AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1362250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1362250 MtrunA17_Chr3g0138281 97.644 382 8 1 1 382 1 381 0.0 766
Msa1362250 MtrunA17_Chr4g0065141 58.497 306 123 3 76 378 79 383 4.47e-127 370
Msa1362250 MtrunA17_Chr5g0407161 54.575 306 133 4 74 378 77 377 1.66e-112 333
Msa1362250 MtrunA17_Chr3g0144111 54.577 284 104 4 95 378 1 259 1.08e-99 296
Msa1362250 MtrunA17_Chr1g0194191 45.402 348 160 8 49 378 50 385 1.37e-89 275
Msa1362250 MtrunA17_Chr2g0293811 43.491 338 174 7 55 379 34 367 2.91e-85 263
Msa1362250 MtrunA17_Chr1g0150451 36.162 271 147 8 80 346 75 323 1.80e-40 146
Msa1362250 MtrunA17_Chr1g0150071 29.714 350 177 8 61 357 5 338 2.85e-40 146
Msa1362250 MtrunA17_Chr1g0171571 35.106 282 148 9 77 344 382 642 3.08e-40 150
Msa1362250 MtrunA17_Chr3g0136811 29.944 354 175 9 59 357 3 338 7.12e-40 145
Msa1362250 MtrunA17_Chr3g0136711 35.055 271 150 8 80 346 32 280 1.24e-38 140
Msa1362250 MtrunA17_Chr4g0001761 30.530 321 156 7 81 347 22 329 1.45e-38 141
Msa1362250 MtrunA17_Chr5g0430481 35.926 270 138 7 90 348 131 376 1.76e-38 141
Msa1362250 MtrunA17_Chr6g0483411 31.392 309 170 8 66 348 28 320 1.21e-37 138
Msa1362250 MtrunA17_Chr3g0105371 36.090 266 141 7 90 347 95 339 1.22e-37 138
Msa1362250 MtrunA17_Chr4g0068971 45.679 162 76 4 224 378 110 266 1.71e-37 136
Msa1362250 MtrunA17_Chr3g0105351 35.821 268 139 8 90 347 71 315 3.85e-37 136
Msa1362250 MtrunA17_Chr1g0212961 35.227 264 143 8 90 350 103 341 8.18e-37 136
Msa1362250 MtrunA17_Chr3g0115851 35.983 239 134 5 114 347 58 282 2.90e-36 133
Msa1362250 MtrunA17_Chr5g0426141 35.271 258 139 8 95 347 48 282 1.60e-35 131
Msa1362250 MtrunA17_Chr3g0126511 32.632 285 165 7 69 349 82 343 2.81e-35 132
Msa1362250 MtrunA17_Chr2g0279151 36.797 231 127 6 122 347 65 281 1.41e-34 129
Msa1362250 MtrunA17_Chr4g0050381 35.065 231 131 5 122 347 61 277 2.17e-32 123
Msa1362250 MtrunA17_Chr2g0317021 34.167 240 139 6 113 347 108 333 3.28e-31 121
Msa1362250 MtrunA17_Chr2g0299981 29.467 319 160 9 89 349 227 538 7.44e-30 120
Msa1362250 MtrunA17_Chr3g0127091 27.152 302 171 7 79 343 57 346 8.57e-29 115
Msa1362250 MtrunA17_Chr1g0150781 29.870 308 160 10 90 349 247 546 9.10e-29 117
Msa1362250 MtrunA17_Chr8g0360351 33.195 241 140 7 113 347 57 282 5.45e-28 111
Msa1362250 MtrunA17_Chr4g0070871 29.836 305 159 9 84 344 208 501 8.63e-28 114
Msa1362250 MtrunA17_Chr3g0112291 32.184 261 135 7 119 344 120 373 9.17e-28 112
Msa1362250 MtrunA17_Chr4g0051661 33.824 272 147 10 83 346 151 397 5.55e-27 110
Msa1362250 MtrunA17_Chr1g0158781 33.333 285 151 9 81 344 111 377 2.06e-26 108
Msa1362250 MtrunA17_Chr3g0135961 29.710 276 151 7 110 349 274 542 2.65e-26 110
Msa1362250 MtrunA17_Chr5g0396731 33.981 206 118 4 122 316 107 305 2.32e-25 105
Msa1362250 MtrunA17_Chr8g0389921 28.617 311 174 8 84 363 150 443 6.40e-25 105
Msa1362250 MtrunA17_Chr5g0435721 34.112 214 112 6 122 316 223 426 2.33e-24 104
Msa1362250 MtrunA17_Chr7g0263281 27.778 288 147 12 118 349 66 348 2.22e-18 85.9
Msa1362250 MtrunA17_Chr2g0295401 28.938 273 139 13 124 350 94 357 2.58e-18 85.5
Msa1362250 MtrunA17_Chr1g0211251 27.915 283 143 12 118 344 76 353 6.46e-18 84.7
Msa1362250 MtrunA17_Chr4g0066371 29.434 265 133 11 122 344 86 338 1.01e-17 84.0
Msa1362250 MtrunA17_Chr4g0067711 27.097 310 169 13 92 350 53 356 1.02e-17 83.6
Msa1362250 MtrunA17_Chr3g0089721 29.075 227 121 6 122 311 68 291 1.07e-17 83.6
Msa1362250 MtrunA17_Chr3g0089711 28.384 229 124 6 120 311 66 291 2.66e-17 82.4
Msa1362250 MtrunA17_Chr7g0256751 27.490 251 141 8 122 337 69 313 4.16e-17 82.4
Msa1362250 MtrunA17_Chr8g0371321 26.333 300 156 13 118 361 74 364 5.70e-17 81.6
Msa1362250 MtrunA17_Chr1g0151781 30.045 223 109 8 121 303 67 282 5.82e-17 81.3
Msa1362250 MtrunA17_Chr7g0250061 26.351 296 154 12 121 361 75 361 6.84e-17 81.6
Msa1362250 MtrunA17_Chr1g0213351 26.481 287 149 12 121 351 79 359 1.05e-16 80.9
Msa1362250 MtrunA17_Chr3g0133801 27.881 269 152 9 117 360 59 310 3.09e-16 79.7
Msa1362250 MtrunA17_Chr1g0192421 28.400 250 129 10 122 331 89 328 6.93e-16 78.2
Msa1362250 MtrunA17_Chr1g0181901 28.395 243 121 11 118 319 71 301 4.30e-15 76.3
Msa1362250 MtrunA17_Chr2g0294291 28.627 255 141 10 114 332 66 315 8.44e-15 75.1
Msa1362250 MtrunA17_Chr5g0394181 29.218 243 147 10 122 346 362 597 1.06e-14 75.5
Msa1362250 MtrunA17_Chr7g0272061 28.195 266 127 11 122 334 91 345 3.83e-14 73.2
Msa1362250 MtrunA17_Chr7g0249421 35.417 144 69 4 183 308 206 343 4.46e-14 73.6
Msa1362250 MtrunA17_Chr7g0256771 28.634 227 120 7 122 310 70 292 5.22e-14 72.4
Msa1362250 MtrunA17_Chr7g0243571 27.208 283 146 10 122 350 92 368 1.35e-13 72.0
Msa1362250 MtrunA17_Chr7g0238501 26.667 225 126 7 121 306 262 486 3.39e-13 70.9
Msa1362250 MtrunA17_Chr7g0221691 35.172 145 70 5 183 309 231 369 8.50e-13 69.3
Msa1362250 MtrunA17_Chr1g0209451 29.278 263 137 13 121 357 67 306 2.38e-12 68.2
Msa1362250 MtrunA17_Chr7g0225501 30.698 215 108 12 117 303 79 280 3.87e-11 64.3
Msa1362250 MtrunA17_Chr4g0004971 26.991 226 113 8 120 300 76 294 4.49e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1362250 AT2G25620.1 66.244 394 117 7 1 381 1 391 0.0 510
Msa1362250 AT2G25620.2 66.244 394 117 7 1 381 1 391 0.0 510
Msa1362250 AT2G33700.1 55.556 306 131 4 74 378 76 377 2.05e-114 338
Msa1362250 AT3G51470.1 52.581 310 138 3 71 380 61 361 1.15e-109 326
Msa1362250 AT3G51470.2 52.581 310 138 3 71 380 17 317 1.19e-109 324
Msa1362250 AT3G62260.1 48.734 316 145 6 76 378 71 382 5.52e-90 276
Msa1362250 AT3G62260.2 48.734 316 145 6 76 378 72 383 6.55e-90 276
Msa1362250 AT1G48040.1 46.006 313 156 5 75 377 71 380 1.41e-83 259
Msa1362250 AT3G62260.3 48.485 297 136 6 95 378 1 293 1.93e-82 254
Msa1362250 AT3G17250.1 42.902 317 163 6 75 378 110 421 1.08e-74 238
Msa1362250 AT5G24940.1 36.531 271 146 8 80 346 31 279 3.23e-42 153
Msa1362250 AT5G10740.1 37.269 271 144 8 80 346 31 279 6.36e-42 150
Msa1362250 AT5G10740.2 37.269 271 144 8 80 346 31 279 6.36e-42 150
Msa1362250 AT4G31750.1 36.531 271 146 8 80 346 31 279 6.22e-40 144
Msa1362250 AT4G31750.3 36.531 271 146 8 80 346 31 279 6.22e-40 144
Msa1362250 AT4G31750.2 36.531 271 146 8 80 346 31 279 6.65e-40 144
Msa1362250 AT2G25070.1 28.895 353 179 9 61 358 5 340 6.75e-40 145
Msa1362250 AT2G25070.2 28.895 353 179 9 61 358 5 340 6.75e-40 145
Msa1362250 AT5G53140.1 33.456 272 155 7 81 348 100 349 1.20e-39 146
Msa1362250 AT5G53140.2 33.456 272 155 7 81 348 100 349 1.20e-39 146
Msa1362250 AT4G31860.1 29.969 327 161 7 80 351 21 334 5.16e-39 143
Msa1362250 AT1G78200.5 35.498 231 133 3 122 347 19 238 1.50e-37 135
Msa1362250 AT1G78200.4 35.498 231 133 3 122 347 19 238 1.50e-37 135
Msa1362250 AT2G20630.1 33.721 258 143 6 95 347 45 279 2.08e-37 136
Msa1362250 AT1G78200.1 35.498 231 133 3 122 347 64 283 3.66e-37 136
Msa1362250 AT1G78200.3 35.498 231 133 3 122 347 64 283 3.66e-37 136
Msa1362250 AT1G78200.2 35.498 231 133 3 122 347 64 283 3.66e-37 136
Msa1362250 AT2G20630.2 33.463 257 143 6 95 346 45 278 3.98e-37 136
Msa1362250 AT2G30020.1 36.431 269 140 9 90 348 147 394 8.35e-37 138
Msa1362250 AT4G28400.3 34.496 258 141 6 95 347 27 261 9.60e-37 134
Msa1362250 AT4G28400.1 34.496 258 141 6 95 347 49 283 1.92e-36 134
Msa1362250 AT4G31860.3 31.690 284 131 6 80 313 21 291 6.22e-36 133
Msa1362250 AT2G40860.4 32.404 287 158 9 75 347 287 551 7.70e-36 138
Msa1362250 AT2G40860.1 32.746 284 155 9 75 344 385 646 9.85e-36 138
Msa1362250 AT1G43900.1 34.066 273 151 9 80 347 121 369 2.20e-35 133
Msa1362250 AT1G18030.1 31.944 288 152 9 88 348 80 350 3.27e-35 132
Msa1362250 AT1G07160.1 35.338 266 140 8 90 344 130 374 9.61e-35 132
Msa1362250 AT1G22280.1 35.271 258 139 9 95 347 47 281 1.18e-33 127
Msa1362250 AT3G15260.2 34.855 241 136 6 113 347 64 289 1.20e-33 127
Msa1362250 AT3G15260.1 34.855 241 136 6 113 347 64 289 1.20e-33 127
Msa1362250 AT1G34750.2 34.468 235 134 6 116 344 59 279 6.70e-33 124
Msa1362250 AT1G34750.1 34.468 235 134 6 116 344 59 279 6.70e-33 124
Msa1362250 AT1G34750.3 34.468 235 134 6 116 344 59 279 6.70e-33 124
Msa1362250 AT1G67820.2 38.342 193 100 4 121 306 114 294 3.41e-32 125
Msa1362250 AT1G67820.1 38.342 193 100 4 121 306 150 330 4.14e-32 126
Msa1362250 AT2G40180.1 34.182 275 148 8 90 351 136 390 4.25e-32 125
Msa1362250 AT4G31860.2 30.970 268 122 6 80 297 21 275 2.05e-31 120
Msa1362250 AT1G22280.3 34.470 264 139 10 95 347 47 287 3.41e-31 120
Msa1362250 AT2G40860.2 31.387 274 152 9 75 334 385 636 5.46e-31 124
Msa1362250 AT2G29380.1 32.226 301 147 12 82 344 77 358 1.17e-28 115
Msa1362250 AT1G07430.1 31.890 254 144 8 74 310 114 355 1.40e-28 116
Msa1362250 AT4G26080.1 30.201 298 152 10 90 344 136 420 3.93e-28 114
Msa1362250 AT2G34740.1 31.801 261 146 9 95 348 101 336 6.32e-28 112
Msa1362250 AT5G51760.2 30.392 306 152 10 89 344 85 379 9.80e-28 113
Msa1362250 AT5G51760.1 30.392 306 152 10 89 344 115 409 1.36e-27 113
Msa1362250 AT5G59220.1 35.000 240 122 8 90 310 119 343 4.32e-27 111
Msa1362250 AT1G18030.2 31.933 238 119 8 88 299 80 300 4.43e-27 109
Msa1362250 AT1G72770.4 29.841 315 159 9 90 349 197 504 2.33e-26 110
Msa1362250 AT1G72770.1 29.841 315 159 9 90 349 197 504 2.33e-26 110
Msa1362250 AT1G72770.3 29.841 315 159 9 90 349 197 504 2.33e-26 110
Msa1362250 AT1G17550.1 28.615 325 163 8 84 349 190 504 2.88e-26 110
Msa1362250 AT1G17550.2 28.615 325 163 8 84 349 190 504 2.88e-26 110
Msa1362250 AT4G27800.1 27.019 322 178 8 81 361 58 363 3.09e-26 108
Msa1362250 AT3G11410.1 32.946 258 132 9 78 310 102 343 3.18e-26 108
Msa1362250 AT5G57050.1 29.373 303 153 11 90 344 120 409 3.75e-26 109
Msa1362250 AT5G57050.2 29.373 303 153 11 90 344 80 369 3.79e-26 108
Msa1362250 AT4G28400.2 34.286 210 114 5 95 299 49 239 6.39e-26 105
Msa1362250 AT1G34750.4 33.158 190 111 5 116 299 59 238 2.83e-22 95.1
Msa1362250 AT2G40860.3 31.897 232 121 9 75 290 385 595 1.32e-21 97.1
Msa1362250 AT2G34740.3 32.075 212 116 8 95 299 97 287 2.13e-21 94.0
Msa1362250 AT2G34740.2 32.075 212 116 8 95 299 115 305 2.27e-21 94.4
Msa1362250 AT4G08260.1 30.859 256 129 10 95 348 1 210 2.67e-21 91.7
Msa1362250 AT4G27800.2 26.531 294 159 8 81 333 58 335 2.19e-20 91.7
Msa1362250 AT4G27800.3 27.465 284 148 9 81 322 58 325 7.89e-20 89.7
Msa1362250 AT5G02760.1 31.780 236 113 11 121 319 71 295 1.54e-19 89.7
Msa1362250 AT5G59220.2 37.097 186 90 7 90 256 119 296 1.04e-18 86.3
Msa1362250 AT3G12620.2 28.975 283 140 13 118 344 78 355 2.76e-18 85.9
Msa1362250 AT3G12620.1 28.975 283 140 13 118 344 78 355 2.76e-18 85.9
Msa1362250 AT2G20050.2 28.625 269 130 9 122 344 143 395 2.78e-17 84.3
Msa1362250 AT2G20050.1 28.625 269 130 9 122 344 143 395 2.83e-17 84.3
Msa1362250 AT3G63340.8 29.496 278 122 12 149 366 198 461 8.72e-17 82.8
Msa1362250 AT3G63340.5 29.496 278 122 12 149 366 198 461 8.72e-17 82.8
Msa1362250 AT3G63340.1 29.496 278 122 12 149 366 198 461 8.72e-17 82.8
Msa1362250 AT3G63340.7 29.496 278 122 12 149 366 198 461 8.80e-17 82.8
Msa1362250 AT3G63340.6 29.496 278 122 12 149 366 198 461 9.69e-17 82.8
Msa1362250 AT3G63340.3 29.496 278 122 12 149 366 198 461 9.69e-17 82.8
Msa1362250 AT3G63340.10 29.496 278 122 12 149 366 198 461 9.69e-17 82.8
Msa1362250 AT3G63340.4 29.496 278 122 12 149 366 198 461 9.69e-17 82.8
Msa1362250 AT3G63340.11 29.496 278 122 12 149 366 198 461 1.04e-16 82.4
Msa1362250 AT5G01700.1 28.315 279 143 11 120 352 27 294 1.15e-16 80.9
Msa1362250 AT3G63340.12 29.496 278 122 12 149 366 198 461 1.33e-16 81.6
Msa1362250 AT4G33920.1 26.027 292 156 12 114 348 52 340 1.97e-16 80.5
Msa1362250 AT1G47380.1 29.104 268 144 10 117 357 57 305 2.42e-16 80.5
Msa1362250 AT5G01700.2 28.315 279 143 11 120 352 76 343 3.59e-16 79.7
Msa1362250 AT3G55050.1 27.273 286 147 13 118 347 79 359 6.17e-16 79.0
Msa1362250 AT3G55050.2 27.273 286 147 13 118 347 79 359 6.17e-16 79.0
Msa1362250 AT3G16800.2 31.556 225 112 9 121 310 92 309 6.91e-16 78.6
Msa1362250 AT3G16800.1 31.556 225 112 9 121 310 92 309 6.91e-16 78.6
Msa1362250 AT3G16800.3 31.556 225 112 9 121 310 92 309 6.91e-16 78.6
Msa1362250 AT3G16800.4 37.415 147 67 5 183 310 74 214 7.88e-16 77.0
Msa1362250 AT3G16800.6 37.415 147 67 5 183 310 74 214 7.88e-16 77.0
Msa1362250 AT3G05640.2 26.756 299 154 13 81 332 48 328 1.99e-15 77.4
Msa1362250 AT3G05640.1 26.756 299 154 13 81 332 48 328 1.99e-15 77.4
Msa1362250 AT3G05640.3 26.756 299 154 13 81 332 48 328 1.99e-15 77.4
Msa1362250 AT3G51370.1 25.088 283 151 11 118 344 74 351 2.31e-15 77.4
Msa1362250 AT3G51370.3 25.088 283 151 11 118 344 74 351 2.31e-15 77.4
Msa1362250 AT3G17090.1 29.032 248 131 9 121 331 83 322 3.32e-15 77.0
Msa1362250 AT3G17090.2 28.926 242 127 9 121 325 83 316 4.15e-15 75.9
Msa1362250 AT4G38520.2 25.795 283 153 11 118 346 76 355 4.30e-15 76.6
Msa1362250 AT4G38520.1 25.795 283 153 11 118 346 76 355 4.30e-15 76.6
Msa1362250 AT4G32950.1 28.641 206 118 6 121 303 71 270 8.21e-15 75.1
Msa1362250 AT3G63320.2 28.571 273 119 12 151 357 52 314 1.35e-14 75.1
Msa1362250 AT1G09160.2 30.315 254 132 11 117 344 61 295 1.53e-14 75.1
Msa1362250 AT1G09160.1 30.315 254 132 11 117 344 61 295 1.53e-14 75.1
Msa1362250 AT5G27930.1 27.600 250 115 11 107 308 82 313 2.39e-14 74.3
Msa1362250 AT5G27930.2 27.600 250 115 11 107 308 82 313 2.39e-14 74.3
Msa1362250 AT5G57050.3 30.256 195 104 8 90 258 120 308 2.97e-14 73.2
Msa1362250 AT3G63320.1 26.706 337 134 14 124 357 71 397 3.51e-14 73.9
Msa1362250 AT1G72770.2 31.220 205 103 6 90 256 197 401 5.43e-14 73.2
Msa1362250 AT1G72770.5 31.220 205 103 6 90 256 197 401 5.43e-14 73.2
Msa1362250 AT1G22280.2 35.616 146 82 5 95 240 47 180 1.18e-13 69.7
Msa1362250 AT5G66080.1 26.855 283 146 11 118 344 77 354 1.36e-13 72.0
Msa1362250 AT5G26010.1 26.493 268 148 10 124 353 75 331 2.64e-13 70.9
Msa1362250 AT3G63340.2 33.918 171 84 8 212 366 337 494 4.76e-13 71.2
Msa1362250 AT1G79630.6 32.275 189 91 8 183 346 111 287 7.78e-13 69.7
Msa1362250 AT1G79630.3 32.275 189 91 8 183 346 111 287 7.78e-13 69.7
Msa1362250 AT1G79630.4 32.275 189 91 8 183 346 111 287 7.78e-13 69.7
Msa1362250 AT1G79630.5 32.275 189 91 8 183 346 111 287 7.78e-13 69.7
Msa1362250 AT1G79630.2 32.292 192 93 8 183 349 151 330 9.25e-13 69.7
Msa1362250 AT1G79630.7 32.292 192 93 8 183 349 151 330 9.25e-13 69.7
Msa1362250 AT1G16220.1 29.515 227 119 10 148 346 161 374 1.26e-12 69.3
Msa1362250 AT1G79630.8 32.275 189 91 8 183 346 217 393 1.31e-12 69.3
Msa1362250 AT1G79630.1 32.275 189 91 8 183 346 221 397 1.38e-12 69.3
Msa1362250 AT5G06750.3 25.357 280 152 10 120 344 80 357 1.89e-12 68.6
Msa1362250 AT5G06750.1 25.357 280 152 10 120 344 80 357 1.89e-12 68.6
Msa1362250 AT5G19280.1 27.419 248 147 10 123 346 339 577 3.26e-12 68.6
Msa1362250 AT3G02750.2 34.507 142 69 5 183 306 215 350 5.05e-12 67.8
Msa1362250 AT3G02750.1 34.507 142 69 5 183 306 215 350 5.05e-12 67.8
Msa1362250 AT4G32950.2 28.141 199 114 6 121 296 71 263 1.04e-11 65.5
Msa1362250 AT3G16560.1 29.054 148 83 4 181 306 273 420 2.67e-11 65.5
Msa1362250 AT3G16560.4 29.054 148 83 4 181 306 273 420 2.67e-11 65.5
Msa1362250 AT3G16560.2 29.054 148 83 4 181 306 273 420 2.67e-11 65.5
Msa1362250 AT3G16560.3 29.054 148 83 4 181 306 273 420 2.68e-11 65.5

Find 127 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGTTACCAAAATTCGAAAA+TGG 0.203487 tig0017748:+9933 None:intergenic
GCAGCAGCCTCCACCAAATT+TGG 0.216972 tig0017748:-7643 Msa1362250:CDS
GTCGGTGGTCGGTGAGGGTT+TGG 0.228941 tig0017748:+10134 None:intergenic
TGAGTTTGCGCAAGTATTTA+TGG 0.229399 tig0017748:-7537 Msa1362250:three_prime_UTR
GCGCAAAATCTACAGCATTT+TGG 0.230172 tig0017748:+7778 None:intergenic
TCATAGGCTGCGATGGGATT+TGG 0.232647 tig0017748:-7815 Msa1362250:CDS
GCATTGGCTACTCTTGTTAT+AGG 0.258949 tig0017748:-8385 Msa1362250:CDS
CTTAATGTTGCTCGAGCTAT+TGG 0.303340 tig0017748:-7939 Msa1362250:CDS
CATAGGCTGCGATGGGATTT+GGG 0.327999 tig0017748:-7814 Msa1362250:CDS
GATGGACCGAGTGCTTTCTA+TGG 0.328546 tig0017748:-8988 Msa1362250:CDS
TATCGGGTTCCGGTCAAATA+TGG 0.330817 tig0017748:-9073 Msa1362250:CDS
AGTTTCTGACTGTAGGTTGC+TGG 0.335395 tig0017748:-8114 Msa1362250:intron
TCCGGAGGATGTGCTGATAT+CGG 0.336894 tig0017748:-9090 Msa1362250:CDS
GAGCGTGGTGCTACCAAATT+TGG 0.350164 tig0017748:+7630 None:intergenic
CTCAATAAACAACTTATAGA+TGG 0.363410 tig0017748:-9006 Msa1362250:CDS
GCAATGGATGGATTAGGATT+TGG 0.372864 tig0017748:+9807 None:intergenic
GTCTATGATGGATATCTTAA+CGG 0.377116 tig0017748:-7966 Msa1362250:CDS
CAGAGGCGTATCGAGGCATC+TGG 0.390804 tig0017748:-7996 Msa1362250:CDS
TGTTACTTGTTAGGTGTAGT+AGG 0.391570 tig0017748:-7419 Msa1362250:three_prime_UTR
CATCCTCCGGAACGGAGCTT+TGG 0.398949 tig0017748:+9102 None:intergenic
TCGGTGGTCGGTGAGGGTTT+GGG 0.400048 tig0017748:+10135 None:intergenic
CTTTCATGCAACCTGGTTTG+TGG 0.400398 tig0017748:+8021 None:intergenic
TTCCTTCCAAAGCTCCGTTC+CGG 0.404057 tig0017748:-9108 Msa1362250:CDS
GCAGAAGCAATGGATGGATT+AGG 0.405446 tig0017748:+9801 None:intergenic
CTCTGCTCTTTCATGCAACC+TGG 0.407237 tig0017748:+8014 None:intergenic
AACTGTAGGTGTTACTTGTT+AGG 0.413137 tig0017748:-7428 Msa1362250:intron
AAAAGAGTGTGTTGGGAAAG+AGG 0.414423 tig0017748:+10082 None:intergenic
ATGTGGAGATTTGTTTGAAA+GGG 0.417255 tig0017748:+10052 None:intergenic
TGAATCTCAATTCTCATAAG+AGG 0.424835 tig0017748:+7485 None:intergenic
CGGTGGTCGGTGGTCGGTGA+GGG 0.425084 tig0017748:+10129 None:intergenic
AGTTACCAAAATTCGAAAAT+GGG 0.428227 tig0017748:+9934 None:intergenic
TTTATATGTCGGCGATTCGA+AGG 0.437263 tig0017748:+10017 None:intergenic
TGACTGTAGGTTGCTGGTGG+TGG 0.438915 tig0017748:-8108 Msa1362250:intron
TGGCTTGGTCAGGTGTTTGA+TGG 0.441121 tig0017748:-8583 Msa1362250:intron
CCGGAGGATGTGCTGATATC+GGG 0.442854 tig0017748:-9089 Msa1362250:CDS
AGAGGCGTATCGAGGCATCT+GGG 0.450237 tig0017748:-7995 Msa1362250:CDS
CTCAATGCTGCCCTTGCATC+CGG 0.452615 tig0017748:-8415 Msa1362250:CDS
TTGCTGGTGGTGGCAAATGC+TGG 0.454788 tig0017748:-8098 Msa1362250:CDS
ATGCAGTGGTGCCGGATGCA+AGG 0.459353 tig0017748:+8404 None:intergenic
GCAAACAGACTATGCATTTG+CGG 0.463349 tig0017748:-8449 Msa1362250:CDS
TTTGAAGAAAAGAGTGTGTT+GGG 0.473612 tig0017748:+10075 None:intergenic
AATGTGGAGATTTGTTTGAA+AGG 0.474500 tig0017748:+10051 None:intergenic
TGCATCCGGCACCACTGCAT+TGG 0.476188 tig0017748:-8401 Msa1362250:CDS
TCTATGATGGATATCTTAAC+GGG 0.479521 tig0017748:-7965 Msa1362250:CDS
TGCAGTGGTGCCGGATGCAA+GGG 0.482277 tig0017748:+8405 None:intergenic
AGCTGTATGCAGTAAGGATC+TGG 0.483557 tig0017748:-7727 Msa1362250:CDS
GATGTGCTGATATCGGGTTC+CGG 0.494574 tig0017748:-9083 Msa1362250:CDS
CCCGATATCAGCACATCCTC+CGG 0.497125 tig0017748:+9089 None:intergenic
AGATCCTTACTGCATACAGC+TGG 0.500453 tig0017748:+7729 None:intergenic
TAAGTTGTTTATTGAGCCCA+TGG 0.501821 tig0017748:+9013 None:intergenic
TTATCCTCGAGAATGAACTT+TGG 0.503539 tig0017748:+8517 None:intergenic
AGTAGCCAATGCAGTGGTGC+CGG 0.504279 tig0017748:+8396 None:intergenic
ACTATCCTTTCAATATCCAC+TGG 0.504474 tig0017748:+8487 None:intergenic
CACAGGAGCTTCTCTGCTGA+AGG 0.507072 tig0017748:-7600 Msa1362250:CDS
AGGCGTATCGAGGCATCTGG+GGG 0.513077 tig0017748:-7993 Msa1362250:CDS
ATGGACCGAGTGCTTTCTAT+GGG 0.513585 tig0017748:-8987 Msa1362250:CDS
GCGGTGGTCGGTGGTCGGTG+AGG 0.520865 tig0017748:+10128 None:intergenic
AGAATGACCGTCGTAAATGA+AGG 0.524484 tig0017748:+10106 None:intergenic
ATGGATGGATTAGGATTTGG+AGG 0.527042 tig0017748:+9810 None:intergenic
TTTCTCATCATAGGCTGCGA+TGG 0.528638 tig0017748:-7822 Msa1362250:CDS
TATGTCGGCGATTCGAAGGG+TGG 0.528965 tig0017748:+10021 None:intergenic
TTCTCATCATAGGCTGCGAT+GGG 0.530891 tig0017748:-7821 Msa1362250:CDS
GAAGGAATGAAAGGCAAAGA+TGG 0.533012 tig0017748:-7903 Msa1362250:CDS
TGAGATTCATTTGTAACTGT+AGG 0.533426 tig0017748:-7471 Msa1362250:intron
TGCATAGTCTGTTTGCAGAA+AGG 0.536620 tig0017748:+8456 None:intergenic
TTATATGTCGGCGATTCGAA+GGG 0.537707 tig0017748:+10018 None:intergenic
CGACCCAGCTGTATGCAGTA+AGG 0.541516 tig0017748:-7733 Msa1362250:CDS
TAAATGAAGGCGGTGGTCGG+TGG 0.544330 tig0017748:+10119 None:intergenic
GTTTGAAGAAAAGAGTGTGT+TGG 0.546377 tig0017748:+10074 None:intergenic
TAAAATAAAATTAAACAAGA+AGG 0.548575 tig0017748:+7512 None:intergenic
ATTTCCCAGTGGATATTGAA+AGG 0.550709 tig0017748:-8492 Msa1362250:CDS
CTTCCAAAGCTCCGTTCCGG+AGG 0.555450 tig0017748:-9105 Msa1362250:CDS
CGTGGTGCTACCAAATTTGG+TGG 0.556204 tig0017748:+7633 None:intergenic
TGGCTACTCTTGTTATAGGA+AGG 0.559140 tig0017748:-8381 Msa1362250:intron
GAGGCGTATCGAGGCATCTG+GGG 0.559861 tig0017748:-7994 Msa1362250:CDS
CTATCCTTTCAATATCCACT+GGG 0.563023 tig0017748:+8488 None:intergenic
ATGTCAAGAGACCACAAACC+AGG 0.567267 tig0017748:-8032 Msa1362250:CDS
AGAGAAGCTCCTGTGCACTC+TGG 0.567952 tig0017748:+7608 None:intergenic
TGGCACATGGAAGGAATGAA+AGG 0.568858 tig0017748:-7912 Msa1362250:CDS
GGTCAGGTGTTTGATGGACA+CGG 0.571598 tig0017748:-8577 Msa1362250:intron
TTTCTGCATGGTAAAACAAG+AGG 0.572653 tig0017748:+9753 None:intergenic
ATTGGTGACTGGCACATGGA+AGG 0.574252 tig0017748:-7921 Msa1362250:CDS
CTCCGGAACGGAGCTTTGGA+AGG 0.575225 tig0017748:+9106 None:intergenic
TTCTGACTGTAGGTTGCTGG+TGG 0.579507 tig0017748:-8111 Msa1362250:intron
CGAAAAGCAGAAGCAATGGA+TGG 0.580021 tig0017748:+9795 None:intergenic
TCGTAAATGAAGGCGGTGGT+CGG 0.582325 tig0017748:+10116 None:intergenic
TTGCTCGAGCTATTGGTGAC+TGG 0.582496 tig0017748:-7932 Msa1362250:CDS
GAGGACGAATTTCTCATCAT+AGG 0.583587 tig0017748:-7831 Msa1362250:CDS
CTCCTGTGCACTCTGGAGCG+TGG 0.585430 tig0017748:+7615 None:intergenic
ACACATCCTCCATATTTGAC+CGG 0.585526 tig0017748:+9064 None:intergenic
AGACAATTTAGCTGTTGTTG+TGG 0.586656 tig0017748:-7676 Msa1362250:CDS
TTTGCCAAAGTTCATTCTCG+AGG 0.590449 tig0017748:-8521 Msa1362250:CDS
CAGTAAGGATCTGGTTGATG+AGG 0.591652 tig0017748:-7718 Msa1362250:CDS
TGAAGGCGGTGGTCGGTGGT+CGG 0.591726 tig0017748:+10123 None:intergenic
GAAGGGTGGTTTAACAAATG+TGG 0.593767 tig0017748:+10035 None:intergenic
TTGACGAAAAGCAGAAGCAA+TGG 0.595015 tig0017748:+9791 None:intergenic
CGAGCAGTGCTGTGCCGTCG+TGG 0.598079 tig0017748:-8068 Msa1362250:CDS
GGGTTTGGGTTTCGAAGATG+CGG 0.598198 tig0017748:+10149 None:intergenic
AGCTATTGGTGACTGGCACA+TGG 0.600561 tig0017748:-7925 Msa1362250:CDS
CACCACGCTCCAGAGTGCAC+AGG 0.602591 tig0017748:-7617 Msa1362250:CDS
TTTGGGTTTCGAAGATGCGG+TGG 0.614214 tig0017748:+10152 None:intergenic
GCTTTGGAAGGAAATCAGAC+TGG 0.619255 tig0017748:+9118 None:intergenic
GGTGCTACCAAATTTGGTGG+AGG 0.621044 tig0017748:+7636 None:intergenic
CGGGTTCCGGTCAAATATGG+AGG 0.621074 tig0017748:-9070 Msa1362250:CDS
GGAATGAAAGGCAAAGATGG+TGG 0.624005 tig0017748:-7900 Msa1362250:CDS
AGTTCAGGCTCTGCACTGAG+TGG 0.625933 tig0017748:+7876 None:intergenic
GACTACAAAACTTACTGCCG+AGG 0.627191 tig0017748:-7850 Msa1362250:CDS
TATGATGAGAAATTCGTCCT+CGG 0.637476 tig0017748:+7833 None:intergenic
GATCCTTACTGCATACAGCT+GGG 0.642529 tig0017748:+7730 None:intergenic
GGTGTGAATTGTTTCAGTGA+AGG 0.642841 tig0017748:-9855 Msa1362250:CDS
ACCGTCGTAAATGAAGGCGG+TGG 0.644318 tig0017748:+10112 None:intergenic
TATCAGCACATCCTCCGGAA+CGG 0.646071 tig0017748:+9094 None:intergenic
GAAAGAGCAGAGGCGTATCG+AGG 0.646196 tig0017748:-8003 Msa1362250:CDS
TCTGTTGAGGATGTTACTGA+AGG 0.648815 tig0017748:-9144 Msa1362250:CDS
CTTTCTGACTGTATCATATG+CGG 0.655064 tig0017748:-7385 Msa1362250:three_prime_UTR
TGAAAACAAATCATAAAACA+AGG 0.663165 tig0017748:-10192 Msa1362250:five_prime_UTR
CAGGTTGCATGAAAGAGCAG+AGG 0.671998 tig0017748:-8013 Msa1362250:CDS
TATACCCCATAGAAAGCACT+CGG 0.672295 tig0017748:+8982 None:intergenic
AACAAGAGTAGCCAATGCAG+TGG 0.673761 tig0017748:+8390 None:intergenic
GTTGTCTGATGCATCTGTTG+AGG 0.696072 tig0017748:-9157 Msa1362250:CDS
GGCGTATCGAGGCATCTGGG+GGG 0.697326 tig0017748:-7992 Msa1362250:CDS
ACCACCGCCTTCATTTACGA+CGG 0.704422 tig0017748:-10113 Msa1362250:five_prime_UTR
GGTTTCTTACAAGAGAAGCG+TGG 0.710252 tig0017748:+9718 None:intergenic
CGAGGATAAAGATTTCCCAG+TGG 0.737162 tig0017748:-8503 Msa1362250:CDS
TGGACCGAGTGCTTTCTATG+GGG 0.737710 tig0017748:-8986 Msa1362250:intron
ATGACCGTCGTAAATGAAGG+CGG 0.742021 tig0017748:+10109 None:intergenic
CAGGTGTTTGATGGACACGG+CGG 0.806389 tig0017748:-8574 Msa1362250:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTGATATTCAATAATTT+GGG - tig0017748:9195-9214 Msa1362250:intron 10.0%
!! TAAAATAAAATTAAACAAGA+AGG + tig0017748:10039-10058 None:intergenic 10.0%
!! ATTTAATGTAGATAAAAACT+AGG - tig0017748:8062-8081 Msa1362250:CDS 15.0%
!!! GATTTTGATATTCAATAATT+TGG - tig0017748:9194-9213 Msa1362250:intron 15.0%
!! GCTAATTTATAGTAAAAAAA+AGG + tig0017748:9228-9247 None:intergenic 15.0%
!!! TTTATGATTTGTTTTCACAA+CGG + tig0017748:7353-7372 None:intergenic 20.0%
!! TGAAAACAAATCATAAAACA+AGG - tig0017748:7356-7375 Msa1362250:three_prime_UTR 20.0%
!!! ATTAGGTCTTTTTTATATGT+CGG + tig0017748:7545-7564 None:intergenic 20.0%
!!! TCAGAAAAAGTGTAAAAATT+AGG + tig0017748:7562-7581 None:intergenic 20.0%
!! CAAAAGAAAAAACAAAAGAT+CGG + tig0017748:7854-7873 None:intergenic 20.0%
!!! ATAATGATGTTACTTAGTTT+TGG - tig0017748:7902-7921 Msa1362250:CDS 20.0%
!! GAAATGAAATGAAATAATGA+TGG - tig0017748:8118-8137 Msa1362250:intron 20.0%
!!! TAATTGTTGATTATGTTTTG+TGG - tig0017748:8200-8219 Msa1362250:intron 20.0%
!! ATAATCAATAATCAATCCTA+AGG + tig0017748:8239-8258 None:intergenic 20.0%
!!! TAGGATTGATTATTGATTAT+TGG - tig0017748:8239-8258 Msa1362250:intron 20.0%
!! TGATATGATAGTTGTTAAAA+TGG - tig0017748:8620-8639 Msa1362250:intron 20.0%
!! GATATGATAGTTGTTAAAAT+GGG - tig0017748:8621-8640 Msa1362250:intron 20.0%
!!! TGCTAATCTTATTTTGTAAT+TGG - tig0017748:8777-8796 Msa1362250:intron 20.0%
!!! TTTTTTTTTGAACAAACTGT+AGG - tig0017748:10106-10125 Msa1362250:five_prime_UTR 20.0%
!! TTTAACCCATTTTCGAATTT+TGG - tig0017748:7609-7628 Msa1362250:CDS 25.0%
! AGTTACCAAAATTCGAAAAT+GGG + tig0017748:7617-7636 None:intergenic 25.0%
!!! ATTTTCGAATTTTGGTAACT+GGG - tig0017748:7617-7636 Msa1362250:CDS 25.0%
!!! TTTTTGAAGTTTGGATCTTA+TGG - tig0017748:7646-7665 Msa1362250:CDS 25.0%
! GATCTTATGGTTAAGAAAAA+TGG - tig0017748:7659-7678 Msa1362250:CDS 25.0%
! AGAAAAATGGAAGAAATTGA+AGG - tig0017748:7672-7691 Msa1362250:CDS 25.0%
! TGACAAATGGAAAGAAAAAA+AGG + tig0017748:8000-8019 None:intergenic 25.0%
! AGAATGTGTAATTTAAGCTT+TGG - tig0017748:8033-8052 Msa1362250:CDS 25.0%
! ATGAAATGAAATAATGATGG+CGG - tig0017748:8121-8140 Msa1362250:intron 25.0%
!!! TATTGATTATTGGTGTTAGT+AGG - tig0017748:8249-8268 Msa1362250:intron 25.0%
!!! ATTGATTATTGGTGTTAGTA+GGG - tig0017748:8250-8269 Msa1362250:intron 25.0%
!!! ATGTTTGTGTTGACAATTTT+AGG - tig0017748:8507-8526 Msa1362250:CDS 25.0%
! CTCAATAAACAACTTATAGA+TGG - tig0017748:8542-8561 Msa1362250:CDS 25.0%
! TACAAAATAAGATTAGCATG+TGG + tig0017748:8775-8794 None:intergenic 25.0%
! TAATATGTGTTTATGCACAT+TGG + tig0017748:8826-8845 None:intergenic 25.0%
! TTACTATAAATTAGCACCAA+AGG - tig0017748:9233-9252 Msa1362250:intron 25.0%
!!! CATCTGACATTTTAATTTAC+TGG - tig0017748:10186-10205 Msa1362250:five_prime_UTR 25.0%
! TTTGAAGAAAAGAGTGTGTT+GGG + tig0017748:7476-7495 None:intergenic 30.0%
! ATGTGGAGATTTGTTTGAAA+GGG + tig0017748:7499-7518 None:intergenic 30.0%
! AATGTGGAGATTTGTTTGAA+AGG + tig0017748:7500-7519 None:intergenic 30.0%
CCAGATTGTAAAAAGCAATT+GGG + tig0017748:7588-7607 None:intergenic 30.0%
!!! CCAATTGCTTTTTACAATCT+GGG - tig0017748:7586-7605 Msa1362250:CDS 30.0%
CAGTTACCAAAATTCGAAAA+TGG + tig0017748:7618-7637 None:intergenic 30.0%
!! CATTTTCGAATTTTGGTAAC+TGG - tig0017748:7616-7635 Msa1362250:CDS 30.0%
AAAACTAGGACTAGAATAGA+AGG - tig0017748:8076-8095 Msa1362250:CDS 30.0%
TGAAATGAAATAATGATGGC+GGG - tig0017748:8122-8141 Msa1362250:intron 30.0%
AAAAGAGAATAACGACACAT+GGG + tig0017748:8675-8694 None:intergenic 30.0%
AATGAAAGAAGACAAAACGA+AGG + tig0017748:8698-8717 None:intergenic 30.0%
AAAAACTCCATAGCTAGTAA+AGG + tig0017748:8855-8874 None:intergenic 30.0%
CAGTTGACAAGAATAGATAT+AGG - tig0017748:8876-8895 Msa1362250:intron 30.0%
AAGTAACGTTATATGTCGAA+TGG - tig0017748:8945-8964 Msa1362250:intron 30.0%
CAAGAAATTTAGCATACCTT+TGG + tig0017748:9252-9271 None:intergenic 30.0%
AAAAGACATAAATCTGACTG+AGG - tig0017748:9294-9313 Msa1362250:intron 30.0%
GAGCATGAAAATGGAAATAT+GGG + tig0017748:9353-9372 None:intergenic 30.0%
AGAGCATGAAAATGGAAATA+TGG + tig0017748:9354-9373 None:intergenic 30.0%
!! GTCTATGATGGATATCTTAA+CGG - tig0017748:9582-9601 Msa1362250:intron 30.0%
!! TCTATGATGGATATCTTAAC+GGG - tig0017748:9583-9602 Msa1362250:intron 30.0%
TGAATCTCAATTCTCATAAG+AGG + tig0017748:10066-10085 None:intergenic 30.0%
TGAGATTCATTTGTAACTGT+AGG - tig0017748:10077-10096 Msa1362250:five_prime_UTR 30.0%
! GTTTGAAGAAAAGAGTGTGT+TGG + tig0017748:7477-7496 None:intergenic 35.0%
!!! CCCAATTGCTTTTTACAATC+TGG - tig0017748:7585-7604 Msa1362250:CDS 35.0%
CCCAGATTGTAAAAAGCAAT+TGG + tig0017748:7589-7608 None:intergenic 35.0%
TTTCTGCATGGTAAAACAAG+AGG + tig0017748:7798-7817 None:intergenic 35.0%
CACTACATGAAAGTGACAAA+TGG + tig0017748:8013-8032 None:intergenic 35.0%
!! TTGTGTTGACAATTTTAGGC+AGG - tig0017748:8511-8530 Msa1362250:CDS 35.0%
!! TGTGTTGACAATTTTAGGCA+GGG - tig0017748:8512-8531 Msa1362250:CDS 35.0%
TAAGTTGTTTATTGAGCCCA+TGG + tig0017748:8538-8557 None:intergenic 35.0%
AGTACTCCAATCAAGATAAG+CGG + tig0017748:8601-8620 None:intergenic 35.0%
AAAGAGAATAACGACACATG+GGG + tig0017748:8674-8693 None:intergenic 35.0%
GAAAAGAGAATAACGACACA+TGG + tig0017748:8676-8695 None:intergenic 35.0%
AATTGGTACTGCTTTACCTT+TGG - tig0017748:8794-8813 Msa1362250:intron 35.0%
TTATCCTCGAGAATGAACTT+TGG + tig0017748:9034-9053 None:intergenic 35.0%
ATTTCCCAGTGGATATTGAA+AGG - tig0017748:9056-9075 Msa1362250:CDS 35.0%
CTATCCTTTCAATATCCACT+GGG + tig0017748:9063-9082 None:intergenic 35.0%
ACTATCCTTTCAATATCCAC+TGG + tig0017748:9064-9083 None:intergenic 35.0%
!!! AATTTCTTGTCCGCGTTTTT+TGG - tig0017748:9263-9282 Msa1362250:intron 35.0%
TTGTACTTGTCCAAAAAACG+CGG + tig0017748:9276-9295 None:intergenic 35.0%
ACATAAATCTGACTGAGGTT+TGG - tig0017748:9299-9318 Msa1362250:intron 35.0%
CATAAATCTGACTGAGGTTT+GGG - tig0017748:9300-9319 Msa1362250:intron 35.0%
! TTTTGGTCTGCTACAAAAGT+TGG - tig0017748:9327-9346 Msa1362250:intron 35.0%
TGTATTTGATGTCATGTCAG+AGG + tig0017748:9378-9397 None:intergenic 35.0%
TGATTGTAGTTTCTGACTGT+AGG - tig0017748:9427-9446 Msa1362250:intron 35.0%
!! AGTTTTGTAGTCATGAGTTC+AGG + tig0017748:9690-9709 None:intergenic 35.0%
TATGATGAGAAATTCGTCCT+CGG + tig0017748:9718-9737 None:intergenic 35.0%
! AAAATGCTGTAGATTTTGCG+CGG - tig0017748:9772-9791 Msa1362250:CDS 35.0%
AGACAATTTAGCTGTTGTTG+TGG - tig0017748:9872-9891 Msa1362250:CDS 35.0%
! GAAAGAGTTGCAAAGCTTTT+TGG - tig0017748:9974-9993 Msa1362250:five_prime_UTR 35.0%
! CAAAGCTTTTTGGATAGCTT+GGG - tig0017748:9984-10003 Msa1362250:five_prime_UTR 35.0%
TGAGTTTGCGCAAGTATTTA+TGG - tig0017748:10011-10030 Msa1362250:five_prime_UTR 35.0%
!! AACTGTAGGTGTTACTTGTT+AGG - tig0017748:10120-10139 Msa1362250:five_prime_UTR 35.0%
! TGTTACTTGTTAGGTGTAGT+AGG - tig0017748:10129-10148 Msa1362250:five_prime_UTR 35.0%
CTTTCTGACTGTATCATATG+CGG - tig0017748:10163-10182 Msa1362250:five_prime_UTR 35.0%
AGAATGACCGTCGTAAATGA+AGG + tig0017748:7445-7464 None:intergenic 40.0%
! AAAAGAGTGTGTTGGGAAAG+AGG + tig0017748:7469-7488 None:intergenic 40.0%
GAAGGGTGGTTTAACAAATG+TGG + tig0017748:7516-7535 None:intergenic 40.0%
TTATATGTCGGCGATTCGAA+GGG + tig0017748:7533-7552 None:intergenic 40.0%
TTTATATGTCGGCGATTCGA+AGG + tig0017748:7534-7553 None:intergenic 40.0%
!!! GGGTCTGTGTTTTTGAAGTT+TGG - tig0017748:7637-7656 Msa1362250:CDS 40.0%
GGTGTGAATTGTTTCAGTGA+AGG - tig0017748:7693-7712 Msa1362250:CDS 40.0%
!! ATGGATGGATTAGGATTTGG+AGG + tig0017748:7741-7760 None:intergenic 40.0%
!! GCAATGGATGGATTAGGATT+TGG + tig0017748:7744-7763 None:intergenic 40.0%
TTGACGAAAAGCAGAAGCAA+TGG + tig0017748:7760-7779 None:intergenic 40.0%
! CGAACAAGAGATTTTCTGCA+TGG + tig0017748:7810-7829 None:intergenic 40.0%
!!! GAAATAATGATGGCGGGTTT+TGG - tig0017748:8128-8147 Msa1362250:intron 40.0%
!!! GGTTTTGGTTTGGTCAGATT+GGG - tig0017748:8143-8162 Msa1362250:intron 40.0%
!!! GTTTTGGTTTGGTCAGATTG+GGG - tig0017748:8144-8163 Msa1362250:intron 40.0%
TGGATTCAGTTTGCAACCTT+AGG - tig0017748:8220-8239 Msa1362250:intron 40.0%
! GGTGTTAGTAGGGTTATTTC+TGG - tig0017748:8260-8279 Msa1362250:intron 40.0%
TCTGTTGAGGATGTTACTGA+AGG - tig0017748:8404-8423 Msa1362250:CDS 40.0%
ACACATCCTCCATATTTGAC+CGG + tig0017748:8487-8506 None:intergenic 40.0%
!! TATACCCCATAGAAAGCACT+CGG + tig0017748:8569-8588 None:intergenic 40.0%
GAACTTCCAAAAGATGCCAT+CGG + tig0017748:8737-8756 None:intergenic 40.0%
ACGTTATATGTCGAATGGCT+TGG - tig0017748:8950-8969 Msa1362250:intron 40.0%
TTTGCCAAAGTTCATTCTCG+AGG - tig0017748:9027-9046 Msa1362250:CDS 40.0%
TGCATAGTCTGTTTGCAGAA+AGG + tig0017748:9095-9114 None:intergenic 40.0%
GCAAACAGACTATGCATTTG+CGG - tig0017748:9099-9118 Msa1362250:CDS 40.0%
!! GCATTGGCTACTCTTGTTAT+AGG - tig0017748:9163-9182 Msa1362250:CDS 40.0%
! TGGCTACTCTTGTTATAGGA+AGG - tig0017748:9167-9186 Msa1362250:CDS 40.0%
! ACTCTTGTTATAGGAAGGTC+AGG - tig0017748:9172-9191 Msa1362250:CDS 40.0%
!! ACTGAGGTTTGGGATCTTTT+TGG - tig0017748:9310-9329 Msa1362250:intron 40.0%
! TCAGAGGAAGAGCATGAAAA+TGG + tig0017748:9362-9381 None:intergenic 40.0%
CTTAATGTTGCTCGAGCTAT+TGG - tig0017748:9609-9628 Msa1362250:intron 40.0%
GAAGGAATGAAAGGCAAAGA+TGG - tig0017748:9645-9664 Msa1362250:intron 40.0%
GAGGACGAATTTCTCATCAT+AGG - tig0017748:9717-9736 Msa1362250:intron 40.0%
! GCGCAAAATCTACAGCATTT+TGG + tig0017748:9773-9792 None:intergenic 40.0%
!! GAGGCTTTGAAGAGAAAAAG+TGG - tig0017748:9849-9868 Msa1362250:CDS 40.0%
! GCAAAGCTTTTTGGATAGCT+TGG - tig0017748:9983-10002 Msa1362250:five_prime_UTR 40.0%
ATGACCGTCGTAAATGAAGG+CGG + tig0017748:7442-7461 None:intergenic 45.0%
! GCAGAAGCAATGGATGGATT+AGG + tig0017748:7750-7769 None:intergenic 45.0%
CGAAAAGCAGAAGCAATGGA+TGG + tig0017748:7756-7775 None:intergenic 45.0%
GGTTTCTTACAAGAGAAGCG+TGG + tig0017748:7833-7852 None:intergenic 45.0%
!!! AATGATGGCGGGTTTTGGTT+TGG - tig0017748:8133-8152 Msa1362250:intron 45.0%
!!! GGGTTTTGGTTTGGTCAGAT+TGG - tig0017748:8142-8161 Msa1362250:intron 45.0%
GTTGTCTGATGCATCTGTTG+AGG - tig0017748:8391-8410 Msa1362250:CDS 45.0%
!! GCTTTGGAAGGAAATCAGAC+TGG + tig0017748:8433-8452 None:intergenic 45.0%
TATCGGGTTCCGGTCAAATA+TGG - tig0017748:8475-8494 Msa1362250:CDS 45.0%
! ACAATTTTAGGCAGGGCCAT+GGG - tig0017748:8519-8538 Msa1362250:CDS 45.0%
ATGGACCGAGTGCTTTCTAT+GGG - tig0017748:8561-8580 Msa1362250:CDS 45.0%
CTCTCTCCGCTTATCTTGAT+TGG - tig0017748:8592-8611 Msa1362250:intron 45.0%
GTACTGCTTTACCTTTGGTG+TGG - tig0017748:8799-8818 Msa1362250:intron 45.0%
!! ATATGTCGAATGGCTTGGTC+AGG - tig0017748:8955-8974 Msa1362250:intron 45.0%
CGAGGATAAAGATTTCCCAG+TGG - tig0017748:9045-9064 Msa1362250:CDS 45.0%
AACAAGAGTAGCCAATGCAG+TGG + tig0017748:9161-9180 None:intergenic 45.0%
AGTTTCTGACTGTAGGTTGC+TGG - tig0017748:9434-9453 Msa1362250:intron 45.0%
!! TGTCGATTGCTTTTCCACGA+CGG + tig0017748:9497-9516 None:intergenic 45.0%
ATGTCAAGAGACCACAAACC+AGG - tig0017748:9516-9535 Msa1362250:intron 45.0%
! CTTTCATGCAACCTGGTTTG+TGG + tig0017748:9530-9549 None:intergenic 45.0%
TGGCACATGGAAGGAATGAA+AGG - tig0017748:9636-9655 Msa1362250:intron 45.0%
GGAATGAAAGGCAAAGATGG+TGG - tig0017748:9648-9667 Msa1362250:intron 45.0%
GACTACAAAACTTACTGCCG+AGG - tig0017748:9698-9717 Msa1362250:intron 45.0%
TTTCTCATCATAGGCTGCGA+TGG - tig0017748:9726-9745 Msa1362250:intron 45.0%
TTCTCATCATAGGCTGCGAT+GGG - tig0017748:9727-9746 Msa1362250:intron 45.0%
GATCCTTACTGCATACAGCT+GGG + tig0017748:9821-9840 None:intergenic 45.0%
AGATCCTTACTGCATACAGC+TGG + tig0017748:9822-9841 None:intergenic 45.0%
AGCTGTATGCAGTAAGGATC+TGG - tig0017748:9821-9840 Msa1362250:CDS 45.0%
! CAGTAAGGATCTGGTTGATG+AGG - tig0017748:9830-9849 Msa1362250:CDS 45.0%
! TTTGGGTTTCGAAGATGCGG+TGG + tig0017748:7399-7418 None:intergenic 50.0%
! GGGTTTGGGTTTCGAAGATG+CGG + tig0017748:7402-7421 None:intergenic 50.0%
TCGTAAATGAAGGCGGTGGT+CGG + tig0017748:7435-7454 None:intergenic 50.0%
ACCACCGCCTTCATTTACGA+CGG - tig0017748:7435-7454 Msa1362250:intron 50.0%
TTCCTTCCAAAGCTCCGTTC+CGG - tig0017748:8440-8459 Msa1362250:CDS 50.0%
TATCAGCACATCCTCCGGAA+CGG + tig0017748:8457-8476 None:intergenic 50.0%
! TCCGGAGGATGTGCTGATAT+CGG - tig0017748:8458-8477 Msa1362250:CDS 50.0%
! GATGTGCTGATATCGGGTTC+CGG - tig0017748:8465-8484 Msa1362250:CDS 50.0%
! GACAATTTTAGGCAGGGCCA+TGG - tig0017748:8518-8537 Msa1362250:CDS 50.0%
GATGGACCGAGTGCTTTCTA+TGG - tig0017748:8560-8579 Msa1362250:CDS 50.0%
TGGACCGAGTGCTTTCTATG+GGG - tig0017748:8562-8581 Msa1362250:CDS 50.0%
! GCACATCCGATGGCATCTTT+TGG - tig0017748:8728-8747 Msa1362250:intron 50.0%
TGCACATTGGTCCACACCAA+AGG + tig0017748:8813-8832 None:intergenic 50.0%
AGACGCGCCTTTACTAGCTA+TGG - tig0017748:8845-8864 Msa1362250:intron 50.0%
!! TGGCTTGGTCAGGTGTTTGA+TGG - tig0017748:8965-8984 Msa1362250:intron 50.0%
! GGTCAGGTGTTTGATGGACA+CGG - tig0017748:8971-8990 Msa1362250:intron 50.0%
TTCTGACTGTAGGTTGCTGG+TGG - tig0017748:9437-9456 Msa1362250:intron 50.0%
CTCTGCTCTTTCATGCAACC+TGG + tig0017748:9537-9556 None:intergenic 50.0%
CAGGTTGCATGAAAGAGCAG+AGG - tig0017748:9535-9554 Msa1362250:intron 50.0%
! TTGCTCGAGCTATTGGTGAC+TGG - tig0017748:9616-9635 Msa1362250:intron 50.0%
! AGCTATTGGTGACTGGCACA+TGG - tig0017748:9623-9642 Msa1362250:intron 50.0%
! ATTGGTGACTGGCACATGGA+AGG - tig0017748:9627-9646 Msa1362250:intron 50.0%
TCATAGGCTGCGATGGGATT+TGG - tig0017748:9733-9752 Msa1362250:CDS 50.0%
CATAGGCTGCGATGGGATTT+GGG - tig0017748:9734-9753 Msa1362250:CDS 50.0%
! GGTGCTACCAAATTTGGTGG+AGG + tig0017748:9915-9934 None:intergenic 50.0%
!! CGTGGTGCTACCAAATTTGG+TGG + tig0017748:9918-9937 None:intergenic 50.0%
!! GAGCGTGGTGCTACCAAATT+TGG + tig0017748:9921-9940 None:intergenic 50.0%
TAAATGAAGGCGGTGGTCGG+TGG + tig0017748:7432-7451 None:intergenic 55.0%
ACCGTCGTAAATGAAGGCGG+TGG + tig0017748:7439-7458 None:intergenic 55.0%
TATGTCGGCGATTCGAAGGG+TGG + tig0017748:7530-7549 None:intergenic 55.0%
CCCGATATCAGCACATCCTC+CGG + tig0017748:8462-8481 None:intergenic 55.0%
! CCGGAGGATGTGCTGATATC+GGG - tig0017748:8459-8478 Msa1362250:CDS 55.0%
CGGGTTCCGGTCAAATATGG+AGG - tig0017748:8478-8497 Msa1362250:CDS 55.0%
CTCCTGTTCTGCACATCCGA+TGG - tig0017748:8718-8737 Msa1362250:intron 55.0%
TGCCATCGGATGTGCAGAAC+AGG + tig0017748:8723-8742 None:intergenic 55.0%
! CAGGTGTTTGATGGACACGG+CGG - tig0017748:8974-8993 Msa1362250:intron 55.0%
CTCAATGCTGCCCTTGCATC+CGG - tig0017748:9133-9152 Msa1362250:CDS 55.0%
AGTAGCCAATGCAGTGGTGC+CGG + tig0017748:9155-9174 None:intergenic 55.0%
TGACTGTAGGTTGCTGGTGG+TGG - tig0017748:9440-9459 Msa1362250:intron 55.0%
!! TTGCTGGTGGTGGCAAATGC+TGG - tig0017748:9450-9469 Msa1362250:intron 55.0%
! GAAAGAGCAGAGGCGTATCG+AGG - tig0017748:9545-9564 Msa1362250:intron 55.0%
! AGAGGCGTATCGAGGCATCT+GGG - tig0017748:9553-9572 Msa1362250:intron 55.0%
TCTGGGGGGTCTGTCTATGA+TGG - tig0017748:9570-9589 Msa1362250:intron 55.0%
AGTTCAGGCTCTGCACTGAG+TGG + tig0017748:9675-9694 None:intergenic 55.0%
CGACCCAGCTGTATGCAGTA+AGG - tig0017748:9815-9834 Msa1362250:CDS 55.0%
GCAGCAGCCTCCACCAAATT+TGG - tig0017748:9905-9924 Msa1362250:five_prime_UTR 55.0%
AGAGAAGCTCCTGTGCACTC+TGG + tig0017748:9943-9962 None:intergenic 55.0%
CACAGGAGCTTCTCTGCTGA+AGG - tig0017748:9948-9967 Msa1362250:five_prime_UTR 55.0%
TCGGTGGTCGGTGAGGGTTT+GGG + tig0017748:7416-7435 None:intergenic 60.0%
!! CTCCGGAACGGAGCTTTGGA+AGG + tig0017748:8445-8464 None:intergenic 60.0%
CTTCCAAAGCTCCGTTCCGG+AGG - tig0017748:8443-8462 Msa1362250:CDS 60.0%
CATCCTCCGGAACGGAGCTT+TGG + tig0017748:8449-8468 None:intergenic 60.0%
! TGCAGTGGTGCCGGATGCAA+GGG + tig0017748:9146-9165 None:intergenic 60.0%
! ATGCAGTGGTGCCGGATGCA+AGG + tig0017748:9147-9166 None:intergenic 60.0%
TGCATCCGGCACCACTGCAT+TGG - tig0017748:9147-9166 Msa1362250:CDS 60.0%
! CAGAGGCGTATCGAGGCATC+TGG - tig0017748:9552-9571 Msa1362250:intron 60.0%
! GAGGCGTATCGAGGCATCTG+GGG - tig0017748:9554-9573 Msa1362250:intron 60.0%
! AGGCGTATCGAGGCATCTGG+GGG - tig0017748:9555-9574 Msa1362250:intron 60.0%
! CTGTAGATTTTGCGCGGCGC+AGG - tig0017748:9778-9797 Msa1362250:CDS 60.0%
GTCGGTGGTCGGTGAGGGTT+TGG + tig0017748:7417-7436 None:intergenic 65.0%
TGAAGGCGGTGGTCGGTGGT+CGG + tig0017748:7428-7447 None:intergenic 65.0%
! GGCGTATCGAGGCATCTGGG+GGG - tig0017748:9556-9575 Msa1362250:intron 65.0%
! TTTTGCGCGGCGCAGGCTTC+AGG - tig0017748:9785-9804 Msa1362250:CDS 65.0%
CACCACGCTCCAGAGTGCAC+AGG - tig0017748:9931-9950 Msa1362250:five_prime_UTR 65.0%
!! CTCCTGTGCACTCTGGAGCG+TGG + tig0017748:9936-9955 None:intergenic 65.0%
CGGTGGTCGGTGGTCGGTGA+GGG + tig0017748:7422-7441 None:intergenic 70.0%
CGAGCAGTGCTGTGCCGTCG+TGG - tig0017748:9480-9499 Msa1362250:intron 70.0%
GCGGTGGTCGGTGGTCGGTG+AGG + tig0017748:7423-7442 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
tig0017748 gene 7335 10235 7335 ID=Msa1362250;Name=Msa1362250
tig0017748 mRNA 7335 10235 7335 ID=Msa1362250-mRNA-1;Parent=Msa1362250;Name=Msa1362250-mRNA-1;_AED=0.12;_eAED=0.12;_QI=343|1|1|1|0.75|0.6|5|193|382
tig0017748 exon 7335 7442 7335 ID=Msa1362250-mRNA-1:exon:12932;Parent=Msa1362250-mRNA-1
tig0017748 exon 7472 8121 7472 ID=Msa1362250-mRNA-1:exon:12931;Parent=Msa1362250-mRNA-1
tig0017748 exon 8382 8593 8382 ID=Msa1362250-mRNA-1:exon:12930;Parent=Msa1362250-mRNA-1
tig0017748 exon 8987 9193 8987 ID=Msa1362250-mRNA-1:exon:12929;Parent=Msa1362250-mRNA-1
tig0017748 exon 9728 10235 9728 ID=Msa1362250-mRNA-1:exon:12928;Parent=Msa1362250-mRNA-1
tig0017748 five_prime_UTR 9893 10235 9893 ID=Msa1362250-mRNA-1:five_prime_utr;Parent=Msa1362250-mRNA-1
tig0017748 CDS 9728 9892 9728 ID=Msa1362250-mRNA-1:cds;Parent=Msa1362250-mRNA-1
tig0017748 CDS 8987 9193 8987 ID=Msa1362250-mRNA-1:cds;Parent=Msa1362250-mRNA-1
tig0017748 CDS 8382 8593 8382 ID=Msa1362250-mRNA-1:cds;Parent=Msa1362250-mRNA-1
tig0017748 CDS 7557 8121 7557 ID=Msa1362250-mRNA-1:cds;Parent=Msa1362250-mRNA-1
tig0017748 three_prime_UTR 7472 7556 7472 ID=Msa1362250-mRNA-1:three_prime_utr;Parent=Msa1362250-mRNA-1
tig0017748 three_prime_UTR 7335 7442 7335 ID=Msa1362250-mRNA-1:three_prime_utr;Parent=Msa1362250-mRNA-1
Gene Sequence

>Msa1362250

ATGGAAGAAATTGAAGGTGTGAATTGTTTCAGTGAAGGTAGAAGCTCTAGTGAGAGTCGACCTCCAAATCCTAATCCATCCATTGCTTCTGCTTTTCGTCAATGTCAACGCAATGATCCTCTTGTTTTACCATGCAGAAAATCTCTTGTTCGCCACGCTTCTCTTCAGACGAAAATGAAGTTGTCTGATGCATCTGTTGAGGATGTTACTGAAGGTTGCCAGTCTGATTTCCTTCCAAAGCTCCGTTCCGGAGGATGTGCTGATATCGGGTTCCGGTCAAATATGGAGGATGTGTATGTTTGTGTTGACAATTTTAGGCAGGGCCATGGGCTCAATAAACAACTTATAGATGGACCGAGTGCTTTCTATGGGGTGTTTGATGGACACGGCGGAAAGCATGCTGCTGACTTTGCTTGCAATCATTTGCCAAAGTTCATTCTCGAGGATAAAGATTTCCCAGTGGATATTGAAAGGATAGTTGCTTCTGCCTTTCTGCAAACAGACTATGCATTTGCGGAAGCTTGTTCTCTCAATGCTGCCCTTGCATCCGGCACCACTGCATTGGCTACTCTTGTTATAGGAAGGTTGCTGGTGGTGGCAAATGCTGGAGATTGTCGAGCAGTGCTGTGCCGTCGTGGAAAAGCAATCGACATGTCAAGAGACCACAAACCAGGTTGCATGAAAGAGCAGAGGCGTATCGAGGCATCTGGGGGGTCTGTCTATGATGGATATCTTAACGGGCAACTTAATGTTGCTCGAGCTATTGGTGACTGGCACATGGAAGGAATGAAAGGCAAAGATGGTGGACCACTCAGTGCAGAGCCTGAACTCATGACTACAAAACTTACTGCCGAGGACGAATTTCTCATCATAGGCTGCGATGGGATTTGGGATGTTTTTAGAAGCCAAAATGCTGTAGATTTTGCGCGGCGCAGGCTTCAGGTGCATAACGACCCAGCTGTATGCAGTAAGGATCTGGTTGATGAGGCTTTGAAGAGAAAAAGTGGAGACAATTTAGCTGTTGTTGTGGTTTGCTTTCAGCAGCAGCCTCCACCAAATTTGGTAGCACCACGCTCCAGAGTGCACAGGAGCTTCTCTGCTGAAGGTTTGAAAGAGTTGCAAAGCTTTTTGGATAGCTTGGGAAAATGA
Protein sequence

>Msa1362250

MEEIEGVNCFSEGRSSSESRPPNPNPSIASAFRQCQRNDPLVLPCRKSLVRHASLQTKMKLSDASVEDVTEGCQSDFLPKLRSGGCADIGFRSNMEDVYVCVDNFRQGHGLNKQLIDGPSAFYGVFDGHGGKHAADFACNHLPKFILEDKDFPVDIERIVASAFLQTDYAFAEACSLNAALASGTTALATLVIGRLLVVANAGDCRAVLCRRGKAIDMSRDHKPGCMKEQRRIEASGGSVYDGYLNGQLNVARAIGDWHMEGMKGKDGGPLSAEPELMTTKLTAEDEFLIIGCDGIWDVFRSQNAVDFARRRLQVHNDPAVCSKDLVDEALKRKSGDNLAVVVVCFQQQPPPNLVAPRSRVHRSFSAEGLKELQSFLDSLGK*