AlfalfaGEDB | Msa1364360

M. sativa cultivar zhongmu-4 / Msa1364360

KEGG: -
GO: -
PFAM: PF02701.18

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1364360	RHN44270.1	89.117	487	42	3	1	477	98	583	0.0	870

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1364360	sp\|Q93ZL5\|CDF2_ARATH	41.051	514	202	19	6	477	3	457	1.04e-88	281

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1364360	A0A396GT85	89.117	487	42	3	1	477	98	583	0.0	870

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TFs/TRs:

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Gene ID	Type	Classification
Msa1364360	TF	C2C2-Dof

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1048950	Msa1364360	0.911392	6.402886e-83	-8.615850e-47
Msa1100950	Msa1364360	0.989661	4.329434e-179	-8.615850e-47
Msa1364360	Msa1437140	0.932347	9.763620e-95	-8.615850e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1364360	MtrunA17_Chr7g0217581	89.117	487	42	3	1	477	98	583	0.0	870
Msa1364360	MtrunA17_Chr6g0453321	56.313	499	192	10	1	477	1	495	4.69e-170	488
Msa1364360	MtrunA17_Chr4g0042481	42.857	497	228	12	1	475	1	463	9.60e-112	338
Msa1364360	MtrunA17_Chr3g0092111	39.847	522	212	19	1	477	1	465	3.18e-89	280
Msa1364360	MtrunA17_Chr5g0417601	37.654	486	181	15	4	475	2	379	8.51e-70	227
Msa1364360	MtrunA17_Chr8g0355261	34.888	493	239	21	3	476	9	438	1.50e-66	220
Msa1364360	MtrunA17_Chr5g0417561	35.579	475	188	18	4	475	2	361	8.76e-66	216
Msa1364360	MtrunA17_Chr5g0417551	35.041	488	181	20	4	475	2	369	5.31e-62	206
Msa1364360	MtrunA17_Chr7g0254161	34.651	430	195	14	78	476	49	423	2.84e-50	177
Msa1364360	MtrunA17_Chr5g0417571	41.434	251	119	7	6	238	4	244	2.72e-40	147

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1364360	AT5G39660.3	41.051	514	202	19	6	477	3	457	1.06e-89	281
Msa1364360	AT5G39660.1	41.051	514	202	19	6	477	3	457	1.06e-89	281
Msa1364360	AT5G39660.2	41.051	514	202	19	6	477	3	457	1.06e-89	281
Msa1364360	AT3G47500.1	41.093	494	223	23	1	475	2	446	1.56e-81	260
Msa1364360	AT5G62430.1	38.095	378	122	16	108	475	21	296	3.09e-55	186
Msa1364360	AT1G69570.1	33.065	496	212	20	1	476	1	396	2.43e-50	176
Msa1364360	AT1G26790.1	31.263	499	186	24	1	476	7	371	2.00e-38	144
Msa1364360	AT1G29160.1	85.000	60	9	0	130	189	56	115	5.66e-35	129
Msa1364360	AT2G34140.1	81.667	60	11	0	130	189	52	111	4.43e-33	123
Msa1364360	AT5G60200.1	58.333	72	30	0	132	203	51	122	1.97e-25	105

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Find 129 sgRNAs with CRISPR-Local

Find 210 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGCCAAGGAGTTCCATG+AGG	0.739950	tig0018118:+9439	None:intergenic
AACATGCTAAAATCAAGCGA+AGG	0.639475	tig0018118:-9202	Msa1364360:CDS
AACCTTAGGTAAACATTCAA+GGG	0.580141	tig0018118:-9230	Msa1364360:CDS
AACGGTACAATCCTTACATT+TGG	0.421611	tig0018118:-9724	Msa1364360:CDS
AAGCGACACTTCAGAATTCT+CGG	0.433945	tig0018118:-9768	Msa1364360:CDS
AAGGATAAGAATAAGAATGA+GGG	0.506790	tig0018118:-8600	Msa1364360:three_prime_UTR
AATCCTGATGGACAAAAGGC+CGG	0.603585	tig0018118:+9376	None:intergenic
AATGCCTGAAAGAGTCTTCG+CGG	0.584851	tig0018118:+9040	None:intergenic
AATGGCATGGAAAATCCTGA+TGG	0.590674	tig0018118:+9364	None:intergenic
ACAAGAACATGAGATTGAGA+AGG	0.637128	tig0018118:-11452	Msa1364360:intron

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CCAATTTCAGAAATTCATGT+TGG	-	tig0018118:8599-8618	Msa1364360:three_prime_UTR	30.0%
AGAATTAGGAAGCTATTGAA+AGG	-	tig0018118:9239-9258	Msa1364360:CDS	30.0%
GTTCAACAAAATCACAATCA+CGG	-	tig0018118:9320-9339	Msa1364360:CDS	30.0%
CACAAACTGATACAAAAACA+AGG	-	tig0018118:9649-9668	Msa1364360:CDS	30.0%
TCGAATAAAAAGAACGAAGA+TGG	-	tig0018118:10017-10036	Msa1364360:CDS	30.0%
GTGCAAAATTACACAAGAAA+TGG	-	tig0018118:10518-10537	Msa1364360:intron	30.0%
GGATCAATCAAATAAATCGT+CGG	-	tig0018118:10601-10620	Msa1364360:intron	30.0%
ATTCTATCCTGCAACTTATT+GGG	-	tig0018118:10817-10836	Msa1364360:intron	30.0%
AACCTTAGGTAAACATTCAA+GGG	-	tig0018118:10931-10950	Msa1364360:intron	30.0%
TATATACATCTAAGAGGTCT+TGG	+	tig0018118:11538-11557	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0018118	gene	8451	11732	8451	ID=Msa1364360;Name=Msa1364360
tig0018118	mRNA	8451	11732	8451	ID=Msa1364360-mRNA-1;Parent=Msa1364360;Name=Msa1364360-mRNA-1;_AED=0.01;_eAED=0.01;_QI=100\|1\|1\|1\|1\|1\|2\|483\|477
tig0018118	exon	8451	10187	8451	ID=Msa1364360-mRNA-1:exon:13980;Parent=Msa1364360-mRNA-1
tig0018118	exon	11453	11732	11453	ID=Msa1364360-mRNA-1:exon:13979;Parent=Msa1364360-mRNA-1
tig0018118	five_prime_UTR	11633	11732	11633	ID=Msa1364360-mRNA-1:five_prime_utr;Parent=Msa1364360-mRNA-1
tig0018118	CDS	11453	11632	11453	ID=Msa1364360-mRNA-1:cds;Parent=Msa1364360-mRNA-1
tig0018118	CDS	8934	10187	8934	ID=Msa1364360-mRNA-1:cds;Parent=Msa1364360-mRNA-1
tig0018118	three_prime_UTR	8451	8933	8451	ID=Msa1364360-mRNA-1:three_prime_utr;Parent=Msa1364360-mRNA-1

Gene Sequence

>Msa1364360

ATGTTGGAGACAAAAGACTCTGCGATCAAACTGTTTGGAAAAACAATTCCAATTTCAGAAATTCATGTTGGAAAAACAATTTCAGAGAGTATTGATGATCATCATCATGATTTTTCTACAAACTCTTCAAATGAGTCTCATAATAATAAAGATGCTTCACAAGAACATGAGATTGAGAAGAACACAACGGGAGAAAAACTGTCGAATAAAAAGAACGAAGATGGATTCCTTGCACAATCAACAGAAGAATTCACTAATCCAGATGCAACTTCTAGAACTGATGAAGAATCTGTTACAGTATCCACCGAAAGGGAGGTTGCTACATTGAAAACTTCAAAGACCGAAGAAGAACAGGACGAGACAAGTAACACACAGGATAAGCACCTCAAAAAACCGGACAAAATAGTTCCTTGTCCTCGTTGTAGTAGCATGGACACGAAGTTCTGCTACTATAATAATTACAATGTCAACCAACCCCGTCATTTCTGCAAGAACTGCCAGAGATATTGGACTGCCGGTGGAACCATGAGAAACGTACCAGTAGGTGCCGGTCGCAGAAAAAACAAGAGCTCTTCAGAAGCGACACTTCAGAATTCTCGGATACACCCTTCCTTGAAATGCAACGGTACAATCCTTACATTTGGATCCAACTCACCTTTATGTGATTCAGTGGCATCTGTTTTGAAACTTGCTGATAAAACAGTGCAAAATTACACAAGAAATGGATATCATAAACATGAAGAACTAAGAATTTGTGTTCCTCATACCAGTGAAGAACAAGGGGAGGATCAATCAAATAAATCGTCGGTTACCTCCACAAAGTCGACGGAAGGTGCAACCACCAATGTATCTCAAGAACAAGCTATGTGGAATGGCCATAGCTTCCCACCACAAGGTGGCTATTTTCCTCATGGAACTCCTTGGCATTTACCATGGAATCCGATGCAAACGAGCTCACTGATTCCACCACCGGCCTTTTGTCCATCAGGATTTTCCATGCCATTCTATCCTGCAACTTATTGGGGTTGTACTATGCCAAGTGCATGGAACATTCCACGGCAAGCACAACCATCATCACCGAATGGTGCAAACCATAACTCTACTACAAACTCTCCAACCTTAGGTAAACATTCAAGGGAGGAAAACATGCTAAAATCAAGCGAAGGAGATGGTAAGAAAGAAATAAGCGAAGAAAAGAGCTTGTGGTTTCCTAAAACTTTGAGGATCGATGACTCAGAGGAAGCAGAAAAAAGCTCTTTCTGGACAACCCTTGGGATTAAAAACAACAACGCTGACTCAGTTCCTCCGCGAAGACTCTTTCAGGCATTCCCTTCAAAGTGCGACGAGAAAAATCACTCGGTACAAGTGTCTTCAGTCTTGCAAGCCAATCCGGCGGCATTGTCTAGGTCGCTTCACTTTCACGAGACTTCATAA

Protein sequence

>Msa1364360

MLETKDSAIKLFGKTIPISEIHVGKTISESIDDHHHDFSTNSSNESHNNKDASQEHEIEKNTTGEKLSNKKNEDGFLAQSTEEFTNPDATSRTDEESVTVSTEREVATLKTSKTEEEQDETSNTQDKHLKKPDKIVPCPRCSSMDTKFCYYNNYNVNQPRHFCKNCQRYWTAGGTMRNVPVGAGRRKNKSSSEATLQNSRIHPSLKCNGTILTFGSNSPLCDSVASVLKLADKTVQNYTRNGYHKHEELRICVPHTSEEQGEDQSNKSSVTSTKSTEGATTNVSQEQAMWNGHSFPPQGGYFPHGTPWHLPWNPMQTSSLIPPPAFCPSGFSMPFYPATYWGCTMPSAWNIPRQAQPSSPNGANHNSTTNSPTLGKHSREENMLKSSEGDGKKEISEEKSLWFPKTLRIDDSEEAEKSSFWTTLGIKNNNADSVPPRRLFQAFPSKCDEKNHSVQVSSVLQANPAALSRSLHFHETS*