AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1373140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1373140 MtrunA17_Chr7g0272611 99.281 139 1 0 1 139 1 139 6.15e-98 278
Msa1373140 MtrunA17_Chr2g0327951 82.203 118 21 0 23 140 56 173 8.70e-68 203
Msa1373140 MtrunA17_Chr4g0031041 85.455 110 16 0 23 132 15 124 3.58e-66 197
Msa1373140 MtrunA17_Chr4g0031001 81.897 116 18 1 26 141 24 136 1.04e-64 194
Msa1373140 MtrunA17_Chr4g0031651 85.047 107 16 0 26 132 24 130 2.06e-64 193
Msa1373140 MtrunA17_Chr2g0327931 80.172 116 23 0 26 141 23 138 3.59e-64 192
Msa1373140 MtrunA17_Chr8g0379311 81.034 116 19 1 26 141 24 136 9.64e-64 191
Msa1373140 MtrunA17_Chr4g0035621 75.758 132 30 1 1 132 1 130 1.07e-63 191
Msa1373140 MtrunA17_Chr4g0032301 84.112 107 17 0 26 132 24 130 2.98e-63 190
Msa1373140 MtrunA17_Chr7g0268831 71.970 132 33 1 1 132 1 128 5.45e-62 186
Msa1373140 MtrunA17_Chr7g0273351 73.810 126 31 1 7 132 2 125 7.01e-61 184
Msa1373140 MtrunA17_Chr7g0273331 73.810 126 31 1 7 132 58 181 4.26e-60 184
Msa1373140 MtrunA17_Chr7g0259151 72.727 132 34 2 6 137 3 132 8.04e-60 181
Msa1373140 MtrunA17_Chr2g0319191 77.679 112 25 0 21 132 11 122 3.56e-59 179
Msa1373140 MtrunA17_Chr2g0319181 78.899 109 23 0 24 132 14 122 1.07e-58 178
Msa1373140 MtrunA17_Chr6g0449661 79.661 118 13 3 26 140 85 194 1.61e-58 180
Msa1373140 MtrunA17_Chr3g0119971 78.632 117 14 3 27 140 81 189 1.05e-57 177
Msa1373140 MtrunA17_Chr7g0273341 66.667 123 28 3 10 132 44 153 1.83e-47 150
Msa1373140 MtrunA17_Chr4g0031031 83.784 74 12 0 47 120 10 83 3.32e-39 128
Msa1373140 MtrunA17_Chr1g0148961 54.032 124 55 2 10 132 10 132 3.31e-37 124
Msa1373140 MtrunA17_Chr1g0148951 54.032 124 55 2 10 132 10 132 3.31e-37 124
Msa1373140 MtrunA17_Chr1g0211651 53.782 119 53 2 13 130 10 127 2.54e-36 121
Msa1373140 MtrunA17_Chr1g0184901 57.692 104 42 2 28 130 28 130 1.27e-34 117
Msa1373140 MtrunA17_Chr4g0024701 46.667 105 36 2 26 130 26 110 1.87e-22 85.5
Msa1373140 MtrunA17_Chr1g0183701 60.938 64 25 0 69 132 30 93 1.74e-19 77.8
Msa1373140 MtrunA17_Chr4g0000531 68.889 45 14 0 87 131 5 49 1.68e-14 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1373140 AT5G27670.1 78.261 138 30 0 4 141 3 140 8.72e-79 229
Msa1373140 AT5G02560.1 81.356 118 22 0 23 140 21 138 1.74e-66 199
Msa1373140 AT5G59870.1 72.662 139 33 2 7 140 3 141 2.47e-65 196
Msa1373140 AT5G02560.2 67.606 142 22 1 23 140 21 162 1.26e-60 185
Msa1373140 AT1G51060.1 74.167 120 31 0 21 140 11 130 1.74e-60 183
Msa1373140 AT1G08880.1 72.222 126 31 1 10 131 2 127 2.55e-59 180
Msa1373140 AT4G27230.2 72.500 120 33 0 21 140 11 130 8.05e-59 179
Msa1373140 AT4G27230.1 72.500 120 33 0 21 140 11 130 8.05e-59 179
Msa1373140 AT5G54640.1 75.862 116 28 0 21 136 11 126 8.42e-59 178
Msa1373140 AT1G54690.1 70.866 127 33 1 10 132 2 128 9.92e-59 179
Msa1373140 AT3G20670.1 71.901 121 34 0 21 141 11 131 2.13e-58 177
Msa1373140 AT2G38810.1 57.143 112 44 3 23 132 25 134 4.68e-36 121
Msa1373140 AT2G38810.4 57.143 112 44 3 23 132 25 134 4.68e-36 121
Msa1373140 AT2G38810.3 57.143 112 44 3 23 132 25 134 4.68e-36 121
Msa1373140 AT2G38810.2 57.143 112 44 3 23 132 25 134 4.68e-36 121
Msa1373140 AT1G52740.1 56.190 105 44 2 27 130 27 130 1.02e-34 117
Msa1373140 AT3G54560.1 56.481 108 43 3 27 132 29 134 7.43e-34 115
Msa1373140 AT3G54560.2 56.481 108 43 3 27 132 29 134 7.43e-34 115
Msa1373140 AT4G13570.1 45.161 93 47 3 34 124 28 118 1.15e-19 79.0
Msa1373140 AT4G13570.2 40.566 106 59 3 21 124 21 124 1.26e-19 79.0

Find 48 sgRNAs with CRISPR-Local

Find 131 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAACACAGGGTTAATATT+TGG 0.220863 tig0019363:-109575 None:intergenic
AGGAATGTGAAATTGGAATT+TGG 0.240114 tig0019363:-109053 None:intergenic
ATGTAGTGGTTTGTTAAATA+TGG 0.242903 tig0019363:+109768 Msa1373140:three_prime_UTR
GTAAAATAGGAATGTGAAAT+TGG 0.292099 tig0019363:-109060 None:intergenic
ATGGAGGGAATGAGTAAAAT+AGG 0.297025 tig0019363:-109073 None:intergenic
GGGGAATTGAAGACCGGCTT+TGG 0.356697 tig0019363:-109222 None:intergenic
GTGAGGAACGATGATGAATT+AGG 0.390738 tig0019363:+109509 Msa1373140:CDS
GGTTGTAGTTGTTGTGGTGG+TGG 0.425621 tig0019363:-109141 None:intergenic
AACCTAGCTATACGACCAAC+GGG 0.429162 tig0019363:-109242 None:intergenic
TCTTCCACCGGCACCTTTGG+TGG 0.442255 tig0019363:-109162 None:intergenic
TGTAGTTGTTGTGGTGGTGG+TGG 0.444305 tig0019363:-109138 None:intergenic
TATTCTCAGCGTGTTGGCAC+TGG 0.452088 tig0019363:+109281 Msa1373140:CDS
TTTAGATCTAACTATTTCGT+TGG 0.454793 tig0019363:+109738 Msa1373140:three_prime_UTR
TGTAATGTAGGTTCTTGAAT+TGG 0.463460 tig0019363:+109424 Msa1373140:intron
ACAGCAAGGCACACGTGTCT+AGG 0.469171 tig0019363:-109488 None:intergenic
ATGTAGGTTCTTGAATTGGC+TGG 0.481956 tig0019363:+109428 Msa1373140:intron
TTTATTGAAGTGCATTTACA+TGG 0.483044 tig0019363:+109681 Msa1373140:three_prime_UTR
AAAGGTGCCGGTGGAAGAAA+AGG 0.504433 tig0019363:+109167 Msa1373140:CDS
TGTAGGTTCTTGAATTGGCT+GGG 0.516295 tig0019363:+109429 Msa1373140:intron
AGAACGGCGGCGAGGTAGAT+CGG 0.519256 tig0019363:-109308 None:intergenic
TCCCCGTTGGTCGTATAGCT+AGG 0.519588 tig0019363:+109240 Msa1373140:CDS
GAATTGAAGACCGGCTTTGG+AGG 0.520441 tig0019363:-109219 None:intergenic
GAATTAGGAAAATTGCTTCA+AGG 0.523033 tig0019363:+109524 Msa1373140:CDS
CAGCAAGGCACACGTGTCTA+GGG 0.534173 tig0019363:-109487 None:intergenic
TTTGGTGGTTGTAGTTGTTG+TGG 0.545704 tig0019363:-109147 None:intergenic
GGTGGTTGTAGTTGTTGTGG+TGG 0.550499 tig0019363:-109144 None:intergenic
ATAGCTAGGTTCATGAAGAA+AGG 0.556055 tig0019363:+109254 Msa1373140:CDS
GTGGAAGAAAAGGCGGCGAA+AGG 0.564273 tig0019363:+109177 Msa1373140:CDS
TCGTTGGATGATAAATGTAG+TGG 0.571863 tig0019363:+109754 Msa1373140:three_prime_UTR
GTTTCAAAATCCTCCAAAGC+CGG 0.573530 tig0019363:+109209 Msa1373140:CDS
ACTACAACCACCAAAGGTGC+CGG 0.576198 tig0019363:+109155 Msa1373140:CDS
GCCGGTCTTCAATTCCCCGT+TGG 0.577353 tig0019363:+109227 Msa1373140:CDS
GCCAAAGAAAACCGTTGCTG+CGG 0.590680 tig0019363:+109598 Msa1373140:CDS
TCTCGAATATCTCGCTGCCG+AGG 0.591297 tig0019363:+109328 Msa1373140:CDS
GAACCTAGCTATACGACCAA+CGG 0.599597 tig0019363:-109243 None:intergenic
GGTCGCTATTCTCAGCGTGT+TGG 0.618289 tig0019363:+109275 Msa1373140:CDS
ACCAACGGGGAATTGAAGAC+CGG 0.624424 tig0019363:-109228 None:intergenic
ACAACAACTACAACCACCAA+AGG 0.640375 tig0019363:+109149 Msa1373140:CDS
CATCATCGTTCCTCACAGCA+AGG 0.645037 tig0019363:-109502 None:intergenic
ACAACCACCAAAGGTGCCGG+TGG 0.649704 tig0019363:+109158 Msa1373140:CDS
GGTGTGACAATTGCTAGTGG+TGG 0.656244 tig0019363:+109545 Msa1373140:CDS
GACACGTGTGCCTTGCTGTG+AGG 0.657788 tig0019363:+109492 Msa1373140:CDS
GGCAGCGAGATATTCGAGAA+CGG 0.664119 tig0019363:-109324 None:intergenic
CAAGGTGTGACAATTGCTAG+TGG 0.675402 tig0019363:+109542 Msa1373140:CDS
GGTGCCGGTGGAAGAAAAGG+CGG 0.678900 tig0019363:+109170 Msa1373140:CDS
GATATTCGAGAACGGCGGCG+AGG 0.697780 tig0019363:-109316 None:intergenic
AGCGAGATATTCGAGAACGG+CGG 0.709556 tig0019363:-109321 None:intergenic
ACCTAGCTATACGACCAACG+GGG 0.792421 tig0019363:-109241 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGTAATTAATTAATGTTT+TGG + tig0019363:109394-109413 Msa1373140:intron 10.0%
!!! TTTGTAATTAATTAATGTTT+TGG + tig0019363:109394-109413 Msa1373140:intron 10.0%
!! AAGCGAAATTCATAATTAAT+CGG + tig0019363:109369-109388 Msa1373140:intron 20.0%
!! AAGCGAAATTCATAATTAAT+CGG + tig0019363:109369-109388 Msa1373140:intron 20.0%
! GTAAAATAGGAATGTGAAAT+TGG - tig0019363:109063-109082 None:intergenic 25.0%
!!! GAATTTCGCTTTGAAATATA+CGG - tig0019363:109360-109379 None:intergenic 25.0%
! TTTATTGAAGTGCATTTACA+TGG + tig0019363:109681-109700 Msa1373140:three_prime_UTR 25.0%
! TTTAGATCTAACTATTTCGT+TGG + tig0019363:109738-109757 Msa1373140:three_prime_UTR 25.0%
!!! ATGTAGTGGTTTGTTAAATA+TGG + tig0019363:109768-109787 Msa1373140:three_prime_UTR 25.0%
! GTAAAATAGGAATGTGAAAT+TGG - tig0019363:109063-109082 None:intergenic 25.0%
!!! GAATTTCGCTTTGAAATATA+CGG - tig0019363:109360-109379 None:intergenic 25.0%
! TTTATTGAAGTGCATTTACA+TGG + tig0019363:109681-109700 Msa1373140:three_prime_UTR 25.0%
! TTTAGATCTAACTATTTCGT+TGG + tig0019363:109738-109757 Msa1373140:three_prime_UTR 25.0%
!!! ATGTAGTGGTTTGTTAAATA+TGG + tig0019363:109768-109787 Msa1373140:three_prime_UTR 25.0%
AGGAATGTGAAATTGGAATT+TGG - tig0019363:109056-109075 None:intergenic 30.0%
!!! TTTTTTTTGCGATCCGAAAA+TGG - tig0019363:109095-109114 None:intergenic 30.0%
GAAATATACGGAAAATACCT+CGG - tig0019363:109348-109367 None:intergenic 30.0%
TTTGGTATGTGATGTAATGT+AGG + tig0019363:109412-109431 Msa1373140:intron 30.0%
TGTAATGTAGGTTCTTGAAT+TGG + tig0019363:109424-109443 Msa1373140:intron 30.0%
GAATTAGGAAAATTGCTTCA+AGG + tig0019363:109524-109543 Msa1373140:CDS 30.0%
AACAACACAGGGTTAATATT+TGG - tig0019363:109578-109597 None:intergenic 30.0%
AGGAATGTGAAATTGGAATT+TGG - tig0019363:109056-109075 None:intergenic 30.0%
!!! TTTTTTTTGCGATCCGAAAA+TGG - tig0019363:109095-109114 None:intergenic 30.0%
GAAATATACGGAAAATACCT+CGG - tig0019363:109348-109367 None:intergenic 30.0%
TTTGGTATGTGATGTAATGT+AGG + tig0019363:109412-109431 Msa1373140:intron 30.0%
TGTAATGTAGGTTCTTGAAT+TGG + tig0019363:109424-109443 Msa1373140:intron 30.0%
GAATTAGGAAAATTGCTTCA+AGG + tig0019363:109524-109543 Msa1373140:CDS 30.0%
AACAACACAGGGTTAATATT+TGG - tig0019363:109578-109597 None:intergenic 30.0%
ATGGAGGGAATGAGTAAAAT+AGG - tig0019363:109076-109095 None:intergenic 35.0%
! TTACTCATTCCCTCCATTTT+CGG + tig0019363:109079-109098 Msa1373140:five_prime_UTR 35.0%
ATAGCTAGGTTCATGAAGAA+AGG + tig0019363:109254-109273 Msa1373140:CDS 35.0%
! TTTTCTTTGGCAACAACACA+GGG - tig0019363:109589-109608 None:intergenic 35.0%
TCGTTGGATGATAAATGTAG+TGG + tig0019363:109754-109773 Msa1373140:three_prime_UTR 35.0%
GGATGAAGTGTGATAAAGAT+TGG - tig0019363:109035-109054 None:intergenic 35.0%
ATGGAGGGAATGAGTAAAAT+AGG - tig0019363:109076-109095 None:intergenic 35.0%
! TTACTCATTCCCTCCATTTT+CGG + tig0019363:109079-109098 Msa1373140:five_prime_UTR 35.0%
ATAGCTAGGTTCATGAAGAA+AGG + tig0019363:109254-109273 Msa1373140:CDS 35.0%
! TTTTCTTTGGCAACAACACA+GGG - tig0019363:109589-109608 None:intergenic 35.0%
TCGTTGGATGATAAATGTAG+TGG + tig0019363:109754-109773 Msa1373140:three_prime_UTR 35.0%
! TTTTGCGATCCGAAAATGGA+GGG - tig0019363:109091-109110 None:intergenic 40.0%
! TTTTTGCGATCCGAAAATGG+AGG - tig0019363:109092-109111 None:intergenic 40.0%
! TTTGGTGGTTGTAGTTGTTG+TGG - tig0019363:109150-109169 None:intergenic 40.0%
ACAACAACTACAACCACCAA+AGG + tig0019363:109149-109168 Msa1373140:CDS 40.0%
!!! GGCTTTGGAGGATTTTGAAA+CGG - tig0019363:109210-109229 None:intergenic 40.0%
GTTTCAAAATCCTCCAAAGC+CGG + tig0019363:109209-109228 Msa1373140:CDS 40.0%
ATGTAGGTTCTTGAATTGGC+TGG + tig0019363:109428-109447 Msa1373140:intron 40.0%
TGTAGGTTCTTGAATTGGCT+GGG + tig0019363:109429-109448 Msa1373140:intron 40.0%
GTGAGGAACGATGATGAATT+AGG + tig0019363:109509-109528 Msa1373140:CDS 40.0%
! GTTTTCTTTGGCAACAACAC+AGG - tig0019363:109590-109609 None:intergenic 40.0%
! TTTTGCGATCCGAAAATGGA+GGG - tig0019363:109091-109110 None:intergenic 40.0%
! TTTTTGCGATCCGAAAATGG+AGG - tig0019363:109092-109111 None:intergenic 40.0%
! TTTGGTGGTTGTAGTTGTTG+TGG - tig0019363:109150-109169 None:intergenic 40.0%
ACAACAACTACAACCACCAA+AGG + tig0019363:109149-109168 Msa1373140:CDS 40.0%
!!! GGCTTTGGAGGATTTTGAAA+CGG - tig0019363:109210-109229 None:intergenic 40.0%
GTTTCAAAATCCTCCAAAGC+CGG + tig0019363:109209-109228 Msa1373140:CDS 40.0%
ATGTAGGTTCTTGAATTGGC+TGG + tig0019363:109428-109447 Msa1373140:intron 40.0%
TGTAGGTTCTTGAATTGGCT+GGG + tig0019363:109429-109448 Msa1373140:intron 40.0%
GTGAGGAACGATGATGAATT+AGG + tig0019363:109509-109528 Msa1373140:CDS 40.0%
! GTTTTCTTTGGCAACAACAC+AGG - tig0019363:109590-109609 None:intergenic 40.0%
AACCTAGCTATACGACCAAC+GGG - tig0019363:109245-109264 None:intergenic 45.0%
GAACCTAGCTATACGACCAA+CGG - tig0019363:109246-109265 None:intergenic 45.0%
CAAGGTGTGACAATTGCTAG+TGG + tig0019363:109542-109561 Msa1373140:CDS 45.0%
! AGTAGTTTTCTCCGCAGCAA+CGG - tig0019363:109612-109631 None:intergenic 45.0%
AACCTAGCTATACGACCAAC+GGG - tig0019363:109245-109264 None:intergenic 45.0%
GAACCTAGCTATACGACCAA+CGG - tig0019363:109246-109265 None:intergenic 45.0%
CAAGGTGTGACAATTGCTAG+TGG + tig0019363:109542-109561 Msa1373140:CDS 45.0%
! AGTAGTTTTCTCCGCAGCAA+CGG - tig0019363:109612-109631 None:intergenic 45.0%
!! AAAAAAAAAAAAAAAAACAA+TGG + tig0019363:109112-109131 Msa1373140:exon 5.0%
!! AAAAAAAAAAAAAAAAACAA+TGG + tig0019363:109112-109131 Msa1373140:exon 5.0%
!! TGTAGTTGTTGTGGTGGTGG+TGG - tig0019363:109141-109160 None:intergenic 50.0%
GGTTGTAGTTGTTGTGGTGG+TGG - tig0019363:109144-109163 None:intergenic 50.0%
GGTGGTTGTAGTTGTTGTGG+TGG - tig0019363:109147-109166 None:intergenic 50.0%
! ACTACAACCACCAAAGGTGC+CGG + tig0019363:109155-109174 Msa1373140:CDS 50.0%
! TTTTCTTCCACCGGCACCTT+TGG - tig0019363:109168-109187 None:intergenic 50.0%
!! AAAGGTGCCGGTGGAAGAAA+AGG + tig0019363:109167-109186 Msa1373140:CDS 50.0%
! GAATTGAAGACCGGCTTTGG+AGG - tig0019363:109222-109241 None:intergenic 50.0%
ACCAACGGGGAATTGAAGAC+CGG - tig0019363:109231-109250 None:intergenic 50.0%
ACCTAGCTATACGACCAACG+GGG - tig0019363:109244-109263 None:intergenic 50.0%
! TATTCTCAGCGTGTTGGCAC+TGG + tig0019363:109281-109300 Msa1373140:CDS 50.0%
AGCGAGATATTCGAGAACGG+CGG - tig0019363:109324-109343 None:intergenic 50.0%
GGCAGCGAGATATTCGAGAA+CGG - tig0019363:109327-109346 None:intergenic 50.0%
CATCATCGTTCCTCACAGCA+AGG - tig0019363:109505-109524 None:intergenic 50.0%
GGTGTGACAATTGCTAGTGG+TGG + tig0019363:109545-109564 Msa1373140:CDS 50.0%
GCCAAAGAAAACCGTTGCTG+CGG + tig0019363:109598-109617 Msa1373140:CDS 50.0%
! TCCGCAGCAACGGTTTTCTT+TGG - tig0019363:109602-109621 None:intergenic 50.0%
!! TGTAGTTGTTGTGGTGGTGG+TGG - tig0019363:109141-109160 None:intergenic 50.0%
GGTTGTAGTTGTTGTGGTGG+TGG - tig0019363:109144-109163 None:intergenic 50.0%
GGTGGTTGTAGTTGTTGTGG+TGG - tig0019363:109147-109166 None:intergenic 50.0%
! ACTACAACCACCAAAGGTGC+CGG + tig0019363:109155-109174 Msa1373140:CDS 50.0%
! TTTTCTTCCACCGGCACCTT+TGG - tig0019363:109168-109187 None:intergenic 50.0%
!! AAAGGTGCCGGTGGAAGAAA+AGG + tig0019363:109167-109186 Msa1373140:CDS 50.0%
! GAATTGAAGACCGGCTTTGG+AGG - tig0019363:109222-109241 None:intergenic 50.0%
ACCAACGGGGAATTGAAGAC+CGG - tig0019363:109231-109250 None:intergenic 50.0%
ACCTAGCTATACGACCAACG+GGG - tig0019363:109244-109263 None:intergenic 50.0%
! TATTCTCAGCGTGTTGGCAC+TGG + tig0019363:109281-109300 Msa1373140:CDS 50.0%
AGCGAGATATTCGAGAACGG+CGG - tig0019363:109324-109343 None:intergenic 50.0%
GGCAGCGAGATATTCGAGAA+CGG - tig0019363:109327-109346 None:intergenic 50.0%
CATCATCGTTCCTCACAGCA+AGG - tig0019363:109505-109524 None:intergenic 50.0%
GGTGTGACAATTGCTAGTGG+TGG + tig0019363:109545-109564 Msa1373140:CDS 50.0%
GCCAAAGAAAACCGTTGCTG+CGG + tig0019363:109598-109617 Msa1373140:CDS 50.0%
! TCCGCAGCAACGGTTTTCTT+TGG - tig0019363:109602-109621 None:intergenic 50.0%
! TTCGCCGCCTTTTCTTCCAC+CGG - tig0019363:109177-109196 None:intergenic 55.0%
GTGGAAGAAAAGGCGGCGAA+AGG + tig0019363:109177-109196 Msa1373140:CDS 55.0%
! GGGGAATTGAAGACCGGCTT+TGG - tig0019363:109225-109244 None:intergenic 55.0%
TCCCCGTTGGTCGTATAGCT+AGG + tig0019363:109240-109259 Msa1373140:CDS 55.0%
GGTCGCTATTCTCAGCGTGT+TGG + tig0019363:109275-109294 Msa1373140:CDS 55.0%
TCTCGAATATCTCGCTGCCG+AGG + tig0019363:109328-109347 Msa1373140:CDS 55.0%
CAGCAAGGCACACGTGTCTA+GGG - tig0019363:109490-109509 None:intergenic 55.0%
ACAGCAAGGCACACGTGTCT+AGG - tig0019363:109491-109510 None:intergenic 55.0%
! TTCGCCGCCTTTTCTTCCAC+CGG - tig0019363:109177-109196 None:intergenic 55.0%
GTGGAAGAAAAGGCGGCGAA+AGG + tig0019363:109177-109196 Msa1373140:CDS 55.0%
! GGGGAATTGAAGACCGGCTT+TGG - tig0019363:109225-109244 None:intergenic 55.0%
TCCCCGTTGGTCGTATAGCT+AGG + tig0019363:109240-109259 Msa1373140:CDS 55.0%
GGTCGCTATTCTCAGCGTGT+TGG + tig0019363:109275-109294 Msa1373140:CDS 55.0%
TCTCGAATATCTCGCTGCCG+AGG + tig0019363:109328-109347 Msa1373140:CDS 55.0%
CAGCAAGGCACACGTGTCTA+GGG - tig0019363:109490-109509 None:intergenic 55.0%
ACAGCAAGGCACACGTGTCT+AGG - tig0019363:109491-109510 None:intergenic 55.0%
!! ACAACCACCAAAGGTGCCGG+TGG + tig0019363:109158-109177 Msa1373140:CDS 60.0%
TCTTCCACCGGCACCTTTGG+TGG - tig0019363:109165-109184 None:intergenic 60.0%
!! GGTGCCGGTGGAAGAAAAGG+CGG + tig0019363:109170-109189 Msa1373140:CDS 60.0%
GCCGGTCTTCAATTCCCCGT+TGG + tig0019363:109227-109246 Msa1373140:CDS 60.0%
AGAACGGCGGCGAGGTAGAT+CGG - tig0019363:109311-109330 None:intergenic 60.0%
GATATTCGAGAACGGCGGCG+AGG - tig0019363:109319-109338 None:intergenic 60.0%
! GACACGTGTGCCTTGCTGTG+AGG + tig0019363:109492-109511 Msa1373140:CDS 60.0%
!! ACAACCACCAAAGGTGCCGG+TGG + tig0019363:109158-109177 Msa1373140:CDS 60.0%
TCTTCCACCGGCACCTTTGG+TGG - tig0019363:109165-109184 None:intergenic 60.0%
!! GGTGCCGGTGGAAGAAAAGG+CGG + tig0019363:109170-109189 Msa1373140:CDS 60.0%
GCCGGTCTTCAATTCCCCGT+TGG + tig0019363:109227-109246 Msa1373140:CDS 60.0%
AGAACGGCGGCGAGGTAGAT+CGG - tig0019363:109311-109330 None:intergenic 60.0%
GATATTCGAGAACGGCGGCG+AGG - tig0019363:109319-109338 None:intergenic 60.0%
! GACACGTGTGCCTTGCTGTG+AGG + tig0019363:109492-109511 Msa1373140:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0019363 gene 109033 109829 109033 ID=Msa1373140;Name=Msa1373140
tig0019363 mRNA 109033 109829 109033 ID=Msa1373140-mRNA-1;Parent=Msa1373140;Name=Msa1373140-mRNA-1;_AED=0.39;_eAED=0.39;_QI=98|1|1|1|1|1|2|189|141
tig0019363 exon 109033 109349 109033 ID=Msa1373140-mRNA-1:exon:3105;Parent=Msa1373140-mRNA-1
tig0019363 exon 109434 109829 109434 ID=Msa1373140-mRNA-1:exon:3106;Parent=Msa1373140-mRNA-1
tig0019363 five_prime_UTR 109033 109130 109033 ID=Msa1373140-mRNA-1:five_prime_utr;Parent=Msa1373140-mRNA-1
tig0019363 CDS 109131 109349 109131 ID=Msa1373140-mRNA-1:cds;Parent=Msa1373140-mRNA-1
tig0019363 CDS 109434 109640 109434 ID=Msa1373140-mRNA-1:cds;Parent=Msa1373140-mRNA-1
tig0019363 three_prime_UTR 109641 109829 109641 ID=Msa1373140-mRNA-1:three_prime_utr;Parent=Msa1373140-mRNA-1
Gene Sequence

>Msa1373140

ATGGATTCCACCACCACCACAACAACTACAACCACCAAAGGTGCCGGTGGAAGAAAAGGCGGCGAAAGGAAGAAAGCCGTTTCAAAATCCTCCAAAGCCGGTCTTCAATTCCCCGTTGGTCGTATAGCTAGGTTCATGAAGAAAGGTCGCTATTCTCAGCGTGTTGGCACTGGTGCTCCGATCTACCTCGCCGCCGTTCTCGAATATCTCGCTGCCGAGGTTCTTGAATTGGCTGGGAATGCTGCAAGAGATAACAAGAAGAACAGAATTAACCCTAGACACGTGTGCCTTGCTGTGAGGAACGATGATGAATTAGGAAAATTGCTTCAAGGTGTGACAATTGCTAGTGGTGGAGTTTTGCCAAATATTAACCCTGTGTTGTTGCCAAAGAAAACCGTTGCTGCGGAGAAAACTACTAAGAAATAA
Protein sequence

>Msa1373140

MDSTTTTTTTTTKGAGGRKGGERKKAVSKSSKAGLQFPVGRIARFMKKGRYSQRVGTGAPIYLAAVLEYLAAEVLELAGNAARDNKKNRINPRHVCLAVRNDDELGKLLQGVTIASGGVLPNINPVLLPKKTVAAEKTTKK*