AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1336960

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1336960 MtrunA17_Chr6g0451841 98.155 542 9 1 1 542 1 541 0.0 1090
Msa1336960 MtrunA17_Chr4g0014871 38.745 542 292 10 12 537 37 554 7.26e-130 390
Msa1336960 MtrunA17_Chr4g0014841 37.803 537 301 7 12 537 36 550 7.80e-127 382
Msa1336960 MtrunA17_Chr4g0014781 38.333 540 302 7 12 540 36 555 3.40e-126 380
Msa1336960 MtrunA17_Chr4g0014791 37.616 537 302 9 12 537 36 550 2.77e-125 378
Msa1336960 MtrunA17_Chr3g0077151 38.078 541 294 9 12 540 35 546 5.06e-125 377
Msa1336960 MtrunA17_Chr4g0014801 37.523 549 308 8 4 537 24 552 2.32e-122 370
Msa1336960 MtrunA17_Chr8g0353031 35.599 559 305 14 12 542 25 556 3.25e-114 350
Msa1336960 MtrunA17_Chr4g0008651 34.901 553 311 10 16 542 45 574 1.20e-113 349
Msa1336960 MtrunA17_Chr4g0005901 36.347 553 303 9 13 537 40 571 1.18e-111 343
Msa1336960 MtrunA17_Chr7g0240381 34.358 553 308 10 12 538 36 559 4.20e-110 340
Msa1336960 MtrunA17_Chr3g0112671 35.556 540 293 10 24 538 52 561 3.18e-109 337
Msa1336960 MtrunA17_Chr4g0014861 39.294 425 238 7 119 537 19 429 1.28e-108 331
Msa1336960 MtrunA17_Chr7g0241741 34.720 553 312 11 13 537 30 561 2.10e-108 335
Msa1336960 MtrunA17_Chr8g0375311 33.986 562 315 11 12 540 47 585 2.78e-106 330
Msa1336960 MtrunA17_Chr3g0112611 34.120 551 312 12 16 538 43 570 3.76e-106 330
Msa1336960 MtrunA17_Chr3g0114501 35.145 552 319 9 12 540 29 564 9.24e-106 329
Msa1336960 MtrunA17_Chr8g0375291 34.483 551 301 12 12 527 47 572 9.17e-102 318
Msa1336960 MtrunA17_Chr3g0104011 32.374 556 326 10 13 538 30 565 1.25e-98 310
Msa1336960 MtrunA17_Chr8g0375341 33.746 566 311 11 12 540 47 585 2.72e-98 309
Msa1336960 MtrunA17_Chr8g0375351 33.514 555 315 11 12 534 47 579 3.09e-98 309
Msa1336960 MtrunA17_Chr7g0261881 32.188 553 328 11 12 536 34 567 1.12e-95 303
Msa1336960 MtrunA17_Chr7g0261771 33.095 559 326 13 6 536 33 571 4.64e-95 301
Msa1336960 MtrunA17_Chr7g0261811 32.007 553 329 10 12 536 34 567 9.53e-95 300
Msa1336960 MtrunA17_Chr1g0187241 32.028 562 329 14 12 542 33 572 1.10e-93 297
Msa1336960 MtrunA17_Chr3g0142101 32.537 544 313 13 23 540 53 568 2.08e-91 291
Msa1336960 MtrunA17_Chr7g0261791 31.560 564 328 11 12 541 36 575 3.87e-91 291
Msa1336960 MtrunA17_Chr1g0166711 33.452 562 320 15 12 542 32 570 4.41e-91 291
Msa1336960 MtrunA17_Chr8g0375321 32.086 561 327 10 12 540 44 582 8.04e-91 290
Msa1336960 MtrunA17_Chr8g0375331 32.270 533 307 10 12 512 31 541 7.00e-88 281
Msa1336960 MtrunA17_Chr1g0166741 31.485 559 322 14 12 539 34 562 9.55e-88 281
Msa1336960 MtrunA17_Chr3g0082631 36.142 451 240 11 119 541 33 463 7.69e-87 276
Msa1336960 MtrunA17_Chr7g0261851 31.272 566 339 12 6 542 30 574 6.24e-86 277
Msa1336960 MtrunA17_Chr8g0375361 32.079 558 325 11 12 537 46 581 1.14e-84 274
Msa1336960 MtrunA17_Chr7g0217461 31.720 558 320 9 15 534 34 568 2.16e-83 270
Msa1336960 MtrunA17_Chr1g0166791 30.088 565 336 14 12 542 31 570 8.80e-83 269
Msa1336960 MtrunA17_Chr5g0399061 33.088 544 323 11 23 539 45 574 1.91e-82 268
Msa1336960 MtrunA17_Chr1g0166771 31.629 528 300 14 24 520 10 507 2.75e-82 266
Msa1336960 MtrunA17_Chr5g0399071 32.554 556 310 14 23 539 43 572 1.54e-81 266
Msa1336960 MtrunA17_Chr7g0255621 30.220 546 327 10 24 536 39 563 1.59e-81 266
Msa1336960 MtrunA17_Chr7g0261661 32.373 451 257 9 108 536 29 453 2.02e-81 263
Msa1336960 MtrunA17_Chr5g0415791 30.319 564 317 10 24 541 48 581 2.58e-81 265
Msa1336960 MtrunA17_Chr4g0056431 30.796 578 316 8 12 539 32 575 3.21e-81 265
Msa1336960 MtrunA17_Chr4g0054891 31.418 557 319 11 16 536 121 650 3.65e-81 266
Msa1336960 MtrunA17_Chr8g0362711 31.720 558 326 11 4 527 25 561 1.21e-80 263
Msa1336960 MtrunA17_Chr5g0422881 29.872 549 344 8 24 542 39 576 6.78e-80 261
Msa1336960 MtrunA17_Chr7g0214461 28.391 553 352 11 6 534 79 611 3.74e-77 255
Msa1336960 MtrunA17_Chr3g0142091 31.181 542 320 10 23 537 44 559 2.76e-75 249
Msa1336960 MtrunA17_Chr5g0400371 29.735 565 338 12 6 534 114 655 4.80e-75 251
Msa1336960 MtrunA17_Chr1g0213711 29.250 547 335 12 16 534 32 554 7.23e-75 248
Msa1336960 MtrunA17_Chr7g0255601 29.358 545 331 10 24 535 46 569 8.32e-75 248
Msa1336960 MtrunA17_Chr5g0415961 30.530 547 326 12 16 527 35 562 1.12e-74 247
Msa1336960 MtrunA17_Chr1g0166731 34.163 442 249 12 120 542 49 467 1.51e-73 241
Msa1336960 MtrunA17_Chr4g0059991 29.928 558 330 11 24 541 41 577 7.61e-73 243
Msa1336960 MtrunA17_Chr4g0059971 28.388 546 339 11 24 534 53 581 4.34e-72 241
Msa1336960 MtrunA17_Chr4g0059981 30.521 557 330 15 24 542 50 587 9.43e-72 240
Msa1336960 MtrunA17_Chr7g0255611 29.725 545 329 11 24 535 48 571 3.36e-70 236
Msa1336960 MtrunA17_Chr4g0063591 30.601 549 335 10 16 539 33 560 1.02e-69 234
Msa1336960 MtrunA17_Chr2g0276751 28.857 551 344 11 11 540 44 567 1.52e-69 234
Msa1336960 MtrunA17_Chr8g0362781 28.098 573 321 11 23 540 48 584 1.64e-69 234
Msa1336960 MtrunA17_Chr8g0390331 30.182 550 324 15 24 539 34 557 1.94e-67 228
Msa1336960 MtrunA17_Chr2g0284701 26.620 571 359 10 12 540 39 591 2.51e-67 228
Msa1336960 MtrunA17_Chr7g0218851 27.128 564 356 14 1 534 43 581 3.32e-67 228
Msa1336960 MtrunA17_Chr4g0011471 29.358 545 322 9 27 534 38 556 4.90e-67 227
Msa1336960 MtrunA17_Chr7g0261751 30.040 496 270 10 6 441 33 511 2.11e-64 219
Msa1336960 MtrunA17_Chr5g0439381 28.269 566 311 13 24 541 47 565 2.84e-64 220
Msa1336960 MtrunA17_Chr1g0157601 28.029 553 339 13 16 534 26 553 3.10e-63 216
Msa1336960 MtrunA17_Chr1g0193391 29.032 558 353 12 5 542 17 551 3.20e-63 217
Msa1336960 MtrunA17_Chr7g0271221 29.295 553 358 10 5 542 17 551 3.84e-63 217
Msa1336960 MtrunA17_Chr3g0112631 40.417 240 142 1 299 538 35 273 6.57e-63 208
Msa1336960 MtrunA17_Chr2g0331521 28.125 576 351 16 5 542 20 570 9.26e-63 216
Msa1336960 MtrunA17_Chr4g0011041 28.700 554 346 13 12 534 43 578 1.26e-61 213
Msa1336960 MtrunA17_Chr4g0003761 28.470 562 333 16 16 538 41 572 1.46e-61 213
Msa1336960 MtrunA17_Chr3g0133341 28.042 567 348 12 8 541 15 554 2.50e-61 211
Msa1336960 MtrunA17_Chr1g0157611 29.068 547 341 11 16 534 27 554 4.22e-61 211
Msa1336960 MtrunA17_Chr1g0147631 27.455 550 347 11 16 534 36 564 1.03e-60 210
Msa1336960 MtrunA17_Chr8g0379971 27.222 540 321 12 22 506 37 559 1.07e-60 211
Msa1336960 MtrunA17_Chr1g0177591 28.839 534 322 15 30 538 67 567 6.90e-60 207
Msa1336960 MtrunA17_Chr2g0331471 27.641 568 357 16 5 538 85 632 1.54e-59 209
Msa1336960 MtrunA17_Chr4g0060001 28.080 552 326 14 24 534 48 569 2.01e-59 207
Msa1336960 MtrunA17_Chr2g0331481 27.448 572 361 16 1 538 18 569 1.12e-58 206
Msa1336960 MtrunA17_Chr1g0177631 31.891 439 243 14 123 538 33 438 6.07e-58 199
Msa1336960 MtrunA17_Chr8g0362801 27.533 454 281 8 108 537 32 461 7.51e-58 200
Msa1336960 MtrunA17_Chr8g0379991 27.072 543 315 11 30 506 5 532 1.46e-57 201
Msa1336960 MtrunA17_Chr1g0157621 29.220 551 306 14 30 534 5 517 1.62e-57 201
Msa1336960 MtrunA17_Chr1g0177611 30.524 439 249 14 123 538 33 438 1.27e-54 190
Msa1336960 MtrunA17_Chr5g0415841 28.636 440 267 10 120 535 42 458 2.62e-54 190
Msa1336960 MtrunA17_Chr7g0214731 26.277 548 359 11 24 539 80 614 4.78e-54 193
Msa1336960 MtrunA17_Chr2g0331511 26.425 579 357 16 1 538 15 565 5.98e-54 192
Msa1336960 MtrunA17_Chr7g0240271 26.557 546 349 11 24 536 43 569 7.14e-54 192
Msa1336960 MtrunA17_Chr4g0069581 26.047 549 341 12 23 539 4 519 3.31e-53 189
Msa1336960 MtrunA17_Chr2g0331461 26.455 567 367 14 1 535 18 566 3.15e-51 185
Msa1336960 MtrunA17_Chr3g0142121 34.601 263 161 3 278 540 19 270 1.94e-50 174
Msa1336960 MtrunA17_Chr2g0326211 25.400 563 360 10 1 536 16 545 1.90e-48 176
Msa1336960 MtrunA17_Chr2g0325961 25.872 545 343 12 20 536 35 546 4.40e-48 175
Msa1336960 MtrunA17_Chr2g0325951 25.187 536 344 11 27 536 41 545 4.46e-48 175
Msa1336960 MtrunA17_Chr7g0234181 30.913 427 248 11 116 534 26 413 2.35e-47 170
Msa1336960 MtrunA17_Chr4g0060071 34.328 268 169 4 277 541 39 302 3.66e-47 166
Msa1336960 MtrunA17_Chr7g0240281 25.180 556 342 10 24 534 43 569 8.68e-47 172
Msa1336960 MtrunA17_Chr7g0240131 27.578 446 275 12 115 534 33 456 4.59e-44 162
Msa1336960 MtrunA17_Chr7g0235521 27.578 446 275 12 115 534 33 456 1.51e-43 161
Msa1336960 MtrunA17_Chr7g0240181 27.314 443 280 11 115 534 33 456 4.04e-43 160
Msa1336960 MtrunA17_Chr7g0240211 27.314 443 280 11 115 534 33 456 4.16e-43 160
Msa1336960 MtrunA17_Chr7g0261721 36.634 202 126 2 336 536 1 201 1.23e-42 152
Msa1336960 MtrunA17_Chr7g0234151 29.358 436 251 9 112 534 27 418 1.76e-40 151
Msa1336960 MtrunA17_Chr7g0234381 28.842 423 262 8 116 534 31 418 3.28e-40 150
Msa1336960 MtrunA17_Chr7g0240151 26.269 453 254 13 115 534 33 438 4.84e-38 145
Msa1336960 MtrunA17_Chr7g0261731 29.641 334 179 9 6 307 33 342 1.03e-36 139
Msa1336960 MtrunA17_Chr3g0142111 34.322 236 129 4 23 248 64 283 2.45e-33 129
Msa1336960 MtrunA17_Chr2g0326221 25.943 212 126 5 328 539 44 224 3.11e-20 89.7
Msa1336960 MtrunA17_Chr3g0112651 36.752 117 66 1 110 226 46 154 1.07e-19 87.8
Msa1336960 MtrunA17_Chr4g0060061 33.858 127 69 2 114 240 16 127 1.36e-13 68.2
Msa1336960 MtrunA17_Chr1g0147591 32.292 96 60 1 123 218 9 99 2.83e-12 65.9
Msa1336960 MtrunA17_Chr3g0082621 44.118 68 38 0 24 91 5 72 6.96e-12 61.6
Msa1336960 MtrunA17_Chr7g0261831 26.712 146 92 3 254 384 14 159 8.14e-12 65.1
Msa1336960 MtrunA17_Chr4g0014851 40.506 79 47 0 13 91 38 116 1.77e-11 62.8
Msa1336960 MtrunA17_Chr3g0112661 44.118 68 38 0 24 91 50 117 2.90e-11 61.6
Msa1336960 MtrunA17_Chr1g0147581 30.579 121 78 2 390 510 1 115 9.20e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1336960 AT5G14940.1 61.290 558 186 11 1 541 1 545 0.0 650
Msa1336960 AT3G01350.1 60.036 553 201 8 5 541 5 553 0.0 612
Msa1336960 AT1G22540.1 41.031 524 281 6 24 537 47 552 1.59e-130 392
Msa1336960 AT1G72140.1 38.910 532 276 11 24 536 51 552 1.63e-124 376
Msa1336960 AT1G72120.1 39.252 535 294 8 12 534 34 549 2.85e-123 373
Msa1336960 AT1G72125.1 39.400 533 276 10 24 534 46 553 2.14e-122 371
Msa1336960 AT1G72130.1 36.531 542 311 8 2 540 26 537 1.29e-120 366
Msa1336960 AT3G54450.1 36.106 565 305 14 5 541 10 546 3.80e-118 360
Msa1336960 AT1G22550.1 37.476 531 291 9 24 534 47 556 2.20e-114 350
Msa1336960 AT3G53960.1 34.403 561 306 11 12 538 42 574 5.73e-106 330
Msa1336960 AT2G37900.1 33.810 559 312 10 12 542 43 571 1.51e-105 328
Msa1336960 AT1G22570.2 36.240 516 281 12 24 515 10 501 4.93e-104 323
Msa1336960 AT1G22570.1 35.795 528 291 12 12 515 35 538 1.04e-103 323
Msa1336960 AT5G01180.2 32.740 562 323 12 13 542 31 569 1.90e-102 320
Msa1336960 AT5G01180.3 32.740 562 323 12 13 542 31 569 1.90e-102 320
Msa1336960 AT5G01180.1 32.740 562 323 12 13 542 31 569 1.90e-102 320
Msa1336960 AT3G54140.1 33.393 557 319 10 13 538 30 565 1.38e-101 318
Msa1336960 AT3G54140.2 33.393 557 319 10 13 538 30 565 1.38e-101 318
Msa1336960 AT2G40460.1 33.690 561 324 11 6 542 24 560 3.17e-101 317
Msa1336960 AT1G72130.2 36.557 424 243 6 119 540 20 419 4.89e-99 306
Msa1336960 AT1G72130.3 36.557 424 243 6 119 540 20 419 4.89e-99 306
Msa1336960 AT2G37900.2 33.973 521 286 10 50 542 2 492 3.58e-98 306
Msa1336960 AT2G02040.1 32.793 555 331 10 12 540 46 584 3.71e-97 306
Msa1336960 AT1G62200.1 32.916 559 314 8 12 537 57 587 2.92e-94 299
Msa1336960 AT2G02020.1 33.766 539 303 12 12 537 47 544 8.31e-93 294
Msa1336960 AT1G62200.2 32.007 578 323 9 12 537 57 616 1.02e-90 291
Msa1336960 AT1G62200.3 32.007 578 323 9 12 537 71 630 1.80e-90 291
Msa1336960 AT5G46040.1 30.864 567 341 10 4 539 20 566 2.39e-84 273
Msa1336960 AT1G27040.3 31.801 544 329 9 14 534 2 526 7.54e-82 265
Msa1336960 AT2G02020.2 32.653 539 288 14 12 537 47 523 1.26e-81 264
Msa1336960 AT1G27040.2 31.949 554 333 10 4 534 22 554 1.31e-81 265
Msa1336960 AT1G27040.1 31.949 554 333 10 4 534 26 558 1.64e-81 265
Msa1336960 AT5G46050.1 30.125 561 340 12 12 542 32 570 1.21e-80 263
Msa1336960 AT1G69850.1 31.218 583 336 15 4 542 24 585 7.88e-79 259
Msa1336960 AT1G69870.1 29.529 552 328 9 23 537 67 594 4.85e-77 255
Msa1336960 AT4G21680.1 31.228 570 324 14 13 540 34 577 8.44e-77 253
Msa1336960 AT1G32450.1 31.103 553 332 11 15 534 45 581 1.66e-76 253
Msa1336960 AT2G26690.1 30.199 553 337 11 13 539 30 559 3.78e-76 251
Msa1336960 AT2G26690.2 30.387 543 329 11 23 539 7 526 4.38e-76 250
Msa1336960 AT1G68570.2 30.165 547 331 10 24 537 10 538 2.86e-75 249
Msa1336960 AT1G68570.1 30.165 547 331 10 24 537 40 568 6.09e-75 249
Msa1336960 AT2G26690.3 30.410 536 324 11 30 539 2 514 1.56e-74 246
Msa1336960 AT5G62730.2 29.144 549 338 11 16 539 6 528 1.72e-73 243
Msa1336960 AT5G62730.1 29.055 561 345 12 4 539 55 587 4.10e-73 244
Msa1336960 AT5G19640.1 28.571 567 324 13 13 541 65 588 1.72e-72 243
Msa1336960 AT1G12110.1 29.239 578 319 12 13 539 33 571 2.68e-71 239
Msa1336960 AT5G46050.2 31.293 441 263 10 120 542 40 458 7.53e-69 229
Msa1336960 AT1G18880.1 27.768 551 341 11 24 537 36 566 1.81e-67 229
Msa1336960 AT1G59740.1 28.877 561 336 12 18 542 49 582 5.00e-67 228
Msa1336960 AT3G21670.1 29.380 548 322 12 24 539 46 560 7.12e-66 224
Msa1336960 AT3G16180.1 29.340 576 337 18 7 538 19 568 1.31e-65 224
Msa1336960 AT1G52190.1 28.299 576 342 16 4 535 18 566 5.01e-62 214
Msa1336960 AT1G33440.1 27.143 560 338 12 11 534 42 567 5.01e-62 214
Msa1336960 AT3G25260.1 27.810 525 328 11 19 534 33 515 9.92e-61 209
Msa1336960 AT1G27080.1 27.387 555 335 11 23 541 30 552 8.06e-60 208
Msa1336960 AT5G62680.1 25.355 564 348 10 24 541 60 596 3.55e-58 204
Msa1336960 AT1G27080.2 27.407 540 324 11 38 541 3 510 4.71e-57 199
Msa1336960 AT5G13400.1 26.703 558 347 10 24 541 81 616 6.34e-57 201
Msa1336960 AT1G69860.1 27.289 546 344 14 23 541 34 553 4.47e-56 197
Msa1336960 AT3G47960.1 25.405 555 357 11 24 541 78 612 1.41e-55 197
Msa1336960 AT3G45720.1 25.603 539 333 12 34 542 46 546 1.98e-54 193
Msa1336960 AT5G28470.2 28.073 545 343 14 24 539 34 558 1.59e-53 191
Msa1336960 AT5G11570.1 27.106 546 315 12 1 537 7 478 1.53e-52 186
Msa1336960 AT3G45660.1 26.916 535 325 14 30 536 44 540 2.17e-52 187
Msa1336960 AT3G45660.3 26.916 535 325 14 30 536 44 540 2.17e-52 187
Msa1336960 AT3G45660.2 26.916 535 325 14 30 536 65 561 3.75e-52 187
Msa1336960 AT3G45650.1 26.692 532 322 14 34 536 49 541 8.86e-52 186
Msa1336960 AT3G45680.1 24.156 563 350 12 16 541 30 552 1.07e-50 183
Msa1336960 AT5G28470.1 27.890 545 342 15 24 539 34 556 1.12e-50 182
Msa1336960 AT3G25280.1 25.898 529 345 9 16 534 30 521 6.66e-50 180
Msa1336960 AT3G45700.1 24.632 544 346 14 16 535 26 529 3.58e-47 173
Msa1336960 AT3G45650.2 25.806 558 320 16 34 536 49 567 1.19e-46 172
Msa1336960 AT3G45710.1 22.727 550 356 13 16 535 31 541 1.00e-44 166
Msa1336960 AT3G45690.1 21.550 529 311 13 34 535 45 496 3.10e-31 127
Msa1336960 AT2G38100.2 25.926 540 317 19 35 541 2 491 4.59e-31 127
Msa1336960 AT2G38100.1 25.926 540 317 19 35 541 18 507 5.89e-31 126
Msa1336960 AT2G38100.3 25.926 540 317 19 35 541 24 513 9.00e-31 126

Find 95 sgRNAs with CRISPR-Local

Find 257 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTTTCAATGGTGGTATTT+TGG 0.145365 tig0001334:-74432 Msa1336960:CDS
CACCTATTGCTGATGCTTAT+TGG 0.205664 tig0001334:-76534 Msa1336960:CDS
GACACATTTGGTTGGGTAAT+AGG 0.231752 tig0001334:-74360 Msa1336960:CDS
CAAAGGTGTAGCCTCAAATT+TGG 0.243808 tig0001334:-76656 Msa1336960:CDS
TGATTAAGCTATTGCCTATA+TGG 0.259511 tig0001334:-73345 Msa1336960:CDS
ACCTATTGCTGATGCTTATT+GGG 0.269588 tig0001334:-76533 Msa1336960:CDS
TTAAGGTACTATACAAATAT+TGG 0.271436 tig0001334:+77475 None:intergenic
CATTTGGTATCTTCTTATAA+TGG 0.282553 tig0001334:-77360 Msa1336960:exon
TTACCACAGTACATTCTTCT+TGG 0.292712 tig0001334:-72953 Msa1336960:CDS
ACACATTTGGTTGGGTAATA+GGG 0.306033 tig0001334:-74359 Msa1336960:CDS
TCTCTCTTAAGTTTGGATAT+TGG 0.309812 tig0001334:-77403 Msa1336960:five_prime_UTR
TAACGGCATTAACAGTATTA+TGG 0.311123 tig0001334:+73186 None:intergenic
AACAACCAGAAAATGCTTAT+TGG 0.321275 tig0001334:+72974 None:intergenic
CTGATAACACGACAGGAAAA+AGG 0.335435 tig0001334:-73133 Msa1336960:CDS
TCTCTCTATTTAATCTCATT+AGG 0.346870 tig0001334:-74567 Msa1336960:CDS
GTTTCCACATGATCATGTTT+TGG 0.359186 tig0001334:+77447 None:intergenic
CCCATTGTTCTCATATTCTT+CGG 0.363879 tig0001334:+72875 None:intergenic
GAGAAAAGATGAGGACATTA+TGG 0.364757 tig0001334:+76498 None:intergenic
AAGCATTAAGGTCATAAATA+AGG 0.365962 tig0001334:-73410 Msa1336960:CDS
TGCCACCAACAATGGCATTA+TGG 0.367205 tig0001334:+76555 None:intergenic
TGTTTGTGCTGGAAGCCTAA+TGG 0.372172 tig0001334:-74409 Msa1336960:CDS
GCTATATTAATATGATATAA+TGG 0.375823 tig0001334:+72547 None:intergenic
CACTTCCATAATGCCATTGT+TGG 0.382096 tig0001334:-76560 Msa1336960:CDS
CACGTTTGGATAATTACTAT+TGG 0.386424 tig0001334:-72727 Msa1336960:CDS
CGAAAACTGTGAAAGCATTA+AGG 0.391903 tig0001334:-73422 Msa1336960:CDS
CAGCAAAGATGGTTAATAGT+TGG 0.392002 tig0001334:-76591 Msa1336960:CDS
TGTAACAGAGTTAGGACTAT+TGG 0.407424 tig0001334:-77525 Msa1336960:five_prime_UTR
GTTTGTGCTGGAAGCCTAAT+GGG 0.410630 tig0001334:-74408 Msa1336960:CDS
TTAATCATAACCTTTACATT+TGG 0.412053 tig0001334:+73361 None:intergenic
TGGTATCTTCTTATAATGGC+TGG 0.414363 tig0001334:-77356 Msa1336960:exon
CTCAAAGTTGTAACAGAGTT+AGG 0.419150 tig0001334:-77533 Msa1336960:five_prime_UTR
AATCCATCTCTACAAGCCTT+TGG 0.428240 tig0001334:-74531 Msa1336960:CDS
TCTTACATCCAAGACACATT+TGG 0.430307 tig0001334:-74372 Msa1336960:CDS
TAGATAAGTTACCAAATTTG+AGG 0.430421 tig0001334:+76645 None:intergenic
AGCAAAGATGGTTAATAGTT+GGG 0.441043 tig0001334:-76590 Msa1336960:CDS
CATCCAAGACACATTTGGTT+GGG 0.442138 tig0001334:-74367 Msa1336960:CDS
CAACTCATCTGCAGCAAAGA+TGG 0.448211 tig0001334:-76602 Msa1336960:CDS
ATGAGAAGCATTGTCCATAT+AGG 0.448966 tig0001334:+73331 None:intergenic
TGATATGGTCGAGGCACGTT+TGG 0.450686 tig0001334:-72741 Msa1336960:CDS
AATTCATGCATTCTCCTCAT+AGG 0.453128 tig0001334:-77308 Msa1336960:intron
ATTACTATTGGCTTCTAGCT+TGG 0.455302 tig0001334:-72715 Msa1336960:CDS
GAAGTATACACAAGTTCAAA+AGG 0.460982 tig0001334:-72785 Msa1336960:CDS
GCCCAATAAGCATCAGCAAT+AGG 0.462037 tig0001334:+76532 None:intergenic
ACATGAATGTTACACCCATT+AGG 0.462461 tig0001334:+74394 None:intergenic
CTATTTGAAACAGTCATTGC+AGG 0.464469 tig0001334:-76697 Msa1336960:intron
CCGAAGAATATGAGAACAAT+GGG 0.466258 tig0001334:-72875 Msa1336960:CDS
GCCTTTGGAGCTGATCAACT+TGG 0.472222 tig0001334:-74516 Msa1336960:CDS
GCATGAATTGCTGAGCCTCT+TGG 0.476082 tig0001334:+77322 None:intergenic
AAGATGGTTAATAGTTGGGT+TGG 0.477748 tig0001334:-76586 Msa1336960:CDS
TGGACAATGTTATAGCACTT+TGG 0.479628 tig0001334:+73206 None:intergenic
GCTCCAAAGGCTTGTAGAGA+TGG 0.488186 tig0001334:+74528 None:intergenic
TTCCATAATGCCATTGTTGG+TGG 0.499004 tig0001334:-76557 Msa1336960:CDS
CACTTTGGAGTGTGGCCGGT+GGG 0.502069 tig0001334:+73221 None:intergenic
TTACCCAACCAAATGTGTCT+TGG 0.508468 tig0001334:+74364 None:intergenic
CTCTTAAACATGTTCGTGAA+AGG 0.511777 tig0001334:+74225 None:intergenic
TGCTATACCTGCGATTTCGA+TGG 0.512758 tig0001334:-74334 Msa1336960:CDS
ACATCCAAGACACATTTGGT+TGG 0.514558 tig0001334:-74368 Msa1336960:CDS
GAAATGAAACGCCTTGCTAT+CGG 0.516183 tig0001334:-73037 Msa1336960:CDS
ACCAAGTTGATCAGCTCCAA+AGG 0.526037 tig0001334:+74515 None:intergenic
AACTTATCTAACTGATGTTG+TGG 0.535055 tig0001334:-76632 Msa1336960:CDS
GTGTCTGCTACCAAATGTAA+AGG 0.535911 tig0001334:-73371 Msa1336960:CDS
TCATTTGTCGACCGATAGCA+AGG 0.538648 tig0001334:+73026 None:intergenic
GTGATGCAAAGGATGGGAAT+CGG 0.543695 tig0001334:-73103 Msa1336960:CDS
CGCTCAGCTGATAACACGAC+AGG 0.545338 tig0001334:-73140 Msa1336960:CDS
TATAAACGTGATGCAAAGGA+TGG 0.549790 tig0001334:-73110 Msa1336960:CDS
CAACAGCACTAGCAAGATCA+TGG 0.549961 tig0001334:-74632 Msa1336960:CDS
ATAGAGAGGTTTGCATTCAA+AGG 0.550568 tig0001334:-76673 Msa1336960:CDS
TGTTATAGCACTTTGGAGTG+TGG 0.558276 tig0001334:+73213 None:intergenic
TATTTAATCTCATTAGGCCA+AGG 0.562331 tig0001334:-74561 Msa1336960:CDS
ACACATAGAATTTACCTATG+AGG 0.562483 tig0001334:+77294 None:intergenic
CGTGTTATCAGCTGAGCGAA+CGG 0.563262 tig0001334:+73145 None:intergenic
ATAGCACTTTGGAGTGTGGC+CGG 0.564600 tig0001334:+73217 None:intergenic
AAGGTATAAACGTGATGCAA+AGG 0.571082 tig0001334:-73114 Msa1336960:CDS
ATGACAATGAAGAGAAACAT+AGG 0.572423 tig0001334:-73259 Msa1336960:CDS
GTTCAAAAGGAACACCAAGT+TGG 0.579133 tig0001334:-72772 Msa1336960:CDS
GCACTTTGGAGTGTGGCCGG+TGG 0.580031 tig0001334:+73220 None:intergenic
GAAAGAACCATCGAAATCGC+AGG 0.584602 tig0001334:+74327 None:intergenic
TCCGAAGAATATGAGAACAA+TGG 0.585411 tig0001334:-72876 Msa1336960:CDS
GGTCGACAAATGAGAAGCGA+AGG 0.590088 tig0001334:-73016 Msa1336960:CDS
TTCAAATTTCAAGATCCCAC+CGG 0.597703 tig0001334:-73236 Msa1336960:CDS
TATCTTCTTATAATGGCTGG+TGG 0.599636 tig0001334:-77353 Msa1336960:exon
GCAAAGGATGGGAATCGGAA+TGG 0.602918 tig0001334:-73098 Msa1336960:CDS
CAGTCATTGCAGGTATAGAG+AGG 0.605527 tig0001334:-76687 Msa1336960:CDS
GTATCATCTGTGTTACTACA+AGG 0.608964 tig0001334:+74477 None:intergenic
AAAACCAAAACATGATCATG+TGG 0.611772 tig0001334:-77451 Msa1336960:five_prime_UTR
ATAAACGTGATGCAAAGGAT+GGG 0.622044 tig0001334:-73109 Msa1336960:CDS
GAGATGGATTATATCCACCT+TGG 0.625994 tig0001334:+74544 None:intergenic
ATATCATCAGAAAACCAACT+TGG 0.626924 tig0001334:+72758 None:intergenic
GCAATAGGTGCCACCAACAA+TGG 0.628514 tig0001334:+76547 None:intergenic
TTAATCTCATTAGGCCAAGG+TGG 0.638847 tig0001334:-74558 Msa1336960:CDS
TTGGTAGAGTAATTTCACAA+TGG 0.651611 tig0001334:+74175 None:intergenic
CATAACATAGAGAAAAGATG+AGG 0.677023 tig0001334:+76489 None:intergenic
AATCATAGCGATGATTATCG+CGG 0.703876 tig0001334:-73068 Msa1336960:CDS
CTGGTGGACAGAAAACCAAG+AGG 0.752478 tig0001334:-77337 Msa1336960:CDS
ATACCAAGAAGAATGTACTG+TGG 0.754580 tig0001334:+72950 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAACTTTATAGAAATATATT+TGG - tig0001334:73676-73695 Msa1336960:intron 10.0%
!!! TTTTTTAATCAAAATTTAGA+TGG + tig0001334:74422-74441 None:intergenic 10.0%
!! ATAACATAAAAAATAATAAG+TGG + tig0001334:74702-74721 None:intergenic 10.0%
!! AAATTACTATTTATAGTTTA+TGG - tig0001334:75987-76006 Msa1336960:intron 10.0%
!! AATTACTATTTATAGTTTAT+GGG - tig0001334:75988-76007 Msa1336960:intron 10.0%
!! TAGAATATCATAAATCAAAA+GGG + tig0001334:73309-73328 None:intergenic 15.0%
!!! ATTTATTTTTTCCTTGTTTT+TGG - tig0001334:73351-73370 Msa1336960:CDS 15.0%
!! TGTAAATATGTAAATACAAA+AGG + tig0001334:73644-73663 None:intergenic 15.0%
!! AAAAATAAATGGAATAATCT+TGG + tig0001334:74113-74132 None:intergenic 15.0%
!! GTATATACTATATATAAAGT+AGG + tig0001334:74218-74237 None:intergenic 15.0%
!!! TGAAATTTTTATAATCATAG+AGG + tig0001334:74650-74669 None:intergenic 15.0%
!! ACATAATTTAGTGTAATTAT+AGG - tig0001334:75053-75072 Msa1336960:intron 15.0%
!!! TTTTTTAGAATGTATAGAAA+CGG + tig0001334:75273-75292 None:intergenic 15.0%
!! GCTATATTAATATGATATAA+TGG + tig0001334:77529-77548 None:intergenic 15.0%
!!! TTAAGGTACTATACAAATAT+TGG + tig0001334:72601-72620 None:intergenic 20.0%
!!! ATCATGTTTTGGTTTTTTTA+AGG + tig0001334:72618-72637 None:intergenic 20.0%
!!! TTTCAAGTTTCAAACTTATT+AGG - tig0001334:72841-72860 Msa1336960:CDS 20.0%
!!! TTCAAGTTTCAAACTTATTA+GGG - tig0001334:72842-72861 Msa1336960:CDS 20.0%
!! TTAGAGAAAAATATCACAAA+TGG + tig0001334:72933-72952 None:intergenic 20.0%
!! AGCAAATTTCATATTATCAA+TGG + tig0001334:72988-73007 None:intergenic 20.0%
!! ATGCAAGAATAATAGAATAT+TGG + tig0001334:73285-73304 None:intergenic 20.0%
!! GTAGAATATCATAAATCAAA+AGG + tig0001334:73310-73329 None:intergenic 20.0%
!! AATATGTAAATACAAAAGGA+GGG + tig0001334:73640-73659 None:intergenic 20.0%
!! AAATATGTAAATACAAAAGG+AGG + tig0001334:73641-73660 None:intergenic 20.0%
!!! TGATATTTTGTACACATATT+TGG - tig0001334:73810-73829 Msa1336960:intron 20.0%
!! CTATAGTATTTGTATAAAGT+AGG + tig0001334:74028-74047 None:intergenic 20.0%
!! AATACTATAGTATTAGCATT+AGG - tig0001334:74038-74057 Msa1336960:intron 20.0%
!! CTATACTATAACAAACTTAA+TGG + tig0001334:74068-74087 None:intergenic 20.0%
!! CGTAGACAATAATAATAAAT+TGG + tig0001334:74490-74509 None:intergenic 20.0%
!! AACAGTATAGTTAATAGATA+AGG - tig0001334:74616-74635 Msa1336960:CDS 20.0%
!!! TATTATATCCTTTTAGCATA+TGG + tig0001334:74736-74755 None:intergenic 20.0%
!! TGGTTTCATATCTATATAAT+TGG + tig0001334:74799-74818 None:intergenic 20.0%
!!! GTTTCTATACATTCTAAAAA+AGG - tig0001334:75272-75291 Msa1336960:intron 20.0%
!! AATGACTTAGATGATTAAAA+AGG + tig0001334:76037-76056 None:intergenic 20.0%
!!! ATTTATTTTTCTTGTGAAAC+AGG - tig0001334:76605-76624 Msa1336960:CDS 20.0%
!! TTAATCATAACCTTTACATT+TGG + tig0001334:76715-76734 None:intergenic 20.0%
!!! TAATTTTATCACAAAGTTTG+TGG - tig0001334:77494-77513 Msa1336960:five_prime_UTR 20.0%
!!! TTTTGATTCTCTCTTAAGTT+TGG - tig0001334:72663-72682 Msa1336960:CDS 25.0%
!!! TTCATTTTTCTAGAAGCATT+TGG - tig0001334:72697-72716 Msa1336960:CDS 25.0%
! CATTTGGTATCTTCTTATAA+TGG - tig0001334:72713-72732 Msa1336960:CDS 25.0%
! TCATATTATCAATGGAATTG+AGG + tig0001334:72980-72999 None:intergenic 25.0%
!!! ATAGGAAACTAGTCTTTAAT+AGG - tig0001334:73018-73037 Msa1336960:CDS 25.0%
!!! TAGGAAACTAGTCTTTAATA+GGG - tig0001334:73019-73038 Msa1336960:CDS 25.0%
!! ATTTTCGTCTTCCATAATAT+CGG - tig0001334:73045-73064 Msa1336960:CDS 25.0%
! AATTCAAATCCATTCAATTG+CGG + tig0001334:73110-73129 None:intergenic 25.0%
!! TATTGGTTGAATTATGACTA+TGG + tig0001334:73268-73287 None:intergenic 25.0%
! TAGATAAGTTACCAAATTTG+AGG + tig0001334:73431-73450 None:intergenic 25.0%
! GAAGTATATATATTTGTGGA+AGG + tig0001334:73618-73637 None:intergenic 25.0%
! TGTACAAAATATCAGTTTAG+TGG + tig0001334:73804-73823 None:intergenic 25.0%
! CATATATGCTTAAATATGAG+TGG + tig0001334:74296-74315 None:intergenic 25.0%
!! AATTGTTCATTGATTGAGAA+GGG + tig0001334:74358-74377 None:intergenic 25.0%
!! AAATTGTTCATTGATTGAGA+AGG + tig0001334:74359-74378 None:intergenic 25.0%
!! ACAACTTTTATGTGTATGTT+GGG + tig0001334:74585-74604 None:intergenic 25.0%
!! ACTCAAAATTCTCTTTGTTT+GGG + tig0001334:74852-74871 None:intergenic 25.0%
! AACTCAAAATTCTCTTTGTT+TGG + tig0001334:74853-74872 None:intergenic 25.0%
!!! AAACAAAGAGAATTTTGAGT+TGG - tig0001334:74852-74871 Msa1336960:intron 25.0%
! GATAAGGTTAAAACAACTTA+TGG - tig0001334:75235-75254 Msa1336960:intron 25.0%
! TCTCTCTATTTAATCTCATT+AGG - tig0001334:75506-75525 Msa1336960:intron 25.0%
!!! AAAAAGCACTTTTCTTTCAA+TGG - tig0001334:75630-75649 Msa1336960:intron 25.0%
! AATTAGGTTAAACAATATGG+CGG + tig0001334:76396-76415 None:intergenic 25.0%
! CAGAATTAGGTTAAACAATA+TGG + tig0001334:76399-76418 None:intergenic 25.0%
!! TGTTTAACCTAATTCTGAAA+AGG - tig0001334:76402-76421 Msa1336960:intron 25.0%
! AAGCATTAAGGTCATAAATA+AGG - tig0001334:76663-76682 Msa1336960:CDS 25.0%
! ATAAAAACTCTGTCGTATAA+CGG + tig0001334:76907-76926 None:intergenic 25.0%
!! TATATCTGATATTTTCACAG+TGG - tig0001334:77143-77162 Msa1336960:intron 25.0%
!!! GGTATGCAAGAATTTTTTTA+CGG - tig0001334:77168-77187 Msa1336960:intron 25.0%
AAAACCAAAACATGATCATG+TGG - tig0001334:72622-72641 Msa1336960:exon 30.0%
TCTCTCTTAAGTTTGGATAT+TGG - tig0001334:72670-72689 Msa1336960:CDS 30.0%
ACACATAGAATTTACCTATG+AGG + tig0001334:72782-72801 None:intergenic 30.0%
! TTTGAGTTAAAGCATATGCT+TGG - tig0001334:72897-72916 Msa1336960:CDS 30.0%
!! AATTTGCTGCTGAAAAACAT+AGG - tig0001334:73000-73019 Msa1336960:CDS 30.0%
CTGTAGTTGAATTTAACTGT+AGG - tig0001334:73158-73177 Msa1336960:CDS 30.0%
AGATTGATGCAAGTTCAATA+GGG + tig0001334:73194-73213 None:intergenic 30.0%
TTCAAATAGCACCAAAAACA+AGG + tig0001334:73365-73384 None:intergenic 30.0%
AACTTATCTAACTGATGTTG+TGG - tig0001334:73441-73460 Msa1336960:CDS 30.0%
AGCAAAGATGGTTAATAGTT+GGG - tig0001334:73483-73502 Msa1336960:intron 30.0%
CATAACATAGAGAAAAGATG+AGG + tig0001334:73587-73606 None:intergenic 30.0%
! CTCATCTTTTCTCTATGTTA+TGG - tig0001334:73585-73604 Msa1336960:intron 30.0%
! GAGGGAAGTATATATATTTG+TGG + tig0001334:73622-73641 None:intergenic 30.0%
GAGTTTAAATCTGTCAAAGA+CGG + tig0001334:73750-73769 None:intergenic 30.0%
TCTTCAATGAATAATACACG+TGG - tig0001334:73986-74005 Msa1336960:intron 30.0%
AGAGATCCATTAATCTTTGT+TGG - tig0001334:74256-74275 Msa1336960:CDS 30.0%
! ATTGTTCATTGATTGAGAAG+GGG + tig0001334:74357-74376 None:intergenic 30.0%
! GACAACTTTTATGTGTATGT+TGG + tig0001334:74586-74605 None:intergenic 30.0%
ATTAAGCACCATATGCTAAA+AGG - tig0001334:74725-74744 Msa1336960:intron 30.0%
AGATCATGACATCATAATCA+TGG + tig0001334:74819-74838 None:intergenic 30.0%
CCTCAAATTATTATCTGCAT+CGG + tig0001334:75028-75047 None:intergenic 30.0%
TTATCATAAGCTAATCCTTG+AGG - tig0001334:75203-75222 Msa1336960:intron 30.0%
TATCATAAGCTAATCCTTGA+GGG - tig0001334:75204-75223 Msa1336960:intron 30.0%
! CTATACATTCTAAAAAAGGC+TGG - tig0001334:75276-75295 Msa1336960:intron 30.0%
CAGTAACTTTCTGTTTGAAT+TGG + tig0001334:75336-75355 None:intergenic 30.0%
! TTCTTGTGTTTTCTTCCTAT+AGG - tig0001334:75400-75419 Msa1336960:intron 30.0%
! TCTTGTGTTTTCTTCCTATA+GGG - tig0001334:75401-75420 Msa1336960:intron 30.0%
TATTTAATCTCATTAGGCCA+AGG - tig0001334:75512-75531 Msa1336960:intron 30.0%
! TTCTTTCAATGGTGGTATTT+TGG - tig0001334:75641-75660 Msa1336960:intron 30.0%
! TCTATCTTGATTTTCTCTAG+TGG - tig0001334:75767-75786 Msa1336960:intron 30.0%
TTGGTAGAGTAATTTCACAA+TGG + tig0001334:75901-75920 None:intergenic 30.0%
! ACATTTTCAGACTTGTCATT+TGG + tig0001334:75920-75939 None:intergenic 30.0%
GTACACGTAATGAAACTTAA+AGG + tig0001334:76084-76103 None:intergenic 30.0%
AAGAGATACACATATGAGAT+GGG + tig0001334:76221-76240 None:intergenic 30.0%
CGTTAAGCATAATTCTGAAT+CGG + tig0001334:76246-76265 None:intergenic 30.0%
AAAAAATGTGCAGAGAATAG+AGG - tig0001334:76276-76295 Msa1336960:intron 30.0%
! ATCTTGTCCTTTTCAGAATT+AGG + tig0001334:76412-76431 None:intergenic 30.0%
ACACATATGAGATGAGAAAT+CGG - tig0001334:76433-76452 Msa1336960:intron 30.0%
ATTCTCTTACCAAAAAACAC+AGG + tig0001334:76514-76533 None:intergenic 30.0%
GTAAGAGAATGTGTGAAAAA+TGG - tig0001334:76524-76543 Msa1336960:CDS 30.0%
TGATTAAGCTATTGCCTATA+TGG - tig0001334:76728-76747 Msa1336960:intron 30.0%
! TGTTTTGTGAAAAATGTTGC+AGG + tig0001334:76790-76809 None:intergenic 30.0%
! GCAACATTTTTCACAAAACA+AGG - tig0001334:76790-76809 Msa1336960:intron 30.0%
ATGACAATGAAGAGAAACAT+AGG - tig0001334:76814-76833 Msa1336960:intron 30.0%
TAACGGCATTAACAGTATTA+TGG + tig0001334:76890-76909 None:intergenic 30.0%
! TAGTACCAATAAGCATTTTC+TGG - tig0001334:77094-77113 Msa1336960:intron 30.0%
AACAACCAGAAAATGCTTAT+TGG + tig0001334:77102-77121 None:intergenic 30.0%
! GCATTGTATACAAGTGTTTT+CGG - tig0001334:77225-77244 Msa1336960:intron 30.0%
GAAGTATACACAAGTTCAAA+AGG - tig0001334:77288-77307 Msa1336960:intron 30.0%
ATATCATCAGAAAACCAACT+TGG + tig0001334:77318-77337 None:intergenic 30.0%
!!! AAGTTGGTTTTCTGATGATA+TGG - tig0001334:77317-77336 Msa1336960:CDS 30.0%
CACGTTTGGATAATTACTAT+TGG - tig0001334:77346-77365 Msa1336960:exon 30.0%
CTCAAAGTTGTAACAGAGTT+AGG - tig0001334:72540-72559 Msa1336960:three_prime_UTR 35.0%
TGTAACAGAGTTAGGACTAT+TGG - tig0001334:72548-72567 Msa1336960:three_prime_UTR 35.0%
! GTTTCCACATGATCATGTTT+TGG + tig0001334:72629-72648 None:intergenic 35.0%
TGGTATCTTCTTATAATGGC+TGG - tig0001334:72717-72736 Msa1336960:CDS 35.0%
TATCTTCTTATAATGGCTGG+TGG - tig0001334:72720-72739 Msa1336960:CDS 35.0%
AATTCATGCATTCTCCTCAT+AGG - tig0001334:72765-72784 Msa1336960:CDS 35.0%
AATGGAATTGAGGCTTAAGA+AGG + tig0001334:72970-72989 None:intergenic 35.0%
TAATGTCGAAGCCGATATTA+TGG + tig0001334:73059-73078 None:intergenic 35.0%
!! TAATATCGGCTTCGACATTA+TGG - tig0001334:73059-73078 Msa1336960:CDS 35.0%
ATCATCATACCGCAATTGAA+TGG - tig0001334:73098-73117 Msa1336960:CDS 35.0%
TTGTAGTTGAATTCAACTGC+AGG + tig0001334:73134-73153 None:intergenic 35.0%
AATTTAACTGTAGGCAATCG+TGG - tig0001334:73167-73186 Msa1336960:CDS 35.0%
GAGATTGATGCAAGTTCAAT+AGG + tig0001334:73195-73214 None:intergenic 35.0%
! CTATTTGAAACAGTCATTGC+AGG - tig0001334:73376-73395 Msa1336960:CDS 35.0%
ATAGAGAGGTTTGCATTCAA+AGG - tig0001334:73400-73419 Msa1336960:CDS 35.0%
CAGCAAAGATGGTTAATAGT+TGG - tig0001334:73482-73501 Msa1336960:intron 35.0%
AAGATGGTTAATAGTTGGGT+TGG - tig0001334:73487-73506 Msa1336960:intron 35.0%
ACCTATTGCTGATGCTTATT+GGG - tig0001334:73540-73559 Msa1336960:intron 35.0%
GAGAAAAGATGAGGACATTA+TGG + tig0001334:73578-73597 None:intergenic 35.0%
GATTTAAACTCTGTCATTCG+AGG - tig0001334:73759-73778 Msa1336960:intron 35.0%
TCTGCAATGACTAATATACG+TGG - tig0001334:74176-74195 Msa1336960:CDS 35.0%
CAGACTCCAACAAAGATTAA+TGG + tig0001334:74265-74284 None:intergenic 35.0%
!!! CTACTGTTTTTGAAGAGTTC+AGG - tig0001334:74329-74348 Msa1336960:CDS 35.0%
GTGAAATGTAGAATCTGACT+TGG + tig0001334:74391-74410 None:intergenic 35.0%
TGTGTATGTTGGGATCAATT+TGG + tig0001334:74575-74594 None:intergenic 35.0%
ACTTCACTTCATTCTAGTGA+GGG + tig0001334:74770-74789 None:intergenic 35.0%
AACTTCACTTCATTCTAGTG+AGG + tig0001334:74771-74790 None:intergenic 35.0%
!! CTTTTTGATGTAGCTTGCAT+AGG + tig0001334:74910-74929 None:intergenic 35.0%
CCGATGCAGATAATAATTTG+AGG - tig0001334:75025-75044 Msa1336960:intron 35.0%
! TGTAATTATAGGTGTCGTGT+CGG - tig0001334:75064-75083 Msa1336960:intron 35.0%
CTTATCTTTATAAGCCCTCA+AGG + tig0001334:75221-75240 None:intergenic 35.0%
CTTGAGGGCTTATAAAGATA+AGG - tig0001334:75219-75238 Msa1336960:intron 35.0%
GAATGTATAGAAACGGCTTA+CGG + tig0001334:75266-75285 None:intergenic 35.0%
! GTATCATCTGTGTTACTACA+AGG + tig0001334:75599-75618 None:intergenic 35.0%
! AAGCACTTTTCTTTCAATGG+TGG - tig0001334:75633-75652 Msa1336960:intron 35.0%
ACATGAATGTTACACCCATT+AGG + tig0001334:75682-75701 None:intergenic 35.0%
TCTTACATCCAAGACACATT+TGG - tig0001334:75701-75720 Msa1336960:intron 35.0%
ACACATTTGGTTGGGTAATA+GGG - tig0001334:75714-75733 Msa1336960:intron 35.0%
CTCTTAAACATGTTCGTGAA+AGG + tig0001334:75851-75870 None:intergenic 35.0%
TATCTGTTATGTTACTGACG+TGG - tig0001334:76148-76167 Msa1336960:intron 35.0%
TACACATATGAGATGGGAAA+TGG + tig0001334:76215-76234 None:intergenic 35.0%
CAAGAGATACACATATGAGA+TGG + tig0001334:76222-76241 None:intergenic 35.0%
!!! GTAACAAGACCTGTGTTTTT+TGG - tig0001334:76502-76521 Msa1336960:CDS 35.0%
GTCATTGTAAGTCTCATAAC+GGG + tig0001334:76559-76578 None:intergenic 35.0%
GGTCATTGTAAGTCTCATAA+CGG + tig0001334:76560-76579 None:intergenic 35.0%
!! CGAAAACTGTGAAAGCATTA+AGG - tig0001334:76651-76670 Msa1336960:CDS 35.0%
!! ATGAGAAGCATTGTCCATAT+AGG + tig0001334:76745-76764 None:intergenic 35.0%
TGAAAAATGTTGCAGGTTGT+TGG + tig0001334:76783-76802 None:intergenic 35.0%
TTCAAATTTCAAGATCCCAC+CGG - tig0001334:76837-76856 Msa1336960:intron 35.0%
TGGACAATGTTATAGCACTT+TGG + tig0001334:76870-76889 None:intergenic 35.0%
AAGGTATAAACGTGATGCAA+AGG - tig0001334:76959-76978 Msa1336960:intron 35.0%
TATAAACGTGATGCAAAGGA+TGG - tig0001334:76963-76982 Msa1336960:intron 35.0%
ATAAACGTGATGCAAAGGAT+GGG - tig0001334:76964-76983 Msa1336960:intron 35.0%
AATCATAGCGATGATTATCG+CGG - tig0001334:77005-77024 Msa1336960:intron 35.0%
TTACCACAGTACATTCTTCT+TGG - tig0001334:77120-77139 Msa1336960:intron 35.0%
ATACCAAGAAGAATGTACTG+TGG + tig0001334:77126-77145 None:intergenic 35.0%
! TCTGATATTTTCACAGTGGT+TGG - tig0001334:77147-77166 Msa1336960:intron 35.0%
TCCGAAGAATATGAGAACAA+TGG - tig0001334:77197-77216 Msa1336960:intron 35.0%
CCCATTGTTCTCATATTCTT+CGG + tig0001334:77201-77220 None:intergenic 35.0%
CCGAAGAATATGAGAACAAT+GGG - tig0001334:77198-77217 Msa1336960:intron 35.0%
! TATACAAGTGTTTTCGGAGT+TGG - tig0001334:77231-77250 Msa1336960:intron 35.0%
!! ATTACTATTGGCTTCTAGCT+TGG - tig0001334:77358-77377 Msa1336960:exon 35.0%
TTGCTCATGATTGAGCACAA+TGG - tig0001334:72872-72891 Msa1336960:CDS 40.0%
CATGATATTGAGCAGAATCG+CGG + tig0001334:73224-73243 None:intergenic 40.0%
! CAAAGGTGTAGCCTCAAATT+TGG - tig0001334:73417-73436 Msa1336960:CDS 40.0%
CACTTCCATAATGCCATTGT+TGG - tig0001334:73513-73532 Msa1336960:intron 40.0%
TTCCATAATGCCATTGTTGG+TGG - tig0001334:73516-73535 Msa1336960:intron 40.0%
CACCTATTGCTGATGCTTAT+TGG - tig0001334:73539-73558 Msa1336960:intron 40.0%
CAATGAATAATACACGTGGC+TGG - tig0001334:73990-74009 Msa1336960:intron 40.0%
CAATGACTAATATACGTGGC+TGG - tig0001334:74180-74199 Msa1336960:CDS 40.0%
! TTGATGTAGCTTGCATAGGA+AGG + tig0001334:74906-74925 None:intergenic 40.0%
!! ATGAAGCACTGACATAGACA+CGG - tig0001334:74988-75007 Msa1336960:intron 40.0%
TATGAAGCATAGACACTCCT+CGG + tig0001334:75132-75151 None:intergenic 40.0%
TTAATCTCATTAGGCCAAGG+TGG - tig0001334:75515-75534 Msa1336960:intron 40.0%
!! GAGATGGATTATATCCACCT+TGG + tig0001334:75532-75551 None:intergenic 40.0%
AATCCATCTCTACAAGCCTT+TGG - tig0001334:75542-75561 Msa1336960:intron 40.0%
!!! TGGTATTTTGGTGTTTGTGC+TGG - tig0001334:75653-75672 Msa1336960:intron 40.0%
ACATCCAAGACACATTTGGT+TGG - tig0001334:75705-75724 Msa1336960:intron 40.0%
CATCCAAGACACATTTGGTT+GGG - tig0001334:75706-75725 Msa1336960:intron 40.0%
TTACCCAACCAAATGTGTCT+TGG + tig0001334:75712-75731 None:intergenic 40.0%
GACACATTTGGTTGGGTAAT+AGG - tig0001334:75713-75732 Msa1336960:intron 40.0%
GATGGGAAATGGATACTCAT+CGG + tig0001334:76204-76223 None:intergenic 40.0%
GATGAGAAATCGGTATGCAT+AGG - tig0001334:76443-76462 Msa1336960:intron 40.0%
CATAGGATAAGTCACCAACT+CGG - tig0001334:76460-76479 Msa1336960:intron 40.0%
TTCTGTAAGCGCTTCACTAT+AGG + tig0001334:76581-76600 None:intergenic 40.0%
GTGTCTGCTACCAAATGTAA+AGG - tig0001334:76702-76721 Msa1336960:intron 40.0%
!! TGTTATAGCACTTTGGAGTG+TGG + tig0001334:76863-76882 None:intergenic 40.0%
CTGATAACACGACAGGAAAA+AGG - tig0001334:76940-76959 Msa1336960:intron 40.0%
! GAAATGAAACGCCTTGCTAT+CGG - tig0001334:77036-77055 Msa1336960:intron 40.0%
GTTCAAAAGGAACACCAAGT+TGG - tig0001334:77301-77320 Msa1336960:intron 40.0%
! GTTTTCTGATGATATGGTCG+AGG - tig0001334:77323-77342 Msa1336960:CDS 40.0%
CAGTCATTGCAGGTATAGAG+AGG - tig0001334:73386-73405 Msa1336960:CDS 45.0%
CAACTCATCTGCAGCAAAGA+TGG - tig0001334:73471-73490 Msa1336960:intron 45.0%
TGCCACCAACAATGGCATTA+TGG + tig0001334:73521-73540 None:intergenic 45.0%
GCCCAATAAGCATCAGCAAT+AGG + tig0001334:73544-73563 None:intergenic 45.0%
TCAATGCTGCAAGTCCCTAT+AGG + tig0001334:75418-75437 None:intergenic 45.0%
CAACAGCACTAGCAAGATCA+TGG - tig0001334:75441-75460 Msa1336960:intron 45.0%
!! ACCAAGTTGATCAGCTCCAA+AGG + tig0001334:75561-75580 None:intergenic 45.0%
TGTTTGTGCTGGAAGCCTAA+TGG - tig0001334:75664-75683 Msa1336960:intron 45.0%
GTTTGTGCTGGAAGCCTAAT+GGG - tig0001334:75665-75684 Msa1336960:intron 45.0%
TGCTATACCTGCGATTTCGA+TGG - tig0001334:75739-75758 Msa1336960:intron 45.0%
GAAAGAACCATCGAAATCGC+AGG + tig0001334:75749-75768 None:intergenic 45.0%
GTTATGTTACTGACGTGGCA+TGG - tig0001334:76153-76172 Msa1336960:intron 45.0%
TGCAGAGAATAGAGGAATCG+AGG - tig0001334:76284-76303 Msa1336960:intron 45.0%
GCAGAGAATAGAGGAATCGA+GGG - tig0001334:76285-76304 Msa1336960:intron 45.0%
CCAACGTACGAAAATCTCAG+AGG + tig0001334:76317-76336 None:intergenic 45.0%
!!! CCTCTGAGATTTTCGTACGT+TGG - tig0001334:76314-76333 Msa1336960:intron 45.0%
GTTGGATGACGATTACCTCT+AGG - tig0001334:76332-76351 Msa1336960:intron 45.0%
GAATATGCTGTGTAGCCTAG+AGG + tig0001334:76350-76369 None:intergenic 45.0%
TGACGTGATAGTAACCGAGT+TGG + tig0001334:76477-76496 None:intergenic 45.0%
! TCACAGTTTTCGCGACAAAG+TGG + tig0001334:76643-76662 None:intergenic 45.0%
GTGATGCAAAGGATGGGAAT+CGG - tig0001334:76970-76989 Msa1336960:intron 45.0%
TCATTTGTCGACCGATAGCA+AGG + tig0001334:77050-77069 None:intergenic 45.0%
!! TTATAAAAAGAAAAAATAAA+TGG + tig0001334:74124-74143 None:intergenic 5.0%
! CTGGTGGACAGAAAACCAAG+AGG - tig0001334:72736-72755 Msa1336960:CDS 50.0%
GCATGAATTGCTGAGCCTCT+TGG + tig0001334:72754-72773 None:intergenic 50.0%
! GCAATAGGTGCCACCAACAA+TGG + tig0001334:73529-73548 None:intergenic 50.0%
!! ATGAAGCACTGACTCAGACG+CGG + tig0001334:74533-74552 None:intergenic 50.0%
ACATAGACACGGACACGACA+TGG - tig0001334:74999-75018 Msa1336960:intron 50.0%
GCATAGACACTCCTCGGATT+AGG + tig0001334:75126-75145 None:intergenic 50.0%
GCTCCAAAGGCTTGTAGAGA+TGG + tig0001334:75548-75567 None:intergenic 50.0%
!! GCCTTTGGAGCTGATCAACT+TGG - tig0001334:75557-75576 Msa1336960:intron 50.0%
TTACTGACGTGGCATGGACA+TGG - tig0001334:76159-76178 Msa1336960:intron 50.0%
TACTGACGTGGCATGGACAT+GGG - tig0001334:76160-76179 Msa1336960:intron 50.0%
AACAGTGAGCGAGAGAGAAG+AGG + tig0001334:76372-76391 None:intergenic 50.0%
!! ATAGCACTTTGGAGTGTGGC+CGG + tig0001334:76859-76878 None:intergenic 50.0%
CGTGTTATCAGCTGAGCGAA+CGG + tig0001334:76931-76950 None:intergenic 50.0%
GCAAAGGATGGGAATCGGAA+TGG - tig0001334:76975-76994 Msa1336960:intron 50.0%
GGTCGACAAATGAGAAGCGA+AGG - tig0001334:77057-77076 Msa1336960:intron 50.0%
TGATATGGTCGAGGCACGTT+TGG - tig0001334:77332-77351 Msa1336960:CDS 50.0%
GATTAGGTGTGCCCCAGTGT+CGG + tig0001334:75110-75129 None:intergenic 55.0%
CGCTCAGCTGATAACACGAC+AGG - tig0001334:76933-76952 Msa1336960:intron 55.0%
ACTGGGGCACACCTAATCCG+AGG - tig0001334:75112-75131 Msa1336960:intron 60.0%
!! CACTTTGGAGTGTGGCCGGT+GGG + tig0001334:76855-76874 None:intergenic 60.0%
ACCGACGCGTGTCCGACACT+GGG - tig0001334:75095-75114 Msa1336960:intron 65.0%
!! GCACTTTGGAGTGTGGCCGG+TGG + tig0001334:76856-76875 None:intergenic 65.0%
CACCGACGCGTGTCCGACAC+TGG - tig0001334:75094-75113 Msa1336960:intron 70.0%
! CCCCAGTGTCGGACACGCGT+CGG + tig0001334:75099-75118 None:intergenic 70.0%
CCGACGCGTGTCCGACACTG+GGG - tig0001334:75096-75115 Msa1336960:intron 70.0%
Chromosome Type Strat End Strand Name
tig0001334 gene 72532 77563 72532 ID=Msa1336960;Name=Msa1336960
tig0001334 mRNA 72532 77563 72532 ID=Msa1336960-mRNA-1;Parent=Msa1336960;Name=Msa1336960-mRNA-1;_AED=0.04;_eAED=0.04;_QI=200|1|1|1|1|1|4|108|542
tig0001334 exon 72532 73468 72532 ID=Msa1336960-mRNA-1:exon:935;Parent=Msa1336960-mRNA-1
tig0001334 exon 74147 74673 74147 ID=Msa1336960-mRNA-1:exon:934;Parent=Msa1336960-mRNA-1
tig0001334 exon 76489 76706 76489 ID=Msa1336960-mRNA-1:exon:933;Parent=Msa1336960-mRNA-1
tig0001334 exon 77309 77563 77309 ID=Msa1336960-mRNA-1:exon:932;Parent=Msa1336960-mRNA-1
tig0001334 five_prime_UTR 77364 77563 77364 ID=Msa1336960-mRNA-1:five_prime_utr;Parent=Msa1336960-mRNA-1
tig0001334 CDS 77309 77363 77309 ID=Msa1336960-mRNA-1:cds;Parent=Msa1336960-mRNA-1
tig0001334 CDS 76489 76706 76489 ID=Msa1336960-mRNA-1:cds;Parent=Msa1336960-mRNA-1
tig0001334 CDS 74147 74673 74147 ID=Msa1336960-mRNA-1:cds;Parent=Msa1336960-mRNA-1
tig0001334 CDS 72640 73468 72640 ID=Msa1336960-mRNA-1:cds;Parent=Msa1336960-mRNA-1
tig0001334 three_prime_UTR 72532 72639 72532 ID=Msa1336960-mRNA-1:three_prime_utr;Parent=Msa1336960-mRNA-1
Gene Sequence

>Msa1336960

ATGGCTGGTGGACAGAAAACCAAGAGGCTCAGCAATTCATGCATTCTCCTCATAGTCATTGCAGGTATAGAGAGGTTTGCATTCAAAGGTGTAGCCTCAAATTTGGTAACTTATCTAACTGATGTTGTGGACTTAAGCAACTCATCTGCAGCAAAGATGGTTAATAGTTGGGTTGGATTCACTTCCATAATGCCATTGTTGGTGGCACCTATTGCTGATGCTTATTGGGCTAAATATTCAACCATAATGTCCTCATCTTTTCTCTATGTTATGGGACTTGCAGCATTGACAACAACAGCACTAGCAAGATCATGGCATCACAAAAACAGATCAATGTCATCTTCATTTCTTTCATTATCTCTCTATTTAATCTCATTAGGCCAAGGTGGATATAATCCATCTCTACAAGCCTTTGGAGCTGATCAACTTGGTGAAGATGAAGAACTACCTTGTAGTAACACAGATGATACAAGTAGCAACAAAAAAGCACTTTTCTTTCAATGGTGGTATTTTGGTGTTTGTGCTGGAAGCCTAATGGGTGTAACATTCATGTCTTACATCCAAGACACATTTGGTTGGGTAATAGGGTTTGCTATACCTGCGATTTCGATGGTTCTTTCTATCTTGATTTTCTCTAGTGGAAGCTCTATATATTCGTATAAACAACAAGATGATGATGATGATCTTCGAGATAAGAAGCCTTTCACGAACATGTTTAAGAGTATAAAAGAATCTGCATTGAAATGCTTCCATTGTGAAATTACTCTACCAAATGACAAGTCTGAAAATGTCGAACTAGAGCTTCAAGAGAGACCACTTTGTCGCGAAAACTGTGAAAGCATTAAGGTCATAAATAAGGATTCTAAAATCAGCATGTGTCTGCTACCAAATGTAAAGGTTATGATTAAGCTATTGCCTATATGGACAATGCTTCTCATGTTTGCAGTGATTTTCCAACAACCTGCAACATTTTTCACAAAACAAGGCATGACAATGAAGAGAAACATAGGTTCAAATTTCAAGATCCCACCGGCCACACTCCAAAGTGCTATAACATTGTCCATAATACTGTTAATGCCGTTATACGACAGAGTTTTTATACCGTTCGCTCAGCTGATAACACGACAGGAAAAAGGTATAAACGTGATGCAAAGGATGGGAATCGGAATGGTACTCTCAATCATAGCGATGATTATCGCGGCACTAGTTGAAATGAAACGCCTTGCTATCGGTCGACAAATGAGAAGCGAAGGATTACTATCAGAAATAGTACCAATAAGCATTTTCTGGTTGTTACCACAGTACATTCTTCTTGGTATATCTGATATTTTCACAGTGGTTGGTATGCAAGAATTTTTTTACGGTGAAGTTCCGAAGAATATGAGAACAATGGGAATTGCATTGTATACAAGTGTTTTCGGAGTTGGAAGCTTTGTTAGTGCATTGATGATTACACTTGTTGAAGTATACACAAGTTCAAAAGGAACACCAAGTTGGTTTTCTGATGATATGGTCGAGGCACGTTTGGATAATTACTATTGGCTTCTAGCTTGGTTAAGTTCAATTAGCTTATTGTTGTATACACTATTGTGTAAATATTACTATAATAAGAGTGAATCAGATAGTTAA
Protein sequence

>Msa1336960

MAGGQKTKRLSNSCILLIVIAGIERFAFKGVASNLVTYLTDVVDLSNSSAAKMVNSWVGFTSIMPLLVAPIADAYWAKYSTIMSSSFLYVMGLAALTTTALARSWHHKNRSMSSSFLSLSLYLISLGQGGYNPSLQAFGADQLGEDEELPCSNTDDTSSNKKALFFQWWYFGVCAGSLMGVTFMSYIQDTFGWVIGFAIPAISMVLSILIFSSGSSIYSYKQQDDDDDLRDKKPFTNMFKSIKESALKCFHCEITLPNDKSENVELELQERPLCRENCESIKVINKDSKISMCLLPNVKVMIKLLPIWTMLLMFAVIFQQPATFFTKQGMTMKRNIGSNFKIPPATLQSAITLSIILLMPLYDRVFIPFAQLITRQEKGINVMQRMGIGMVLSIIAMIIAALVEMKRLAIGRQMRSEGLLSEIVPISIFWLLPQYILLGISDIFTVVGMQEFFYGEVPKNMRTMGIALYTSVFGVGSFVSALMITLVEVYTSSKGTPSWFSDDMVEARLDNYYWLLAWLSSISLLLYTLLCKYYYNKSESDS*