AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1342730

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1342730 MtrunA17_Chr1g0191711 94.343 548 30 1 1 547 1 548 0.0 1061
Msa1342730 MtrunA17_Chr4g0050021 37.138 587 278 10 43 543 29 610 2.18e-127 387
Msa1342730 MtrunA17_Chr2g0279481 34.958 595 283 12 47 542 33 622 2.66e-123 377
Msa1342730 MtrunA17_Chr8g0382231 34.829 557 317 9 29 546 24 573 1.27e-119 366
Msa1342730 MtrunA17_Chr2g0303541 33.201 503 277 8 42 487 10 510 1.27e-94 301
Msa1342730 MtrunA17_Chr2g0320691 28.673 565 337 8 48 547 13 576 4.66e-86 279
Msa1342730 MtrunA17_Chr4g0010091 33.673 490 281 9 41 491 18 502 7.44e-84 273
Msa1342730 MtrunA17_Chr4g0053841 30.597 536 317 9 47 540 2 524 1.20e-82 269
Msa1342730 MtrunA17_Chr5g0401451 31.927 545 313 14 44 546 26 554 1.84e-82 269
Msa1342730 MtrunA17_Chr2g0285511 30.943 530 282 10 42 509 10 517 6.67e-82 267
Msa1342730 MtrunA17_Chr1g0207461 29.356 528 302 8 59 525 29 546 1.51e-81 267
Msa1342730 MtrunA17_Chr1g0181181 30.642 483 292 9 42 487 10 486 3.54e-81 264
Msa1342730 MtrunA17_Chr7g0245201 32.258 527 299 14 56 529 59 580 5.37e-81 266
Msa1342730 MtrunA17_Chr3g0108331 29.693 522 327 9 34 527 1 510 1.40e-78 257
Msa1342730 MtrunA17_Chr1g0150191 31.838 468 264 8 59 479 28 487 8.93e-78 256
Msa1342730 MtrunA17_Chr8g0374011 28.765 591 327 14 42 541 4 591 2.01e-76 254
Msa1342730 MtrunA17_Chr5g0432861 28.653 527 326 11 44 541 12 517 4.03e-76 251
Msa1342730 MtrunA17_Chr4g0076521 31.416 452 273 9 59 478 27 473 5.29e-75 249
Msa1342730 MtrunA17_Chr5g0401341 28.733 529 304 9 59 525 28 545 9.86e-73 243
Msa1342730 MtrunA17_Chr8g0367891 32.252 493 262 14 61 501 29 501 2.99e-71 239
Msa1342730 MtrunA17_Chr1g0195911 30.271 479 278 10 59 491 37 505 1.95e-70 237
Msa1342730 MtrunA17_Chr1g0199631 29.870 462 270 7 76 487 8 465 5.98e-68 229
Msa1342730 MtrunA17_Chr7g0268781 28.108 555 310 11 59 535 37 580 1.56e-67 229
Msa1342730 MtrunA17_Chr2g0290741 30.067 449 269 8 61 478 29 463 3.23e-63 217
Msa1342730 MtrunA17_Chr5g0412591 28.820 517 257 13 59 501 27 506 1.00e-60 211
Msa1342730 MtrunA17_Chr4g0003891 30.435 345 211 8 209 527 35 376 1.32e-49 177
Msa1342730 MtrunA17_Chr2g0332281 28.947 342 215 8 209 527 13 349 2.59e-47 170
Msa1342730 MtrunA17_Chr4g0037661 25.510 490 304 9 91 531 39 516 1.81e-43 162
Msa1342730 MtrunA17_Chr4g0023801 26.512 430 271 11 67 466 14 428 4.25e-37 144
Msa1342730 MtrunA17_Chr1g0163311 24.164 538 331 16 57 539 2 517 4.08e-30 124
Msa1342730 MtrunA17_Chr2g0280541 26.016 369 231 8 128 466 2 358 4.58e-29 119
Msa1342730 MtrunA17_Chr1g0206621 36.232 138 87 1 65 202 75 211 3.86e-24 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1342730 AT5G64330.1 32.672 655 289 11 40 547 34 683 3.17e-119 369
Msa1342730 AT5G64330.2 34.872 585 240 10 40 488 34 613 1.14e-118 365
Msa1342730 AT5G10250.2 32.616 558 328 9 24 541 16 565 1.86e-109 340
Msa1342730 AT5G64330.3 34.657 554 221 10 71 488 6 554 9.75e-107 332
Msa1342730 AT5G10250.1 33.455 550 319 10 24 541 16 550 1.14e-106 332
Msa1342730 AT3G08660.1 32.816 515 288 12 59 530 35 534 2.15e-88 284
Msa1342730 AT5G03250.1 31.928 498 277 7 42 481 10 503 2.46e-88 284
Msa1342730 AT1G30440.1 31.361 507 286 5 42 487 10 515 4.32e-87 283
Msa1342730 AT2G30520.1 29.868 529 338 10 35 541 8 525 4.65e-84 273
Msa1342730 AT3G08660.3 32.568 479 269 11 95 530 7 474 1.57e-81 264
Msa1342730 AT5G67385.1 31.579 475 279 10 40 478 8 472 3.17e-80 263
Msa1342730 AT3G08570.1 31.191 529 298 11 59 529 35 555 2.00e-79 261
Msa1342730 AT3G44820.1 27.700 574 335 9 47 543 12 582 3.97e-79 261
Msa1342730 AT5G13600.1 31.846 493 273 12 49 487 17 500 6.44e-79 259
Msa1342730 AT5G67440.3 31.356 472 289 8 59 498 28 496 2.26e-78 258
Msa1342730 AT5G67440.1 31.356 472 289 8 59 498 28 496 2.26e-78 258
Msa1342730 AT5G48800.1 31.837 490 276 9 41 491 25 495 9.86e-78 257
Msa1342730 AT2G30520.2 29.630 486 310 9 78 541 2 477 1.66e-74 246
Msa1342730 AT2G30520.3 29.630 486 310 9 78 541 2 477 1.66e-74 246
Msa1342730 AT2G14820.2 30.357 504 289 9 43 495 11 503 6.21e-72 242
Msa1342730 AT2G14820.1 30.357 504 289 9 43 495 11 503 6.21e-72 242
Msa1342730 AT2G47860.1 28.788 528 294 10 60 518 39 553 7.59e-71 239
Msa1342730 AT5G67385.4 31.881 436 252 9 79 478 1 427 1.29e-70 236
Msa1342730 AT5G67385.5 31.881 436 252 9 79 478 1 427 1.29e-70 236
Msa1342730 AT5G67385.2 31.881 436 252 9 79 478 1 427 1.29e-70 236
Msa1342730 AT5G67385.3 31.881 436 252 9 79 478 1 427 1.29e-70 236
Msa1342730 AT5G67440.2 30.217 460 286 8 71 498 3 459 1.48e-70 236
Msa1342730 AT1G67900.5 30.980 510 271 10 59 499 27 524 6.07e-70 236
Msa1342730 AT1G67900.1 30.980 510 271 10 59 499 27 524 6.07e-70 236
Msa1342730 AT1G67900.3 30.980 510 271 10 59 499 27 524 6.07e-70 236
Msa1342730 AT1G67900.6 30.980 510 271 10 59 499 27 524 6.07e-70 236
Msa1342730 AT1G67900.2 30.980 510 271 10 59 499 27 524 6.07e-70 236
Msa1342730 AT1G67900.4 30.980 510 271 10 59 499 27 524 6.07e-70 236
Msa1342730 AT5G66560.1 28.598 542 294 10 48 498 9 548 4.06e-69 235
Msa1342730 AT1G03010.2 29.668 482 280 9 59 488 10 484 6.09e-69 233
Msa1342730 AT4G31820.1 29.769 477 272 9 53 488 22 476 8.68e-69 232
Msa1342730 AT1G03010.1 29.668 482 280 9 59 488 37 511 1.18e-68 233
Msa1342730 AT1G03010.3 29.668 482 280 9 59 488 36 510 1.44e-68 233
Msa1342730 AT5G47800.3 28.689 488 309 10 58 512 26 507 1.82e-67 228
Msa1342730 AT5G47800.1 28.689 488 309 10 58 512 26 507 1.82e-67 228
Msa1342730 AT5G47800.4 28.689 488 309 10 58 512 26 507 1.82e-67 228
Msa1342730 AT3G49970.1 33.415 410 248 10 70 470 6 399 2.71e-67 226
Msa1342730 AT3G49970.2 33.415 410 248 10 70 470 6 399 2.71e-67 226
Msa1342730 AT3G08660.2 32.663 398 228 10 59 431 35 417 1.17e-66 222
Msa1342730 AT4G37590.1 29.314 481 293 10 43 491 11 476 1.88e-66 226
Msa1342730 AT5G47800.2 28.689 488 309 10 58 512 139 620 2.24e-66 228
Msa1342730 AT3G50840.1 28.791 521 335 11 48 540 5 517 5.77e-66 224
Msa1342730 AT2G47860.3 27.586 551 294 11 60 518 39 576 9.94e-66 226
Msa1342730 AT2G23050.1 31.293 441 248 9 67 479 36 449 1.57e-64 218
Msa1342730 AT3G26490.1 28.431 510 282 10 60 499 28 524 9.26e-64 219
Msa1342730 AT3G26490.3 28.431 510 282 10 60 499 28 524 9.26e-64 219
Msa1342730 AT3G26490.4 28.431 510 282 10 60 499 28 524 9.26e-64 219
Msa1342730 AT5G66560.3 28.269 520 280 10 70 498 2 519 1.18e-63 220
Msa1342730 AT5G66560.4 30.493 446 258 8 103 498 66 509 4.69e-63 218
Msa1342730 AT4G31820.2 29.213 445 252 9 85 488 1 423 1.32e-59 206
Msa1342730 AT2G47860.4 29.426 418 234 8 60 429 39 443 2.60e-57 198
Msa1342730 AT3G50840.2 28.387 465 300 8 103 540 23 481 6.84e-57 199
Msa1342730 AT3G50840.3 28.387 465 300 8 103 540 23 481 6.84e-57 199
Msa1342730 AT5G66560.2 28.118 473 247 10 48 429 9 479 6.60e-54 190
Msa1342730 AT5G03250.2 28.934 394 219 6 146 481 1 391 1.77e-53 189
Msa1342730 AT3G26490.2 29.158 463 245 10 60 450 28 479 2.23e-53 189
Msa1342730 AT2G47860.6 27.891 441 234 9 60 429 39 466 1.83e-52 186
Msa1342730 AT1G52770.1 31.129 363 214 8 218 546 26 386 2.69e-49 177
Msa1342730 AT3G15570.1 30.058 346 200 8 220 527 29 370 2.69e-48 174
Msa1342730 AT5G48130.1 26.371 474 281 12 45 480 26 469 5.26e-47 174
Msa1342730 AT1G50280.1 27.511 458 285 10 57 470 2 456 2.15e-46 170
Msa1342730 AT1G50280.2 28.293 410 250 8 102 470 17 423 5.70e-46 169
Msa1342730 AT3G19850.1 27.338 417 241 8 103 470 52 455 9.14e-45 166
Msa1342730 AT2G47860.5 26.018 442 251 9 146 518 1 435 3.52e-44 164
Msa1342730 AT2G47860.2 26.018 442 251 9 146 518 1 435 3.52e-44 164
Msa1342730 AT2G23050.2 30.000 360 199 8 146 479 1 333 7.67e-43 157
Msa1342730 AT5G17580.2 27.273 462 289 10 56 486 18 463 3.34e-37 145
Msa1342730 AT5G17580.1 27.273 462 289 10 56 486 3 448 3.57e-37 145
Msa1342730 AT3G22104.1 24.714 437 276 11 60 465 6 420 1.76e-34 137
Msa1342730 AT3G03510.1 26.738 374 248 8 115 481 8 362 3.24e-34 135
Msa1342730 AT3G22104.4 24.242 363 226 8 132 465 3 345 3.96e-25 108
Msa1342730 AT3G22104.2 24.242 363 226 8 132 465 3 345 3.96e-25 108
Msa1342730 AT3G22104.3 24.242 363 226 8 132 465 3 345 3.96e-25 108

Find 74 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCTGGAAGATTGTCTATT+TGG 0.174553 tig0006014:-9909 None:intergenic
CTATCTTCTTCTGTCAAATA+TGG 0.209690 tig0006014:-11392 None:intergenic
CATTTCTAATGTTATCTTCA+TGG 0.244593 tig0006014:+10091 Msa1342730:CDS
TGCATAACTTCAAATGCTTT+TGG 0.249335 tig0006014:-11712 None:intergenic
GTGTTACAATCTTATGTTCT+TGG 0.259144 tig0006014:+10681 Msa1342730:CDS
TCAACTTTGATTTCAAGTTT+TGG 0.279449 tig0006014:-10225 None:intergenic
TAGGTTCCTGTATAGAAAAT+TGG 0.294506 tig0006014:+10346 Msa1342730:CDS
TGTGAGAAATGATTCTGTTT+TGG 0.294906 tig0006014:-10070 None:intergenic
TAGAAAATTGGACTACAAAA+TGG 0.300863 tig0006014:+10358 Msa1342730:CDS
TGATGCTTGTCTTACTCCTT+TGG 0.322877 tig0006014:-11574 None:intergenic
ACATCATACCTGCCTTTAAC+AGG 0.328556 tig0006014:-10521 None:intergenic
GCGCTTCTTGTGACAATCTA+TGG 0.350078 tig0006014:-11442 None:intergenic
CAAGAAGTGAATATTTGAAT+AGG 0.369741 tig0006014:+9839 Msa1342730:CDS
ATCCAAATAGACAATCTTCC+AGG 0.378879 tig0006014:+9907 Msa1342730:CDS
TTTAAGGAATCAGCAACTAT+AGG 0.385762 tig0006014:-9282 None:intergenic
TGAATAGGCTTGTCTTCAAA+AGG 0.388500 tig0006014:+9854 Msa1342730:CDS
ATGTGTTTATGTGTCACATT+AGG 0.404324 tig0006014:-10408 None:intergenic
ACATGTTCACTTGTTCTAGA+AGG 0.404709 tig0006014:-11520 None:intergenic
AGCTATTGACATGTACCTTA+AGG 0.420374 tig0006014:+10866 Msa1342730:CDS
TTGTAACTCCTTGGCCCAAT+GGG 0.422828 tig0006014:-10154 None:intergenic
TTGTGAATCAATTTCCCATT+GGG 0.436436 tig0006014:+10140 Msa1342730:CDS
TTACTTGATTCAGGTTTGCC+AGG 0.439410 tig0006014:+9425 Msa1342730:intron
AGAAAATGTGTGTAATGTAT+TGG 0.445599 tig0006014:+11415 Msa1342730:CDS
AAATCCTGTTGATAAGTTCA+TGG 0.445770 tig0006014:-11495 None:intergenic
TTCCAGCTTTCACTTGCATA+AGG 0.448370 tig0006014:+9486 Msa1342730:CDS
AAATCAAAGTTGATGCTTGT+TGG 0.453295 tig0006014:+10235 Msa1342730:CDS
CACCTTATGCAAGTGAAAGC+TGG 0.456130 tig0006014:-9488 None:intergenic
GAATAGGCTTGTCTTCAAAA+GGG 0.459367 tig0006014:+9855 Msa1342730:CDS
GATATCTTGGTTAAGAATCA+AGG 0.460464 tig0006014:+10618 Msa1342730:CDS
TTGCCCATGAACTTATCAAC+AGG 0.463591 tig0006014:+11491 Msa1342730:CDS
AAAGGAGTAAGACAAGCATC+AGG 0.466683 tig0006014:+11576 Msa1342730:CDS
GTGTCGTGTTCAAGATATCT+TGG 0.467443 tig0006014:+10605 Msa1342730:CDS
GCTGCAAAAGTTCACAATGT+TGG 0.475366 tig0006014:+10720 Msa1342730:CDS
ACAATATAATGAAGCTATGT+TGG 0.478289 tig0006014:-9995 None:intergenic
TTTCACTAATTGTAACTCCT+TGG 0.480054 tig0006014:-10163 None:intergenic
TTGTCAAATTCTGTTACGGT+TGG 0.488790 tig0006014:+9953 Msa1342730:CDS
ATCAATTTCCCATTGGGCCA+AGG 0.494419 tig0006014:+10146 Msa1342730:CDS
AGTTCACAATGTTGGAAGGT+TGG 0.507286 tig0006014:+10728 Msa1342730:CDS
TTACTTCACCTGTTAAAGGC+AGG 0.511487 tig0006014:+10513 Msa1342730:CDS
GTTGCTCAGAAGCAATTGCT+TGG 0.515310 tig0006014:+10187 Msa1342730:CDS
TTAGTTGTCAAATTCTGTTA+CGG 0.519205 tig0006014:+9949 Msa1342730:CDS
ATCATTAGCAGAACTTGCCC+TGG 0.519372 tig0006014:-9443 None:intergenic
TAGTTGCTGATTCCTTAAAA+CGG 0.524321 tig0006014:+9286 Msa1342730:CDS
GTTGTGAATCAATTTCCCAT+TGG 0.525573 tig0006014:+10139 Msa1342730:CDS
ATTGTAACTCCTTGGCCCAA+TGG 0.529828 tig0006014:-10155 None:intergenic
AATGATTTAATCATACAAGT+TGG 0.533319 tig0006014:+9460 Msa1342730:CDS
AATCCTGTTGATAAGTTCAT+GGG 0.538863 tig0006014:-11494 None:intergenic
GGAATGAAATCGGAGTTTGT+AGG 0.540139 tig0006014:+10327 Msa1342730:CDS
GTTATCTATCACAAGTTGCA+AGG 0.555039 tig0006014:+10757 Msa1342730:CDS
GCAACTATAGGAACATTAGC+TGG 0.557389 tig0006014:-9270 None:intergenic
CAAAAGTTCACAATGTTGGA+AGG 0.563172 tig0006014:+10724 Msa1342730:CDS
TAGAACAAGTGAACATGTCA+TGG 0.564910 tig0006014:+11525 Msa1342730:CDS
GATGATGAAGTCACAACTGT+TGG 0.567585 tig0006014:+11664 Msa1342730:CDS
TCACAATGTTGGAAGGTTGG+TGG 0.570684 tig0006014:+10731 Msa1342730:CDS
GTTACGCTTTCCTCCACTCA+AGG 0.570870 tig0006014:-9232 None:intergenic
TCAAAGTTGATGCTTGTTGG+TGG 0.571613 tig0006014:+10238 Msa1342730:CDS
AATAGGCTTGTCTTCAAAAG+GGG 0.571861 tig0006014:+9856 Msa1342730:CDS
TACTTGATTCAGGTTTGCCA+GGG 0.586307 tig0006014:+9426 Msa1342730:intron
ATATTCACTTCTTGAGACCA+TGG 0.588159 tig0006014:-9830 None:intergenic
TGGTTCTCTAAAGTCACACT+TGG 0.598171 tig0006014:+10378 Msa1342730:CDS
TCATGGACAATGGCAAGTAG+TGG 0.603006 tig0006014:+11542 Msa1342730:CDS
ATGAGTGAAGATATTGAACA+AGG 0.613707 tig0006014:+10036 Msa1342730:CDS
GACTGTGAAAATGCCTTGAG+TGG 0.613781 tig0006014:+9219 Msa1342730:CDS
AGTGAACATGTCATGGACAA+TGG 0.618994 tig0006014:+11532 Msa1342730:CDS
TTATCTATCACAAGTTGCAA+GGG 0.626529 tig0006014:+10758 Msa1342730:CDS
ATGTGTGCTAGAGAGAAGAG+TGG 0.638803 tig0006014:+10566 Msa1342730:CDS
GTAGTGGATCAAATTACCAA+AGG 0.640603 tig0006014:+11558 Msa1342730:CDS
TAATGTGACACATAAACACA+TGG 0.648727 tig0006014:+10410 Msa1342730:CDS
AAGGAGTAAGACAAGCATCA+GGG 0.655380 tig0006014:+11577 Msa1342730:CDS
AATGTTGGAAGGTTGGTGGA+TGG 0.656204 tig0006014:+10735 Msa1342730:CDS
TGTGAAAATGCCTTGAGTGG+AGG 0.661907 tig0006014:+9222 Msa1342730:CDS
CAAATAGACAATCTTCCAGG+TGG 0.702112 tig0006014:+9910 Msa1342730:CDS
TAGCTGGTAGAACAACACAT+CGG 0.769094 tig0006014:-9254 None:intergenic
AAGGGATCAGAAGCTCACAG+TGG 0.808993 tig0006014:+10776 Msa1342730:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATATATATCTTATAATAAA+AGG - tig0006014:9516-9535 None:intergenic 10.0%
!!! TTTTAGTTATATCTACTTAT+TGG + tig0006014:9627-9646 Msa1342730:intron 15.0%
!! ATAATATTAGTATAACCTTA+AGG - tig0006014:10884-10903 None:intergenic 15.0%
!! TTAAAACTTCAATAAAATCA+CGG - tig0006014:11125-11144 None:intergenic 15.0%
!! AATGATTTAATCATACAAGT+TGG + tig0006014:9460-9479 Msa1342730:CDS 20.0%
!! ATTAAGTTATATATCACCAT+TGG + tig0006014:9597-9616 Msa1342730:intron 20.0%
!! GTAGATATAACTAAAAGTAA+TGG - tig0006014:9623-9642 None:intergenic 20.0%
!! TGTTATATAACTAAAAGCAA+TGG - tig0006014:9653-9672 None:intergenic 20.0%
!!! TATAGCTTTTGTAAAAATCA+TGG + tig0006014:11147-11166 Msa1342730:intron 20.0%
!!! TAAATTTAACTCAATTTTGC+AGG + tig0006014:11364-11383 Msa1342730:intron 20.0%
!!! GTTTTGATTTTTCCGTTTTA+AGG - tig0006014:9301-9320 None:intergenic 25.0%
! TAAAACGGAAAAATCAAAAC+TGG + tig0006014:9301-9320 Msa1342730:CDS 25.0%
! GTTTCAATAATGACATGTTT+GGG - tig0006014:9343-9362 None:intergenic 25.0%
! TGTTTCAATAATGACATGTT+TGG - tig0006014:9344-9363 None:intergenic 25.0%
! CAAGAAGTGAATATTTGAAT+AGG + tig0006014:9839-9858 Msa1342730:CDS 25.0%
! TTAGTTGTCAAATTCTGTTA+CGG + tig0006014:9949-9968 Msa1342730:CDS 25.0%
! ACAATATAATGAAGCTATGT+TGG - tig0006014:9998-10017 None:intergenic 25.0%
! CATTTCTAATGTTATCTTCA+TGG + tig0006014:10091-10110 Msa1342730:CDS 25.0%
!!! TCAACTTTGATTTCAAGTTT+TGG - tig0006014:10228-10247 None:intergenic 25.0%
! ATTCAATCTATCAAAAAACG+TGG + tig0006014:10306-10325 Msa1342730:CDS 25.0%
! TAGAAAATTGGACTACAAAA+TGG + tig0006014:10358-10377 Msa1342730:CDS 25.0%
!!! AAGCATTTTAATCAAACACT+CGG - tig0006014:10460-10479 None:intergenic 25.0%
!!! TTTTTTACTTCACCTGTTAA+AGG + tig0006014:10509-10528 Msa1342730:CDS 25.0%
! TCTTTGATATTGCATACAAT+CGG + tig0006014:10951-10970 Msa1342730:intron 25.0%
!!! TACTTCAAGTTTTATTTGTG+TGG + tig0006014:11250-11269 Msa1342730:intron 25.0%
! CATATGTCTCTATGATTATA+CGG + tig0006014:11322-11341 Msa1342730:intron 25.0%
! AGAAAATGTGTGTAATGTAT+TGG + tig0006014:11415-11434 Msa1342730:CDS 25.0%
!! ATGAACAAGTTTGAGTTTTC+TGG + tig0006014:9168-9187 Msa1342730:CDS 30.0%
! TTCACAGTCAAGAATTTTCA+TGG - tig0006014:9208-9227 None:intergenic 30.0%
TTTAAGGAATCAGCAACTAT+AGG - tig0006014:9285-9304 None:intergenic 30.0%
!! TAGTTGCTGATTCCTTAAAA+CGG + tig0006014:9286-9305 Msa1342730:CDS 30.0%
ATGTCTCATTTACTTGATTC+AGG + tig0006014:9416-9435 Msa1342730:intron 30.0%
TAACTAAAAGTAATGGCCAA+TGG - tig0006014:9616-9635 None:intergenic 30.0%
GCTATGATATGCTTGAAATT+AGG - tig0006014:9748-9767 None:intergenic 30.0%
ATGAGTGAAGATATTGAACA+AGG + tig0006014:10036-10055 Msa1342730:CDS 30.0%
! TGTGAGAAATGATTCTGTTT+TGG - tig0006014:10073-10092 None:intergenic 30.0%
TTGTGAATCAATTTCCCATT+GGG + tig0006014:10140-10159 Msa1342730:CDS 30.0%
TTTCACTAATTGTAACTCCT+TGG - tig0006014:10166-10185 None:intergenic 30.0%
!! AAATCAAAGTTGATGCTTGT+TGG + tig0006014:10235-10254 Msa1342730:CDS 30.0%
CAAAAAACGTGGAATGAAAT+CGG + tig0006014:10317-10336 Msa1342730:CDS 30.0%
TAGGTTCCTGTATAGAAAAT+TGG + tig0006014:10346-10365 Msa1342730:CDS 30.0%
! TGTAGTCCAATTTTCTATAC+AGG - tig0006014:10355-10374 None:intergenic 30.0%
ATGTGTTTATGTGTCACATT+AGG - tig0006014:10411-10430 None:intergenic 30.0%
TAATGTGACACATAAACACA+TGG + tig0006014:10410-10429 Msa1342730:CDS 30.0%
GATATCTTGGTTAAGAATCA+AGG + tig0006014:10618-10637 Msa1342730:CDS 30.0%
GTGTTACAATCTTATGTTCT+TGG + tig0006014:10681-10700 Msa1342730:CDS 30.0%
TTATCTATCACAAGTTGCAA+GGG + tig0006014:10758-10777 Msa1342730:CDS 30.0%
! TCACAATCTCTACAATTTTG+TGG - tig0006014:10831-10850 None:intergenic 30.0%
AATCACAGTCATATTCTAGT+TGG + tig0006014:10926-10945 Msa1342730:intron 30.0%
! ATCACAGTCATATTCTAGTT+GGG + tig0006014:10927-10946 Msa1342730:intron 30.0%
! GTTTGGTTCAACAATTAGTT+TGG + tig0006014:11083-11102 Msa1342730:intron 30.0%
! AGCTTTTGTAAAAATCATGG+TGG + tig0006014:11150-11169 Msa1342730:intron 30.0%
GAGACATATGCAAAACAATA+TGG - tig0006014:11312-11331 None:intergenic 30.0%
CTATCTTCTTCTGTCAAATA+TGG - tig0006014:11395-11414 None:intergenic 30.0%
AGCATGTGATGAAAAATGAT+AGG + tig0006014:11468-11487 Msa1342730:CDS 30.0%
! AATCCTGTTGATAAGTTCAT+GGG - tig0006014:11497-11516 None:intergenic 30.0%
! AAATCCTGTTGATAAGTTCA+TGG - tig0006014:11498-11517 None:intergenic 30.0%
! TGCATAACTTCAAATGCTTT+TGG - tig0006014:11715-11734 None:intergenic 30.0%
! TAGGAACATGAGGTACTTAT+TGG - tig0006014:9729-9748 None:intergenic 35.0%
TGCTTGAAATTAGGAACATG+AGG - tig0006014:9739-9758 None:intergenic 35.0%
ATATTCACTTCTTGAGACCA+TGG - tig0006014:9833-9852 None:intergenic 35.0%
TGAATAGGCTTGTCTTCAAA+AGG + tig0006014:9854-9873 Msa1342730:CDS 35.0%
GAATAGGCTTGTCTTCAAAA+GGG + tig0006014:9855-9874 Msa1342730:CDS 35.0%
AATAGGCTTGTCTTCAAAAG+GGG + tig0006014:9856-9875 Msa1342730:CDS 35.0%
ATCCAAATAGACAATCTTCC+AGG + tig0006014:9907-9926 Msa1342730:CDS 35.0%
! TCAAATGTTTTTCTACCACC+TGG - tig0006014:9928-9947 None:intergenic 35.0%
TTGTCAAATTCTGTTACGGT+TGG + tig0006014:9953-9972 Msa1342730:CDS 35.0%
GTTGTGAATCAATTTCCCAT+TGG + tig0006014:10139-10158 Msa1342730:CDS 35.0%
! GAGACAGAATTTTCTTCAGT+TGG - tig0006014:10483-10502 None:intergenic 35.0%
CAAAAGTTCACAATGTTGGA+AGG + tig0006014:10724-10743 Msa1342730:CDS 35.0%
GTTATCTATCACAAGTTGCA+AGG + tig0006014:10757-10776 Msa1342730:CDS 35.0%
AGCTATTGACATGTACCTTA+AGG + tig0006014:10866-10885 Msa1342730:CDS 35.0%
CCAAACATGCACTTAAAAAC+AGG - tig0006014:11069-11088 None:intergenic 35.0%
!!! CCTGTTTTTAAGTGCATGTT+TGG + tig0006014:11066-11085 Msa1342730:intron 35.0%
! ACATGTTCACTTGTTCTAGA+AGG - tig0006014:11523-11542 None:intergenic 35.0%
TAGAACAAGTGAACATGTCA+TGG + tig0006014:11525-11544 Msa1342730:CDS 35.0%
!! GTAGTGGATCAAATTACCAA+AGG + tig0006014:11558-11577 Msa1342730:CDS 35.0%
TAGCTGGTAGAACAACACAT+CGG - tig0006014:9257-9276 None:intergenic 40.0%
GCAACTATAGGAACATTAGC+TGG - tig0006014:9273-9292 None:intergenic 40.0%
! TTACTTGATTCAGGTTTGCC+AGG + tig0006014:9425-9444 Msa1342730:intron 40.0%
! TACTTGATTCAGGTTTGCCA+GGG + tig0006014:9426-9445 Msa1342730:intron 40.0%
TTCCAGCTTTCACTTGCATA+AGG + tig0006014:9486-9505 Msa1342730:CDS 40.0%
CACCTGGAAGATTGTCTATT+TGG - tig0006014:9912-9931 None:intergenic 40.0%
CAAATAGACAATCTTCCAGG+TGG + tig0006014:9910-9929 Msa1342730:CDS 40.0%
!! TCAAAGTTGATGCTTGTTGG+TGG + tig0006014:10238-10257 Msa1342730:CDS 40.0%
GGAATGAAATCGGAGTTTGT+AGG + tig0006014:10327-10346 Msa1342730:CDS 40.0%
!! TGGTTCTCTAAAGTCACACT+TGG + tig0006014:10378-10397 Msa1342730:CDS 40.0%
TTACTTCACCTGTTAAAGGC+AGG + tig0006014:10513-10532 Msa1342730:CDS 40.0%
ACATCATACCTGCCTTTAAC+AGG - tig0006014:10524-10543 None:intergenic 40.0%
!!! GAGAAGAGTGGTTTTGATGT+TGG + tig0006014:10578-10597 Msa1342730:CDS 40.0%
GTGTCGTGTTCAAGATATCT+TGG + tig0006014:10605-10624 Msa1342730:CDS 40.0%
GCTGCAAAAGTTCACAATGT+TGG + tig0006014:10720-10739 Msa1342730:CDS 40.0%
AGTTCACAATGTTGGAAGGT+TGG + tig0006014:10728-10747 Msa1342730:CDS 40.0%
TGCATACAATCGGTCAAATG+TGG + tig0006014:10961-10980 Msa1342730:intron 40.0%
TTGCCCATGAACTTATCAAC+AGG + tig0006014:11491-11510 Msa1342730:CDS 40.0%
AGTGAACATGTCATGGACAA+TGG + tig0006014:11532-11551 Msa1342730:CDS 40.0%
TGATGCTTGTCTTACTCCTT+TGG - tig0006014:11577-11596 None:intergenic 40.0%
AAAGGAGTAAGACAAGCATC+AGG + tig0006014:11576-11595 Msa1342730:CDS 40.0%
AAGGAGTAAGACAAGCATCA+GGG + tig0006014:11577-11596 Msa1342730:CDS 40.0%
GATGATGAAGTCACAACTGT+TGG + tig0006014:11664-11683 Msa1342730:CDS 40.0%
GACTGTGAAAATGCCTTGAG+TGG + tig0006014:9219-9238 Msa1342730:CDS 45.0%
TGTGAAAATGCCTTGAGTGG+AGG + tig0006014:9222-9241 Msa1342730:CDS 45.0%
ATCATTAGCAGAACTTGCCC+TGG - tig0006014:9446-9465 None:intergenic 45.0%
CACCTTATGCAAGTGAAAGC+TGG - tig0006014:9491-9510 None:intergenic 45.0%
CAGCTTTCACTTGCATAAGG+TGG + tig0006014:9489-9508 Msa1342730:intron 45.0%
!! GTTGATTTTCCAGCTTGCCA+TGG + tig0006014:9813-9832 Msa1342730:intron 45.0%
ATCAATTTCCCATTGGGCCA+AGG + tig0006014:10146-10165 Msa1342730:CDS 45.0%
TTGTAACTCCTTGGCCCAAT+GGG - tig0006014:10157-10176 None:intergenic 45.0%
ATTGTAACTCCTTGGCCCAA+TGG - tig0006014:10158-10177 None:intergenic 45.0%
GTTGCTCAGAAGCAATTGCT+TGG + tig0006014:10187-10206 Msa1342730:CDS 45.0%
ATGTGTGCTAGAGAGAAGAG+TGG + tig0006014:10566-10585 Msa1342730:CDS 45.0%
TCACAATGTTGGAAGGTTGG+TGG + tig0006014:10731-10750 Msa1342730:CDS 45.0%
!! AATGTTGGAAGGTTGGTGGA+TGG + tig0006014:10735-10754 Msa1342730:CDS 45.0%
! CAGGTTTGCGACTTTGACTT+GGG - tig0006014:11050-11069 None:intergenic 45.0%
ACAGGTTTGCGACTTTGACT+TGG - tig0006014:11051-11070 None:intergenic 45.0%
GCGCTTCTTGTGACAATCTA+TGG - tig0006014:11445-11464 None:intergenic 45.0%
TCATGGACAATGGCAAGTAG+TGG + tig0006014:11542-11561 Msa1342730:CDS 45.0%
GTTACGCTTTCCTCCACTCA+AGG - tig0006014:9235-9254 None:intergenic 50.0%
TTCTTGAGACCATGGCAAGC+TGG - tig0006014:9825-9844 None:intergenic 50.0%
AAGGGATCAGAAGCTCACAG+TGG + tig0006014:10776-10795 Msa1342730:CDS 50.0%
TGGCTGATCCACCTCAATCG+TGG + tig0006014:10981-11000 Msa1342730:intron 55.0%
ATCGTGGTCGTGCAGCAGTT+TGG + tig0006014:10997-11016 Msa1342730:intron 55.0%
CTGCACGACCACGATTGAGG+TGG - tig0006014:10992-11011 None:intergenic 60.0%
CTGCTGCACGACCACGATTG+AGG - tig0006014:10995-11014 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0006014 gene 9158 11742 9158 ID=Msa1342730;Name=Msa1342730
tig0006014 mRNA 9158 11742 9158 ID=Msa1342730-mRNA-1;Parent=Msa1342730;Name=Msa1342730-mRNA-1;_AED=0.01;_eAED=0.01;_QI=10|1|0.5|1|1|1|4|0|547
tig0006014 exon 9158 9322 9158 ID=Msa1342730-mRNA-1:exon:3715;Parent=Msa1342730-mRNA-1
tig0006014 exon 9438 9507 9438 ID=Msa1342730-mRNA-1:exon:3716;Parent=Msa1342730-mRNA-1
tig0006014 exon 9826 10887 9826 ID=Msa1342730-mRNA-1:exon:3717;Parent=Msa1342730-mRNA-1
tig0006014 exon 11386 11742 11386 ID=Msa1342730-mRNA-1:exon:3718;Parent=Msa1342730-mRNA-1
tig0006014 five_prime_UTR 9158 9167 9158 ID=Msa1342730-mRNA-1:five_prime_utr;Parent=Msa1342730-mRNA-1
tig0006014 CDS 9168 9322 9168 ID=Msa1342730-mRNA-1:cds;Parent=Msa1342730-mRNA-1
tig0006014 CDS 9438 9507 9438 ID=Msa1342730-mRNA-1:cds;Parent=Msa1342730-mRNA-1
tig0006014 CDS 9826 10887 9826 ID=Msa1342730-mRNA-1:cds;Parent=Msa1342730-mRNA-1
tig0006014 CDS 11386 11742 11386 ID=Msa1342730-mRNA-1:cds;Parent=Msa1342730-mRNA-1
Gene Sequence

>Msa1342730

ATGAACAAGTTTGAGTTTTCTGGTGTTGATAAAGCGACCATGAAAATTCTTGACTGTGAAAATGCCTTGAGTGGAGGAAAGCGTAACCGATGTGTTGTTCTACCAGCTAATGTTCCTATAGTTGCTGATTCCTTAAAACGGAAAAATCAAAACTGGTTTGCCAGGGCAAGTTCTGCTAATGATTTAATCATACAAGTTGGTGATTCCAGCTTTCACTTGCATAAGCTTGCCATGGTCTCAAGAAGTGAATATTTGAATAGGCTTGTCTTCAAAAGGGGAAGTAACATAGAAGACAATAATCATACTATCCAAATAGACAATCTTCCAGGTGGTAGAAAAACATTTGAGTTAGTTGTCAAATTCTGTTACGGTTGGAAATTTGATATGACATCATCCAACATAGCTTCATTATATTGTGCTGCAAATTTCTTAGAAATGAGTGAAGATATTGAACAAGGAAATCTAATTTCCAAAACAGAATCATTTCTCACATTTCTAATGTTATCTTCATGGAAAGACACATTTCGTATACTCAAAAGTTGTGAATCAATTTCCCATTGGGCCAAGGAGTTACAATTAGTGAAACGTTGCTCAGAAGCAATTGCTTGGAAAGCATGTGCAATTCCAAAACTTGAAATCAAAGTTGATGCTTGTTGGTGGTTTGAAGATGTATCACTTCTACGTATAGATCATTTTATTGAAGTGATTCAATCTATCAAAAAACGTGGAATGAAATCGGAGTTTGTAGGTTCCTGTATAGAAAATTGGACTACAAAATGGTTCTCTAAAGTCACACTTGGACTTGATCCTAATGTGACACATAAACACATGGCAATTCAGTTGCATAAAGTTTCAACCGAGTGTTTGATTAAAATGCTTCCAACTGAAGAAAATTCTGTCTCTTGCAATTTTTTACTTCACCTGTTAAAGGCAGGTATGATGTTGAAAATCAATCATGAGTTGTTATGTGTGCTAGAGAGAAGAGTGGTTTTGATGTTGGAGCAGTGTCGTGTTCAAGATATCTTGGTTAAGAATCAAGGAGATAAAGATTCTTTGTATGATGTTGATGTTGTTGTTAGAGTGTTACAATCTTATGTTCTTGGCATTTCAAGTAATTCTGCTGCAAAAGTTCACAATGTTGGAAGGTTGGTGGATGGTTATCTATCACAAGTTGCAAGGGATCAGAAGCTCACAGTGGAAAGTTTCAAATTGCTTGTTGAAGTATTGCCACAAAATTGTAGAGATTGTGATGACAATCTCTATAGAGCTATTGACATGTACCTTAAGGCACATCCATATTTGACAGAAGAAGATAGAGAAAATGTGTGTAATGTATTGGAATACCATAGATTGTCACAAGAAGCGCGTCAGCATGTGATGAAAAATGATAGGTTGCCCATGAACTTATCAACAGGATTTGTCCTTCTAGAACAAGTGAACATGTCATGGACAATGGCAAGTAGTGGATCAAATTACCAAAGGAGTAAGACAAGCATCAGGGTAAGCAAAGATTTTGAAAACAGACAGATGAATCAAGAGATAAAAGTAATGAGAAAAGATGTTGAGATGATGAAGTCACAACTGTTGGAACTAAATACTTGCAAAATGAAGCTCCAAAAGCATTTGAAGTTATGCACTCGTTGA
Protein sequence

>Msa1342730

MNKFEFSGVDKATMKILDCENALSGGKRNRCVVLPANVPIVADSLKRKNQNWFARASSANDLIIQVGDSSFHLHKLAMVSRSEYLNRLVFKRGSNIEDNNHTIQIDNLPGGRKTFELVVKFCYGWKFDMTSSNIASLYCAANFLEMSEDIEQGNLISKTESFLTFLMLSSWKDTFRILKSCESISHWAKELQLVKRCSEAIAWKACAIPKLEIKVDACWWFEDVSLLRIDHFIEVIQSIKKRGMKSEFVGSCIENWTTKWFSKVTLGLDPNVTHKHMAIQLHKVSTECLIKMLPTEENSVSCNFLLHLLKAGMMLKINHELLCVLERRVVLMLEQCRVQDILVKNQGDKDSLYDVDVVVRVLQSYVLGISSNSAAKVHNVGRLVDGYLSQVARDQKLTVESFKLLVEVLPQNCRDCDDNLYRAIDMYLKAHPYLTEEDRENVCNVLEYHRLSQEARQHVMKNDRLPMNLSTGFVLLEQVNMSWTMASSGSNYQRSKTSIRVSKDFENRQMNQEIKVMRKDVEMMKSQLLELNTCKMKLQKHLKLCTR*