AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0488800

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0488800 MtrunA17_Chr3g0138581 98.889 90 1 0 1 90 101 190 4.39e-57 179
Msa0488800 MtrunA17_Chr5g0444731 59.091 88 36 0 1 88 85 172 9.16e-27 100
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0488800 AT3G24350.1 73.626 91 22 1 2 90 95 185 1.09e-38 132
Msa0488800 AT3G24350.2 73.626 91 22 1 2 90 109 199 1.49e-38 132
Msa0488800 AT5G05760.1 58.824 85 35 0 1 85 83 167 1.66e-27 102

Find 91 sgRNAs with CRISPR-Local

Find 194 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTAATTAGAATTGGAATT+TGG 0.134767 3_4:-8934263 None:intergenic
GTGAATTCCATAACCGATTT+TGG 0.158089 3_4:-8934517 None:intergenic
ACGAAGGAGGTAACGAATTT+AGG 0.184062 3_4:-8934185 None:intergenic
AAAATTATGAAAACTTTGTT+TGG 0.288021 3_4:-8934217 None:intergenic
GAGGTAACGAATTTAGGATT+TGG 0.313188 3_4:-8934179 None:intergenic
TAGGTTTATGCATACAAATT+AGG 0.320658 3_4:+8936567 Msa0488800:three_prime_UTR
TTCCAATTCTAATTAGATCT+AGG 0.323460 3_4:+8934268 Msa0488800:five_prime_UTR
TCTAATTAGAATTGGAATTT+GGG 0.328445 3_4:-8934262 None:intergenic
ACCCTAGATCTAATTAGAAT+TGG 0.351555 3_4:-8934270 None:intergenic
TTGAGTTTCTAAGCAAATAA+TGG 0.358465 3_4:+8936667 Msa0488800:three_prime_UTR
AATCGAAGAGCTTCCAAAAT+CGG 0.371732 3_4:+8934504 Msa0488800:five_prime_UTR
AATTCTTGAATCTCCATTGT+TGG 0.374772 3_4:-8935631 None:intergenic
GAGCAGAGAGAGAGAATGGT+TGG 0.377896 3_4:-8934035 None:intergenic
CCACTAGCCCATGGAGGTGC+AGG 0.392951 3_4:-8936060 None:intergenic
GCACTGGTGGTGGCTCCGTT+TGG 0.394889 3_4:-8934408 None:intergenic
ATATCAAGTTATTGTGTACT+TGG 0.396196 3_4:+8935444 Msa0488800:five_prime_UTR
AGAAACTATCAAATGTTTCC+AGG 0.401698 3_4:+8936630 Msa0488800:three_prime_UTR
TTGAAGAAATCAGTTTCATC+TGG 0.402299 3_4:+8934378 Msa0488800:five_prime_UTR
CGCTCTTAACTCTGCTGTTG+TGG 0.403619 3_4:+8935681 Msa0488800:CDS
AAATTATGAAAACTTTGTTT+GGG 0.405588 3_4:-8934216 None:intergenic
TGATTTGAACTACATCTTTG+AGG 0.407478 3_4:-8934301 None:intergenic
TTAATTTATTGAGTCCTGAT+AGG 0.407510 3_4:+8933924 None:intergenic
CCTGCACCTCCATGGGCTAG+TGG 0.415756 3_4:+8936060 Msa0488800:three_prime_UTR
AGAGCTTCCAAAATCGGTTA+TGG 0.421078 3_4:+8934510 Msa0488800:five_prime_UTR
CTAGTGCCCCTGCACCTCCA+TGG 0.421904 3_4:+8936052 Msa0488800:three_prime_UTR
AAGAACATCTTTGAATTCCT+TGG 0.423257 3_4:-8935815 None:intergenic
TGGAAAGTTTCTGAGAGGTT+TGG 0.428300 3_4:-8934539 None:intergenic
TCCAATTCTAATTAGATCTA+GGG 0.429405 3_4:+8934269 Msa0488800:five_prime_UTR
TAGGAGGGTGTTTGCCACAT+GGG 0.438349 3_4:-8933955 None:intergenic
TGCTCATCAATCGTGTCTTT+AGG 0.440887 3_4:-8935789 None:intergenic
TCAAGGATCTTTAGATGCCC+TGG 0.444067 3_4:-8936648 None:intergenic
GAGATTACATTTGGCGAGCT+TGG 0.452819 3_4:-8934559 None:intergenic
TAACAAACAATCATGAAAGC+TGG 0.454594 3_4:+8934145 Msa0488800:five_prime_UTR
ACGAATTTAGGATTTGGAAA+TGG 0.454954 3_4:-8934173 None:intergenic
TAGAGAGCAGAGAGAGAGAA+TGG 0.456700 3_4:-8934039 None:intergenic
TGGAAAGAGCTGTGGGAGTG+TGG 0.457370 3_4:-8934015 None:intergenic
AGATCGAGGATCGTCGGATC+TGG 0.457412 3_4:-8934457 None:intergenic
ATAATACCAAGCAAAGGTTC+AGG 0.460003 3_4:+8937042 Msa0488800:three_prime_UTR
TGCTATTAGCTGGTGTTGGT+TGG 0.476257 3_4:+8936590 Msa0488800:three_prime_UTR
AGGAGGGTGTTTGCCACATG+GGG 0.478437 3_4:-8933954 None:intergenic
AGAATGGTTGGAAAGAGCTG+TGG 0.483595 3_4:-8934023 None:intergenic
ACAAATTAGGTGCTATTAGC+TGG 0.485147 3_4:+8936580 Msa0488800:three_prime_UTR
CTGCACCTCCATGGGCTAGT+GGG 0.490575 3_4:+8936061 Msa0488800:three_prime_UTR
TAGTGCCCCTGCACCTCCAT+GGG 0.492305 3_4:+8936053 Msa0488800:three_prime_UTR
AATTTGGGATCGATTGATTG+AGG 0.492483 3_4:-8934247 None:intergenic
TGTTCTTACCATGCGAATTG+AGG 0.494197 3_4:+8935831 Msa0488800:CDS
TGTACTTGGAGCTTGATACC+TGG 0.499591 3_4:+8935458 Msa0488800:five_prime_UTR
GAAGAAGCGGAAGAGGAAGA+AGG 0.503293 3_4:-8934432 None:intergenic
GTAGGAGGGTGTTTGCCACA+TGG 0.503578 3_4:-8933956 None:intergenic
TCTGGAAGAAGAAGCGGAAG+AGG 0.510553 3_4:-8934439 None:intergenic
CACTAGCCCATGGAGGTGCA+GGG 0.517217 3_4:-8936059 None:intergenic
CAACATGAAAATTACAATCC+AGG 0.524034 3_4:-8935476 None:intergenic
GAATGGTTGGAAAGAGCTGT+GGG 0.527955 3_4:-8934022 None:intergenic
GGAAAGAGCTGTGGGAGTGT+GGG 0.530718 3_4:-8934014 None:intergenic
GGAAGAGGAAGAAGGAGCAC+TGG 0.533608 3_4:-8934424 None:intergenic
ATTATTTGCTTAGAAACTCA+AGG 0.536924 3_4:-8936665 None:intergenic
TGGATACCTGAACCTTTGCT+TGG 0.546060 3_4:-8937048 None:intergenic
GGAAGAAGGAGCACTGGTGG+TGG 0.552715 3_4:-8934418 None:intergenic
GGAAGCTCTTCGATTAAACT+CGG 0.557304 3_4:-8934496 None:intergenic
TGTAGTGGCGAAAAGGACTT+CGG 0.570029 3_4:+8934659 Msa0488800:intron
TGTAGTGGCGAAAAGGACTT+CGG 0.570029 3_4:+8935597 Msa0488800:intron
ATTTGATGATCTTCATCATG+TGG 0.570148 3_4:-8937068 None:intergenic
GAAACTATCAAATGTTTCCA+GGG 0.576129 3_4:+8936631 Msa0488800:three_prime_UTR
CACTACTAGTCATTCAACTA+CGG 0.579817 3_4:+8935756 Msa0488800:CDS
ATAAATTACCCACTAGCCCA+TGG 0.581883 3_4:-8936069 None:intergenic
TCATCTGGATCAGCTCCAAA+CGG 0.588128 3_4:+8934393 Msa0488800:five_prime_UTR
TAGGTGCTATTAGCTGGTGT+TGG 0.589758 3_4:+8936586 Msa0488800:three_prime_UTR
AACCTAGTCGTATCTCTCGT+CGG 0.594968 3_4:-8936550 None:intergenic
TTTCTTCAATCTCTCTGCAA+CGG 0.595365 3_4:-8934364 None:intergenic
AACGGACGTTGACGTATGAA+AGG 0.600033 3_4:-8936003 None:intergenic
GTCGGATCTGGAAGAAGAAG+CGG 0.602586 3_4:-8934445 None:intergenic
TTGGGTAACGATTACGAAGG+AGG 0.607110 3_4:-8934198 None:intergenic
GCAAGATTATTCGTCAGAGA+AGG 0.608255 3_4:+8935387 Msa0488800:five_prime_UTR
AACGGCAGATCGAGGATCGT+CGG 0.611645 3_4:-8934463 None:intergenic
TACAATATAATACCAAGCAA+AGG 0.612865 3_4:+8937036 Msa0488800:three_prime_UTR
AAACAATACCTCAATTCGCA+TGG 0.613238 3_4:-8935839 None:intergenic
AGTAATTCTAGAAATGAGAG+TGG 0.617720 3_4:+8935718 Msa0488800:CDS
TGAATTGCAACGGCAGATCG+AGG 0.619223 3_4:-8934471 None:intergenic
GCAGCTGTCTTTGTAGCTAA+CGG 0.619481 3_4:-8936021 None:intergenic
AAAATGACATGTGGCAATGT+GGG 0.621462 3_4:+8933990 Msa0488800:five_prime_UTR
GGAGGGTGTTTGCCACATGG+GGG 0.625821 3_4:-8933953 None:intergenic
ACTAGCCCATGGAGGTGCAG+GGG 0.628852 3_4:-8936058 None:intergenic
AGAGGAAGAAGGAGCACTGG+TGG 0.632090 3_4:-8934421 None:intergenic
AAACTCGGATTGAATTGCAA+CGG 0.637148 3_4:-8934481 None:intergenic
AGTTGAATGACTAGTAGTGT+CGG 0.639278 3_4:-8935752 None:intergenic
ACGATTGATGAGCACTACCA+AGG 0.656559 3_4:+8935798 Msa0488800:CDS
GAGCTTGGAAAGTTTCTGAG+AGG 0.658980 3_4:-8934544 None:intergenic
ATCCGACGAGAGATACGACT+AGG 0.699549 3_4:+8936548 Msa0488800:three_prime_UTR
CACAACAGCAGAGTTAAGAG+CGG 0.716379 3_4:-8935680 None:intergenic
TGTTTGGGTAACGATTACGA+AGG 0.744617 3_4:-8934201 None:intergenic
AATTACCCACTAGCCCATGG+AGG 0.761047 3_4:-8936066 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATATTTGAAATTTCATTT+TGG + chr3_4:8935502-8935521 Msa0488800:intron 10.0%
!!! TATATTTGAAATTTCATTTT+GGG + chr3_4:8935503-8935522 Msa0488800:intron 10.0%
!!! AAATTATGAAAACTTTGTTT+GGG - chr3_4:8934219-8934238 None:intergenic 15.0%
!! AAAATTATGAAAACTTTGTT+TGG - chr3_4:8934220-8934239 None:intergenic 15.0%
!! AAAAATTGAATTGAATTGAA+GGG - chr3_4:8934594-8934613 None:intergenic 15.0%
!!! TAATACTTTTTTTTTTGTAG+TGG + chr3_4:8934644-8934663 Msa0488800:intron 15.0%
!!! AATAATGGGATAATAATAAA+CGG - chr3_4:8934730-8934749 None:intergenic 15.0%
!! AACTATTAAATTGGAATAAT+GGG - chr3_4:8934744-8934763 None:intergenic 15.0%
!! AAACTATTAAATTGGAATAA+TGG - chr3_4:8934745-8934764 None:intergenic 15.0%
!!! AAAAAAGTATTAAACATCAA+GGG - chr3_4:8935575-8935594 None:intergenic 15.0%
!!! AAAAAAAGTATTAAACATCA+AGG - chr3_4:8935576-8935595 None:intergenic 15.0%
!!! TTAATACTTTTTTTTTGTAG+TGG + chr3_4:8935582-8935601 Msa0488800:intron 15.0%
!!! TTATAAGGTTTTAAATAAAG+AGG + chr3_4:8936403-8936422 Msa0488800:intron 15.0%
!!! TATAAGGTTTTAAATAAAGA+GGG + chr3_4:8936404-8936423 Msa0488800:intron 15.0%
!!! AAGATTTTGATTTATTATTG+TGG + chr3_4:8936445-8936464 Msa0488800:intron 15.0%
!! TTGATATAAAAGACTAATAA+TGG + chr3_4:8936485-8936504 Msa0488800:intron 15.0%
!!! ATTTGTTTTAGTTCTATAAA+TGG + chr3_4:8936793-8936812 Msa0488800:intron 15.0%
!! TCTAATTAGAATTGGAATTT+GGG - chr3_4:8934265-8934284 None:intergenic 20.0%
!! ATCTAATTAGAATTGGAATT+TGG - chr3_4:8934266-8934285 None:intergenic 20.0%
!! AATGAAATTGAGATTACATT+TGG - chr3_4:8934571-8934590 None:intergenic 20.0%
!! GAAAAATTGAATTGAATTGA+AGG - chr3_4:8934595-8934614 None:intergenic 20.0%
!! TTTGGGAAAAACTATTAAAT+TGG - chr3_4:8934753-8934772 None:intergenic 20.0%
!!! TTTTTCCCAAATTATTCATA+TGG + chr3_4:8934762-8934781 Msa0488800:intron 20.0%
!! CAATACCATATGAATAATTT+GGG - chr3_4:8934770-8934789 None:intergenic 20.0%
!! ACAATACCATATGAATAATT+TGG - chr3_4:8934771-8934790 None:intergenic 20.0%
!!! ATCAAGTTTATTTTCTTTAG+TGG + chr3_4:8935131-8935150 Msa0488800:intron 20.0%
!!! TCAAGTTTATTTTCTTTAGT+GGG + chr3_4:8935132-8935151 Msa0488800:intron 20.0%
!! AAGGTTAAACTACAAAATAA+AGG - chr3_4:8935263-8935282 None:intergenic 20.0%
!! ATGCTTGATTAAATTATCAA+GGG + chr3_4:8936191-8936210 Msa0488800:intron 20.0%
!! TAAAATTAACATCAGTACAA+TGG - chr3_4:8936356-8936375 None:intergenic 20.0%
!!! TGTTTTTGTTTGAAGTTATA+AGG + chr3_4:8936388-8936407 Msa0488800:intron 20.0%
!!! TTCATGTTTCTTCAATTTTA+TGG + chr3_4:8936825-8936844 Msa0488800:intron 20.0%
! ATGCTCAAAAACAAGAAATA+CGG + chr3_4:8934094-8934113 Msa0488800:intron 25.0%
! TTCCAATTCTAATTAGATCT+AGG + chr3_4:8934268-8934287 Msa0488800:five_prime_UTR 25.0%
! TCCAATTCTAATTAGATCTA+GGG + chr3_4:8934269-8934288 Msa0488800:five_prime_UTR 25.0%
! GATAATAATAAACGGCAATT+TGG - chr3_4:8934722-8934741 None:intergenic 25.0%
! TGTTGCAACTTAAACAATAT+GGG - chr3_4:8934842-8934861 None:intergenic 25.0%
! ATGTTGCAACTTAAACAATA+TGG - chr3_4:8934843-8934862 None:intergenic 25.0%
! CTTTATGATTCAATGTTCAT+GGG + chr3_4:8935032-8935051 Msa0488800:intron 25.0%
!! AATGTGAGTTTCCAATTTTT+GGG - chr3_4:8935104-8935123 None:intergenic 25.0%
!! TAATGTGAGTTTCCAATTTT+TGG - chr3_4:8935105-8935124 None:intergenic 25.0%
! AACATCACCAAAATATATCA+TGG - chr3_4:8935295-8935314 None:intergenic 25.0%
! ATATCAAGTTATTGTGTACT+TGG + chr3_4:8935444-8935463 Msa0488800:five_prime_UTR 25.0%
!! GAATAGAAGACAATTGTTTT+CGG + chr3_4:8935955-8935974 Msa0488800:CDS 25.0%
!! TCTATTGATTGTATTGTTAG+AGG + chr3_4:8936119-8936138 Msa0488800:intron 25.0%
! CATGCTTGATTAAATTATCA+AGG + chr3_4:8936190-8936209 Msa0488800:intron 25.0%
!! ATAAACAATCTCTTGTTTCT+TGG - chr3_4:8936230-8936249 None:intergenic 25.0%
! AAAGAGAACAAATAGACAAA+AGG - chr3_4:8936283-8936302 None:intergenic 25.0%
!! TTTGTCTATTTGTTCTCTTT+TGG + chr3_4:8936283-8936302 Msa0488800:intron 25.0%
! ACTAATAATGGTATATAAGC+TGG + chr3_4:8936497-8936516 Msa0488800:intron 25.0%
! TAGGTTTATGCATACAAATT+AGG + chr3_4:8936567-8936586 Msa0488800:three_prime_UTR 25.0%
! ATTATTTGCTTAGAAACTCA+AGG - chr3_4:8936668-8936687 None:intergenic 25.0%
! TTGAGTTTCTAAGCAAATAA+TGG + chr3_4:8936667-8936686 Msa0488800:three_prime_UTR 25.0%
! TTGCTATGTACATAACTAAA+TGG + chr3_4:8936742-8936761 Msa0488800:intron 25.0%
!! CTTTAGCATAATTGTTTTCA+AGG - chr3_4:8936768-8936787 None:intergenic 25.0%
!! ATGGATAACAACACATATTT+TGG + chr3_4:8936844-8936863 Msa0488800:intron 25.0%
!! TGTAAACCTTTTTATGTCTA+TGG + chr3_4:8936930-8936949 Msa0488800:intron 25.0%
! TCAAATCCATAGACATAAAA+AGG - chr3_4:8936939-8936958 None:intergenic 25.0%
! TACAATATAATACCAAGCAA+AGG + chr3_4:8937036-8937055 Msa0488800:three_prime_UTR 25.0%
!!! TCATTTTTTTATGTGGAGGT+AGG - chr3_4:8933977-8933996 None:intergenic 30.0%
!!! CATGTCATTTTTTTATGTGG+AGG - chr3_4:8933981-8934000 None:intergenic 30.0%
!!! CCACATGTCATTTTTTTATG+TGG - chr3_4:8933984-8934003 None:intergenic 30.0%
CCACATAAAAAAATGACATG+TGG + chr3_4:8933981-8934000 Msa0488800:five_prime_UTR 30.0%
TTGAGCATTCATGATTGTTT+GGG - chr3_4:8934082-8934101 None:intergenic 30.0%
TTTGAGCATTCATGATTGTT+TGG - chr3_4:8934083-8934102 None:intergenic 30.0%
TAACAAACAATCATGAAAGC+TGG + chr3_4:8934145-8934164 Msa0488800:five_prime_UTR 30.0%
! ACGAATTTAGGATTTGGAAA+TGG - chr3_4:8934176-8934195 None:intergenic 30.0%
ACCCTAGATCTAATTAGAAT+TGG - chr3_4:8934273-8934292 None:intergenic 30.0%
TGATTTGAACTACATCTTTG+AGG - chr3_4:8934304-8934323 None:intergenic 30.0%
TTGAAGAAATCAGTTTCATC+TGG + chr3_4:8934378-8934397 Msa0488800:five_prime_UTR 30.0%
!!! TTTTTTTTGTAGTGGCGAAA+AGG + chr3_4:8934652-8934671 Msa0488800:intron 30.0%
! GGCAATTTGGAATGAATAAA+AGG - chr3_4:8934709-8934728 None:intergenic 30.0%
CGTTTGAAAGAAACTTATTG+TGG - chr3_4:8934971-8934990 None:intergenic 30.0%
!! ATCATTGTTGTTTGAAGTTG+AGG - chr3_4:8934994-8935013 None:intergenic 30.0%
TTGAATCATAAAGCAGCAAA+TGG - chr3_4:8935025-8935044 None:intergenic 30.0%
GCTTTATGATTCAATGTTCA+TGG + chr3_4:8935031-8935050 Msa0488800:intron 30.0%
! ATCATCAACAACGTACTTTT+GGG - chr3_4:8935058-8935077 None:intergenic 30.0%
! TATCATCAACAACGTACTTT+TGG - chr3_4:8935059-8935078 None:intergenic 30.0%
!! TTGTTGATGATATGTTGGTT+TGG + chr3_4:8935067-8935086 Msa0488800:intron 30.0%
! GTGATAGCCATGATATATTT+TGG + chr3_4:8935285-8935304 Msa0488800:intron 30.0%
AGTTAAGATACATATCAGCA+TGG - chr3_4:8935350-8935369 None:intergenic 30.0%
CTGTACAAAAAGAATGATGA+CGG + chr3_4:8935410-8935429 Msa0488800:five_prime_UTR 30.0%
CAACATGAAAATTACAATCC+AGG - chr3_4:8935479-8935498 None:intergenic 30.0%
!!! TTTTTTTTGTAGTGGCGAAA+AGG + chr3_4:8934652-8934671 Msa0488800:intron 30.0%
AATTCTTGAATCTCCATTGT+TGG - chr3_4:8935634-8935653 None:intergenic 30.0%
! AGTAATTCTAGAAATGAGAG+TGG + chr3_4:8935718-8935737 Msa0488800:CDS 30.0%
AAGAACATCTTTGAATTCCT+TGG - chr3_4:8935818-8935837 None:intergenic 30.0%
AGAAACTATCAAATGTTTCC+AGG + chr3_4:8936630-8936649 Msa0488800:three_prime_UTR 30.0%
GAAACTATCAAATGTTTCCA+GGG + chr3_4:8936631-8936650 Msa0488800:three_prime_UTR 30.0%
TGACAGACTAACATAAAGAA+TGG - chr3_4:8936714-8936733 None:intergenic 30.0%
!! TAACAACACATATTTTGGAC+TGG + chr3_4:8936849-8936868 Msa0488800:intron 30.0%
!! AACAACACATATTTTGGACT+GGG + chr3_4:8936850-8936869 Msa0488800:intron 30.0%
TTCAAATTGCTATCTTCACA+TGG - chr3_4:8936882-8936901 None:intergenic 30.0%
GAACTACAATCTCATACAAA+GGG - chr3_4:8936906-8936925 None:intergenic 30.0%
TGAACTACAATCTCATACAA+AGG - chr3_4:8936907-8936926 None:intergenic 30.0%
!!! TTTTTTATGTGGAGGTAGGA+GGG - chr3_4:8933973-8933992 None:intergenic 35.0%
!!! TTTTTTTATGTGGAGGTAGG+AGG - chr3_4:8933974-8933993 None:intergenic 35.0%
AAAAATGACATGTGGCAATG+TGG + chr3_4:8933989-8934008 Msa0488800:five_prime_UTR 35.0%
AAAATGACATGTGGCAATGT+GGG + chr3_4:8933990-8934009 Msa0488800:five_prime_UTR 35.0%
! GAGGTAACGAATTTAGGATT+TGG - chr3_4:8934182-8934201 None:intergenic 35.0%
! AATTTGGGATCGATTGATTG+AGG - chr3_4:8934250-8934269 None:intergenic 35.0%
TTTCTTCAATCTCTCTGCAA+CGG - chr3_4:8934367-8934386 None:intergenic 35.0%
AAACTCGGATTGAATTGCAA+CGG - chr3_4:8934484-8934503 None:intergenic 35.0%
AATCGAAGAGCTTCCAAAAT+CGG + chr3_4:8934504-8934523 Msa0488800:five_prime_UTR 35.0%
!! GTGAATTCCATAACCGATTT+TGG - chr3_4:8934520-8934539 None:intergenic 35.0%
! TTATTGGCTATATCATCGTC+AGG + chr3_4:8934926-8934945 Msa0488800:intron 35.0%
!! AAACTTATTGTGGCTTGGTT+AGG - chr3_4:8934961-8934980 None:intergenic 35.0%
GAAAGAAACTTATTGTGGCT+TGG - chr3_4:8934966-8934985 None:intergenic 35.0%
!! GTACGTTGTTGATGATATGT+TGG + chr3_4:8935062-8935081 Msa0488800:intron 35.0%
TATATCATGGCTATCACACA+AGG - chr3_4:8935282-8935301 None:intergenic 35.0%
TAACTCATTATCTACTTCCC+AGG + chr3_4:8935365-8935384 Msa0488800:intron 35.0%
AAACATCAAGGGATGAGTTA+CGG - chr3_4:8935564-8935583 None:intergenic 35.0%
!! GGTTTTTGATGATCCAACAA+TGG + chr3_4:8935618-8935637 Msa0488800:exon 35.0%
AGTTGAATGACTAGTAGTGT+CGG - chr3_4:8935755-8935774 None:intergenic 35.0%
CACTACTAGTCATTCAACTA+CGG + chr3_4:8935756-8935775 Msa0488800:CDS 35.0%
AAACAATACCTCAATTCGCA+TGG - chr3_4:8935842-8935861 None:intergenic 35.0%
! GAGGCTTTAGTTAAGCATAT+AGG + chr3_4:8936138-8936157 Msa0488800:intron 35.0%
! TGCAGTATGACTCAATTTTC+AGG + chr3_4:8936313-8936332 Msa0488800:intron 35.0%
ATAATGGTATATAAGCTGGC+AGG + chr3_4:8936501-8936520 Msa0488800:intron 35.0%
ACAAAAAGAGAGTCATGTGT+AGG - chr3_4:8936527-8936546 None:intergenic 35.0%
!! ACAAATTAGGTGCTATTAGC+TGG + chr3_4:8936580-8936599 Msa0488800:three_prime_UTR 35.0%
TCTACAATATTGTCGTAGCT+TGG + chr3_4:8937007-8937026 Msa0488800:intron 35.0%
CTACAATATTGTCGTAGCTT+GGG + chr3_4:8937008-8937027 Msa0488800:intron 35.0%
ATAATACCAAGCAAAGGTTC+AGG + chr3_4:8937042-8937061 Msa0488800:three_prime_UTR 35.0%
ACGAAGGAGGTAACGAATTT+AGG - chr3_4:8934188-8934207 None:intergenic 40.0%
TGTTTGGGTAACGATTACGA+AGG - chr3_4:8934204-8934223 None:intergenic 40.0%
GGAAGCTCTTCGATTAAACT+CGG - chr3_4:8934499-8934518 None:intergenic 40.0%
AGAGCTTCCAAAATCGGTTA+TGG + chr3_4:8934510-8934529 Msa0488800:five_prime_UTR 40.0%
! TGGAAAGTTTCTGAGAGGTT+TGG - chr3_4:8934542-8934561 None:intergenic 40.0%
TCTGACGAATAATCTTGCCT+GGG - chr3_4:8935385-8935404 None:intergenic 40.0%
GCAAGATTATTCGTCAGAGA+AGG + chr3_4:8935387-8935406 Msa0488800:five_prime_UTR 40.0%
TGCTCATCAATCGTGTCTTT+AGG - chr3_4:8935792-8935811 None:intergenic 40.0%
TGTTCTTACCATGCGAATTG+AGG + chr3_4:8935831-8935850 Msa0488800:CDS 40.0%
ATAAATTACCCACTAGCCCA+TGG - chr3_4:8936072-8936091 None:intergenic 40.0%
GAATGGTTGGAAAGAGCTGT+GGG - chr3_4:8934025-8934044 None:intergenic 45.0%
AGAATGGTTGGAAAGAGCTG+TGG - chr3_4:8934026-8934045 None:intergenic 45.0%
TAGAGAGCAGAGAGAGAGAA+TGG - chr3_4:8934042-8934061 None:intergenic 45.0%
TTGGGTAACGATTACGAAGG+AGG - chr3_4:8934201-8934220 None:intergenic 45.0%
TCATCTGGATCAGCTCCAAA+CGG + chr3_4:8934393-8934412 Msa0488800:five_prime_UTR 45.0%
GAGCTTGGAAAGTTTCTGAG+AGG - chr3_4:8934547-8934566 None:intergenic 45.0%
GAGATTACATTTGGCGAGCT+TGG - chr3_4:8934562-8934581 None:intergenic 45.0%
TGTAGTGGCGAAAAGGACTT+CGG + chr3_4:8934659-8934678 Msa0488800:intron 45.0%
!! TATGTTGGTTTGGCTCTTCC+CGG + chr3_4:8935077-8935096 Msa0488800:intron 45.0%
!! AGTTTCCAATTTTTGGGCCC+GGG - chr3_4:8935098-8935117 None:intergenic 45.0%
!! GAGTTTCCAATTTTTGGGCC+CGG - chr3_4:8935099-8935118 None:intergenic 45.0%
CTCTGACGAATAATCTTGCC+TGG - chr3_4:8935386-8935405 None:intergenic 45.0%
TGTACTTGGAGCTTGATACC+TGG + chr3_4:8935458-8935477 Msa0488800:five_prime_UTR 45.0%
AAGGGATGAGTTACGGCTAA+AGG - chr3_4:8935557-8935576 None:intergenic 45.0%
TGTAGTGGCGAAAAGGACTT+CGG + chr3_4:8934659-8934678 Msa0488800:intron 45.0%
CACAACAGCAGAGTTAAGAG+CGG - chr3_4:8935683-8935702 None:intergenic 45.0%
ACGATTGATGAGCACTACCA+AGG + chr3_4:8935798-8935817 Msa0488800:CDS 45.0%
AACGGACGTTGACGTATGAA+AGG - chr3_4:8936006-8936025 None:intergenic 45.0%
GCAGCTGTCTTTGTAGCTAA+CGG - chr3_4:8936024-8936043 None:intergenic 45.0%
AACCTAGTCGTATCTCTCGT+CGG - chr3_4:8936553-8936572 None:intergenic 45.0%
!! TAGGTGCTATTAGCTGGTGT+TGG + chr3_4:8936586-8936605 Msa0488800:three_prime_UTR 45.0%
!! TGCTATTAGCTGGTGTTGGT+TGG + chr3_4:8936590-8936609 Msa0488800:three_prime_UTR 45.0%
TCAAGGATCTTTAGATGCCC+TGG - chr3_4:8936651-8936670 None:intergenic 45.0%
TGGATACCTGAACCTTTGCT+TGG - chr3_4:8937051-8937070 None:intergenic 45.0%
!! CAAAATAAAAAAAAAAAATT+TGG - chr3_4:8934903-8934922 None:intergenic 5.0%
!!! TTTTTTTATTTTGTTATTAT+TGG + chr3_4:8934910-8934929 Msa0488800:intron 5.0%
!! TAGGAGGGTGTTTGCCACAT+GGG - chr3_4:8933958-8933977 None:intergenic 50.0%
GAGCAGAGAGAGAGAATGGT+TGG - chr3_4:8934038-8934057 None:intergenic 50.0%
GAAGAAGCGGAAGAGGAAGA+AGG - chr3_4:8934435-8934454 None:intergenic 50.0%
TCTGGAAGAAGAAGCGGAAG+AGG - chr3_4:8934442-8934461 None:intergenic 50.0%
GTCGGATCTGGAAGAAGAAG+CGG - chr3_4:8934448-8934467 None:intergenic 50.0%
TGAATTGCAACGGCAGATCG+AGG - chr3_4:8934474-8934493 None:intergenic 50.0%
!! ATGTTGGTTTGGCTCTTCCC+GGG + chr3_4:8935078-8935097 Msa0488800:intron 50.0%
ATCCTGAAGAGCAGCGTAGA+AGG + chr3_4:8935202-8935221 Msa0488800:intron 50.0%
GGATGAGTTACGGCTAAAGG+AGG - chr3_4:8935554-8935573 None:intergenic 50.0%
CGCTCTTAACTCTGCTGTTG+TGG + chr3_4:8935681-8935700 Msa0488800:CDS 50.0%
AATTACCCACTAGCCCATGG+AGG - chr3_4:8936069-8936088 None:intergenic 50.0%
ATCCGACGAGAGATACGACT+AGG + chr3_4:8936548-8936567 Msa0488800:three_prime_UTR 50.0%
!! CATGGGGGTGTTCACCTATC+AGG - chr3_4:8933941-8933960 None:intergenic 55.0%
GATAGGTGAACACCCCCATG+TGG + chr3_4:8933941-8933960 Msa0488800:five_prime_UTR 55.0%
!! AGGAGGGTGTTTGCCACATG+GGG - chr3_4:8933957-8933976 None:intergenic 55.0%
!! GTAGGAGGGTGTTTGCCACA+TGG - chr3_4:8933959-8933978 None:intergenic 55.0%
! GGAAAGAGCTGTGGGAGTGT+GGG - chr3_4:8934017-8934036 None:intergenic 55.0%
TGGAAAGAGCTGTGGGAGTG+TGG - chr3_4:8934018-8934037 None:intergenic 55.0%
!! AGAGGAAGAAGGAGCACTGG+TGG - chr3_4:8934424-8934443 None:intergenic 55.0%
! GGAAGAGGAAGAAGGAGCAC+TGG - chr3_4:8934427-8934446 None:intergenic 55.0%
AGATCGAGGATCGTCGGATC+TGG - chr3_4:8934460-8934479 None:intergenic 55.0%
AACGGCAGATCGAGGATCGT+CGG - chr3_4:8934466-8934485 None:intergenic 55.0%
CTCTTCCCGGGCCCAAAAAT+TGG + chr3_4:8935090-8935109 Msa0488800:intron 55.0%
GTCCTTCTACGCTGCTCTTC+AGG - chr3_4:8935207-8935226 None:intergenic 55.0%
!! GGAGGGTGTTTGCCACATGG+GGG - chr3_4:8933956-8933975 None:intergenic 60.0%
!! GGAAGAAGGAGCACTGGTGG+TGG - chr3_4:8934421-8934440 None:intergenic 60.0%
TAGTGCCCCTGCACCTCCAT+GGG + chr3_4:8936053-8936072 Msa0488800:three_prime_UTR 60.0%
! ACTAGCCCATGGAGGTGCAG+GGG - chr3_4:8936061-8936080 None:intergenic 60.0%
! CACTAGCCCATGGAGGTGCA+GGG - chr3_4:8936062-8936081 None:intergenic 60.0%
!! CTGCACCTCCATGGGCTAGT+GGG + chr3_4:8936061-8936080 Msa0488800:three_prime_UTR 60.0%
!! GCACTGGTGGTGGCTCCGTT+TGG - chr3_4:8934411-8934430 None:intergenic 65.0%
CTAGTGCCCCTGCACCTCCA+TGG + chr3_4:8936052-8936071 Msa0488800:three_prime_UTR 65.0%
! CCACTAGCCCATGGAGGTGC+AGG - chr3_4:8936063-8936082 None:intergenic 65.0%
!! CCTGCACCTCCATGGGCTAG+TGG + chr3_4:8936060-8936079 Msa0488800:three_prime_UTR 65.0%
Chromosome Type Strat End Strand Name
chr3_4 gene 8933934 8937088 8933934 ID=Msa0488800;Name=Msa0488800
chr3_4 mRNA 8933934 8937088 8933934 ID=Msa0488800-mRNA-1;Parent=Msa0488800;Name=Msa0488800-mRNA-1;_AED=0.31;_eAED=0.31;_QI=730|1|1|1|0.14|0.12|8|285|101
chr3_4 exon 8933934 8934054 8933934 ID=Msa0488800-mRNA-1:exon:2883;Parent=Msa0488800-mRNA-1
chr3_4 exon 8934134 8934573 8934134 ID=Msa0488800-mRNA-1:exon:2884;Parent=Msa0488800-mRNA-1
chr3_4 exon 8934664 8934700 8934664 ID=Msa0488800-mRNA-1:exon:2885;Parent=Msa0488800-mRNA-1
chr3_4 exon 8935387 8935483 8935387 ID=Msa0488800-mRNA-1:exon:2886;Parent=Msa0488800-mRNA-1
chr3_4 exon 8935602 8935852 8935602 ID=Msa0488800-mRNA-1:exon:2887;Parent=Msa0488800-mRNA-1
chr3_4 exon 8935938 8936082 8935938 ID=Msa0488800-mRNA-1:exon:2888;Parent=Msa0488800-mRNA-1
chr3_4 exon 8936523 8936688 8936523 ID=Msa0488800-mRNA-1:exon:2889;Parent=Msa0488800-mRNA-1
chr3_4 exon 8937025 8937088 8937025 ID=Msa0488800-mRNA-1:exon:2890;Parent=Msa0488800-mRNA-1
chr3_4 five_prime_UTR 8933934 8934054 8933934 ID=Msa0488800-mRNA-1:five_prime_utr;Parent=Msa0488800-mRNA-1
chr3_4 five_prime_UTR 8934134 8934573 8934134 ID=Msa0488800-mRNA-1:five_prime_utr;Parent=Msa0488800-mRNA-1
chr3_4 five_prime_UTR 8934664 8934700 8934664 ID=Msa0488800-mRNA-1:five_prime_utr;Parent=Msa0488800-mRNA-1
chr3_4 five_prime_UTR 8935387 8935483 8935387 ID=Msa0488800-mRNA-1:five_prime_utr;Parent=Msa0488800-mRNA-1
chr3_4 five_prime_UTR 8935602 8935636 8935602 ID=Msa0488800-mRNA-1:five_prime_utr;Parent=Msa0488800-mRNA-1
chr3_4 CDS 8935637 8935852 8935637 ID=Msa0488800-mRNA-1:cds;Parent=Msa0488800-mRNA-1
chr3_4 CDS 8935938 8936027 8935938 ID=Msa0488800-mRNA-1:cds;Parent=Msa0488800-mRNA-1
chr3_4 three_prime_UTR 8936028 8936082 8936028 ID=Msa0488800-mRNA-1:three_prime_utr;Parent=Msa0488800-mRNA-1
chr3_4 three_prime_UTR 8936523 8936688 8936523 ID=Msa0488800-mRNA-1:three_prime_utr;Parent=Msa0488800-mRNA-1
chr3_4 three_prime_UTR 8937025 8937088 8937025 ID=Msa0488800-mRNA-1:three_prime_utr;Parent=Msa0488800-mRNA-1
Gene Sequence

>Msa0488800

ATGGAGATTCAAGAATTAACTAGTGTTATCAAGCAAGATATTACCGCTCTTAACTCTGCTGTTGTGGATCTTCAATTAATCAGTAATTCTAGAAATGAGAGTGGAAATGTTTCTACCGACACTACTAGTCATTCAACTACGGTTGTCGATGACCTAAAGACACGATTGATGAGCACTACCAAGGAATTCAAAGATGTTCTTACCATGCGAATTGAGAATTTGAAAGTGCATGAGAATAGAAGACAATTGTTTTCGGCGAATGCTTCTAAGATTCTGCAAATCCTTTCATACGTCAACGTCCGTTAG
Protein sequence

>Msa0488800

MEIQELTSVIKQDITALNSAVVDLQLISNSRNESGNVSTDTTSHSTTVVDDLKTRLMSTTKEFKDVLTMRIENLKVHENRRQLFSANASKILQILSYVNVR*