AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1415580

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1415580 MtrunA17_Chr3g0138281 97.644 382 8 1 1 382 1 381 0.0 766
Msa1415580 MtrunA17_Chr4g0065141 58.497 306 123 3 76 378 79 383 4.47e-127 370
Msa1415580 MtrunA17_Chr5g0407161 54.575 306 133 4 74 378 77 377 1.66e-112 333
Msa1415580 MtrunA17_Chr3g0144111 54.577 284 104 4 95 378 1 259 1.08e-99 296
Msa1415580 MtrunA17_Chr1g0194191 45.402 348 160 8 49 378 50 385 1.37e-89 275
Msa1415580 MtrunA17_Chr2g0293811 43.491 338 174 7 55 379 34 367 2.91e-85 263
Msa1415580 MtrunA17_Chr1g0150451 36.162 271 147 8 80 346 75 323 1.80e-40 146
Msa1415580 MtrunA17_Chr1g0150071 29.714 350 177 8 61 357 5 338 2.85e-40 146
Msa1415580 MtrunA17_Chr1g0171571 35.106 282 148 9 77 344 382 642 3.08e-40 150
Msa1415580 MtrunA17_Chr3g0136811 29.944 354 175 9 59 357 3 338 7.12e-40 145
Msa1415580 MtrunA17_Chr3g0136711 35.055 271 150 8 80 346 32 280 1.24e-38 140
Msa1415580 MtrunA17_Chr4g0001761 30.530 321 156 7 81 347 22 329 1.45e-38 141
Msa1415580 MtrunA17_Chr5g0430481 35.926 270 138 7 90 348 131 376 1.76e-38 141
Msa1415580 MtrunA17_Chr6g0483411 31.392 309 170 8 66 348 28 320 1.21e-37 138
Msa1415580 MtrunA17_Chr3g0105371 36.090 266 141 7 90 347 95 339 1.22e-37 138
Msa1415580 MtrunA17_Chr4g0068971 45.679 162 76 4 224 378 110 266 1.71e-37 136
Msa1415580 MtrunA17_Chr3g0105351 35.821 268 139 8 90 347 71 315 3.85e-37 136
Msa1415580 MtrunA17_Chr1g0212961 35.227 264 143 8 90 350 103 341 8.18e-37 136
Msa1415580 MtrunA17_Chr3g0115851 35.983 239 134 5 114 347 58 282 2.90e-36 133
Msa1415580 MtrunA17_Chr5g0426141 35.271 258 139 8 95 347 48 282 1.60e-35 131
Msa1415580 MtrunA17_Chr3g0126511 32.632 285 165 7 69 349 82 343 2.81e-35 132
Msa1415580 MtrunA17_Chr2g0279151 36.797 231 127 6 122 347 65 281 1.41e-34 129
Msa1415580 MtrunA17_Chr4g0050381 35.065 231 131 5 122 347 61 277 2.17e-32 123
Msa1415580 MtrunA17_Chr2g0317021 34.167 240 139 6 113 347 108 333 3.28e-31 121
Msa1415580 MtrunA17_Chr2g0299981 29.467 319 160 9 89 349 227 538 7.44e-30 120
Msa1415580 MtrunA17_Chr3g0127091 27.152 302 171 7 79 343 57 346 8.57e-29 115
Msa1415580 MtrunA17_Chr1g0150781 29.870 308 160 10 90 349 247 546 9.10e-29 117
Msa1415580 MtrunA17_Chr8g0360351 33.195 241 140 7 113 347 57 282 5.45e-28 111
Msa1415580 MtrunA17_Chr4g0070871 29.836 305 159 9 84 344 208 501 8.63e-28 114
Msa1415580 MtrunA17_Chr3g0112291 32.184 261 135 7 119 344 120 373 9.17e-28 112
Msa1415580 MtrunA17_Chr4g0051661 33.824 272 147 10 83 346 151 397 5.55e-27 110
Msa1415580 MtrunA17_Chr1g0158781 33.333 285 151 9 81 344 111 377 2.06e-26 108
Msa1415580 MtrunA17_Chr3g0135961 29.710 276 151 7 110 349 274 542 2.65e-26 110
Msa1415580 MtrunA17_Chr5g0396731 33.981 206 118 4 122 316 107 305 2.32e-25 105
Msa1415580 MtrunA17_Chr8g0389921 28.617 311 174 8 84 363 150 443 6.40e-25 105
Msa1415580 MtrunA17_Chr5g0435721 34.112 214 112 6 122 316 223 426 2.33e-24 104
Msa1415580 MtrunA17_Chr7g0263281 27.778 288 147 12 118 349 66 348 2.22e-18 85.9
Msa1415580 MtrunA17_Chr2g0295401 28.938 273 139 13 124 350 94 357 2.58e-18 85.5
Msa1415580 MtrunA17_Chr1g0211251 27.915 283 143 12 118 344 76 353 6.46e-18 84.7
Msa1415580 MtrunA17_Chr4g0066371 29.434 265 133 11 122 344 86 338 1.01e-17 84.0
Msa1415580 MtrunA17_Chr4g0067711 27.097 310 169 13 92 350 53 356 1.02e-17 83.6
Msa1415580 MtrunA17_Chr3g0089721 29.075 227 121 6 122 311 68 291 1.07e-17 83.6
Msa1415580 MtrunA17_Chr3g0089711 28.384 229 124 6 120 311 66 291 2.66e-17 82.4
Msa1415580 MtrunA17_Chr7g0256751 27.490 251 141 8 122 337 69 313 4.16e-17 82.4
Msa1415580 MtrunA17_Chr8g0371321 26.333 300 156 13 118 361 74 364 5.70e-17 81.6
Msa1415580 MtrunA17_Chr1g0151781 30.045 223 109 8 121 303 67 282 5.82e-17 81.3
Msa1415580 MtrunA17_Chr7g0250061 26.351 296 154 12 121 361 75 361 6.84e-17 81.6
Msa1415580 MtrunA17_Chr1g0213351 26.481 287 149 12 121 351 79 359 1.05e-16 80.9
Msa1415580 MtrunA17_Chr3g0133801 27.881 269 152 9 117 360 59 310 3.09e-16 79.7
Msa1415580 MtrunA17_Chr1g0192421 28.400 250 129 10 122 331 89 328 6.93e-16 78.2
Msa1415580 MtrunA17_Chr1g0181901 28.395 243 121 11 118 319 71 301 4.30e-15 76.3
Msa1415580 MtrunA17_Chr2g0294291 28.627 255 141 10 114 332 66 315 8.44e-15 75.1
Msa1415580 MtrunA17_Chr5g0394181 29.218 243 147 10 122 346 362 597 1.06e-14 75.5
Msa1415580 MtrunA17_Chr7g0272061 28.195 266 127 11 122 334 91 345 3.83e-14 73.2
Msa1415580 MtrunA17_Chr7g0249421 35.417 144 69 4 183 308 206 343 4.46e-14 73.6
Msa1415580 MtrunA17_Chr7g0256771 28.634 227 120 7 122 310 70 292 5.22e-14 72.4
Msa1415580 MtrunA17_Chr7g0243571 27.208 283 146 10 122 350 92 368 1.35e-13 72.0
Msa1415580 MtrunA17_Chr7g0238501 26.667 225 126 7 121 306 262 486 3.39e-13 70.9
Msa1415580 MtrunA17_Chr7g0221691 35.172 145 70 5 183 309 231 369 8.50e-13 69.3
Msa1415580 MtrunA17_Chr1g0209451 29.278 263 137 13 121 357 67 306 2.38e-12 68.2
Msa1415580 MtrunA17_Chr7g0225501 30.698 215 108 12 117 303 79 280 3.87e-11 64.3
Msa1415580 MtrunA17_Chr4g0004971 26.991 226 113 8 120 300 76 294 4.49e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1415580 AT2G25620.1 66.244 394 117 7 1 381 1 391 0.0 510
Msa1415580 AT2G25620.2 66.244 394 117 7 1 381 1 391 0.0 510
Msa1415580 AT2G33700.1 55.556 306 131 4 74 378 76 377 2.05e-114 338
Msa1415580 AT3G51470.1 52.581 310 138 3 71 380 61 361 1.15e-109 326
Msa1415580 AT3G51470.2 52.581 310 138 3 71 380 17 317 1.19e-109 324
Msa1415580 AT3G62260.1 48.734 316 145 6 76 378 71 382 5.52e-90 276
Msa1415580 AT3G62260.2 48.734 316 145 6 76 378 72 383 6.55e-90 276
Msa1415580 AT1G48040.1 46.006 313 156 5 75 377 71 380 1.41e-83 259
Msa1415580 AT3G62260.3 48.485 297 136 6 95 378 1 293 1.93e-82 254
Msa1415580 AT3G17250.1 42.902 317 163 6 75 378 110 421 1.08e-74 238
Msa1415580 AT5G24940.1 36.531 271 146 8 80 346 31 279 3.23e-42 153
Msa1415580 AT5G10740.1 37.269 271 144 8 80 346 31 279 6.36e-42 150
Msa1415580 AT5G10740.2 37.269 271 144 8 80 346 31 279 6.36e-42 150
Msa1415580 AT4G31750.1 36.531 271 146 8 80 346 31 279 6.22e-40 144
Msa1415580 AT4G31750.3 36.531 271 146 8 80 346 31 279 6.22e-40 144
Msa1415580 AT4G31750.2 36.531 271 146 8 80 346 31 279 6.65e-40 144
Msa1415580 AT2G25070.1 28.895 353 179 9 61 358 5 340 6.75e-40 145
Msa1415580 AT2G25070.2 28.895 353 179 9 61 358 5 340 6.75e-40 145
Msa1415580 AT5G53140.1 33.456 272 155 7 81 348 100 349 1.20e-39 146
Msa1415580 AT5G53140.2 33.456 272 155 7 81 348 100 349 1.20e-39 146
Msa1415580 AT4G31860.1 29.969 327 161 7 80 351 21 334 5.16e-39 143
Msa1415580 AT1G78200.5 35.498 231 133 3 122 347 19 238 1.50e-37 135
Msa1415580 AT1G78200.4 35.498 231 133 3 122 347 19 238 1.50e-37 135
Msa1415580 AT2G20630.1 33.721 258 143 6 95 347 45 279 2.08e-37 136
Msa1415580 AT1G78200.1 35.498 231 133 3 122 347 64 283 3.66e-37 136
Msa1415580 AT1G78200.3 35.498 231 133 3 122 347 64 283 3.66e-37 136
Msa1415580 AT1G78200.2 35.498 231 133 3 122 347 64 283 3.66e-37 136
Msa1415580 AT2G20630.2 33.463 257 143 6 95 346 45 278 3.98e-37 136
Msa1415580 AT2G30020.1 36.431 269 140 9 90 348 147 394 8.35e-37 138
Msa1415580 AT4G28400.3 34.496 258 141 6 95 347 27 261 9.60e-37 134
Msa1415580 AT4G28400.1 34.496 258 141 6 95 347 49 283 1.92e-36 134
Msa1415580 AT4G31860.3 31.690 284 131 6 80 313 21 291 6.22e-36 133
Msa1415580 AT2G40860.4 32.404 287 158 9 75 347 287 551 7.70e-36 138
Msa1415580 AT2G40860.1 32.746 284 155 9 75 344 385 646 9.85e-36 138
Msa1415580 AT1G43900.1 34.066 273 151 9 80 347 121 369 2.20e-35 133
Msa1415580 AT1G18030.1 31.944 288 152 9 88 348 80 350 3.27e-35 132
Msa1415580 AT1G07160.1 35.338 266 140 8 90 344 130 374 9.61e-35 132
Msa1415580 AT1G22280.1 35.271 258 139 9 95 347 47 281 1.18e-33 127
Msa1415580 AT3G15260.2 34.855 241 136 6 113 347 64 289 1.20e-33 127
Msa1415580 AT3G15260.1 34.855 241 136 6 113 347 64 289 1.20e-33 127
Msa1415580 AT1G34750.2 34.468 235 134 6 116 344 59 279 6.70e-33 124
Msa1415580 AT1G34750.1 34.468 235 134 6 116 344 59 279 6.70e-33 124
Msa1415580 AT1G34750.3 34.468 235 134 6 116 344 59 279 6.70e-33 124
Msa1415580 AT1G67820.2 38.342 193 100 4 121 306 114 294 3.41e-32 125
Msa1415580 AT1G67820.1 38.342 193 100 4 121 306 150 330 4.14e-32 126
Msa1415580 AT2G40180.1 34.182 275 148 8 90 351 136 390 4.25e-32 125
Msa1415580 AT4G31860.2 30.970 268 122 6 80 297 21 275 2.05e-31 120
Msa1415580 AT1G22280.3 34.470 264 139 10 95 347 47 287 3.41e-31 120
Msa1415580 AT2G40860.2 31.387 274 152 9 75 334 385 636 5.46e-31 124
Msa1415580 AT2G29380.1 32.226 301 147 12 82 344 77 358 1.17e-28 115
Msa1415580 AT1G07430.1 31.890 254 144 8 74 310 114 355 1.40e-28 116
Msa1415580 AT4G26080.1 30.201 298 152 10 90 344 136 420 3.93e-28 114
Msa1415580 AT2G34740.1 31.801 261 146 9 95 348 101 336 6.32e-28 112
Msa1415580 AT5G51760.2 30.392 306 152 10 89 344 85 379 9.80e-28 113
Msa1415580 AT5G51760.1 30.392 306 152 10 89 344 115 409 1.36e-27 113
Msa1415580 AT5G59220.1 35.000 240 122 8 90 310 119 343 4.32e-27 111
Msa1415580 AT1G18030.2 31.933 238 119 8 88 299 80 300 4.43e-27 109
Msa1415580 AT1G72770.4 29.841 315 159 9 90 349 197 504 2.33e-26 110
Msa1415580 AT1G72770.1 29.841 315 159 9 90 349 197 504 2.33e-26 110
Msa1415580 AT1G72770.3 29.841 315 159 9 90 349 197 504 2.33e-26 110
Msa1415580 AT1G17550.1 28.615 325 163 8 84 349 190 504 2.88e-26 110
Msa1415580 AT1G17550.2 28.615 325 163 8 84 349 190 504 2.88e-26 110
Msa1415580 AT4G27800.1 27.019 322 178 8 81 361 58 363 3.09e-26 108
Msa1415580 AT3G11410.1 32.946 258 132 9 78 310 102 343 3.18e-26 108
Msa1415580 AT5G57050.1 29.373 303 153 11 90 344 120 409 3.75e-26 109
Msa1415580 AT5G57050.2 29.373 303 153 11 90 344 80 369 3.79e-26 108
Msa1415580 AT4G28400.2 34.286 210 114 5 95 299 49 239 6.39e-26 105
Msa1415580 AT1G34750.4 33.158 190 111 5 116 299 59 238 2.83e-22 95.1
Msa1415580 AT2G40860.3 31.897 232 121 9 75 290 385 595 1.32e-21 97.1
Msa1415580 AT2G34740.3 32.075 212 116 8 95 299 97 287 2.13e-21 94.0
Msa1415580 AT2G34740.2 32.075 212 116 8 95 299 115 305 2.27e-21 94.4
Msa1415580 AT4G08260.1 30.859 256 129 10 95 348 1 210 2.67e-21 91.7
Msa1415580 AT4G27800.2 26.531 294 159 8 81 333 58 335 2.19e-20 91.7
Msa1415580 AT4G27800.3 27.465 284 148 9 81 322 58 325 7.89e-20 89.7
Msa1415580 AT5G02760.1 31.780 236 113 11 121 319 71 295 1.54e-19 89.7
Msa1415580 AT5G59220.2 37.097 186 90 7 90 256 119 296 1.04e-18 86.3
Msa1415580 AT3G12620.2 28.975 283 140 13 118 344 78 355 2.76e-18 85.9
Msa1415580 AT3G12620.1 28.975 283 140 13 118 344 78 355 2.76e-18 85.9
Msa1415580 AT2G20050.2 28.625 269 130 9 122 344 143 395 2.78e-17 84.3
Msa1415580 AT2G20050.1 28.625 269 130 9 122 344 143 395 2.83e-17 84.3
Msa1415580 AT3G63340.8 29.496 278 122 12 149 366 198 461 8.72e-17 82.8
Msa1415580 AT3G63340.5 29.496 278 122 12 149 366 198 461 8.72e-17 82.8
Msa1415580 AT3G63340.1 29.496 278 122 12 149 366 198 461 8.72e-17 82.8
Msa1415580 AT3G63340.7 29.496 278 122 12 149 366 198 461 8.80e-17 82.8
Msa1415580 AT3G63340.6 29.496 278 122 12 149 366 198 461 9.69e-17 82.8
Msa1415580 AT3G63340.3 29.496 278 122 12 149 366 198 461 9.69e-17 82.8
Msa1415580 AT3G63340.10 29.496 278 122 12 149 366 198 461 9.69e-17 82.8
Msa1415580 AT3G63340.4 29.496 278 122 12 149 366 198 461 9.69e-17 82.8
Msa1415580 AT3G63340.11 29.496 278 122 12 149 366 198 461 1.04e-16 82.4
Msa1415580 AT5G01700.1 28.315 279 143 11 120 352 27 294 1.15e-16 80.9
Msa1415580 AT3G63340.12 29.496 278 122 12 149 366 198 461 1.33e-16 81.6
Msa1415580 AT4G33920.1 26.027 292 156 12 114 348 52 340 1.97e-16 80.5
Msa1415580 AT1G47380.1 29.104 268 144 10 117 357 57 305 2.42e-16 80.5
Msa1415580 AT5G01700.2 28.315 279 143 11 120 352 76 343 3.59e-16 79.7
Msa1415580 AT3G55050.1 27.273 286 147 13 118 347 79 359 6.17e-16 79.0
Msa1415580 AT3G55050.2 27.273 286 147 13 118 347 79 359 6.17e-16 79.0
Msa1415580 AT3G16800.2 31.556 225 112 9 121 310 92 309 6.91e-16 78.6
Msa1415580 AT3G16800.1 31.556 225 112 9 121 310 92 309 6.91e-16 78.6
Msa1415580 AT3G16800.3 31.556 225 112 9 121 310 92 309 6.91e-16 78.6
Msa1415580 AT3G16800.4 37.415 147 67 5 183 310 74 214 7.88e-16 77.0
Msa1415580 AT3G16800.6 37.415 147 67 5 183 310 74 214 7.88e-16 77.0
Msa1415580 AT3G05640.2 26.756 299 154 13 81 332 48 328 1.99e-15 77.4
Msa1415580 AT3G05640.1 26.756 299 154 13 81 332 48 328 1.99e-15 77.4
Msa1415580 AT3G05640.3 26.756 299 154 13 81 332 48 328 1.99e-15 77.4
Msa1415580 AT3G51370.1 25.088 283 151 11 118 344 74 351 2.31e-15 77.4
Msa1415580 AT3G51370.3 25.088 283 151 11 118 344 74 351 2.31e-15 77.4
Msa1415580 AT3G17090.1 29.032 248 131 9 121 331 83 322 3.32e-15 77.0
Msa1415580 AT3G17090.2 28.926 242 127 9 121 325 83 316 4.15e-15 75.9
Msa1415580 AT4G38520.2 25.795 283 153 11 118 346 76 355 4.30e-15 76.6
Msa1415580 AT4G38520.1 25.795 283 153 11 118 346 76 355 4.30e-15 76.6
Msa1415580 AT4G32950.1 28.641 206 118 6 121 303 71 270 8.21e-15 75.1
Msa1415580 AT3G63320.2 28.571 273 119 12 151 357 52 314 1.35e-14 75.1
Msa1415580 AT1G09160.2 30.315 254 132 11 117 344 61 295 1.53e-14 75.1
Msa1415580 AT1G09160.1 30.315 254 132 11 117 344 61 295 1.53e-14 75.1
Msa1415580 AT5G27930.1 27.600 250 115 11 107 308 82 313 2.39e-14 74.3
Msa1415580 AT5G27930.2 27.600 250 115 11 107 308 82 313 2.39e-14 74.3
Msa1415580 AT5G57050.3 30.256 195 104 8 90 258 120 308 2.97e-14 73.2
Msa1415580 AT3G63320.1 26.706 337 134 14 124 357 71 397 3.51e-14 73.9
Msa1415580 AT1G72770.2 31.220 205 103 6 90 256 197 401 5.43e-14 73.2
Msa1415580 AT1G72770.5 31.220 205 103 6 90 256 197 401 5.43e-14 73.2
Msa1415580 AT1G22280.2 35.616 146 82 5 95 240 47 180 1.18e-13 69.7
Msa1415580 AT5G66080.1 26.855 283 146 11 118 344 77 354 1.36e-13 72.0
Msa1415580 AT5G26010.1 26.493 268 148 10 124 353 75 331 2.64e-13 70.9
Msa1415580 AT3G63340.2 33.918 171 84 8 212 366 337 494 4.76e-13 71.2
Msa1415580 AT1G79630.6 32.275 189 91 8 183 346 111 287 7.78e-13 69.7
Msa1415580 AT1G79630.3 32.275 189 91 8 183 346 111 287 7.78e-13 69.7
Msa1415580 AT1G79630.4 32.275 189 91 8 183 346 111 287 7.78e-13 69.7
Msa1415580 AT1G79630.5 32.275 189 91 8 183 346 111 287 7.78e-13 69.7
Msa1415580 AT1G79630.2 32.292 192 93 8 183 349 151 330 9.25e-13 69.7
Msa1415580 AT1G79630.7 32.292 192 93 8 183 349 151 330 9.25e-13 69.7
Msa1415580 AT1G16220.1 29.515 227 119 10 148 346 161 374 1.26e-12 69.3
Msa1415580 AT1G79630.8 32.275 189 91 8 183 346 217 393 1.31e-12 69.3
Msa1415580 AT1G79630.1 32.275 189 91 8 183 346 221 397 1.38e-12 69.3
Msa1415580 AT5G06750.3 25.357 280 152 10 120 344 80 357 1.89e-12 68.6
Msa1415580 AT5G06750.1 25.357 280 152 10 120 344 80 357 1.89e-12 68.6
Msa1415580 AT5G19280.1 27.419 248 147 10 123 346 339 577 3.26e-12 68.6
Msa1415580 AT3G02750.2 34.507 142 69 5 183 306 215 350 5.05e-12 67.8
Msa1415580 AT3G02750.1 34.507 142 69 5 183 306 215 350 5.05e-12 67.8
Msa1415580 AT4G32950.2 28.141 199 114 6 121 296 71 263 1.04e-11 65.5
Msa1415580 AT3G16560.1 29.054 148 83 4 181 306 273 420 2.67e-11 65.5
Msa1415580 AT3G16560.4 29.054 148 83 4 181 306 273 420 2.67e-11 65.5
Msa1415580 AT3G16560.2 29.054 148 83 4 181 306 273 420 2.67e-11 65.5
Msa1415580 AT3G16560.3 29.054 148 83 4 181 306 273 420 2.68e-11 65.5

Find 127 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGTTACCAAAATTCGAAAA+TGG 0.203487 tig0024251:+15564 None:intergenic
GCAGCAGCCTCCACCAAATT+TGG 0.216972 tig0024251:-13274 Msa1415580:CDS
GTCGGTGGTCGGTGAGGGTT+TGG 0.228941 tig0024251:+15765 None:intergenic
TGAGTTTGCGCAAGTATTTA+TGG 0.229399 tig0024251:-13168 Msa1415580:three_prime_UTR
GCGCAAAATCTACAGCATTT+TGG 0.230172 tig0024251:+13409 None:intergenic
TCATAGGCTGCGATGGGATT+TGG 0.232647 tig0024251:-13446 Msa1415580:CDS
GCATTGGCTACTCTTGTTAT+AGG 0.258949 tig0024251:-14016 Msa1415580:CDS
CTTAATGTTGCTCGAGCTAT+TGG 0.303340 tig0024251:-13570 Msa1415580:CDS
CATAGGCTGCGATGGGATTT+GGG 0.327999 tig0024251:-13445 Msa1415580:CDS
GATGGACCGAGTGCTTTCTA+TGG 0.328546 tig0024251:-14619 Msa1415580:CDS
TATCGGGTTCCGGTCAAATA+TGG 0.330817 tig0024251:-14704 Msa1415580:CDS
AGTTTCTGACTGTAGGTTGC+TGG 0.335395 tig0024251:-13745 Msa1415580:intron
TCCGGAGGATGTGCTGATAT+CGG 0.336894 tig0024251:-14721 Msa1415580:CDS
GAGCGTGGTGCTACCAAATT+TGG 0.350164 tig0024251:+13261 None:intergenic
CTCAATAAACAACTTATAGA+TGG 0.363410 tig0024251:-14637 Msa1415580:CDS
GCAATGGATGGATTAGGATT+TGG 0.372864 tig0024251:+15438 None:intergenic
GTCTATGATGGATATCTTAA+CGG 0.377116 tig0024251:-13597 Msa1415580:CDS
CAGAGGCGTATCGAGGCATC+TGG 0.390804 tig0024251:-13627 Msa1415580:CDS
TGTTACTTGTTAGGTGTAGT+AGG 0.391570 tig0024251:-13050 Msa1415580:three_prime_UTR
CATCCTCCGGAACGGAGCTT+TGG 0.398949 tig0024251:+14733 None:intergenic
TCGGTGGTCGGTGAGGGTTT+GGG 0.400048 tig0024251:+15766 None:intergenic
CTTTCATGCAACCTGGTTTG+TGG 0.400398 tig0024251:+13652 None:intergenic
TTCCTTCCAAAGCTCCGTTC+CGG 0.404057 tig0024251:-14739 Msa1415580:CDS
GCAGAAGCAATGGATGGATT+AGG 0.405446 tig0024251:+15432 None:intergenic
CTCTGCTCTTTCATGCAACC+TGG 0.407237 tig0024251:+13645 None:intergenic
AACTGTAGGTGTTACTTGTT+AGG 0.413137 tig0024251:-13059 Msa1415580:intron
AAAAGAGTGTGTTGGGAAAG+AGG 0.414423 tig0024251:+15713 None:intergenic
ATGTGGAGATTTGTTTGAAA+GGG 0.417255 tig0024251:+15683 None:intergenic
TGAATCTCAATTCTCATAAG+AGG 0.424835 tig0024251:+13116 None:intergenic
CGGTGGTCGGTGGTCGGTGA+GGG 0.425084 tig0024251:+15760 None:intergenic
AGTTACCAAAATTCGAAAAT+GGG 0.428227 tig0024251:+15565 None:intergenic
TTTATATGTCGGCGATTCGA+AGG 0.437263 tig0024251:+15648 None:intergenic
TGACTGTAGGTTGCTGGTGG+TGG 0.438915 tig0024251:-13739 Msa1415580:intron
TGGCTTGGTCAGGTGTTTGA+TGG 0.441121 tig0024251:-14214 Msa1415580:intron
CCGGAGGATGTGCTGATATC+GGG 0.442854 tig0024251:-14720 Msa1415580:CDS
AGAGGCGTATCGAGGCATCT+GGG 0.450237 tig0024251:-13626 Msa1415580:CDS
CTCAATGCTGCCCTTGCATC+CGG 0.452615 tig0024251:-14046 Msa1415580:CDS
TTGCTGGTGGTGGCAAATGC+TGG 0.454788 tig0024251:-13729 Msa1415580:CDS
ATGCAGTGGTGCCGGATGCA+AGG 0.459353 tig0024251:+14035 None:intergenic
GCAAACAGACTATGCATTTG+CGG 0.463349 tig0024251:-14080 Msa1415580:CDS
TTTGAAGAAAAGAGTGTGTT+GGG 0.473612 tig0024251:+15706 None:intergenic
AATGTGGAGATTTGTTTGAA+AGG 0.474500 tig0024251:+15682 None:intergenic
TGCATCCGGCACCACTGCAT+TGG 0.476188 tig0024251:-14032 Msa1415580:CDS
TCTATGATGGATATCTTAAC+GGG 0.479521 tig0024251:-13596 Msa1415580:CDS
TGCAGTGGTGCCGGATGCAA+GGG 0.482277 tig0024251:+14036 None:intergenic
AGCTGTATGCAGTAAGGATC+TGG 0.483557 tig0024251:-13358 Msa1415580:CDS
GATGTGCTGATATCGGGTTC+CGG 0.494574 tig0024251:-14714 Msa1415580:CDS
CCCGATATCAGCACATCCTC+CGG 0.497125 tig0024251:+14720 None:intergenic
AGATCCTTACTGCATACAGC+TGG 0.500453 tig0024251:+13360 None:intergenic
TAAGTTGTTTATTGAGCCCA+TGG 0.501821 tig0024251:+14644 None:intergenic
TTATCCTCGAGAATGAACTT+TGG 0.503539 tig0024251:+14148 None:intergenic
AGTAGCCAATGCAGTGGTGC+CGG 0.504279 tig0024251:+14027 None:intergenic
ACTATCCTTTCAATATCCAC+TGG 0.504474 tig0024251:+14118 None:intergenic
CACAGGAGCTTCTCTGCTGA+AGG 0.507072 tig0024251:-13231 Msa1415580:CDS
AGGCGTATCGAGGCATCTGG+GGG 0.513077 tig0024251:-13624 Msa1415580:CDS
ATGGACCGAGTGCTTTCTAT+GGG 0.513585 tig0024251:-14618 Msa1415580:CDS
GCGGTGGTCGGTGGTCGGTG+AGG 0.520865 tig0024251:+15759 None:intergenic
AGAATGACCGTCGTAAATGA+AGG 0.524484 tig0024251:+15737 None:intergenic
ATGGATGGATTAGGATTTGG+AGG 0.527042 tig0024251:+15441 None:intergenic
TTTCTCATCATAGGCTGCGA+TGG 0.528638 tig0024251:-13453 Msa1415580:CDS
TATGTCGGCGATTCGAAGGG+TGG 0.528965 tig0024251:+15652 None:intergenic
TTCTCATCATAGGCTGCGAT+GGG 0.530891 tig0024251:-13452 Msa1415580:CDS
GAAGGAATGAAAGGCAAAGA+TGG 0.533012 tig0024251:-13534 Msa1415580:CDS
TGAGATTCATTTGTAACTGT+AGG 0.533426 tig0024251:-13102 Msa1415580:intron
TGCATAGTCTGTTTGCAGAA+AGG 0.536620 tig0024251:+14087 None:intergenic
TTATATGTCGGCGATTCGAA+GGG 0.537707 tig0024251:+15649 None:intergenic
CGACCCAGCTGTATGCAGTA+AGG 0.541516 tig0024251:-13364 Msa1415580:CDS
TAAATGAAGGCGGTGGTCGG+TGG 0.544330 tig0024251:+15750 None:intergenic
GTTTGAAGAAAAGAGTGTGT+TGG 0.546377 tig0024251:+15705 None:intergenic
TAAAATAAAATTAAACAAGA+AGG 0.548575 tig0024251:+13143 None:intergenic
ATTTCCCAGTGGATATTGAA+AGG 0.550709 tig0024251:-14123 Msa1415580:CDS
CTTCCAAAGCTCCGTTCCGG+AGG 0.555450 tig0024251:-14736 Msa1415580:CDS
CGTGGTGCTACCAAATTTGG+TGG 0.556204 tig0024251:+13264 None:intergenic
TGGCTACTCTTGTTATAGGA+AGG 0.559140 tig0024251:-14012 Msa1415580:intron
GAGGCGTATCGAGGCATCTG+GGG 0.559861 tig0024251:-13625 Msa1415580:CDS
CTATCCTTTCAATATCCACT+GGG 0.563023 tig0024251:+14119 None:intergenic
ATGTCAAGAGACCACAAACC+AGG 0.567267 tig0024251:-13663 Msa1415580:CDS
AGAGAAGCTCCTGTGCACTC+TGG 0.567952 tig0024251:+13239 None:intergenic
TGGCACATGGAAGGAATGAA+AGG 0.568858 tig0024251:-13543 Msa1415580:CDS
GGTCAGGTGTTTGATGGACA+CGG 0.571598 tig0024251:-14208 Msa1415580:intron
TTTCTGCATGGTAAAACAAG+AGG 0.572653 tig0024251:+15384 None:intergenic
ATTGGTGACTGGCACATGGA+AGG 0.574252 tig0024251:-13552 Msa1415580:CDS
CTCCGGAACGGAGCTTTGGA+AGG 0.575225 tig0024251:+14737 None:intergenic
TTCTGACTGTAGGTTGCTGG+TGG 0.579507 tig0024251:-13742 Msa1415580:intron
CGAAAAGCAGAAGCAATGGA+TGG 0.580021 tig0024251:+15426 None:intergenic
TCGTAAATGAAGGCGGTGGT+CGG 0.582325 tig0024251:+15747 None:intergenic
TTGCTCGAGCTATTGGTGAC+TGG 0.582496 tig0024251:-13563 Msa1415580:CDS
GAGGACGAATTTCTCATCAT+AGG 0.583587 tig0024251:-13462 Msa1415580:CDS
CTCCTGTGCACTCTGGAGCG+TGG 0.585430 tig0024251:+13246 None:intergenic
ACACATCCTCCATATTTGAC+CGG 0.585526 tig0024251:+14695 None:intergenic
AGACAATTTAGCTGTTGTTG+TGG 0.586656 tig0024251:-13307 Msa1415580:CDS
TTTGCCAAAGTTCATTCTCG+AGG 0.590449 tig0024251:-14152 Msa1415580:CDS
CAGTAAGGATCTGGTTGATG+AGG 0.591652 tig0024251:-13349 Msa1415580:CDS
TGAAGGCGGTGGTCGGTGGT+CGG 0.591726 tig0024251:+15754 None:intergenic
GAAGGGTGGTTTAACAAATG+TGG 0.593767 tig0024251:+15666 None:intergenic
TTGACGAAAAGCAGAAGCAA+TGG 0.595015 tig0024251:+15422 None:intergenic
CGAGCAGTGCTGTGCCGTCG+TGG 0.598079 tig0024251:-13699 Msa1415580:CDS
GGGTTTGGGTTTCGAAGATG+CGG 0.598198 tig0024251:+15780 None:intergenic
AGCTATTGGTGACTGGCACA+TGG 0.600561 tig0024251:-13556 Msa1415580:CDS
CACCACGCTCCAGAGTGCAC+AGG 0.602591 tig0024251:-13248 Msa1415580:CDS
TTTGGGTTTCGAAGATGCGG+TGG 0.614214 tig0024251:+15783 None:intergenic
GCTTTGGAAGGAAATCAGAC+TGG 0.619255 tig0024251:+14749 None:intergenic
GGTGCTACCAAATTTGGTGG+AGG 0.621044 tig0024251:+13267 None:intergenic
CGGGTTCCGGTCAAATATGG+AGG 0.621074 tig0024251:-14701 Msa1415580:CDS
GGAATGAAAGGCAAAGATGG+TGG 0.624005 tig0024251:-13531 Msa1415580:CDS
AGTTCAGGCTCTGCACTGAG+TGG 0.625933 tig0024251:+13507 None:intergenic
GACTACAAAACTTACTGCCG+AGG 0.627191 tig0024251:-13481 Msa1415580:CDS
TATGATGAGAAATTCGTCCT+CGG 0.637476 tig0024251:+13464 None:intergenic
GATCCTTACTGCATACAGCT+GGG 0.642529 tig0024251:+13361 None:intergenic
GGTGTGAATTGTTTCAGTGA+AGG 0.642841 tig0024251:-15486 Msa1415580:CDS
ACCGTCGTAAATGAAGGCGG+TGG 0.644318 tig0024251:+15743 None:intergenic
TATCAGCACATCCTCCGGAA+CGG 0.646071 tig0024251:+14725 None:intergenic
GAAAGAGCAGAGGCGTATCG+AGG 0.646196 tig0024251:-13634 Msa1415580:CDS
TCTGTTGAGGATGTTACTGA+AGG 0.648815 tig0024251:-14775 Msa1415580:CDS
CTTTCTGACTGTATCATATG+CGG 0.655064 tig0024251:-13016 Msa1415580:three_prime_UTR
TGAAAACAAATCATAAAACA+AGG 0.663165 tig0024251:-15823 Msa1415580:five_prime_UTR
CAGGTTGCATGAAAGAGCAG+AGG 0.671998 tig0024251:-13644 Msa1415580:CDS
TATACCCCATAGAAAGCACT+CGG 0.672295 tig0024251:+14613 None:intergenic
AACAAGAGTAGCCAATGCAG+TGG 0.673761 tig0024251:+14021 None:intergenic
GTTGTCTGATGCATCTGTTG+AGG 0.696072 tig0024251:-14788 Msa1415580:CDS
GGCGTATCGAGGCATCTGGG+GGG 0.697326 tig0024251:-13623 Msa1415580:CDS
ACCACCGCCTTCATTTACGA+CGG 0.704422 tig0024251:-15744 Msa1415580:five_prime_UTR
GGTTTCTTACAAGAGAAGCG+TGG 0.710252 tig0024251:+15349 None:intergenic
CGAGGATAAAGATTTCCCAG+TGG 0.737162 tig0024251:-14134 Msa1415580:CDS
TGGACCGAGTGCTTTCTATG+GGG 0.737710 tig0024251:-14617 Msa1415580:intron
ATGACCGTCGTAAATGAAGG+CGG 0.742021 tig0024251:+15740 None:intergenic
CAGGTGTTTGATGGACACGG+CGG 0.806389 tig0024251:-14205 Msa1415580:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTGATATTCAATAATTT+GGG - tig0024251:14826-14845 Msa1415580:intron 10.0%
!! TAAAATAAAATTAAACAAGA+AGG + tig0024251:15670-15689 None:intergenic 10.0%
!! ATTTAATGTAGATAAAAACT+AGG - tig0024251:13693-13712 Msa1415580:CDS 15.0%
!!! GATTTTGATATTCAATAATT+TGG - tig0024251:14825-14844 Msa1415580:intron 15.0%
!! GCTAATTTATAGTAAAAAAA+AGG + tig0024251:14859-14878 None:intergenic 15.0%
!!! TTTATGATTTGTTTTCACAA+CGG + tig0024251:12984-13003 None:intergenic 20.0%
!! TGAAAACAAATCATAAAACA+AGG - tig0024251:12987-13006 Msa1415580:three_prime_UTR 20.0%
!!! ATTAGGTCTTTTTTATATGT+CGG + tig0024251:13176-13195 None:intergenic 20.0%
!!! TCAGAAAAAGTGTAAAAATT+AGG + tig0024251:13193-13212 None:intergenic 20.0%
!! CAAAAGAAAAAACAAAAGAT+CGG + tig0024251:13485-13504 None:intergenic 20.0%
!!! ATAATGATGTTACTTAGTTT+TGG - tig0024251:13533-13552 Msa1415580:CDS 20.0%
!! GAAATGAAATGAAATAATGA+TGG - tig0024251:13749-13768 Msa1415580:intron 20.0%
!!! TAATTGTTGATTATGTTTTG+TGG - tig0024251:13831-13850 Msa1415580:intron 20.0%
!! ATAATCAATAATCAATCCTA+AGG + tig0024251:13870-13889 None:intergenic 20.0%
!!! TAGGATTGATTATTGATTAT+TGG - tig0024251:13870-13889 Msa1415580:intron 20.0%
!! TGATATGATAGTTGTTAAAA+TGG - tig0024251:14251-14270 Msa1415580:intron 20.0%
!! GATATGATAGTTGTTAAAAT+GGG - tig0024251:14252-14271 Msa1415580:intron 20.0%
!!! TGCTAATCTTATTTTGTAAT+TGG - tig0024251:14408-14427 Msa1415580:intron 20.0%
!!! TTTTTTTTTGAACAAACTGT+AGG - tig0024251:15737-15756 Msa1415580:five_prime_UTR 20.0%
!! TTTAACCCATTTTCGAATTT+TGG - tig0024251:13240-13259 Msa1415580:CDS 25.0%
! AGTTACCAAAATTCGAAAAT+GGG + tig0024251:13248-13267 None:intergenic 25.0%
!!! ATTTTCGAATTTTGGTAACT+GGG - tig0024251:13248-13267 Msa1415580:CDS 25.0%
!!! TTTTTGAAGTTTGGATCTTA+TGG - tig0024251:13277-13296 Msa1415580:CDS 25.0%
! GATCTTATGGTTAAGAAAAA+TGG - tig0024251:13290-13309 Msa1415580:CDS 25.0%
! AGAAAAATGGAAGAAATTGA+AGG - tig0024251:13303-13322 Msa1415580:CDS 25.0%
! TGACAAATGGAAAGAAAAAA+AGG + tig0024251:13631-13650 None:intergenic 25.0%
! AGAATGTGTAATTTAAGCTT+TGG - tig0024251:13664-13683 Msa1415580:CDS 25.0%
! ATGAAATGAAATAATGATGG+CGG - tig0024251:13752-13771 Msa1415580:intron 25.0%
!!! TATTGATTATTGGTGTTAGT+AGG - tig0024251:13880-13899 Msa1415580:intron 25.0%
!!! ATTGATTATTGGTGTTAGTA+GGG - tig0024251:13881-13900 Msa1415580:intron 25.0%
!!! ATGTTTGTGTTGACAATTTT+AGG - tig0024251:14138-14157 Msa1415580:CDS 25.0%
! CTCAATAAACAACTTATAGA+TGG - tig0024251:14173-14192 Msa1415580:CDS 25.0%
! TACAAAATAAGATTAGCATG+TGG + tig0024251:14406-14425 None:intergenic 25.0%
! TAATATGTGTTTATGCACAT+TGG + tig0024251:14457-14476 None:intergenic 25.0%
! TTACTATAAATTAGCACCAA+AGG - tig0024251:14864-14883 Msa1415580:intron 25.0%
!!! CATCTGACATTTTAATTTAC+TGG - tig0024251:15817-15836 Msa1415580:five_prime_UTR 25.0%
! TTTGAAGAAAAGAGTGTGTT+GGG + tig0024251:13107-13126 None:intergenic 30.0%
! ATGTGGAGATTTGTTTGAAA+GGG + tig0024251:13130-13149 None:intergenic 30.0%
! AATGTGGAGATTTGTTTGAA+AGG + tig0024251:13131-13150 None:intergenic 30.0%
CCAGATTGTAAAAAGCAATT+GGG + tig0024251:13219-13238 None:intergenic 30.0%
!!! CCAATTGCTTTTTACAATCT+GGG - tig0024251:13217-13236 Msa1415580:CDS 30.0%
CAGTTACCAAAATTCGAAAA+TGG + tig0024251:13249-13268 None:intergenic 30.0%
!! CATTTTCGAATTTTGGTAAC+TGG - tig0024251:13247-13266 Msa1415580:CDS 30.0%
AAAACTAGGACTAGAATAGA+AGG - tig0024251:13707-13726 Msa1415580:CDS 30.0%
TGAAATGAAATAATGATGGC+GGG - tig0024251:13753-13772 Msa1415580:intron 30.0%
AAAAGAGAATAACGACACAT+GGG + tig0024251:14306-14325 None:intergenic 30.0%
AATGAAAGAAGACAAAACGA+AGG + tig0024251:14329-14348 None:intergenic 30.0%
AAAAACTCCATAGCTAGTAA+AGG + tig0024251:14486-14505 None:intergenic 30.0%
CAGTTGACAAGAATAGATAT+AGG - tig0024251:14507-14526 Msa1415580:intron 30.0%
AAGTAACGTTATATGTCGAA+TGG - tig0024251:14576-14595 Msa1415580:intron 30.0%
CAAGAAATTTAGCATACCTT+TGG + tig0024251:14883-14902 None:intergenic 30.0%
AAAAGACATAAATCTGACTG+AGG - tig0024251:14925-14944 Msa1415580:intron 30.0%
GAGCATGAAAATGGAAATAT+GGG + tig0024251:14984-15003 None:intergenic 30.0%
AGAGCATGAAAATGGAAATA+TGG + tig0024251:14985-15004 None:intergenic 30.0%
!! GTCTATGATGGATATCTTAA+CGG - tig0024251:15213-15232 Msa1415580:intron 30.0%
!! TCTATGATGGATATCTTAAC+GGG - tig0024251:15214-15233 Msa1415580:intron 30.0%
TGAATCTCAATTCTCATAAG+AGG + tig0024251:15697-15716 None:intergenic 30.0%
TGAGATTCATTTGTAACTGT+AGG - tig0024251:15708-15727 Msa1415580:five_prime_UTR 30.0%
! GTTTGAAGAAAAGAGTGTGT+TGG + tig0024251:13108-13127 None:intergenic 35.0%
!!! CCCAATTGCTTTTTACAATC+TGG - tig0024251:13216-13235 Msa1415580:CDS 35.0%
CCCAGATTGTAAAAAGCAAT+TGG + tig0024251:13220-13239 None:intergenic 35.0%
TTTCTGCATGGTAAAACAAG+AGG + tig0024251:13429-13448 None:intergenic 35.0%
CACTACATGAAAGTGACAAA+TGG + tig0024251:13644-13663 None:intergenic 35.0%
!! TTGTGTTGACAATTTTAGGC+AGG - tig0024251:14142-14161 Msa1415580:CDS 35.0%
!! TGTGTTGACAATTTTAGGCA+GGG - tig0024251:14143-14162 Msa1415580:CDS 35.0%
TAAGTTGTTTATTGAGCCCA+TGG + tig0024251:14169-14188 None:intergenic 35.0%
AGTACTCCAATCAAGATAAG+CGG + tig0024251:14232-14251 None:intergenic 35.0%
AAAGAGAATAACGACACATG+GGG + tig0024251:14305-14324 None:intergenic 35.0%
GAAAAGAGAATAACGACACA+TGG + tig0024251:14307-14326 None:intergenic 35.0%
AATTGGTACTGCTTTACCTT+TGG - tig0024251:14425-14444 Msa1415580:intron 35.0%
TTATCCTCGAGAATGAACTT+TGG + tig0024251:14665-14684 None:intergenic 35.0%
ATTTCCCAGTGGATATTGAA+AGG - tig0024251:14687-14706 Msa1415580:CDS 35.0%
CTATCCTTTCAATATCCACT+GGG + tig0024251:14694-14713 None:intergenic 35.0%
ACTATCCTTTCAATATCCAC+TGG + tig0024251:14695-14714 None:intergenic 35.0%
!!! AATTTCTTGTCCGCGTTTTT+TGG - tig0024251:14894-14913 Msa1415580:intron 35.0%
TTGTACTTGTCCAAAAAACG+CGG + tig0024251:14907-14926 None:intergenic 35.0%
ACATAAATCTGACTGAGGTT+TGG - tig0024251:14930-14949 Msa1415580:intron 35.0%
CATAAATCTGACTGAGGTTT+GGG - tig0024251:14931-14950 Msa1415580:intron 35.0%
! TTTTGGTCTGCTACAAAAGT+TGG - tig0024251:14958-14977 Msa1415580:intron 35.0%
TGTATTTGATGTCATGTCAG+AGG + tig0024251:15009-15028 None:intergenic 35.0%
TGATTGTAGTTTCTGACTGT+AGG - tig0024251:15058-15077 Msa1415580:intron 35.0%
!! AGTTTTGTAGTCATGAGTTC+AGG + tig0024251:15321-15340 None:intergenic 35.0%
TATGATGAGAAATTCGTCCT+CGG + tig0024251:15349-15368 None:intergenic 35.0%
! AAAATGCTGTAGATTTTGCG+CGG - tig0024251:15403-15422 Msa1415580:CDS 35.0%
AGACAATTTAGCTGTTGTTG+TGG - tig0024251:15503-15522 Msa1415580:CDS 35.0%
! GAAAGAGTTGCAAAGCTTTT+TGG - tig0024251:15605-15624 Msa1415580:five_prime_UTR 35.0%
! CAAAGCTTTTTGGATAGCTT+GGG - tig0024251:15615-15634 Msa1415580:five_prime_UTR 35.0%
TGAGTTTGCGCAAGTATTTA+TGG - tig0024251:15642-15661 Msa1415580:five_prime_UTR 35.0%
!! AACTGTAGGTGTTACTTGTT+AGG - tig0024251:15751-15770 Msa1415580:five_prime_UTR 35.0%
! TGTTACTTGTTAGGTGTAGT+AGG - tig0024251:15760-15779 Msa1415580:five_prime_UTR 35.0%
CTTTCTGACTGTATCATATG+CGG - tig0024251:15794-15813 Msa1415580:five_prime_UTR 35.0%
AGAATGACCGTCGTAAATGA+AGG + tig0024251:13076-13095 None:intergenic 40.0%
! AAAAGAGTGTGTTGGGAAAG+AGG + tig0024251:13100-13119 None:intergenic 40.0%
GAAGGGTGGTTTAACAAATG+TGG + tig0024251:13147-13166 None:intergenic 40.0%
TTATATGTCGGCGATTCGAA+GGG + tig0024251:13164-13183 None:intergenic 40.0%
TTTATATGTCGGCGATTCGA+AGG + tig0024251:13165-13184 None:intergenic 40.0%
!!! GGGTCTGTGTTTTTGAAGTT+TGG - tig0024251:13268-13287 Msa1415580:CDS 40.0%
GGTGTGAATTGTTTCAGTGA+AGG - tig0024251:13324-13343 Msa1415580:CDS 40.0%
!! ATGGATGGATTAGGATTTGG+AGG + tig0024251:13372-13391 None:intergenic 40.0%
!! GCAATGGATGGATTAGGATT+TGG + tig0024251:13375-13394 None:intergenic 40.0%
TTGACGAAAAGCAGAAGCAA+TGG + tig0024251:13391-13410 None:intergenic 40.0%
! CGAACAAGAGATTTTCTGCA+TGG + tig0024251:13441-13460 None:intergenic 40.0%
!!! GAAATAATGATGGCGGGTTT+TGG - tig0024251:13759-13778 Msa1415580:intron 40.0%
!!! GGTTTTGGTTTGGTCAGATT+GGG - tig0024251:13774-13793 Msa1415580:intron 40.0%
!!! GTTTTGGTTTGGTCAGATTG+GGG - tig0024251:13775-13794 Msa1415580:intron 40.0%
TGGATTCAGTTTGCAACCTT+AGG - tig0024251:13851-13870 Msa1415580:intron 40.0%
! GGTGTTAGTAGGGTTATTTC+TGG - tig0024251:13891-13910 Msa1415580:intron 40.0%
TCTGTTGAGGATGTTACTGA+AGG - tig0024251:14035-14054 Msa1415580:CDS 40.0%
ACACATCCTCCATATTTGAC+CGG + tig0024251:14118-14137 None:intergenic 40.0%
!! TATACCCCATAGAAAGCACT+CGG + tig0024251:14200-14219 None:intergenic 40.0%
GAACTTCCAAAAGATGCCAT+CGG + tig0024251:14368-14387 None:intergenic 40.0%
ACGTTATATGTCGAATGGCT+TGG - tig0024251:14581-14600 Msa1415580:intron 40.0%
TTTGCCAAAGTTCATTCTCG+AGG - tig0024251:14658-14677 Msa1415580:CDS 40.0%
TGCATAGTCTGTTTGCAGAA+AGG + tig0024251:14726-14745 None:intergenic 40.0%
GCAAACAGACTATGCATTTG+CGG - tig0024251:14730-14749 Msa1415580:CDS 40.0%
!! GCATTGGCTACTCTTGTTAT+AGG - tig0024251:14794-14813 Msa1415580:CDS 40.0%
! TGGCTACTCTTGTTATAGGA+AGG - tig0024251:14798-14817 Msa1415580:CDS 40.0%
! ACTCTTGTTATAGGAAGGTC+AGG - tig0024251:14803-14822 Msa1415580:CDS 40.0%
!! ACTGAGGTTTGGGATCTTTT+TGG - tig0024251:14941-14960 Msa1415580:intron 40.0%
! TCAGAGGAAGAGCATGAAAA+TGG + tig0024251:14993-15012 None:intergenic 40.0%
CTTAATGTTGCTCGAGCTAT+TGG - tig0024251:15240-15259 Msa1415580:intron 40.0%
GAAGGAATGAAAGGCAAAGA+TGG - tig0024251:15276-15295 Msa1415580:intron 40.0%
GAGGACGAATTTCTCATCAT+AGG - tig0024251:15348-15367 Msa1415580:intron 40.0%
! GCGCAAAATCTACAGCATTT+TGG + tig0024251:15404-15423 None:intergenic 40.0%
!! GAGGCTTTGAAGAGAAAAAG+TGG - tig0024251:15480-15499 Msa1415580:CDS 40.0%
! GCAAAGCTTTTTGGATAGCT+TGG - tig0024251:15614-15633 Msa1415580:five_prime_UTR 40.0%
ATGACCGTCGTAAATGAAGG+CGG + tig0024251:13073-13092 None:intergenic 45.0%
! GCAGAAGCAATGGATGGATT+AGG + tig0024251:13381-13400 None:intergenic 45.0%
CGAAAAGCAGAAGCAATGGA+TGG + tig0024251:13387-13406 None:intergenic 45.0%
GGTTTCTTACAAGAGAAGCG+TGG + tig0024251:13464-13483 None:intergenic 45.0%
!!! AATGATGGCGGGTTTTGGTT+TGG - tig0024251:13764-13783 Msa1415580:intron 45.0%
!!! GGGTTTTGGTTTGGTCAGAT+TGG - tig0024251:13773-13792 Msa1415580:intron 45.0%
GTTGTCTGATGCATCTGTTG+AGG - tig0024251:14022-14041 Msa1415580:CDS 45.0%
!! GCTTTGGAAGGAAATCAGAC+TGG + tig0024251:14064-14083 None:intergenic 45.0%
TATCGGGTTCCGGTCAAATA+TGG - tig0024251:14106-14125 Msa1415580:CDS 45.0%
! ACAATTTTAGGCAGGGCCAT+GGG - tig0024251:14150-14169 Msa1415580:CDS 45.0%
ATGGACCGAGTGCTTTCTAT+GGG - tig0024251:14192-14211 Msa1415580:CDS 45.0%
CTCTCTCCGCTTATCTTGAT+TGG - tig0024251:14223-14242 Msa1415580:intron 45.0%
GTACTGCTTTACCTTTGGTG+TGG - tig0024251:14430-14449 Msa1415580:intron 45.0%
!! ATATGTCGAATGGCTTGGTC+AGG - tig0024251:14586-14605 Msa1415580:intron 45.0%
CGAGGATAAAGATTTCCCAG+TGG - tig0024251:14676-14695 Msa1415580:CDS 45.0%
AACAAGAGTAGCCAATGCAG+TGG + tig0024251:14792-14811 None:intergenic 45.0%
AGTTTCTGACTGTAGGTTGC+TGG - tig0024251:15065-15084 Msa1415580:intron 45.0%
!! TGTCGATTGCTTTTCCACGA+CGG + tig0024251:15128-15147 None:intergenic 45.0%
ATGTCAAGAGACCACAAACC+AGG - tig0024251:15147-15166 Msa1415580:intron 45.0%
! CTTTCATGCAACCTGGTTTG+TGG + tig0024251:15161-15180 None:intergenic 45.0%
TGGCACATGGAAGGAATGAA+AGG - tig0024251:15267-15286 Msa1415580:intron 45.0%
GGAATGAAAGGCAAAGATGG+TGG - tig0024251:15279-15298 Msa1415580:intron 45.0%
GACTACAAAACTTACTGCCG+AGG - tig0024251:15329-15348 Msa1415580:intron 45.0%
TTTCTCATCATAGGCTGCGA+TGG - tig0024251:15357-15376 Msa1415580:intron 45.0%
TTCTCATCATAGGCTGCGAT+GGG - tig0024251:15358-15377 Msa1415580:intron 45.0%
GATCCTTACTGCATACAGCT+GGG + tig0024251:15452-15471 None:intergenic 45.0%
AGATCCTTACTGCATACAGC+TGG + tig0024251:15453-15472 None:intergenic 45.0%
AGCTGTATGCAGTAAGGATC+TGG - tig0024251:15452-15471 Msa1415580:CDS 45.0%
! CAGTAAGGATCTGGTTGATG+AGG - tig0024251:15461-15480 Msa1415580:CDS 45.0%
! TTTGGGTTTCGAAGATGCGG+TGG + tig0024251:13030-13049 None:intergenic 50.0%
! GGGTTTGGGTTTCGAAGATG+CGG + tig0024251:13033-13052 None:intergenic 50.0%
TCGTAAATGAAGGCGGTGGT+CGG + tig0024251:13066-13085 None:intergenic 50.0%
ACCACCGCCTTCATTTACGA+CGG - tig0024251:13066-13085 Msa1415580:intron 50.0%
TTCCTTCCAAAGCTCCGTTC+CGG - tig0024251:14071-14090 Msa1415580:CDS 50.0%
TATCAGCACATCCTCCGGAA+CGG + tig0024251:14088-14107 None:intergenic 50.0%
! TCCGGAGGATGTGCTGATAT+CGG - tig0024251:14089-14108 Msa1415580:CDS 50.0%
! GATGTGCTGATATCGGGTTC+CGG - tig0024251:14096-14115 Msa1415580:CDS 50.0%
! GACAATTTTAGGCAGGGCCA+TGG - tig0024251:14149-14168 Msa1415580:CDS 50.0%
GATGGACCGAGTGCTTTCTA+TGG - tig0024251:14191-14210 Msa1415580:CDS 50.0%
TGGACCGAGTGCTTTCTATG+GGG - tig0024251:14193-14212 Msa1415580:CDS 50.0%
! GCACATCCGATGGCATCTTT+TGG - tig0024251:14359-14378 Msa1415580:intron 50.0%
TGCACATTGGTCCACACCAA+AGG + tig0024251:14444-14463 None:intergenic 50.0%
AGACGCGCCTTTACTAGCTA+TGG - tig0024251:14476-14495 Msa1415580:intron 50.0%
!! TGGCTTGGTCAGGTGTTTGA+TGG - tig0024251:14596-14615 Msa1415580:intron 50.0%
! GGTCAGGTGTTTGATGGACA+CGG - tig0024251:14602-14621 Msa1415580:intron 50.0%
TTCTGACTGTAGGTTGCTGG+TGG - tig0024251:15068-15087 Msa1415580:intron 50.0%
CTCTGCTCTTTCATGCAACC+TGG + tig0024251:15168-15187 None:intergenic 50.0%
CAGGTTGCATGAAAGAGCAG+AGG - tig0024251:15166-15185 Msa1415580:intron 50.0%
! TTGCTCGAGCTATTGGTGAC+TGG - tig0024251:15247-15266 Msa1415580:intron 50.0%
! AGCTATTGGTGACTGGCACA+TGG - tig0024251:15254-15273 Msa1415580:intron 50.0%
! ATTGGTGACTGGCACATGGA+AGG - tig0024251:15258-15277 Msa1415580:intron 50.0%
TCATAGGCTGCGATGGGATT+TGG - tig0024251:15364-15383 Msa1415580:CDS 50.0%
CATAGGCTGCGATGGGATTT+GGG - tig0024251:15365-15384 Msa1415580:CDS 50.0%
! GGTGCTACCAAATTTGGTGG+AGG + tig0024251:15546-15565 None:intergenic 50.0%
!! CGTGGTGCTACCAAATTTGG+TGG + tig0024251:15549-15568 None:intergenic 50.0%
!! GAGCGTGGTGCTACCAAATT+TGG + tig0024251:15552-15571 None:intergenic 50.0%
TAAATGAAGGCGGTGGTCGG+TGG + tig0024251:13063-13082 None:intergenic 55.0%
ACCGTCGTAAATGAAGGCGG+TGG + tig0024251:13070-13089 None:intergenic 55.0%
TATGTCGGCGATTCGAAGGG+TGG + tig0024251:13161-13180 None:intergenic 55.0%
CCCGATATCAGCACATCCTC+CGG + tig0024251:14093-14112 None:intergenic 55.0%
! CCGGAGGATGTGCTGATATC+GGG - tig0024251:14090-14109 Msa1415580:CDS 55.0%
CGGGTTCCGGTCAAATATGG+AGG - tig0024251:14109-14128 Msa1415580:CDS 55.0%
CTCCTGTTCTGCACATCCGA+TGG - tig0024251:14349-14368 Msa1415580:intron 55.0%
TGCCATCGGATGTGCAGAAC+AGG + tig0024251:14354-14373 None:intergenic 55.0%
! CAGGTGTTTGATGGACACGG+CGG - tig0024251:14605-14624 Msa1415580:intron 55.0%
CTCAATGCTGCCCTTGCATC+CGG - tig0024251:14764-14783 Msa1415580:CDS 55.0%
AGTAGCCAATGCAGTGGTGC+CGG + tig0024251:14786-14805 None:intergenic 55.0%
TGACTGTAGGTTGCTGGTGG+TGG - tig0024251:15071-15090 Msa1415580:intron 55.0%
!! TTGCTGGTGGTGGCAAATGC+TGG - tig0024251:15081-15100 Msa1415580:intron 55.0%
! GAAAGAGCAGAGGCGTATCG+AGG - tig0024251:15176-15195 Msa1415580:intron 55.0%
! AGAGGCGTATCGAGGCATCT+GGG - tig0024251:15184-15203 Msa1415580:intron 55.0%
TCTGGGGGGTCTGTCTATGA+TGG - tig0024251:15201-15220 Msa1415580:intron 55.0%
AGTTCAGGCTCTGCACTGAG+TGG + tig0024251:15306-15325 None:intergenic 55.0%
CGACCCAGCTGTATGCAGTA+AGG - tig0024251:15446-15465 Msa1415580:CDS 55.0%
GCAGCAGCCTCCACCAAATT+TGG - tig0024251:15536-15555 Msa1415580:five_prime_UTR 55.0%
AGAGAAGCTCCTGTGCACTC+TGG + tig0024251:15574-15593 None:intergenic 55.0%
CACAGGAGCTTCTCTGCTGA+AGG - tig0024251:15579-15598 Msa1415580:five_prime_UTR 55.0%
TCGGTGGTCGGTGAGGGTTT+GGG + tig0024251:13047-13066 None:intergenic 60.0%
!! CTCCGGAACGGAGCTTTGGA+AGG + tig0024251:14076-14095 None:intergenic 60.0%
CTTCCAAAGCTCCGTTCCGG+AGG - tig0024251:14074-14093 Msa1415580:CDS 60.0%
CATCCTCCGGAACGGAGCTT+TGG + tig0024251:14080-14099 None:intergenic 60.0%
! TGCAGTGGTGCCGGATGCAA+GGG + tig0024251:14777-14796 None:intergenic 60.0%
! ATGCAGTGGTGCCGGATGCA+AGG + tig0024251:14778-14797 None:intergenic 60.0%
TGCATCCGGCACCACTGCAT+TGG - tig0024251:14778-14797 Msa1415580:CDS 60.0%
! CAGAGGCGTATCGAGGCATC+TGG - tig0024251:15183-15202 Msa1415580:intron 60.0%
! GAGGCGTATCGAGGCATCTG+GGG - tig0024251:15185-15204 Msa1415580:intron 60.0%
! AGGCGTATCGAGGCATCTGG+GGG - tig0024251:15186-15205 Msa1415580:intron 60.0%
! CTGTAGATTTTGCGCGGCGC+AGG - tig0024251:15409-15428 Msa1415580:CDS 60.0%
GTCGGTGGTCGGTGAGGGTT+TGG + tig0024251:13048-13067 None:intergenic 65.0%
TGAAGGCGGTGGTCGGTGGT+CGG + tig0024251:13059-13078 None:intergenic 65.0%
! GGCGTATCGAGGCATCTGGG+GGG - tig0024251:15187-15206 Msa1415580:intron 65.0%
! TTTTGCGCGGCGCAGGCTTC+AGG - tig0024251:15416-15435 Msa1415580:CDS 65.0%
CACCACGCTCCAGAGTGCAC+AGG - tig0024251:15562-15581 Msa1415580:five_prime_UTR 65.0%
!! CTCCTGTGCACTCTGGAGCG+TGG + tig0024251:15567-15586 None:intergenic 65.0%
CGGTGGTCGGTGGTCGGTGA+GGG + tig0024251:13053-13072 None:intergenic 70.0%
CGAGCAGTGCTGTGCCGTCG+TGG - tig0024251:15111-15130 Msa1415580:intron 70.0%
GCGGTGGTCGGTGGTCGGTG+AGG + tig0024251:13054-13073 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
tig0024251 gene 12966 15866 12966 ID=Msa1415580;Name=Msa1415580
tig0024251 mRNA 12966 15866 12966 ID=Msa1415580-mRNA-1;Parent=Msa1415580;Name=Msa1415580-mRNA-1;_AED=0.12;_eAED=0.12;_QI=343|1|1|1|0.75|0.6|5|193|382
tig0024251 exon 12966 13073 12966 ID=Msa1415580-mRNA-1:exon:22556;Parent=Msa1415580-mRNA-1
tig0024251 exon 13103 13752 13103 ID=Msa1415580-mRNA-1:exon:22555;Parent=Msa1415580-mRNA-1
tig0024251 exon 14013 14224 14013 ID=Msa1415580-mRNA-1:exon:22554;Parent=Msa1415580-mRNA-1
tig0024251 exon 14618 14824 14618 ID=Msa1415580-mRNA-1:exon:22553;Parent=Msa1415580-mRNA-1
tig0024251 exon 15359 15866 15359 ID=Msa1415580-mRNA-1:exon:22552;Parent=Msa1415580-mRNA-1
tig0024251 five_prime_UTR 15524 15866 15524 ID=Msa1415580-mRNA-1:five_prime_utr;Parent=Msa1415580-mRNA-1
tig0024251 CDS 15359 15523 15359 ID=Msa1415580-mRNA-1:cds;Parent=Msa1415580-mRNA-1
tig0024251 CDS 14618 14824 14618 ID=Msa1415580-mRNA-1:cds;Parent=Msa1415580-mRNA-1
tig0024251 CDS 14013 14224 14013 ID=Msa1415580-mRNA-1:cds;Parent=Msa1415580-mRNA-1
tig0024251 CDS 13188 13752 13188 ID=Msa1415580-mRNA-1:cds;Parent=Msa1415580-mRNA-1
tig0024251 three_prime_UTR 13103 13187 13103 ID=Msa1415580-mRNA-1:three_prime_utr;Parent=Msa1415580-mRNA-1
tig0024251 three_prime_UTR 12966 13073 12966 ID=Msa1415580-mRNA-1:three_prime_utr;Parent=Msa1415580-mRNA-1
Gene Sequence

>Msa1415580

ATGGAAGAAATTGAAGGTGTGAATTGTTTCAGTGAAGGTAGAAGCTCTAGTGAGAGTCGACCTCCAAATCCTAATCCATCCATTGCTTCTGCTTTTCGTCAATGTCAACGCAATGATCCTCTTGTTTTACCATGCAGAAAATCTCTTGTTCGCCACGCTTCTCTTCAGACGAAAATGAAGTTGTCTGATGCATCTGTTGAGGATGTTACTGAAGGTTGCCAGTCTGATTTCCTTCCAAAGCTCCGTTCCGGAGGATGTGCTGATATCGGGTTCCGGTCAAATATGGAGGATGTGTATGTTTGTGTTGACAATTTTAGGCAGGGCCATGGGCTCAATAAACAACTTATAGATGGACCGAGTGCTTTCTATGGGGTGTTTGATGGACACGGCGGAAAGCATGCTGCTGACTTTGCTTGCAATCATTTGCCAAAGTTCATTCTCGAGGATAAAGATTTCCCAGTGGATATTGAAAGGATAGTTGCTTCTGCCTTTCTGCAAACAGACTATGCATTTGCGGAAGCTTGTTCTCTCAATGCTGCCCTTGCATCCGGCACCACTGCATTGGCTACTCTTGTTATAGGAAGGTTGCTGGTGGTGGCAAATGCTGGAGATTGTCGAGCAGTGCTGTGCCGTCGTGGAAAAGCAATCGACATGTCAAGAGACCACAAACCAGGTTGCATGAAAGAGCAGAGGCGTATCGAGGCATCTGGGGGGTCTGTCTATGATGGATATCTTAACGGGCAACTTAATGTTGCTCGAGCTATTGGTGACTGGCACATGGAAGGAATGAAAGGCAAAGATGGTGGACCACTCAGTGCAGAGCCTGAACTCATGACTACAAAACTTACTGCCGAGGACGAATTTCTCATCATAGGCTGCGATGGGATTTGGGATGTTTTTAGAAGCCAAAATGCTGTAGATTTTGCGCGGCGCAGGCTTCAGGTGCATAACGACCCAGCTGTATGCAGTAAGGATCTGGTTGATGAGGCTTTGAAGAGAAAAAGTGGAGACAATTTAGCTGTTGTTGTGGTTTGCTTTCAGCAGCAGCCTCCACCAAATTTGGTAGCACCACGCTCCAGAGTGCACAGGAGCTTCTCTGCTGAAGGTTTGAAAGAGTTGCAAAGCTTTTTGGATAGCTTGGGAAAATGA
Protein sequence

>Msa1415580

MEEIEGVNCFSEGRSSSESRPPNPNPSIASAFRQCQRNDPLVLPCRKSLVRHASLQTKMKLSDASVEDVTEGCQSDFLPKLRSGGCADIGFRSNMEDVYVCVDNFRQGHGLNKQLIDGPSAFYGVFDGHGGKHAADFACNHLPKFILEDKDFPVDIERIVASAFLQTDYAFAEACSLNAALASGTTALATLVIGRLLVVANAGDCRAVLCRRGKAIDMSRDHKPGCMKEQRRIEASGGSVYDGYLNGQLNVARAIGDWHMEGMKGKDGGPLSAEPELMTTKLTAEDEFLIIGCDGIWDVFRSQNAVDFARRRLQVHNDPAVCSKDLVDEALKRKSGDNLAVVVVCFQQQPPPNLVAPRSRVHRSFSAEGLKELQSFLDSLGK*