTTTCT+TGG | + | chr2_4:6634034-6634053 | Msa0311750:intron | 35.0% |
| !!! | AAGATTTTGGTTTCTTGGCT+TGG | + | chr2_4:6634039-6634058 | Msa0311750:intron | 35.0% |
| TTTCAGAGTGAGGAAATTCA+AGG | + | chr2_4:6634093-6634112 | Msa0311750:CDS | 35.0% | |
| ATCAGAAGAGGAAAAACCTG+CGG | - | chr2_4:6633739-6633758 | None:intergenic | 40.0% | |
| TATGAATTGCCACACAAGTG+AGG | - | chr2_4:6633788-6633807 | None:intergenic | 40.0% | |
| TGTTCCTATAATGTTGCTGG+AGG | + | chr2_4:6633813-6633832 | Msa0311750:CDS | 40.0% | |
| ACTTCCTCCAGCAACATTAT+AGG | - | chr2_4:6633820-6633839 | None:intergenic | 40.0% | |
| TTGTTTATCTGCTGACAGGA+AGG | - | chr2_4:6633850-6633869 | None:intergenic | 40.0% | |
| AGCAACAAACAAACCTTCAC+TGG | + | chr2_4:6634063-6634082 | Msa0311750:CDS | 40.0% | |
| GCAACAAACAAACCTTCACT+GGG | + | chr2_4:6634064-6634083 | Msa0311750:CDS | 40.0% | |
| CTGAAAATATGTCCCAGTGA+AGG | - | chr2_4:6634079-6634098 | None:intergenic | 40.0% | |
| ! | TGGGACATATTTTCAGAGTG+AGG | + | chr2_4:6634083-6634102 | Msa0311750:CDS | 40.0% |
| TCAAATTTATCCACTGCCGC+AGG | + | chr2_4:6633720-6633739 | Msa0311750:CDS | 45.0% | |
| CTGCTTGTTTATCTGCTGAC+AGG | - | chr2_4:6633854-6633873 | None:intergenic | 45.0% | |
| !! | TCTTGCAGTGCTGTTTCCAT+TGG | - | chr2_4:6633906-6633925 | None:intergenic | 45.0% |
| AATGGAAACAGCACTGCAAG+AGG | + | chr2_4:6633905-6633924 | Msa0311750:CDS | 45.0% | |
| GAGGAAATTCAAGGTTGCCA+TGG | + | chr2_4:6634102-6634121 | Msa0311750:CDS | 45.0% | |
| AAGAGCAAGCCTCACTTGTG+TGG | + | chr2_4:6633776-6633795 | Msa0311750:CDS | 50.0% | |
| TGTGTGTGTGTGTGTGTGTG+GGG | - | chr2_4:6633985-6634004 | None:intergenic | 50.0% | |
| GTGTGTGTGTGTGTGTGTGT+GGG | - | chr2_4:6633986-6634005 | None:intergenic | 50.0% | |
| TGTGTGTGTGTGTGTGTGTG+TGG | - | chr2_4:6633985-6634004 | None:intergenic | 50.0% | |
| AGAGGAAAAACCTGCGGCAG+TGG | - | chr2_4:6633733-6633752 | None:intergenic | 55.0% | |
| TGTGTGTGTGTGTGTGTGGG+GGG | - | chr2_4:6633983-6634002 | None:intergenic | 55.0% | |
| GTGTGTGTGTGTGTGTGTGG+GGG | - | chr2_4:6633984-6634003 | None:intergenic | 55.0% | |
| TGTGTGTGTGTGTGTGGGGG+GGG | - | chr2_4:6633981-6634000 | None:intergenic | 60.0% | |
| GTGTGTGTGTGTGTGTGGGG+GGG | - | chr2_4:6633982-6634001 | None:intergenic | 60.0% | |
| TGTGTGTGTGTGTGGGGGGG+GGG | - | chr2_4:6633979-6633998 | None:intergenic | 65.0% | |
| GTGTGTGTGTGTGTGGGGGG+GGG | - | chr2_4:6633980-6633999 | None:intergenic | 65.0% | |
| TGTGTGTGTGTGGGGGGGGG+GGG | - | chr2_4:6633977-6633996 | None:intergenic | 70.0% | |
| GTGTGTGTGTGTGGGGGGGG+GGG | - | chr2_4:6633978-6633997 | None:intergenic | 70.0% | |
| TGTGTGTGTGGGGGGGGGGG+GGG | - | chr2_4:6633975-6633994 | None:intergenic | 75.0% | |
| GTGTGTGTGTGGGGGGGGGG+GGG | - | chr2_4:6633976-6633995 | None:intergenic | 75.0% | |
| ! | TGTGTGTGGGGGGGGGGGGG+GGG | - | chr2_4:6633973-6633992 | None:intergenic | 80.0% |
| ! | GTGTGTGTGGGGGGGGGGGG+GGG | - | chr2_4:6633974-6633993 | None:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2_4 | gene | 6633691 | 6634167 | 6633691 | ID=Msa0311750;Name=Msa0311750 |
| chr2_4 | mRNA | 6633691 | 6634167 | 6633691 | ID=Msa0311750-mRNA-1;Parent=Msa0311750;Name=Msa0311750-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|1|0.5|1|0|0.5|2|29|110 |
| chr2_4 | exon | 6633691 | 6633926 | 6633691 | ID=Msa0311750-mRNA-1:exon:2547;Parent=Msa0311750-mRNA-1 |
| chr2_4 | exon | 6634042 | 6634167 | 6634042 | ID=Msa0311750-mRNA-1:exon:2548;Parent=Msa0311750-mRNA-1 |
| chr2_4 | CDS | 6633691 | 6633926 | 6633691 | ID=Msa0311750-mRNA-1:cds;Parent=Msa0311750-mRNA-1 |
| chr2_4 | CDS | 6634042 | 6634138 | 6634042 | ID=Msa0311750-mRNA-1:cds;Parent=Msa0311750-mRNA-1 |
| chr2_4 | three_prime_UTR | 6634139 | 6634167 | 6634139 | ID=Msa0311750-mRNA-1:three_prime_utr;Parent=Msa0311750-mRNA-1 |
| Gene Sequence |
| Protein sequence |
