AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1371380

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1371380 MtrunA17_Chr3g0138281 97.644 382 8 1 1 382 1 381 0.0 766
Msa1371380 MtrunA17_Chr4g0065141 58.497 306 123 3 76 378 79 383 4.47e-127 370
Msa1371380 MtrunA17_Chr5g0407161 54.575 306 133 4 74 378 77 377 1.66e-112 333
Msa1371380 MtrunA17_Chr3g0144111 54.577 284 104 4 95 378 1 259 1.08e-99 296
Msa1371380 MtrunA17_Chr1g0194191 45.402 348 160 8 49 378 50 385 1.37e-89 275
Msa1371380 MtrunA17_Chr2g0293811 43.491 338 174 7 55 379 34 367 2.91e-85 263
Msa1371380 MtrunA17_Chr1g0150451 36.162 271 147 8 80 346 75 323 1.80e-40 146
Msa1371380 MtrunA17_Chr1g0150071 29.714 350 177 8 61 357 5 338 2.85e-40 146
Msa1371380 MtrunA17_Chr1g0171571 35.106 282 148 9 77 344 382 642 3.08e-40 150
Msa1371380 MtrunA17_Chr3g0136811 29.944 354 175 9 59 357 3 338 7.12e-40 145
Msa1371380 MtrunA17_Chr3g0136711 35.055 271 150 8 80 346 32 280 1.24e-38 140
Msa1371380 MtrunA17_Chr4g0001761 30.530 321 156 7 81 347 22 329 1.45e-38 141
Msa1371380 MtrunA17_Chr5g0430481 35.926 270 138 7 90 348 131 376 1.76e-38 141
Msa1371380 MtrunA17_Chr6g0483411 31.392 309 170 8 66 348 28 320 1.21e-37 138
Msa1371380 MtrunA17_Chr3g0105371 36.090 266 141 7 90 347 95 339 1.22e-37 138
Msa1371380 MtrunA17_Chr4g0068971 45.679 162 76 4 224 378 110 266 1.71e-37 136
Msa1371380 MtrunA17_Chr3g0105351 35.821 268 139 8 90 347 71 315 3.85e-37 136
Msa1371380 MtrunA17_Chr1g0212961 35.227 264 143 8 90 350 103 341 8.18e-37 136
Msa1371380 MtrunA17_Chr3g0115851 35.983 239 134 5 114 347 58 282 2.90e-36 133
Msa1371380 MtrunA17_Chr5g0426141 35.271 258 139 8 95 347 48 282 1.60e-35 131
Msa1371380 MtrunA17_Chr3g0126511 32.632 285 165 7 69 349 82 343 2.81e-35 132
Msa1371380 MtrunA17_Chr2g0279151 36.797 231 127 6 122 347 65 281 1.41e-34 129
Msa1371380 MtrunA17_Chr4g0050381 35.065 231 131 5 122 347 61 277 2.17e-32 123
Msa1371380 MtrunA17_Chr2g0317021 34.167 240 139 6 113 347 108 333 3.28e-31 121
Msa1371380 MtrunA17_Chr2g0299981 29.467 319 160 9 89 349 227 538 7.44e-30 120
Msa1371380 MtrunA17_Chr3g0127091 27.152 302 171 7 79 343 57 346 8.57e-29 115
Msa1371380 MtrunA17_Chr1g0150781 29.870 308 160 10 90 349 247 546 9.10e-29 117
Msa1371380 MtrunA17_Chr8g0360351 33.195 241 140 7 113 347 57 282 5.45e-28 111
Msa1371380 MtrunA17_Chr4g0070871 29.836 305 159 9 84 344 208 501 8.63e-28 114
Msa1371380 MtrunA17_Chr3g0112291 32.184 261 135 7 119 344 120 373 9.17e-28 112
Msa1371380 MtrunA17_Chr4g0051661 33.824 272 147 10 83 346 151 397 5.55e-27 110
Msa1371380 MtrunA17_Chr1g0158781 33.333 285 151 9 81 344 111 377 2.06e-26 108
Msa1371380 MtrunA17_Chr3g0135961 29.710 276 151 7 110 349 274 542 2.65e-26 110
Msa1371380 MtrunA17_Chr5g0396731 33.981 206 118 4 122 316 107 305 2.32e-25 105
Msa1371380 MtrunA17_Chr8g0389921 28.617 311 174 8 84 363 150 443 6.40e-25 105
Msa1371380 MtrunA17_Chr5g0435721 34.112 214 112 6 122 316 223 426 2.33e-24 104
Msa1371380 MtrunA17_Chr7g0263281 27.778 288 147 12 118 349 66 348 2.22e-18 85.9
Msa1371380 MtrunA17_Chr2g0295401 28.938 273 139 13 124 350 94 357 2.58e-18 85.5
Msa1371380 MtrunA17_Chr1g0211251 27.915 283 143 12 118 344 76 353 6.46e-18 84.7
Msa1371380 MtrunA17_Chr4g0066371 29.434 265 133 11 122 344 86 338 1.01e-17 84.0
Msa1371380 MtrunA17_Chr4g0067711 27.097 310 169 13 92 350 53 356 1.02e-17 83.6
Msa1371380 MtrunA17_Chr3g0089721 29.075 227 121 6 122 311 68 291 1.07e-17 83.6
Msa1371380 MtrunA17_Chr3g0089711 28.384 229 124 6 120 311 66 291 2.66e-17 82.4
Msa1371380 MtrunA17_Chr7g0256751 27.490 251 141 8 122 337 69 313 4.16e-17 82.4
Msa1371380 MtrunA17_Chr8g0371321 26.333 300 156 13 118 361 74 364 5.70e-17 81.6
Msa1371380 MtrunA17_Chr1g0151781 30.045 223 109 8 121 303 67 282 5.82e-17 81.3
Msa1371380 MtrunA17_Chr7g0250061 26.351 296 154 12 121 361 75 361 6.84e-17 81.6
Msa1371380 MtrunA17_Chr1g0213351 26.481 287 149 12 121 351 79 359 1.05e-16 80.9
Msa1371380 MtrunA17_Chr3g0133801 27.881 269 152 9 117 360 59 310 3.09e-16 79.7
Msa1371380 MtrunA17_Chr1g0192421 28.400 250 129 10 122 331 89 328 6.93e-16 78.2
Msa1371380 MtrunA17_Chr1g0181901 28.395 243 121 11 118 319 71 301 4.30e-15 76.3
Msa1371380 MtrunA17_Chr2g0294291 28.627 255 141 10 114 332 66 315 8.44e-15 75.1
Msa1371380 MtrunA17_Chr5g0394181 29.218 243 147 10 122 346 362 597 1.06e-14 75.5
Msa1371380 MtrunA17_Chr7g0272061 28.195 266 127 11 122 334 91 345 3.83e-14 73.2
Msa1371380 MtrunA17_Chr7g0249421 35.417 144 69 4 183 308 206 343 4.46e-14 73.6
Msa1371380 MtrunA17_Chr7g0256771 28.634 227 120 7 122 310 70 292 5.22e-14 72.4
Msa1371380 MtrunA17_Chr7g0243571 27.208 283 146 10 122 350 92 368 1.35e-13 72.0
Msa1371380 MtrunA17_Chr7g0238501 26.667 225 126 7 121 306 262 486 3.39e-13 70.9
Msa1371380 MtrunA17_Chr7g0221691 35.172 145 70 5 183 309 231 369 8.50e-13 69.3
Msa1371380 MtrunA17_Chr1g0209451 29.278 263 137 13 121 357 67 306 2.38e-12 68.2
Msa1371380 MtrunA17_Chr7g0225501 30.698 215 108 12 117 303 79 280 3.87e-11 64.3
Msa1371380 MtrunA17_Chr4g0004971 26.991 226 113 8 120 300 76 294 4.49e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1371380 AT2G25620.1 66.244 394 117 7 1 381 1 391 0.0 510
Msa1371380 AT2G25620.2 66.244 394 117 7 1 381 1 391 0.0 510
Msa1371380 AT2G33700.1 55.556 306 131 4 74 378 76 377 2.05e-114 338
Msa1371380 AT3G51470.1 52.581 310 138 3 71 380 61 361 1.15e-109 326
Msa1371380 AT3G51470.2 52.581 310 138 3 71 380 17 317 1.19e-109 324
Msa1371380 AT3G62260.1 48.734 316 145 6 76 378 71 382 5.52e-90 276
Msa1371380 AT3G62260.2 48.734 316 145 6 76 378 72 383 6.55e-90 276
Msa1371380 AT1G48040.1 46.006 313 156 5 75 377 71 380 1.41e-83 259
Msa1371380 AT3G62260.3 48.485 297 136 6 95 378 1 293 1.93e-82 254
Msa1371380 AT3G17250.1 42.902 317 163 6 75 378 110 421 1.08e-74 238
Msa1371380 AT5G24940.1 36.531 271 146 8 80 346 31 279 3.23e-42 153
Msa1371380 AT5G10740.1 37.269 271 144 8 80 346 31 279 6.36e-42 150
Msa1371380 AT5G10740.2 37.269 271 144 8 80 346 31 279 6.36e-42 150
Msa1371380 AT4G31750.1 36.531 271 146 8 80 346 31 279 6.22e-40 144
Msa1371380 AT4G31750.3 36.531 271 146 8 80 346 31 279 6.22e-40 144
Msa1371380 AT4G31750.2 36.531 271 146 8 80 346 31 279 6.65e-40 144
Msa1371380 AT2G25070.1 28.895 353 179 9 61 358 5 340 6.75e-40 145
Msa1371380 AT2G25070.2 28.895 353 179 9 61 358 5 340 6.75e-40 145
Msa1371380 AT5G53140.1 33.456 272 155 7 81 348 100 349 1.20e-39 146
Msa1371380 AT5G53140.2 33.456 272 155 7 81 348 100 349 1.20e-39 146
Msa1371380 AT4G31860.1 29.969 327 161 7 80 351 21 334 5.16e-39 143
Msa1371380 AT1G78200.5 35.498 231 133 3 122 347 19 238 1.50e-37 135
Msa1371380 AT1G78200.4 35.498 231 133 3 122 347 19 238 1.50e-37 135
Msa1371380 AT2G20630.1 33.721 258 143 6 95 347 45 279 2.08e-37 136
Msa1371380 AT1G78200.1 35.498 231 133 3 122 347 64 283 3.66e-37 136
Msa1371380 AT1G78200.3 35.498 231 133 3 122 347 64 283 3.66e-37 136
Msa1371380 AT1G78200.2 35.498 231 133 3 122 347 64 283 3.66e-37 136
Msa1371380 AT2G20630.2 33.463 257 143 6 95 346 45 278 3.98e-37 136
Msa1371380 AT2G30020.1 36.431 269 140 9 90 348 147 394 8.35e-37 138
Msa1371380 AT4G28400.3 34.496 258 141 6 95 347 27 261 9.60e-37 134
Msa1371380 AT4G28400.1 34.496 258 141 6 95 347 49 283 1.92e-36 134
Msa1371380 AT4G31860.3 31.690 284 131 6 80 313 21 291 6.22e-36 133
Msa1371380 AT2G40860.4 32.404 287 158 9 75 347 287 551 7.70e-36 138
Msa1371380 AT2G40860.1 32.746 284 155 9 75 344 385 646 9.85e-36 138
Msa1371380 AT1G43900.1 34.066 273 151 9 80 347 121 369 2.20e-35 133
Msa1371380 AT1G18030.1 31.944 288 152 9 88 348 80 350 3.27e-35 132
Msa1371380 AT1G07160.1 35.338 266 140 8 90 344 130 374 9.61e-35 132
Msa1371380 AT1G22280.1 35.271 258 139 9 95 347 47 281 1.18e-33 127
Msa1371380 AT3G15260.2 34.855 241 136 6 113 347 64 289 1.20e-33 127
Msa1371380 AT3G15260.1 34.855 241 136 6 113 347 64 289 1.20e-33 127
Msa1371380 AT1G34750.2 34.468 235 134 6 116 344 59 279 6.70e-33 124
Msa1371380 AT1G34750.1 34.468 235 134 6 116 344 59 279 6.70e-33 124
Msa1371380 AT1G34750.3 34.468 235 134 6 116 344 59 279 6.70e-33 124
Msa1371380 AT1G67820.2 38.342 193 100 4 121 306 114 294 3.41e-32 125
Msa1371380 AT1G67820.1 38.342 193 100 4 121 306 150 330 4.14e-32 126
Msa1371380 AT2G40180.1 34.182 275 148 8 90 351 136 390 4.25e-32 125
Msa1371380 AT4G31860.2 30.970 268 122 6 80 297 21 275 2.05e-31 120
Msa1371380 AT1G22280.3 34.470 264 139 10 95 347 47 287 3.41e-31 120
Msa1371380 AT2G40860.2 31.387 274 152 9 75 334 385 636 5.46e-31 124
Msa1371380 AT2G29380.1 32.226 301 147 12 82 344 77 358 1.17e-28 115
Msa1371380 AT1G07430.1 31.890 254 144 8 74 310 114 355 1.40e-28 116
Msa1371380 AT4G26080.1 30.201 298 152 10 90 344 136 420 3.93e-28 114
Msa1371380 AT2G34740.1 31.801 261 146 9 95 348 101 336 6.32e-28 112
Msa1371380 AT5G51760.2 30.392 306 152 10 89 344 85 379 9.80e-28 113
Msa1371380 AT5G51760.1 30.392 306 152 10 89 344 115 409 1.36e-27 113
Msa1371380 AT5G59220.1 35.000 240 122 8 90 310 119 343 4.32e-27 111
Msa1371380 AT1G18030.2 31.933 238 119 8 88 299 80 300 4.43e-27 109
Msa1371380 AT1G72770.4 29.841 315 159 9 90 349 197 504 2.33e-26 110
Msa1371380 AT1G72770.1 29.841 315 159 9 90 349 197 504 2.33e-26 110
Msa1371380 AT1G72770.3 29.841 315 159 9 90 349 197 504 2.33e-26 110
Msa1371380 AT1G17550.1 28.615 325 163 8 84 349 190 504 2.88e-26 110
Msa1371380 AT1G17550.2 28.615 325 163 8 84 349 190 504 2.88e-26 110
Msa1371380 AT4G27800.1 27.019 322 178 8 81 361 58 363 3.09e-26 108
Msa1371380 AT3G11410.1 32.946 258 132 9 78 310 102 343 3.18e-26 108
Msa1371380 AT5G57050.1 29.373 303 153 11 90 344 120 409 3.75e-26 109
Msa1371380 AT5G57050.2 29.373 303 153 11 90 344 80 369 3.79e-26 108
Msa1371380 AT4G28400.2 34.286 210 114 5 95 299 49 239 6.39e-26 105
Msa1371380 AT1G34750.4 33.158 190 111 5 116 299 59 238 2.83e-22 95.1
Msa1371380 AT2G40860.3 31.897 232 121 9 75 290 385 595 1.32e-21 97.1
Msa1371380 AT2G34740.3 32.075 212 116 8 95 299 97 287 2.13e-21 94.0
Msa1371380 AT2G34740.2 32.075 212 116 8 95 299 115 305 2.27e-21 94.4
Msa1371380 AT4G08260.1 30.859 256 129 10 95 348 1 210 2.67e-21 91.7
Msa1371380 AT4G27800.2 26.531 294 159 8 81 333 58 335 2.19e-20 91.7
Msa1371380 AT4G27800.3 27.465 284 148 9 81 322 58 325 7.89e-20 89.7
Msa1371380 AT5G02760.1 31.780 236 113 11 121 319 71 295 1.54e-19 89.7
Msa1371380 AT5G59220.2 37.097 186 90 7 90 256 119 296 1.04e-18 86.3
Msa1371380 AT3G12620.2 28.975 283 140 13 118 344 78 355 2.76e-18 85.9
Msa1371380 AT3G12620.1 28.975 283 140 13 118 344 78 355 2.76e-18 85.9
Msa1371380 AT2G20050.2 28.625 269 130 9 122 344 143 395 2.78e-17 84.3
Msa1371380 AT2G20050.1 28.625 269 130 9 122 344 143 395 2.83e-17 84.3
Msa1371380 AT3G63340.8 29.496 278 122 12 149 366 198 461 8.72e-17 82.8
Msa1371380 AT3G63340.5 29.496 278 122 12 149 366 198 461 8.72e-17 82.8
Msa1371380 AT3G63340.1 29.496 278 122 12 149 366 198 461 8.72e-17 82.8
Msa1371380 AT3G63340.7 29.496 278 122 12 149 366 198 461 8.80e-17 82.8
Msa1371380 AT3G63340.6 29.496 278 122 12 149 366 198 461 9.69e-17 82.8
Msa1371380 AT3G63340.3 29.496 278 122 12 149 366 198 461 9.69e-17 82.8
Msa1371380 AT3G63340.10 29.496 278 122 12 149 366 198 461 9.69e-17 82.8
Msa1371380 AT3G63340.4 29.496 278 122 12 149 366 198 461 9.69e-17 82.8
Msa1371380 AT3G63340.11 29.496 278 122 12 149 366 198 461 1.04e-16 82.4
Msa1371380 AT5G01700.1 28.315 279 143 11 120 352 27 294 1.15e-16 80.9
Msa1371380 AT3G63340.12 29.496 278 122 12 149 366 198 461 1.33e-16 81.6
Msa1371380 AT4G33920.1 26.027 292 156 12 114 348 52 340 1.97e-16 80.5
Msa1371380 AT1G47380.1 29.104 268 144 10 117 357 57 305 2.42e-16 80.5
Msa1371380 AT5G01700.2 28.315 279 143 11 120 352 76 343 3.59e-16 79.7
Msa1371380 AT3G55050.1 27.273 286 147 13 118 347 79 359 6.17e-16 79.0
Msa1371380 AT3G55050.2 27.273 286 147 13 118 347 79 359 6.17e-16 79.0
Msa1371380 AT3G16800.2 31.556 225 112 9 121 310 92 309 6.91e-16 78.6
Msa1371380 AT3G16800.1 31.556 225 112 9 121 310 92 309 6.91e-16 78.6
Msa1371380 AT3G16800.3 31.556 225 112 9 121 310 92 309 6.91e-16 78.6
Msa1371380 AT3G16800.4 37.415 147 67 5 183 310 74 214 7.88e-16 77.0
Msa1371380 AT3G16800.6 37.415 147 67 5 183 310 74 214 7.88e-16 77.0
Msa1371380 AT3G05640.2 26.756 299 154 13 81 332 48 328 1.99e-15 77.4
Msa1371380 AT3G05640.1 26.756 299 154 13 81 332 48 328 1.99e-15 77.4
Msa1371380 AT3G05640.3 26.756 299 154 13 81 332 48 328 1.99e-15 77.4
Msa1371380 AT3G51370.1 25.088 283 151 11 118 344 74 351 2.31e-15 77.4
Msa1371380 AT3G51370.3 25.088 283 151 11 118 344 74 351 2.31e-15 77.4
Msa1371380 AT3G17090.1 29.032 248 131 9 121 331 83 322 3.32e-15 77.0
Msa1371380 AT3G17090.2 28.926 242 127 9 121 325 83 316 4.15e-15 75.9
Msa1371380 AT4G38520.2 25.795 283 153 11 118 346 76 355 4.30e-15 76.6
Msa1371380 AT4G38520.1 25.795 283 153 11 118 346 76 355 4.30e-15 76.6
Msa1371380 AT4G32950.1 28.641 206 118 6 121 303 71 270 8.21e-15 75.1
Msa1371380 AT3G63320.2 28.571 273 119 12 151 357 52 314 1.35e-14 75.1
Msa1371380 AT1G09160.2 30.315 254 132 11 117 344 61 295 1.53e-14 75.1
Msa1371380 AT1G09160.1 30.315 254 132 11 117 344 61 295 1.53e-14 75.1
Msa1371380 AT5G27930.1 27.600 250 115 11 107 308 82 313 2.39e-14 74.3
Msa1371380 AT5G27930.2 27.600 250 115 11 107 308 82 313 2.39e-14 74.3
Msa1371380 AT5G57050.3 30.256 195 104 8 90 258 120 308 2.97e-14 73.2
Msa1371380 AT3G63320.1 26.706 337 134 14 124 357 71 397 3.51e-14 73.9
Msa1371380 AT1G72770.2 31.220 205 103 6 90 256 197 401 5.43e-14 73.2
Msa1371380 AT1G72770.5 31.220 205 103 6 90 256 197 401 5.43e-14 73.2
Msa1371380 AT1G22280.2 35.616 146 82 5 95 240 47 180 1.18e-13 69.7
Msa1371380 AT5G66080.1 26.855 283 146 11 118 344 77 354 1.36e-13 72.0
Msa1371380 AT5G26010.1 26.493 268 148 10 124 353 75 331 2.64e-13 70.9
Msa1371380 AT3G63340.2 33.918 171 84 8 212 366 337 494 4.76e-13 71.2
Msa1371380 AT1G79630.6 32.275 189 91 8 183 346 111 287 7.78e-13 69.7
Msa1371380 AT1G79630.3 32.275 189 91 8 183 346 111 287 7.78e-13 69.7
Msa1371380 AT1G79630.4 32.275 189 91 8 183 346 111 287 7.78e-13 69.7
Msa1371380 AT1G79630.5 32.275 189 91 8 183 346 111 287 7.78e-13 69.7
Msa1371380 AT1G79630.2 32.292 192 93 8 183 349 151 330 9.25e-13 69.7
Msa1371380 AT1G79630.7 32.292 192 93 8 183 349 151 330 9.25e-13 69.7
Msa1371380 AT1G16220.1 29.515 227 119 10 148 346 161 374 1.26e-12 69.3
Msa1371380 AT1G79630.8 32.275 189 91 8 183 346 217 393 1.31e-12 69.3
Msa1371380 AT1G79630.1 32.275 189 91 8 183 346 221 397 1.38e-12 69.3
Msa1371380 AT5G06750.3 25.357 280 152 10 120 344 80 357 1.89e-12 68.6
Msa1371380 AT5G06750.1 25.357 280 152 10 120 344 80 357 1.89e-12 68.6
Msa1371380 AT5G19280.1 27.419 248 147 10 123 346 339 577 3.26e-12 68.6
Msa1371380 AT3G02750.2 34.507 142 69 5 183 306 215 350 5.05e-12 67.8
Msa1371380 AT3G02750.1 34.507 142 69 5 183 306 215 350 5.05e-12 67.8
Msa1371380 AT4G32950.2 28.141 199 114 6 121 296 71 263 1.04e-11 65.5
Msa1371380 AT3G16560.1 29.054 148 83 4 181 306 273 420 2.67e-11 65.5
Msa1371380 AT3G16560.4 29.054 148 83 4 181 306 273 420 2.67e-11 65.5
Msa1371380 AT3G16560.2 29.054 148 83 4 181 306 273 420 2.67e-11 65.5
Msa1371380 AT3G16560.3 29.054 148 83 4 181 306 273 420 2.68e-11 65.5

Find 126 sgRNAs with CRISPR-Local

Find 222 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGTTACCAAAATTTGAAAA+TGG 0.195453 tig0019197:+132488 None:intergenic
GCAGCAGCCTCCACCAAATT+TGG 0.216972 tig0019197:-130198 Msa1371380:CDS
GTCGGTGGTCGGTGAGGGTT+TGG 0.228941 tig0019197:+132689 None:intergenic
TGAGTTTGCGCAAGTATTTA+TGG 0.229399 tig0019197:-130092 Msa1371380:three_prime_UTR
GCGCAAAATCTACAGCATTT+TGG 0.230172 tig0019197:+130333 None:intergenic
TCATAGGCTGCGATGGGATT+TGG 0.232647 tig0019197:-130370 Msa1371380:CDS
GCATTGGCTACTCTTGTTAT+AGG 0.258949 tig0019197:-130940 Msa1371380:CDS
CTTAATGTTGCTCGAGCTAT+TGG 0.303340 tig0019197:-130494 Msa1371380:CDS
GCAATAGATGGATTAGGATT+TGG 0.325019 tig0019197:+132362 None:intergenic
CATAGGCTGCGATGGGATTT+GGG 0.327999 tig0019197:-130369 Msa1371380:CDS
GATGGACCGAGTGCTTTCTA+TGG 0.328546 tig0019197:-131543 Msa1371380:CDS
TATCGGGTTCCGGTCAAATA+TGG 0.330817 tig0019197:-131628 Msa1371380:CDS
AGTTTCTGACTGTAGGTTGC+TGG 0.335395 tig0019197:-130669 Msa1371380:intron
TCCGGAGGATGTGCTGATAT+CGG 0.336894 tig0019197:-131645 Msa1371380:CDS
GAGCGTGGTGCTACCAAATT+TGG 0.350164 tig0019197:+130185 None:intergenic
CTCAATAAACAACTTATAGA+TGG 0.363410 tig0019197:-131561 Msa1371380:CDS
GTCTATGATGGATATCTTAA+CGG 0.377116 tig0019197:-130521 Msa1371380:CDS
CAGAGGCGTATCGAGGCATC+TGG 0.390804 tig0019197:-130551 Msa1371380:CDS
TGTTACTTGTTAGGTGTAGT+AGG 0.391570 tig0019197:-129974 Msa1371380:three_prime_UTR
CATCCTCCGGAACGGAGCTT+TGG 0.398949 tig0019197:+131657 None:intergenic
TCGGTGGTCGGTGAGGGTTT+GGG 0.400048 tig0019197:+132690 None:intergenic
CTTTCATGCAACCTGGTTTG+TGG 0.400398 tig0019197:+130576 None:intergenic
TTCCTTCCAAAGCTCCGTTC+CGG 0.404057 tig0019197:-131663 Msa1371380:CDS
CTCTGCTCTTTCATGCAACC+TGG 0.407237 tig0019197:+130569 None:intergenic
AGTTACCAAAATTTGAAAAT+GGG 0.411806 tig0019197:+132489 None:intergenic
AACTGTAGGTGTTACTTGTT+AGG 0.413137 tig0019197:-129983 Msa1371380:intron
AAAAGAGTGTGTTGGGAAAG+AGG 0.414423 tig0019197:+132637 None:intergenic
ATGTGGAGATTTGTTTGAAA+GGG 0.417255 tig0019197:+132607 None:intergenic
GCAGAAGCAATAGATGGATT+AGG 0.417848 tig0019197:+132356 None:intergenic
TGAATCTCAATTCTCATAAG+AGG 0.424835 tig0019197:+130040 None:intergenic
CGGTGGTCGGTGGTCGGTGA+GGG 0.425084 tig0019197:+132684 None:intergenic
CGAAAAGCAGAAGCAATAGA+TGG 0.432400 tig0019197:+132350 None:intergenic
TGACTGTAGGTTGCTGGTGG+TGG 0.438915 tig0019197:-130663 Msa1371380:intron
TGGCTTGGTCAGGTGTTTGA+TGG 0.441121 tig0019197:-131138 Msa1371380:intron
CCGGAGGATGTGCTGATATC+GGG 0.442854 tig0019197:-131644 Msa1371380:CDS
AGAGGCGTATCGAGGCATCT+GGG 0.450237 tig0019197:-130550 Msa1371380:CDS
CTCAATGCTGCCCTTGCATC+CGG 0.452615 tig0019197:-130970 Msa1371380:CDS
TTGCTGGTGGTGGCAAATGC+TGG 0.454788 tig0019197:-130653 Msa1371380:CDS
ATGCAGTGGTGCCGGATGCA+AGG 0.459353 tig0019197:+130959 None:intergenic
GCAAACAGACTATGCATTTG+CGG 0.463349 tig0019197:-131004 Msa1371380:CDS
TTTGAAGAAAAGAGTGTGTT+GGG 0.473612 tig0019197:+132630 None:intergenic
AATGTGGAGATTTGTTTGAA+AGG 0.474500 tig0019197:+132606 None:intergenic
TGCATCCGGCACCACTGCAT+TGG 0.476188 tig0019197:-130956 Msa1371380:CDS
TTTATATGTCGGCGATTCAA+AGG 0.478964 tig0019197:+132572 None:intergenic
TCTATGATGGATATCTTAAC+GGG 0.479521 tig0019197:-130520 Msa1371380:CDS
TGCAGTGGTGCCGGATGCAA+GGG 0.482277 tig0019197:+130960 None:intergenic
AGCTGTATGCAGTAAGGATC+TGG 0.483557 tig0019197:-130282 Msa1371380:CDS
GATGTGCTGATATCGGGTTC+CGG 0.494574 tig0019197:-131638 Msa1371380:CDS
CCCGATATCAGCACATCCTC+CGG 0.497125 tig0019197:+131644 None:intergenic
AGATCCTTACTGCATACAGC+TGG 0.500453 tig0019197:+130284 None:intergenic
TAAGTTGTTTATTGAGCCCA+TGG 0.501821 tig0019197:+131568 None:intergenic
TTATCCTCGAGAATGAACTT+TGG 0.503539 tig0019197:+131072 None:intergenic
AGTAGCCAATGCAGTGGTGC+CGG 0.504279 tig0019197:+130951 None:intergenic
ACTATCCTTTCAATATCCAC+TGG 0.504474 tig0019197:+131042 None:intergenic
CACAGGAGCTTCTCTGCTGA+AGG 0.507072 tig0019197:-130155 Msa1371380:CDS
AGGCGTATCGAGGCATCTGG+GGG 0.513077 tig0019197:-130548 Msa1371380:CDS
ATGGACCGAGTGCTTTCTAT+GGG 0.513585 tig0019197:-131542 Msa1371380:CDS
GCGGTGGTCGGTGGTCGGTG+AGG 0.520865 tig0019197:+132683 None:intergenic
AGAATGACCGTCGTAAATGA+AGG 0.524484 tig0019197:+132661 None:intergenic
TTATATGTCGGCGATTCAAA+GGG 0.528456 tig0019197:+132573 None:intergenic
TTTCTCATCATAGGCTGCGA+TGG 0.528638 tig0019197:-130377 Msa1371380:CDS
TTCTCATCATAGGCTGCGAT+GGG 0.530891 tig0019197:-130376 Msa1371380:CDS
GAAGGAATGAAAGGCAAAGA+TGG 0.533012 tig0019197:-130458 Msa1371380:CDS
TGAGATTCATTTGTAACTGT+AGG 0.533426 tig0019197:-130026 Msa1371380:intron
TGCATAGTCTGTTTGCAGAA+AGG 0.536620 tig0019197:+131011 None:intergenic
AAAGGGTGGTTTAACAAATG+TGG 0.539391 tig0019197:+132590 None:intergenic
ATAGATGGATTAGGATTTGG+AGG 0.539565 tig0019197:+132365 None:intergenic
CGACCCAGCTGTATGCAGTA+AGG 0.541516 tig0019197:-130288 Msa1371380:CDS
TAAATGAAGGCGGTGGTCGG+TGG 0.544330 tig0019197:+132674 None:intergenic
GTTTGAAGAAAAGAGTGTGT+TGG 0.546377 tig0019197:+132629 None:intergenic
TAAAATAAAATTAAACAAGA+AGG 0.548575 tig0019197:+130067 None:intergenic
ATTTCCCAGTGGATATTGAA+AGG 0.550709 tig0019197:-131047 Msa1371380:CDS
CTTCCAAAGCTCCGTTCCGG+AGG 0.555450 tig0019197:-131660 Msa1371380:CDS
CGTGGTGCTACCAAATTTGG+TGG 0.556204 tig0019197:+130188 None:intergenic
TGGCTACTCTTGTTATAGGA+AGG 0.559140 tig0019197:-130936 Msa1371380:intron
GAGGCGTATCGAGGCATCTG+GGG 0.559861 tig0019197:-130549 Msa1371380:CDS
CTATCCTTTCAATATCCACT+GGG 0.563023 tig0019197:+131043 None:intergenic
ATGTCAAGAGACCACAAACC+AGG 0.567267 tig0019197:-130587 Msa1371380:CDS
AGAGAAGCTCCTGTGCACTC+TGG 0.567952 tig0019197:+130163 None:intergenic
TGGCACATGGAAGGAATGAA+AGG 0.568858 tig0019197:-130467 Msa1371380:CDS
GGTCAGGTGTTTGATGGACA+CGG 0.571598 tig0019197:-131132 Msa1371380:intron
TTTCTGCATGGTAAAACAAG+AGG 0.572653 tig0019197:+132308 None:intergenic
ATTGGTGACTGGCACATGGA+AGG 0.574252 tig0019197:-130476 Msa1371380:CDS
CTCCGGAACGGAGCTTTGGA+AGG 0.575225 tig0019197:+131661 None:intergenic
TTCTGACTGTAGGTTGCTGG+TGG 0.579507 tig0019197:-130666 Msa1371380:intron
TCGTAAATGAAGGCGGTGGT+CGG 0.582325 tig0019197:+132671 None:intergenic
TTGCTCGAGCTATTGGTGAC+TGG 0.582496 tig0019197:-130487 Msa1371380:CDS
GAGGACGAATTTCTCATCAT+AGG 0.583587 tig0019197:-130386 Msa1371380:CDS
CTCCTGTGCACTCTGGAGCG+TGG 0.585430 tig0019197:+130170 None:intergenic
ACACATCCTCCATATTTGAC+CGG 0.585526 tig0019197:+131619 None:intergenic
AGACAATTTAGCTGTTGTTG+TGG 0.586656 tig0019197:-130231 Msa1371380:CDS
TTTGCCAAAGTTCATTCTCG+AGG 0.590449 tig0019197:-131076 Msa1371380:CDS
TATGTCGGCGATTCAAAGGG+TGG 0.591649 tig0019197:+132576 None:intergenic
CAGTAAGGATCTGGTTGATG+AGG 0.591652 tig0019197:-130273 Msa1371380:CDS
TGAAGGCGGTGGTCGGTGGT+CGG 0.591726 tig0019197:+132678 None:intergenic
CGAGCAGTGCTGTGCCGTCG+TGG 0.598079 tig0019197:-130623 Msa1371380:CDS
GGGTTTGGGTTTCGAAGATG+CGG 0.598198 tig0019197:+132704 None:intergenic
AGCTATTGGTGACTGGCACA+TGG 0.600561 tig0019197:-130480 Msa1371380:CDS
CACCACGCTCCAGAGTGCAC+AGG 0.602591 tig0019197:-130172 Msa1371380:CDS
TTTGGGTTTCGAAGATGCGG+TGG 0.614214 tig0019197:+132707 None:intergenic
GCTTTGGAAGGAAATCAGAC+TGG 0.619255 tig0019197:+131673 None:intergenic
GGTGCTACCAAATTTGGTGG+AGG 0.621044 tig0019197:+130191 None:intergenic
CGGGTTCCGGTCAAATATGG+AGG 0.621074 tig0019197:-131625 Msa1371380:CDS
GGAATGAAAGGCAAAGATGG+TGG 0.624005 tig0019197:-130455 Msa1371380:CDS
AGTTCAGGCTCTGCACTGAG+TGG 0.625933 tig0019197:+130431 None:intergenic
GACTACAAAACTTACTGCCG+AGG 0.627191 tig0019197:-130405 Msa1371380:CDS
TATGATGAGAAATTCGTCCT+CGG 0.637476 tig0019197:+130388 None:intergenic
GATCCTTACTGCATACAGCT+GGG 0.642529 tig0019197:+130285 None:intergenic
GGTGTGAATTGTTTCAGTGA+AGG 0.642841 tig0019197:-132410 Msa1371380:CDS
ACCGTCGTAAATGAAGGCGG+TGG 0.644318 tig0019197:+132667 None:intergenic
TATCAGCACATCCTCCGGAA+CGG 0.646071 tig0019197:+131649 None:intergenic
GAAAGAGCAGAGGCGTATCG+AGG 0.646196 tig0019197:-130558 Msa1371380:CDS
TCTGTTGAGGATGTTACTGA+AGG 0.648815 tig0019197:-131699 Msa1371380:CDS
CTTTCTGACTGTATCATATG+CGG 0.655064 tig0019197:-129940 Msa1371380:three_prime_UTR
TGAAAACAAATCATAAAACA+AGG 0.659514 tig0019197:-132747 Msa1371380:five_prime_UTR
CAGGTTGCATGAAAGAGCAG+AGG 0.671998 tig0019197:-130568 Msa1371380:CDS
TATACCCCATAGAAAGCACT+CGG 0.672295 tig0019197:+131537 None:intergenic
AACAAGAGTAGCCAATGCAG+TGG 0.673761 tig0019197:+130945 None:intergenic
GTTGTCTGATGCATCTGTTG+AGG 0.696072 tig0019197:-131712 Msa1371380:CDS
GGCGTATCGAGGCATCTGGG+GGG 0.697326 tig0019197:-130547 Msa1371380:CDS
ACCACCGCCTTCATTTACGA+CGG 0.704422 tig0019197:-132668 Msa1371380:five_prime_UTR
GGTTTCTTACAAGAGAAGCG+TGG 0.710252 tig0019197:+132273 None:intergenic
CGAGGATAAAGATTTCCCAG+TGG 0.737162 tig0019197:-131058 Msa1371380:CDS
TGGACCGAGTGCTTTCTATG+GGG 0.737710 tig0019197:-131541 Msa1371380:intron
ATGACCGTCGTAAATGAAGG+CGG 0.742021 tig0019197:+132664 None:intergenic
CAGGTGTTTGATGGACACGG+CGG 0.806389 tig0019197:-131129 Msa1371380:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTGATATTCAATAATTT+GGG - tig0019197:131750-131769 Msa1371380:intron 10.0%
!! TAAAATAAAATTAAACAAGA+AGG + tig0019197:132594-132613 None:intergenic 10.0%
!! ATTTAATGTAGATAAAAACT+AGG - tig0019197:130617-130636 Msa1371380:CDS 15.0%
!!! GATTTTGATATTCAATAATT+TGG - tig0019197:131749-131768 Msa1371380:intron 15.0%
!! GCTAATTTATAGTAAAAAAA+AGG + tig0019197:131783-131802 None:intergenic 15.0%
!!! TTTATGATTTGTTTTCACAA+CGG + tig0019197:129908-129927 None:intergenic 20.0%
!! TGAAAACAAATCATAAAACA+AGG - tig0019197:129911-129930 Msa1371380:three_prime_UTR 20.0%
!!! ATTAGGTCTTTTTTATATGT+CGG + tig0019197:130100-130119 None:intergenic 20.0%
!!! TCAGAAAAAGTGTAAAAATT+AGG + tig0019197:130117-130136 None:intergenic 20.0%
!!! TTAAACCCATTTTCAAATTT+TGG - tig0019197:130164-130183 Msa1371380:CDS 20.0%
!! AGTTACCAAAATTTGAAAAT+GGG + tig0019197:130172-130191 None:intergenic 20.0%
!!! ATTTTCAAATTTTGGTAACT+GGG - tig0019197:130172-130191 Msa1371380:CDS 20.0%
!! CAAAAGAAAAAACAAAAGAT+CGG + tig0019197:130409-130428 None:intergenic 20.0%
!!! ATAATGATGTTACTTAGTTT+TGG - tig0019197:130457-130476 Msa1371380:CDS 20.0%
!! GAAATGAAATGAAATAATGA+TGG - tig0019197:130673-130692 Msa1371380:intron 20.0%
!!! TAATTGTTGATTATGTTTTG+TGG - tig0019197:130755-130774 Msa1371380:intron 20.0%
!! ATAATCAATAATCAATCCTA+AGG + tig0019197:130794-130813 None:intergenic 20.0%
!!! TAGGATTGATTATTGATTAT+TGG - tig0019197:130794-130813 Msa1371380:intron 20.0%
!! TGATATGATAGTTGTTAAAA+TGG - tig0019197:131175-131194 Msa1371380:intron 20.0%
!! GATATGATAGTTGTTAAAAT+GGG - tig0019197:131176-131195 Msa1371380:intron 20.0%
!!! TGCTAATCTTATTTTGTAAT+TGG - tig0019197:131332-131351 Msa1371380:intron 20.0%
!!! TTTTTTTTTGAACAAACTGT+AGG - tig0019197:132661-132680 Msa1371380:five_prime_UTR 20.0%
! CAGTTACCAAAATTTGAAAA+TGG + tig0019197:130173-130192 None:intergenic 25.0%
!!! CATTTTCAAATTTTGGTAAC+TGG - tig0019197:130171-130190 Msa1371380:CDS 25.0%
!!! TTTTTGAAGTTTGGATCTTA+TGG - tig0019197:130201-130220 Msa1371380:CDS 25.0%
! GATCTTATGGTTAAGAAAAA+TGG - tig0019197:130214-130233 Msa1371380:CDS 25.0%
! AGAAAAATGGAAGAAATTGA+AGG - tig0019197:130227-130246 Msa1371380:CDS 25.0%
! TGACAAATGGAAAGAAAAAA+AGG + tig0019197:130555-130574 None:intergenic 25.0%
! AGAATGTGTAATTTAAGCTT+TGG - tig0019197:130588-130607 Msa1371380:CDS 25.0%
! ATGAAATGAAATAATGATGG+CGG - tig0019197:130676-130695 Msa1371380:intron 25.0%
!!! TATTGATTATTGGTGTTAGT+AGG - tig0019197:130804-130823 Msa1371380:intron 25.0%
!!! ATTGATTATTGGTGTTAGTA+GGG - tig0019197:130805-130824 Msa1371380:intron 25.0%
!!! ATGTTTGTGTTGACAATTTT+AGG - tig0019197:131062-131081 Msa1371380:CDS 25.0%
! CTCAATAAACAACTTATAGA+TGG - tig0019197:131097-131116 Msa1371380:CDS 25.0%
! TACAAAATAAGATTAGCATG+TGG + tig0019197:131330-131349 None:intergenic 25.0%
! TAATATGTGTTTATGCACAT+TGG + tig0019197:131381-131400 None:intergenic 25.0%
! TTACTATAAATTAGCACCAA+AGG - tig0019197:131788-131807 Msa1371380:intron 25.0%
!!! CATCTGACATTTTAATTTAC+TGG - tig0019197:132741-132760 Msa1371380:five_prime_UTR 25.0%
! TTTGAAGAAAAGAGTGTGTT+GGG + tig0019197:130031-130050 None:intergenic 30.0%
! ATGTGGAGATTTGTTTGAAA+GGG + tig0019197:130054-130073 None:intergenic 30.0%
! AATGTGGAGATTTGTTTGAA+AGG + tig0019197:130055-130074 None:intergenic 30.0%
CCAGATTGTAAAAAGCAATT+GGG + tig0019197:130143-130162 None:intergenic 30.0%
!!! CCAATTGCTTTTTACAATCT+GGG - tig0019197:130141-130160 Msa1371380:CDS 30.0%
AAAACTAGGACTAGAATAGA+AGG - tig0019197:130631-130650 Msa1371380:CDS 30.0%
TGAAATGAAATAATGATGGC+GGG - tig0019197:130677-130696 Msa1371380:intron 30.0%
AAAAGAGAATAACGACACAT+GGG + tig0019197:131230-131249 None:intergenic 30.0%
AATGAAAGAAGACAAAACGA+AGG + tig0019197:131253-131272 None:intergenic 30.0%
AAAAACTCCATAGCTAGTAA+AGG + tig0019197:131410-131429 None:intergenic 30.0%
CAGTTGACAAGAATAGATAT+AGG - tig0019197:131431-131450 Msa1371380:intron 30.0%
AAGTAACGTTATATGTCGAA+TGG - tig0019197:131500-131519 Msa1371380:intron 30.0%
CAAGAAATTTAGCATACCTT+TGG + tig0019197:131807-131826 None:intergenic 30.0%
AAAAGACATAAATCTGACTG+AGG - tig0019197:131849-131868 Msa1371380:intron 30.0%
GAGCATGAAAATGGAAATAT+GGG + tig0019197:131908-131927 None:intergenic 30.0%
AGAGCATGAAAATGGAAATA+TGG + tig0019197:131909-131928 None:intergenic 30.0%
!! GTCTATGATGGATATCTTAA+CGG - tig0019197:132137-132156 Msa1371380:intron 30.0%
!! TCTATGATGGATATCTTAAC+GGG - tig0019197:132138-132157 Msa1371380:intron 30.0%
TGAATCTCAATTCTCATAAG+AGG + tig0019197:132621-132640 None:intergenic 30.0%
TGAGATTCATTTGTAACTGT+AGG - tig0019197:132632-132651 Msa1371380:five_prime_UTR 30.0%
! GTTTGAAGAAAAGAGTGTGT+TGG + tig0019197:130032-130051 None:intergenic 35.0%
AAAGGGTGGTTTAACAAATG+TGG + tig0019197:130071-130090 None:intergenic 35.0%
TTATATGTCGGCGATTCAAA+GGG + tig0019197:130088-130107 None:intergenic 35.0%
TTTATATGTCGGCGATTCAA+AGG + tig0019197:130089-130108 None:intergenic 35.0%
!!! CCCAATTGCTTTTTACAATC+TGG - tig0019197:130140-130159 Msa1371380:CDS 35.0%
CCCAGATTGTAAAAAGCAAT+TGG + tig0019197:130144-130163 None:intergenic 35.0%
!! ATAGATGGATTAGGATTTGG+AGG + tig0019197:130296-130315 None:intergenic 35.0%
!! GCAATAGATGGATTAGGATT+TGG + tig0019197:130299-130318 None:intergenic 35.0%
TTTCTGCATGGTAAAACAAG+AGG + tig0019197:130353-130372 None:intergenic 35.0%
CACTACATGAAAGTGACAAA+TGG + tig0019197:130568-130587 None:intergenic 35.0%
!! TTGTGTTGACAATTTTAGGC+AGG - tig0019197:131066-131085 Msa1371380:CDS 35.0%
!! TGTGTTGACAATTTTAGGCA+GGG - tig0019197:131067-131086 Msa1371380:CDS 35.0%
TAAGTTGTTTATTGAGCCCA+TGG + tig0019197:131093-131112 None:intergenic 35.0%
AGTACTCCAATCAAGATAAG+CGG + tig0019197:131156-131175 None:intergenic 35.0%
AAAGAGAATAACGACACATG+GGG + tig0019197:131229-131248 None:intergenic 35.0%
GAAAAGAGAATAACGACACA+TGG + tig0019197:131231-131250 None:intergenic 35.0%
AATTGGTACTGCTTTACCTT+TGG - tig0019197:131349-131368 Msa1371380:intron 35.0%
TTATCCTCGAGAATGAACTT+TGG + tig0019197:131589-131608 None:intergenic 35.0%
ATTTCCCAGTGGATATTGAA+AGG - tig0019197:131611-131630 Msa1371380:CDS 35.0%
CTATCCTTTCAATATCCACT+GGG + tig0019197:131618-131637 None:intergenic 35.0%
ACTATCCTTTCAATATCCAC+TGG + tig0019197:131619-131638 None:intergenic 35.0%
!!! AATTTCTTGTCCGCGTTTTT+TGG - tig0019197:131818-131837 Msa1371380:intron 35.0%
TTGTACTTGTCCAAAAAACG+CGG + tig0019197:131831-131850 None:intergenic 35.0%
ACATAAATCTGACTGAGGTT+TGG - tig0019197:131854-131873 Msa1371380:intron 35.0%
CATAAATCTGACTGAGGTTT+GGG - tig0019197:131855-131874 Msa1371380:intron 35.0%
! TTTTGGTCTGCTACAAAAGT+TGG - tig0019197:131882-131901 Msa1371380:intron 35.0%
TGTATTTGATGTCATGTCAG+AGG + tig0019197:131933-131952 None:intergenic 35.0%
TGATTGTAGTTTCTGACTGT+AGG - tig0019197:131982-132001 Msa1371380:intron 35.0%
!! AGTTTTGTAGTCATGAGTTC+AGG + tig0019197:132245-132264 None:intergenic 35.0%
TATGATGAGAAATTCGTCCT+CGG + tig0019197:132273-132292 None:intergenic 35.0%
! AAAATGCTGTAGATTTTGCG+CGG - tig0019197:132327-132346 Msa1371380:CDS 35.0%
AGACAATTTAGCTGTTGTTG+TGG - tig0019197:132427-132446 Msa1371380:CDS 35.0%
! GAAAGAGTTGCAAAGCTTTT+TGG - tig0019197:132529-132548 Msa1371380:five_prime_UTR 35.0%
! CAAAGCTTTTTGGATAGCTT+GGG - tig0019197:132539-132558 Msa1371380:five_prime_UTR 35.0%
TGAGTTTGCGCAAGTATTTA+TGG - tig0019197:132566-132585 Msa1371380:five_prime_UTR 35.0%
!! AACTGTAGGTGTTACTTGTT+AGG - tig0019197:132675-132694 Msa1371380:five_prime_UTR 35.0%
! TGTTACTTGTTAGGTGTAGT+AGG - tig0019197:132684-132703 Msa1371380:five_prime_UTR 35.0%
CTTTCTGACTGTATCATATG+CGG - tig0019197:132718-132737 Msa1371380:five_prime_UTR 35.0%
AGAATGACCGTCGTAAATGA+AGG + tig0019197:130000-130019 None:intergenic 40.0%
! AAAAGAGTGTGTTGGGAAAG+AGG + tig0019197:130024-130043 None:intergenic 40.0%
!!! GGGTCTGTGTTTTTGAAGTT+TGG - tig0019197:130192-130211 Msa1371380:CDS 40.0%
GGTGTGAATTGTTTCAGTGA+AGG - tig0019197:130248-130267 Msa1371380:CDS 40.0%
! GCAGAAGCAATAGATGGATT+AGG + tig0019197:130305-130324 None:intergenic 40.0%
CGAAAAGCAGAAGCAATAGA+TGG + tig0019197:130311-130330 None:intergenic 40.0%
! CGAACAAGAGATTTTCTGCA+TGG + tig0019197:130365-130384 None:intergenic 40.0%
!!! GAAATAATGATGGCGGGTTT+TGG - tig0019197:130683-130702 Msa1371380:intron 40.0%
!!! GGTTTTGGTTTGGTCAGATT+GGG - tig0019197:130698-130717 Msa1371380:intron 40.0%
!!! GTTTTGGTTTGGTCAGATTG+GGG - tig0019197:130699-130718 Msa1371380:intron 40.0%
TGGATTCAGTTTGCAACCTT+AGG - tig0019197:130775-130794 Msa1371380:intron 40.0%
! GGTGTTAGTAGGGTTATTTC+TGG - tig0019197:130815-130834 Msa1371380:intron 40.0%
TCTGTTGAGGATGTTACTGA+AGG - tig0019197:130959-130978 Msa1371380:CDS 40.0%
ACACATCCTCCATATTTGAC+CGG + tig0019197:131042-131061 None:intergenic 40.0%
!! TATACCCCATAGAAAGCACT+CGG + tig0019197:131124-131143 None:intergenic 40.0%
GAACTTCCAAAAGATGCCAT+CGG + tig0019197:131292-131311 None:intergenic 40.0%
ACGTTATATGTCGAATGGCT+TGG - tig0019197:131505-131524 Msa1371380:intron 40.0%
TTTGCCAAAGTTCATTCTCG+AGG - tig0019197:131582-131601 Msa1371380:CDS 40.0%
TGCATAGTCTGTTTGCAGAA+AGG + tig0019197:131650-131669 None:intergenic 40.0%
GCAAACAGACTATGCATTTG+CGG - tig0019197:131654-131673 Msa1371380:CDS 40.0%
!! GCATTGGCTACTCTTGTTAT+AGG - tig0019197:131718-131737 Msa1371380:CDS 40.0%
! TGGCTACTCTTGTTATAGGA+AGG - tig0019197:131722-131741 Msa1371380:CDS 40.0%
! ACTCTTGTTATAGGAAGGTC+AGG - tig0019197:131727-131746 Msa1371380:CDS 40.0%
!! ACTGAGGTTTGGGATCTTTT+TGG - tig0019197:131865-131884 Msa1371380:intron 40.0%
! TCAGAGGAAGAGCATGAAAA+TGG + tig0019197:131917-131936 None:intergenic 40.0%
CTTAATGTTGCTCGAGCTAT+TGG - tig0019197:132164-132183 Msa1371380:intron 40.0%
GAAGGAATGAAAGGCAAAGA+TGG - tig0019197:132200-132219 Msa1371380:intron 40.0%
GAGGACGAATTTCTCATCAT+AGG - tig0019197:132272-132291 Msa1371380:intron 40.0%
! GCGCAAAATCTACAGCATTT+TGG + tig0019197:132328-132347 None:intergenic 40.0%
!! GAGGCTTTGAAGAGAAAAAG+TGG - tig0019197:132404-132423 Msa1371380:CDS 40.0%
! GCAAAGCTTTTTGGATAGCT+TGG - tig0019197:132538-132557 Msa1371380:five_prime_UTR 40.0%
ATGACCGTCGTAAATGAAGG+CGG + tig0019197:129997-130016 None:intergenic 45.0%
GGTTTCTTACAAGAGAAGCG+TGG + tig0019197:130388-130407 None:intergenic 45.0%
!!! AATGATGGCGGGTTTTGGTT+TGG - tig0019197:130688-130707 Msa1371380:intron 45.0%
!!! GGGTTTTGGTTTGGTCAGAT+TGG - tig0019197:130697-130716 Msa1371380:intron 45.0%
GTTGTCTGATGCATCTGTTG+AGG - tig0019197:130946-130965 Msa1371380:CDS 45.0%
!! GCTTTGGAAGGAAATCAGAC+TGG + tig0019197:130988-131007 None:intergenic 45.0%
TATCGGGTTCCGGTCAAATA+TGG - tig0019197:131030-131049 Msa1371380:CDS 45.0%
! ACAATTTTAGGCAGGGCCAT+GGG - tig0019197:131074-131093 Msa1371380:CDS 45.0%
ATGGACCGAGTGCTTTCTAT+GGG - tig0019197:131116-131135 Msa1371380:CDS 45.0%
CTCTCTCCGCTTATCTTGAT+TGG - tig0019197:131147-131166 Msa1371380:intron 45.0%
GTACTGCTTTACCTTTGGTG+TGG - tig0019197:131354-131373 Msa1371380:intron 45.0%
!! ATATGTCGAATGGCTTGGTC+AGG - tig0019197:131510-131529 Msa1371380:intron 45.0%
CGAGGATAAAGATTTCCCAG+TGG - tig0019197:131600-131619 Msa1371380:CDS 45.0%
AACAAGAGTAGCCAATGCAG+TGG + tig0019197:131716-131735 None:intergenic 45.0%
AGTTTCTGACTGTAGGTTGC+TGG - tig0019197:131989-132008 Msa1371380:intron 45.0%
!! TGTCGATTGCTTTTCCACGA+CGG + tig0019197:132052-132071 None:intergenic 45.0%
ATGTCAAGAGACCACAAACC+AGG - tig0019197:132071-132090 Msa1371380:intron 45.0%
! CTTTCATGCAACCTGGTTTG+TGG + tig0019197:132085-132104 None:intergenic 45.0%
TGGCACATGGAAGGAATGAA+AGG - tig0019197:132191-132210 Msa1371380:intron 45.0%
GGAATGAAAGGCAAAGATGG+TGG - tig0019197:132203-132222 Msa1371380:intron 45.0%
GACTACAAAACTTACTGCCG+AGG - tig0019197:132253-132272 Msa1371380:intron 45.0%
TTTCTCATCATAGGCTGCGA+TGG - tig0019197:132281-132300 Msa1371380:intron 45.0%
TTCTCATCATAGGCTGCGAT+GGG - tig0019197:132282-132301 Msa1371380:intron 45.0%
GATCCTTACTGCATACAGCT+GGG + tig0019197:132376-132395 None:intergenic 45.0%
AGATCCTTACTGCATACAGC+TGG + tig0019197:132377-132396 None:intergenic 45.0%
AGCTGTATGCAGTAAGGATC+TGG - tig0019197:132376-132395 Msa1371380:CDS 45.0%
! CAGTAAGGATCTGGTTGATG+AGG - tig0019197:132385-132404 Msa1371380:CDS 45.0%
! TTTGGGTTTCGAAGATGCGG+TGG + tig0019197:129954-129973 None:intergenic 50.0%
! GGGTTTGGGTTTCGAAGATG+CGG + tig0019197:129957-129976 None:intergenic 50.0%
TCGTAAATGAAGGCGGTGGT+CGG + tig0019197:129990-130009 None:intergenic 50.0%
ACCACCGCCTTCATTTACGA+CGG - tig0019197:129990-130009 Msa1371380:intron 50.0%
TATGTCGGCGATTCAAAGGG+TGG + tig0019197:130085-130104 None:intergenic 50.0%
TTCCTTCCAAAGCTCCGTTC+CGG - tig0019197:130995-131014 Msa1371380:CDS 50.0%
TATCAGCACATCCTCCGGAA+CGG + tig0019197:131012-131031 None:intergenic 50.0%
! TCCGGAGGATGTGCTGATAT+CGG - tig0019197:131013-131032 Msa1371380:CDS 50.0%
! GATGTGCTGATATCGGGTTC+CGG - tig0019197:131020-131039 Msa1371380:CDS 50.0%
! GACAATTTTAGGCAGGGCCA+TGG - tig0019197:131073-131092 Msa1371380:CDS 50.0%
GATGGACCGAGTGCTTTCTA+TGG - tig0019197:131115-131134 Msa1371380:CDS 50.0%
TGGACCGAGTGCTTTCTATG+GGG - tig0019197:131117-131136 Msa1371380:CDS 50.0%
! GCACATCCGATGGCATCTTT+TGG - tig0019197:131283-131302 Msa1371380:intron 50.0%
TGCACATTGGTCCACACCAA+AGG + tig0019197:131368-131387 None:intergenic 50.0%
AGACGCGCCTTTACTAGCTA+TGG - tig0019197:131400-131419 Msa1371380:intron 50.0%
!! TGGCTTGGTCAGGTGTTTGA+TGG - tig0019197:131520-131539 Msa1371380:intron 50.0%
! GGTCAGGTGTTTGATGGACA+CGG - tig0019197:131526-131545 Msa1371380:intron 50.0%
TTCTGACTGTAGGTTGCTGG+TGG - tig0019197:131992-132011 Msa1371380:intron 50.0%
CTCTGCTCTTTCATGCAACC+TGG + tig0019197:132092-132111 None:intergenic 50.0%
CAGGTTGCATGAAAGAGCAG+AGG - tig0019197:132090-132109 Msa1371380:intron 50.0%
! TTGCTCGAGCTATTGGTGAC+TGG - tig0019197:132171-132190 Msa1371380:intron 50.0%
! AGCTATTGGTGACTGGCACA+TGG - tig0019197:132178-132197 Msa1371380:intron 50.0%
! ATTGGTGACTGGCACATGGA+AGG - tig0019197:132182-132201 Msa1371380:intron 50.0%
TCATAGGCTGCGATGGGATT+TGG - tig0019197:132288-132307 Msa1371380:CDS 50.0%
CATAGGCTGCGATGGGATTT+GGG - tig0019197:132289-132308 Msa1371380:CDS 50.0%
! GGTGCTACCAAATTTGGTGG+AGG + tig0019197:132470-132489 None:intergenic 50.0%
!! CGTGGTGCTACCAAATTTGG+TGG + tig0019197:132473-132492 None:intergenic 50.0%
!! GAGCGTGGTGCTACCAAATT+TGG + tig0019197:132476-132495 None:intergenic 50.0%
TAAATGAAGGCGGTGGTCGG+TGG + tig0019197:129987-130006 None:intergenic 55.0%
ACCGTCGTAAATGAAGGCGG+TGG + tig0019197:129994-130013 None:intergenic 55.0%
CCCGATATCAGCACATCCTC+CGG + tig0019197:131017-131036 None:intergenic 55.0%
! CCGGAGGATGTGCTGATATC+GGG - tig0019197:131014-131033 Msa1371380:CDS 55.0%
CGGGTTCCGGTCAAATATGG+AGG - tig0019197:131033-131052 Msa1371380:CDS 55.0%
CTCCTGTTCTGCACATCCGA+TGG - tig0019197:131273-131292 Msa1371380:intron 55.0%
TGCCATCGGATGTGCAGAAC+AGG + tig0019197:131278-131297 None:intergenic 55.0%
! CAGGTGTTTGATGGACACGG+CGG - tig0019197:131529-131548 Msa1371380:intron 55.0%
CTCAATGCTGCCCTTGCATC+CGG - tig0019197:131688-131707 Msa1371380:CDS 55.0%
AGTAGCCAATGCAGTGGTGC+CGG + tig0019197:131710-131729 None:intergenic 55.0%
TGACTGTAGGTTGCTGGTGG+TGG - tig0019197:131995-132014 Msa1371380:intron 55.0%
!! TTGCTGGTGGTGGCAAATGC+TGG - tig0019197:132005-132024 Msa1371380:intron 55.0%
! GAAAGAGCAGAGGCGTATCG+AGG - tig0019197:132100-132119 Msa1371380:intron 55.0%
! AGAGGCGTATCGAGGCATCT+GGG - tig0019197:132108-132127 Msa1371380:intron 55.0%
TCTGGGGGGTCTGTCTATGA+TGG - tig0019197:132125-132144 Msa1371380:intron 55.0%
AGTTCAGGCTCTGCACTGAG+TGG + tig0019197:132230-132249 None:intergenic 55.0%
CGACCCAGCTGTATGCAGTA+AGG - tig0019197:132370-132389 Msa1371380:CDS 55.0%
GCAGCAGCCTCCACCAAATT+TGG - tig0019197:132460-132479 Msa1371380:five_prime_UTR 55.0%
AGAGAAGCTCCTGTGCACTC+TGG + tig0019197:132498-132517 None:intergenic 55.0%
CACAGGAGCTTCTCTGCTGA+AGG - tig0019197:132503-132522 Msa1371380:five_prime_UTR 55.0%
TCGGTGGTCGGTGAGGGTTT+GGG + tig0019197:129971-129990 None:intergenic 60.0%
!! CTCCGGAACGGAGCTTTGGA+AGG + tig0019197:131000-131019 None:intergenic 60.0%
CTTCCAAAGCTCCGTTCCGG+AGG - tig0019197:130998-131017 Msa1371380:CDS 60.0%
CATCCTCCGGAACGGAGCTT+TGG + tig0019197:131004-131023 None:intergenic 60.0%
! TGCAGTGGTGCCGGATGCAA+GGG + tig0019197:131701-131720 None:intergenic 60.0%
! ATGCAGTGGTGCCGGATGCA+AGG + tig0019197:131702-131721 None:intergenic 60.0%
TGCATCCGGCACCACTGCAT+TGG - tig0019197:131702-131721 Msa1371380:CDS 60.0%
! CAGAGGCGTATCGAGGCATC+TGG - tig0019197:132107-132126 Msa1371380:intron 60.0%
! GAGGCGTATCGAGGCATCTG+GGG - tig0019197:132109-132128 Msa1371380:intron 60.0%
! AGGCGTATCGAGGCATCTGG+GGG - tig0019197:132110-132129 Msa1371380:intron 60.0%
! CTGTAGATTTTGCGCGGCGC+AGG - tig0019197:132333-132352 Msa1371380:CDS 60.0%
GTCGGTGGTCGGTGAGGGTT+TGG + tig0019197:129972-129991 None:intergenic 65.0%
TGAAGGCGGTGGTCGGTGGT+CGG + tig0019197:129983-130002 None:intergenic 65.0%
! GGCGTATCGAGGCATCTGGG+GGG - tig0019197:132111-132130 Msa1371380:intron 65.0%
! TTTTGCGCGGCGCAGGCTTC+AGG - tig0019197:132340-132359 Msa1371380:CDS 65.0%
CACCACGCTCCAGAGTGCAC+AGG - tig0019197:132486-132505 Msa1371380:five_prime_UTR 65.0%
!! CTCCTGTGCACTCTGGAGCG+TGG + tig0019197:132491-132510 None:intergenic 65.0%
CGGTGGTCGGTGGTCGGTGA+GGG + tig0019197:129977-129996 None:intergenic 70.0%
CGAGCAGTGCTGTGCCGTCG+TGG - tig0019197:132035-132054 Msa1371380:intron 70.0%
GCGGTGGTCGGTGGTCGGTG+AGG + tig0019197:129978-129997 None:intergenic 75.0%
Chromosome Type Strat End Strand Name
tig0019197 gene 129890 132790 129890 ID=Msa1371380;Name=Msa1371380
tig0019197 mRNA 129890 132790 129890 ID=Msa1371380-mRNA-1;Parent=Msa1371380;Name=Msa1371380-mRNA-1;_AED=0.12;_eAED=0.12;_QI=343|1|1|1|0.75|0.6|5|193|382
tig0019197 exon 129890 129997 129890 ID=Msa1371380-mRNA-1:exon:2261;Parent=Msa1371380-mRNA-1
tig0019197 exon 130027 130676 130027 ID=Msa1371380-mRNA-1:exon:2260;Parent=Msa1371380-mRNA-1
tig0019197 exon 130937 131148 130937 ID=Msa1371380-mRNA-1:exon:2259;Parent=Msa1371380-mRNA-1
tig0019197 exon 131542 131748 131542 ID=Msa1371380-mRNA-1:exon:2258;Parent=Msa1371380-mRNA-1
tig0019197 exon 132283 132790 132283 ID=Msa1371380-mRNA-1:exon:2257;Parent=Msa1371380-mRNA-1
tig0019197 five_prime_UTR 132448 132790 132448 ID=Msa1371380-mRNA-1:five_prime_utr;Parent=Msa1371380-mRNA-1
tig0019197 CDS 132283 132447 132283 ID=Msa1371380-mRNA-1:cds;Parent=Msa1371380-mRNA-1
tig0019197 CDS 131542 131748 131542 ID=Msa1371380-mRNA-1:cds;Parent=Msa1371380-mRNA-1
tig0019197 CDS 130937 131148 130937 ID=Msa1371380-mRNA-1:cds;Parent=Msa1371380-mRNA-1
tig0019197 CDS 130112 130676 130112 ID=Msa1371380-mRNA-1:cds;Parent=Msa1371380-mRNA-1
tig0019197 three_prime_UTR 130027 130111 130027 ID=Msa1371380-mRNA-1:three_prime_utr;Parent=Msa1371380-mRNA-1
tig0019197 three_prime_UTR 129890 129997 129890 ID=Msa1371380-mRNA-1:three_prime_utr;Parent=Msa1371380-mRNA-1
Gene Sequence

>Msa1371380

ATGGAAGAAATTGAAGGTGTGAATTGTTTCAGTGAAGGTAGAAGCTCTAGTGAGAGTCGACCTCCAAATCCTAATCCATCTATTGCTTCTGCTTTTCGTCAATGTCAACGCAATGATCCTCTTGTTTTACCATGCAGAAAATCTCTTGTTCGCCACGCTTCTCTTCAGACGAAAATGAAGTTGTCTGATGCATCTGTTGAGGATGTTACTGAAGGTTGCCAGTCTGATTTCCTTCCAAAGCTCCGTTCCGGAGGATGTGCTGATATCGGGTTCCGGTCAAATATGGAGGATGTGTATGTTTGTGTTGACAATTTTAGGCAGGGCCATGGGCTCAATAAACAACTTATAGATGGACCGAGTGCTTTCTATGGGGTGTTTGATGGACACGGCGGAAAGCATGCTGCTGACTTTGCTTGCAATCATTTGCCAAAGTTCATTCTCGAGGATAAAGATTTCCCAGTGGATATTGAAAGGATAGTTGCTTCTGCCTTTCTGCAAACAGACTATGCATTTGCGGAAGCTTGTTCTCTCAATGCTGCCCTTGCATCCGGCACCACTGCATTGGCTACTCTTGTTATAGGAAGGTTGCTGGTGGTGGCAAATGCTGGAGATTGTCGAGCAGTGCTGTGCCGTCGTGGAAAAGCAATCGACATGTCAAGAGACCACAAACCAGGTTGCATGAAAGAGCAGAGGCGTATCGAGGCATCTGGGGGGTCTGTCTATGATGGATATCTTAACGGGCAACTTAATGTTGCTCGAGCTATTGGTGACTGGCACATGGAAGGAATGAAAGGCAAAGATGGTGGACCACTCAGTGCAGAGCCTGAACTCATGACTACAAAACTTACTGCCGAGGACGAATTTCTCATCATAGGCTGCGATGGGATTTGGGATGTTTTTAGAAGCCAAAATGCTGTAGATTTTGCGCGGCGCAGGCTTCAGGTGCATAACGACCCAGCTGTATGCAGTAAGGATCTGGTTGATGAGGCTTTGAAGAGAAAAAGTGGAGACAATTTAGCTGTTGTTGTGGTTTGCTTTCAGCAGCAGCCTCCACCAAATTTGGTAGCACCACGCTCCAGAGTGCACAGGAGCTTCTCTGCTGAAGGTTTGAAAGAGTTGCAAAGCTTTTTGGATAGCTTGGGAAAATGA
Protein sequence

>Msa1371380

MEEIEGVNCFSEGRSSSESRPPNPNPSIASAFRQCQRNDPLVLPCRKSLVRHASLQTKMKLSDASVEDVTEGCQSDFLPKLRSGGCADIGFRSNMEDVYVCVDNFRQGHGLNKQLIDGPSAFYGVFDGHGGKHAADFACNHLPKFILEDKDFPVDIERIVASAFLQTDYAFAEACSLNAALASGTTALATLVIGRLLVVANAGDCRAVLCRRGKAIDMSRDHKPGCMKEQRRIEASGGSVYDGYLNGQLNVARAIGDWHMEGMKGKDGGPLSAEPELMTTKLTAEDEFLIIGCDGIWDVFRSQNAVDFARRRLQVHNDPAVCSKDLVDEALKRKSGDNLAVVVVCFQQQPPPNLVAPRSRVHRSFSAEGLKELQSFLDSLGK*