AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1367630

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1367630 MtrunA17_Chr5g0448691 96.316 380 8 3 3 379 2 378 0.0 733
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1367630 AT2G17265.1 69.867 375 103 4 4 376 2 368 1.13e-163 463
Msa1367630 AT4G35295.1 57.143 63 22 1 314 376 54 111 3.04e-13 66.2

Find 110 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGTTGGGGCTTCAAATTC+TGG 0.168994 tig0018533:-33542 None:intergenic
AAATCAGAAGAGAAGGTTTC+TGG 0.179307 tig0018533:+33392 Msa1367630:CDS
TCTTGTGGCGTCAGTGTTAC+AGG 0.194081 tig0018533:+33637 Msa1367630:CDS
GCCATTCAAGCTGGAGCTTT+TGG 0.204037 tig0018533:+33758 Msa1367630:CDS
TTACCTTTGGGAAGTGGTTT+GGG 0.235266 tig0018533:+33275 Msa1367630:CDS
TTTACCTTTGGGAAGTGGTT+TGG 0.257806 tig0018533:+33274 Msa1367630:CDS
ATATCCATCTCACATATTTC+TGG 0.261329 tig0018533:+33125 Msa1367630:CDS
ATCTCACCGGGGTGAACATC+TGG 0.273214 tig0018533:-33104 None:intergenic
CCTGCCACCGTCGCCAATCT+AGG 0.297972 tig0018533:+33014 Msa1367630:CDS
TGCAGCGGCGCTGGCAGCAC+TGG 0.313196 tig0018533:-33300 None:intergenic
TCTCGAAAAGGGTTTACCTT+TGG 0.330871 tig0018533:+33262 Msa1367630:CDS
TTACTGAGTTTGTGGATGTT+AGG 0.331672 tig0018533:-33155 None:intergenic
CTCGAAAAGGGTTTACCTTT+GGG 0.332798 tig0018533:+33263 Msa1367630:CDS
CTTAGGTTCAACAATCTTAT+CGG 0.352515 tig0018533:-33693 None:intergenic
GCAGCACTGCCTTTAGAGAT+AGG 0.366224 tig0018533:+33575 Msa1367630:CDS
TCTTTCACTTTCTCTCGAAA+AGG 0.371475 tig0018533:+33250 Msa1367630:CDS
TAGGGATGCCGCACCATGTC+TGG 0.375083 tig0018533:+33594 Msa1367630:CDS
AAGCGTGCACCATTGATTCC+TGG 0.387995 tig0018533:+33713 Msa1367630:CDS
GATTCGCAATTACCAACCTT+TGG 0.392630 tig0018533:+33463 Msa1367630:CDS
TGCCAGCGCCGCTGCAGCCG+CGG 0.400058 tig0018533:+33307 Msa1367630:CDS
AACCGCGGCTGCAGCGGCGC+TGG 0.405837 tig0018533:-33309 None:intergenic
GGCTGCACCATCAGTGGTGC+TGG 0.412382 tig0018533:+33779 Msa1367630:CDS
CCTAGATTGGCGACGGTGGC+AGG 0.418200 tig0018533:-33014 None:intergenic
ATCGAAGCCGGGACCTAGAT+TGG 0.418554 tig0018533:-33027 None:intergenic
TTGCTGTCAATGAGATCTTC+GGG 0.419028 tig0018533:+33330 Msa1367630:CDS
CTTTCACTTTCTCTCGAAAA+GGG 0.421777 tig0018533:+33251 Msa1367630:CDS
GTTGCTGTCAATGAGATCTT+CGG 0.437235 tig0018533:+33329 Msa1367630:CDS
ACGCCACAAGAGCAGCTGCC+TGG 0.444695 tig0018533:-33625 None:intergenic
TCCTACTGCGGTTGCTGTCA+CGG 0.459444 tig0018533:+33802 Msa1367630:CDS
GGAATCAATGGTGCACGCTT+AGG 0.462471 tig0018533:-33710 None:intergenic
GCCAAAAGCTCCAGCTTGAA+TGG 0.464692 tig0018533:-33759 None:intergenic
GGTCCCGGCTTCGATTTCCT+TGG 0.466367 tig0018533:+33035 Msa1367630:CDS
GGCAGGAGCAAAGGCCTTGA+CGG 0.467231 tig0018533:-32997 None:intergenic
GCAGCCAAGGAAATCGAAGC+CGG 0.468475 tig0018533:-33039 None:intergenic
AAGGGTTTACCTTTGGGAAG+TGG 0.480325 tig0018533:+33269 Msa1367630:CDS
CAATGTTGCCCCATCCATCA+TGG 0.480430 tig0018533:+33427 Msa1367630:CDS
AACATCTGGGTCCACTTTGA+CGG 0.481780 tig0018533:-33090 None:intergenic
CTTGTGGCGTCAGTGTTACA+GGG 0.482574 tig0018533:+33638 Msa1367630:CDS
GACAGCAACCGCGGCTGCAG+CGG 0.482714 tig0018533:-33315 None:intergenic
CATGTTGTTGAAGCAGCCAT+TGG 0.487615 tig0018533:-32822 None:intergenic
CTTCTCTTCTGATTTCAAGC+AGG 0.492990 tig0018533:-33384 None:intergenic
CAACAATCTTATCGGAGGAC+AGG 0.494191 tig0018533:-33685 None:intergenic
AAGAAGGCTGCCATTCAAGC+TGG 0.496033 tig0018533:+33749 Msa1367630:CDS
TGTTTGAAATTAAAGGAAAG+TGG 0.496328 tig0018533:-32864 None:intergenic
CCATGTCTGGAATTCTAGCC+AGG 0.497827 tig0018533:+33607 Msa1367630:CDS
AGCGTGCACCATTGATTCCT+GGG 0.501591 tig0018533:+33714 Msa1367630:CDS
TGTCACGGATAACGAGGAAA+AGG 0.505606 tig0018533:+33817 Msa1367630:CDS
GCATCCATCCCAGGAATCAA+TGG 0.507028 tig0018533:-33722 None:intergenic
TGCCATCGACAGCGCAGCCA+AGG 0.508599 tig0018533:-33052 None:intergenic
GTCACGGATAACGAGGAAAA+GGG 0.510190 tig0018533:+33818 Msa1367630:CDS
ACCTGTTTATTCTTCCGTCA+AGG 0.511408 tig0018533:+32983 Msa1367630:CDS
GTCAAGCAGCTTGATCGCCT+TGG 0.519083 tig0018533:+33908 Msa1367630:CDS
GGCTGCGCTGTCGATGGCAT+CGG 0.519529 tig0018533:+33056 Msa1367630:CDS
ACGGAAGAATAAACAGGTGG+TGG 0.522057 tig0018533:-32978 None:intergenic
ACCGTCGCCAATCTAGGTCC+CGG 0.523818 tig0018533:+33020 Msa1367630:CDS
ACGGCATCAATTCCAAAGGT+TGG 0.527087 tig0018533:-33475 None:intergenic
AATGTTGCCCCATCCATCAT+GGG 0.527769 tig0018533:+33428 Msa1367630:CDS
GGCGACGGTGGCAGGAGCAA+AGG 0.532400 tig0018533:-33006 None:intergenic
TAGCCAGGCAGCTGCTCTTG+TGG 0.536109 tig0018533:+33622 Msa1367630:CDS
AACCCTCTTTGGAACTGCGC+TGG 0.536246 tig0018533:+33179 Msa1367630:CDS
CAGCACTGCCTTTAGAGATA+GGG 0.537569 tig0018533:+33576 Msa1367630:CDS
GCGATCAAGCTGCTTGACAT+TGG 0.539004 tig0018533:-33903 None:intergenic
CAGCCAAGGAAATCGAAGCC+GGG 0.542736 tig0018533:-33038 None:intergenic
TTCTTGACAGCATCCATCCC+AGG 0.549795 tig0018533:-33731 None:intergenic
TGCGGCATCCCTATCTCTAA+AGG 0.558659 tig0018533:-33584 None:intergenic
TGGGATGGATGCTGTCAAGA+AGG 0.561159 tig0018533:+33733 Msa1367630:CDS
GCCTTGACGGAAGAATAAAC+AGG 0.565015 tig0018533:-32984 None:intergenic
TCTCACCGGGGTGAACATCT+GGG 0.567871 tig0018533:-33103 None:intergenic
TGCACCATTGATTCCTGGGA+TGG 0.572503 tig0018533:+33718 Msa1367630:CDS
GGATTTGTTCATGTTAGGGA+AGG 0.572617 tig0018533:+33661 Msa1367630:CDS
CTGCTTGAAATCAGAAGAGA+AGG 0.573937 tig0018533:+33385 Msa1367630:CDS
ATGTGAGATGGATATCTCAC+CGG 0.575914 tig0018533:-33117 None:intergenic
AGTAGGACCAGCACCACTGA+TGG 0.581134 tig0018533:-33786 None:intergenic
TGTGAGATGGATATCTCACC+GGG 0.582722 tig0018533:-33116 None:intergenic
TACCAGCGCAGTTCCAAAGA+GGG 0.584554 tig0018533:-33181 None:intergenic
ATTTCAAGCAGGCGAGAACA+AGG 0.584734 tig0018533:-33373 None:intergenic
TCGGTAAGTGTGACGACGGA+GGG 0.586137 tig0018533:-32954 None:intergenic
AGAATAAACAGGTGGTGGGT+CGG 0.588466 tig0018533:-32973 None:intergenic
AACAATCTTATCGGAGGACA+GGG 0.598923 tig0018533:-33684 None:intergenic
TTCAACGGCATCAATTCCAA+AGG 0.604897 tig0018533:-33479 None:intergenic
ATGCTCCGCATTCGATCCGT+CGG 0.611634 tig0018533:+33227 Msa1367630:CDS
GCCGGGACCTAGATTGGCGA+CGG 0.611775 tig0018533:-33021 None:intergenic
TTGACCAGAAATATGTGAGA+TGG 0.612753 tig0018533:-33129 None:intergenic
ATGTTGCCCCATCCATCATG+GGG 0.617492 tig0018533:+33429 Msa1367630:CDS
GTTGCCCCATCCATCATGGG+GGG 0.619596 tig0018533:+33431 Msa1367630:CDS
CTTCGGGAAAAGATTGAGTG+TGG 0.620572 tig0018533:+33346 Msa1367630:CDS
CCTGGCTAGAATTCCAGACA+TGG 0.621126 tig0018533:-33607 None:intergenic
AAAGACCGACGGATCGAATG+CGG 0.626540 tig0018533:-33232 None:intergenic
TGACACTGTCTCCGTCAAAG+TGG 0.628597 tig0018533:+33079 Msa1367630:CDS
GCACATCATTGCTCAACACA+TGG 0.632675 tig0018533:+33841 Msa1367630:CDS
CGGAAGAATAAACAGGTGGT+GGG 0.638411 tig0018533:-32977 None:intergenic
AGGTTCAACAATCTTATCGG+AGG 0.639350 tig0018533:-33690 None:intergenic
CTAGAATTCCAGACATGGTG+CGG 0.639933 tig0018533:-33602 None:intergenic
GATCTCATTGACAGCAACCG+CGG 0.645517 tig0018533:-33324 None:intergenic
TCACGGATAACGAGGAAAAG+GGG 0.649323 tig0018533:+33819 Msa1367630:CDS
TCCGTGACAGCAACCGCAGT+AGG 0.650384 tig0018533:-33803 None:intergenic
TTGTGGCGTCAGTGTTACAG+GGG 0.658642 tig0018533:+33639 Msa1367630:CDS
GGTTGCTGTCACGGATAACG+AGG 0.663758 tig0018533:+33811 Msa1367630:CDS
GATCCCAAACCACTTCCCAA+AGG 0.668028 tig0018533:-33278 None:intergenic
GACGGAGGGATGCTTCTGAG+AGG 0.670246 tig0018533:-32940 None:intergenic
GTCGGTAAGTGTGACGACGG+AGG 0.671687 tig0018533:-32955 None:intergenic
ATACCAGCGCAGTTCCAAAG+AGG 0.675755 tig0018533:-33182 None:intergenic
TGTGGCGTCAGTGTTACAGG+GGG 0.680350 tig0018533:+33640 Msa1367630:CDS
GAGAGAAAGTGAAAGACCGA+CGG 0.682882 tig0018533:-33243 None:intergenic
TGCTAATAAGTCTAGCACCA+AGG 0.687849 tig0018533:-33925 None:intergenic
CAGTGGTGCTGGTCCTACTG+CGG 0.694225 tig0018533:+33790 Msa1367630:CDS
TGTTGCCCCATCCATCATGG+GGG 0.701496 tig0018533:+33430 Msa1367630:CDS
TGGGTCGGTAAGTGTGACGA+CGG 0.704433 tig0018533:-32958 None:intergenic
TTGACGGAAGAATAAACAGG+TGG 0.720068 tig0018533:-32981 None:intergenic
GTGAGATGGATATCTCACCG+GGG 0.829626 tig0018533:-33115 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCTTTTGTTTGAAATTAA+AGG - tig0018533:32874-32893 None:intergenic 15.0%
!!! ATTCTTTTGTTTGAAATTAA+AGG - tig0018533:32874-32893 None:intergenic 15.0%
!! TGTTTGAAATTAAAGGAAAG+TGG - tig0018533:32867-32886 None:intergenic 25.0%
!!! AATATAGCTGTTTTTCAGAT+GGG - tig0018533:33508-33527 None:intergenic 25.0%
!!! AAATATAGCTGTTTTTCAGA+TGG - tig0018533:33509-33528 None:intergenic 25.0%
!! TGTTTGAAATTAAAGGAAAG+TGG - tig0018533:32867-32886 None:intergenic 25.0%
!!! AATATAGCTGTTTTTCAGAT+GGG - tig0018533:33508-33527 None:intergenic 25.0%
!!! AAATATAGCTGTTTTTCAGA+TGG - tig0018533:33509-33528 None:intergenic 25.0%
ATATCCATCTCACATATTTC+TGG + tig0018533:33125-33144 Msa1367630:CDS 30.0%
!!! TGCTCTCATTTTTTTAGTTG+GGG - tig0018533:33558-33577 None:intergenic 30.0%
!!! CTGCTCTCATTTTTTTAGTT+GGG - tig0018533:33559-33578 None:intergenic 30.0%
CTTAGGTTCAACAATCTTAT+CGG - tig0018533:33696-33715 None:intergenic 30.0%
ATATCCATCTCACATATTTC+TGG + tig0018533:33125-33144 Msa1367630:CDS 30.0%
!!! TGCTCTCATTTTTTTAGTTG+GGG - tig0018533:33558-33577 None:intergenic 30.0%
!!! CTGCTCTCATTTTTTTAGTT+GGG - tig0018533:33559-33578 None:intergenic 30.0%
CTTAGGTTCAACAATCTTAT+CGG - tig0018533:33696-33715 None:intergenic 30.0%
TTGACCAGAAATATGTGAGA+TGG - tig0018533:33132-33151 None:intergenic 35.0%
TTACTGAGTTTGTGGATGTT+AGG - tig0018533:33158-33177 None:intergenic 35.0%
AACTCAGTAAAAACCCTCTT+TGG + tig0018533:33168-33187 Msa1367630:CDS 35.0%
TCTTTCACTTTCTCTCGAAA+AGG + tig0018533:33250-33269 Msa1367630:CDS 35.0%
CTTTCACTTTCTCTCGAAAA+GGG + tig0018533:33251-33270 Msa1367630:CDS 35.0%
AAATCAGAAGAGAAGGTTTC+TGG + tig0018533:33392-33411 Msa1367630:CDS 35.0%
! TTTTCAGATGGGAACTTCAA+CGG - tig0018533:33497-33516 None:intergenic 35.0%
!!! GCTGCTCTCATTTTTTTAGT+TGG - tig0018533:33560-33579 None:intergenic 35.0%
! CATGGTTCAAGCTTTTCAAA+AGG + tig0018533:33859-33878 Msa1367630:CDS 35.0%
! GTTCAAGCTTTTCAAAAGGA+AGG + tig0018533:33863-33882 Msa1367630:CDS 35.0%
TTGACCAGAAATATGTGAGA+TGG - tig0018533:33132-33151 None:intergenic 35.0%
TTACTGAGTTTGTGGATGTT+AGG - tig0018533:33158-33177 None:intergenic 35.0%
AACTCAGTAAAAACCCTCTT+TGG + tig0018533:33168-33187 Msa1367630:CDS 35.0%
TCTTTCACTTTCTCTCGAAA+AGG + tig0018533:33250-33269 Msa1367630:CDS 35.0%
CTTTCACTTTCTCTCGAAAA+GGG + tig0018533:33251-33270 Msa1367630:CDS 35.0%
AAATCAGAAGAGAAGGTTTC+TGG + tig0018533:33392-33411 Msa1367630:CDS 35.0%
! TTTTCAGATGGGAACTTCAA+CGG - tig0018533:33497-33516 None:intergenic 35.0%
!!! GCTGCTCTCATTTTTTTAGT+TGG - tig0018533:33560-33579 None:intergenic 35.0%
! CATGGTTCAAGCTTTTCAAA+AGG + tig0018533:33859-33878 Msa1367630:CDS 35.0%
! GTTCAAGCTTTTCAAAAGGA+AGG + tig0018533:33863-33882 Msa1367630:CDS 35.0%
TTGACGGAAGAATAAACAGG+TGG - tig0018533:32984-33003 None:intergenic 40.0%
ACCTGTTTATTCTTCCGTCA+AGG + tig0018533:32983-33002 Msa1367630:CDS 40.0%
ATGTGAGATGGATATCTCAC+CGG - tig0018533:33120-33139 None:intergenic 40.0%
! GAGGGTTTTTACTGAGTTTG+TGG - tig0018533:33166-33185 None:intergenic 40.0%
! CATTTTCATGGCTTCGATAG+CGG - tig0018533:33210-33229 None:intergenic 40.0%
TCTCGAAAAGGGTTTACCTT+TGG + tig0018533:33262-33281 Msa1367630:CDS 40.0%
CTCGAAAAGGGTTTACCTTT+GGG + tig0018533:33263-33282 Msa1367630:CDS 40.0%
! TTTACCTTTGGGAAGTGGTT+TGG + tig0018533:33274-33293 Msa1367630:CDS 40.0%
! TTACCTTTGGGAAGTGGTTT+GGG + tig0018533:33275-33294 Msa1367630:CDS 40.0%
GTTGCTGTCAATGAGATCTT+CGG + tig0018533:33329-33348 Msa1367630:CDS 40.0%
TTGCTGTCAATGAGATCTTC+GGG + tig0018533:33330-33349 Msa1367630:CDS 40.0%
CTTCTCTTCTGATTTCAAGC+AGG - tig0018533:33387-33406 None:intergenic 40.0%
CTGCTTGAAATCAGAAGAGA+AGG + tig0018533:33385-33404 Msa1367630:CDS 40.0%
GATTCGCAATTACCAACCTT+TGG + tig0018533:33463-33482 Msa1367630:CDS 40.0%
TTCAACGGCATCAATTCCAA+AGG - tig0018533:33482-33501 None:intergenic 40.0%
TTAGTTGGGGCTTCAAATTC+TGG - tig0018533:33545-33564 None:intergenic 40.0%
! AGGGGGATTTGTTCATGTTA+GGG + tig0018533:33657-33676 Msa1367630:CDS 40.0%
! GGATTTGTTCATGTTAGGGA+AGG + tig0018533:33661-33680 Msa1367630:CDS 40.0%
AACAATCTTATCGGAGGACA+GGG - tig0018533:33687-33706 None:intergenic 40.0%
AGGTTCAACAATCTTATCGG+AGG - tig0018533:33693-33712 None:intergenic 40.0%
TGCTAATAAGTCTAGCACCA+AGG - tig0018533:33928-33947 None:intergenic 40.0%
TTGACGGAAGAATAAACAGG+TGG - tig0018533:32984-33003 None:intergenic 40.0%
ACCTGTTTATTCTTCCGTCA+AGG + tig0018533:32983-33002 Msa1367630:CDS 40.0%
ATGTGAGATGGATATCTCAC+CGG - tig0018533:33120-33139 None:intergenic 40.0%
! GAGGGTTTTTACTGAGTTTG+TGG - tig0018533:33166-33185 None:intergenic 40.0%
! CATTTTCATGGCTTCGATAG+CGG - tig0018533:33210-33229 None:intergenic 40.0%
TCTCGAAAAGGGTTTACCTT+TGG + tig0018533:33262-33281 Msa1367630:CDS 40.0%
CTCGAAAAGGGTTTACCTTT+GGG + tig0018533:33263-33282 Msa1367630:CDS 40.0%
! TTTACCTTTGGGAAGTGGTT+TGG + tig0018533:33274-33293 Msa1367630:CDS 40.0%
! TTACCTTTGGGAAGTGGTTT+GGG + tig0018533:33275-33294 Msa1367630:CDS 40.0%
GTTGCTGTCAATGAGATCTT+CGG + tig0018533:33329-33348 Msa1367630:CDS 40.0%
TTGCTGTCAATGAGATCTTC+GGG + tig0018533:33330-33349 Msa1367630:CDS 40.0%
CTTCTCTTCTGATTTCAAGC+AGG - tig0018533:33387-33406 None:intergenic 40.0%
CTGCTTGAAATCAGAAGAGA+AGG + tig0018533:33385-33404 Msa1367630:CDS 40.0%
GATTCGCAATTACCAACCTT+TGG + tig0018533:33463-33482 Msa1367630:CDS 40.0%
TTCAACGGCATCAATTCCAA+AGG - tig0018533:33482-33501 None:intergenic 40.0%
TTAGTTGGGGCTTCAAATTC+TGG - tig0018533:33545-33564 None:intergenic 40.0%
! AGGGGGATTTGTTCATGTTA+GGG + tig0018533:33657-33676 Msa1367630:CDS 40.0%
! GGATTTGTTCATGTTAGGGA+AGG + tig0018533:33661-33680 Msa1367630:CDS 40.0%
AACAATCTTATCGGAGGACA+GGG - tig0018533:33687-33706 None:intergenic 40.0%
AGGTTCAACAATCTTATCGG+AGG - tig0018533:33693-33712 None:intergenic 40.0%
TGCTAATAAGTCTAGCACCA+AGG - tig0018533:33928-33947 None:intergenic 40.0%
!! AGAATAAACAGGTGGTGGGT+CGG - tig0018533:32976-32995 None:intergenic 45.0%
CGGAAGAATAAACAGGTGGT+GGG - tig0018533:32980-32999 None:intergenic 45.0%
ACGGAAGAATAAACAGGTGG+TGG - tig0018533:32981-33000 None:intergenic 45.0%
GCCTTGACGGAAGAATAAAC+AGG - tig0018533:32987-33006 None:intergenic 45.0%
AACATCTGGGTCCACTTTGA+CGG - tig0018533:33093-33112 None:intergenic 45.0%
TGTGAGATGGATATCTCACC+GGG - tig0018533:33119-33138 None:intergenic 45.0%
!! TCGAATGCGGAGCATTTTCA+TGG - tig0018533:33222-33241 None:intergenic 45.0%
GAGAGAAAGTGAAAGACCGA+CGG - tig0018533:33246-33265 None:intergenic 45.0%
! AAGGGTTTACCTTTGGGAAG+TGG + tig0018533:33269-33288 Msa1367630:CDS 45.0%
CTTCGGGAAAAGATTGAGTG+TGG + tig0018533:33346-33365 Msa1367630:CDS 45.0%
ATTTCAAGCAGGCGAGAACA+AGG - tig0018533:33376-33395 None:intergenic 45.0%
AATGTTGCCCCATCCATCAT+GGG + tig0018533:33428-33447 Msa1367630:CDS 45.0%
ACGGCATCAATTCCAAAGGT+TGG - tig0018533:33478-33497 None:intergenic 45.0%
! CAGCACTGCCTTTAGAGATA+GGG + tig0018533:33576-33595 Msa1367630:CDS 45.0%
CTAGAATTCCAGACATGGTG+CGG - tig0018533:33605-33624 None:intergenic 45.0%
! CAGGGGGATTTGTTCATGTT+AGG + tig0018533:33656-33675 Msa1367630:CDS 45.0%
CAACAATCTTATCGGAGGAC+AGG - tig0018533:33688-33707 None:intergenic 45.0%
TGTCACGGATAACGAGGAAA+AGG + tig0018533:33817-33836 Msa1367630:CDS 45.0%
GTCACGGATAACGAGGAAAA+GGG + tig0018533:33818-33837 Msa1367630:CDS 45.0%
TCACGGATAACGAGGAAAAG+GGG + tig0018533:33819-33838 Msa1367630:CDS 45.0%
GCACATCATTGCTCAACACA+TGG + tig0018533:33841-33860 Msa1367630:CDS 45.0%
!! AGAATAAACAGGTGGTGGGT+CGG - tig0018533:32976-32995 None:intergenic 45.0%
CGGAAGAATAAACAGGTGGT+GGG - tig0018533:32980-32999 None:intergenic 45.0%
ACGGAAGAATAAACAGGTGG+TGG - tig0018533:32981-33000 None:intergenic 45.0%
GCCTTGACGGAAGAATAAAC+AGG - tig0018533:32987-33006 None:intergenic 45.0%
AACATCTGGGTCCACTTTGA+CGG - tig0018533:33093-33112 None:intergenic 45.0%
TGTGAGATGGATATCTCACC+GGG - tig0018533:33119-33138 None:intergenic 45.0%
!! TCGAATGCGGAGCATTTTCA+TGG - tig0018533:33222-33241 None:intergenic 45.0%
GAGAGAAAGTGAAAGACCGA+CGG - tig0018533:33246-33265 None:intergenic 45.0%
! AAGGGTTTACCTTTGGGAAG+TGG + tig0018533:33269-33288 Msa1367630:CDS 45.0%
CTTCGGGAAAAGATTGAGTG+TGG + tig0018533:33346-33365 Msa1367630:CDS 45.0%
ATTTCAAGCAGGCGAGAACA+AGG - tig0018533:33376-33395 None:intergenic 45.0%
AATGTTGCCCCATCCATCAT+GGG + tig0018533:33428-33447 Msa1367630:CDS 45.0%
ACGGCATCAATTCCAAAGGT+TGG - tig0018533:33478-33497 None:intergenic 45.0%
! CAGCACTGCCTTTAGAGATA+GGG + tig0018533:33576-33595 Msa1367630:CDS 45.0%
CTAGAATTCCAGACATGGTG+CGG - tig0018533:33605-33624 None:intergenic 45.0%
! CAGGGGGATTTGTTCATGTT+AGG + tig0018533:33656-33675 Msa1367630:CDS 45.0%
CAACAATCTTATCGGAGGAC+AGG - tig0018533:33688-33707 None:intergenic 45.0%
TGTCACGGATAACGAGGAAA+AGG + tig0018533:33817-33836 Msa1367630:CDS 45.0%
GTCACGGATAACGAGGAAAA+GGG + tig0018533:33818-33837 Msa1367630:CDS 45.0%
TCACGGATAACGAGGAAAAG+GGG + tig0018533:33819-33838 Msa1367630:CDS 45.0%
GCACATCATTGCTCAACACA+TGG + tig0018533:33841-33860 Msa1367630:CDS 45.0%
TGACACTGTCTCCGTCAAAG+TGG + tig0018533:33079-33098 Msa1367630:CDS 50.0%
GTGAGATGGATATCTCACCG+GGG - tig0018533:33118-33137 None:intergenic 50.0%
TACCAGCGCAGTTCCAAAGA+GGG - tig0018533:33184-33203 None:intergenic 50.0%
ATACCAGCGCAGTTCCAAAG+AGG - tig0018533:33185-33204 None:intergenic 50.0%
AAAGACCGACGGATCGAATG+CGG - tig0018533:33235-33254 None:intergenic 50.0%
GATCCCAAACCACTTCCCAA+AGG - tig0018533:33281-33300 None:intergenic 50.0%
GATCTCATTGACAGCAACCG+CGG - tig0018533:33327-33346 None:intergenic 50.0%
CAATGTTGCCCCATCCATCA+TGG + tig0018533:33427-33446 Msa1367630:CDS 50.0%
ATGTTGCCCCATCCATCATG+GGG + tig0018533:33429-33448 Msa1367630:CDS 50.0%
!! AAAACCCCCCATGATGGATG+GGG - tig0018533:33438-33457 None:intergenic 50.0%
!! CAAAACCCCCCATGATGGAT+GGG - tig0018533:33439-33458 None:intergenic 50.0%
! ACAAAACCCCCCATGATGGA+TGG - tig0018533:33440-33459 None:intergenic 50.0%
CAACACAAAACCCCCCATGA+TGG - tig0018533:33444-33463 None:intergenic 50.0%
! GCAGCACTGCCTTTAGAGAT+AGG + tig0018533:33575-33594 Msa1367630:CDS 50.0%
TGCGGCATCCCTATCTCTAA+AGG - tig0018533:33587-33606 None:intergenic 50.0%
CCTGGCTAGAATTCCAGACA+TGG - tig0018533:33610-33629 None:intergenic 50.0%
! CCATGTCTGGAATTCTAGCC+AGG + tig0018533:33607-33626 Msa1367630:CDS 50.0%
TCTTGTGGCGTCAGTGTTAC+AGG + tig0018533:33637-33656 Msa1367630:CDS 50.0%
CTTGTGGCGTCAGTGTTACA+GGG + tig0018533:33638-33657 Msa1367630:CDS 50.0%
TTGTGGCGTCAGTGTTACAG+GGG + tig0018533:33639-33658 Msa1367630:CDS 50.0%
GGAATCAATGGTGCACGCTT+AGG - tig0018533:33713-33732 None:intergenic 50.0%
!! AAGCGTGCACCATTGATTCC+TGG + tig0018533:33713-33732 Msa1367630:CDS 50.0%
!! AGCGTGCACCATTGATTCCT+GGG + tig0018533:33714-33733 Msa1367630:CDS 50.0%
!! TGCACCATTGATTCCTGGGA+TGG + tig0018533:33718-33737 Msa1367630:CDS 50.0%
GCATCCATCCCAGGAATCAA+TGG - tig0018533:33725-33744 None:intergenic 50.0%
TTCTTGACAGCATCCATCCC+AGG - tig0018533:33734-33753 None:intergenic 50.0%
TGGGATGGATGCTGTCAAGA+AGG + tig0018533:33733-33752 Msa1367630:CDS 50.0%
AAGAAGGCTGCCATTCAAGC+TGG + tig0018533:33749-33768 Msa1367630:CDS 50.0%
! GCCATTCAAGCTGGAGCTTT+TGG + tig0018533:33758-33777 Msa1367630:CDS 50.0%
GCCAAAAGCTCCAGCTTGAA+TGG - tig0018533:33762-33781 None:intergenic 50.0%
GCGATCAAGCTGCTTGACAT+TGG - tig0018533:33906-33925 None:intergenic 50.0%
TGACACTGTCTCCGTCAAAG+TGG + tig0018533:33079-33098 Msa1367630:CDS 50.0%
GTGAGATGGATATCTCACCG+GGG - tig0018533:33118-33137 None:intergenic 50.0%
TACCAGCGCAGTTCCAAAGA+GGG - tig0018533:33184-33203 None:intergenic 50.0%
ATACCAGCGCAGTTCCAAAG+AGG - tig0018533:33185-33204 None:intergenic 50.0%
AAAGACCGACGGATCGAATG+CGG - tig0018533:33235-33254 None:intergenic 50.0%
GATCCCAAACCACTTCCCAA+AGG - tig0018533:33281-33300 None:intergenic 50.0%
GATCTCATTGACAGCAACCG+CGG - tig0018533:33327-33346 None:intergenic 50.0%
CAATGTTGCCCCATCCATCA+TGG + tig0018533:33427-33446 Msa1367630:CDS 50.0%
ATGTTGCCCCATCCATCATG+GGG + tig0018533:33429-33448 Msa1367630:CDS 50.0%
!! AAAACCCCCCATGATGGATG+GGG - tig0018533:33438-33457 None:intergenic 50.0%
!! CAAAACCCCCCATGATGGAT+GGG - tig0018533:33439-33458 None:intergenic 50.0%
! ACAAAACCCCCCATGATGGA+TGG - tig0018533:33440-33459 None:intergenic 50.0%
CAACACAAAACCCCCCATGA+TGG - tig0018533:33444-33463 None:intergenic 50.0%
! GCAGCACTGCCTTTAGAGAT+AGG + tig0018533:33575-33594 Msa1367630:CDS 50.0%
TGCGGCATCCCTATCTCTAA+AGG - tig0018533:33587-33606 None:intergenic 50.0%
CCTGGCTAGAATTCCAGACA+TGG - tig0018533:33610-33629 None:intergenic 50.0%
! CCATGTCTGGAATTCTAGCC+AGG + tig0018533:33607-33626 Msa1367630:CDS 50.0%
TCTTGTGGCGTCAGTGTTAC+AGG + tig0018533:33637-33656 Msa1367630:CDS 50.0%
CTTGTGGCGTCAGTGTTACA+GGG + tig0018533:33638-33657 Msa1367630:CDS 50.0%
TTGTGGCGTCAGTGTTACAG+GGG + tig0018533:33639-33658 Msa1367630:CDS 50.0%
GGAATCAATGGTGCACGCTT+AGG - tig0018533:33713-33732 None:intergenic 50.0%
!! AAGCGTGCACCATTGATTCC+TGG + tig0018533:33713-33732 Msa1367630:CDS 50.0%
!! AGCGTGCACCATTGATTCCT+GGG + tig0018533:33714-33733 Msa1367630:CDS 50.0%
!! TGCACCATTGATTCCTGGGA+TGG + tig0018533:33718-33737 Msa1367630:CDS 50.0%
GCATCCATCCCAGGAATCAA+TGG - tig0018533:33725-33744 None:intergenic 50.0%
TTCTTGACAGCATCCATCCC+AGG - tig0018533:33734-33753 None:intergenic 50.0%
TGGGATGGATGCTGTCAAGA+AGG + tig0018533:33733-33752 Msa1367630:CDS 50.0%
AAGAAGGCTGCCATTCAAGC+TGG + tig0018533:33749-33768 Msa1367630:CDS 50.0%
! GCCATTCAAGCTGGAGCTTT+TGG + tig0018533:33758-33777 Msa1367630:CDS 50.0%
GCCAAAAGCTCCAGCTTGAA+TGG - tig0018533:33762-33781 None:intergenic 50.0%
GCGATCAAGCTGCTTGACAT+TGG - tig0018533:33906-33925 None:intergenic 50.0%
TCGGTAAGTGTGACGACGGA+GGG - tig0018533:32957-32976 None:intergenic 55.0%
TGGGTCGGTAAGTGTGACGA+CGG - tig0018533:32961-32980 None:intergenic 55.0%
ATCGAAGCCGGGACCTAGAT+TGG - tig0018533:33030-33049 None:intergenic 55.0%
CAGCCAAGGAAATCGAAGCC+GGG - tig0018533:33041-33060 None:intergenic 55.0%
GCAGCCAAGGAAATCGAAGC+CGG - tig0018533:33042-33061 None:intergenic 55.0%
TCTCACCGGGGTGAACATCT+GGG - tig0018533:33106-33125 None:intergenic 55.0%
ATCTCACCGGGGTGAACATC+TGG - tig0018533:33107-33126 None:intergenic 55.0%
!! AACCCTCTTTGGAACTGCGC+TGG + tig0018533:33179-33198 Msa1367630:CDS 55.0%
ATGCTCCGCATTCGATCCGT+CGG + tig0018533:33227-33246 Msa1367630:CDS 55.0%
TGTTGCCCCATCCATCATGG+GGG + tig0018533:33430-33449 Msa1367630:CDS 55.0%
TGTGGCGTCAGTGTTACAGG+GGG + tig0018533:33640-33659 Msa1367630:CDS 55.0%
!! GCTTTTGGCTGCACCATCAG+TGG + tig0018533:33773-33792 Msa1367630:CDS 55.0%
AGTAGGACCAGCACCACTGA+TGG - tig0018533:33789-33808 None:intergenic 55.0%
TCCTACTGCGGTTGCTGTCA+CGG + tig0018533:33802-33821 Msa1367630:CDS 55.0%
GGTTGCTGTCACGGATAACG+AGG + tig0018533:33811-33830 Msa1367630:CDS 55.0%
GTCAAGCAGCTTGATCGCCT+TGG + tig0018533:33908-33927 Msa1367630:CDS 55.0%
TCGGTAAGTGTGACGACGGA+GGG - tig0018533:32957-32976 None:intergenic 55.0%
TGGGTCGGTAAGTGTGACGA+CGG - tig0018533:32961-32980 None:intergenic 55.0%
ATCGAAGCCGGGACCTAGAT+TGG - tig0018533:33030-33049 None:intergenic 55.0%
CAGCCAAGGAAATCGAAGCC+GGG - tig0018533:33041-33060 None:intergenic 55.0%
GCAGCCAAGGAAATCGAAGC+CGG - tig0018533:33042-33061 None:intergenic 55.0%
TCTCACCGGGGTGAACATCT+GGG - tig0018533:33106-33125 None:intergenic 55.0%
ATCTCACCGGGGTGAACATC+TGG - tig0018533:33107-33126 None:intergenic 55.0%
!! AACCCTCTTTGGAACTGCGC+TGG + tig0018533:33179-33198 Msa1367630:CDS 55.0%
ATGCTCCGCATTCGATCCGT+CGG + tig0018533:33227-33246 Msa1367630:CDS 55.0%
TGTTGCCCCATCCATCATGG+GGG + tig0018533:33430-33449 Msa1367630:CDS 55.0%
TGTGGCGTCAGTGTTACAGG+GGG + tig0018533:33640-33659 Msa1367630:CDS 55.0%
!! GCTTTTGGCTGCACCATCAG+TGG + tig0018533:33773-33792 Msa1367630:CDS 55.0%
AGTAGGACCAGCACCACTGA+TGG - tig0018533:33789-33808 None:intergenic 55.0%
TCCTACTGCGGTTGCTGTCA+CGG + tig0018533:33802-33821 Msa1367630:CDS 55.0%
GGTTGCTGTCACGGATAACG+AGG + tig0018533:33811-33830 Msa1367630:CDS 55.0%
GTCAAGCAGCTTGATCGCCT+TGG + tig0018533:33908-33927 Msa1367630:CDS 55.0%
GACGGAGGGATGCTTCTGAG+AGG - tig0018533:32943-32962 None:intergenic 60.0%
GTCGGTAAGTGTGACGACGG+AGG - tig0018533:32958-32977 None:intergenic 60.0%
GGCAGGAGCAAAGGCCTTGA+CGG - tig0018533:33000-33019 None:intergenic 60.0%
ACCGTCGCCAATCTAGGTCC+CGG + tig0018533:33020-33039 Msa1367630:CDS 60.0%
GGTCCCGGCTTCGATTTCCT+TGG + tig0018533:33035-33054 Msa1367630:CDS 60.0%
TTCCTTGGCTGCGCTGTCGA+TGG + tig0018533:33050-33069 Msa1367630:CDS 60.0%
GTGGACCCAGATGTTCACCC+CGG + tig0018533:33098-33117 Msa1367630:CDS 60.0%
GTTGCCCCATCCATCATGGG+GGG + tig0018533:33431-33450 Msa1367630:CDS 60.0%
TAGGGATGCCGCACCATGTC+TGG + tig0018533:33594-33613 Msa1367630:CDS 60.0%
TAGCCAGGCAGCTGCTCTTG+TGG + tig0018533:33622-33641 Msa1367630:CDS 60.0%
!! CAGTGGTGCTGGTCCTACTG+CGG + tig0018533:33790-33809 Msa1367630:CDS 60.0%
TCCGTGACAGCAACCGCAGT+AGG - tig0018533:33806-33825 None:intergenic 60.0%
GACGGAGGGATGCTTCTGAG+AGG - tig0018533:32943-32962 None:intergenic 60.0%
GTCGGTAAGTGTGACGACGG+AGG - tig0018533:32958-32977 None:intergenic 60.0%
GGCAGGAGCAAAGGCCTTGA+CGG - tig0018533:33000-33019 None:intergenic 60.0%
ACCGTCGCCAATCTAGGTCC+CGG + tig0018533:33020-33039 Msa1367630:CDS 60.0%
GGTCCCGGCTTCGATTTCCT+TGG + tig0018533:33035-33054 Msa1367630:CDS 60.0%
TTCCTTGGCTGCGCTGTCGA+TGG + tig0018533:33050-33069 Msa1367630:CDS 60.0%
GTGGACCCAGATGTTCACCC+CGG + tig0018533:33098-33117 Msa1367630:CDS 60.0%
GTTGCCCCATCCATCATGGG+GGG + tig0018533:33431-33450 Msa1367630:CDS 60.0%
TAGGGATGCCGCACCATGTC+TGG + tig0018533:33594-33613 Msa1367630:CDS 60.0%
TAGCCAGGCAGCTGCTCTTG+TGG + tig0018533:33622-33641 Msa1367630:CDS 60.0%
!! CAGTGGTGCTGGTCCTACTG+CGG + tig0018533:33790-33809 Msa1367630:CDS 60.0%
TCCGTGACAGCAACCGCAGT+AGG - tig0018533:33806-33825 None:intergenic 60.0%
CCTAGATTGGCGACGGTGGC+AGG - tig0018533:33017-33036 None:intergenic 65.0%
CCTGCCACCGTCGCCAATCT+AGG + tig0018533:33014-33033 Msa1367630:CDS 65.0%
GGGACCTAGATTGGCGACGG+TGG - tig0018533:33021-33040 None:intergenic 65.0%
GCCGGGACCTAGATTGGCGA+CGG - tig0018533:33024-33043 None:intergenic 65.0%
TGCCATCGACAGCGCAGCCA+AGG - tig0018533:33055-33074 None:intergenic 65.0%
GGCTGCGCTGTCGATGGCAT+CGG + tig0018533:33056-33075 Msa1367630:CDS 65.0%
ACGCCACAAGAGCAGCTGCC+TGG - tig0018533:33628-33647 None:intergenic 65.0%
GGCTGCACCATCAGTGGTGC+TGG + tig0018533:33779-33798 Msa1367630:CDS 65.0%
CCTAGATTGGCGACGGTGGC+AGG - tig0018533:33017-33036 None:intergenic 65.0%
CCTGCCACCGTCGCCAATCT+AGG + tig0018533:33014-33033 Msa1367630:CDS 65.0%
GGGACCTAGATTGGCGACGG+TGG - tig0018533:33021-33040 None:intergenic 65.0%
GCCGGGACCTAGATTGGCGA+CGG - tig0018533:33024-33043 None:intergenic 65.0%
TGCCATCGACAGCGCAGCCA+AGG - tig0018533:33055-33074 None:intergenic 65.0%
GGCTGCGCTGTCGATGGCAT+CGG + tig0018533:33056-33075 Msa1367630:CDS 65.0%
ACGCCACAAGAGCAGCTGCC+TGG - tig0018533:33628-33647 None:intergenic 65.0%
GGCTGCACCATCAGTGGTGC+TGG + tig0018533:33779-33798 Msa1367630:CDS 65.0%
GGCGACGGTGGCAGGAGCAA+AGG - tig0018533:33009-33028 None:intergenic 70.0%
GACAGCAACCGCGGCTGCAG+CGG - tig0018533:33318-33337 None:intergenic 70.0%
GGCGACGGTGGCAGGAGCAA+AGG - tig0018533:33009-33028 None:intergenic 70.0%
GACAGCAACCGCGGCTGCAG+CGG - tig0018533:33318-33337 None:intergenic 70.0%
!! TGCAGCGGCGCTGGCAGCAC+TGG - tig0018533:33303-33322 None:intergenic 75.0%
!! TGCAGCGGCGCTGGCAGCAC+TGG - tig0018533:33303-33322 None:intergenic 75.0%
! TGCCAGCGCCGCTGCAGCCG+CGG + tig0018533:33307-33326 Msa1367630:CDS 80.0%
! AACCGCGGCTGCAGCGGCGC+TGG - tig0018533:33312-33331 None:intergenic 80.0%
! TGCCAGCGCCGCTGCAGCCG+CGG + tig0018533:33307-33326 Msa1367630:CDS 80.0%
! AACCGCGGCTGCAGCGGCGC+TGG - tig0018533:33312-33331 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
tig0018533 gene 32825 33964 32825 ID=Msa1367630;Name=Msa1367630
tig0018533 mRNA 32825 33964 32825 ID=Msa1367630-mRNA-1;Parent=Msa1367630;Name=Msa1367630-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|-1|0|1|-1|1|1|0|379
tig0018533 exon 32825 33964 32825 ID=Msa1367630-mRNA-1:exon:23;Parent=Msa1367630-mRNA-1
tig0018533 CDS 32825 33964 32825 ID=Msa1367630-mRNA-1:cds;Parent=Msa1367630-mRNA-1
Gene Sequence

>Msa1367630

ATGGCTGCTTCAACAACATGCTTTTTTCAGTCTTCTTCTCCACTTTCCTTTAATTTCAAACAAAAGAATAATCAAAATATTCATATTCATATTTCAATTTTTAGAATCAGATGCTCCTCTCAGAAGCATCCCTCCGTCGTCACACTTACCGACCCACCACCTGTTTATTCTTCCGTCAAGGCCTTTGCTCCTGCCACCGTCGCCAATCTAGGTCCCGGCTTCGATTTCCTTGGCTGCGCTGTCGATGGCATCGGTGACACTGTCTCCGTCAAAGTGGACCCAGATGTTCACCCCGGTGAGATATCCATCTCACATATTTCTGGTCAAACACCTAACATCCACAAACTCAGTAAAAACCCTCTTTGGAACTGCGCTGGTATCGCCGCTATCGAAGCCATGAAAATGCTCCGCATTCGATCCGTCGGTCTTTCACTTTCTCTCGAAAAGGGTTTACCTTTGGGAAGTGGTTTGGGATCCAGTGCTGCCAGCGCCGCTGCAGCCGCGGTTGCTGTCAATGAGATCTTCGGGAAAAGATTGAGTGTGGATGACCTTGTTCTCGCCTGCTTGAAATCAGAAGAGAAGGTTTCTGGTTATCACGCAGACAATGTTGCCCCATCCATCATGGGGGGTTTTGTGTTGATTCGCAATTACCAACCTTTGGAATTGATGCCGTTGAAGTTCCCATCTGAAAAACAGCTATATTTCGTATTAGTTACACCAGAATTTGAAGCCCCAACTAAAAAAATGAGAGCAGCACTGCCTTTAGAGATAGGGATGCCGCACCATGTCTGGAATTCTAGCCAGGCAGCTGCTCTTGTGGCGTCAGTGTTACAGGGGGATTTGTTCATGTTAGGGAAGGCCCTGTCCTCCGATAAGATTGTTGAACCTAAGCGTGCACCATTGATTCCTGGGATGGATGCTGTCAAGAAGGCTGCCATTCAAGCTGGAGCTTTTGGCTGCACCATCAGTGGTGCTGGTCCTACTGCGGTTGCTGTCACGGATAACGAGGAAAAGGGGCACATCATTGCTCAACACATGGTTCAAGCTTTTCAAAAGGAAGGAAACTTGAATGCTTCTGCCAATGTCAAGCAGCTTGATCGCCTTGGTGCTAGACTTATTAGCAGCGTTCATTTAAATTGA
Protein sequence

>Msa1367630

MAASTTCFFQSSSPLSFNFKQKNNQNIHIHISIFRIRCSSQKHPSVVTLTDPPPVYSSVKAFAPATVANLGPGFDFLGCAVDGIGDTVSVKVDPDVHPGEISISHISGQTPNIHKLSKNPLWNCAGIAAIEAMKMLRIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAVAVNEIFGKRLSVDDLVLACLKSEEKVSGYHADNVAPSIMGGFVLIRNYQPLELMPLKFPSEKQLYFVLVTPEFEAPTKKMRAALPLEIGMPHHVWNSSQAAALVASVLQGDLFMLGKALSSDKIVEPKRAPLIPGMDAVKKAAIQAGAFGCTISGAGPTAVAVTDNEEKGHIIAQHMVQAFQKEGNLNASANVKQLDRLGARLISSVHLN*