AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1399240

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1399240 MtrunA17_Chr5g0448691 96.063 381 8 4 3 380 2 378 0.0 728
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1399240 AT2G17265.1 70.889 371 98 4 9 377 6 368 3.42e-164 464
Msa1399240 AT4G35295.1 57.143 63 22 1 315 377 54 111 3.05e-13 66.2

Find 110 sgRNAs with CRISPR-Local

Find 264 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAGTTGGGGCTTCAAATTC+TGG 0.168994 tig0022346:+34262 None:intergenic
AAATCAGAAGAGAAGGTTTC+TGG 0.179307 tig0022346:-34412 Msa1399240:CDS
TCTTGTGGCGTCAGTGTTAC+AGG 0.194081 tig0022346:-34167 Msa1399240:CDS
GCCATTCAAGCTGGAGCTTT+TGG 0.204037 tig0022346:-34046 Msa1399240:CDS
TTACCTTTGGGAAGTGGTTT+GGG 0.235266 tig0022346:-34529 Msa1399240:CDS
TTTACCTTTGGGAAGTGGTT+TGG 0.257806 tig0022346:-34530 Msa1399240:CDS
ATATCCATCTCACATATTTC+TGG 0.261329 tig0022346:-34679 Msa1399240:CDS
ATCTCACCGGGGTGAACATC+TGG 0.273214 tig0022346:+34700 None:intergenic
CCTGCCACCGTCGCCAATCT+AGG 0.297972 tig0022346:-34790 Msa1399240:CDS
TGCAGCGGCGCTGGCAGCAC+TGG 0.313196 tig0022346:+34504 None:intergenic
TCTCGAAAAGGGTTTACCTT+TGG 0.330871 tig0022346:-34542 Msa1399240:CDS
TTACTGAGTTTGTGGATGTT+AGG 0.331672 tig0022346:+34649 None:intergenic
CTCGAAAAGGGTTTACCTTT+GGG 0.332798 tig0022346:-34541 Msa1399240:CDS
CTTAGGTTCAACAATCTTAT+CGG 0.352515 tig0022346:+34111 None:intergenic
GCAGCACTGCCTTTAGAGAT+AGG 0.366224 tig0022346:-34229 Msa1399240:CDS
TCTTTCACTTTCTCTCGAAA+AGG 0.371475 tig0022346:-34554 Msa1399240:CDS
TAGGGATGCCGCACCATGTC+TGG 0.375083 tig0022346:-34210 Msa1399240:CDS
AAGCGTGCACCATTGATTCC+TGG 0.387995 tig0022346:-34091 Msa1399240:CDS
GATTCGCAATTACCAACCTT+TGG 0.392630 tig0022346:-34341 Msa1399240:CDS
TGCCAGCGCCGCTGCAGCCG+CGG 0.400058 tig0022346:-34497 Msa1399240:CDS
AACCGCGGCTGCAGCGGCGC+TGG 0.405837 tig0022346:+34495 None:intergenic
GGCTGCACCATCAGTGGTGC+TGG 0.412382 tig0022346:-34025 Msa1399240:CDS
CCTAGATTGGCGACGGTGGC+AGG 0.418200 tig0022346:+34790 None:intergenic
ATCGAAGCCGGGACCTAGAT+TGG 0.418554 tig0022346:+34777 None:intergenic
TTGCTGTCAATGAGATCTTC+GGG 0.419028 tig0022346:-34474 Msa1399240:CDS
CTTTCACTTTCTCTCGAAAA+GGG 0.421777 tig0022346:-34553 Msa1399240:CDS
GTTGCTGTCAATGAGATCTT+CGG 0.437235 tig0022346:-34475 Msa1399240:CDS
ACGCCACAAGAGCAGCTGCC+TGG 0.444695 tig0022346:+34179 None:intergenic
TCCTACTGCGGTTGCTGTCA+CGG 0.459444 tig0022346:-34002 Msa1399240:CDS
GGAATCAATGGTGCACGCTT+AGG 0.462471 tig0022346:+34094 None:intergenic
GCCAAAAGCTCCAGCTTGAA+TGG 0.464692 tig0022346:+34045 None:intergenic
GGTCCCGGCTTCGATTTCCT+TGG 0.466367 tig0022346:-34769 Msa1399240:CDS
GGCAGGAGCAAAGGCCTTGA+CGG 0.467231 tig0022346:+34807 None:intergenic
GCAGCCAAGGAAATCGAAGC+CGG 0.468475 tig0022346:+34765 None:intergenic
AAGGGTTTACCTTTGGGAAG+TGG 0.480325 tig0022346:-34535 Msa1399240:CDS
CAATGTTGCCCCATCCATCA+TGG 0.480430 tig0022346:-34377 Msa1399240:CDS
AACATCTGGGTCCACTTTGA+CGG 0.481780 tig0022346:+34714 None:intergenic
CTTGTGGCGTCAGTGTTACA+GGG 0.482574 tig0022346:-34166 Msa1399240:CDS
GACAGCAACCGCGGCTGCAG+CGG 0.482714 tig0022346:+34489 None:intergenic
CATGTTGTTGAAGCAGCCAT+TGG 0.487615 tig0022346:+34985 None:intergenic
CTTCTCTTCTGATTTCAAGC+AGG 0.492990 tig0022346:+34420 None:intergenic
CAACAATCTTATCGGAGGAC+AGG 0.494191 tig0022346:+34119 None:intergenic
AAGAAGGCTGCCATTCAAGC+TGG 0.496033 tig0022346:-34055 Msa1399240:CDS
TGTTTGAAATTAAAGGAAAG+TGG 0.496328 tig0022346:+34940 None:intergenic
CCATGTCTGGAATTCTAGCC+AGG 0.497827 tig0022346:-34197 Msa1399240:CDS
AGCGTGCACCATTGATTCCT+GGG 0.501591 tig0022346:-34090 Msa1399240:CDS
TGTCACGGATAACGAGGAAA+AGG 0.505606 tig0022346:-33987 Msa1399240:CDS
GCATCCATCCCAGGAATCAA+TGG 0.507028 tig0022346:+34082 None:intergenic
TGCCATCGACAGCGCAGCCA+AGG 0.508599 tig0022346:+34752 None:intergenic
GTCACGGATAACGAGGAAAA+GGG 0.510190 tig0022346:-33986 Msa1399240:CDS
ACCTGTTTATTCTTCCGTCA+AGG 0.511408 tig0022346:-34821 Msa1399240:CDS
GTCAAGCAGCTTGATCGCCT+TGG 0.519083 tig0022346:-33896 Msa1399240:CDS
GGCTGCGCTGTCGATGGCAT+CGG 0.519529 tig0022346:-34748 Msa1399240:CDS
ACGGAAGAATAAACAGGTGG+TGG 0.522057 tig0022346:+34826 None:intergenic
ACCGTCGCCAATCTAGGTCC+CGG 0.523818 tig0022346:-34784 Msa1399240:CDS
ACGGCATCAATTCCAAAGGT+TGG 0.527087 tig0022346:+34329 None:intergenic
AATGTTGCCCCATCCATCAT+GGG 0.527769 tig0022346:-34376 Msa1399240:CDS
GGCGACGGTGGCAGGAGCAA+AGG 0.532400 tig0022346:+34798 None:intergenic
TAGCCAGGCAGCTGCTCTTG+TGG 0.536109 tig0022346:-34182 Msa1399240:CDS
AACCCTCTTTGGAACTGCGC+TGG 0.536246 tig0022346:-34625 Msa1399240:CDS
CAGCACTGCCTTTAGAGATA+GGG 0.537569 tig0022346:-34228 Msa1399240:CDS
GCGATCAAGCTGCTTGACAT+TGG 0.539004 tig0022346:+33901 None:intergenic
CAGCCAAGGAAATCGAAGCC+GGG 0.542736 tig0022346:+34766 None:intergenic
TTCTTGACAGCATCCATCCC+AGG 0.549795 tig0022346:+34073 None:intergenic
TGCGGCATCCCTATCTCTAA+AGG 0.558659 tig0022346:+34220 None:intergenic
TGGGATGGATGCTGTCAAGA+AGG 0.561159 tig0022346:-34071 Msa1399240:CDS
GCCTTGACGGAAGAATAAAC+AGG 0.565015 tig0022346:+34820 None:intergenic
TCTCACCGGGGTGAACATCT+GGG 0.567871 tig0022346:+34701 None:intergenic
TGCACCATTGATTCCTGGGA+TGG 0.572503 tig0022346:-34086 Msa1399240:CDS
GGATTTGTTCATGTTAGGGA+AGG 0.572617 tig0022346:-34143 Msa1399240:CDS
CTGCTTGAAATCAGAAGAGA+AGG 0.573937 tig0022346:-34419 Msa1399240:CDS
ATGTGAGATGGATATCTCAC+CGG 0.575914 tig0022346:+34687 None:intergenic
AGTAGGACCAGCACCACTGA+TGG 0.581134 tig0022346:+34018 None:intergenic
TGTGAGATGGATATCTCACC+GGG 0.582722 tig0022346:+34688 None:intergenic
TACCAGCGCAGTTCCAAAGA+GGG 0.584554 tig0022346:+34623 None:intergenic
ATTTCAAGCAGGCGAGAACA+AGG 0.584734 tig0022346:+34431 None:intergenic
TCGGTAAGTGTGACGACGGA+GGG 0.586137 tig0022346:+34850 None:intergenic
AGAATAAACAGGTGGTGGGT+CGG 0.588466 tig0022346:+34831 None:intergenic
AACAATCTTATCGGAGGACA+GGG 0.598923 tig0022346:+34120 None:intergenic
TTCAACGGCATCAATTCCAA+AGG 0.604897 tig0022346:+34325 None:intergenic
ATGCTCCGCATTCGATCCGT+CGG 0.611634 tig0022346:-34577 Msa1399240:CDS
GCCGGGACCTAGATTGGCGA+CGG 0.611775 tig0022346:+34783 None:intergenic
TTGACCAGAAATATGTGAGA+TGG 0.612753 tig0022346:+34675 None:intergenic
ATGTTGCCCCATCCATCATG+GGG 0.617492 tig0022346:-34375 Msa1399240:CDS
GTTGCCCCATCCATCATGGG+GGG 0.619596 tig0022346:-34373 Msa1399240:CDS
CTTCGGGAAAAGATTGAGTG+TGG 0.620572 tig0022346:-34458 Msa1399240:CDS
CCTGGCTAGAATTCCAGACA+TGG 0.621126 tig0022346:+34197 None:intergenic
AAAGACCGACGGATCGAATG+CGG 0.626540 tig0022346:+34572 None:intergenic
TGACACTGTCTCCGTCAAAG+TGG 0.628597 tig0022346:-34725 Msa1399240:CDS
GCACATCATTGCTCAACACA+TGG 0.632675 tig0022346:-33963 Msa1399240:CDS
CGGAAGAATAAACAGGTGGT+GGG 0.638411 tig0022346:+34827 None:intergenic
AGGTTCAACAATCTTATCGG+AGG 0.639350 tig0022346:+34114 None:intergenic
CTAGAATTCCAGACATGGTG+CGG 0.639933 tig0022346:+34202 None:intergenic
GATCTCATTGACAGCAACCG+CGG 0.645517 tig0022346:+34480 None:intergenic
TCACGGATAACGAGGAAAAG+GGG 0.649323 tig0022346:-33985 Msa1399240:CDS
TCCGTGACAGCAACCGCAGT+AGG 0.650384 tig0022346:+34001 None:intergenic
TTGTGGCGTCAGTGTTACAG+GGG 0.658642 tig0022346:-34165 Msa1399240:CDS
GGTTGCTGTCACGGATAACG+AGG 0.663758 tig0022346:-33993 Msa1399240:CDS
GATCCCAAACCACTTCCCAA+AGG 0.668028 tig0022346:+34526 None:intergenic
GACGGAGGGATGCTTCTGAG+AGG 0.670246 tig0022346:+34864 None:intergenic
GTCGGTAAGTGTGACGACGG+AGG 0.671687 tig0022346:+34849 None:intergenic
ATACCAGCGCAGTTCCAAAG+AGG 0.675755 tig0022346:+34622 None:intergenic
TGTGGCGTCAGTGTTACAGG+GGG 0.680350 tig0022346:-34164 Msa1399240:CDS
GAGAGAAAGTGAAAGACCGA+CGG 0.682882 tig0022346:+34561 None:intergenic
TGCTAATAAGTCTAGCACCA+AGG 0.687849 tig0022346:+33879 None:intergenic
CAGTGGTGCTGGTCCTACTG+CGG 0.694225 tig0022346:-34014 Msa1399240:CDS
TGTTGCCCCATCCATCATGG+GGG 0.701496 tig0022346:-34374 Msa1399240:CDS
TGGGTCGGTAAGTGTGACGA+CGG 0.704433 tig0022346:+34846 None:intergenic
TTGACGGAAGAATAAACAGG+TGG 0.720068 tig0022346:+34823 None:intergenic
GTGAGATGGATATCTCACCG+GGG 0.829626 tig0022346:+34689 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCTTTTGTTTGAAATTAA+AGG + tig0022346:33914-33933 None:intergenic 15.0%
!!! ATTCTTTTGTTTGAAATTAA+AGG + tig0022346:33914-33933 None:intergenic 15.0%
!! TGTTTGAAATTAAAGGAAAG+TGG + tig0022346:33907-33926 None:intergenic 25.0%
!!! AATATAGCTGTTTTTCAGAT+GGG + tig0022346:34548-34567 None:intergenic 25.0%
!!! AAATATAGCTGTTTTTCAGA+TGG + tig0022346:34549-34568 None:intergenic 25.0%
!! TGTTTGAAATTAAAGGAAAG+TGG + tig0022346:33907-33926 None:intergenic 25.0%
!!! AATATAGCTGTTTTTCAGAT+GGG + tig0022346:34548-34567 None:intergenic 25.0%
!!! AAATATAGCTGTTTTTCAGA+TGG + tig0022346:34549-34568 None:intergenic 25.0%
ATATCCATCTCACATATTTC+TGG - tig0022346:34165-34184 Msa1399240:CDS 30.0%
!!! TGCTCTCATTTTTTTAGTTG+GGG + tig0022346:34598-34617 None:intergenic 30.0%
!!! CTGCTCTCATTTTTTTAGTT+GGG + tig0022346:34599-34618 None:intergenic 30.0%
CTTAGGTTCAACAATCTTAT+CGG + tig0022346:34736-34755 None:intergenic 30.0%
ATATCCATCTCACATATTTC+TGG - tig0022346:34165-34184 Msa1399240:CDS 30.0%
!!! TGCTCTCATTTTTTTAGTTG+GGG + tig0022346:34598-34617 None:intergenic 30.0%
!!! CTGCTCTCATTTTTTTAGTT+GGG + tig0022346:34599-34618 None:intergenic 30.0%
CTTAGGTTCAACAATCTTAT+CGG + tig0022346:34736-34755 None:intergenic 30.0%
TTGACCAGAAATATGTGAGA+TGG + tig0022346:34172-34191 None:intergenic 35.0%
TTACTGAGTTTGTGGATGTT+AGG + tig0022346:34198-34217 None:intergenic 35.0%
AACTCAGTAAAAACCCTCTT+TGG - tig0022346:34208-34227 Msa1399240:CDS 35.0%
TCTTTCACTTTCTCTCGAAA+AGG - tig0022346:34290-34309 Msa1399240:CDS 35.0%
CTTTCACTTTCTCTCGAAAA+GGG - tig0022346:34291-34310 Msa1399240:CDS 35.0%
AAATCAGAAGAGAAGGTTTC+TGG - tig0022346:34432-34451 Msa1399240:CDS 35.0%
! TTTTCAGATGGGAACTTCAA+CGG + tig0022346:34537-34556 None:intergenic 35.0%
!!! GCTGCTCTCATTTTTTTAGT+TGG + tig0022346:34600-34619 None:intergenic 35.0%
! CATGGTTCAAGCTTTTCAAA+AGG - tig0022346:34899-34918 Msa1399240:CDS 35.0%
! GTTCAAGCTTTTCAAAAGGA+AGG - tig0022346:34903-34922 Msa1399240:CDS 35.0%
TTGACCAGAAATATGTGAGA+TGG + tig0022346:34172-34191 None:intergenic 35.0%
TTACTGAGTTTGTGGATGTT+AGG + tig0022346:34198-34217 None:intergenic 35.0%
AACTCAGTAAAAACCCTCTT+TGG - tig0022346:34208-34227 Msa1399240:CDS 35.0%
TCTTTCACTTTCTCTCGAAA+AGG - tig0022346:34290-34309 Msa1399240:CDS 35.0%
CTTTCACTTTCTCTCGAAAA+GGG - tig0022346:34291-34310 Msa1399240:CDS 35.0%
AAATCAGAAGAGAAGGTTTC+TGG - tig0022346:34432-34451 Msa1399240:CDS 35.0%
! TTTTCAGATGGGAACTTCAA+CGG + tig0022346:34537-34556 None:intergenic 35.0%
!!! GCTGCTCTCATTTTTTTAGT+TGG + tig0022346:34600-34619 None:intergenic 35.0%
! CATGGTTCAAGCTTTTCAAA+AGG - tig0022346:34899-34918 Msa1399240:CDS 35.0%
! GTTCAAGCTTTTCAAAAGGA+AGG - tig0022346:34903-34922 Msa1399240:CDS 35.0%
TTGACGGAAGAATAAACAGG+TGG + tig0022346:34024-34043 None:intergenic 40.0%
ACCTGTTTATTCTTCCGTCA+AGG - tig0022346:34023-34042 Msa1399240:CDS 40.0%
ATGTGAGATGGATATCTCAC+CGG + tig0022346:34160-34179 None:intergenic 40.0%
! GAGGGTTTTTACTGAGTTTG+TGG + tig0022346:34206-34225 None:intergenic 40.0%
! CATTTTCATGGCTTCGATAG+CGG + tig0022346:34250-34269 None:intergenic 40.0%
TCTCGAAAAGGGTTTACCTT+TGG - tig0022346:34302-34321 Msa1399240:CDS 40.0%
CTCGAAAAGGGTTTACCTTT+GGG - tig0022346:34303-34322 Msa1399240:CDS 40.0%
! TTTACCTTTGGGAAGTGGTT+TGG - tig0022346:34314-34333 Msa1399240:CDS 40.0%
! TTACCTTTGGGAAGTGGTTT+GGG - tig0022346:34315-34334 Msa1399240:CDS 40.0%
GTTGCTGTCAATGAGATCTT+CGG - tig0022346:34369-34388 Msa1399240:CDS 40.0%
TTGCTGTCAATGAGATCTTC+GGG - tig0022346:34370-34389 Msa1399240:CDS 40.0%
CTTCTCTTCTGATTTCAAGC+AGG + tig0022346:34427-34446 None:intergenic 40.0%
CTGCTTGAAATCAGAAGAGA+AGG - tig0022346:34425-34444 Msa1399240:CDS 40.0%
GATTCGCAATTACCAACCTT+TGG - tig0022346:34503-34522 Msa1399240:CDS 40.0%
TTCAACGGCATCAATTCCAA+AGG + tig0022346:34522-34541 None:intergenic 40.0%
TTAGTTGGGGCTTCAAATTC+TGG + tig0022346:34585-34604 None:intergenic 40.0%
! AGGGGGATTTGTTCATGTTA+GGG - tig0022346:34697-34716 Msa1399240:CDS 40.0%
! GGATTTGTTCATGTTAGGGA+AGG - tig0022346:34701-34720 Msa1399240:CDS 40.0%
AACAATCTTATCGGAGGACA+GGG + tig0022346:34727-34746 None:intergenic 40.0%
AGGTTCAACAATCTTATCGG+AGG + tig0022346:34733-34752 None:intergenic 40.0%
TGCTAATAAGTCTAGCACCA+AGG + tig0022346:34968-34987 None:intergenic 40.0%
TTGACGGAAGAATAAACAGG+TGG + tig0022346:34024-34043 None:intergenic 40.0%
ACCTGTTTATTCTTCCGTCA+AGG - tig0022346:34023-34042 Msa1399240:CDS 40.0%
ATGTGAGATGGATATCTCAC+CGG + tig0022346:34160-34179 None:intergenic 40.0%
! GAGGGTTTTTACTGAGTTTG+TGG + tig0022346:34206-34225 None:intergenic 40.0%
! CATTTTCATGGCTTCGATAG+CGG + tig0022346:34250-34269 None:intergenic 40.0%
TCTCGAAAAGGGTTTACCTT+TGG - tig0022346:34302-34321 Msa1399240:CDS 40.0%
CTCGAAAAGGGTTTACCTTT+GGG - tig0022346:34303-34322 Msa1399240:CDS 40.0%
! TTTACCTTTGGGAAGTGGTT+TGG - tig0022346:34314-34333 Msa1399240:CDS 40.0%
! TTACCTTTGGGAAGTGGTTT+GGG - tig0022346:34315-34334 Msa1399240:CDS 40.0%
GTTGCTGTCAATGAGATCTT+CGG - tig0022346:34369-34388 Msa1399240:CDS 40.0%
TTGCTGTCAATGAGATCTTC+GGG - tig0022346:34370-34389 Msa1399240:CDS 40.0%
CTTCTCTTCTGATTTCAAGC+AGG + tig0022346:34427-34446 None:intergenic 40.0%
CTGCTTGAAATCAGAAGAGA+AGG - tig0022346:34425-34444 Msa1399240:CDS 40.0%
GATTCGCAATTACCAACCTT+TGG - tig0022346:34503-34522 Msa1399240:CDS 40.0%
TTCAACGGCATCAATTCCAA+AGG + tig0022346:34522-34541 None:intergenic 40.0%
TTAGTTGGGGCTTCAAATTC+TGG + tig0022346:34585-34604 None:intergenic 40.0%
! AGGGGGATTTGTTCATGTTA+GGG - tig0022346:34697-34716 Msa1399240:CDS 40.0%
! GGATTTGTTCATGTTAGGGA+AGG - tig0022346:34701-34720 Msa1399240:CDS 40.0%
AACAATCTTATCGGAGGACA+GGG + tig0022346:34727-34746 None:intergenic 40.0%
AGGTTCAACAATCTTATCGG+AGG + tig0022346:34733-34752 None:intergenic 40.0%
TGCTAATAAGTCTAGCACCA+AGG + tig0022346:34968-34987 None:intergenic 40.0%
!! AGAATAAACAGGTGGTGGGT+CGG + tig0022346:34016-34035 None:intergenic 45.0%
CGGAAGAATAAACAGGTGGT+GGG + tig0022346:34020-34039 None:intergenic 45.0%
ACGGAAGAATAAACAGGTGG+TGG + tig0022346:34021-34040 None:intergenic 45.0%
GCCTTGACGGAAGAATAAAC+AGG + tig0022346:34027-34046 None:intergenic 45.0%
AACATCTGGGTCCACTTTGA+CGG + tig0022346:34133-34152 None:intergenic 45.0%
TGTGAGATGGATATCTCACC+GGG + tig0022346:34159-34178 None:intergenic 45.0%
!! TCGAATGCGGAGCATTTTCA+TGG + tig0022346:34262-34281 None:intergenic 45.0%
GAGAGAAAGTGAAAGACCGA+CGG + tig0022346:34286-34305 None:intergenic 45.0%
! AAGGGTTTACCTTTGGGAAG+TGG - tig0022346:34309-34328 Msa1399240:CDS 45.0%
CTTCGGGAAAAGATTGAGTG+TGG - tig0022346:34386-34405 Msa1399240:CDS 45.0%
ATTTCAAGCAGGCGAGAACA+AGG + tig0022346:34416-34435 None:intergenic 45.0%
AATGTTGCCCCATCCATCAT+GGG - tig0022346:34468-34487 Msa1399240:CDS 45.0%
ACGGCATCAATTCCAAAGGT+TGG + tig0022346:34518-34537 None:intergenic 45.0%
! CAGCACTGCCTTTAGAGATA+GGG - tig0022346:34616-34635 Msa1399240:CDS 45.0%
CTAGAATTCCAGACATGGTG+CGG + tig0022346:34645-34664 None:intergenic 45.0%
! CAGGGGGATTTGTTCATGTT+AGG - tig0022346:34696-34715 Msa1399240:CDS 45.0%
CAACAATCTTATCGGAGGAC+AGG + tig0022346:34728-34747 None:intergenic 45.0%
TGTCACGGATAACGAGGAAA+AGG - tig0022346:34857-34876 Msa1399240:CDS 45.0%
GTCACGGATAACGAGGAAAA+GGG - tig0022346:34858-34877 Msa1399240:CDS 45.0%
TCACGGATAACGAGGAAAAG+GGG - tig0022346:34859-34878 Msa1399240:CDS 45.0%
GCACATCATTGCTCAACACA+TGG - tig0022346:34881-34900 Msa1399240:CDS 45.0%
!! AGAATAAACAGGTGGTGGGT+CGG + tig0022346:34016-34035 None:intergenic 45.0%
CGGAAGAATAAACAGGTGGT+GGG + tig0022346:34020-34039 None:intergenic 45.0%
ACGGAAGAATAAACAGGTGG+TGG + tig0022346:34021-34040 None:intergenic 45.0%
GCCTTGACGGAAGAATAAAC+AGG + tig0022346:34027-34046 None:intergenic 45.0%
AACATCTGGGTCCACTTTGA+CGG + tig0022346:34133-34152 None:intergenic 45.0%
TGTGAGATGGATATCTCACC+GGG + tig0022346:34159-34178 None:intergenic 45.0%
!! TCGAATGCGGAGCATTTTCA+TGG + tig0022346:34262-34281 None:intergenic 45.0%
GAGAGAAAGTGAAAGACCGA+CGG + tig0022346:34286-34305 None:intergenic 45.0%
! AAGGGTTTACCTTTGGGAAG+TGG - tig0022346:34309-34328 Msa1399240:CDS 45.0%
CTTCGGGAAAAGATTGAGTG+TGG - tig0022346:34386-34405 Msa1399240:CDS 45.0%
ATTTCAAGCAGGCGAGAACA+AGG + tig0022346:34416-34435 None:intergenic 45.0%
AATGTTGCCCCATCCATCAT+GGG - tig0022346:34468-34487 Msa1399240:CDS 45.0%
ACGGCATCAATTCCAAAGGT+TGG + tig0022346:34518-34537 None:intergenic 45.0%
! CAGCACTGCCTTTAGAGATA+GGG - tig0022346:34616-34635 Msa1399240:CDS 45.0%
CTAGAATTCCAGACATGGTG+CGG + tig0022346:34645-34664 None:intergenic 45.0%
! CAGGGGGATTTGTTCATGTT+AGG - tig0022346:34696-34715 Msa1399240:CDS 45.0%
CAACAATCTTATCGGAGGAC+AGG + tig0022346:34728-34747 None:intergenic 45.0%
TGTCACGGATAACGAGGAAA+AGG - tig0022346:34857-34876 Msa1399240:CDS 45.0%
GTCACGGATAACGAGGAAAA+GGG - tig0022346:34858-34877 Msa1399240:CDS 45.0%
TCACGGATAACGAGGAAAAG+GGG - tig0022346:34859-34878 Msa1399240:CDS 45.0%
GCACATCATTGCTCAACACA+TGG - tig0022346:34881-34900 Msa1399240:CDS 45.0%
TGACACTGTCTCCGTCAAAG+TGG - tig0022346:34119-34138 Msa1399240:CDS 50.0%
GTGAGATGGATATCTCACCG+GGG + tig0022346:34158-34177 None:intergenic 50.0%
TACCAGCGCAGTTCCAAAGA+GGG + tig0022346:34224-34243 None:intergenic 50.0%
ATACCAGCGCAGTTCCAAAG+AGG + tig0022346:34225-34244 None:intergenic 50.0%
AAAGACCGACGGATCGAATG+CGG + tig0022346:34275-34294 None:intergenic 50.0%
GATCCCAAACCACTTCCCAA+AGG + tig0022346:34321-34340 None:intergenic 50.0%
GATCTCATTGACAGCAACCG+CGG + tig0022346:34367-34386 None:intergenic 50.0%
CAATGTTGCCCCATCCATCA+TGG - tig0022346:34467-34486 Msa1399240:CDS 50.0%
ATGTTGCCCCATCCATCATG+GGG - tig0022346:34469-34488 Msa1399240:CDS 50.0%
!! AAAACCCCCCATGATGGATG+GGG + tig0022346:34478-34497 None:intergenic 50.0%
!! CAAAACCCCCCATGATGGAT+GGG + tig0022346:34479-34498 None:intergenic 50.0%
! ACAAAACCCCCCATGATGGA+TGG + tig0022346:34480-34499 None:intergenic 50.0%
CAACACAAAACCCCCCATGA+TGG + tig0022346:34484-34503 None:intergenic 50.0%
! GCAGCACTGCCTTTAGAGAT+AGG - tig0022346:34615-34634 Msa1399240:CDS 50.0%
TGCGGCATCCCTATCTCTAA+AGG + tig0022346:34627-34646 None:intergenic 50.0%
CCTGGCTAGAATTCCAGACA+TGG + tig0022346:34650-34669 None:intergenic 50.0%
! CCATGTCTGGAATTCTAGCC+AGG - tig0022346:34647-34666 Msa1399240:CDS 50.0%
TCTTGTGGCGTCAGTGTTAC+AGG - tig0022346:34677-34696 Msa1399240:CDS 50.0%
CTTGTGGCGTCAGTGTTACA+GGG - tig0022346:34678-34697 Msa1399240:CDS 50.0%
TTGTGGCGTCAGTGTTACAG+GGG - tig0022346:34679-34698 Msa1399240:CDS 50.0%
GGAATCAATGGTGCACGCTT+AGG + tig0022346:34753-34772 None:intergenic 50.0%
!! AAGCGTGCACCATTGATTCC+TGG - tig0022346:34753-34772 Msa1399240:CDS 50.0%
!! AGCGTGCACCATTGATTCCT+GGG - tig0022346:34754-34773 Msa1399240:CDS 50.0%
!! TGCACCATTGATTCCTGGGA+TGG - tig0022346:34758-34777 Msa1399240:CDS 50.0%
GCATCCATCCCAGGAATCAA+TGG + tig0022346:34765-34784 None:intergenic 50.0%
TTCTTGACAGCATCCATCCC+AGG + tig0022346:34774-34793 None:intergenic 50.0%
TGGGATGGATGCTGTCAAGA+AGG - tig0022346:34773-34792 Msa1399240:CDS 50.0%
AAGAAGGCTGCCATTCAAGC+TGG - tig0022346:34789-34808 Msa1399240:CDS 50.0%
! GCCATTCAAGCTGGAGCTTT+TGG - tig0022346:34798-34817 Msa1399240:CDS 50.0%
GCCAAAAGCTCCAGCTTGAA+TGG + tig0022346:34802-34821 None:intergenic 50.0%
GCGATCAAGCTGCTTGACAT+TGG + tig0022346:34946-34965 None:intergenic 50.0%
TGACACTGTCTCCGTCAAAG+TGG - tig0022346:34119-34138 Msa1399240:CDS 50.0%
GTGAGATGGATATCTCACCG+GGG + tig0022346:34158-34177 None:intergenic 50.0%
TACCAGCGCAGTTCCAAAGA+GGG + tig0022346:34224-34243 None:intergenic 50.0%
ATACCAGCGCAGTTCCAAAG+AGG + tig0022346:34225-34244 None:intergenic 50.0%
AAAGACCGACGGATCGAATG+CGG + tig0022346:34275-34294 None:intergenic 50.0%
GATCCCAAACCACTTCCCAA+AGG + tig0022346:34321-34340 None:intergenic 50.0%
GATCTCATTGACAGCAACCG+CGG + tig0022346:34367-34386 None:intergenic 50.0%
CAATGTTGCCCCATCCATCA+TGG - tig0022346:34467-34486 Msa1399240:CDS 50.0%
ATGTTGCCCCATCCATCATG+GGG - tig0022346:34469-34488 Msa1399240:CDS 50.0%
!! AAAACCCCCCATGATGGATG+GGG + tig0022346:34478-34497 None:intergenic 50.0%
!! CAAAACCCCCCATGATGGAT+GGG + tig0022346:34479-34498 None:intergenic 50.0%
! ACAAAACCCCCCATGATGGA+TGG + tig0022346:34480-34499 None:intergenic 50.0%
CAACACAAAACCCCCCATGA+TGG + tig0022346:34484-34503 None:intergenic 50.0%
! GCAGCACTGCCTTTAGAGAT+AGG - tig0022346:34615-34634 Msa1399240:CDS 50.0%
TGCGGCATCCCTATCTCTAA+AGG + tig0022346:34627-34646 None:intergenic 50.0%
CCTGGCTAGAATTCCAGACA+TGG + tig0022346:34650-34669 None:intergenic 50.0%
! CCATGTCTGGAATTCTAGCC+AGG - tig0022346:34647-34666 Msa1399240:CDS 50.0%
TCTTGTGGCGTCAGTGTTAC+AGG - tig0022346:34677-34696 Msa1399240:CDS 50.0%
CTTGTGGCGTCAGTGTTACA+GGG - tig0022346:34678-34697 Msa1399240:CDS 50.0%
TTGTGGCGTCAGTGTTACAG+GGG - tig0022346:34679-34698 Msa1399240:CDS 50.0%
GGAATCAATGGTGCACGCTT+AGG + tig0022346:34753-34772 None:intergenic 50.0%
!! AAGCGTGCACCATTGATTCC+TGG - tig0022346:34753-34772 Msa1399240:CDS 50.0%
!! AGCGTGCACCATTGATTCCT+GGG - tig0022346:34754-34773 Msa1399240:CDS 50.0%
!! TGCACCATTGATTCCTGGGA+TGG - tig0022346:34758-34777 Msa1399240:CDS 50.0%
GCATCCATCCCAGGAATCAA+TGG + tig0022346:34765-34784 None:intergenic 50.0%
TTCTTGACAGCATCCATCCC+AGG + tig0022346:34774-34793 None:intergenic 50.0%
TGGGATGGATGCTGTCAAGA+AGG - tig0022346:34773-34792 Msa1399240:CDS 50.0%
AAGAAGGCTGCCATTCAAGC+TGG - tig0022346:34789-34808 Msa1399240:CDS 50.0%
! GCCATTCAAGCTGGAGCTTT+TGG - tig0022346:34798-34817 Msa1399240:CDS 50.0%
GCCAAAAGCTCCAGCTTGAA+TGG + tig0022346:34802-34821 None:intergenic 50.0%
GCGATCAAGCTGCTTGACAT+TGG + tig0022346:34946-34965 None:intergenic 50.0%
TCGGTAAGTGTGACGACGGA+GGG + tig0022346:33997-34016 None:intergenic 55.0%
TGGGTCGGTAAGTGTGACGA+CGG + tig0022346:34001-34020 None:intergenic 55.0%
ATCGAAGCCGGGACCTAGAT+TGG + tig0022346:34070-34089 None:intergenic 55.0%
CAGCCAAGGAAATCGAAGCC+GGG + tig0022346:34081-34100 None:intergenic 55.0%
GCAGCCAAGGAAATCGAAGC+CGG + tig0022346:34082-34101 None:intergenic 55.0%
TCTCACCGGGGTGAACATCT+GGG + tig0022346:34146-34165 None:intergenic 55.0%
ATCTCACCGGGGTGAACATC+TGG + tig0022346:34147-34166 None:intergenic 55.0%
!! AACCCTCTTTGGAACTGCGC+TGG - tig0022346:34219-34238 Msa1399240:CDS 55.0%
ATGCTCCGCATTCGATCCGT+CGG - tig0022346:34267-34286 Msa1399240:CDS 55.0%
TGTTGCCCCATCCATCATGG+GGG - tig0022346:34470-34489 Msa1399240:CDS 55.0%
TGTGGCGTCAGTGTTACAGG+GGG - tig0022346:34680-34699 Msa1399240:CDS 55.0%
!! GCTTTTGGCTGCACCATCAG+TGG - tig0022346:34813-34832 Msa1399240:CDS 55.0%
AGTAGGACCAGCACCACTGA+TGG + tig0022346:34829-34848 None:intergenic 55.0%
TCCTACTGCGGTTGCTGTCA+CGG - tig0022346:34842-34861 Msa1399240:CDS 55.0%
GGTTGCTGTCACGGATAACG+AGG - tig0022346:34851-34870 Msa1399240:CDS 55.0%
GTCAAGCAGCTTGATCGCCT+TGG - tig0022346:34948-34967 Msa1399240:CDS 55.0%
TCGGTAAGTGTGACGACGGA+GGG + tig0022346:33997-34016 None:intergenic 55.0%
TGGGTCGGTAAGTGTGACGA+CGG + tig0022346:34001-34020 None:intergenic 55.0%
ATCGAAGCCGGGACCTAGAT+TGG + tig0022346:34070-34089 None:intergenic 55.0%
CAGCCAAGGAAATCGAAGCC+GGG + tig0022346:34081-34100 None:intergenic 55.0%
GCAGCCAAGGAAATCGAAGC+CGG + tig0022346:34082-34101 None:intergenic 55.0%
TCTCACCGGGGTGAACATCT+GGG + tig0022346:34146-34165 None:intergenic 55.0%
ATCTCACCGGGGTGAACATC+TGG + tig0022346:34147-34166 None:intergenic 55.0%
!! AACCCTCTTTGGAACTGCGC+TGG - tig0022346:34219-34238 Msa1399240:CDS 55.0%
ATGCTCCGCATTCGATCCGT+CGG - tig0022346:34267-34286 Msa1399240:CDS 55.0%
TGTTGCCCCATCCATCATGG+GGG - tig0022346:34470-34489 Msa1399240:CDS 55.0%
TGTGGCGTCAGTGTTACAGG+GGG - tig0022346:34680-34699 Msa1399240:CDS 55.0%
!! GCTTTTGGCTGCACCATCAG+TGG - tig0022346:34813-34832 Msa1399240:CDS 55.0%
AGTAGGACCAGCACCACTGA+TGG + tig0022346:34829-34848 None:intergenic 55.0%
TCCTACTGCGGTTGCTGTCA+CGG - tig0022346:34842-34861 Msa1399240:CDS 55.0%
GGTTGCTGTCACGGATAACG+AGG - tig0022346:34851-34870 Msa1399240:CDS 55.0%
GTCAAGCAGCTTGATCGCCT+TGG - tig0022346:34948-34967 Msa1399240:CDS 55.0%
GACGGAGGGATGCTTCTGAG+AGG + tig0022346:33983-34002 None:intergenic 60.0%
GTCGGTAAGTGTGACGACGG+AGG + tig0022346:33998-34017 None:intergenic 60.0%
GGCAGGAGCAAAGGCCTTGA+CGG + tig0022346:34040-34059 None:intergenic 60.0%
ACCGTCGCCAATCTAGGTCC+CGG - tig0022346:34060-34079 Msa1399240:CDS 60.0%
GGTCCCGGCTTCGATTTCCT+TGG - tig0022346:34075-34094 Msa1399240:CDS 60.0%
TTCCTTGGCTGCGCTGTCGA+TGG - tig0022346:34090-34109 Msa1399240:CDS 60.0%
GTGGACCCAGATGTTCACCC+CGG - tig0022346:34138-34157 Msa1399240:CDS 60.0%
GTTGCCCCATCCATCATGGG+GGG - tig0022346:34471-34490 Msa1399240:CDS 60.0%
TAGGGATGCCGCACCATGTC+TGG - tig0022346:34634-34653 Msa1399240:CDS 60.0%
TAGCCAGGCAGCTGCTCTTG+TGG - tig0022346:34662-34681 Msa1399240:CDS 60.0%
!! CAGTGGTGCTGGTCCTACTG+CGG - tig0022346:34830-34849 Msa1399240:CDS 60.0%
TCCGTGACAGCAACCGCAGT+AGG + tig0022346:34846-34865 None:intergenic 60.0%
GACGGAGGGATGCTTCTGAG+AGG + tig0022346:33983-34002 None:intergenic 60.0%
GTCGGTAAGTGTGACGACGG+AGG + tig0022346:33998-34017 None:intergenic 60.0%
GGCAGGAGCAAAGGCCTTGA+CGG + tig0022346:34040-34059 None:intergenic 60.0%
ACCGTCGCCAATCTAGGTCC+CGG - tig0022346:34060-34079 Msa1399240:CDS 60.0%
GGTCCCGGCTTCGATTTCCT+TGG - tig0022346:34075-34094 Msa1399240:CDS 60.0%
TTCCTTGGCTGCGCTGTCGA+TGG - tig0022346:34090-34109 Msa1399240:CDS 60.0%
GTGGACCCAGATGTTCACCC+CGG - tig0022346:34138-34157 Msa1399240:CDS 60.0%
GTTGCCCCATCCATCATGGG+GGG - tig0022346:34471-34490 Msa1399240:CDS 60.0%
TAGGGATGCCGCACCATGTC+TGG - tig0022346:34634-34653 Msa1399240:CDS 60.0%
TAGCCAGGCAGCTGCTCTTG+TGG - tig0022346:34662-34681 Msa1399240:CDS 60.0%
!! CAGTGGTGCTGGTCCTACTG+CGG - tig0022346:34830-34849 Msa1399240:CDS 60.0%
TCCGTGACAGCAACCGCAGT+AGG + tig0022346:34846-34865 None:intergenic 60.0%
CCTAGATTGGCGACGGTGGC+AGG + tig0022346:34057-34076 None:intergenic 65.0%
CCTGCCACCGTCGCCAATCT+AGG - tig0022346:34054-34073 Msa1399240:CDS 65.0%
GGGACCTAGATTGGCGACGG+TGG + tig0022346:34061-34080 None:intergenic 65.0%
GCCGGGACCTAGATTGGCGA+CGG + tig0022346:34064-34083 None:intergenic 65.0%
TGCCATCGACAGCGCAGCCA+AGG + tig0022346:34095-34114 None:intergenic 65.0%
GGCTGCGCTGTCGATGGCAT+CGG - tig0022346:34096-34115 Msa1399240:CDS 65.0%
ACGCCACAAGAGCAGCTGCC+TGG + tig0022346:34668-34687 None:intergenic 65.0%
GGCTGCACCATCAGTGGTGC+TGG - tig0022346:34819-34838 Msa1399240:CDS 65.0%
CCTAGATTGGCGACGGTGGC+AGG + tig0022346:34057-34076 None:intergenic 65.0%
CCTGCCACCGTCGCCAATCT+AGG - tig0022346:34054-34073 Msa1399240:CDS 65.0%
GGGACCTAGATTGGCGACGG+TGG + tig0022346:34061-34080 None:intergenic 65.0%
GCCGGGACCTAGATTGGCGA+CGG + tig0022346:34064-34083 None:intergenic 65.0%
TGCCATCGACAGCGCAGCCA+AGG + tig0022346:34095-34114 None:intergenic 65.0%
GGCTGCGCTGTCGATGGCAT+CGG - tig0022346:34096-34115 Msa1399240:CDS 65.0%
ACGCCACAAGAGCAGCTGCC+TGG + tig0022346:34668-34687 None:intergenic 65.0%
GGCTGCACCATCAGTGGTGC+TGG - tig0022346:34819-34838 Msa1399240:CDS 65.0%
GGCGACGGTGGCAGGAGCAA+AGG + tig0022346:34049-34068 None:intergenic 70.0%
GACAGCAACCGCGGCTGCAG+CGG + tig0022346:34358-34377 None:intergenic 70.0%
GGCGACGGTGGCAGGAGCAA+AGG + tig0022346:34049-34068 None:intergenic 70.0%
GACAGCAACCGCGGCTGCAG+CGG + tig0022346:34358-34377 None:intergenic 70.0%
!! TGCAGCGGCGCTGGCAGCAC+TGG + tig0022346:34343-34362 None:intergenic 75.0%
!! TGCAGCGGCGCTGGCAGCAC+TGG + tig0022346:34343-34362 None:intergenic 75.0%
! TGCCAGCGCCGCTGCAGCCG+CGG - tig0022346:34347-34366 Msa1399240:CDS 80.0%
! AACCGCGGCTGCAGCGGCGC+TGG + tig0022346:34352-34371 None:intergenic 80.0%
! TGCCAGCGCCGCTGCAGCCG+CGG - tig0022346:34347-34366 Msa1399240:CDS 80.0%
! AACCGCGGCTGCAGCGGCGC+TGG + tig0022346:34352-34371 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
tig0022346 gene 33862 35004 33862 ID=Msa1399240;Name=Msa1399240
tig0022346 mRNA 33862 35004 33862 ID=Msa1399240-mRNA-1;Parent=Msa1399240;Name=Msa1399240-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0|-1|0|1|-1|1|1|0|380
tig0022346 exon 33862 35004 33862 ID=Msa1399240-mRNA-1:exon:17123;Parent=Msa1399240-mRNA-1
tig0022346 CDS 33862 35004 33862 ID=Msa1399240-mRNA-1:cds;Parent=Msa1399240-mRNA-1
Gene Sequence

>Msa1399240

ATGGCTGCTTCAACAACATGCTTTTTTCAGTCTTCTTCATCTCCACTTTCCTTTAATTTCAAACAAAAGAATAATCAAAATATTCATATTCATATTTCAATTTTTAGAATCAGATGCTCCTCTCAGAAGCATCCCTCCGTCGTCACACTTACCGACCCACCACCTGTTTATTCTTCCGTCAAGGCCTTTGCTCCTGCCACCGTCGCCAATCTAGGTCCCGGCTTCGATTTCCTTGGCTGCGCTGTCGATGGCATCGGTGACACTGTCTCCGTCAAAGTGGACCCAGATGTTCACCCCGGTGAGATATCCATCTCACATATTTCTGGTCAAACACCTAACATCCACAAACTCAGTAAAAACCCTCTTTGGAACTGCGCTGGTATCGCCGCTATCGAAGCCATGAAAATGCTCCGCATTCGATCCGTCGGTCTTTCACTTTCTCTCGAAAAGGGTTTACCTTTGGGAAGTGGTTTGGGATCCAGTGCTGCCAGCGCCGCTGCAGCCGCGGTTGCTGTCAATGAGATCTTCGGGAAAAGATTGAGTGTGGATGACCTTGTTCTCGCCTGCTTGAAATCAGAAGAGAAGGTTTCTGGTTATCACGCAGACAATGTTGCCCCATCCATCATGGGGGGTTTTGTGTTGATTCGCAATTACCAACCTTTGGAATTGATGCCGTTGAAGTTCCCATCTGAAAAACAGCTATATTTCGTATTAGTTACACCAGAATTTGAAGCCCCAACTAAAAAAATGAGAGCAGCACTGCCTTTAGAGATAGGGATGCCGCACCATGTCTGGAATTCTAGCCAGGCAGCTGCTCTTGTGGCGTCAGTGTTACAGGGGGATTTGTTCATGTTAGGGAAGGCCCTGTCCTCCGATAAGATTGTTGAACCTAAGCGTGCACCATTGATTCCTGGGATGGATGCTGTCAAGAAGGCTGCCATTCAAGCTGGAGCTTTTGGCTGCACCATCAGTGGTGCTGGTCCTACTGCGGTTGCTGTCACGGATAACGAGGAAAAGGGGCACATCATTGCTCAACACATGGTTCAAGCTTTTCAAAAGGAAGGAAACTTGAATGCTTCTGCCAATGTCAAGCAGCTTGATCGCCTTGGTGCTAGACTTATTAGCAGCGTTCATTTAAATTGA
Protein sequence

>Msa1399240

MAASTTCFFQSSSSPLSFNFKQKNNQNIHIHISIFRIRCSSQKHPSVVTLTDPPPVYSSVKAFAPATVANLGPGFDFLGCAVDGIGDTVSVKVDPDVHPGEISISHISGQTPNIHKLSKNPLWNCAGIAAIEAMKMLRIRSVGLSLSLEKGLPLGSGLGSSAASAAAAAVAVNEIFGKRLSVDDLVLACLKSEEKVSGYHADNVAPSIMGGFVLIRNYQPLELMPLKFPSEKQLYFVLVTPEFEAPTKKMRAALPLEIGMPHHVWNSSQAAALVASVLQGDLFMLGKALSSDKIVEPKRAPLIPGMDAVKKAAIQAGAFGCTISGAGPTAVAVTDNEEKGHIIAQHMVQAFQKEGNLNASANVKQLDRLGARLISSVHLN*