AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0165740

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0165740 MtrunA17_Chr1g0191711 94.526 548 29 1 1 547 1 548 0.0 1066
Msa0165740 MtrunA17_Chr4g0050021 37.138 587 278 10 43 543 29 610 1.35e-127 388
Msa0165740 MtrunA17_Chr2g0279481 34.958 595 283 12 47 542 33 622 1.79e-123 378
Msa0165740 MtrunA17_Chr8g0382231 34.829 557 317 9 29 546 24 573 4.61e-119 365
Msa0165740 MtrunA17_Chr2g0303541 33.201 503 277 8 42 487 10 510 7.38e-95 302
Msa0165740 MtrunA17_Chr2g0320691 28.673 565 337 8 48 547 13 576 2.93e-86 279
Msa0165740 MtrunA17_Chr4g0010091 33.673 490 281 9 41 491 18 502 7.21e-84 273
Msa0165740 MtrunA17_Chr4g0053841 30.597 536 317 9 47 540 2 524 4.95e-83 270
Msa0165740 MtrunA17_Chr5g0401451 31.927 545 313 14 44 546 26 554 7.43e-83 270
Msa0165740 MtrunA17_Chr2g0285511 30.943 530 282 10 42 509 10 517 7.11e-82 267
Msa0165740 MtrunA17_Chr1g0207461 29.356 528 302 8 59 525 29 546 1.67e-81 266
Msa0165740 MtrunA17_Chr7g0245201 32.258 527 299 14 56 529 59 580 7.43e-81 265
Msa0165740 MtrunA17_Chr1g0181181 30.642 483 292 9 42 487 10 486 1.14e-80 263
Msa0165740 MtrunA17_Chr3g0108331 29.693 522 327 9 34 527 1 510 1.73e-78 257
Msa0165740 MtrunA17_Chr1g0150191 31.838 468 264 8 59 479 28 487 8.13e-78 256
Msa0165740 MtrunA17_Chr8g0374011 28.765 591 327 14 42 541 4 591 1.67e-76 254
Msa0165740 MtrunA17_Chr5g0432861 28.653 527 326 11 44 541 12 517 3.03e-76 251
Msa0165740 MtrunA17_Chr4g0076521 31.416 452 273 9 59 478 27 473 3.95e-75 249
Msa0165740 MtrunA17_Chr5g0401341 28.733 529 304 9 59 525 28 545 9.17e-73 244
Msa0165740 MtrunA17_Chr8g0367891 32.252 493 262 14 61 501 29 501 2.69e-71 239
Msa0165740 MtrunA17_Chr1g0195911 30.271 479 278 10 59 491 37 505 7.64e-71 238
Msa0165740 MtrunA17_Chr7g0268781 28.108 555 310 11 59 535 37 580 6.36e-68 231
Msa0165740 MtrunA17_Chr1g0199631 29.870 462 270 7 76 487 8 465 1.12e-67 229
Msa0165740 MtrunA17_Chr2g0290741 30.067 449 269 8 61 478 29 463 2.22e-63 217
Msa0165740 MtrunA17_Chr5g0412591 28.820 517 257 13 59 501 27 506 8.33e-61 211
Msa0165740 MtrunA17_Chr4g0003891 30.725 345 210 8 209 527 35 376 1.46e-51 182
Msa0165740 MtrunA17_Chr2g0332281 28.947 342 215 8 209 527 13 349 2.42e-47 170
Msa0165740 MtrunA17_Chr4g0037661 25.510 490 304 9 91 531 39 516 1.88e-43 162
Msa0165740 MtrunA17_Chr1g0163311 25.836 538 322 15 57 539 2 517 1.73e-39 150
Msa0165740 MtrunA17_Chr4g0023801 26.512 430 271 11 67 466 14 428 5.16e-37 144
Msa0165740 MtrunA17_Chr2g0280541 26.016 369 231 8 128 466 2 358 3.51e-29 120
Msa0165740 MtrunA17_Chr1g0206621 36.232 138 87 1 65 202 75 211 3.75e-24 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0165740 AT5G64330.1 32.672 655 289 11 40 547 34 683 2.42e-119 369
Msa0165740 AT5G64330.2 34.872 585 240 10 40 488 34 613 7.25e-119 365
Msa0165740 AT5G10250.2 32.975 558 326 10 24 541 16 565 4.43e-109 339
Msa0165740 AT5G64330.3 34.657 554 221 10 71 488 6 554 9.14e-107 332
Msa0165740 AT5G10250.1 33.455 550 319 10 24 541 16 550 2.74e-106 331
Msa0165740 AT5G03250.1 31.928 498 277 7 42 481 10 503 1.75e-88 285
Msa0165740 AT3G08660.1 32.816 515 288 12 59 530 35 534 1.76e-88 284
Msa0165740 AT1G30440.1 31.361 507 286 5 42 487 10 515 2.45e-87 283
Msa0165740 AT2G30520.1 29.868 529 338 10 35 541 8 525 5.01e-84 273
Msa0165740 AT3G08660.3 32.568 479 269 11 95 530 7 474 1.55e-81 264
Msa0165740 AT5G67385.1 31.579 475 279 10 40 478 8 472 1.78e-80 264
Msa0165740 AT3G08570.1 31.191 529 298 11 59 529 35 555 1.57e-79 261
Msa0165740 AT3G44820.1 27.700 574 335 9 47 543 12 582 3.54e-79 261
Msa0165740 AT5G13600.1 31.846 493 273 12 49 487 17 500 5.16e-79 259
Msa0165740 AT5G67440.3 31.356 472 289 8 59 498 28 496 5.03e-78 256
Msa0165740 AT5G67440.1 31.356 472 289 8 59 498 28 496 5.03e-78 256
Msa0165740 AT5G48800.1 31.837 490 276 9 41 491 25 495 6.15e-78 257
Msa0165740 AT2G30520.2 29.630 486 310 9 78 541 2 477 1.52e-74 246
Msa0165740 AT2G30520.3 29.630 486 310 9 78 541 2 477 1.52e-74 246
Msa0165740 AT2G14820.2 30.357 504 289 9 43 495 11 503 1.05e-71 241
Msa0165740 AT2G14820.1 30.357 504 289 9 43 495 11 503 1.05e-71 241
Msa0165740 AT2G47860.1 28.788 528 294 10 60 518 39 553 2.05e-71 240
Msa0165740 AT5G67385.4 31.881 436 252 9 79 478 1 427 5.77e-71 237
Msa0165740 AT5G67385.5 31.881 436 252 9 79 478 1 427 5.77e-71 237
Msa0165740 AT5G67385.2 31.881 436 252 9 79 478 1 427 5.77e-71 237
Msa0165740 AT5G67385.3 31.881 436 252 9 79 478 1 427 5.77e-71 237
Msa0165740 AT5G67440.2 30.217 460 286 8 71 498 3 459 2.27e-70 235
Msa0165740 AT1G67900.5 30.980 510 271 10 59 499 27 524 4.63e-70 237
Msa0165740 AT1G67900.1 30.980 510 271 10 59 499 27 524 4.63e-70 237
Msa0165740 AT1G67900.3 30.980 510 271 10 59 499 27 524 4.63e-70 237
Msa0165740 AT1G67900.6 30.980 510 271 10 59 499 27 524 4.63e-70 237
Msa0165740 AT1G67900.2 30.980 510 271 10 59 499 27 524 4.63e-70 237
Msa0165740 AT1G67900.4 30.980 510 271 10 59 499 27 524 4.63e-70 237
Msa0165740 AT1G03010.2 29.668 482 280 9 59 488 10 484 2.74e-69 234
Msa0165740 AT5G66560.1 28.598 542 294 10 48 498 9 548 2.98e-69 235
Msa0165740 AT1G03010.3 29.668 482 280 9 59 488 36 510 4.43e-69 234
Msa0165740 AT4G31820.1 29.769 477 272 9 53 488 22 476 6.15e-69 232
Msa0165740 AT1G03010.1 29.668 482 280 9 59 488 37 511 6.23e-69 234
Msa0165740 AT3G49970.1 33.415 410 248 10 70 470 6 399 1.15e-67 228
Msa0165740 AT3G49970.2 33.415 410 248 10 70 470 6 399 1.15e-67 228
Msa0165740 AT5G47800.3 28.689 488 309 10 58 512 26 507 1.37e-67 228
Msa0165740 AT5G47800.1 28.689 488 309 10 58 512 26 507 1.37e-67 228
Msa0165740 AT5G47800.4 28.689 488 309 10 58 512 26 507 1.37e-67 228
Msa0165740 AT3G08660.2 32.663 398 228 10 59 431 35 417 1.29e-66 222
Msa0165740 AT5G47800.2 28.689 488 309 10 58 512 139 620 2.13e-66 228
Msa0165740 AT4G37590.1 29.314 481 293 10 43 491 11 476 3.03e-66 225
Msa0165740 AT2G47860.3 27.586 551 294 11 60 518 39 576 3.82e-66 227
Msa0165740 AT3G50840.1 28.791 521 335 11 48 540 5 517 4.88e-66 224
Msa0165740 AT2G23050.1 31.293 441 248 9 67 479 36 449 1.37e-64 218
Msa0165740 AT3G26490.1 28.431 510 282 10 60 499 28 524 9.26e-64 219
Msa0165740 AT3G26490.3 28.431 510 282 10 60 499 28 524 9.26e-64 219
Msa0165740 AT3G26490.4 28.431 510 282 10 60 499 28 524 9.26e-64 219
Msa0165740 AT5G66560.3 28.269 520 280 10 70 498 2 519 9.80e-64 220
Msa0165740 AT5G66560.4 30.493 446 258 8 103 498 66 509 4.06e-63 218
Msa0165740 AT4G31820.2 29.213 445 252 9 85 488 1 423 1.01e-59 206
Msa0165740 AT2G47860.4 29.426 418 234 8 60 429 39 443 1.06e-57 199
Msa0165740 AT3G50840.2 28.387 465 300 8 103 540 23 481 6.11e-57 199
Msa0165740 AT3G50840.3 28.387 465 300 8 103 540 23 481 6.11e-57 199
Msa0165740 AT5G66560.2 28.118 473 247 10 48 429 9 479 4.51e-54 190
Msa0165740 AT5G03250.2 28.934 394 219 6 146 481 1 391 1.15e-53 189
Msa0165740 AT3G26490.2 29.158 463 245 10 60 450 28 479 1.69e-53 189
Msa0165740 AT2G47860.6 27.891 441 234 9 60 429 39 466 9.57e-53 186
Msa0165740 AT1G52770.1 31.129 363 214 8 218 546 26 386 2.38e-49 177
Msa0165740 AT3G15570.1 30.058 346 200 8 220 527 29 370 2.09e-48 174
Msa0165740 AT5G48130.1 27.176 471 281 14 45 480 26 469 1.66e-46 172
Msa0165740 AT1G50280.1 27.511 458 285 10 57 470 2 456 2.24e-46 170
Msa0165740 AT1G50280.2 28.293 410 250 8 102 470 17 423 4.80e-46 169
Msa0165740 AT3G19850.1 27.338 417 241 8 103 470 52 455 7.13e-45 167
Msa0165740 AT2G47860.5 26.018 442 251 9 146 518 1 435 1.33e-44 165
Msa0165740 AT2G47860.2 26.018 442 251 9 146 518 1 435 1.33e-44 165
Msa0165740 AT2G23050.2 30.000 360 199 8 146 479 1 333 4.81e-43 157
Msa0165740 AT5G17580.2 27.273 462 289 10 56 486 18 463 1.53e-36 143
Msa0165740 AT5G17580.1 27.273 462 289 10 56 486 3 448 1.99e-36 143
Msa0165740 AT3G22104.1 24.714 437 276 11 60 465 6 420 2.02e-34 136
Msa0165740 AT3G03510.1 26.385 379 253 8 115 486 8 367 6.60e-33 131
Msa0165740 AT3G22104.4 24.242 363 226 8 132 465 3 345 4.22e-25 108
Msa0165740 AT3G22104.2 24.242 363 226 8 132 465 3 345 4.22e-25 108
Msa0165740 AT3G22104.3 24.242 363 226 8 132 465 3 345 4.22e-25 108

Find 74 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCTGGAAGATTGTCTATT+TGG 0.174553 1_4:-46498358 None:intergenic
CTATCTTCTTCTGTCAAATA+TGG 0.209690 1_4:-46499840 None:intergenic
CATTTCTAATGTTATCTTCA+TGG 0.244593 1_4:+46498540 Msa0165740:CDS
TGCATAACTTCAAATGCTTT+TGG 0.249335 1_4:-46500160 None:intergenic
GTGTTACAATCTTATGTTCT+TGG 0.259144 1_4:+46499130 Msa0165740:CDS
TCAACTTTGATTTCAAGTTT+TGG 0.279449 1_4:-46498674 None:intergenic
TAGGTTCCTGTATAGAAAAT+TGG 0.294506 1_4:+46498795 Msa0165740:CDS
TGTGAGAAATGATTCTGTTT+TGG 0.294906 1_4:-46498519 None:intergenic
TAGAAAATTGGACTACAAAA+TGG 0.300863 1_4:+46498807 Msa0165740:CDS
TGATGCTTGTCTTACTCCTT+TGG 0.322877 1_4:-46500022 None:intergenic
ACATCATACCTGCCTTTAAC+AGG 0.328556 1_4:-46498970 None:intergenic
GCGCTTCTTGTGACAATCTA+TGG 0.350078 1_4:-46499890 None:intergenic
CAAGAAGTGAATATTTGAAT+AGG 0.369741 1_4:+46498288 Msa0165740:CDS
ATCCAAATAGACAATCTTCC+AGG 0.378879 1_4:+46498356 Msa0165740:CDS
TTTAAGGAATCAGCAACTAT+AGG 0.385762 1_4:-46497731 None:intergenic
TGAATAGGCTTGTCTTCAAA+AGG 0.388500 1_4:+46498303 Msa0165740:CDS
ATGTGTTTATGTGTCACATT+AGG 0.404324 1_4:-46498857 None:intergenic
ACATGTTCACTTGTTCTAGA+AGG 0.404709 1_4:-46499968 None:intergenic
AGCTATTGACATGTACCTTA+AGG 0.420374 1_4:+46499315 Msa0165740:CDS
TTGTAACTCCTTGGCCCAAT+GGG 0.422828 1_4:-46498603 None:intergenic
TTGTGAATCAATTTCCCATT+GGG 0.436436 1_4:+46498589 Msa0165740:CDS
TTACTTGATTCAGGTTTGCC+AGG 0.439410 1_4:+46497874 Msa0165740:intron
AGAAAATGTGTGTAATGTAT+TGG 0.445599 1_4:+46499863 Msa0165740:CDS
AAATCCTGTTGATAAGTTCA+TGG 0.445770 1_4:-46499943 None:intergenic
TTCCAGCTTTCACTTGCATA+AGG 0.448370 1_4:+46497935 Msa0165740:CDS
GTGTTGTGTTCAAGATATCT+TGG 0.448558 1_4:+46499054 Msa0165740:CDS
AAATCAAAGTTGATGCTTGT+TGG 0.453295 1_4:+46498684 Msa0165740:CDS
CACCTTATGCAAGTGAAAGC+TGG 0.456130 1_4:-46497937 None:intergenic
GAATAGGCTTGTCTTCAAAA+GGG 0.459367 1_4:+46498304 Msa0165740:CDS
GATATCTTGGTTAAGAATCA+AGG 0.460464 1_4:+46499067 Msa0165740:CDS
TTGCCCATGAACTTATCAAC+AGG 0.463591 1_4:+46499939 Msa0165740:CDS
AAAGGAGTAAGACAAGCATC+AGG 0.466683 1_4:+46500024 Msa0165740:CDS
GCTGCAAAAGTTCACAATGT+TGG 0.475366 1_4:+46499169 Msa0165740:CDS
ACAATATAATGAAGCTATGT+TGG 0.478289 1_4:-46498444 None:intergenic
TTTCACTAATTGTAACTCCT+TGG 0.480054 1_4:-46498612 None:intergenic
TTGTCAAATTCTGTTACGGT+TGG 0.488790 1_4:+46498402 Msa0165740:CDS
ATCAATTTCCCATTGGGCCA+AGG 0.494419 1_4:+46498595 Msa0165740:CDS
AGTTCACAATGTTGGAAGGT+TGG 0.507286 1_4:+46499177 Msa0165740:CDS
TTACTTCACCTGTTAAAGGC+AGG 0.511487 1_4:+46498962 Msa0165740:CDS
GTTGCTCAGAAGCAATTGCT+TGG 0.515310 1_4:+46498636 Msa0165740:CDS
TTAGTTGTCAAATTCTGTTA+CGG 0.519205 1_4:+46498398 Msa0165740:CDS
ATCATTAGCAGAACTTGCCC+TGG 0.519372 1_4:-46497892 None:intergenic
TAGTTGCTGATTCCTTAAAA+CGG 0.524321 1_4:+46497735 Msa0165740:CDS
GTTGTGAATCAATTTCCCAT+TGG 0.525573 1_4:+46498588 Msa0165740:CDS
ATTGTAACTCCTTGGCCCAA+TGG 0.529828 1_4:-46498604 None:intergenic
AATGATTTAATCATACAAGT+TGG 0.533319 1_4:+46497909 Msa0165740:CDS
AATCCTGTTGATAAGTTCAT+GGG 0.538863 1_4:-46499942 None:intergenic
GGAATGAAATCGGAGTTTGT+AGG 0.540139 1_4:+46498776 Msa0165740:CDS
GTTATCTATCACAAGTTGCA+AGG 0.555039 1_4:+46499206 Msa0165740:CDS
GCAACTATAGGAACATTAGC+TGG 0.557389 1_4:-46497719 None:intergenic
CAAAAGTTCACAATGTTGGA+AGG 0.563172 1_4:+46499173 Msa0165740:CDS
TAGAACAAGTGAACATGTCA+TGG 0.564910 1_4:+46499973 Msa0165740:CDS
GATGATGAAGTCACAACTGT+TGG 0.567585 1_4:+46500112 Msa0165740:CDS
TCACAATGTTGGAAGGTTGG+TGG 0.570684 1_4:+46499180 Msa0165740:CDS
GTTACGCTTTCCTCCACTCA+AGG 0.570870 1_4:-46497681 None:intergenic
TCAAAGTTGATGCTTGTTGG+TGG 0.571613 1_4:+46498687 Msa0165740:CDS
AATAGGCTTGTCTTCAAAAG+GGG 0.571861 1_4:+46498305 Msa0165740:CDS
TACTTGATTCAGGTTTGCCA+GGG 0.586307 1_4:+46497875 Msa0165740:intron
ATATTCACTTCTTGAGACCA+TGG 0.588159 1_4:-46498279 None:intergenic
TGGTTCTCTAAAGTCACACT+TGG 0.598171 1_4:+46498827 Msa0165740:CDS
TCATGGACAATGGCAAGTAG+TGG 0.603006 1_4:+46499990 Msa0165740:CDS
ATGAGTGAAGATATTGAACA+AGG 0.613707 1_4:+46498485 Msa0165740:CDS
GACTGTGAAAATGCCTTGAG+TGG 0.613781 1_4:+46497668 Msa0165740:CDS
AGTGAACATGTCATGGACAA+TGG 0.618994 1_4:+46499980 Msa0165740:CDS
TTATCTATCACAAGTTGCAA+GGG 0.626529 1_4:+46499207 Msa0165740:CDS
ATGTGTGCTAGAGAGAAGAG+TGG 0.638803 1_4:+46499015 Msa0165740:CDS
GTAGTGGATCAAATTACCAA+AGG 0.640603 1_4:+46500006 Msa0165740:CDS
TAATGTGACACATAAACACA+TGG 0.648727 1_4:+46498859 Msa0165740:CDS
AAGGAGTAAGACAAGCATCA+GGG 0.655380 1_4:+46500025 Msa0165740:CDS
AATGTTGGAAGGTTGGTGGA+TGG 0.656204 1_4:+46499184 Msa0165740:CDS
TGTGAAAATGCCTTGAGTGG+AGG 0.661907 1_4:+46497671 Msa0165740:CDS
CAAATAGACAATCTTCCAGG+TGG 0.702112 1_4:+46498359 Msa0165740:CDS
TAGCTGGTAGAACAACACAT+CGG 0.769094 1_4:-46497703 None:intergenic
AAGGGATCAGAAGCTCACAG+TGG 0.808993 1_4:+46499225 Msa0165740:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATATATATCTTATAATAAA+AGG - chr1_4:46497965-46497984 None:intergenic 10.0%
!!! TTTTAGTTATATCTACTTAT+TGG + chr1_4:46498076-46498095 Msa0165740:intron 15.0%
!! ATAATATTAGTATAACCTTA+AGG - chr1_4:46499333-46499352 None:intergenic 15.0%
!! TTAAAACTTCAATAAAATCA+CGG - chr1_4:46499574-46499593 None:intergenic 15.0%
!! AATGATTTAATCATACAAGT+TGG + chr1_4:46497909-46497928 Msa0165740:CDS 20.0%
!! ATTAAGTTATATATCACCAT+TGG + chr1_4:46498046-46498065 Msa0165740:intron 20.0%
!! GTAGATATAACTAAAAGTAA+TGG - chr1_4:46498072-46498091 None:intergenic 20.0%
!! TGTTATATAACTAAAAGCAA+TGG - chr1_4:46498102-46498121 None:intergenic 20.0%
!!! TATAGCTTTTGTAAAAATCA+TGG + chr1_4:46499596-46499615 Msa0165740:intron 20.0%
!!! TAAATTTAACTCAATTTTGC+AGG + chr1_4:46499812-46499831 Msa0165740:intron 20.0%
!!! GTTTTGATTTTTCCGTTTTA+AGG - chr1_4:46497750-46497769 None:intergenic 25.0%
! TAAAACGGAAAAATCAAAAC+TGG + chr1_4:46497750-46497769 Msa0165740:CDS 25.0%
! GTTTCAATAATGACATGTTT+GGG - chr1_4:46497792-46497811 None:intergenic 25.0%
! TGTTTCAATAATGACATGTT+TGG - chr1_4:46497793-46497812 None:intergenic 25.0%
! CAAGAAGTGAATATTTGAAT+AGG + chr1_4:46498288-46498307 Msa0165740:CDS 25.0%
! TTAGTTGTCAAATTCTGTTA+CGG + chr1_4:46498398-46498417 Msa0165740:CDS 25.0%
! ACAATATAATGAAGCTATGT+TGG - chr1_4:46498447-46498466 None:intergenic 25.0%
! CATTTCTAATGTTATCTTCA+TGG + chr1_4:46498540-46498559 Msa0165740:CDS 25.0%
!!! TCAACTTTGATTTCAAGTTT+TGG - chr1_4:46498677-46498696 None:intergenic 25.0%
! ATTCAATCTATCAAAAAACG+TGG + chr1_4:46498755-46498774 Msa0165740:CDS 25.0%
! TAGAAAATTGGACTACAAAA+TGG + chr1_4:46498807-46498826 Msa0165740:CDS 25.0%
!!! AAGCATTTTAATCAAACACT+CGG - chr1_4:46498909-46498928 None:intergenic 25.0%
!!! TTTTTTACTTCACCTGTTAA+AGG + chr1_4:46498958-46498977 Msa0165740:CDS 25.0%
!!! TACTTCAAGTTTTATTTGTG+TGG + chr1_4:46499699-46499718 Msa0165740:intron 25.0%
! CATATGTCTCTATGATTATA+CGG + chr1_4:46499770-46499789 Msa0165740:intron 25.0%
! AGAAAATGTGTGTAATGTAT+TGG + chr1_4:46499863-46499882 Msa0165740:CDS 25.0%
!! ATGAACAAGTTTGAGTTTTC+TGG + chr1_4:46497617-46497636 Msa0165740:CDS 30.0%
! TTCACAGTCAAGAATTTTCA+TGG - chr1_4:46497657-46497676 None:intergenic 30.0%
TTTAAGGAATCAGCAACTAT+AGG - chr1_4:46497734-46497753 None:intergenic 30.0%
!! TAGTTGCTGATTCCTTAAAA+CGG + chr1_4:46497735-46497754 Msa0165740:CDS 30.0%
ATGTCTCATTTACTTGATTC+AGG + chr1_4:46497865-46497884 Msa0165740:intron 30.0%
TAACTAAAAGTAATGGCCAA+TGG - chr1_4:46498065-46498084 None:intergenic 30.0%
GCTATGATATGCTTGAAATT+AGG - chr1_4:46498197-46498216 None:intergenic 30.0%
ATGAGTGAAGATATTGAACA+AGG + chr1_4:46498485-46498504 Msa0165740:CDS 30.0%
! TGTGAGAAATGATTCTGTTT+TGG - chr1_4:46498522-46498541 None:intergenic 30.0%
TTGTGAATCAATTTCCCATT+GGG + chr1_4:46498589-46498608 Msa0165740:CDS 30.0%
TTTCACTAATTGTAACTCCT+TGG - chr1_4:46498615-46498634 None:intergenic 30.0%
!! AAATCAAAGTTGATGCTTGT+TGG + chr1_4:46498684-46498703 Msa0165740:CDS 30.0%
CAAAAAACGTGGAATGAAAT+CGG + chr1_4:46498766-46498785 Msa0165740:CDS 30.0%
TAGGTTCCTGTATAGAAAAT+TGG + chr1_4:46498795-46498814 Msa0165740:CDS 30.0%
! TGTAGTCCAATTTTCTATAC+AGG - chr1_4:46498804-46498823 None:intergenic 30.0%
ATGTGTTTATGTGTCACATT+AGG - chr1_4:46498860-46498879 None:intergenic 30.0%
TAATGTGACACATAAACACA+TGG + chr1_4:46498859-46498878 Msa0165740:CDS 30.0%
GATATCTTGGTTAAGAATCA+AGG + chr1_4:46499067-46499086 Msa0165740:CDS 30.0%
GTGTTACAATCTTATGTTCT+TGG + chr1_4:46499130-46499149 Msa0165740:CDS 30.0%
TTATCTATCACAAGTTGCAA+GGG + chr1_4:46499207-46499226 Msa0165740:CDS 30.0%
! TCACAATCTCTACAATTTTG+TGG - chr1_4:46499280-46499299 None:intergenic 30.0%
! GTTTGGTTCAACAATTAGTT+TGG + chr1_4:46499532-46499551 Msa0165740:intron 30.0%
! AGCTTTTGTAAAAATCATGG+TGG + chr1_4:46499599-46499618 Msa0165740:intron 30.0%
AGAGACATATGCAAACAATA+TGG - chr1_4:46499761-46499780 None:intergenic 30.0%
CTATCTTCTTCTGTCAAATA+TGG - chr1_4:46499843-46499862 None:intergenic 30.0%
AGCATGTGATGAAAAATGAT+AGG + chr1_4:46499916-46499935 Msa0165740:CDS 30.0%
! AATCCTGTTGATAAGTTCAT+GGG - chr1_4:46499945-46499964 None:intergenic 30.0%
! AAATCCTGTTGATAAGTTCA+TGG - chr1_4:46499946-46499965 None:intergenic 30.0%
! TGCATAACTTCAAATGCTTT+TGG - chr1_4:46500163-46500182 None:intergenic 30.0%
TGCTTGAAATTAGGAACATG+AGG - chr1_4:46498188-46498207 None:intergenic 35.0%
ATATTCACTTCTTGAGACCA+TGG - chr1_4:46498282-46498301 None:intergenic 35.0%
TGAATAGGCTTGTCTTCAAA+AGG + chr1_4:46498303-46498322 Msa0165740:CDS 35.0%
GAATAGGCTTGTCTTCAAAA+GGG + chr1_4:46498304-46498323 Msa0165740:CDS 35.0%
AATAGGCTTGTCTTCAAAAG+GGG + chr1_4:46498305-46498324 Msa0165740:CDS 35.0%
ATCCAAATAGACAATCTTCC+AGG + chr1_4:46498356-46498375 Msa0165740:CDS 35.0%
! TCAAATGTTTTTCTACCACC+TGG - chr1_4:46498377-46498396 None:intergenic 35.0%
TTGTCAAATTCTGTTACGGT+TGG + chr1_4:46498402-46498421 Msa0165740:CDS 35.0%
GTTGTGAATCAATTTCCCAT+TGG + chr1_4:46498588-46498607 Msa0165740:CDS 35.0%
! GAGACAGAATTTTCTTCAGT+TGG - chr1_4:46498932-46498951 None:intergenic 35.0%
GTGTTGTGTTCAAGATATCT+TGG + chr1_4:46499054-46499073 Msa0165740:CDS 35.0%
CAAAAGTTCACAATGTTGGA+AGG + chr1_4:46499173-46499192 Msa0165740:CDS 35.0%
GTTATCTATCACAAGTTGCA+AGG + chr1_4:46499206-46499225 Msa0165740:CDS 35.0%
AGCTATTGACATGTACCTTA+AGG + chr1_4:46499315-46499334 Msa0165740:CDS 35.0%
AATCACAGTCATATTCGAGT+TGG + chr1_4:46499375-46499394 Msa0165740:intron 35.0%
! ATCACAGTCATATTCGAGTT+GGG + chr1_4:46499376-46499395 Msa0165740:intron 35.0%
TGCATACAATCAGTCAAATG+TGG + chr1_4:46499410-46499429 Msa0165740:intron 35.0%
CCAAACATGCACTTAAAAAC+AGG - chr1_4:46499518-46499537 None:intergenic 35.0%
!!! CCTGTTTTTAAGTGCATGTT+TGG + chr1_4:46499515-46499534 Msa0165740:intron 35.0%
! ACATGTTCACTTGTTCTAGA+AGG - chr1_4:46499971-46499990 None:intergenic 35.0%
TAGAACAAGTGAACATGTCA+TGG + chr1_4:46499973-46499992 Msa0165740:CDS 35.0%
!! GTAGTGGATCAAATTACCAA+AGG + chr1_4:46500006-46500025 Msa0165740:CDS 35.0%
TAGCTGGTAGAACAACACAT+CGG - chr1_4:46497706-46497725 None:intergenic 40.0%
GCAACTATAGGAACATTAGC+TGG - chr1_4:46497722-46497741 None:intergenic 40.0%
! TTACTTGATTCAGGTTTGCC+AGG + chr1_4:46497874-46497893 Msa0165740:intron 40.0%
! TACTTGATTCAGGTTTGCCA+GGG + chr1_4:46497875-46497894 Msa0165740:intron 40.0%
TTCCAGCTTTCACTTGCATA+AGG + chr1_4:46497935-46497954 Msa0165740:CDS 40.0%
! TAGGAACATGAGGTGCTTAT+TGG - chr1_4:46498178-46498197 None:intergenic 40.0%
CACCTGGAAGATTGTCTATT+TGG - chr1_4:46498361-46498380 None:intergenic 40.0%
CAAATAGACAATCTTCCAGG+TGG + chr1_4:46498359-46498378 Msa0165740:CDS 40.0%
!! TCAAAGTTGATGCTTGTTGG+TGG + chr1_4:46498687-46498706 Msa0165740:CDS 40.0%
GGAATGAAATCGGAGTTTGT+AGG + chr1_4:46498776-46498795 Msa0165740:CDS 40.0%
!! TGGTTCTCTAAAGTCACACT+TGG + chr1_4:46498827-46498846 Msa0165740:CDS 40.0%
TTACTTCACCTGTTAAAGGC+AGG + chr1_4:46498962-46498981 Msa0165740:CDS 40.0%
ACATCATACCTGCCTTTAAC+AGG - chr1_4:46498973-46498992 None:intergenic 40.0%
!!! GAGAAGAGTGGTTTTGATGT+TGG + chr1_4:46499027-46499046 Msa0165740:CDS 40.0%
GCTGCAAAAGTTCACAATGT+TGG + chr1_4:46499169-46499188 Msa0165740:CDS 40.0%
AGTTCACAATGTTGGAAGGT+TGG + chr1_4:46499177-46499196 Msa0165740:CDS 40.0%
TTGCCCATGAACTTATCAAC+AGG + chr1_4:46499939-46499958 Msa0165740:CDS 40.0%
AGTGAACATGTCATGGACAA+TGG + chr1_4:46499980-46499999 Msa0165740:CDS 40.0%
TGATGCTTGTCTTACTCCTT+TGG - chr1_4:46500025-46500044 None:intergenic 40.0%
AAAGGAGTAAGACAAGCATC+AGG + chr1_4:46500024-46500043 Msa0165740:CDS 40.0%
AAGGAGTAAGACAAGCATCA+GGG + chr1_4:46500025-46500044 Msa0165740:CDS 40.0%
GATGATGAAGTCACAACTGT+TGG + chr1_4:46500112-46500131 Msa0165740:CDS 40.0%
GACTGTGAAAATGCCTTGAG+TGG + chr1_4:46497668-46497687 Msa0165740:CDS 45.0%
TGTGAAAATGCCTTGAGTGG+AGG + chr1_4:46497671-46497690 Msa0165740:CDS 45.0%
ATCATTAGCAGAACTTGCCC+TGG - chr1_4:46497895-46497914 None:intergenic 45.0%
CACCTTATGCAAGTGAAAGC+TGG - chr1_4:46497940-46497959 None:intergenic 45.0%
CAGCTTTCACTTGCATAAGG+TGG + chr1_4:46497938-46497957 Msa0165740:intron 45.0%
!! GTTGATTTTCCAGCTTGCCA+TGG + chr1_4:46498262-46498281 Msa0165740:intron 45.0%
ATCAATTTCCCATTGGGCCA+AGG + chr1_4:46498595-46498614 Msa0165740:CDS 45.0%
TTGTAACTCCTTGGCCCAAT+GGG - chr1_4:46498606-46498625 None:intergenic 45.0%
ATTGTAACTCCTTGGCCCAA+TGG - chr1_4:46498607-46498626 None:intergenic 45.0%
GTTGCTCAGAAGCAATTGCT+TGG + chr1_4:46498636-46498655 Msa0165740:CDS 45.0%
ATGTGTGCTAGAGAGAAGAG+TGG + chr1_4:46499015-46499034 Msa0165740:CDS 45.0%
TCACAATGTTGGAAGGTTGG+TGG + chr1_4:46499180-46499199 Msa0165740:CDS 45.0%
!! AATGTTGGAAGGTTGGTGGA+TGG + chr1_4:46499184-46499203 Msa0165740:CDS 45.0%
! CAGGTTTGCGACTTTGACTT+GGG - chr1_4:46499499-46499518 None:intergenic 45.0%
ACAGGTTTGCGACTTTGACT+TGG - chr1_4:46499500-46499519 None:intergenic 45.0%
GCGCTTCTTGTGACAATCTA+TGG - chr1_4:46499893-46499912 None:intergenic 45.0%
TCATGGACAATGGCAAGTAG+TGG + chr1_4:46499990-46500009 Msa0165740:CDS 45.0%
GTTACGCTTTCCTCCACTCA+AGG - chr1_4:46497684-46497703 None:intergenic 50.0%
TTCTTGAGACCATGGCAAGC+TGG - chr1_4:46498274-46498293 None:intergenic 50.0%
AAGGGATCAGAAGCTCACAG+TGG + chr1_4:46499225-46499244 Msa0165740:CDS 50.0%
TGGCTGATCCACCTCAATCG+TGG + chr1_4:46499430-46499449 Msa0165740:intron 55.0%
ATCGTGGTCGTGCAGCAGTT+TGG + chr1_4:46499446-46499465 Msa0165740:intron 55.0%
CTGCACGACCACGATTGAGG+TGG - chr1_4:46499441-46499460 None:intergenic 60.0%
CTGCTGCACGACCACGATTG+AGG - chr1_4:46499444-46499463 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr1_4 gene 46497607 46500190 46497607 ID=Msa0165740;Name=Msa0165740
chr1_4 mRNA 46497607 46500190 46497607 ID=Msa0165740-mRNA-1;Parent=Msa0165740;Name=Msa0165740-mRNA-1;_AED=0.10;_eAED=0.10;_QI=10|1|0.5|1|1|1|4|0|547
chr1_4 exon 46497607 46497771 46497607 ID=Msa0165740-mRNA-1:exon:13365;Parent=Msa0165740-mRNA-1
chr1_4 exon 46497887 46497956 46497887 ID=Msa0165740-mRNA-1:exon:13366;Parent=Msa0165740-mRNA-1
chr1_4 exon 46498275 46499336 46498275 ID=Msa0165740-mRNA-1:exon:13367;Parent=Msa0165740-mRNA-1
chr1_4 exon 46499834 46500190 46499834 ID=Msa0165740-mRNA-1:exon:13368;Parent=Msa0165740-mRNA-1
chr1_4 five_prime_UTR 46497607 46497616 46497607 ID=Msa0165740-mRNA-1:five_prime_utr;Parent=Msa0165740-mRNA-1
chr1_4 CDS 46497617 46497771 46497617 ID=Msa0165740-mRNA-1:cds;Parent=Msa0165740-mRNA-1
chr1_4 CDS 46497887 46497956 46497887 ID=Msa0165740-mRNA-1:cds;Parent=Msa0165740-mRNA-1
chr1_4 CDS 46498275 46499336 46498275 ID=Msa0165740-mRNA-1:cds;Parent=Msa0165740-mRNA-1
chr1_4 CDS 46499834 46500190 46499834 ID=Msa0165740-mRNA-1:cds;Parent=Msa0165740-mRNA-1
Gene Sequence

>Msa0165740

ATGAACAAGTTTGAGTTTTCTGGTGTTGATAAAGCGACCATGAAAATTCTTGACTGTGAAAATGCCTTGAGTGGAGGAAAGCGTAACCGATGTGTTGTTCTACCAGCTAATGTTCCTATAGTTGCTGATTCCTTAAAACGGAAAAATCAAAACTGGTTTGCCAGGGCAAGTTCTGCTAATGATTTAATCATACAAGTTGGTGATTCCAGCTTTCACTTGCATAAGCTTGCCATGGTCTCAAGAAGTGAATATTTGAATAGGCTTGTCTTCAAAAGGGGAAGTAACATAGAAGACAATAATCATACTATCCAAATAGACAATCTTCCAGGTGGTAGAAAAACATTTGAGTTAGTTGTCAAATTCTGTTACGGTTGGAAATTTGATATGACATCATCCAACATAGCTTCATTATATTGTGCTGCAAATTTCTTAGAAATGAGTGAAGATATTGAACAAGGAAATCTAATTTCCAAAACAGAATCATTTCTCACATTTCTAATGTTATCTTCATGGAAAGACACATTTCGTATACTCAAAAGTTGTGAATCAATTTCCCATTGGGCCAAGGAGTTACAATTAGTGAAACGTTGCTCAGAAGCAATTGCTTGGAAAGCATGTGCAATTCCAAAACTTGAAATCAAAGTTGATGCTTGTTGGTGGTTTGAAGATGTATCACTTCTACGTATAGATCATTTTATTGAAGTGATTCAATCTATCAAAAAACGTGGAATGAAATCGGAGTTTGTAGGTTCCTGTATAGAAAATTGGACTACAAAATGGTTCTCTAAAGTCACACTTGGACTTGATCCTAATGTGACACATAAACACATGGCAATTCAGTTACATAAAGTTTCAACCGAGTGTTTGATTAAAATGCTTCCAACTGAAGAAAATTCTGTCTCTTGCAATTTTTTACTTCACCTGTTAAAGGCAGGTATGATGTTGAAAATCAATCATGAGTTGTTATGTGTGCTAGAGAGAAGAGTGGTTTTGATGTTGGAGCAGTGTTGTGTTCAAGATATCTTGGTTAAGAATCAAGGAGATAAAGATTCTTTGTATGATGTTGATGTTGTTGTTAGAGTGTTACAATCTTATGTTCTTGGCATTTCAAGTAATTCTGCTGCAAAAGTTCACAATGTTGGAAGGTTGGTGGATGGTTATCTATCACAAGTTGCAAGGGATCAGAAGCTCACAGTGGAAAGTTTCAAATTGCTTGTTGAAGTATTGCCACAAAATTGTAGAGATTGTGATGACAATCTCTATAGAGCTATTGACATGTACCTTAAGGCACATCCATATTTGACAGAAGAAGATAGAGAAAATGTGTGTAATGTATTGGAATACCATAGATTGTCACAAGAAGCGCGTCAGCATGTGATGAAAAATGATAGGTTGCCCATGAACTTATCAACAGGATTTGTCCTTCTAGAACAAGTGAACATGTCATGGACAATGGCAAGTAGTGGATCAAATTACCAAAGGAGTAAGACAAGCATCAGGGTAAGCAAAGATTTTGAAAACAGACAGATGAATCAAGAGATAAAAGTAATGAGAAAAGATGTTGAGATGATGAAGTCACAACTGTTGGAACTAAATACTTGCAAAATGAAGCTCCAAAAGCATTTGAAGTTATGCACTCGTTGA
Protein sequence

>Msa0165740

MNKFEFSGVDKATMKILDCENALSGGKRNRCVVLPANVPIVADSLKRKNQNWFARASSANDLIIQVGDSSFHLHKLAMVSRSEYLNRLVFKRGSNIEDNNHTIQIDNLPGGRKTFELVVKFCYGWKFDMTSSNIASLYCAANFLEMSEDIEQGNLISKTESFLTFLMLSSWKDTFRILKSCESISHWAKELQLVKRCSEAIAWKACAIPKLEIKVDACWWFEDVSLLRIDHFIEVIQSIKKRGMKSEFVGSCIENWTTKWFSKVTLGLDPNVTHKHMAIQLHKVSTECLIKMLPTEENSVSCNFLLHLLKAGMMLKINHELLCVLERRVVLMLEQCCVQDILVKNQGDKDSLYDVDVVVRVLQSYVLGISSNSAAKVHNVGRLVDGYLSQVARDQKLTVESFKLLVEVLPQNCRDCDDNLYRAIDMYLKAHPYLTEEDRENVCNVLEYHRLSQEARQHVMKNDRLPMNLSTGFVLLEQVNMSWTMASSGSNYQRSKTSIRVSKDFENRQMNQEIKVMRKDVEMMKSQLLELNTCKMKLQKHLKLCTR*