AlfalfaGEDB | Msa0311750

M. sativa cultivar zhongmu-4 / Msa0311750

KEGG: -
GO: -
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0311750	XP_013457864.1	100.000	35	0	0	17	51	173	207	1.41e-11	71.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0142220	Msa0311750	0.833329	5.676214e-56	-8.615850e-47
Msa0232850	Msa0311750	0.800658	1.299580e-48	-8.615850e-47
Msa0251320	Msa0311750	0.810527	1.102495e-50	-8.615850e-47
Msa0311750	Msa0349360	0.800218	1.597716e-48	-8.615850e-47
Msa0311750	Msa0360510	0.809016	2.330261e-50	-8.615850e-47
Msa0311750	Msa0384800	0.835892	1.287609e-56	-8.615850e-47
Msa0311750	Msa0390070	0.802313	5.953327e-49	-8.615850e-47
Msa0311750	Msa0428000	0.811446	6.972711e-51	-8.615850e-47
Msa0311750	Msa0435230	0.816566	5.177575e-52	-8.615850e-47
Msa0311750	Msa0436780	0.819521	1.111986e-52	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0311750	MtrunA17_Chr4g0059881	100.000	35	0	0	17	51	173	207	1.29e-15	71.2

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Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa0311750	AT1G74770.1	77.143	35	8	17	51	165	199	1.71e-11	59.7
Msa0311750	AT1G74770.2	77.143	35	8	17	51	165	199	1.86e-11	59.7
Msa0311750	AT1G18910.1	71.429	35	10	17	51	173	207	7.19e-11	58.2

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Find 18 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGAGCAAGCCTCACTTGTG+TGG	0.630967	2_4:+6633776	Msa0311750:CDS
AATGGAAACAGCACTGCAAG+AGG	0.632913	2_4:+6633905	Msa0311750:CDS
ACTTCCTCCAGCAACATTAT+AGG	0.279336	2_4:-6633817	None:intergenic
AGCAACAAACAAACCTTCAC+TGG	0.431741	2_4:+6634063	Msa0311750:CDS
CTGAAAATATGTCCCAGTGA+AGG	0.605019	2_4:-6634076	None:intergenic
CTGCTTGTTTATCTGCTGAC+AGG	0.474479	2_4:-6633851	None:intergenic
GAGGAAATTCAAGGTTGCCA+TGG	0.554452	2_4:+6634102	Msa0311750:CDS
GAGTTCCTATTGAATGAACT+CGG	0.571210	2_4:-6634153	None:intergenic
GCAACAAACAAACCTTCACT+GGG	0.643067	2_4:+6634064	Msa0311750:CDS
TAGTGTTCCTATAATGTTGC+TGG	0.432236	2_4:+6633810	Msa0311750:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTCTATATGCAAGAATCCA+TGG	-	chr2_4:6634122-6634141	None:intergenic	30.0%
TAGTGTTCCTATAATGTTGC+TGG	+	chr2_4:6633810-6633829	Msa0311750:CDS	35.0%
TTTCAGAGTGAGGAAATTCA+AGG	+	chr2_4:6634093-6634112	Msa0311750:CDS	35.0%
ATCAGAAGAGGAAAAACCTG+CGG	-	chr2_4:6633739-6633758	None:intergenic	40.0%
TATGAATTGCCACACAAGTG+AGG	-	chr2_4:6633788-6633807	None:intergenic	40.0%
TGTTCCTATAATGTTGCTGG+AGG	+	chr2_4:6633813-6633832	Msa0311750:CDS	40.0%
ACTTCCTCCAGCAACATTAT+AGG	-	chr2_4:6633820-6633839	None:intergenic	40.0%
TTGTTTATCTGCTGACAGGA+AGG	-	chr2_4:6633850-6633869	None:intergenic	40.0%
AGCAACAAACAAACCTTCAC+TGG	+	chr2_4:6634063-6634082	Msa0311750:CDS	40.0%
GCAACAAACAAACCTTCACT+GGG	+	chr2_4:6634064-6634083	Msa0311750:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2_4	gene	6633691	6634167	6633691	ID=Msa0311750;Name=Msa0311750
chr2_4	mRNA	6633691	6634167	6633691	ID=Msa0311750-mRNA-1;Parent=Msa0311750;Name=Msa0311750-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0\|1\|0.5\|1\|0\|0.5\|2\|29\|110
chr2_4	exon	6633691	6633926	6633691	ID=Msa0311750-mRNA-1:exon:2547;Parent=Msa0311750-mRNA-1
chr2_4	exon	6634042	6634167	6634042	ID=Msa0311750-mRNA-1:exon:2548;Parent=Msa0311750-mRNA-1
chr2_4	CDS	6633691	6633926	6633691	ID=Msa0311750-mRNA-1:cds;Parent=Msa0311750-mRNA-1
chr2_4	CDS	6634042	6634138	6634042	ID=Msa0311750-mRNA-1:cds;Parent=Msa0311750-mRNA-1
chr2_4	three_prime_UTR	6634139	6634167	6634139	ID=Msa0311750-mRNA-1:three_prime_utr;Parent=Msa0311750-mRNA-1

Gene Sequence	>Msa0311750 TACTTGAGTTTTGATTATCATACTTACTATCAAATTTATCCACTGCCGCAGGTTTTTCCTCTTCTGATCCAAAAATTGTCTACTAAAGAGCAAGCCTCACTTGTGTGGCAATTCATATGTAGTGTTCCTATAATGTTGCTGGAGGAAGTTTTGTGTCCTTCCTGTCAGCAGATAAACAAGCAGAAGCTACTTAGTGTTTTAATGAAATTGCACCAATGGAAACAGCACTGCAAGAGATTTTGGTTTCTTGGCTTGGAAGCAACAAACAAACCTTCACTGGGACATATTTTCAGAGTGAGGAAATTCAAGGTTGCCATGGATTCTTGCATATAG
Protein sequence	>Msa0311750 YLSFDYHTYYQIYPLPQVFPLLIQKLSTKEQASLVWQFICSVPIMLLEEVLCPSCQQINKQKLLSVLMKLHQWKQHCKRFWFLGLEATNKPSLGHIFRVRKFKVAMDSCI*