AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1358170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1358170 MtrunA17_Chr5g0397601 96.624 1007 8 2 1 981 1 1007 0.0 1998
Msa1358170 MtrunA17_Chr8g0349231 62.106 1016 337 14 1 981 1 1003 0.0 1276
Msa1358170 MtrunA17_Chr7g0258331 66.496 779 223 7 232 981 6 775 0.0 1097
Msa1358170 MtrunA17_Chr1g0174761 66.152 777 228 5 232 981 6 774 0.0 1078
Msa1358170 MtrunA17_Chr4g0033151 64.942 773 235 6 234 981 9 770 0.0 1072
Msa1358170 MtrunA17_Chr1g0174581 59.466 787 277 7 234 981 14 797 0.0 993
Msa1358170 MtrunA17_Chr3g0087501 59.924 791 271 10 234 981 35 822 0.0 993
Msa1358170 MtrunA17_Chr4g0010751 61.559 757 254 7 258 981 442 1194 0.0 989
Msa1358170 MtrunA17_Chr4g0010751 35.359 181 116 1 4 183 1 181 1.80e-25 114
Msa1358170 MtrunA17_Chr4g0014711 59.719 782 274 10 231 981 273 1044 0.0 959
Msa1358170 MtrunA17_Chr7g0247271 61.760 693 222 8 325 981 281 966 0.0 875
Msa1358170 MtrunA17_Chr7g0247271 31.864 590 326 19 1 526 78 655 7.31e-62 228
Msa1358170 MtrunA17_Chr6g0461151 41.579 1051 510 21 5 981 6 1026 0.0 783
Msa1358170 MtrunA17_Chr4g0028371 49.303 789 344 11 231 981 13 783 0.0 769
Msa1358170 MtrunA17_Chr4g0035961 49.540 761 327 11 234 981 16 732 0.0 759
Msa1358170 MtrunA17_Chr4g0060191 49.409 761 317 13 264 981 303 1038 0.0 736
Msa1358170 MtrunA17_Chr4g0060191 49.242 132 63 1 5 132 18 149 1.71e-30 130
Msa1358170 MtrunA17_Chr6g0460791 47.313 763 330 15 264 981 274 1009 0.0 708
Msa1358170 MtrunA17_Chr6g0460791 44.961 129 70 1 5 132 11 139 2.88e-29 126
Msa1358170 MtrunA17_Chr1g0151051 40.571 806 417 14 220 981 11 798 0.0 586
Msa1358170 MtrunA17_Chr5g0447321 37.705 793 423 17 229 981 2 763 9.32e-176 530
Msa1358170 MtrunA17_Chr4g0010641 60.000 130 50 1 851 980 1 128 4.26e-48 167
Msa1358170 MtrunA17_Chr6g0461171 40.310 129 75 1 5 131 6 134 3.91e-26 107
Msa1358170 MtrunA17_Chr4g0010701 44.231 104 36 2 854 957 16 97 2.47e-20 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1358170 AT4G11610.2 73.262 1021 217 8 1 981 3 1007 0.0 1561
Msa1358170 AT4G11610.1 73.066 1021 223 8 1 981 3 1011 0.0 1558
Msa1358170 AT4G11610.3 74.505 859 177 6 163 981 18 874 0.0 1342
Msa1358170 AT4G00700.1 54.990 1022 403 11 1 981 1 1006 0.0 1135
Msa1358170 AT4G00700.2 54.990 1022 403 11 1 981 1 1006 0.0 1135
Msa1358170 AT3G57880.4 67.439 777 217 7 232 981 6 773 0.0 1113
Msa1358170 AT3G57880.2 67.439 777 217 7 232 981 6 773 0.0 1113
Msa1358170 AT3G57880.3 67.439 777 217 7 232 981 6 773 0.0 1113
Msa1358170 AT3G57880.1 67.439 777 217 7 232 981 6 773 0.0 1113
Msa1358170 AT5G12970.1 67.010 776 219 5 231 981 6 769 0.0 1089
Msa1358170 AT1G51570.1 66.110 779 227 6 232 981 6 776 0.0 1089
Msa1358170 AT1G22610.1 52.901 1034 423 15 5 981 3 1029 0.0 1072
Msa1358170 AT3G61300.1 52.182 1031 383 23 1 981 2 972 0.0 1048
Msa1358170 AT5G48060.1 50.895 1061 407 17 5 981 6 1036 0.0 1046
Msa1358170 AT5G06850.1 59.796 786 275 7 234 981 12 794 0.0 1013
Msa1358170 AT1G04150.1 54.950 808 299 15 216 981 228 1012 0.0 900
Msa1358170 AT4G20080.1 53.659 779 310 12 235 981 15 774 0.0 836
Msa1358170 AT1G74720.1 42.438 1091 493 24 2 981 15 1081 0.0 834
Msa1358170 AT5G17980.1 40.622 1061 529 21 5 981 6 1049 0.0 768
Msa1358170 AT3G03680.1 47.809 776 315 20 264 981 274 1017 0.0 719
Msa1358170 AT3G03680.1 41.406 128 75 0 5 132 8 135 1.28e-26 118
Msa1358170 AT3G61720.1 38.042 807 423 19 234 981 7 795 2.33e-159 489
Msa1358170 AT5G03435.1 39.896 767 401 25 234 974 7 739 4.84e-151 466
Msa1358170 AT5G03435.2 42.487 579 298 16 234 806 7 556 3.80e-128 399
Msa1358170 AT5G44760.1 47.044 406 164 10 234 634 5 364 3.80e-108 344
Msa1358170 AT5G44760.1 36.607 112 47 1 772 883 364 451 1.92e-17 87.0
Msa1358170 AT1G03370.2 36.885 122 73 2 273 392 3 122 1.54e-14 79.0
Msa1358170 AT1G03370.1 36.885 122 73 2 273 392 3 122 1.79e-14 78.6

Find 221 sgRNAs with CRISPR-Local

Find 267 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCAACTGGTGTTAAGAAAA+TGG 0.190357 tig0016220:+353468 Msa1358170:CDS
CACAACAAAGACCTGAAAAT+TGG 0.202269 tig0016220:+353370 Msa1358170:CDS
AGAAGAGCCTTCTCCATCTT+TGG 0.233433 tig0016220:-351391 None:intergenic
CTATCTGGTGGAACTCTTAA+AGG 0.240838 tig0016220:-352416 None:intergenic
ATTATGATCAACTTAAAGTT+AGG 0.248856 tig0016220:+351336 None:intergenic
GGCTCGTGAGCTTCCTTCTA+TGG 0.253047 tig0016220:+352175 Msa1358170:CDS
ACTAACTCAAGTTCATTTCT+TGG 0.262659 tig0016220:+351579 Msa1358170:CDS
GTGTATTGCTCGTTGTATTT+TGG 0.268384 tig0016220:-353271 None:intergenic
CTTCCTTCTATGGATCTTAC+TGG 0.278848 tig0016220:+352185 Msa1358170:CDS
TAATGGTTGTTCGGAACTTT+TGG 0.283980 tig0016220:-351443 None:intergenic
TTTCTGTTTGTTAGCTGCTT+TGG 0.314831 tig0016220:+354330 Msa1358170:CDS
TGAACTTGAGTTAGTAGCTT+TGG 0.320839 tig0016220:-351571 None:intergenic
GTGTGTTTGCAGTTGGAAAA+TGG 0.332879 tig0016220:+353776 Msa1358170:CDS
CTGATTCTTATCATAGTGTT+TGG 0.343905 tig0016220:-352258 None:intergenic
AAAAGGATCTTAATCCTGTT+TGG 0.345946 tig0016220:+351469 Msa1358170:CDS
TGCCGATGATGGTTTGTGTT+AGG 0.347471 tig0016220:-351882 None:intergenic
TGAAGATCATGTAATACTTT+CGG 0.351594 tig0016220:+352823 Msa1358170:CDS
TGAACAAGGACTGTTGTAAT+AGG 0.354570 tig0016220:-353826 None:intergenic
CAGATGTTGACTCACATCTA+TGG 0.373205 tig0016220:+353701 Msa1358170:CDS
TTGCTTGTTCTTCACCCAAC+TGG 0.374868 tig0016220:+353454 Msa1358170:CDS
TCGGGTGCCAAAGGACTATC+TGG 0.383261 tig0016220:-352431 None:intergenic
ACAAACGGTTGTAGGTGATT+TGG 0.383411 tig0016220:+354237 Msa1358170:CDS
GAAGAGCCTTCTCCATCTTT+GGG 0.384950 tig0016220:-351390 None:intergenic
GGTTGAAGATCGAGTTGGTC+TGG 0.386727 tig0016220:+352844 Msa1358170:CDS
TTCCCAAATCAGTGGTGAAA+AGG 0.392570 tig0016220:+353345 Msa1358170:CDS
CAAATGGTGGCTGGTTTAGC+AGG 0.392665 tig0016220:+354373 Msa1358170:CDS
AAGAAAGGGGAGAAAGTTAA+AGG 0.395492 tig0016220:+352473 Msa1358170:CDS
CTCGGTGGAGGAAGGGTAGT+TGG 0.396120 tig0016220:+352068 Msa1358170:CDS
GTCGTCAAGTCGATACCATT+CGG 0.399441 tig0016220:-352450 None:intergenic
CCATTTGTTGAGGTGAGAAT+TGG 0.401775 tig0016220:+352218 Msa1358170:CDS
ATAGAATCATTCATTCTAGA+TGG 0.401919 tig0016220:+352930 Msa1358170:CDS
TAGCAAAATACGGTCACAAA+TGG 0.411100 tig0016220:+353215 Msa1358170:CDS
TGTAGGTGATTTGGCTAGCC+AGG 0.412061 tig0016220:+354246 Msa1358170:CDS
CCAATTCTCACCTCAACAAA+TGG 0.417160 tig0016220:-352218 None:intergenic
AAAGATCTTCATTCCATTGT+GGG 0.420444 tig0016220:-352778 None:intergenic
TACCGCATTCTTACAAGGTC+AGG 0.427894 tig0016220:-354184 None:intergenic
AAGGACTCAACAGAAGGATT+TGG 0.428137 tig0016220:-351756 None:intergenic
GTGAGTTGATGCTAGCTGTT+TGG 0.429438 tig0016220:+352495 Msa1358170:CDS
TTATGATCAACTTAAAGTTA+GGG 0.431458 tig0016220:+351337 None:intergenic
AATTCCTCATCCATTTCATC+GGG 0.432095 tig0016220:-354136 None:intergenic
AACATACTATCAGTTCTTGC+AGG 0.433599 tig0016220:-354469 None:intergenic
CTGGGAAAAGATGAGATCAT+TGG 0.433880 tig0016220:+352863 Msa1358170:CDS
TGATCAACATCCACAGCAAC+AGG 0.435632 tig0016220:-352968 None:intergenic
TCAGAATCCGGAGTGGCATC+AGG 0.438503 tig0016220:+352277 Msa1358170:CDS
TCTTTAAGTGCAAAATCAAC+CGG 0.439305 tig0016220:-352032 None:intergenic
AAAGAAACAAGTCCGTTTCT+CGG 0.443107 tig0016220:+352050 Msa1358170:CDS
CTCAGTTACATCACTACAAC+CGG 0.443547 tig0016220:+352013 Msa1358170:CDS
CGCATATCGACTCTTGAAAC+AGG 0.446486 tig0016220:+353406 Msa1358170:CDS
ACTTCAGCATGATTATTTGT+TGG 0.446986 tig0016220:-351780 None:intergenic
TTCATGTGTGGAGGGTATCT+TGG 0.447757 tig0016220:-354082 None:intergenic
TTCTCTGGTGTGTTTGCAGT+TGG 0.450907 tig0016220:+353769 Msa1358170:CDS
GCCGATGATGGTTTGTGTTA+GGG 0.452215 tig0016220:-351881 None:intergenic
ATCGACTTGACGACAAGAAA+GGG 0.460087 tig0016220:+352459 Msa1358170:CDS
CCAACTGGTGTTAAGAAAAT+GGG 0.460536 tig0016220:+353469 Msa1358170:CDS
AATTCAGCTACGGCTATGTC+TGG 0.461073 tig0016220:+353018 Msa1358170:CDS
ACAACAAAGACCTGAAAATT+GGG 0.461807 tig0016220:+353371 Msa1358170:CDS
CCGAGTTCTAAAACACCAAT+TGG 0.462500 tig0016220:-353121 None:intergenic
CTCCCAGTAAGATCCATAGA+AGG 0.463798 tig0016220:-352188 None:intergenic
GCCCCACCGCAAAACAGTTA+TGG 0.466554 tig0016220:+353092 Msa1358170:CDS
CCGAACAACCATTAAAGAAA+AGG 0.467128 tig0016220:+351452 Msa1358170:CDS
TAGAAGCTCTCATTCCAAAC+AGG 0.467298 tig0016220:-351483 None:intergenic
GGATTCATGCACTGTTGAGC+TGG 0.468052 tig0016220:+354275 Msa1358170:CDS
TGCACCTTGACATATGCATC+CGG 0.472060 tig0016220:-352704 None:intergenic
GGAAGCCTGTGTCGAAATCT+CGG 0.473709 tig0016220:-354415 None:intergenic
CTCGGAGTGCTGAATGCAAT+AGG 0.474307 tig0016220:+353139 Msa1358170:CDS
TCGTCAAGTCGATACCATTC+GGG 0.474866 tig0016220:-352449 None:intergenic
GTGAGATAGTGTAGATTGGA+AGG 0.475750 tig0016220:-351522 None:intergenic
TCTATCATGCTCCGAGGTTG+TGG 0.475877 tig0016220:+352621 Msa1358170:CDS
GTTGAAGATCGAGTTGGTCT+GGG 0.477250 tig0016220:+352845 Msa1358170:CDS
TGGGAAAAGATGAGATCATT+GGG 0.477298 tig0016220:+352864 Msa1358170:CDS
TCCCAAATCAGTGGTGAAAA+GGG 0.479687 tig0016220:+353346 Msa1358170:CDS
AGCATGATTATTTGTTGGCA+AGG 0.480052 tig0016220:-351775 None:intergenic
TGACATATGCATCCGGGAAA+CGG 0.482033 tig0016220:-352697 None:intergenic
AAGAGTGAGATAGTGTAGAT+TGG 0.487071 tig0016220:-351526 None:intergenic
GCATATGTCAAGGTGCAGAT+AGG 0.488617 tig0016220:+352710 Msa1358170:CDS
AACAGAAGGATTTGGAATTG+AGG 0.489461 tig0016220:-351748 None:intergenic
CGTATCACTGTTGTAGTAGC+AGG 0.491771 tig0016220:-352590 None:intergenic
ACATATTGTGTAGCAAAATA+CGG 0.491890 tig0016220:+353205 Msa1358170:CDS
TGTAGTACACCCCGATGAAA+TGG 0.494237 tig0016220:+354126 Msa1358170:CDS
ACCCTTCCTCCACCGAGAAA+CGG 0.494679 tig0016220:-352062 None:intergenic
AGTTCCACCAGATAGTCCTT+TGG 0.496430 tig0016220:+352424 Msa1358170:CDS
GGTAGAATACAAACGGTTGT+AGG 0.496800 tig0016220:+354229 Msa1358170:CDS
GATCGTTTGAGGAGCGTGGC+TGG 0.499227 tig0016220:+354208 Msa1358170:CDS
CTTGGTAAAGTTAGACTCAC+AGG 0.500304 tig0016220:+351597 Msa1358170:CDS
TTTGTAGAACTTTACTTCGA+TGG 0.501129 tig0016220:+351420 Msa1358170:CDS
CTGCCACTATGTTTACTGCT+TGG 0.501177 tig0016220:-353624 None:intergenic
GAGAATGCAAGCATCTGTAC+TGG 0.502394 tig0016220:+352319 Msa1358170:CDS
GAAAATGGGCGAACTTCACT+TGG 0.503250 tig0016220:+353483 Msa1358170:CDS
AAATTCCTCATCCATTTCAT+CGG 0.503321 tig0016220:-354137 None:intergenic
CCTACGGGTTCAATGATGAA+TGG 0.504774 tig0016220:+351810 Msa1358170:CDS
AGTATTACATGATCTTCAAA+AGG 0.505539 tig0016220:-352818 None:intergenic
GTCCGTTTCTCGGTGGAGGA+AGG 0.506304 tig0016220:+352060 Msa1358170:CDS
TCATTCTAGATGGTTCAACT+TGG 0.507484 tig0016220:+352940 Msa1358170:CDS
AACATAGTGGCAGCGCGGCT+CGG 0.511333 tig0016220:+353634 Msa1358170:CDS
GCTGAAGTCCACGGACCTAC+GGG 0.511884 tig0016220:+351795 Msa1358170:CDS
TTTGCAGTTGGAAAATGGTT+AGG 0.514024 tig0016220:+353781 Msa1358170:CDS
AAAAGATCTTCATTCCATTG+TGG 0.517456 tig0016220:-352779 None:intergenic
CAGTTATGGAGGCCACCAAT+TGG 0.517888 tig0016220:+353106 Msa1358170:CDS
GAAAGAAGTTTGCCTTGCTA+CGG 0.518974 tig0016220:-353732 None:intergenic
TTTGTATGTCAGGGTCGTTA+AGG 0.519572 tig0016220:+352154 Msa1358170:CDS
AAACCAGCCACCATTTGAAA+AGG 0.520236 tig0016220:-354367 None:intergenic
ACAAGTCCGTTTCTCGGTGG+AGG 0.522181 tig0016220:+352056 Msa1358170:CDS
GTTGATGTTGATGCCGATGA+TGG 0.522344 tig0016220:-351893 None:intergenic
TCCCGAAAATGCATTACGTT+AGG 0.522411 tig0016220:+353566 Msa1358170:CDS
TTCCTTCTATGGATCTTACT+GGG 0.522971 tig0016220:+352186 Msa1358170:CDS
TGTACTAGCTTCATAGGTTG+TGG 0.523049 tig0016220:-351984 None:intergenic
TGCATGTGTACTAGCTTCAT+AGG 0.523340 tig0016220:-351990 None:intergenic
CACAATCTACTACCCAAAGA+TGG 0.523857 tig0016220:+351378 Msa1358170:CDS
TGCTGAAGTCCACGGACCTA+CGG 0.525914 tig0016220:+351794 Msa1358170:CDS
TTGAGTTAGTAGCTTTGGAA+TGG 0.526396 tig0016220:-351566 None:intergenic
TGTACTGGAAGTTGTGATCA+AGG 0.527389 tig0016220:+352334 Msa1358170:CDS
TGACAACTGCATCAGCTTGA+GGG 0.527617 tig0016220:-351632 None:intergenic
CTATGATAAGAATCAGAATC+CGG 0.528818 tig0016220:+352265 Msa1358170:CDS
AGCAAAATACGGTCACAAAT+GGG 0.529935 tig0016220:+353216 Msa1358170:CDS
TCTCGCGTAAGAGGTGAGCT+TGG 0.536637 tig0016220:+351687 Msa1358170:CDS
ATTTCATCAGCTTCATACTT+CGG 0.537653 tig0016220:-351948 None:intergenic
GAATGCGGTATGATCGTTTG+AGG 0.538021 tig0016220:+354197 Msa1358170:CDS
TAGTAGCTTTGGAATGGCAA+TGG 0.541351 tig0016220:-351560 None:intergenic
TTGATGCTAGCTGTTTGGAT+TGG 0.541583 tig0016220:+352500 Msa1358170:CDS
TACATGATCTTCAAAAGGCT+CGG 0.542481 tig0016220:-352813 None:intergenic
TATCGACTTGACGACAAGAA+AGG 0.544682 tig0016220:+352458 Msa1358170:CDS
CTTTCGGTTGAAGATCGAGT+TGG 0.546483 tig0016220:+352839 Msa1358170:CDS
ACCACTTCTTTACGAAGTGG+TGG 0.550403 tig0016220:-353667 None:intergenic
CTTGGAAAAGCCTGTTGCTG+TGG 0.552103 tig0016220:+352958 Msa1358170:CDS
AAGTTAGGGGTAGATGTGGT+TGG 0.552972 tig0016220:+351351 Msa1358170:CDS
AAGAAGTTGATCGGTGCAGA+CGG 0.553790 tig0016220:-354436 None:intergenic
GATCATACCGCATTCTTACA+AGG 0.554571 tig0016220:-354189 None:intergenic
GTAGGTGATTTGGCTAGCCA+GGG 0.554831 tig0016220:+354247 Msa1358170:CDS
TCCGTTTCTCGGTGGAGGAA+GGG 0.556359 tig0016220:+352061 Msa1358170:CDS
TGTGGGTTTAGAGTTCTAGC+AGG 0.559920 tig0016220:-352761 None:intergenic
ATGGAGCATGCGCCGTAGCA+AGG 0.566672 tig0016220:+353720 Msa1358170:CDS
AACTAGATCATATGTACTTG+CGG 0.568364 tig0016220:-352117 None:intergenic
TTCTTGTGTTCATGTGTGGA+GGG 0.569126 tig0016220:-354090 None:intergenic
ATTCTTGTGTTCATGTGTGG+AGG 0.569293 tig0016220:-354091 None:intergenic
ATGACAACTGCATCAGCTTG+AGG 0.569540 tig0016220:-351633 None:intergenic
CGCCTCTCCACAGCGTTCAG+CGG 0.570587 tig0016220:-352899 None:intergenic
ATTTGGCTAGCCAGGGTGAG+AGG 0.571757 tig0016220:+354254 Msa1358170:CDS
GGTGGAGGAAGGGTAGTTGG+TGG 0.571969 tig0016220:+352071 Msa1358170:CDS
CTTAAAGTTAGGGGTAGATG+TGG 0.572186 tig0016220:+351347 Msa1358170:CDS
GAGTCTTGATCCATTTGTTG+AGG 0.575055 tig0016220:+352208 Msa1358170:CDS
TCCCTAACACAAACCATCAT+CGG 0.575519 tig0016220:+351880 Msa1358170:CDS
GGCATCCGAGATTTCGACAC+AGG 0.577334 tig0016220:+354410 Msa1358170:CDS
CGTGGCTGGTAGAATACAAA+CGG 0.577843 tig0016220:+354222 Msa1358170:CDS
GTTGGCAAGGACTCAACAGA+AGG 0.579568 tig0016220:-351762 None:intergenic
CCTACTGTGAGGACTGTGGC+AGG 0.580485 tig0016220:-353310 None:intergenic
TAGCAGGTTTCTATTTCATG+AGG 0.582086 tig0016220:+354389 Msa1358170:CDS
CGATACCATTCGGGTGCCAA+AGG 0.582226 tig0016220:-352440 None:intergenic
CTACTACCCAAAGATGGAGA+AGG 0.583168 tig0016220:+351384 Msa1358170:CDS
TTCGGATGCAGCTTCACCTG+TGG 0.586183 tig0016220:+352559 Msa1358170:CDS
CAGTTCTTGCAGGAAGTCGA+CGG 0.588288 tig0016220:-354459 None:intergenic
GAGATTCTTGTGTTCATGTG+TGG 0.591639 tig0016220:-354094 None:intergenic
TACAACAGTGATACGTTCAA+AGG 0.595585 tig0016220:+352598 Msa1358170:CDS
GACTCCACCCAATGAAAACA+AGG 0.596227 tig0016220:+353161 Msa1358170:CDS
TCCACCACTTCTTTACGAAG+TGG 0.596303 tig0016220:-353670 None:intergenic
TCGAGTTGCATCACTGCGAA+TGG 0.597451 tig0016220:-353589 None:intergenic
AGTTCTAAAACACCAATTGG+TGG 0.601566 tig0016220:-353118 None:intergenic
GTGCATGAATCCTCTCACCC+TGG 0.601936 tig0016220:-354264 None:intergenic
TTGCAAGCAGAATTCAGCTA+CGG 0.601937 tig0016220:+353008 Msa1358170:CDS
GTACATATGATCTAGTTGAA+AGG 0.603113 tig0016220:+352123 Msa1358170:CDS
CAGCTACGGCTATGTCTGGA+TGG 0.603841 tig0016220:+353022 Msa1358170:CDS
ACACCCCGATGAAATGGATG+AGG 0.606414 tig0016220:+354132 Msa1358170:CDS
TATGATCAACTTAAAGTTAG+GGG 0.607138 tig0016220:+351338 None:intergenic
TGTGTGGAGGGTATCTTGGT+CGG 0.608323 tig0016220:-354078 None:intergenic
GCACCTTGACATATGCATCC+GGG 0.608916 tig0016220:-352703 None:intergenic
CACTATCCGTTGGAAAAGCG+CGG 0.610187 tig0016220:+351657 Msa1358170:CDS
ACTATCCGTTGGAAAAGCGC+GGG 0.610345 tig0016220:+351658 Msa1358170:CDS
ATGAAAACAAGGGATGGTAG+AGG 0.612015 tig0016220:+353172 Msa1358170:CDS
CTAGAACTCTAAACCCACAA+TGG 0.614323 tig0016220:+352765 Msa1358170:CDS
CATCAACATCAACAATCCAC+TGG 0.614787 tig0016220:+351903 Msa1358170:CDS
ACCACTTCGTAAAGAAGTGG+TGG 0.616825 tig0016220:+353669 Msa1358170:CDS
GAATGGTTTGTCTCGTGACA+AGG 0.617002 tig0016220:+351827 Msa1358170:CDS
TCATTGAACCCGTAGGTCCG+TGG 0.617562 tig0016220:-351803 None:intergenic
CACCCAATGAAAACAAGGGA+TGG 0.618631 tig0016220:+353166 Msa1358170:CDS
CATGAACACAAGAATCTCAC+AGG 0.619367 tig0016220:+354099 Msa1358170:CDS
GTTGGGTGAAGAACAAGCAA+TGG 0.619420 tig0016220:-353451 None:intergenic
AGTTGTCATGCACTATCCGT+TGG 0.619792 tig0016220:+351647 Msa1358170:CDS
ATAGTCCTTTGGCACCCGAA+TGG 0.620871 tig0016220:+352435 Msa1358170:CDS
TACCGCTGAACGCTGTGGAG+AGG 0.621333 tig0016220:+352897 Msa1358170:CDS
AATAATCATGCTGAAGTCCA+CGG 0.626372 tig0016220:+351786 Msa1358170:CDS
CCTGCCACAGTCCTCACAGT+AGG 0.628716 tig0016220:+353310 Msa1358170:CDS
GCCACCACTTCGTAAAGAAG+TGG 0.629915 tig0016220:+353666 Msa1358170:CDS
TTTGACAATTCCCAAATCAG+TGG 0.630492 tig0016220:+353337 Msa1358170:CDS
ATCCTGACCTTGTAAGAATG+CGG 0.634955 tig0016220:+354182 Msa1358170:CDS
AATACAACGAGCAATACACT+TGG 0.635555 tig0016220:+353275 Msa1358170:CDS
AGCACAAGACTTGATTCCAA+CGG 0.636163 tig0016220:+352667 Msa1358170:CDS
CCACCGCAAAACAGTTATGG+AGG 0.639525 tig0016220:+353095 Msa1358170:CDS
AGTCGACGGAAGAAGTTGAT+CGG 0.641660 tig0016220:-354445 None:intergenic
TATCATACCGCTGAACGCTG+TGG 0.642625 tig0016220:+352892 Msa1358170:CDS
ATCGAAGTAAAGTTCTACAA+AGG 0.644114 tig0016220:-351418 None:intergenic
ATGAGTATCTGCATAGCCAG+TGG 0.645735 tig0016220:-351919 None:intergenic
AAAAGATGAGATCATTGGGA+GGG 0.647144 tig0016220:+352868 Msa1358170:CDS
CAAAGACCTGAAAATTGGGA+AGG 0.649275 tig0016220:+353375 Msa1358170:CDS
TCACCAAGCAGTAAACATAG+TGG 0.653759 tig0016220:+353621 Msa1358170:CDS
GTCACAAATGGGTAAGAACA+AGG 0.654611 tig0016220:+353227 Msa1358170:CDS
GGTGCCAAAGGACTATCTGG+TGG 0.654724 tig0016220:-352428 None:intergenic
TCGACTTGACGACAAGAAAG+GGG 0.658551 tig0016220:+352460 Msa1358170:CDS
GAACGCTGTGGAGAGGCGTG+CGG 0.658744 tig0016220:+352904 Msa1358170:CDS
CCATTCATCATTGAACCCGT+AGG 0.661796 tig0016220:-351810 None:intergenic
TGAGAAACAACACATGAACA+AGG 0.662150 tig0016220:-353840 None:intergenic
GTGATGAAATGTGTGTCTTG+AGG 0.667671 tig0016220:-351856 None:intergenic
CTACGGCTATGTCTGGATGG+AGG 0.669332 tig0016220:+353025 Msa1358170:CDS
GTGAGAATTGGCAATTACAG+AGG 0.669944 tig0016220:+352230 Msa1358170:CDS
TTTCCCGGATGCATATGTCA+AGG 0.671093 tig0016220:+352700 Msa1358170:CDS
CACCAGAGAAAACTGTCATG+AGG 0.672325 tig0016220:-353756 None:intergenic
GAAACAAGTCCGTTTCTCGG+TGG 0.679691 tig0016220:+352053 Msa1358170:CDS
GTATGATCGTTTGAGGAGCG+TGG 0.680386 tig0016220:+354204 Msa1358170:CDS
ACACGAACGTACCACAACCT+CGG 0.680879 tig0016220:-352632 None:intergenic
GATGATGGTTTGTGTTAGGG+AGG 0.686083 tig0016220:-351878 None:intergenic
ATACAACGAGCAATACACTT+GGG 0.691267 tig0016220:+353276 Msa1358170:CDS
TATCGACTCTTGAAACAGGA+CGG 0.694274 tig0016220:+353410 Msa1358170:CDS
CAACGAGCAATACACTTGGG+AGG 0.698127 tig0016220:+353279 Msa1358170:CDS
GAAAAGATGAGATCATTGGG+AGG 0.698157 tig0016220:+352867 Msa1358170:CDS
ACTCCACCCAATGAAAACAA+GGG 0.702014 tig0016220:+353162 Msa1358170:CDS
GACACCTACTGTGAGGACTG+TGG 0.709482 tig0016220:-353314 None:intergenic
CAAAGGTCTATCATGCTCCG+AGG 0.711392 tig0016220:+352615 Msa1358170:CDS
ATGAATAAAGAAGTAGCACG+CGG 0.714106 tig0016220:-354304 None:intergenic
CAGTAAACATAGTGGCAGCG+CGG 0.720769 tig0016220:+353629 Msa1358170:CDS
TGTCAAAGACACCTACTGTG+AGG 0.734255 tig0016220:-353321 None:intergenic
ATAAGAATCAGAATCCGGAG+TGG 0.742906 tig0016220:+352270 Msa1358170:CDS
ATTCCTCATCCATTTCATCG+GGG 0.747292 tig0016220:-354135 None:intergenic
GGTTAGGTGATATCTGCATG+TGG 0.750592 tig0016220:+353797 Msa1358170:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAAGATCCTTTTCTTTAA+TGG - tig0016220:351463-351482 None:intergenic 20.0%
!!! TATCAGTGATGTAAATTTTA+AGG - tig0016220:351713-351732 None:intergenic 20.0%
!! AAATGACATAGTTTAGAAAT+AGG - tig0016220:353912-353931 None:intergenic 20.0%
!! TATGCAATCATGTATAAATA+GGG - tig0016220:353975-353994 None:intergenic 20.0%
!! ATATGCAATCATGTATAAAT+AGG - tig0016220:353976-353995 None:intergenic 20.0%
!!! ATTTGGAATTGAGGATTTTA+TGG - tig0016220:351742-351761 None:intergenic 25.0%
! AGTATTACATGATCTTCAAA+AGG - tig0016220:352821-352840 None:intergenic 25.0%
! TGAAGATCATGTAATACTTT+CGG + tig0016220:352823-352842 Msa1358170:CDS 25.0%
! ATAGAATCATTCATTCTAGA+TGG + tig0016220:352930-352949 Msa1358170:CDS 25.0%
! ACATATTGTGTAGCAAAATA+CGG + tig0016220:353205-353224 Msa1358170:CDS 25.0%
! CCTGATATTGAAAATAAAGT+TGG - tig0016220:354056-354075 None:intergenic 25.0%
!!! CCAACTTTATTTTCAATATC+AGG + tig0016220:354053-354072 Msa1358170:intron 25.0%
!! ATATGTTACTCCTTTTCAAA+TGG + tig0016220:354357-354376 Msa1358170:CDS 25.0%
ATCGAAGTAAAGTTCTACAA+AGG - tig0016220:351421-351440 None:intergenic 30.0%
TTTGTAGAACTTTACTTCGA+TGG + tig0016220:351420-351439 Msa1358170:CDS 30.0%
! CCTTTTCTTTAATGGTTGTT+CGG - tig0016220:351455-351474 None:intergenic 30.0%
AAAAGGATCTTAATCCTGTT+TGG + tig0016220:351469-351488 Msa1358170:CDS 30.0%
ACTAACTCAAGTTCATTTCT+TGG + tig0016220:351579-351598 Msa1358170:CDS 30.0%
ACTTCAGCATGATTATTTGT+TGG - tig0016220:351783-351802 None:intergenic 30.0%
ATTTCATCAGCTTCATACTT+CGG - tig0016220:351951-351970 None:intergenic 30.0%
TCTTTAAGTGCAAAATCAAC+CGG - tig0016220:352035-352054 None:intergenic 30.0%
AACTAGATCATATGTACTTG+CGG - tig0016220:352120-352139 None:intergenic 30.0%
GTACATATGATCTAGTTGAA+AGG + tig0016220:352123-352142 Msa1358170:CDS 30.0%
!! GATGTACTTTTTGTATGTCA+GGG + tig0016220:352145-352164 Msa1358170:CDS 30.0%
CTGATTCTTATCATAGTGTT+TGG - tig0016220:352261-352280 None:intergenic 30.0%
CTATGATAAGAATCAGAATC+CGG + tig0016220:352265-352284 Msa1358170:CDS 30.0%
! TCAGGTTTTTGCATTTTCAA+AGG + tig0016220:352295-352314 Msa1358170:CDS 30.0%
! CTCATCAAAGATGATTTTGT+AGG + tig0016220:352365-352384 Msa1358170:CDS 30.0%
!! ATGATTTTGTAGGTATTGTG+AGG + tig0016220:352375-352394 Msa1358170:CDS 30.0%
AAAGATCTTCATTCCATTGT+GGG - tig0016220:352781-352800 None:intergenic 30.0%
AAAAGATCTTCATTCCATTG+TGG - tig0016220:352782-352801 None:intergenic 30.0%
ACAACAAAGACCTGAAAATT+GGG + tig0016220:353371-353390 Msa1358170:CDS 30.0%
! AATAGGTAGAGAAAAAGTGT+AGG - tig0016220:353895-353914 None:intergenic 30.0%
AAATTCCTCATCCATTTCAT+CGG - tig0016220:354140-354159 None:intergenic 30.0%
! TAATGGTTGTTCGGAACTTT+TGG - tig0016220:351446-351465 None:intergenic 35.0%
CCGAACAACCATTAAAGAAA+AGG + tig0016220:351452-351471 Msa1358170:CDS 35.0%
AAGAGTGAGATAGTGTAGAT+TGG - tig0016220:351529-351548 None:intergenic 35.0%
!! TTGAGTTAGTAGCTTTGGAA+TGG - tig0016220:351569-351588 None:intergenic 35.0%
TGAACTTGAGTTAGTAGCTT+TGG - tig0016220:351574-351593 None:intergenic 35.0%
! GGAATTGAGGATTTTATGGT+TGG - tig0016220:351738-351757 None:intergenic 35.0%
! AACAGAAGGATTTGGAATTG+AGG - tig0016220:351751-351770 None:intergenic 35.0%
! AGCATGATTATTTGTTGGCA+AGG - tig0016220:351778-351797 None:intergenic 35.0%
AATAATCATGCTGAAGTCCA+CGG + tig0016220:351786-351805 Msa1358170:CDS 35.0%
AAAGAAACAAGTCCGTTTCT+CGG + tig0016220:352050-352069 Msa1358170:CDS 35.0%
!! GGATGTACTTTTTGTATGTC+AGG + tig0016220:352144-352163 Msa1358170:CDS 35.0%
TTCCTTCTATGGATCTTACT+GGG + tig0016220:352186-352205 Msa1358170:CDS 35.0%
AAGAAAGGGGAGAAAGTTAA+AGG + tig0016220:352473-352492 Msa1358170:CDS 35.0%
TACAACAGTGATACGTTCAA+AGG + tig0016220:352598-352617 Msa1358170:CDS 35.0%
TACATGATCTTCAAAAGGCT+CGG - tig0016220:352816-352835 None:intergenic 35.0%
TGGGAAAAGATGAGATCATT+GGG + tig0016220:352864-352883 Msa1358170:CDS 35.0%
AAAAGATGAGATCATTGGGA+GGG + tig0016220:352868-352887 Msa1358170:CDS 35.0%
TCATTCTAGATGGTTCAACT+TGG + tig0016220:352940-352959 Msa1358170:CDS 35.0%
!! AGTTCTAAAACACCAATTGG+TGG - tig0016220:353121-353140 None:intergenic 35.0%
!!! CCAATTGGTGTTTTAGAACT+CGG + tig0016220:353121-353140 Msa1358170:CDS 35.0%
!!! TACCATCCCTTGTTTTCATT+GGG - tig0016220:353171-353190 None:intergenic 35.0%
TAGCAAAATACGGTCACAAA+TGG + tig0016220:353215-353234 Msa1358170:CDS 35.0%
AGCAAAATACGGTCACAAAT+GGG + tig0016220:353216-353235 Msa1358170:CDS 35.0%
! GTGTATTGCTCGTTGTATTT+TGG - tig0016220:353274-353293 None:intergenic 35.0%
AATACAACGAGCAATACACT+TGG + tig0016220:353275-353294 Msa1358170:CDS 35.0%
ATACAACGAGCAATACACTT+GGG + tig0016220:353276-353295 Msa1358170:CDS 35.0%
TTTGACAATTCCCAAATCAG+TGG + tig0016220:353337-353356 Msa1358170:CDS 35.0%
CACAACAAAGACCTGAAAAT+TGG + tig0016220:353370-353389 Msa1358170:CDS 35.0%
! CCATTTTCTTAACACCAGTT+GGG - tig0016220:353471-353490 None:intergenic 35.0%
! CCAACTGGTGTTAAGAAAAT+GGG + tig0016220:353469-353488 Msa1358170:CDS 35.0%
!!! GGAGAAGTTGTTTTGAGTAT+AGG - tig0016220:353549-353568 None:intergenic 35.0%
TTTGCAGTTGGAAAATGGTT+AGG + tig0016220:353781-353800 Msa1358170:CDS 35.0%
TGAACAAGGACTGTTGTAAT+AGG - tig0016220:353829-353848 None:intergenic 35.0%
TGAGAAACAACACATGAACA+AGG - tig0016220:353843-353862 None:intergenic 35.0%
!! TCATGTGTTGTTTCTCATGT+TGG + tig0016220:353846-353865 Msa1358170:intron 35.0%
AATTCCTCATCCATTTCATC+GGG - tig0016220:354139-354158 None:intergenic 35.0%
ATGAATAAAGAAGTAGCACG+CGG - tig0016220:354307-354326 None:intergenic 35.0%
! TTTCTGTTTGTTAGCTGCTT+TGG + tig0016220:354330-354349 Msa1358170:CDS 35.0%
! TGTTACTCCTTTTCAAATGG+TGG + tig0016220:354360-354379 Msa1358170:CDS 35.0%
TAGCAGGTTTCTATTTCATG+AGG + tig0016220:354389-354408 Msa1358170:CDS 35.0%
AACATACTATCAGTTCTTGC+AGG - tig0016220:354472-354491 None:intergenic 35.0%
CTTAAAGTTAGGGGTAGATG+TGG + tig0016220:351347-351366 Msa1358170:CDS 40.0%
CACAATCTACTACCCAAAGA+TGG + tig0016220:351378-351397 Msa1358170:CDS 40.0%
TAGAAGCTCTCATTCCAAAC+AGG - tig0016220:351486-351505 None:intergenic 40.0%
GTGAGATAGTGTAGATTGGA+AGG - tig0016220:351525-351544 None:intergenic 40.0%
!! TAGTAGCTTTGGAATGGCAA+TGG - tig0016220:351563-351582 None:intergenic 40.0%
CTTGGTAAAGTTAGACTCAC+AGG + tig0016220:351597-351616 Msa1358170:CDS 40.0%
AAGGACTCAACAGAAGGATT+TGG - tig0016220:351759-351778 None:intergenic 40.0%
GTGATGAAATGTGTGTCTTG+AGG - tig0016220:351859-351878 None:intergenic 40.0%
TCCCTAACACAAACCATCAT+CGG + tig0016220:351880-351899 Msa1358170:CDS 40.0%
CATCAACATCAACAATCCAC+TGG + tig0016220:351903-351922 Msa1358170:CDS 40.0%
! TGTACTAGCTTCATAGGTTG+TGG - tig0016220:351987-352006 None:intergenic 40.0%
! TGCATGTGTACTAGCTTCAT+AGG - tig0016220:351993-352012 None:intergenic 40.0%
CTCAGTTACATCACTACAAC+CGG + tig0016220:352013-352032 Msa1358170:CDS 40.0%
TTTGTATGTCAGGGTCGTTA+AGG + tig0016220:352154-352173 Msa1358170:CDS 40.0%
CTTCCTTCTATGGATCTTAC+TGG + tig0016220:352185-352204 Msa1358170:CDS 40.0%
! GAGTCTTGATCCATTTGTTG+AGG + tig0016220:352208-352227 Msa1358170:CDS 40.0%
CCAATTCTCACCTCAACAAA+TGG - tig0016220:352221-352240 None:intergenic 40.0%
! CCATTTGTTGAGGTGAGAAT+TGG + tig0016220:352218-352237 Msa1358170:CDS 40.0%
GTGAGAATTGGCAATTACAG+AGG + tig0016220:352230-352249 Msa1358170:CDS 40.0%
ATAAGAATCAGAATCCGGAG+TGG + tig0016220:352270-352289 Msa1358170:CDS 40.0%
TGTACTGGAAGTTGTGATCA+AGG + tig0016220:352334-352353 Msa1358170:CDS 40.0%
CTATCTGGTGGAACTCTTAA+AGG - tig0016220:352419-352438 None:intergenic 40.0%
!! TATCGACTTGACGACAAGAA+AGG + tig0016220:352458-352477 Msa1358170:CDS 40.0%
!! ATCGACTTGACGACAAGAAA+GGG + tig0016220:352459-352478 Msa1358170:CDS 40.0%
TTGATGCTAGCTGTTTGGAT+TGG + tig0016220:352500-352519 Msa1358170:CDS 40.0%
!! ATGAAGCGTTTTCTGATGCA+TGG + tig0016220:352534-352553 Msa1358170:CDS 40.0%
! GTTTTCTGATGCATGGCATT+CGG + tig0016220:352541-352560 Msa1358170:CDS 40.0%
AGCACAAGACTTGATTCCAA+CGG + tig0016220:352667-352686 Msa1358170:CDS 40.0%
!!! CTGTCTTTGTTTTCAGCACT+TGG - tig0016220:352739-352758 None:intergenic 40.0%
CTAGAACTCTAAACCCACAA+TGG + tig0016220:352765-352784 Msa1358170:CDS 40.0%
CTGGGAAAAGATGAGATCAT+TGG + tig0016220:352863-352882 Msa1358170:CDS 40.0%
GAAAAGATGAGATCATTGGG+AGG + tig0016220:352867-352886 Msa1358170:CDS 40.0%
TTGCAAGCAGAATTCAGCTA+CGG + tig0016220:353008-353027 Msa1358170:CDS 40.0%
!! CCGAGTTCTAAAACACCAAT+TGG - tig0016220:353124-353143 None:intergenic 40.0%
ACTCCACCCAATGAAAACAA+GGG + tig0016220:353162-353181 Msa1358170:CDS 40.0%
!!! CTACCATCCCTTGTTTTCAT+TGG - tig0016220:353172-353191 None:intergenic 40.0%
ATGAAAACAAGGGATGGTAG+AGG + tig0016220:353172-353191 Msa1358170:CDS 40.0%
GTCACAAATGGGTAAGAACA+AGG + tig0016220:353227-353246 Msa1358170:CDS 40.0%
TTCCCAAATCAGTGGTGAAA+AGG + tig0016220:353345-353364 Msa1358170:CDS 40.0%
TCCCAAATCAGTGGTGAAAA+GGG + tig0016220:353346-353365 Msa1358170:CDS 40.0%
!!! ACCCTTTTCACCACTGATTT+GGG - tig0016220:353350-353369 None:intergenic 40.0%
CAAAGACCTGAAAATTGGGA+AGG + tig0016220:353375-353394 Msa1358170:CDS 40.0%
! TTCTCACCTTCCCAATTTTC+AGG - tig0016220:353384-353403 None:intergenic 40.0%
!! TATCGACTCTTGAAACAGGA+CGG + tig0016220:353410-353429 Msa1358170:CDS 40.0%
! CCCAACTGGTGTTAAGAAAA+TGG + tig0016220:353468-353487 Msa1358170:CDS 40.0%
! CCCATTTTCTTAACACCAGT+TGG - tig0016220:353472-353491 None:intergenic 40.0%
TCCCGAAAATGCATTACGTT+AGG + tig0016220:353566-353585 Msa1358170:CDS 40.0%
! GCCTAACGTAATGCATTTTC+GGG - tig0016220:353570-353589 None:intergenic 40.0%
! GGCCTAACGTAATGCATTTT+CGG - tig0016220:353571-353590 None:intergenic 40.0%
TCACCAAGCAGTAAACATAG+TGG + tig0016220:353621-353640 Msa1358170:CDS 40.0%
!! CAGATGTTGACTCACATCTA+TGG + tig0016220:353701-353720 Msa1358170:CDS 40.0%
GAAAGAAGTTTGCCTTGCTA+CGG - tig0016220:353735-353754 None:intergenic 40.0%
GTGTGTTTGCAGTTGGAAAA+TGG + tig0016220:353776-353795 Msa1358170:CDS 40.0%
GTGTAGGCAAAATCAACTCT+GGG - tig0016220:353879-353898 None:intergenic 40.0%
AGTGTAGGCAAAATCAACTC+TGG - tig0016220:353880-353899 None:intergenic 40.0%
TTCTTGTGTTCATGTGTGGA+GGG - tig0016220:354093-354112 None:intergenic 40.0%
ATTCTTGTGTTCATGTGTGG+AGG - tig0016220:354094-354113 None:intergenic 40.0%
GAGATTCTTGTGTTCATGTG+TGG - tig0016220:354097-354116 None:intergenic 40.0%
CATGAACACAAGAATCTCAC+AGG + tig0016220:354099-354118 Msa1358170:CDS 40.0%
ATTCCTCATCCATTTCATCG+GGG - tig0016220:354138-354157 None:intergenic 40.0%
! AGGTCAGGATTTTTGCTTGT+TGG - tig0016220:354172-354191 None:intergenic 40.0%
ATCCTGACCTTGTAAGAATG+CGG + tig0016220:354182-354201 Msa1358170:CDS 40.0%
GATCATACCGCATTCTTACA+AGG - tig0016220:354192-354211 None:intergenic 40.0%
GGTAGAATACAAACGGTTGT+AGG + tig0016220:354229-354248 Msa1358170:CDS 40.0%
ACAAACGGTTGTAGGTGATT+TGG + tig0016220:354237-354256 Msa1358170:CDS 40.0%
AAACCAGCCACCATTTGAAA+AGG - tig0016220:354370-354389 None:intergenic 40.0%
AAGTTAGGGGTAGATGTGGT+TGG + tig0016220:351351-351370 Msa1358170:CDS 45.0%
CTACTACCCAAAGATGGAGA+AGG + tig0016220:351384-351403 Msa1358170:CDS 45.0%
GAAGAGCCTTCTCCATCTTT+GGG - tig0016220:351393-351412 None:intergenic 45.0%
AGAAGAGCCTTCTCCATCTT+TGG - tig0016220:351394-351413 None:intergenic 45.0%
TGACAACTGCATCAGCTTGA+GGG - tig0016220:351635-351654 None:intergenic 45.0%
ATGACAACTGCATCAGCTTG+AGG - tig0016220:351636-351655 None:intergenic 45.0%
AGTTGTCATGCACTATCCGT+TGG + tig0016220:351647-351666 Msa1358170:CDS 45.0%
! AAAATCCCGCGCTTTTCCAA+CGG - tig0016220:351666-351685 None:intergenic 45.0%
CCATTCATCATTGAACCCGT+AGG - tig0016220:351813-351832 None:intergenic 45.0%
CCTACGGGTTCAATGATGAA+TGG + tig0016220:351810-351829 Msa1358170:CDS 45.0%
! GAATGGTTTGTCTCGTGACA+AGG + tig0016220:351827-351846 Msa1358170:CDS 45.0%
! GATGATGGTTTGTGTTAGGG+AGG - tig0016220:351881-351900 None:intergenic 45.0%
! GCCGATGATGGTTTGTGTTA+GGG - tig0016220:351884-351903 None:intergenic 45.0%
! TGCCGATGATGGTTTGTGTT+AGG - tig0016220:351885-351904 None:intergenic 45.0%
GTTGATGTTGATGCCGATGA+TGG - tig0016220:351896-351915 None:intergenic 45.0%
ATGAGTATCTGCATAGCCAG+TGG - tig0016220:351922-351941 None:intergenic 45.0%
CTCCCAGTAAGATCCATAGA+AGG - tig0016220:352191-352210 None:intergenic 45.0%
GAGAATGCAAGCATCTGTAC+TGG + tig0016220:352319-352338 Msa1358170:CDS 45.0%
AGTTCCACCAGATAGTCCTT+TGG + tig0016220:352424-352443 Msa1358170:CDS 45.0%
TCGTCAAGTCGATACCATTC+GGG - tig0016220:352452-352471 None:intergenic 45.0%
GTCGTCAAGTCGATACCATT+CGG - tig0016220:352453-352472 None:intergenic 45.0%
! TCGACTTGACGACAAGAAAG+GGG + tig0016220:352460-352479 Msa1358170:CDS 45.0%
!! GTGAGTTGATGCTAGCTGTT+TGG + tig0016220:352495-352514 Msa1358170:CDS 45.0%
CGTATCACTGTTGTAGTAGC+AGG - tig0016220:352593-352612 None:intergenic 45.0%
AACGGAGAAAAACCGTTTCC+CGG + tig0016220:352685-352704 Msa1358170:CDS 45.0%
TGACATATGCATCCGGGAAA+CGG - tig0016220:352700-352719 None:intergenic 45.0%
TTTCCCGGATGCATATGTCA+AGG + tig0016220:352700-352719 Msa1358170:CDS 45.0%
TGCACCTTGACATATGCATC+CGG - tig0016220:352707-352726 None:intergenic 45.0%
GCATATGTCAAGGTGCAGAT+AGG + tig0016220:352710-352729 Msa1358170:CDS 45.0%
! TGTGGGTTTAGAGTTCTAGC+AGG - tig0016220:352764-352783 None:intergenic 45.0%
CTTTCGGTTGAAGATCGAGT+TGG + tig0016220:352839-352858 Msa1358170:CDS 45.0%
!! GTTGAAGATCGAGTTGGTCT+GGG + tig0016220:352845-352864 Msa1358170:CDS 45.0%
TGATCAACATCCACAGCAAC+AGG - tig0016220:352971-352990 None:intergenic 45.0%
AATTCAGCTACGGCTATGTC+TGG + tig0016220:353018-353037 Msa1358170:CDS 45.0%
! TCCATAACTGTTTTGCGGTG+GGG - tig0016220:353096-353115 None:intergenic 45.0%
! CTCCATAACTGTTTTGCGGT+GGG - tig0016220:353097-353116 None:intergenic 45.0%
! TGGCCTCCATAACTGTTTTG+CGG - tig0016220:353101-353120 None:intergenic 45.0%
GACTCCACCCAATGAAAACA+AGG + tig0016220:353161-353180 Msa1358170:CDS 45.0%
!!! CATCCCTTGTTTTCATTGGG+TGG - tig0016220:353168-353187 None:intergenic 45.0%
CACCCAATGAAAACAAGGGA+TGG + tig0016220:353166-353185 Msa1358170:CDS 45.0%
TGTCAAAGACACCTACTGTG+AGG - tig0016220:353324-353343 None:intergenic 45.0%
!! GACCCTTTTCACCACTGATT+TGG - tig0016220:353351-353370 None:intergenic 45.0%
!! CGCATATCGACTCTTGAAAC+AGG + tig0016220:353406-353425 Msa1358170:CDS 45.0%
GTTGGGTGAAGAACAAGCAA+TGG - tig0016220:353454-353473 None:intergenic 45.0%
TTGCTTGTTCTTCACCCAAC+TGG + tig0016220:353454-353473 Msa1358170:CDS 45.0%
GAAAATGGGCGAACTTCACT+TGG + tig0016220:353483-353502 Msa1358170:CDS 45.0%
CTGCCACTATGTTTACTGCT+TGG - tig0016220:353627-353646 None:intergenic 45.0%
ACCACTTCTTTACGAAGTGG+TGG - tig0016220:353670-353689 None:intergenic 45.0%
ACCACTTCGTAAAGAAGTGG+TGG + tig0016220:353669-353688 Msa1358170:CDS 45.0%
TCCACCACTTCTTTACGAAG+TGG - tig0016220:353673-353692 None:intergenic 45.0%
CACCAGAGAAAACTGTCATG+AGG - tig0016220:353759-353778 None:intergenic 45.0%
TTCTCTGGTGTGTTTGCAGT+TGG + tig0016220:353769-353788 Msa1358170:CDS 45.0%
GGTTAGGTGATATCTGCATG+TGG + tig0016220:353797-353816 Msa1358170:CDS 45.0%
!! TTCATGTGTGGAGGGTATCT+TGG - tig0016220:354085-354104 None:intergenic 45.0%
TGTAGTACACCCCGATGAAA+TGG + tig0016220:354126-354145 Msa1358170:CDS 45.0%
TACCGCATTCTTACAAGGTC+AGG - tig0016220:354187-354206 None:intergenic 45.0%
GAATGCGGTATGATCGTTTG+AGG + tig0016220:354197-354216 Msa1358170:CDS 45.0%
CGTGGCTGGTAGAATACAAA+CGG + tig0016220:354222-354241 Msa1358170:CDS 45.0%
! ACTCCTTTTCAAATGGTGGC+TGG + tig0016220:354364-354383 Msa1358170:CDS 45.0%
! AAGAAGTTGATCGGTGCAGA+CGG - tig0016220:354439-354458 None:intergenic 45.0%
! AGTCGACGGAAGAAGTTGAT+CGG - tig0016220:354448-354467 None:intergenic 45.0%
CACTATCCGTTGGAAAAGCG+CGG + tig0016220:351657-351676 Msa1358170:CDS 50.0%
! ACTATCCGTTGGAAAAGCGC+GGG + tig0016220:351658-351677 Msa1358170:CDS 50.0%
! GGGATTTTCTCTCGCGTAAG+AGG + tig0016220:351678-351697 Msa1358170:CDS 50.0%
GTTGGCAAGGACTCAACAGA+AGG - tig0016220:351765-351784 None:intergenic 50.0%
GAAACAAGTCCGTTTCTCGG+TGG + tig0016220:352053-352072 Msa1358170:CDS 50.0%
GCAAAAACCTGATGCCACTC+CGG - tig0016220:352287-352306 None:intergenic 50.0%
ATAGTCCTTTGGCACCCGAA+TGG + tig0016220:352435-352454 Msa1358170:CDS 50.0%
CAAAGGTCTATCATGCTCCG+AGG + tig0016220:352615-352634 Msa1358170:CDS 50.0%
TCTATCATGCTCCGAGGTTG+TGG + tig0016220:352621-352640 Msa1358170:CDS 50.0%
ACACGAACGTACCACAACCT+CGG - tig0016220:352635-352654 None:intergenic 50.0%
! GGGAAACGGTTTTTCTCCGT+TGG - tig0016220:352686-352705 None:intergenic 50.0%
GCACCTTGACATATGCATCC+GGG - tig0016220:352706-352725 None:intergenic 50.0%
!! GGTTGAAGATCGAGTTGGTC+TGG + tig0016220:352844-352863 Msa1358170:CDS 50.0%
TATCATACCGCTGAACGCTG+TGG + tig0016220:352892-352911 Msa1358170:CDS 50.0%
CTTGGAAAAGCCTGTTGCTG+TGG + tig0016220:352958-352977 Msa1358170:CDS 50.0%
! CCTCCATAACTGTTTTGCGG+TGG - tig0016220:353098-353117 None:intergenic 50.0%
CCACCGCAAAACAGTTATGG+AGG + tig0016220:353095-353114 Msa1358170:CDS 50.0%
CAGTTATGGAGGCCACCAAT+TGG + tig0016220:353106-353125 Msa1358170:CDS 50.0%
!! CTCGGAGTGCTGAATGCAAT+AGG + tig0016220:353139-353158 Msa1358170:CDS 50.0%
CAACGAGCAATACACTTGGG+AGG + tig0016220:353279-353298 Msa1358170:CDS 50.0%
! TCGAGTTGCATCACTGCGAA+TGG - tig0016220:353592-353611 None:intergenic 50.0%
CAGTAAACATAGTGGCAGCG+CGG + tig0016220:353629-353648 Msa1358170:CDS 50.0%
GCCACCACTTCGTAAAGAAG+TGG + tig0016220:353666-353685 Msa1358170:CDS 50.0%
! CGCCTCATGACAGTTTTCTC+TGG + tig0016220:353754-353773 Msa1358170:CDS 50.0%
!! TGTGTGGAGGGTATCTTGGT+CGG - tig0016220:354081-354100 None:intergenic 50.0%
ACACCCCGATGAAATGGATG+AGG + tig0016220:354132-354151 Msa1358170:CDS 50.0%
GTATGATCGTTTGAGGAGCG+TGG + tig0016220:354204-354223 Msa1358170:CDS 50.0%
! TGTAGGTGATTTGGCTAGCC+AGG + tig0016220:354246-354265 Msa1358170:CDS 50.0%
! GTAGGTGATTTGGCTAGCCA+GGG + tig0016220:354247-354266 Msa1358170:CDS 50.0%
GGATTCATGCACTGTTGAGC+TGG + tig0016220:354275-354294 Msa1358170:CDS 50.0%
!! CAAATGGTGGCTGGTTTAGC+AGG + tig0016220:354373-354392 Msa1358170:CDS 50.0%
GGAAGCCTGTGTCGAAATCT+CGG - tig0016220:354418-354437 None:intergenic 50.0%
CAGTTCTTGCAGGAAGTCGA+CGG - tig0016220:354462-354481 None:intergenic 50.0%
TCTCGCGTAAGAGGTGAGCT+TGG + tig0016220:351687-351706 Msa1358170:CDS 55.0%
TGCTGAAGTCCACGGACCTA+CGG + tig0016220:351794-351813 Msa1358170:CDS 55.0%
TCATTGAACCCGTAGGTCCG+TGG - tig0016220:351806-351825 None:intergenic 55.0%
ACAAGTCCGTTTCTCGGTGG+AGG + tig0016220:352056-352075 Msa1358170:CDS 55.0%
TCCGTTTCTCGGTGGAGGAA+GGG + tig0016220:352061-352080 Msa1358170:CDS 55.0%
ACCCTTCCTCCACCGAGAAA+CGG - tig0016220:352065-352084 None:intergenic 55.0%
GGCTCGTGAGCTTCCTTCTA+TGG + tig0016220:352175-352194 Msa1358170:CDS 55.0%
TCAGAATCCGGAGTGGCATC+AGG + tig0016220:352277-352296 Msa1358170:CDS 55.0%
GGTGCCAAAGGACTATCTGG+TGG - tig0016220:352431-352450 None:intergenic 55.0%
TCGGGTGCCAAAGGACTATC+TGG - tig0016220:352434-352453 None:intergenic 55.0%
CGATACCATTCGGGTGCCAA+AGG - tig0016220:352443-352462 None:intergenic 55.0%
TTCGGATGCAGCTTCACCTG+TGG + tig0016220:352559-352578 Msa1358170:CDS 55.0%
CAGCTACGGCTATGTCTGGA+TGG + tig0016220:353022-353041 Msa1358170:CDS 55.0%
CTACGGCTATGTCTGGATGG+AGG + tig0016220:353025-353044 Msa1358170:CDS 55.0%
GCCCCACCGCAAAACAGTTA+TGG + tig0016220:353092-353111 Msa1358170:CDS 55.0%
GACACCTACTGTGAGGACTG+TGG - tig0016220:353317-353336 None:intergenic 55.0%
! ATTTGGCTAGCCAGGGTGAG+AGG + tig0016220:354254-354273 Msa1358170:CDS 55.0%
GTGCATGAATCCTCTCACCC+TGG - tig0016220:354267-354286 None:intergenic 55.0%
GGCATCCGAGATTTCGACAC+AGG + tig0016220:354410-354429 Msa1358170:CDS 55.0%
GCTGAAGTCCACGGACCTAC+GGG + tig0016220:351795-351814 Msa1358170:CDS 60.0%
GTCCGTTTCTCGGTGGAGGA+AGG + tig0016220:352060-352079 Msa1358170:CDS 60.0%
CTCGGTGGAGGAAGGGTAGT+TGG + tig0016220:352068-352087 Msa1358170:CDS 60.0%
GGTGGAGGAAGGGTAGTTGG+TGG + tig0016220:352071-352090 Msa1358170:CDS 60.0%
TACCGCTGAACGCTGTGGAG+AGG + tig0016220:352897-352916 Msa1358170:CDS 60.0%
CCTACTGTGAGGACTGTGGC+AGG - tig0016220:353313-353332 None:intergenic 60.0%
CCTGCCACAGTCCTCACAGT+AGG + tig0016220:353310-353329 Msa1358170:CDS 60.0%
AACATAGTGGCAGCGCGGCT+CGG + tig0016220:353634-353653 Msa1358170:CDS 60.0%
ATGGAGCATGCGCCGTAGCA+AGG + tig0016220:353720-353739 Msa1358170:CDS 60.0%
! GATCGTTTGAGGAGCGTGGC+TGG + tig0016220:354208-354227 Msa1358170:CDS 60.0%
GTAGCAGGCGTGCTGTCCAC+AGG - tig0016220:352578-352597 None:intergenic 65.0%
CGCCTCTCCACAGCGTTCAG+CGG - tig0016220:352902-352921 None:intergenic 65.0%
! GAACGCTGTGGAGAGGCGTG+CGG + tig0016220:352904-352923 Msa1358170:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0016220 gene 351339 354495 351339 ID=Msa1358170;Name=Msa1358170
tig0016220 mRNA 351339 354495 351339 ID=Msa1358170-mRNA-1;Parent=Msa1358170;Name=Msa1358170-mRNA-1;_AED=0.04;_eAED=0.04;_QI=0|0|0|1|1|1|2|0|981
tig0016220 exon 351339 353863 351339 ID=Msa1358170-mRNA-1:exon:10653;Parent=Msa1358170-mRNA-1
tig0016220 exon 354075 354495 354075 ID=Msa1358170-mRNA-1:exon:10654;Parent=Msa1358170-mRNA-1
tig0016220 CDS 351339 353863 351339 ID=Msa1358170-mRNA-1:cds;Parent=Msa1358170-mRNA-1
tig0016220 CDS 354075 354495 354075 ID=Msa1358170-mRNA-1:cds;Parent=Msa1358170-mRNA-1
Gene Sequence

>Msa1358170

ATGATCAACTTAAAGTTAGGGGTAGATGTGGTTGGTGCTCACAATCTACTACCCAAAGATGGAGAAGGCTCTTCTAATGCCTTTGTAGAACTTTACTTCGATGGCCAAAAGTTCCGAACAACCATTAAAGAAAAGGATCTTAATCCTGTTTGGAATGAGAGCTTCTACTTCAACATATCAGATCCTTCCAATCTACACTATCTCACTCTTGAAGCTTATGTCCATTGCCATTCCAAAGCTACTAACTCAAGTTCATTTCTTGGTAAAGTTAGACTCACAGGTACTTCTTTTGTCCCTCAAGCTGATGCAGTTGTCATGCACTATCCGTTGGAAAAGCGCGGGATTTTCTCTCGCGTAAGAGGTGAGCTTGGCCTTAAAATTTACATCACTGATAATCCAACCATAAAATCCTCAATTCCAAATCCTTCTGTTGAGTCCTTGCCAACAAATAATCATGCTGAAGTCCACGGACCTACGGGTTCAATGATGAATGGTTTGTCTCGTGACAAGGTTGAGTCCTCAAGACACACATTTCATCACCTCCCTAACACAAACCATCATCGGCATCAACATCAACAATCCACTGGCTATGCAGATACTCATTATGTACCGAAGTATGAAGCTGATGAAATGAAAGCTGATCAACCACAACCTATGAAGCTAGTACACATGCACTCAGTTACATCACTACAACCGGTTGATTTTGCACTTAAAGAAACAAGTCCGTTTCTCGGTGGAGGAAGGGTAGTTGGTGGTCGCGTTGTTCATAAAGACAAGACCGCAAGTACATATGATCTAGTTGAAAGGATGTACTTTTTGTATGTCAGGGTCGTTAAGGCTCGTGAGCTTCCTTCTATGGATCTTACTGGGAGTCTTGATCCATTTGTTGAGGTGAGAATTGGCAATTACAGAGGTATTACCAAACACTATGATAAGAATCAGAATCCGGAGTGGCATCAGGTTTTTGCATTTTCAAAGGAGAGAATGCAAGCATCTGTACTGGAAGTTGTGATCAAGGACAAAGATCTCATCAAAGATGATTTTGTAGGTATTGTGAGGTTTGACATCAATGAGATTCCTTTAAGAGTTCCACCAGATAGTCCTTTGGCACCCGAATGGTATCGACTTGACGACAAGAAAGGGGAGAAAGTTAAAGGTGAGTTGATGCTAGCTGTTTGGATTGGTACTCAAGCAGATGAAGCGTTTTCTGATGCATGGCATTCGGATGCAGCTTCACCTGTGGACAGCACGCCTGCTACTACAACAGTGATACGTTCAAAGGTCTATCATGCTCCGAGGTTGTGGTACGTTCGTGTCAATGTTGTAGAAGCACAAGACTTGATTCCAACGGAGAAAAACCGTTTCCCGGATGCATATGTCAAGGTGCAGATAGGAAACCAAGTGCTGAAAACAAAGACAGTTCCTGCTAGAACTCTAAACCCACAATGGAATGAAGATCTTTTGTTTGTTGCTGCCGAGCCTTTTGAAGATCATGTAATACTTTCGGTTGAAGATCGAGTTGGTCTGGGAAAAGATGAGATCATTGGGAGGGTTATCATACCGCTGAACGCTGTGGAGAGGCGTGCGGATGATAGAATCATTCATTCTAGATGGTTCAACTTGGAAAAGCCTGTTGCTGTGGATGTTGATCAGCTGAAGAGAGAAAAGTTTGCAAGCAGAATTCAGCTACGGCTATGTCTGGATGGAGGATATCATGTTCTTGATGAATCAACTCATTACAGCAGCGATCTACGCCCCACCGCAAAACAGTTATGGAGGCCACCAATTGGTGTTTTAGAACTCGGAGTGCTGAATGCAATAGGACTCCACCCAATGAAAACAAGGGATGGTAGAGGCACTTCTGATACATATTGTGTAGCAAAATACGGTCACAAATGGGTAAGAACAAGGACACTTGTTGACAACTTGTCGCCAAAATACAACGAGCAATACACTTGGGAGGTCTTTGATCCTGCCACAGTCCTCACAGTAGGTGTCTTTGACAATTCCCAAATCAGTGGTGAAAAGGGTCACAACAAAGACCTGAAAATTGGGAAGGTGAGAATTCGCATATCGACTCTTGAAACAGGACGGATTTACACACACTCATATCCATTGCTTGTTCTTCACCCAACTGGTGTTAAGAAAATGGGCGAACTTCACTTGGCAATAAGATTCTCATGCACTTCTTTTGCAAACATGCTTTACCTATACTCAAAACAACTTCTCCCGAAAATGCATTACGTTAGGCCATTCGCAGTGATGCAACTCGATATGCTACGTCACCAAGCAGTAAACATAGTGGCAGCGCGGCTCGGTCGAGCAGAGCCACCACTTCGTAAAGAAGTGGTGGAATACATGTCAGATGTTGACTCACATCTATGGAGCATGCGCCGTAGCAAGGCAAACTTCTTTCGCCTCATGACAGTTTTCTCTGGTGTGTTTGCAGTTGGAAAATGGTTAGGTGATATCTGCATGTGGTTAAATCCTATTACAACAGTCCTTGTTCATGTGTTGTTTCTCATGTACCGACCAAGATACCCTCCACACATGAACACAAGAATCTCACAGGCTGATGTAGTACACCCCGATGAAATGGATGAGGAATTTGATACTTTTCCAACAAGCAAAAATCCTGACCTTGTAAGAATGCGGTATGATCGTTTGAGGAGCGTGGCTGGTAGAATACAAACGGTTGTAGGTGATTTGGCTAGCCAGGGTGAGAGGATTCATGCACTGTTGAGCTGGAGAGATCCGCGTGCTACTTCTTTATTCATTACTTTCTGTTTGTTAGCTGCTTTGGTGTTATATGTTACTCCTTTTCAAATGGTGGCTGGTTTAGCAGGTTTCTATTTCATGAGGCATCCGAGATTTCGACACAGGCTTCCGTCTGCACCGATCAACTTCTTCCGTCGACTTCCTGCAAGAACTGATAGTATGTTGTAG
Protein sequence

>Msa1358170

MINLKLGVDVVGAHNLLPKDGEGSSNAFVELYFDGQKFRTTIKEKDLNPVWNESFYFNISDPSNLHYLTLEAYVHCHSKATNSSSFLGKVRLTGTSFVPQADAVVMHYPLEKRGIFSRVRGELGLKIYITDNPTIKSSIPNPSVESLPTNNHAEVHGPTGSMMNGLSRDKVESSRHTFHHLPNTNHHRHQHQQSTGYADTHYVPKYEADEMKADQPQPMKLVHMHSVTSLQPVDFALKETSPFLGGGRVVGGRVVHKDKTASTYDLVERMYFLYVRVVKARELPSMDLTGSLDPFVEVRIGNYRGITKHYDKNQNPEWHQVFAFSKERMQASVLEVVIKDKDLIKDDFVGIVRFDINEIPLRVPPDSPLAPEWYRLDDKKGEKVKGELMLAVWIGTQADEAFSDAWHSDAASPVDSTPATTTVIRSKVYHAPRLWYVRVNVVEAQDLIPTEKNRFPDAYVKVQIGNQVLKTKTVPARTLNPQWNEDLLFVAAEPFEDHVILSVEDRVGLGKDEIIGRVIIPLNAVERRADDRIIHSRWFNLEKPVAVDVDQLKREKFASRIQLRLCLDGGYHVLDESTHYSSDLRPTAKQLWRPPIGVLELGVLNAIGLHPMKTRDGRGTSDTYCVAKYGHKWVRTRTLVDNLSPKYNEQYTWEVFDPATVLTVGVFDNSQISGEKGHNKDLKIGKVRIRISTLETGRIYTHSYPLLVLHPTGVKKMGELHLAIRFSCTSFANMLYLYSKQLLPKMHYVRPFAVMQLDMLRHQAVNIVAARLGRAEPPLRKEVVEYMSDVDSHLWSMRRSKANFFRLMTVFSGVFAVGKWLGDICMWLNPITTVLVHVLFLMYRPRYPPHMNTRISQADVVHPDEMDEEFDTFPTSKNPDLVRMRYDRLRSVAGRIQTVVGDLASQGERIHALLSWRDPRATSLFITFCLLAALVLYVTPFQMVAGLAGFYFMRHPRFRHRLPSAPINFFRRLPARTDSML*