AlfalfaGEDB | Msa1365050

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1365050	AES74278.2	87.778	90	11	0	4	93	1	90	3.65e-38	132

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1365050	G7J8G9	87.778	90	11	0	4	93	1	90	1.74e-38	132

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa1365050	Msa1367380	0.828474	8.813535e-55	-8.615850e-47
Msa1365050	Msa1457680	0.994322	2.592729e-206	-8.615850e-47
Msa0616510	Msa1365050	0.808133	3.597664e-50	-8.615850e-47
Msa0699840	Msa1365050	0.822348	2.485525e-53	-8.615850e-47
Msa0137210	Msa1365050	0.800284	1.548993e-48	-8.615850e-47
Msa0182400	Msa1365050	0.802245	6.146669e-49	-8.615850e-47
Msa1341890	Msa1365050	0.812382	4.360614e-51	-8.615850e-47
Msa0562620	Msa1365050	0.816071	6.679376e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1365050	MtrunA17_Chr3g0145471	87.778	90	11	0	4	93	1	90	3.36e-42	132
Msa1365050	MtrunA17_Chr4g0038521	47.170	53	27	1	1	52	1	53	5.39e-11	53.1

Find 23 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCCACTTGCTCATGGTATT+TGG	0.221621	tig0018197:+104124	Msa1365050:CDS
GATAAGTAGTTGAAAGTTGA+TGG	0.350576	tig0018197:-104212	None:intergenic
TTCTCAGTACAACCACTTCA+AGG	0.423962	tig0018197:+104171	Msa1365050:CDS
ATACCATGAGCAAGTGGAAT+GGG	0.424845	tig0018197:-104120	None:intergenic
AAGCTCAACTTTCACACGCT+TGG	0.439936	tig0018197:+104289	Msa1365050:CDS
TTCATTGGTTCATATTCTAC+CGG	0.459746	tig0018197:+104262	Msa1365050:CDS
TAAAACAAATTAGAATGGAG+TGG	0.483489	tig0018197:+104023	None:intergenic
CTAAGAGAAGGGGTGCAGAA+TGG	0.557799	tig0018197:+104053	Msa1365050:CDS
GTGGGTGCAGTCTAAGAGAA+GGG	0.558450	tig0018197:+104042	Msa1365050:CDS
AAAACAAATTAGAATGGAGT+GGG	0.565648	tig0018197:+104024	None:intergenic
ACCATGAGCAAGTGGAATGG+GGG	0.577759	tig0018197:-104118	None:intergenic
GTGAAAGTTGAGCTTTCCAC+CGG	0.578373	tig0018197:-104281	None:intergenic
AATACCATGAGCAAGTGGAA+TGG	0.583926	tig0018197:-104121	None:intergenic
TGTGTAACTGAGCCTTGAAG+TGG	0.589242	tig0018197:-104183	None:intergenic
ATTGGTTCATATTCTACCGG+TGG	0.591044	tig0018197:+104265	Msa1365050:CDS
GTGCAGAATGGAAACAAGGA+TGG	0.597122	tig0018197:+104065	Msa1365050:CDS
TACCATGAGCAAGTGGAATG+GGG	0.604366	tig0018197:-104119	None:intergenic
AGTGGGTGCAGTCTAAGAGA+AGG	0.606015	tig0018197:+104041	Msa1365050:CDS
TGCCAAATACCATGAGCAAG+TGG	0.608145	tig0018197:-104126	None:intergenic
GAATGGAAACAAGGATGGAG+AGG	0.628361	tig0018197:+104070	Msa1365050:CDS
GCTCATGGTATTTGGCATCG+TGG	0.630406	tig0018197:+104132	Msa1365050:CDS
TGGGTGCAGTCTAAGAGAAG+GGG	0.653177	tig0018197:+104043	Msa1365050:CDS
AGGGGTGCAGAATGGAAACA+AGG	0.663100	tig0018197:+104061	Msa1365050:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATGAAAAACATAAGCAAGAA+GGG	-	tig0018197:104247-104266	None:intergenic	25.0%
!	AATGAAAAACATAAGCAAGA+AGG	-	tig0018197:104248-104267	None:intergenic	25.0%
!!	TTCTTGCTTATGTTTTTCAT+TGG	+	tig0018197:104247-104266	Msa1365050:CDS	25.0%
	GATAAGTAGTTGAAAGTTGA+TGG	-	tig0018197:104215-104234	None:intergenic	30.0%
	TGAAAAACATAAGCAAGAAG+GGG	-	tig0018197:104246-104265	None:intergenic	30.0%
!!	TTCATTGGTTCATATTCTAC+CGG	+	tig0018197:104262-104281	Msa1365050:CDS	30.0%
	ATACCATGAGCAAGTGGAAT+GGG	-	tig0018197:104123-104142	None:intergenic	40.0%
	AATACCATGAGCAAGTGGAA+TGG	-	tig0018197:104124-104143	None:intergenic	40.0%
	TTCCACTTGCTCATGGTATT+TGG	+	tig0018197:104124-104143	Msa1365050:CDS	40.0%
	TTCTCAGTACAACCACTTCA+AGG	+	tig0018197:104171-104190	Msa1365050:CDS	40.0%
!	ATTGGTTCATATTCTACCGG+TGG	+	tig0018197:104265-104284	Msa1365050:CDS	40.0%
	GTGCAGAATGGAAACAAGGA+TGG	+	tig0018197:104065-104084	Msa1365050:CDS	45.0%
	GAATGGAAACAAGGATGGAG+AGG	+	tig0018197:104070-104089	Msa1365050:CDS	45.0%
	TACCATGAGCAAGTGGAATG+GGG	-	tig0018197:104122-104141	None:intergenic	45.0%
	TGCCAAATACCATGAGCAAG+TGG	-	tig0018197:104129-104148	None:intergenic	45.0%
	TGTGTAACTGAGCCTTGAAG+TGG	-	tig0018197:104186-104205	None:intergenic	45.0%
	GTGAAAGTTGAGCTTTCCAC+CGG	-	tig0018197:104284-104303	None:intergenic	45.0%
	AAGCTCAACTTTCACACGCT+TGG	+	tig0018197:104289-104308	Msa1365050:CDS	45.0%
	AGTGGGTGCAGTCTAAGAGA+AGG	+	tig0018197:104041-104060	Msa1365050:CDS	50.0%
	GTGGGTGCAGTCTAAGAGAA+GGG	+	tig0018197:104042-104061	Msa1365050:CDS	50.0%
	TGGGTGCAGTCTAAGAGAAG+GGG	+	tig0018197:104043-104062	Msa1365050:CDS	50.0%
!	CTAAGAGAAGGGGTGCAGAA+TGG	+	tig0018197:104053-104072	Msa1365050:CDS	50.0%
!	AGGGGTGCAGAATGGAAACA+AGG	+	tig0018197:104061-104080	Msa1365050:CDS	50.0%
	ACCATGAGCAAGTGGAATGG+GGG	-	tig0018197:104121-104140	None:intergenic	50.0%
	GCTCATGGTATTTGGCATCG+TGG	+	tig0018197:104132-104151	Msa1365050:CDS	50.0%
	ACCCCCATTCCACTTGCTCA+TGG	+	tig0018197:104117-104136	Msa1365050:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
tig0018197	gene	104037	104318	104037	ID=Msa1365050;Name=Msa1365050
tig0018197	mRNA	104037	104318	104037	ID=Msa1365050-mRNA-1;Parent=Msa1365050;Name=Msa1365050-mRNA-1;_AED=0.01;_eAED=0.01;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|93
tig0018197	exon	104037	104318	104037	ID=Msa1365050-mRNA-1:exon:14367;Parent=Msa1365050-mRNA-1
tig0018197	CDS	104037	104318	104037	ID=Msa1365050-mRNA-1:cds;Parent=Msa1365050-mRNA-1

Gene Sequence	>Msa1365050 ATGGAGTGGGTGCAGTCTAAGAGAAGGGGTGCAGAATGGAAACAAGGATGGAGAGGAAAGAGCATGACTTCTATTTCTGCACCCCCATTCCACTTGCTCATGGTATTTGGCATCGTGGTTAGCTTGTTGTGTTTTTCTCAGTACAACCACTTCAAGGCTCAGTTACACAACACTGCCATCAACTTTCAACTACTTATCTTCTTCTTCCCCTTCTTGCTTATGTTTTTCATTGGTTCATATTCTACCGGTGGAAAGCTCAACTTTCACACGCTTGGAGCATGA
Protein sequence	>Msa1365050 MEWVQSKRRGAEWKQGWRGKSMTSISAPPFHLLMVFGIVVSLLCFSQYNHFKAQLHNTAINFQLLIFFFPFLLMFFIGSYSTGGKLNFHTLGA*

M. sativa cultivar zhongmu-4 / Msa1365050