AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1364260

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1364260 MtrunA17_Chr1g0208821 97.576 495 12 0 1 495 1 495 0.0 977
Msa1364260 MtrunA17_Chr1g0208881 58.714 482 190 1 8 489 1 473 0.0 577
Msa1364260 MtrunA17_Chr1g0208891 58.787 478 187 1 8 485 1 468 0.0 577
Msa1364260 MtrunA17_Chr7g0251331 55.368 475 206 3 23 492 4 477 0.0 535
Msa1364260 MtrunA17_Chr6g0449061 48.760 484 241 2 13 495 8 485 2.35e-167 481
Msa1364260 MtrunA17_Chr6g0483381 50.943 477 227 2 11 485 2 473 5.38e-166 478
Msa1364260 MtrunA17_Chr6g0483361 49.476 477 232 2 11 485 2 471 7.14e-162 467
Msa1364260 MtrunA17_Chr6g0477961 48.596 463 235 2 26 485 11 473 9.38e-151 439
Msa1364260 MtrunA17_Chr1g0208791 46.610 472 252 0 8 479 10 481 1.80e-147 431
Msa1364260 MtrunA17_Chr8g0392601 45.714 455 244 1 29 483 3 454 4.66e-135 399
Msa1364260 MtrunA17_Chr6g0483351 51.459 377 181 1 100 474 1 377 2.44e-132 390
Msa1364260 MtrunA17_Chr7g0251321 49.870 385 172 3 100 483 1 365 1.62e-127 376
Msa1364260 MtrunA17_Chr6g0483331 44.295 447 248 1 40 485 11 457 1.49e-124 371
Msa1364260 MtrunA17_Chr6g0483341 41.719 477 273 2 14 485 10 486 5.03e-124 371
Msa1364260 MtrunA17_Chr2g0317131 38.478 473 291 0 9 481 11 483 1.44e-108 331
Msa1364260 MtrunA17_Chr2g0317151 39.331 478 283 3 8 482 1 474 5.15e-108 329
Msa1364260 MtrunA17_Chr6g0483161 44.562 377 190 1 98 474 1 358 1.55e-105 319
Msa1364260 MtrunA17_Chr4g0024501 37.083 480 295 1 11 483 27 506 1.84e-105 324
Msa1364260 MtrunA17_Chr2g0317161 37.740 469 282 2 18 481 8 471 6.29e-104 319
Msa1364260 MtrunA17_Chr2g0322671 36.939 490 299 2 4 483 5 494 2.01e-102 316
Msa1364260 MtrunA17_Chr2g0317171 36.920 474 284 3 18 481 9 477 1.00e-98 306
Msa1364260 MtrunA17_Chr5g0428251 33.476 466 306 2 18 481 32 495 2.40e-89 284
Msa1364260 MtrunA17_Chr2g0317221 39.487 390 226 2 97 481 7 391 3.93e-89 278
Msa1364260 MtrunA17_Chr2g0288281 37.760 384 239 0 100 483 1 384 6.38e-86 270
Msa1364260 MtrunA17_Chr6g0467321 31.633 490 320 6 5 481 4 491 9.24e-86 273
Msa1364260 MtrunA17_Chr5g0412771 34.910 444 284 3 42 483 57 497 1.53e-83 267
Msa1364260 MtrunA17_Chr3g0140931 33.473 478 299 6 12 481 25 491 3.67e-83 267
Msa1364260 MtrunA17_Chr5g0397991 32.675 456 301 2 29 481 53 505 8.75e-83 266
Msa1364260 MtrunA17_Chr8g0368001 34.228 447 289 3 39 483 48 491 1.75e-81 262
Msa1364260 MtrunA17_Chr5g0442331 33.742 489 313 4 10 492 11 494 6.57e-81 260
Msa1364260 MtrunA17_Chr5g0412541 35.520 442 279 4 42 480 37 475 1.29e-80 259
Msa1364260 MtrunA17_Chr8g0389801 32.823 457 301 4 26 480 8 460 6.24e-79 254
Msa1364260 MtrunA17_Chr1g0203791 32.059 471 307 5 16 481 42 504 1.72e-78 255
Msa1364260 MtrunA17_Chr6g0473701 30.802 474 317 4 27 493 110 579 5.66e-76 250
Msa1364260 MtrunA17_Chr7g0254511 33.196 485 307 7 1 483 1 470 7.71e-76 246
Msa1364260 MtrunA17_Chr7g0254521 34.607 445 286 3 41 483 31 472 1.27e-74 243
Msa1364260 MtrunA17_Chr3g0132531 33.613 476 304 6 10 480 17 485 4.00e-74 242
Msa1364260 MtrunA17_Chr1g0154441 36.176 387 240 4 100 483 1 383 5.63e-71 231
Msa1364260 MtrunA17_Chr6g0468531 32.914 477 311 4 8 480 1 472 3.13e-68 226
Msa1364260 MtrunA17_Chr6g0468521 32.568 479 309 7 8 480 1 471 3.15e-68 226
Msa1364260 MtrunA17_Chr7g0254531 34.635 384 250 1 100 483 1 383 1.87e-65 216
Msa1364260 MtrunA17_Chr1g0202761 30.952 462 317 1 22 483 24 483 2.18e-63 214
Msa1364260 MtrunA17_Chr4g0013031 28.370 497 312 10 19 479 18 506 4.77e-62 211
Msa1364260 MtrunA17_Chr5g0412571 34.704 389 248 4 98 483 38 423 6.39e-62 208
Msa1364260 MtrunA17_Chr2g0295631 29.322 457 307 2 41 481 20 476 2.88e-61 208
Msa1364260 MtrunA17_Chr6g0460541 34.190 389 248 3 98 483 3 386 2.86e-60 203
Msa1364260 MtrunA17_Chr6g0468511 32.735 446 290 6 8 451 1 438 8.22e-60 204
Msa1364260 MtrunA17_Chr7g0248051 31.210 471 316 2 4 474 39 501 1.93e-56 196
Msa1364260 MtrunA17_Chr6g0483264 47.059 204 108 0 280 483 1 204 5.16e-53 178
Msa1364260 MtrunA17_Chr7g0254501 34.035 285 186 1 199 483 16 298 8.88e-51 175
Msa1364260 MtrunA17_Chr6g0483241 48.066 181 94 0 98 278 1 181 4.44e-49 166
Msa1364260 MtrunA17_Chr8g0364761 34.237 295 185 5 22 309 30 322 2.45e-45 161
Msa1364260 MtrunA17_Chr3g0132541 31.849 292 198 1 42 333 42 332 5.20e-40 149
Msa1364260 MtrunA17_Chr6g0487091 33.557 298 191 4 185 480 97 389 1.35e-39 148
Msa1364260 MtrunA17_Chr5g0440661 28.338 367 200 4 100 466 1 304 6.54e-37 138
Msa1364260 MtrunA17_Chr6g0483221 43.919 148 83 0 327 474 6 153 2.48e-35 129
Msa1364260 MtrunA17_Chr6g0460521 37.198 207 130 0 277 483 1 207 8.45e-33 124
Msa1364260 MtrunA17_Chr6g0483261 44.586 157 87 0 327 483 6 162 3.93e-32 121
Msa1364260 MtrunA17_Chr5g0398571 26.871 294 198 3 196 480 1 286 1.65e-27 112
Msa1364260 MtrunA17_Chr8g0364751 39.024 164 100 0 320 483 12 175 2.59e-27 108
Msa1364260 MtrunA17_Chr7g0215591 91.379 58 5 0 350 407 1 58 3.91e-26 101
Msa1364260 MtrunA17_Chr1g0208901 35.795 176 63 3 28 203 4 129 7.92e-24 96.7
Msa1364260 MtrunA17_Chr5g0442311 30.168 179 125 0 100 278 1 179 3.91e-21 90.9
Msa1364260 MtrunA17_Chr6g0460531 28.148 270 171 7 18 278 5 260 6.10e-21 92.4
Msa1364260 MtrunA17_Chr6g0483281 49.296 71 36 0 211 281 5 75 1.42e-13 66.2
Msa1364260 MtrunA17_Chr7g0254491 34.545 110 68 1 13 122 6 111 2.49e-12 63.5
Msa1364260 MtrunA17_Chr4g0025551 30.719 153 80 5 147 297 28 156 9.10e-12 63.2
Msa1364260 MtrunA17_Chr5g0398551 38.462 78 48 0 38 115 40 117 4.82e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1364260 AT1G15170.1 59.834 483 186 2 1 483 1 475 0.0 598
Msa1364260 AT1G15150.1 59.130 460 188 0 24 483 13 472 0.0 592
Msa1364260 AT1G15160.1 59.355 465 189 0 19 483 8 472 0.0 592
Msa1364260 AT1G71140.1 60.087 461 179 1 23 483 12 467 0.0 576
Msa1364260 AT1G15180.1 59.006 483 191 1 1 483 1 476 0.0 568
Msa1364260 AT1G15170.2 65.169 356 124 0 128 483 1 356 1.69e-176 500
Msa1364260 AT1G15150.2 62.640 356 133 0 128 483 1 356 1.59e-171 488
Msa1364260 AT2G04050.1 53.846 455 206 1 40 494 25 475 6.62e-164 473
Msa1364260 AT2G04080.1 52.079 457 213 3 40 495 25 476 3.95e-163 471
Msa1364260 AT2G04100.1 49.372 478 233 2 8 483 1 471 1.60e-160 464
Msa1364260 AT2G04040.1 51.828 465 216 4 33 494 16 475 4.15e-160 463
Msa1364260 AT2G04080.2 49.787 470 222 4 8 474 1 459 2.49e-158 458
Msa1364260 AT2G04070.1 51.435 453 216 1 42 494 27 475 8.32e-158 457
Msa1364260 AT2G04100.2 48.950 476 233 3 8 480 1 469 1.90e-156 453
Msa1364260 AT2G04090.1 48.899 454 232 0 30 483 18 471 1.34e-155 451
Msa1364260 AT1G66760.2 47.817 481 240 3 5 485 2 471 9.86e-155 449
Msa1364260 AT1G66780.1 48.596 463 238 0 24 486 17 479 3.69e-153 446
Msa1364260 AT5G52450.1 49.677 465 228 1 25 483 3 467 1.05e-152 444
Msa1364260 AT1G64820.1 45.155 485 256 2 11 493 6 482 5.97e-151 441
Msa1364260 AT1G66760.1 47.357 473 238 3 5 477 2 463 2.49e-149 435
Msa1364260 AT1G73700.1 49.661 443 223 0 41 483 23 465 1.72e-148 433
Msa1364260 AT2G34360.1 47.495 459 228 4 25 482 15 461 7.18e-145 424
Msa1364260 AT1G15180.2 63.929 280 101 0 128 407 1 280 6.20e-127 376
Msa1364260 AT1G66760.4 48.387 403 197 3 5 407 2 393 4.38e-126 373
Msa1364260 AT2G34360.2 41.485 458 215 2 25 482 15 419 1.38e-120 360
Msa1364260 AT1G66760.3 48.949 333 170 0 145 477 3 335 2.85e-105 318
Msa1364260 AT3G23560.1 37.687 467 288 1 14 477 8 474 2.32e-101 313
Msa1364260 AT3G23550.1 38.261 460 280 3 19 475 6 464 7.24e-99 306
Msa1364260 AT1G61890.1 36.878 442 279 0 42 483 47 488 4.66e-98 305
Msa1364260 AT3G21690.1 38.235 442 273 0 42 483 52 493 2.03e-97 303
Msa1364260 AT1G61890.2 35.991 464 290 3 18 474 16 479 5.35e-96 299
Msa1364260 AT1G11670.1 34.343 495 309 4 1 483 1 491 9.78e-95 296
Msa1364260 AT1G61890.3 35.435 460 289 4 18 470 16 474 7.71e-89 280
Msa1364260 AT3G26590.1 35.794 447 282 3 39 483 42 485 2.83e-87 277
Msa1364260 AT5G38030.1 33.676 487 314 4 3 483 2 485 6.04e-87 276
Msa1364260 AT3G59030.1 34.082 490 302 7 10 483 8 492 2.24e-85 272
Msa1364260 AT1G23300.1 34.774 486 309 4 3 483 2 484 2.54e-85 272
Msa1364260 AT1G12950.1 36.036 444 279 3 42 483 67 507 2.69e-85 272
Msa1364260 AT5G44050.1 34.990 483 307 5 4 483 3 481 9.72e-85 270
Msa1364260 AT1G71870.1 31.590 497 317 5 17 493 1 494 9.67e-84 268
Msa1364260 AT4G29140.1 33.958 480 313 4 3 480 17 494 1.82e-81 263
Msa1364260 AT4G21910.2 35.347 447 278 4 42 483 57 497 3.07e-81 261
Msa1364260 AT4G21910.4 35.347 447 278 4 42 483 57 497 3.07e-81 261
Msa1364260 AT4G21903.1 35.593 413 263 2 72 483 83 493 6.46e-81 260
Msa1364260 AT4G21910.3 35.123 447 279 4 42 483 55 495 8.27e-81 260
Msa1364260 AT4G21910.1 35.123 447 279 4 42 483 55 495 8.27e-81 260
Msa1364260 AT1G47530.1 34.884 473 295 8 17 483 10 475 1.09e-79 256
Msa1364260 AT1G58340.1 32.510 486 309 6 9 481 18 497 1.47e-79 258
Msa1364260 AT3G23560.2 36.524 397 249 1 14 407 8 404 6.84e-79 253
Msa1364260 AT4G21903.2 34.845 419 266 3 72 485 83 499 3.48e-78 254
Msa1364260 AT4G21903.3 35.677 384 247 0 100 483 1 384 1.26e-77 248
Msa1364260 AT5G65380.1 33.824 476 310 3 9 483 7 478 1.03e-76 249
Msa1364260 AT3G26590.2 36.458 384 243 1 100 483 1 383 6.90e-76 244
Msa1364260 AT4G23030.1 33.547 468 300 5 20 481 10 472 1.81e-75 246
Msa1364260 AT5G19700.1 30.480 479 328 1 3 481 2 475 1.88e-74 244
Msa1364260 AT5G52050.1 31.084 489 318 5 1 480 1 479 3.78e-74 243
Msa1364260 AT2G38510.1 31.757 444 298 5 41 481 5 446 1.41e-73 241
Msa1364260 AT5G10420.1 34.120 466 299 4 20 483 20 479 4.65e-73 239
Msa1364260 AT1G33090.1 34.454 476 301 5 1 474 1 467 7.09e-73 239
Msa1364260 AT1G33110.1 32.981 473 311 4 13 483 8 476 1.13e-71 236
Msa1364260 AT1G33100.1 34.120 466 294 6 13 474 8 464 7.18e-71 234
Msa1364260 AT1G33080.1 32.784 485 315 5 1 483 1 476 4.74e-70 232
Msa1364260 AT4G00350.1 34.009 444 287 3 42 483 88 527 7.97e-70 233
Msa1364260 AT1G33080.2 32.573 482 314 5 1 480 1 473 1.09e-69 231
Msa1364260 AT1G47530.2 37.179 390 232 8 100 483 1 383 8.94e-69 225
Msa1364260 AT5G49130.1 32.166 457 292 7 41 481 25 479 2.39e-68 228
Msa1364260 AT3G59030.2 33.575 414 254 7 10 407 8 416 7.09e-66 219
Msa1364260 AT4G25640.1 33.190 464 307 2 20 483 13 473 1.96e-64 217
Msa1364260 AT4G25640.2 33.190 464 307 2 20 483 13 473 4.11e-64 216
Msa1364260 AT4G25640.3 33.412 425 280 2 20 444 13 434 1.25e-60 206
Msa1364260 AT4G22790.1 29.915 468 314 4 25 480 5 470 8.64e-57 196
Msa1364260 AT3G03620.2 28.184 479 333 6 18 492 17 488 4.40e-50 179
Msa1364260 AT3G03620.1 28.184 479 333 6 18 492 17 488 4.40e-50 179
Msa1364260 AT1G33110.2 31.156 398 268 4 13 408 8 401 6.62e-50 176
Msa1364260 AT5G17700.2 28.512 477 330 5 8 483 8 474 3.36e-49 176
Msa1364260 AT5G17700.1 28.512 477 330 5 8 483 8 474 3.36e-49 176
Msa1364260 AT2G04066.1 51.181 127 62 0 361 487 41 167 1.19e-38 139
Msa1364260 AT5G17700.3 27.681 401 279 5 8 407 8 398 5.43e-34 132

Find 109 sgRNAs with CRISPR-Local

Find 308 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATAACATGTTGTATAAAT+TGG 0.106809 tig0018115:-16032 Msa1364260:CDS
TGGAACTATTTCAAGGAATT+TGG 0.108281 tig0018115:-17424 Msa1364260:CDS
AATTGTCGCCGTAACAAATT+TGG 0.138232 tig0018115:+15353 None:intergenic
TTGCTGCTGCATTGGCATTT+TGG 0.153192 tig0018115:-16128 Msa1364260:CDS
TTTGCATGCCGCCATGTTTA+TGG 0.161252 tig0018115:-16502 Msa1364260:CDS
GCCTTAGCCATTTCCTTTAC+TGG 0.165328 tig0018115:-20123 Msa1364260:CDS
GCGACAATTCCCCACTTTAT+TGG 0.177279 tig0018115:-15339 Msa1364260:intron
TTTACTAATGCAGGCAATTA+AGG 0.237076 tig0018115:-15444 Msa1364260:intron
AAGCAGGAAGATTCACAATT+TGG 0.240770 tig0018115:-17715 Msa1364260:CDS
ACAAGGGTTTCAAATGAATT+AGG 0.244096 tig0018115:-16613 Msa1364260:CDS
TGAAATAGTTCCATAGAAAT+TGG 0.245398 tig0018115:+17434 None:intergenic
TTCTTCTTCTTCTTTGAAAT+GGG 0.294950 tig0018115:-20387 Msa1364260:intron
GAAGTTTCAAGTTGTGGATT+AGG 0.295229 tig0018115:+16907 None:intergenic
GGAACTATTTCAAGGAATTT+GGG 0.312614 tig0018115:-17423 Msa1364260:CDS
AAGACAGGATTGAGTAATAT+TGG 0.317710 tig0018115:-17551 Msa1364260:CDS
AACTAGAGCGCCAATTTCTA+TGG 0.323580 tig0018115:-17444 Msa1364260:CDS
TGTCACCGGTTTCAGCTTCC+TGG 0.324030 tig0018115:-20100 Msa1364260:intron
AAGAGGATTTGTTACCTATC+AGG 0.328940 tig0018115:-20240 Msa1364260:CDS
GAAAGAATAGAGAAATTAGT+TGG 0.332532 tig0018115:-20284 Msa1364260:CDS
TCAGGGGTTGCTAGAGGTTG+TGG 0.334964 tig0018115:-16210 Msa1364260:intron
CCTGCTTCATGTGCAATTAG+AGG 0.339106 tig0018115:+17731 None:intergenic
TATACCTCTTTGCTGGGCTT+TGG 0.339839 tig0018115:-17579 Msa1364260:CDS
TACCTTCTGCTGTGATGGTT+TGG 0.347556 tig0018115:-17382 Msa1364260:intron
GTATCCATATACCTCTTTGC+TGG 0.350676 tig0018115:-17586 Msa1364260:CDS
ACAACATGTGGACAGGCTTA+TGG 0.351982 tig0018115:-17881 Msa1364260:CDS
ATTAATAATCTCCAATAAAG+TGG 0.354532 tig0018115:+15328 None:intergenic
AAACTTCAGTTCTATCAGTT+TGG 0.355601 tig0018115:-16891 Msa1364260:intron
CGCTATACCTTCTGCTGTGA+TGG 0.376002 tig0018115:-17387 Msa1364260:CDS
AAGCCCAGCAAAGAGGTATA+TGG 0.387347 tig0018115:+17582 None:intergenic
TATACAATACCATTTGGAAT+TGG 0.396274 tig0018115:-16751 Msa1364260:CDS
CAGGGGTTGCTAGAGGTTGT+GGG 0.399326 tig0018115:-16209 Msa1364260:CDS
GATTCCTGTTGCTGCTGCAT+TGG 0.404386 tig0018115:-16136 Msa1364260:CDS
GAGCTAATTGTCTTGCTCTC+TGG 0.405045 tig0018115:-16937 Msa1364260:CDS
ATTGTCGCCGTAACAAATTT+GGG 0.406688 tig0018115:+15354 None:intergenic
CGTCACTGTCTTGGCTCCTC+TGG 0.408539 tig0018115:-16443 Msa1364260:CDS
CATAACATGTTGTATAAATT+GGG 0.412639 tig0018115:-16031 Msa1364260:CDS
AAAAGAATACGGATTGAGAT+TGG 0.414241 tig0018115:+20421 None:intergenic
TTGTATAAATTGGGAGCAAC+TGG 0.416206 tig0018115:-16022 Msa1364260:intron
TGGGTTCAAGTGGGAGGAAA+AGG 0.421749 tig0018115:-16108 Msa1364260:CDS
TTGCAATGAGCATTTCCATT+TGG 0.422457 tig0018115:-17520 Msa1364260:CDS
ATTTCTATGGAACTATTTCA+AGG 0.422594 tig0018115:-17431 Msa1364260:CDS
TATCCATATACCTCTTTGCT+GGG 0.424522 tig0018115:-17585 Msa1364260:CDS
GCTAAACAATATCAAAGAAT+AGG 0.430267 tig0018115:-17857 Msa1364260:CDS
ACTCTCTATACAATACCATT+TGG 0.442113 tig0018115:-16757 Msa1364260:CDS
AAAGAATAGAGAAATTAGTT+GGG 0.445277 tig0018115:-20283 Msa1364260:CDS
ATAGGAGTTTATGTGAATCT+AGG 0.460939 tig0018115:-16177 Msa1364260:CDS
AGGAATCCCACATAGGTAGA+AGG 0.467641 tig0018115:+16152 None:intergenic
TTGTTACCTATCAGGTCCTA+TGG 0.468502 tig0018115:-20232 Msa1364260:CDS
ACGGCAACACGTGCTTCAAA+TGG 0.468974 tig0018115:+16580 None:intergenic
AAGTGGGAGGAAAAGGACTT+TGG 0.475738 tig0018115:-16101 Msa1364260:CDS
CTGCTTCATGTGCAATTAGA+GGG 0.480437 tig0018115:+17732 None:intergenic
AGAACTGAAGTTTCAAGTTG+TGG 0.486860 tig0018115:+16901 None:intergenic
AGCATACAAGGCGTTCTCGC+AGG 0.492812 tig0018115:-16397 Msa1364260:intron
AATGCCAATGCAGCAGCAAC+AGG 0.494774 tig0018115:+16132 None:intergenic
GGGTGAAGAGAGAAGAGTGA+TGG 0.495934 tig0018115:-20367 Msa1364260:CDS
TTAATAATCTCCAATAAAGT+GGG 0.496362 tig0018115:+15329 None:intergenic
ACACAAGAGCTTAAAAGCAT+TGG 0.497017 tig0018115:+17617 None:intergenic
GGTCAAGCCCAAATTTGTTA+CGG 0.497827 tig0018115:-15361 Msa1364260:three_prime_UTR
TGTTAAATACGTCACTGTCT+TGG 0.503008 tig0018115:-16452 Msa1364260:CDS
TGGGCTTTGGTATTCAAGAC+AGG 0.506637 tig0018115:-17566 Msa1364260:CDS
CCTCTAATTGCACATGAAGC+AGG 0.510834 tig0018115:-17731 Msa1364260:CDS
ATCATGATTGTTGGTCACTT+AGG 0.513601 tig0018115:-20168 Msa1364260:CDS
TTTACACGAAAGAATTGAAG+AGG 0.519436 tig0018115:-20257 Msa1364260:CDS
GCTTACCAGGAAGCTGAAAC+CGG 0.522233 tig0018115:+20095 None:intergenic
CAGCTACCATAGGACCTGAT+AGG 0.522912 tig0018115:+20226 None:intergenic
GGTGACACCAGTAAAGGAAA+TGG 0.523197 tig0018115:+20116 None:intergenic
GGAGGAAAAGGACTTTGGAT+TGG 0.526302 tig0018115:-16096 Msa1364260:CDS
ATTTCCTTTACTGGTGTCAC+CGG 0.526363 tig0018115:-20114 Msa1364260:CDS
GTTTCAAATGAATTAGGAGC+TGG 0.528676 tig0018115:-16607 Msa1364260:CDS
GTTGTGTCCATCATGATTGT+TGG 0.531106 tig0018115:-20177 Msa1364260:CDS
GTTATACTCGACAGCATACA+AGG 0.534553 tig0018115:-16409 Msa1364260:CDS
ATAGATTGGTATGATAAGAA+AGG 0.548860 tig0018115:-15382 Msa1364260:exon
AAGATATATAAATATAGAGT+CGG 0.554707 tig0018115:+20449 None:intergenic
ATTTGAAAATATCTAACTAG+TGG 0.558930 tig0018115:+17656 None:intergenic
ACCAGTAAAGGAAATGGCTA+AGG 0.566283 tig0018115:+20122 None:intergenic
CTAGGAGCCTTCTACCTATG+TGG 0.568455 tig0018115:-16159 Msa1364260:CDS
AACATGGCGGCATGCAAAGA+GGG 0.570292 tig0018115:+16507 None:intergenic
GCTGCAGCACCAATTCCAAA+TGG 0.574096 tig0018115:+16742 None:intergenic
TACCAAACCATCACAGCAGA+AGG 0.580285 tig0018115:+17380 None:intergenic
TAGGAGCCTTCTACCTATGT+GGG 0.586277 tig0018115:-16158 Msa1364260:CDS
ATTTATTCAGGGGTTGCTAG+AGG 0.587635 tig0018115:-16216 Msa1364260:intron
CTTTGGATTGGTATACAAGT+CGG 0.590240 tig0018115:-16084 Msa1364260:CDS
ACTAGAAACAACATGTGGAC+AGG 0.594187 tig0018115:-17888 Msa1364260:CDS
TAAGTGACCAACAATCATGA+TGG 0.595343 tig0018115:+20170 None:intergenic
CTCTGAATATTATAGTACAA+GGG 0.600517 tig0018115:-16629 Msa1364260:intron
TTGATGATTGTAGCCTTGAG+TGG 0.607253 tig0018115:-16969 Msa1364260:intron
GGTTGTGGGTGGCAACACAT+AGG 0.610292 tig0018115:-16195 Msa1364260:CDS
TAACAGATGGGAATGGCTAG+TGG 0.610311 tig0018115:-17911 Msa1364260:intron
GCTCATATGACCACCACTCA+AGG 0.611921 tig0018115:+16956 None:intergenic
GAAACCGGTGACACCAGTAA+AGG 0.616842 tig0018115:+20110 None:intergenic
TTGGAATTGGTGCTGCAGCA+AGG 0.618649 tig0018115:-16738 Msa1364260:intron
AAACATGGCGGCATGCAAAG+AGG 0.622373 tig0018115:+16506 None:intergenic
TTGATCCAAATAAAAGAGAG+TGG 0.624093 tig0018115:+17788 None:intergenic
GGGTTGCTAGAGGTTGTGGG+TGG 0.624099 tig0018115:-16206 Msa1364260:CDS
AAGTGACATAGCAGCTAACA+CGG 0.624301 tig0018115:+16561 None:intergenic
ATGATTGTAGCCTTGAGTGG+TGG 0.624582 tig0018115:-16966 Msa1364260:intron
AATACCAAAGCCCAGCAAAG+AGG 0.626723 tig0018115:+17575 None:intergenic
ACAGGATTGAGTAATATTGG+TGG 0.628730 tig0018115:-17548 Msa1364260:CDS
GAAGATGTTACAGCTACCAT+AGG 0.629703 tig0018115:+20216 None:intergenic
AGTAAAGGAAATGGCTAAGG+CGG 0.639044 tig0018115:+20125 None:intergenic
CAGCAACAGGAATCCCACAT+AGG 0.643167 tig0018115:+16145 None:intergenic
TATTGTATAGAGAGTTGCAA+TGG 0.643527 tig0018115:+16765 None:intergenic
TGAAAATGTAGCCATAAACA+TGG 0.657665 tig0018115:+16491 None:intergenic
TAATAATCTCCAATAAAGTG+GGG 0.657894 tig0018115:+15330 None:intergenic
ATAACAGATATAGAAACCAG+AGG 0.672251 tig0018115:+16427 None:intergenic
CTCATGCTAACAGATGGGAA+TGG 0.678937 tig0018115:-17918 Msa1364260:intron
AGTGGACTAGAAACAACATG+TGG 0.725232 tig0018115:-17893 Msa1364260:CDS
AAATGTAGCCATAAACATGG+CGG 0.738991 tig0018115:+16494 None:intergenic
AGGCAATTAAGGCTAGACAG+AGG 0.770785 tig0018115:-15433 Msa1364260:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATAAATTAGACAAATA+CGG - tig0018115:15880-15899 Msa1364260:intron 10.0%
!! TATAATATACACAAATAAAA+TGG - tig0018115:16712-16731 Msa1364260:intron 10.0%
!! AATATATCATTCAAATTTAA+TGG + tig0018115:18681-18700 None:intergenic 10.0%
!! AAGATATATAAATATAGAGT+CGG + tig0018115:15236-15255 None:intergenic 15.0%
!! ATTATAAATTAGACAAATAC+GGG - tig0018115:15881-15900 Msa1364260:intron 15.0%
!!! AAGTTAGATATTTTATAAGT+AGG - tig0018115:16512-16531 Msa1364260:CDS 15.0%
!!! TGATTGTTTTATGTTTAATA+TGG - tig0018115:16592-16611 Msa1364260:CDS 15.0%
!!! GATTGTTTTATGTTTAATAT+GGG - tig0018115:16593-16612 Msa1364260:CDS 15.0%
!! TATTTGTGTATATTATACAA+AGG + tig0018115:16709-16728 None:intergenic 15.0%
!! TGTTTACTATTTATTTATTC+AGG - tig0018115:19454-19473 Msa1364260:intron 15.0%
!! GTTTACTATTTATTTATTCA+GGG - tig0018115:19455-19474 Msa1364260:intron 15.0%
!! TTTACTATTTATTTATTCAG+GGG - tig0018115:19456-19475 Msa1364260:intron 15.0%
!!! TTACTATTTCGTAAATTTTA+GGG + tig0018115:19880-19899 None:intergenic 15.0%
!!! TTTACTATTTCGTAAATTTT+AGG + tig0018115:19881-19900 None:intergenic 15.0%
!! ATTAATAAATAATGCCAAAA+AGG + tig0018115:20204-20223 None:intergenic 15.0%
!!! TTATTTTAATTAGACAGATA+GGG - tig0018115:20392-20411 Msa1364260:intron 15.0%
!! AAAGAATAGAGAAATTAGTT+GGG - tig0018115:15399-15418 Msa1364260:CDS 20.0%
!! TTAAACAAAAGACTATGAAT+AGG + tig0018115:15980-15999 None:intergenic 20.0%
!!! AATTAAAAGTTTTGAGTAAC+CGG - tig0018115:16363-16382 Msa1364260:intron 20.0%
!!! TTTTAATGGACAAACAATAA+TGG + tig0018115:16430-16449 None:intergenic 20.0%
!!! AAAGAAACAGTAAAGTTTTA+AGG + tig0018115:16655-16674 None:intergenic 20.0%
!!! TAAAACTTTACTGTTTCTTT+TGG - tig0018115:16655-16674 Msa1364260:intron 20.0%
!! AAGACATTTGTATATAGTAT+AGG + tig0018115:16739-16758 None:intergenic 20.0%
!! GTCTTCTATTATATACAAAT+TGG + tig0018115:16836-16855 None:intergenic 20.0%
!!! AGTATCTTTGATCAATTTTA+TGG + tig0018115:16875-16894 None:intergenic 20.0%
!! CAAAAAAGAAAAAATCATGA+AGG + tig0018115:17150-17169 None:intergenic 20.0%
!! AATTTGCATAATAATCATGA+AGG + tig0018115:17181-17200 None:intergenic 20.0%
!!! ATTTTTCATAGTCTTATCTT+TGG - tig0018115:17285-17304 Msa1364260:intron 20.0%
!!! TATCAAAGTCATATTTTGTA+GGG - tig0018115:17530-17549 Msa1364260:CDS 20.0%
!!! ATCTATATTTTCTCTCATAT+TGG - tig0018115:17863-17882 Msa1364260:CDS 20.0%
!! ATTTGAAAATATCTAACTAG+TGG + tig0018115:18029-18048 None:intergenic 20.0%
!! AAAAATCACATTTAACCAAA+TGG + tig0018115:18180-18199 None:intergenic 20.0%
!!! AGATCTTTCATTAGTTTTAA+TGG + tig0018115:18468-18487 None:intergenic 20.0%
!!! TTGCAATTTTTTAAATTCGA+TGG - tig0018115:18531-18550 Msa1364260:intron 20.0%
!! AAAGATAATCTGTTAGAATT+AGG - tig0018115:19365-19384 Msa1364260:intron 20.0%
!! AAGATAATCTGTTAGAATTA+GGG - tig0018115:19366-19385 Msa1364260:intron 20.0%
!! TCATAACATGTTGTATAAAT+TGG - tig0018115:19650-19669 Msa1364260:intron 20.0%
!! CATAACATGTTGTATAAATT+GGG - tig0018115:19651-19670 Msa1364260:intron 20.0%
!!! CACAATAAATTTGAAATTTG+AGG + tig0018115:19712-19731 None:intergenic 20.0%
!! TATATAACTTATAGCCAATT+TGG - tig0018115:19759-19778 Msa1364260:intron 20.0%
!!! AGAGAAAATTTTATCCAAAT+TGG + tig0018115:19776-19795 None:intergenic 20.0%
!! ATTTATTAATCTCAATTGTG+TGG - tig0018115:20214-20233 Msa1364260:CDS 20.0%
!! TTAATAATCTCCAATAAAGT+GGG + tig0018115:20356-20375 None:intergenic 20.0%
!! ATTAATAATCTCCAATAAAG+TGG + tig0018115:20357-20376 None:intergenic 20.0%
!!! CTTATTTTAATTAGACAGAT+AGG - tig0018115:20391-20410 Msa1364260:intron 20.0%
! GAAGAAGAAGAAAAAGAATA+CGG + tig0018115:15275-15294 None:intergenic 25.0%
!!! TTCTTCTTCTTCTTTGAAAT+GGG - tig0018115:15295-15314 Msa1364260:intron 25.0%
! GAAAGAATAGAGAAATTAGT+TGG - tig0018115:15398-15417 Msa1364260:CDS 25.0%
!! ATCTTCACAATATCTTTTAC+AGG - tig0018115:15483-15502 Msa1364260:intron 25.0%
!! AATCTAGTAATTTTTGTCCT+CGG + tig0018115:15643-15662 None:intergenic 25.0%
! AGGACAAAAATTACTAGATT+TGG - tig0018115:15643-15662 Msa1364260:intron 25.0%
! TAGTTGATTATACTCCTATT+TGG - tig0018115:15775-15794 Msa1364260:intron 25.0%
!! AATACGGGTACATTTTTATA+AGG - tig0018115:15896-15915 Msa1364260:intron 25.0%
! CTTCTTTAGTCAAAATAACT+TGG + tig0018115:15929-15948 None:intergenic 25.0%
! AGCAAATGAAAATTGTGAAT+TGG - tig0018115:15947-15966 Msa1364260:intron 25.0%
! GTCCATTAAAAAATGAAGTT+AGG - tig0018115:16439-16458 Msa1364260:CDS 25.0%
!!! AGCCTAACTTCATTTTTTAA+TGG + tig0018115:16444-16463 None:intergenic 25.0%
! TAAAAAATGAAGTTAGGCTT+AGG - tig0018115:16445-16464 Msa1364260:CDS 25.0%
!!! AAACAGTAAAGTTTTAAGGT+AGG + tig0018115:16651-16670 None:intergenic 25.0%
! AAGAAAAAAATACACCTTGA+AGG + tig0018115:16954-16973 None:intergenic 25.0%
!!! TGTTTTATTATGTCAAGATG+TGG - tig0018115:17054-17073 Msa1364260:intron 25.0%
!! CGTTTTTACGATTGTTAAAT+TGG + tig0018115:17236-17255 None:intergenic 25.0%
! TTTAATAGTACACATGGTAT+TGG + tig0018115:17350-17369 None:intergenic 25.0%
! TTACTAGTATTTAGCTAACT+AGG - tig0018115:17399-17418 Msa1364260:CDS 25.0%
!!! TATTAGCTTGCTCAAAAATT+AGG - tig0018115:17450-17469 Msa1364260:CDS 25.0%
!!! GTCTTATAAACTAGTTTGTT+TGG - tig0018115:17480-17499 Msa1364260:CDS 25.0%
!! GTATCAAAGTCATATTTTGT+AGG - tig0018115:17529-17548 Msa1364260:CDS 25.0%
! AATATATACATTGCACAACT+CGG + tig0018115:17644-17663 None:intergenic 25.0%
! GCTAAACAATATCAAAGAAT+AGG - tig0018115:17825-17844 Msa1364260:CDS 25.0%
!! TGGTTAAATGTGATTTTTCT+TGG - tig0018115:18182-18201 Msa1364260:intron 25.0%
! TGAAATAGTTCCATAGAAAT+TGG + tig0018115:18251-18270 None:intergenic 25.0%
! ATTTCTATGGAACTATTTCA+AGG - tig0018115:18251-18270 Msa1364260:intron 25.0%
! ATATACAAGTTTAGTTGAAG+AGG + tig0018115:18337-18356 None:intergenic 25.0%
! TTTCTGTTCTCAAAATTAGA+AGG - tig0018115:18381-18400 Msa1364260:intron 25.0%
! TTCTGTTCTCAAAATTAGAA+GGG - tig0018115:18382-18401 Msa1364260:intron 25.0%
! CTATCAAAAGATCTATTTAG+CGG - tig0018115:18438-18457 Msa1364260:intron 25.0%
!! ATATTCTTTTACTGCATCAA+TGG - tig0018115:18502-18521 Msa1364260:intron 25.0%
!! TATACAATACCATTTGGAAT+TGG - tig0018115:18931-18950 Msa1364260:intron 25.0%
! TCTCTGAATATTATAGTACA+AGG - tig0018115:19052-19071 Msa1364260:intron 25.0%
! CTCTGAATATTATAGTACAA+GGG - tig0018115:19053-19072 Msa1364260:intron 25.0%
! AGATAATCTGTTAGAATTAG+GGG - tig0018115:19367-19386 Msa1364260:intron 25.0%
!! AATTTGAGGTTTCAAGATAA+AGG + tig0018115:19698-19717 None:intergenic 25.0%
!! TGCTTTGATTGACAATTTTA+AGG - tig0018115:19730-19749 Msa1364260:intron 25.0%
! TAAGGAAATATTTCGTTTCA+AGG + tig0018115:19941-19960 None:intergenic 25.0%
! TCAATATCTAAGTCTTTCTA+TGG + tig0018115:19972-19991 None:intergenic 25.0%
!! CAATATAACATTTAAAACCG+CGG - tig0018115:20023-20042 Msa1364260:intron 25.0%
! AAATTTATTCGATGATCATG+TGG - tig0018115:20172-20191 Msa1364260:CDS 25.0%
!! TTGTTTGATGTTCAATTTTC+TGG - tig0018115:20272-20291 Msa1364260:CDS 25.0%
! ATAGATTGGTATGATAAGAA+AGG - tig0018115:20300-20319 Msa1364260:CDS 25.0%
! TAATAATCTCCAATAAAGTG+GGG + tig0018115:20355-20374 None:intergenic 25.0%
! AAAAGAATACGGATTGAGAT+TGG + tig0018115:15264-15283 None:intergenic 30.0%
! CTTCTTCTTCTTCTTTGAAA+TGG - tig0018115:15294-15313 Msa1364260:three_prime_UTR 30.0%
TTTACACGAAAGAATTGAAG+AGG - tig0018115:15425-15444 Msa1364260:CDS 30.0%
AGAAAAATAGGCAACCAAAT+AGG + tig0018115:15792-15811 None:intergenic 30.0%
CAAGATAGTAGCAGAAAAAT+AGG + tig0018115:15804-15823 None:intergenic 30.0%
ATCACTGTGAAGTTAAACAA+AGG + tig0018115:16309-16328 None:intergenic 30.0%
AAGACTATAACTCATGAAGA+GGG - tig0018115:16388-16407 Msa1364260:intron 30.0%
! TAATGGACAAACAATAATGG+CGG + tig0018115:16427-16446 None:intergenic 30.0%
AAATCATGAAGGTTCAGTAT+AGG + tig0018115:17139-17158 None:intergenic 30.0%
TTAAATTGGATACATGTTCC+TGG + tig0018115:17222-17241 None:intergenic 30.0%
GTCTAACATACAATCATGAT+CGG + tig0018115:17318-17337 None:intergenic 30.0%
CAATGCTTTAATAGTACACA+TGG + tig0018115:17356-17375 None:intergenic 30.0%
!! TTAAAGCATTGAATTGACAG+TGG - tig0018115:17365-17384 Msa1364260:intron 30.0%
AGATCTAAGTCCATTTAACA+TGG - tig0018115:17507-17526 Msa1364260:CDS 30.0%
GACTTTGATACCATGTTAAA+TGG + tig0018115:17520-17539 None:intergenic 30.0%
! TCATATTTTGTAGGGTATGT+GGG - tig0018115:17538-17557 Msa1364260:CDS 30.0%
ATATATACATTGCACAACTC+GGG + tig0018115:17643-17662 None:intergenic 30.0%
ATCAATGCATTGAGTAACTT+GGG + tig0018115:17737-17756 None:intergenic 30.0%
! GTTTACCACTCTCTTTTATT+TGG - tig0018115:17889-17908 Msa1364260:CDS 30.0%
TTGATCCAAATAAAAGAGAG+TGG + tig0018115:17897-17916 None:intergenic 30.0%
AAGACAGGATTGAGTAATAT+TGG - tig0018115:18131-18150 Msa1364260:intron 30.0%
TGGAACTATTTCAAGGAATT+TGG - tig0018115:18258-18277 Msa1364260:intron 30.0%
GGAACTATTTCAAGGAATTT+GGG - tig0018115:18259-18278 Msa1364260:intron 30.0%
TTTAGTGCTTGAAATCGTAA+GGG + tig0018115:18600-18619 None:intergenic 30.0%
! TTTTAGTGCTTGAAATCGTA+AGG + tig0018115:18601-18620 None:intergenic 30.0%
AAACTTCAGTTCTATCAGTT+TGG - tig0018115:18791-18810 Msa1364260:intron 30.0%
TATTGTATAGAGAGTTGCAA+TGG + tig0018115:18920-18939 None:intergenic 30.0%
ACTCTCTATACAATACCATT+TGG - tig0018115:18925-18944 Msa1364260:intron 30.0%
ACAAGGGTTTCAAATGAATT+AGG - tig0018115:19069-19088 Msa1364260:intron 30.0%
TGAAAATGTAGCCATAAACA+TGG + tig0018115:19194-19213 None:intergenic 30.0%
ATAACAGATATAGAAACCAG+AGG + tig0018115:19258-19277 None:intergenic 30.0%
ATAGGAGTTTATGTGAATCT+AGG - tig0018115:19505-19524 Msa1364260:intron 30.0%
TATGGAGTAAATCAGCATTT+CGG - tig0018115:19822-19841 Msa1364260:intron 30.0%
CTTTCTATGGATGTTAACTA+AGG + tig0018115:19959-19978 None:intergenic 30.0%
TTTACTAATGCAGGCAATTA+AGG - tig0018115:20238-20257 Msa1364260:CDS 30.0%
!! ATTTTCTGGAGAGAATAGAT+TGG - tig0018115:20286-20305 Msa1364260:CDS 30.0%
!! AAGAGGATTTGTTACCTATC+AGG - tig0018115:15442-15461 Msa1364260:intron 35.0%
TAAGTGACCAACAATCATGA+TGG + tig0018115:15515-15534 None:intergenic 35.0%
ATCATGATTGTTGGTCACTT+AGG - tig0018115:15514-15533 Msa1364260:intron 35.0%
CTTGTGTGACATTAATGTCA+CGG - tig0018115:16039-16058 Msa1364260:CDS 35.0%
TTAAATCTGGACCACACATT+AGG - tig0018115:16067-16086 Msa1364260:CDS 35.0%
ATCTAATGTCACCTAATGTG+TGG + tig0018115:16081-16100 None:intergenic 35.0%
TTGTCATTGTCATTGCATCT+GGG + tig0018115:16201-16220 None:intergenic 35.0%
ATTGTCATTGTCATTGCATC+TGG + tig0018115:16202-16221 None:intergenic 35.0%
CTGTGAAGTTAAACAAAGGA+AGG + tig0018115:16305-16324 None:intergenic 35.0%
ACAGTGATTGATGTGTATGT+GGG - tig0018115:16321-16340 Msa1364260:intron 35.0%
TCATGAGTTATAGTCTTCAC+CGG + tig0018115:16385-16404 None:intergenic 35.0%
GAAGACTATAACTCATGAAG+AGG - tig0018115:16387-16406 Msa1364260:intron 35.0%
AAGAGTTGTGTAAACTAGTG+AGG - tig0018115:16468-16487 Msa1364260:CDS 35.0%
AACGTTAGATACTCCTATTG+TGG - tig0018115:16913-16932 Msa1364260:CDS 35.0%
AGGTTCAGTATAGGTGAAAT+GGG + tig0018115:17130-17149 None:intergenic 35.0%
AAGGTTCAGTATAGGTGAAA+TGG + tig0018115:17131-17150 None:intergenic 35.0%
TAACAATCGTAAAAACGCTC+TGG - tig0018115:17239-17258 Msa1364260:intron 35.0%
! GTACACATGGTATTGGAATT+TGG + tig0018115:17343-17362 None:intergenic 35.0%
! GTCATATTTTGTAGGGTATG+TGG - tig0018115:17537-17556 Msa1364260:CDS 35.0%
GCTAACTCATAAGATGATGA+GGG + tig0018115:17677-17696 None:intergenic 35.0%
TCAATGCATTGAGTAACTTG+GGG + tig0018115:17736-17755 None:intergenic 35.0%
CATCAATGCATTGAGTAACT+TGG + tig0018115:17738-17757 None:intergenic 35.0%
ATTTCACTCATGCTAACAGA+TGG - tig0018115:17758-17777 Msa1364260:CDS 35.0%
TTTCACTCATGCTAACAGAT+GGG - tig0018115:17759-17778 Msa1364260:CDS 35.0%
!!! GAGAACATACTAGTTTTCAC+AGG - tig0018115:17921-17940 Msa1364260:intron 35.0%
AAGCAGGAAGATTCACAATT+TGG - tig0018115:17967-17986 Msa1364260:intron 35.0%
! ATTGCATATGCAAAAAGTGC+TGG + tig0018115:17999-18018 None:intergenic 35.0%
! ACACAAGAGCTTAAAAGCAT+TGG + tig0018115:18068-18087 None:intergenic 35.0%
TATCCATATACCTCTTTGCT+GGG - tig0018115:18097-18116 Msa1364260:intron 35.0%
ACAGGATTGAGTAATATTGG+TGG - tig0018115:18134-18153 Msa1364260:intron 35.0%
TTGCAATGAGCATTTCCATT+TGG - tig0018115:18162-18181 Msa1364260:intron 35.0%
!! CAGAAACGTTGATTCATTCA+TGG + tig0018115:18367-18386 None:intergenic 35.0%
! GCATCTTGTAGAACTCTTTT+GGG + tig0018115:18644-18663 None:intergenic 35.0%
! AGCATCTTGTAGAACTCTTT+TGG + tig0018115:18645-18664 None:intergenic 35.0%
!! GAAGTTTCAAGTTGTGGATT+AGG + tig0018115:18778-18797 None:intergenic 35.0%
!! AGAACTGAAGTTTCAAGTTG+TGG + tig0018115:18784-18803 None:intergenic 35.0%
GTTTCAAATGAATTAGGAGC+TGG - tig0018115:19075-19094 Msa1364260:intron 35.0%
AAATGTAGCCATAAACATGG+CGG + tig0018115:19191-19210 None:intergenic 35.0%
TGTTAAATACGTCACTGTCT+TGG - tig0018115:19230-19249 Msa1364260:intron 35.0%
GGTAAATTGCTCAGTATACA+AGG - tig0018115:19306-19325 Msa1364260:intron 35.0%
!! CTTTGGATTGGTATACAAGT+CGG - tig0018115:19598-19617 Msa1364260:intron 35.0%
TTGTATAAATTGGGAGCAAC+TGG - tig0018115:19660-19679 Msa1364260:intron 35.0%
! GTGCTATGTCACATTGATTA+TGG - tig0018115:19804-19823 Msa1364260:intron 35.0%
! TCATGTGGTAGAATCCTTTT+TGG - tig0018115:20187-20206 Msa1364260:CDS 35.0%
!! TTGTGTGGTTTTACTAATGC+AGG - tig0018115:20229-20248 Msa1364260:CDS 35.0%
ATTGTCGCCGTAACAAATTT+GGG + tig0018115:20331-20350 None:intergenic 35.0%
AATTGTCGCCGTAACAAATT+TGG + tig0018115:20332-20351 None:intergenic 35.0%
! GGGCATTTTTCTCATGATAA+AGG - tig0018115:20412-20431 Msa1364260:intron 35.0%
TTGTTACCTATCAGGTCCTA+TGG - tig0018115:15450-15469 Msa1364260:intron 40.0%
GAAGATGTTACAGCTACCAT+AGG + tig0018115:15469-15488 None:intergenic 40.0%
GTTGTGTCCATCATGATTGT+TGG - tig0018115:15505-15524 Msa1364260:intron 40.0%
AGTAAAGGAAATGGCTAAGG+CGG + tig0018115:15560-15579 None:intergenic 40.0%
ACCAGTAAAGGAAATGGCTA+AGG + tig0018115:15563-15582 None:intergenic 40.0%
!! ATTTCCTTTACTGGTGTCAC+CGG - tig0018115:15568-15587 Msa1364260:intron 40.0%
ACTCTCGAAAACTCAGTATC+TGG - tig0018115:15616-15635 Msa1364260:intron 40.0%
TATTGTATTTGTCCCTGCGT+GGG - tig0018115:16010-16029 Msa1364260:intron 40.0%
TGTCACGGTGACATTAAATC+TGG - tig0018115:16054-16073 Msa1364260:CDS 40.0%
GTGACATTAGATGTGTTGTG+TGG - tig0018115:16089-16108 Msa1364260:CDS 40.0%
TTGTCATTGCCATTGCATCT+GGG + tig0018115:16115-16134 None:intergenic 40.0%
ATTGTCATTGCCATTGCATC+TGG + tig0018115:16116-16135 None:intergenic 40.0%
GCAATGACAATGACAATGTG+TGG - tig0018115:16205-16224 Msa1364260:CDS 40.0%
AAAAGGACTCTCACGTATGT+GGG + tig0018115:16275-16294 None:intergenic 40.0%
AAAAAGGACTCTCACGTATG+TGG + tig0018115:16276-16295 None:intergenic 40.0%
!!! ACGTGAGAGTCCTTTTTTTC+CGG - tig0018115:16279-16298 Msa1364260:intron 40.0%
GAAGTTAAACAAAGGAAGGC+CGG + tig0018115:16301-16320 None:intergenic 40.0%
CACAGTGATTGATGTGTATG+TGG - tig0018115:16320-16339 Msa1364260:intron 40.0%
!!! GTTTTAAGGTAGGTCGTGTT+GGG + tig0018115:16641-16660 None:intergenic 40.0%
!!! AGTTTTAAGGTAGGTCGTGT+TGG + tig0018115:16642-16661 None:intergenic 40.0%
! TACTCCTATTGTGGATTGTG+TGG - tig0018115:16922-16941 Msa1364260:CDS 40.0%
! ACTCCTATTGTGGATTGTGT+GGG - tig0018115:16923-16942 Msa1364260:CDS 40.0%
ACATTCGCCCAAAAAAGATG+TGG - tig0018115:17084-17103 Msa1364260:intron 40.0%
GGTTCAGTATAGGTGAAATG+GGG + tig0018115:17129-17148 None:intergenic 40.0%
! TCCTTGAAGCACTATAAACC+AGG - tig0018115:17201-17220 Msa1364260:intron 40.0%
TCCTGGTTTATAGTGCTTCA+AGG + tig0018115:17205-17224 None:intergenic 40.0%
! CACATGGTATTGGAATTTGG+AGG + tig0018115:17340-17359 None:intergenic 40.0%
GGCTAACTCATAAGATGATG+AGG + tig0018115:17678-17697 None:intergenic 40.0%
TACGTATATCTCACTCTCAG+AGG + tig0018115:17699-17718 None:intergenic 40.0%
!! AGTGGACTAGAAACAACATG+TGG - tig0018115:17789-17808 Msa1364260:CDS 40.0%
ACTAGAAACAACATGTGGAC+AGG - tig0018115:17794-17813 Msa1364260:CDS 40.0%
CTGCTTCATGTGCAATTAGA+GGG + tig0018115:17953-17972 None:intergenic 40.0%
GTATCCATATACCTCTTTGC+TGG - tig0018115:18096-18115 Msa1364260:intron 40.0%
! AACTAGAGCGCCAATTTCTA+TGG - tig0018115:18238-18257 Msa1364260:intron 40.0%
! TTGATGATTGTAGCCTTGAG+TGG - tig0018115:18713-18732 Msa1364260:intron 40.0%
AAGTGACATAGCAGCTAACA+CGG + tig0018115:19124-19143 None:intergenic 40.0%
GTTATACTCGACAGCATACA+AGG - tig0018115:19273-19292 Msa1364260:intron 40.0%
ATTTATTCAGGGGTTGCTAG+AGG - tig0018115:19466-19485 Msa1364260:intron 40.0%
!!! ATTGGCATTTTGGGTTCAAG+TGG - tig0018115:19564-19583 Msa1364260:intron 40.0%
!!! TTGGCATTTTGGGTTCAAGT+GGG - tig0018115:19565-19584 Msa1364260:intron 40.0%
TTCCTTCAAATTTAGCACCG+CGG + tig0018115:20043-20062 None:intergenic 40.0%
!! GGTCAAGCCCAAATTTGTTA+CGG - tig0018115:20321-20340 Msa1364260:CDS 40.0%
GCCTTAGCCATTTCCTTTAC+TGG - tig0018115:15559-15578 Msa1364260:intron 45.0%
GGTGACACCAGTAAAGGAAA+TGG + tig0018115:15569-15588 None:intergenic 45.0%
CGAGAGTAGTATAGCTTACC+AGG + tig0018115:15603-15622 None:intergenic 45.0%
AAAACTCAGTATCTGGTCCG+AGG - tig0018115:15623-15642 Msa1364260:intron 45.0%
GTATTGTATTTGTCCCTGCG+TGG - tig0018115:16009-16028 Msa1364260:intron 45.0%
ATTGTATTTGTCCCTGCGTG+GGG - tig0018115:16011-16030 Msa1364260:intron 45.0%
AATGTGTGACCCAGATGCAA+TGG - tig0018115:16133-16152 Msa1364260:CDS 45.0%
ATTGCATCTGGGTCACACAA+CGG + tig0018115:16190-16209 None:intergenic 45.0%
AATGTGTGGCCCAGATACAA+TGG - tig0018115:16219-16238 Msa1364260:intron 45.0%
GTCACCGTACCATTGTATCT+GGG + tig0018115:16231-16250 None:intergenic 45.0%
TGTCACCGTACCATTGTATC+TGG + tig0018115:16232-16251 None:intergenic 45.0%
CAAAGGAAGGCCGGAAAAAA+AGG + tig0018115:16292-16311 None:intergenic 45.0%
CATCCCACACAATCCACAAT+AGG + tig0018115:16929-16948 None:intergenic 45.0%
!!! GGACTCGCCACATCTTTTTT+GGG + tig0018115:17094-17113 None:intergenic 45.0%
!!! TGGACTCGCCACATCTTTTT+TGG + tig0018115:17095-17114 None:intergenic 45.0%
GAAATGGGGACCACAAAAAG+TGG + tig0018115:17115-17134 None:intergenic 45.0%
CTTAGACTTAGATCCACGCT+AGG - tig0018115:17568-17587 Msa1364260:CDS 45.0%
ATCCACGCTAGGCAAAAATG+TGG - tig0018115:17579-17598 Msa1364260:CDS 45.0%
! ATCCACATTTTTGCCTAGCG+TGG + tig0018115:17584-17603 None:intergenic 45.0%
ATTGCACAACTCGGGAACAT+TGG + tig0018115:17635-17654 None:intergenic 45.0%
CTCATGCTAACAGATGGGAA+TGG - tig0018115:17764-17783 Msa1364260:CDS 45.0%
TAACAGATGGGAATGGCTAG+TGG - tig0018115:17771-17790 Msa1364260:CDS 45.0%
ACAACATGTGGACAGGCTTA+TGG - tig0018115:17801-17820 Msa1364260:CDS 45.0%
CCTGCTTCATGTGCAATTAG+AGG + tig0018115:17954-17973 None:intergenic 45.0%
CCTCTAATTGCACATGAAGC+AGG - tig0018115:17951-17970 Msa1364260:intron 45.0%
! AAGCCCAGCAAAGAGGTATA+TGG + tig0018115:18103-18122 None:intergenic 45.0%
TATACCTCTTTGCTGGGCTT+TGG - tig0018115:18103-18122 Msa1364260:intron 45.0%
AATACCAAAGCCCAGCAAAG+AGG + tig0018115:18110-18129 None:intergenic 45.0%
! TGGGCTTTGGTATTCAAGAC+AGG - tig0018115:18116-18135 Msa1364260:intron 45.0%
TACCTTCTGCTGTGATGGTT+TGG - tig0018115:18300-18319 Msa1364260:intron 45.0%
TACCAAACCATCACAGCAGA+AGG + tig0018115:18305-18324 None:intergenic 45.0%
GTGCTTGAAATCGTAAGGGT+CGG + tig0018115:18596-18615 None:intergenic 45.0%
! ATGATTGTAGCCTTGAGTGG+TGG - tig0018115:18716-18735 Msa1364260:intron 45.0%
GAGCTAATTGTCTTGCTCTC+TGG - tig0018115:18745-18764 Msa1364260:intron 45.0%
TTTGCATGCCGCCATGTTTA+TGG - tig0018115:19180-19199 Msa1364260:intron 45.0%
TAGGAGCCTTCTACCTATGT+GGG - tig0018115:19524-19543 Msa1364260:intron 45.0%
AGGAATCCCACATAGGTAGA+AGG + tig0018115:19533-19552 None:intergenic 45.0%
!! TTGCTGCTGCATTGGCATTT+TGG - tig0018115:19554-19573 Msa1364260:intron 45.0%
!! TGCTGCTGCATTGGCATTTT+GGG - tig0018115:19555-19574 Msa1364260:intron 45.0%
AAGTGGGAGGAAAAGGACTT+TGG - tig0018115:19581-19600 Msa1364260:intron 45.0%
GGAGGAAAAGGACTTTGGAT+TGG - tig0018115:19586-19605 Msa1364260:intron 45.0%
! AACCGCGGTGCTAAATTTGA+AGG - tig0018115:20038-20057 Msa1364260:intron 45.0%
AGGCAATTAAGGCTAGACAG+AGG - tig0018115:20249-20268 Msa1364260:CDS 45.0%
GCGACAATTCCCCACTTTAT+TGG - tig0018115:20343-20362 Msa1364260:CDS 45.0%
GGGTGAAGAGAGAAGAGTGA+TGG - tig0018115:15315-15334 Msa1364260:intron 50.0%
CAGCTACCATAGGACCTGAT+AGG + tig0018115:15459-15478 None:intergenic 50.0%
GAAACCGGTGACACCAGTAA+AGG + tig0018115:15575-15594 None:intergenic 50.0%
GCTTACCAGGAAGCTGAAAC+CGG + tig0018115:15590-15609 None:intergenic 50.0%
CACAAGTATTTCCCCACGCA+GGG + tig0018115:16025-16044 None:intergenic 50.0%
ACACAAGTATTTCCCCACGC+AGG + tig0018115:16026-16045 None:intergenic 50.0%
TTGCATCTGGGTCACACAAC+GGG + tig0018115:16189-16208 None:intergenic 50.0%
GTGGCCCAGATACAATGGTA+CGG - tig0018115:16224-16243 Msa1364260:intron 50.0%
AACTCATGAAGAGGGCCATG+AGG - tig0018115:16396-16415 Msa1364260:intron 50.0%
ATGAAGAGGGCCATGAGGTT+TGG - tig0018115:16401-16420 Msa1364260:CDS 50.0%
ATAATGGCGGCCAAACCTCA+TGG + tig0018115:16414-16433 None:intergenic 50.0%
TTGTGTGGGATGTGCCTTCA+AGG - tig0018115:16937-16956 Msa1364260:CDS 50.0%
!!! TGTGGCGAGTCCACTTTTTG+TGG - tig0018115:17102-17121 Msa1364260:intron 50.0%
!!! CGCTCTAGTTTTTGCGCATG+AGG + tig0018115:18228-18247 None:intergenic 50.0%
CGCTATACCTTCTGCTGTGA+TGG - tig0018115:18295-18314 Msa1364260:intron 50.0%
GCTCATATGACCACCACTCA+AGG + tig0018115:18729-18748 None:intergenic 50.0%
GCTGCAGCACCAATTCCAAA+TGG + tig0018115:18943-18962 None:intergenic 50.0%
!! TTGGAATTGGTGCTGCAGCA+AGG - tig0018115:18944-18963 Msa1364260:intron 50.0%
ACGGCAACACGTGCTTCAAA+TGG + tig0018115:19105-19124 None:intergenic 50.0%
AACATGGCGGCATGCAAAGA+GGG + tig0018115:19178-19197 None:intergenic 50.0%
AAACATGGCGGCATGCAAAG+AGG + tig0018115:19179-19198 None:intergenic 50.0%
CTAGGAGCCTTCTACCTATG+TGG - tig0018115:19523-19542 Msa1364260:intron 50.0%
CAGCAACAGGAATCCCACAT+AGG + tig0018115:19540-19559 None:intergenic 50.0%
GATTCCTGTTGCTGCTGCAT+TGG - tig0018115:19546-19565 Msa1364260:intron 50.0%
AATGCCAATGCAGCAGCAAC+AGG + tig0018115:19553-19572 None:intergenic 50.0%
!!! GCATTTTGGGTTCAAGTGGG+AGG - tig0018115:19568-19587 Msa1364260:intron 50.0%
TGGGTTCAAGTGGGAGGAAA+AGG - tig0018115:19574-19593 Msa1364260:intron 50.0%
!! TGTCACCGGTTTCAGCTTCC+TGG - tig0018115:15582-15601 Msa1364260:intron 55.0%
GTTGTGTGGCCCAGATGCAA+TGG - tig0018115:16103-16122 Msa1364260:CDS 55.0%
GTGACCCAGATGCAATGGCA+CGG - tig0018115:16138-16157 Msa1364260:CDS 55.0%
! GTCACCGTGCCATTGCATCT+GGG + tig0018115:16145-16164 None:intergenic 55.0%
! TGTCACCGTGCCATTGCATC+TGG + tig0018115:16146-16165 None:intergenic 55.0%
TCACACAACGGGTGGCACTT+TGG + tig0018115:16178-16197 None:intergenic 55.0%
CACGTATGTGGGTGGCACTT+TGG + tig0018115:16264-16283 None:intergenic 55.0%
AGGACTCTCACGTATGTGGG+TGG + tig0018115:16272-16291 None:intergenic 55.0%
TAGGTCGTGTTGGGTCACGA+GGG + tig0018115:16632-16651 None:intergenic 55.0%
! AGCATACAAGGCGTTCTCGC+AGG - tig0018115:19285-19304 Msa1364260:intron 55.0%
TCAGGGGTTGCTAGAGGTTG+TGG - tig0018115:19472-19491 Msa1364260:intron 55.0%
CAGGGGTTGCTAGAGGTTGT+GGG - tig0018115:19473-19492 Msa1364260:intron 55.0%
GGTTGTGGGTGGCAACACAT+AGG - tig0018115:19487-19506 Msa1364260:intron 55.0%
CATCTGGGTCACACAACGGG+TGG + tig0018115:16186-16205 None:intergenic 60.0%
AGGTCGTGTTGGGTCACGAG+GGG + tig0018115:16631-16650 None:intergenic 60.0%
GTAGGTCGTGTTGGGTCACG+AGG + tig0018115:16633-16652 None:intergenic 60.0%
CGTCACTGTCTTGGCTCCTC+TGG - tig0018115:19239-19258 Msa1364260:intron 60.0%
GGGTTGCTAGAGGTTGTGGG+TGG - tig0018115:19476-19495 Msa1364260:intron 60.0%
GTCGTGTTGGGTCACGAGGG+GGG + tig0018115:16629-16648 None:intergenic 65.0%
GGTCGTGTTGGGTCACGAGG+GGG + tig0018115:16630-16649 None:intergenic 65.0%
TTGGGTCACGAGGGGGGCAG+AGG + tig0018115:16623-16642 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0018115 gene 15224 20480 15224 ID=Msa1364260;Name=Msa1364260
tig0018115 mRNA 15224 20480 15224 ID=Msa1364260-mRNA-1;Parent=Msa1364260;Name=Msa1364260-mRNA-1;_AED=0.11;_eAED=0.11;_QI=68|1|1|1|0.75|0.55|9|139|495
tig0018115 exon 15224 15313 15224 ID=Msa1364260-mRNA-1:exon:13945;Parent=Msa1364260-mRNA-1
tig0018115 exon 15342 15453 15342 ID=Msa1364260-mRNA-1:exon:13944;Parent=Msa1364260-mRNA-1
tig0018115 exon 16023 16228 16023 ID=Msa1364260-mRNA-1:exon:13943;Parent=Msa1364260-mRNA-1
tig0018115 exon 16398 16636 16398 ID=Msa1364260-mRNA-1:exon:13942;Parent=Msa1364260-mRNA-1
tig0018115 exon 16739 16795 16739 ID=Msa1364260-mRNA-1:exon:13941;Parent=Msa1364260-mRNA-1
tig0018115 exon 16892 16978 16892 ID=Msa1364260-mRNA-1:exon:13940;Parent=Msa1364260-mRNA-1
tig0018115 exon 17383 17927 17383 ID=Msa1364260-mRNA-1:exon:13939;Parent=Msa1364260-mRNA-1
tig0018115 exon 20101 20401 20101 ID=Msa1364260-mRNA-1:exon:13938;Parent=Msa1364260-mRNA-1
tig0018115 exon 20423 20480 20423 ID=Msa1364260-mRNA-1:exon:13937;Parent=Msa1364260-mRNA-1
tig0018115 five_prime_UTR 20423 20480 20423 ID=Msa1364260-mRNA-1:five_prime_utr;Parent=Msa1364260-mRNA-1
tig0018115 five_prime_UTR 20392 20401 20392 ID=Msa1364260-mRNA-1:five_prime_utr;Parent=Msa1364260-mRNA-1
tig0018115 CDS 20101 20391 20101 ID=Msa1364260-mRNA-1:cds;Parent=Msa1364260-mRNA-1
tig0018115 CDS 17383 17927 17383 ID=Msa1364260-mRNA-1:cds;Parent=Msa1364260-mRNA-1
tig0018115 CDS 16892 16978 16892 ID=Msa1364260-mRNA-1:cds;Parent=Msa1364260-mRNA-1
tig0018115 CDS 16739 16795 16739 ID=Msa1364260-mRNA-1:cds;Parent=Msa1364260-mRNA-1
tig0018115 CDS 16398 16636 16398 ID=Msa1364260-mRNA-1:cds;Parent=Msa1364260-mRNA-1
tig0018115 CDS 16023 16228 16023 ID=Msa1364260-mRNA-1:cds;Parent=Msa1364260-mRNA-1
tig0018115 CDS 15391 15453 15391 ID=Msa1364260-mRNA-1:cds;Parent=Msa1364260-mRNA-1
tig0018115 three_prime_UTR 15342 15390 15342 ID=Msa1364260-mRNA-1:three_prime_utr;Parent=Msa1364260-mRNA-1
tig0018115 three_prime_UTR 15224 15313 15224 ID=Msa1364260-mRNA-1:three_prime_utr;Parent=Msa1364260-mRNA-1
Gene Sequence

>Msa1364260

ATGGGTGAAGAGAGAAGAGTGATGGAAGAGAGTCTCTTATCAAAACAAATTGATTCGAAAGCAGAGAATGACAATGAAGAAGAGAGAAAGAATAGAGAAATTAGTTGGGATATTTACACGAAAGAATTGAAGAGGATTTGTTACCTATCAGGTCCTATGGTAGCTGTAACATCTTCACAATATCTTTTACAGGTTGTGTCCATCATGATTGTTGGTCACTTAGGTGAACTCTATCTCTCTAGTGCCGCCTTAGCCATTTCCTTTACTGGTGTCACCGGTTTCAGCTTCCTGATGGGAATGGCTAGTGGACTAGAAACAACATGTGGACAGGCTTATGGAGCTAAACAATATCAAAGAATAGGAGTACAAACATACACATCTATATTTTCTCTCATATTGGTTTGTTTACCACTCTCTTTTATTTGGATCAACATTGAGAACATACTAGTTTTCACAGGACAAGACCCTCTAATTGCACATGAAGCAGGAAGATTCACAATTTGGCTTCTTCCAGCACTTTTTGCATATGCAATATTGCAACCACTAGTTAGATATTTTCAAATACAAAGCTTGCTTATTCCAATGCTTTTAAGCTCTTGTGTCACTCTTTGTATCCATATACCTCTTTGCTGGGCTTTGGTATTCAAGACAGGATTGAGTAATATTGGTGGTGCAATTGCAATGAGCATTTCCATTTGGTTAAATGTGATTTTTCTTGGATTATACATGAGATACTCTTCCTCATGCGCAAAAACTAGAGCGCCAATTTCTATGGAACTATTTCAAGGAATTTGGGAGTTTTTTCGCTTCGCTATACCTTCTGCTGTGATGGTTTGCCTTGAGTGGTGGTCATATGAGCTAATTGTCTTGCTCTCTGGACTTTTACCTAATCCACAACTTGAAACTTCAGTTCTATCAGTTTGTCTCAATACCATTGCAACTCTCTATACAATACCATTTGGAATTGGTGCTGCAGCAAGTACAAGGGTTTCAAATGAATTAGGAGCTGGTAATCCATTTGAAGCACGTGTTGCCGTGTTAGCTGCTATGTCACTTGCACTCACTGAAGCAAGTATAGTGAGCGCAACCCTCTTTGCATGCCGCCATGTTTATGGCTACATTTTCAGTAGTGATACGGAAGTTGTTAAATACGTCACTGTCTTGGCTCCTCTGGTTTCTATATCTGTTATACTCGACAGCATACAAGGCGTTCTCGCAGGGGTTGCTAGAGGTTGTGGGTGGCAACACATAGGAGTTTATGTGAATCTAGGAGCCTTCTACCTATGTGGGATTCCTGTTGCTGCTGCATTGGCATTTTGGGTTCAAGTGGGAGGAAAAGGACTTTGGATTGGTATACAAGTCGGTGCTTTTGTTCAATGTATTCTACTTTCTATCATAACATGTTGTATAAATTGGGAGCAACTGGCAATTAAGGCTAGACAGAGGTTGTTTGATGTTCAATTTTCTGGAGAGAATAGATTGGTATGA
Protein sequence

>Msa1364260

MGEERRVMEESLLSKQIDSKAENDNEEERKNREISWDIYTKELKRICYLSGPMVAVTSSQYLLQVVSIMIVGHLGELYLSSAALAISFTGVTGFSFLMGMASGLETTCGQAYGAKQYQRIGVQTYTSIFSLILVCLPLSFIWINIENILVFTGQDPLIAHEAGRFTIWLLPALFAYAILQPLVRYFQIQSLLIPMLLSSCVTLCIHIPLCWALVFKTGLSNIGGAIAMSISIWLNVIFLGLYMRYSSSCAKTRAPISMELFQGIWEFFRFAIPSAVMVCLEWWSYELIVLLSGLLPNPQLETSVLSVCLNTIATLYTIPFGIGAAASTRVSNELGAGNPFEARVAVLAAMSLALTEASIVSATLFACRHVYGYIFSSDTEVVKYVTVLAPLVSISVILDSIQGVLAGVARGCGWQHIGVYVNLGAFYLCGIPVAAALAFWVQVGGKGLWIGIQVGAFVQCILLSIITCCINWEQLAIKARQRLFDVQFSGENRLV*