AlfalfaGEDB | Msa0744540

M. sativa cultivar zhongmu-4 / Msa0744540

KEGG: K08243|K22374
GO: -
PFAM: PF00248.24

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0744540	AAB41556.1	99.359	312	2	0	1	312	1	312	0.0	641

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0744540	sp\|P26690\|6DCS_SOYBN	84.566	311	48	0	2	312	3	313	0.0	560

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0744540	Q40310	99.359	312	2	0	1	312	1	312	0.0	641

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0000610	Msa0744540	-0.806183	9.309480e-50	-8.615850e-47
Msa0000800	Msa0744540	0.815461	9.136903e-52	-8.615850e-47
Msa0005500	Msa0744540	-0.815766	7.814354e-52	-8.615850e-47
Msa0011060	Msa0744540	-0.817778	2.764431e-52	-8.615850e-47
Msa0011920	Msa0744540	-0.806984	6.306616e-50	-8.615850e-47
Msa0024490	Msa0744540	-0.814413	1.560304e-51	-8.615850e-47
Msa0025060	Msa0744540	-0.800434	1.444015e-48	-8.615850e-47
Msa0026420	Msa0744540	0.837141	6.191454e-57	-8.615850e-47
Msa0030050	Msa0744540	0.814742	1.319423e-51	-8.615850e-47
Msa0037280	Msa0744540	0.832426	9.517300e-56	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0744540	MtrunA17_Chr5g0447941	98.397	312	5	0	1	312	1	312	0.0	637
Msa0744540	MtrunA17_Chr5g0447951	98.077	312	6	0	1	312	1	312	0.0	636
Msa0744540	MtrunA17_Chr5g0447961	98.077	312	6	0	1	312	1	312	0.0	636
Msa0744540	MtrunA17_Chr3g0121001	68.182	308	97	1	6	312	7	314	5.74e-162	453
Msa0744540	MtrunA17_Chr1g0173621	62.951	305	110	3	1	302	1	305	1.22e-137	392
Msa0744540	MtrunA17_Chr6g0479981	50.165	303	144	5	1	300	1	299	3.91e-96	286
Msa0744540	MtrunA17_Chr6g0480011	49.186	307	143	8	1	300	1	301	2.46e-94	282
Msa0744540	MtrunA17_Chr6g0480021	48.366	306	124	7	19	311	16	300	2.79e-92	276
Msa0744540	MtrunA17_Chr8g0368921	42.958	284	159	2	19	300	15	297	2.41e-75	233
Msa0744540	MtrunA17_Chr4g0018921	41.754	285	161	4	18	300	12	293	1.38e-73	229

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0744540	AT1G59960.1	53.571	308	132	6	1	300	1	305	7.12e-112	327
Msa0744540	AT1G59950.1	55.903	288	117	5	20	300	15	299	1.25e-104	308
Msa0744540	AT2G37770.2	44.755	286	147	4	19	300	15	293	1.18e-77	239
Msa0744540	AT2G37790.1	43.357	286	151	4	19	300	15	293	2.17e-76	236
Msa0744540	AT5G62420.1	43.662	284	154	4	20	300	14	294	1.13e-73	229
Msa0744540	AT5G01670.1	42.254	284	155	6	19	300	23	299	1.14e-72	227
Msa0744540	AT2G37760.4	43.662	284	147	4	19	299	15	288	4.25e-72	224
Msa0744540	AT2G37760.3	43.662	284	147	4	19	299	15	288	4.25e-72	224
Msa0744540	AT2G37760.5	44.128	281	144	4	19	296	15	285	3.04e-71	223
Msa0744540	AT2G37760.2	43.617	282	146	4	19	297	15	286	2.42e-70	221

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Find 72 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTTGTGTATCTTTGAT+TGG	0.361005	5_1:-65385256	Msa0744540:CDS
AAAGAGATTGCAGATGCTCA+TGG	0.540234	5_1:-65385368	Msa0744540:CDS
AAAGTTTAGAAGTAACAAAA+AGG	0.467615	5_1:+65386152	None:intergenic
AAATTGATTATTTAAAACAA+CGG	0.602105	5_1:+65385035	None:intergenic
AACACTACCCATGTTGTTGT+TGG	0.397673	5_1:+65386405	None:intergenic
AAGAGATCTGCCACATCAAT+TGG	0.378807	5_1:+65385767	None:intergenic
AAGATGCCTGTAGTTGGAAT+GGG	0.562876	5_1:-65386340	Msa0744540:CDS
AAGCAAAATCGTTTGATCCC+TGG	0.455139	5_1:-65385194	Msa0744540:CDS
AAGTTTCAAGCTTTCCTCCA+TGG	0.483539	5_1:+65385715	None:intergenic
AATACTACTATGAAATAACT+TGG	0.542284	5_1:+65384997	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ATTGGAAATGAAAACTTTCC+AGG	+	chr5_1:65385659-65385678	None:intergenic	30.0%
ATTGGAAATGAAAACTTTCC+AGG	+	chr5_1:65385659-65385678	None:intergenic	30.0%
TTCTCAATCCAACAACAACA+TGG	-	chr5_1:65385028-65385047	Msa0744540:three_prime_UTR	35.0%
TCTCAATCCAACAACAACAT+GGG	-	chr5_1:65385029-65385048	Msa0744540:three_prime_UTR	35.0%
GTGTTTGTAAGAACCTTTGT+TGG	+	chr5_1:65385068-65385087	None:intergenic	35.0%
TTTCTTACATGTGAAGTCAG+GGG	+	chr5_1:65385132-65385151	None:intergenic	35.0%
CTTTCTTACATGTGAAGTCA+GGG	+	chr5_1:65385133-65385152	None:intergenic	35.0%
TCTTTCTTACATGTGAAGTC+AGG	+	chr5_1:65385134-65385153	None:intergenic	35.0%
ATCATTGAAGCCATCAAACA+AGG	-	chr5_1:65385167-65385186	Msa0744540:CDS	35.0%
TTGAAAGAGGCAATTGAACT+TGG	-	chr5_1:65385248-65385267	Msa0744540:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5_1	gene	65384990	65386473	65384990	ID=Msa0744540;Name=Msa0744540
chr5_1	mRNA	65384990	65386473	65384990	ID=Msa0744540-mRNA-1;Parent=Msa0744540;Name=Msa0744540-mRNA-1;_AED=0.15;_eAED=0.15;_QI=57\|1\|1\|1\|1\|1\|3\|164\|312
chr5_1	exon	65384990	65385528	65384990	ID=Msa0744540-mRNA-1:exon:19468;Parent=Msa0744540-mRNA-1
chr5_1	exon	65385622	65385865	65385622	ID=Msa0744540-mRNA-1:exon:19467;Parent=Msa0744540-mRNA-1
chr5_1	exon	65386097	65386473	65386097	ID=Msa0744540-mRNA-1:exon:19466;Parent=Msa0744540-mRNA-1
chr5_1	five_prime_UTR	65386417	65386473	65386417	ID=Msa0744540-mRNA-1:five_prime_utr;Parent=Msa0744540-mRNA-1
chr5_1	CDS	65386097	65386416	65386097	ID=Msa0744540-mRNA-1:cds;Parent=Msa0744540-mRNA-1
chr5_1	CDS	65385622	65385865	65385622	ID=Msa0744540-mRNA-1:cds;Parent=Msa0744540-mRNA-1
chr5_1	CDS	65385154	65385528	65385154	ID=Msa0744540-mRNA-1:cds;Parent=Msa0744540-mRNA-1
chr5_1	three_prime_UTR	65384990	65385153	65384990	ID=Msa0744540-mRNA-1:three_prime_utr;Parent=Msa0744540-mRNA-1

Gene Sequence

>Msa0744540

ATGGGTAGTGTTGAAATTCCAACAAAGGTTCTTACAAACACATCTAGTCAATTGAAGATGCCTGTAGTTGGAATGGGATCAGCCCCTGACTTCACATGTAAGAAAGACACAAAAGATGCAATCATTGAAGCCATCAAACAAGGTTATAGACACTTTGATACAGCTGCTGCATATGGCTCAGAACAAGCTCTTGGAGAGGCTTTGAAAGAGGCAATTGAACTTGGTCTTGTCACTAGAGAAGACCTTTTTGTTACTTCTAAACTTTGGGTTACTGAAAACCATCCTCATCTTGTTATTCCTGCTCTTCAAAAATCTCTCAAGACTCTTCAACTAGACTACTTGGACTTGTACTTGATTCATTGGCCACTTAGTTCTCAGCCTGGAAAGTTTTCATTTCCAATTGATGTGGCAGATCTCTTACCATTTGATGTCAAAGGTGTTTGGGAATCCATGGAGGAAAGCTTGAAACTTGGACTCACTAAAGCTATTGGAGTTAGTAACTTCTCTGTCAAGAAACTTGAAAATCTTCTCTCTGTTGCCACTGTTCTTCCTGCAGTCAATCAAGTGGAGATGAACCTTGCATGGCAACAAAAGAAGCTTAGAGAGTTTTGCAACGCACACGGAATAGTGTTAACCGCATTTTCCCCGTTGAGGAAAGGTGCAAGCAGAGGACCTAATGAGGTTATGGAGAATGATATGCTTAAAGAGATTGCAGATGCTCATGGAAAGTCTGTTGCACAAATATCTCTAAGATGGTTATACGAACAAGGAGTCACTTTTGTTCCCAAGAGCTACGATAAGGAGAGAATGAACCAAAATTTGTGTATCTTTGATTGGTCATTGACAAAGGAGGATCATGAGAAGATTGATCAAATTAAGCAAAATCGTTTGATCCCTGGACCAACCAAGCCCGGACTCAATGACCTCTATGATGACTAA

Protein sequence

>Msa0744540

MGSVEIPTKVLTNTSSQLKMPVVGMGSAPDFTCKKDTKDAIIEAIKQGYRHFDTAAAYGSEQALGEALKEAIELGLVTREDLFVTSKLWVTENHPHLVIPALQKSLKTLQLDYLDLYLIHWPLSSQPGKFSFPIDVADLLPFDVKGVWESMEESLKLGLTKAIGVSNFSVKKLENLLSVATVLPAVNQVEMNLAWQQKKLREFCNAHGIVLTAFSPLRKGASRGPNEVMENDMLKEIADAHGKSVAQISLRWLYEQGVTFVPKSYDKERMNQNLCIFDWSLTKEDHEKIDQIKQNRLIPGPTKPGLNDLYDD*