Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1367380 | AES74278.2 | 88.889 | 90 | 10 | 0 | 4 | 93 | 1 | 90 | 6.82e-39 | 134 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1367380 | G7J8G9 | 88.889 | 90 | 10 | 0 | 4 | 93 | 1 | 90 | 3.26e-39 | 134 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1365050 | Msa1367380 | 0.828474 | 8.813535e-55 | -8.615850e-47 |
| Msa1367380 | Msa1457680 | 0.827605 | 1.426205e-54 | -8.615850e-47 |
| Msa0616510 | Msa1367380 | 0.810980 | 8.798243e-51 | -8.615850e-47 |
| Msa0699840 | Msa1367380 | 0.820307 | 7.349367e-53 | -8.615850e-47 |
| Msa0137210 | Msa1367380 | 0.808798 | 2.594435e-50 | -8.615850e-47 |
| Msa1341890 | Msa1367380 | 0.813917 | 2.007470e-51 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1367380 | MtrunA17_Chr3g0145471 | 88.889 | 90 | 10 | 0 | 4 | 93 | 1 | 90 | 6.27e-43 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCACTTGCTCATGGTATT+TGG | 0.221621 | tig0018513:-64178 | Msa1367380:CDS |
| GATAAGTAGTTGAAAGTTGA+TGG | 0.350576 | tig0018513:+64090 | None:intergenic |
| TTCTCAGTACAACCACTTCA+AGG | 0.423962 | tig0018513:-64131 | Msa1367380:CDS |
| ATACCATGAGCAAGTGGAAT+GGG | 0.424845 | tig0018513:+64182 | None:intergenic |
| AAGCTCAACTTTCACACGCT+TGG | 0.439936 | tig0018513:-64013 | Msa1367380:CDS |
| TTCATTGGTTCATATTCTAC+CGG | 0.459746 | tig0018513:-64040 | Msa1367380:CDS |
| TAAAACAAATTAGAATGGAG+TGG | 0.483489 | tig0018513:-64279 | None:intergenic |
| CTAAGAGAAGGGGTACAGAA+TGG | 0.530072 | tig0018513:-64249 | Msa1367380:CDS |
| GTGGGTGCAGTCTAAGAGAA+GGG | 0.558450 | tig0018513:-64260 | Msa1367380:CDS |
| AAAACAAATTAGAATGGAGT+GGG | 0.565648 | tig0018513:-64278 | None:intergenic |
| ACCATGAGCAAGTGGAATGG+GGG | 0.577759 | tig0018513:+64184 | None:intergenic |
| GTGAAAGTTGAGCTTTCCAC+CGG | 0.578373 | tig0018513:+64021 | None:intergenic |
| AATACCATGAGCAAGTGGAA+TGG | 0.583926 | tig0018513:+64181 | None:intergenic |
| TGTGTAACTGAGCCTTGAAG+TGG | 0.589242 | tig0018513:+64119 | None:intergenic |
| ATTGGTTCATATTCTACCGG+TGG | 0.591044 | tig0018513:-64037 | Msa1367380:CDS |
| TACCATGAGCAAGTGGAATG+GGG | 0.604366 | tig0018513:+64183 | None:intergenic |
| GAATGGAAACAAGGATGGAG+AGG | 0.605725 | tig0018513:-64232 | Msa1367380:CDS |
| AGTGGGTGCAGTCTAAGAGA+AGG | 0.606015 | tig0018513:-64261 | Msa1367380:CDS |
| TGCCAAATACCATGAGCAAG+TGG | 0.608145 | tig0018513:+64176 | None:intergenic |
| GTACAGAATGGAAACAAGGA+TGG | 0.619461 | tig0018513:-64237 | Msa1367380:CDS |
| GCTCATGGTATTTGGCATCG+TGG | 0.630406 | tig0018513:-64170 | Msa1367380:CDS |
| TGGGTGCAGTCTAAGAGAAG+GGG | 0.655616 | tig0018513:-64259 | Msa1367380:CDS |
| AGGGGTACAGAATGGAAACA+AGG | 0.689848 | tig0018513:-64241 | Msa1367380:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATGAAAAACATAAGCAAGAA+GGG | + | tig0018513:64216-64235 | None:intergenic | 25.0% |
| ! | AATGAAAAACATAAGCAAGA+AGG | + | tig0018513:64217-64236 | None:intergenic | 25.0% |
| !! | TTCTTGCTTATGTTTTTCAT+TGG | - | tig0018513:64216-64235 | Msa1367380:CDS | 25.0% |
| GATAAGTAGTTGAAAGTTGA+TGG | + | tig0018513:64184-64203 | None:intergenic | 30.0% | |
| TGAAAAACATAAGCAAGAAG+GGG | + | tig0018513:64215-64234 | None:intergenic | 30.0% | |
| !! | TTCATTGGTTCATATTCTAC+CGG | - | tig0018513:64231-64250 | Msa1367380:CDS | 30.0% |
| GTACAGAATGGAAACAAGGA+TGG | - | tig0018513:64034-64053 | Msa1367380:CDS | 40.0% | |
| ATACCATGAGCAAGTGGAAT+GGG | + | tig0018513:64092-64111 | None:intergenic | 40.0% | |
| AATACCATGAGCAAGTGGAA+TGG | + | tig0018513:64093-64112 | None:intergenic | 40.0% | |
| TTCCACTTGCTCATGGTATT+TGG | - | tig0018513:64093-64112 | Msa1367380:CDS | 40.0% | |
| TTCTCAGTACAACCACTTCA+AGG | - | tig0018513:64140-64159 | Msa1367380:CDS | 40.0% | |
| ! | ATTGGTTCATATTCTACCGG+TGG | - | tig0018513:64234-64253 | Msa1367380:CDS | 40.0% |
| ! | CTAAGAGAAGGGGTACAGAA+TGG | - | tig0018513:64022-64041 | Msa1367380:CDS | 45.0% |
| ! | AGGGGTACAGAATGGAAACA+AGG | - | tig0018513:64030-64049 | Msa1367380:CDS | 45.0% |
| GAATGGAAACAAGGATGGAG+AGG | - | tig0018513:64039-64058 | Msa1367380:CDS | 45.0% | |
| TACCATGAGCAAGTGGAATG+GGG | + | tig0018513:64091-64110 | None:intergenic | 45.0% | |
| TGCCAAATACCATGAGCAAG+TGG | + | tig0018513:64098-64117 | None:intergenic | 45.0% | |
| TGTGTAACTGAGCCTTGAAG+TGG | + | tig0018513:64155-64174 | None:intergenic | 45.0% | |
| GTGAAAGTTGAGCTTTCCAC+CGG | + | tig0018513:64253-64272 | None:intergenic | 45.0% | |
| AAGCTCAACTTTCACACGCT+TGG | - | tig0018513:64258-64277 | Msa1367380:CDS | 45.0% | |
| AGTGGGTGCAGTCTAAGAGA+AGG | - | tig0018513:64010-64029 | Msa1367380:CDS | 50.0% | |
| GTGGGTGCAGTCTAAGAGAA+GGG | - | tig0018513:64011-64030 | Msa1367380:CDS | 50.0% | |
| TGGGTGCAGTCTAAGAGAAG+GGG | - | tig0018513:64012-64031 | Msa1367380:CDS | 50.0% | |
| ACCATGAGCAAGTGGAATGG+GGG | + | tig0018513:64090-64109 | None:intergenic | 50.0% | |
| GCTCATGGTATTTGGCATCG+TGG | - | tig0018513:64101-64120 | Msa1367380:CDS | 50.0% | |
| ACCCCCATTCCACTTGCTCA+TGG | - | tig0018513:64086-64105 | Msa1367380:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0018513 | gene | 64006 | 64287 | 64006 | ID=Msa1367380;Name=Msa1367380 |
| tig0018513 | mRNA | 64006 | 64287 | 64006 | ID=Msa1367380-mRNA-1;Parent=Msa1367380;Name=Msa1367380-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|93 |
| tig0018513 | exon | 64006 | 64287 | 64006 | ID=Msa1367380-mRNA-1:exon:15520;Parent=Msa1367380-mRNA-1 |
| tig0018513 | CDS | 64006 | 64287 | 64006 | ID=Msa1367380-mRNA-1:cds;Parent=Msa1367380-mRNA-1 |
| Gene Sequence |
| Protein sequence |
