AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1383330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1383330 MtrunA17_Chr3g0135831 96.063 381 12 1 1 381 1 378 0.0 753
Msa1383330 MtrunA17_Chr7g0260331 54.924 264 112 2 8 264 8 271 6.12e-94 285
Msa1383330 MtrunA17_Chr1g0204741 62.963 216 73 4 36 246 27 240 9.73e-92 278
Msa1383330 MtrunA17_Chr5g0410881 55.357 224 84 4 36 247 21 240 1.99e-80 255
Msa1383330 MtrunA17_Chr4g0071051 40.431 371 177 11 24 376 76 420 6.44e-77 243
Msa1383330 MtrunA17_Chr4g0039891 54.808 208 86 2 48 247 11 218 3.23e-73 236
Msa1383330 MtrunA17_Chr1g0185131 44.224 303 143 8 36 331 19 302 3.68e-73 231
Msa1383330 MtrunA17_Chr6g0485571 45.274 201 106 2 49 249 11 207 6.18e-53 179
Msa1383330 MtrunA17_Chr8g0379701 40.517 232 130 2 48 275 11 238 4.90e-49 170
Msa1383330 MtrunA17_Chr8g0392001 45.876 194 98 2 49 235 22 215 5.43e-49 169
Msa1383330 MtrunA17_Chr8g0378061 43.284 201 104 5 50 247 23 216 5.54e-46 164
Msa1383330 MtrunA17_Chr2g0330821 40.933 193 110 1 43 235 73 261 5.71e-46 161
Msa1383330 MtrunA17_Chr6g0467041 42.365 203 107 5 49 248 22 217 6.72e-46 160
Msa1383330 MtrunA17_Chr2g0284481 41.243 177 101 1 48 221 14 190 2.00e-43 155
Msa1383330 MtrunA17_Chr5g0397881 62.766 94 35 0 47 140 22 115 5.56e-38 140
Msa1383330 MtrunA17_Chr7g0257331 45.062 162 76 3 40 188 9 170 1.76e-37 135
Msa1383330 MtrunA17_Chr3g0134031 56.757 111 43 1 43 153 18 123 8.26e-37 137
Msa1383330 MtrunA17_Chr5g0402291 55.882 102 45 0 46 147 6 107 8.93e-37 135
Msa1383330 MtrunA17_Chr6g0472421 58.511 94 39 0 47 140 31 124 9.08e-35 130
Msa1383330 MtrunA17_Chr1g0203601 54.206 107 49 0 47 153 16 122 1.28e-34 129
Msa1383330 MtrunA17_Chr1g0179311 38.537 205 120 4 109 310 14 215 4.45e-34 129
Msa1383330 MtrunA17_Chr8g0375041 52.727 110 50 1 44 153 4 111 5.25e-34 126
Msa1383330 MtrunA17_Chr5g0441531 60.638 94 37 0 47 140 23 116 6.83e-34 126
Msa1383330 MtrunA17_Chr4g0047421 36.957 184 101 5 28 204 17 192 4.78e-29 112
Msa1383330 MtrunA17_Chr1g0179321 63.514 74 27 0 35 108 35 108 3.02e-26 101
Msa1383330 MtrunA17_Chr4g0023771 51.429 70 33 1 40 108 6 75 6.52e-18 79.0
Msa1383330 MtrunA17_Chr2g0330841 32.051 156 97 4 96 247 26 176 1.71e-16 80.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1383330 AT2G26150.1 51.862 349 124 7 36 379 31 340 2.57e-121 355
Msa1383330 AT2G26150.4 51.862 349 124 7 36 379 31 340 2.57e-121 355
Msa1383330 AT3G22830.1 44.258 357 158 8 36 359 48 396 5.94e-89 274
Msa1383330 AT3G51910.1 55.357 224 79 3 39 256 19 227 7.04e-77 239
Msa1383330 AT4G17750.1 51.570 223 91 2 47 252 50 272 3.11e-76 244
Msa1383330 AT1G32330.1 44.816 299 146 7 15 298 3 297 2.56e-75 241
Msa1383330 AT1G32330.2 44.816 299 146 7 15 298 3 297 3.19e-75 241
Msa1383330 AT5G16820.1 50.655 229 97 3 27 244 10 233 1.37e-74 239
Msa1383330 AT5G16820.2 50.655 229 97 3 27 244 10 233 1.37e-74 239
Msa1383330 AT3G02990.1 53.589 209 86 3 48 247 22 228 2.05e-73 236
Msa1383330 AT3G63350.1 43.492 315 118 7 25 331 4 266 1.80e-70 223
Msa1383330 AT5G43840.1 47.863 234 105 3 36 269 6 222 2.60e-69 219
Msa1383330 AT2G26150.3 44.404 277 108 8 109 379 49 285 5.76e-68 216
Msa1383330 AT2G26150.2 44.404 277 108 8 109 379 49 285 5.76e-68 216
Msa1383330 AT5G03720.2 45.815 227 111 4 17 240 24 241 1.09e-60 201
Msa1383330 AT5G03720.1 45.815 227 111 4 17 240 24 241 1.09e-60 201
Msa1383330 AT1G67970.1 47.872 188 97 1 49 235 19 206 5.74e-56 188
Msa1383330 AT5G54070.1 42.857 217 109 3 42 257 63 265 1.45e-55 186
Msa1383330 AT4G13980.1 40.201 199 114 2 50 247 24 218 3.98e-48 170
Msa1383330 AT3G24520.1 45.411 207 86 5 49 251 17 200 1.56e-47 165
Msa1383330 AT5G45710.3 38.725 204 118 2 48 251 12 208 2.92e-37 138
Msa1383330 AT5G45710.1 38.725 204 118 2 48 251 12 208 2.92e-37 138
Msa1383330 AT4G36990.1 52.632 114 48 1 47 160 12 119 1.53e-36 134
Msa1383330 AT1G46264.1 38.191 199 70 3 39 187 24 219 2.83e-36 135
Msa1383330 AT2G41690.1 48.889 135 52 2 6 140 14 131 5.48e-36 132
Msa1383330 AT4G18880.1 44.000 175 97 1 48 221 14 188 1.19e-35 135
Msa1383330 AT5G62020.1 55.882 102 45 0 47 148 21 122 1.79e-35 132
Msa1383330 AT4G11660.1 58.511 94 39 0 47 140 57 150 2.10e-34 131
Msa1383330 AT4G18870.1 34.711 121 75 1 49 165 146 266 1.31e-20 91.3
Msa1383330 AT4G18870.1 43.678 87 48 1 50 136 14 99 6.88e-18 83.6
Msa1383330 AT1G77570.1 36.667 90 54 2 50 136 27 116 6.34e-13 66.2

Find 183 sgRNAs with CRISPR-Local

Find 230 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATGCTTTATCCATAAATT+TGG 0.091146 tig0020594:-18852 Msa1383330:three_prime_UTR
GAATCAACGCCCCAAATTTA+TGG 0.128034 tig0020594:+18841 None:intergenic
TTATCCTGGTCAAAGAAATT+TGG 0.160792 tig0020594:+19679 None:intergenic
TTGGATTAAAATATATATTA+AGG 0.180387 tig0020594:-19482 Msa1383330:three_prime_UTR
GGTGGTGCTTGTATTGAATT+AGG 0.198844 tig0020594:-20941 Msa1383330:CDS
CAGTTTGCTTTCTTTGTTGC+TGG 0.209711 tig0020594:-19573 Msa1383330:three_prime_UTR
TGTGATGTGATAATCGATTT+TGG 0.223359 tig0020594:+21740 None:intergenic
TGTTGTTTGGTTCAAAATAA+AGG 0.233150 tig0020594:+20036 None:intergenic
GAGAATTTGCAAGATGATTC+TGG 0.234265 tig0020594:-20638 Msa1383330:CDS
CTCGTAATAGCTTTGTTGTT+TGG 0.240497 tig0020594:-21466 Msa1383330:CDS
TGGTAGAAAATAATGATAAT+TGG 0.250597 tig0020594:-20322 Msa1383330:CDS
AAATGGAGCAACTTAATTTA+AGG 0.257017 tig0020594:+21859 None:intergenic
AAGTGGTAATGAGAGTAATT+TGG 0.259305 tig0020594:-20453 Msa1383330:CDS
TTCAACAACATCAAAGGTTT+TGG 0.271463 tig0020594:+21526 None:intergenic
AATGCTTTATCCATAAATTT+GGG 0.272774 tig0020594:-18851 Msa1383330:three_prime_UTR
TCAGCCGATTCTGTTCCTTT+TGG 0.275927 tig0020594:-19763 Msa1383330:three_prime_UTR
GAGACGGGTGAAGAGAAATT+TGG 0.277699 tig0020594:+21907 None:intergenic
TGCAATCCTTCCATTGGTTT+TGG 0.278910 tig0020594:+21575 None:intergenic
CCACTGATTCCATTGTTTCA+TGG 0.288131 tig0020594:-21496 Msa1383330:CDS
TTGAGTGAATATTAGTGATT+TGG 0.288171 tig0020594:-19293 Msa1383330:three_prime_UTR
TCGTAATAGCTTTGTTGTTT+GGG 0.292309 tig0020594:-21465 Msa1383330:CDS
TCAATGCTTACAATTGAATA+TGG 0.303918 tig0020594:-19395 Msa1383330:three_prime_UTR
CTTGTGTTGGTTCGAATCTC+TGG 0.307706 tig0020594:+18740 None:intergenic
AACTCTTCATCTTCCTCTTC+GGG 0.307939 tig0020594:-21611 Msa1383330:CDS
TCAATCATCTTGACAACTTT+GGG 0.309794 tig0020594:+19903 None:intergenic
CCAATCACAACTTCATCTTC+TGG 0.312679 tig0020594:+20380 None:intergenic
TGTATTGAATTAGGGGAGTA+TGG 0.320828 tig0020594:-20932 Msa1383330:CDS
TAACTCTTCATCTTCCTCTT+CGG 0.322245 tig0020594:-21612 Msa1383330:CDS
TCACTTGAGTTGCACCTTGC+TGG 0.323733 tig0020594:-20214 Msa1383330:intron
TATTTAAACACCACACCATC+TGG 0.328747 tig0020594:+18904 None:intergenic
TTGGGATTAGATAGATAGAT+TGG 0.336724 tig0020594:+21827 None:intergenic
TTCAATCATCTTGACAACTT+TGG 0.337501 tig0020594:+19902 None:intergenic
TATGAAAGGTAGTTATGTTA+TGG 0.348109 tig0020594:+21788 None:intergenic
GGATATGGTGTTCATGGAAT+TGG 0.357483 tig0020594:-19526 Msa1383330:three_prime_UTR
CATTGTGAATTGAGTGTTGC+TGG 0.360684 tig0020594:-19245 Msa1383330:three_prime_UTR
GCTCACTGCTAGTTCGAGTT+TGG 0.362423 tig0020594:-20660 Msa1383330:CDS
TAATCTCGCGGAGGAGGATA+TGG 0.369052 tig0020594:-19541 Msa1383330:three_prime_UTR
TTAGCATATGTCAGTGTTGT+TGG 0.370710 tig0020594:-21647 Msa1383330:CDS
TGATGTGCCCGTGGAGGATT+TGG 0.372993 tig0020594:-20342 Msa1383330:CDS
TGTTGGTGGAATGGTGGATT+TGG 0.375232 tig0020594:-19354 Msa1383330:three_prime_UTR
GCAGAGCCTTGTTGATCAAA+TGG 0.381911 tig0020594:-20288 Msa1383330:CDS
TGAATCAAGCAGTGAAGTTA+AGG 0.388539 tig0020594:-20501 Msa1383330:CDS
AGAGAAATTTGGTTTGGTAA+TGG 0.389818 tig0020594:+21918 None:intergenic
CTGATGGAGTGATGGTTGTC+AGG 0.392404 tig0020594:-19141 Msa1383330:three_prime_UTR
GTTCACAACAATATGTTGTT+TGG 0.395706 tig0020594:+20023 None:intergenic
AGTTGGCCTGTATCTTTATA+TGG 0.396126 tig0020594:-19115 Msa1383330:three_prime_UTR
TGGTGTTTAAATAGACATAT+TGG 0.397087 tig0020594:-18894 Msa1383330:three_prime_UTR
AATGGAAGGATTGCACGAAA+TGG 0.400329 tig0020594:-21567 Msa1383330:CDS
AGTGGTAATGAGAGTAATTT+GGG 0.400460 tig0020594:-20452 Msa1383330:CDS
GATGATTGAATGGGAAAAGT+TGG 0.400946 tig0020594:-19889 Msa1383330:three_prime_UTR
CTCCTATTGTTCCTCGTGCT+TGG 0.402787 tig0020594:-19024 Msa1383330:three_prime_UTR
TGGATTAAAATATATATTAA+GGG 0.408953 tig0020594:-19481 Msa1383330:three_prime_UTR
AAGATGATTCTGGAGCTATA+AGG 0.413178 tig0020594:-20628 Msa1383330:CDS
GTGGTGCTTGTATTGAATTA+GGG 0.418246 tig0020594:-20940 Msa1383330:CDS
TGGCTGAGATTGTGAAGTTA+AGG 0.421096 tig0020594:-20862 Msa1383330:CDS
TAGATTGGGGAAGAGTGAAA+TGG 0.424599 tig0020594:+21842 None:intergenic
TTCTACAACCAATTTGACTA+AGG 0.426171 tig0020594:+20257 None:intergenic
TACAAACTATTCTAAAACTA+GGG 0.426336 tig0020594:+18797 None:intergenic
TTACAAACTATTCTAAAACT+AGG 0.427025 tig0020594:+18796 None:intergenic
CAGAGCCTTGTTGATCAAAT+GGG 0.427250 tig0020594:-20287 Msa1383330:CDS
TCTTGAGAGCATATATATGA+AGG 0.444253 tig0020594:-19449 Msa1383330:three_prime_UTR
TGGGATTAGATAGATAGATT+GGG 0.444600 tig0020594:+21828 None:intergenic
AGATGATTCTGGAGCTATAA+GGG 0.444673 tig0020594:-20627 Msa1383330:CDS
GCATATATATGAAGGGTTGA+AGG 0.448803 tig0020594:-19441 Msa1383330:three_prime_UTR
CTCTTGTAAACGGGGCCTGA+TGG 0.452618 tig0020594:-19157 Msa1383330:three_prime_UTR
TTAGGGGAGTATGGTCTTGA+AGG 0.455498 tig0020594:-20923 Msa1383330:CDS
AGCCAAGCACGAGGAACAAT+AGG 0.457798 tig0020594:+19022 None:intergenic
ATGATTGAATGGGAAAAGTT+GGG 0.459488 tig0020594:-19888 Msa1383330:three_prime_UTR
GAAGGGAATTAGAGTGAAAG+AGG 0.460183 tig0020594:-21678 Msa1383330:CDS
GTGAGATTGAGAGGTTGAGA+AGG 0.465030 tig0020594:-20901 Msa1383330:CDS
GATTCCCACCAACCAACTCT+TGG 0.469023 tig0020594:+20402 None:intergenic
GGAGGAGGATATGGTGTTCA+TGG 0.471635 tig0020594:-19532 Msa1383330:three_prime_UTR
CATTGTTCGCGGGTCAGTCT+AGG 0.473031 tig0020594:-19623 Msa1383330:three_prime_UTR
ATGGAAGGATTGCACGAAAT+GGG 0.473051 tig0020594:-21566 Msa1383330:CDS
GGTTCGAATCTCTGGTGTTA+CGG 0.475248 tig0020594:+18748 None:intergenic
TAATCACCATATAAAGATAC+AGG 0.475577 tig0020594:+19109 None:intergenic
CACGAGGAACAATAGGAGTA+TGG 0.476046 tig0020594:+19029 None:intergenic
TGAATGGCTTTGTATGCAGC+AGG 0.477650 tig0020594:-20096 Msa1383330:three_prime_UTR
CAAGCTATGCAGCAAGAGAC+TGG 0.480304 tig0020594:-20962 Msa1383330:CDS
TAATCAACCACAGACTCAAT+TGG 0.480632 tig0020594:+20599 None:intergenic
ATTCGTAATTCGTATCACTT+TGG 0.481209 tig0020594:-18943 Msa1383330:three_prime_UTR
ATTTCGTGCAATCCTTCCAT+TGG 0.481224 tig0020594:+21569 None:intergenic
GGGTGAAGAGAAATTTGGTT+TGG 0.483584 tig0020594:+21912 None:intergenic
CATTAGGATAGAATAAGAAA+TGG 0.486421 tig0020594:+19952 None:intergenic
GCGTTGAAATGAAGCGAAAA+CGG 0.487154 tig0020594:-20685 Msa1383330:CDS
GGAAAGTTGGTAAGTATGAA+AGG 0.490210 tig0020594:+21774 None:intergenic
AGCAACTTAATTTAAGGCGG+TGG 0.494578 tig0020594:+21865 None:intergenic
TGAGATTGAGAGGTTGAGAA+GGG 0.495911 tig0020594:-20900 Msa1383330:CDS
AAGGGAGCAATTATCTGCTA+TGG 0.496135 tig0020594:-20822 Msa1383330:CDS
TGATGGTTGTCAGGTGGAGT+TGG 0.497598 tig0020594:-19132 Msa1383330:three_prime_UTR
ATGATGTGTTGCTAGCAATT+CGG 0.498371 tig0020594:+19838 None:intergenic
TTGTTCCTCGTGCTTGGCTG+TGG 0.498392 tig0020594:-19018 Msa1383330:three_prime_UTR
GGCAGCAGCAGCATAATTCA+AGG 0.500409 tig0020594:-20841 Msa1383330:CDS
CGATGGGAGTTTGCGAACGA+AGG 0.501252 tig0020594:-21049 Msa1383330:CDS
AGCATCAACTAACTCAATGA+AGG 0.501749 tig0020594:-21696 Msa1383330:exon
TACTTGCCATCTTGCCCAGA+TGG 0.503354 tig0020594:-18919 Msa1383330:three_prime_UTR
TTGAGAGGTTGAGAAGGGAC+AGG 0.504469 tig0020594:-20895 Msa1383330:CDS
CTAGCAACACATCATTCGTT+AGG 0.508340 tig0020594:-19830 Msa1383330:three_prime_UTR
GGATCCAAATTTCTTTGACC+AGG 0.509136 tig0020594:-19683 Msa1383330:three_prime_UTR
GTGTTCTGTACATTGTTCGC+GGG 0.509958 tig0020594:-19633 Msa1383330:three_prime_UTR
TTCGCAAACTCCCATCGATC+CGG 0.510214 tig0020594:+21055 None:intergenic
AAGTTGTCAAGATGATTGAA+TGG 0.521031 tig0020594:-19899 Msa1383330:three_prime_UTR
CTGACAACCATCACTCCATC+AGG 0.521916 tig0020594:+19142 None:intergenic
AATTATCTGCTATGGAAGCT+AGG 0.522811 tig0020594:-20814 Msa1383330:CDS
CAGAGATGACAGTTCCCAAA+AGG 0.524119 tig0020594:+19748 None:intergenic
CATATATATGAAGGGTTGAA+GGG 0.524858 tig0020594:-19440 Msa1383330:three_prime_UTR
AGTTGTCAAGATGATTGAAT+GGG 0.528119 tig0020594:-19898 Msa1383330:three_prime_UTR
TTTAAGGCGGTGGATAGAGT+AGG 0.530038 tig0020594:+21875 None:intergenic
CCAGAAGATGAAGTTGTGAT+TGG 0.531597 tig0020594:-20380 Msa1383330:CDS
ACCACACCATCTGGGCAAGA+TGG 0.532508 tig0020594:+18913 None:intergenic
GACTACTATTGAGTCTGAGA+TGG 0.532873 tig0020594:-20549 Msa1383330:CDS
CTTGAGAGCATATATATGAA+GGG 0.534563 tig0020594:-19448 Msa1383330:three_prime_UTR
CTGAACCAAGAGTTGGTTGG+TGG 0.536158 tig0020594:-20407 Msa1383330:CDS
AAAAGTTGATCCGGATCGAT+GGG 0.538609 tig0020594:-21065 Msa1383330:CDS
CACTGGTGATTGTATATACG+TGG 0.538875 tig0020594:-19075 Msa1383330:three_prime_UTR
AGTAGGAAGGTTCTAGAGAC+GGG 0.539491 tig0020594:+21892 None:intergenic
GAAAAGTTGATCCGGATCGA+TGG 0.539674 tig0020594:-21066 Msa1383330:CDS
GATGGGAGTTTGCGAACGAA+GGG 0.539714 tig0020594:-21048 Msa1383330:CDS
AATAAAGGATACACATGTGA+TGG 0.541183 tig0020594:+20051 None:intergenic
GTTCCCAAAAGGAACAGAAT+CGG 0.541652 tig0020594:+19759 None:intergenic
GGATAGAATAAGAAATGGTA+TGG 0.543377 tig0020594:+19957 None:intergenic
GGATTTACTGAACCAAGAGT+TGG 0.543897 tig0020594:-20414 Msa1383330:CDS
CTAAAGATCAGAATTTATCC+TGG 0.545378 tig0020594:+19665 None:intergenic
AGGGTCTCATTGTTATGTGT+TGG 0.546403 tig0020594:-19187 Msa1383330:three_prime_UTR
TTTGGGTGATGCTGTGTGGG+AGG 0.547697 tig0020594:-20435 Msa1383330:CDS
GGGATTAGATAGATAGATTG+GGG 0.551400 tig0020594:+21829 None:intergenic
TGTGTTCTGTACATTGTTCG+CGG 0.551582 tig0020594:-19634 Msa1383330:three_prime_UTR
ATATGGTGATTAACTTCAAG+AGG 0.551845 tig0020594:-19098 Msa1383330:three_prime_UTR
TGATTAACTTCAAGAGGCAC+TGG 0.556625 tig0020594:-19092 Msa1383330:three_prime_UTR
TAATTTGGGTGATGCTGTGT+GGG 0.557732 tig0020594:-20438 Msa1383330:CDS
TGGATCTTCAACAACATCAA+AGG 0.559140 tig0020594:+21520 None:intergenic
GAATTGTAAATCGAATAGCT+TGG 0.564977 tig0020594:-19501 Msa1383330:three_prime_UTR
GAGTAGGAAGGTTCTAGAGA+CGG 0.570181 tig0020594:+21891 None:intergenic
AAATTACTCTCATTACCACT+TGG 0.571022 tig0020594:+20455 None:intergenic
AAATCTTACACCAAAACCAA+TGG 0.575232 tig0020594:-21585 Msa1383330:CDS
GTCTTGAAGGTGAGATTGAG+AGG 0.580331 tig0020594:-20910 Msa1383330:CDS
GCCATCTTGCCCAGATGGTG+TGG 0.581107 tig0020594:-18914 Msa1383330:three_prime_UTR
GGAAGATTGTACTGCAGAAG+AGG 0.582683 tig0020594:-20074 Msa1383330:three_prime_UTR
ATGCTTTATCCATAAATTTG+GGG 0.583932 tig0020594:-18850 Msa1383330:three_prime_UTR
TGTGAATTGAGTGTTGCTGG+TGG 0.588769 tig0020594:-19242 Msa1383330:three_prime_UTR
CTCTTGTCACGAGCAGCAAG+AGG 0.588881 tig0020594:-20576 Msa1383330:CDS
CCATGAAACAATGGAATCAG+TGG 0.596228 tig0020594:+21496 None:intergenic
TATGGATAAAGCATTGTCAA+CGG 0.599610 tig0020594:+18859 None:intergenic
GAAACAATGGAATCAGTGGA+TGG 0.602123 tig0020594:+21500 None:intergenic
ATTTAAACACCACACCATCT+GGG 0.605166 tig0020594:+18905 None:intergenic
CAAAACCCATTTGATCAACA+AGG 0.605749 tig0020594:+20282 None:intergenic
TAATTGATGTAGAATATGAA+TGG 0.607697 tig0020594:-20112 Msa1383330:intron
GCATCAACTAACTCAATGAA+GGG 0.610881 tig0020594:-21695 Msa1383330:exon
CTGGTGATTGTATATACGTG+GGG 0.612769 tig0020594:-19073 Msa1383330:three_prime_UTR
TGGTGCTTGTATTGAATTAG+GGG 0.613155 tig0020594:-20939 Msa1383330:CDS
TGAGAGGTTGAGAAGGGACA+GGG 0.615785 tig0020594:-20894 Msa1383330:CDS
TGAACCAAGAGTTGGTTGGT+GGG 0.615910 tig0020594:-20406 Msa1383330:CDS
GATTCCATTGTTTCATGGAG+CGG 0.618876 tig0020594:-21491 Msa1383330:CDS
AAATAATGATAATTGGACTG+TGG 0.619002 tig0020594:-20315 Msa1383330:CDS
AACCACAGACTCAATTGGCA+CGG 0.620284 tig0020594:+20604 None:intergenic
TGTAAGATTTGAACCCGAAG+AGG 0.623342 tig0020594:+21598 None:intergenic
GCAGCAGCAGCATAATTCAA+GGG 0.624420 tig0020594:-20840 Msa1383330:CDS
GGCCGTGCCAATTGAGTCTG+TGG 0.628717 tig0020594:-20606 Msa1383330:CDS
AACGGGGCCTGATGGAGTGA+TGG 0.628748 tig0020594:-19149 Msa1383330:three_prime_UTR
TTCTCAAATTGATGTGCCCG+TGG 0.628790 tig0020594:-20351 Msa1383330:CDS
CATCAGGCCCCGTTTACAAG+AGG 0.628875 tig0020594:+19158 None:intergenic
ATGGAGTGATGGTTGTCAGG+TGG 0.629208 tig0020594:-19138 Msa1383330:three_prime_UTR
GTAACGAGGTAAAATAGCAG+TGG 0.630092 tig0020594:+21427 None:intergenic
AGCTCCGCTCCATGAAACAA+TGG 0.631104 tig0020594:+21487 None:intergenic
CTTACACCAAAACCAATGGA+AGG 0.637289 tig0020594:-21581 Msa1383330:CDS
TCAAATTGATGTGCCCGTGG+AGG 0.639975 tig0020594:-20348 Msa1383330:CDS
TGGAGCAACTTAATTTAAGG+CGG 0.645189 tig0020594:+21862 None:intergenic
TTACTGAACCAAGAGTTGGT+TGG 0.648299 tig0020594:-20410 Msa1383330:CDS
TCTTGTCACGAGCAGCAAGA+GGG 0.651954 tig0020594:-20575 Msa1383330:CDS
GCACAGAATAGAGAGTTGAA+AGG 0.656703 tig0020594:-20707 Msa1383330:CDS
ACTGGTGATTGTATATACGT+GGG 0.658637 tig0020594:-19074 Msa1383330:three_prime_UTR
TACTACAAATTACAAAACTG+AGG 0.659342 tig0020594:+19209 None:intergenic
TGGTGATTGTATATACGTGG+GGG 0.663825 tig0020594:-19072 Msa1383330:three_prime_UTR
AGGCGGTGGATAGAGTAGGA+AGG 0.671667 tig0020594:+21879 None:intergenic
GTAATTTGGGTGATGCTGTG+TGG 0.674970 tig0020594:-20439 Msa1383330:CDS
TAACAAAGTTCATACCAGCA+AGG 0.675115 tig0020594:+20200 None:intergenic
AATAACCACAGCCAAGCACG+AGG 0.677918 tig0020594:+19013 None:intergenic
TTGAAATGAAGCGAAAACGG+AGG 0.685238 tig0020594:-20682 Msa1383330:CDS
GCATTGAGTTCAGTTCCAAG+TGG 0.687922 tig0020594:-20470 Msa1383330:CDS
TTACTGTGTTTGAAGTAACG+AGG 0.690403 tig0020594:+21413 None:intergenic
GAATGGCTTTGTATGCAGCA+GGG 0.693731 tig0020594:-20095 Msa1383330:three_prime_UTR
GGTGATTGTATATACGTGGG+GGG 0.696287 tig0020594:-19071 Msa1383330:three_prime_UTR
GCTATGCAGCAAGAGACTGG+TGG 0.743962 tig0020594:-20959 Msa1383330:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATATAGTTAAAAACATT+AGG + tig0020594:20744-20763 None:intergenic 10.0%
!! TTGGATTAAAATATATATTA+AGG - tig0020594:21195-21214 Msa1383330:intron 10.0%
!! TGGATTAAAATATATATTAA+GGG - tig0020594:21196-21215 Msa1383330:intron 10.0%
!!! TTTGTTATTGTTATTGTTAT+TGG - tig0020594:20493-20512 Msa1383330:CDS 15.0%
!!! GGTTTGTTTATTTTAGTTTT+GGG + tig0020594:18871-18890 None:intergenic 20.0%
!!! TGGTTTGTTTATTTTAGTTT+TGG + tig0020594:18872-18891 None:intergenic 20.0%
!!! TCAATCTTTTATACAACAAT+TGG - tig0020594:19948-19967 Msa1383330:three_prime_UTR 20.0%
!! TGGTAGAAAATAATGATAAT+TGG - tig0020594:20355-20374 Msa1383330:CDS 20.0%
!! TTGATAAATAATTTCTGTGA+TGG - tig0020594:20542-20561 Msa1383330:CDS 20.0%
!! TAATTGATGTAGAATATGAA+TGG - tig0020594:20565-20584 Msa1383330:CDS 20.0%
!!! TGTTTTTAACTATATTTTGC+AGG - tig0020594:20746-20765 Msa1383330:CDS 20.0%
!! AATGCTTTATCCATAAATTT+GGG - tig0020594:21826-21845 Msa1383330:five_prime_UTR 20.0%
!!! TACAAACTATTCTAAAACTA+GGG + tig0020594:21883-21902 None:intergenic 20.0%
!!! TTACAAACTATTCTAAAACT+AGG + tig0020594:21884-21903 None:intergenic 20.0%
! AAATGGAGCAACTTAATTTA+AGG + tig0020594:18821-18840 None:intergenic 25.0%
! TATGAAAGGTAGTTATGTTA+TGG + tig0020594:18892-18911 None:intergenic 25.0%
! AAGTGATAGTTAACTTACAT+AGG + tig0020594:19319-19338 None:intergenic 25.0%
! TTGAATGTAATCATATGTGT+CGG + tig0020594:19456-19475 None:intergenic 25.0%
!! AATTTTTGTGTTCATATGCA+GGG - tig0020594:19583-19602 Msa1383330:three_prime_UTR 25.0%
! AAATAATGATAATTGGACTG+TGG - tig0020594:20362-20381 Msa1383330:CDS 25.0%
!! TGTTGTTTGGTTCAAAATAA+AGG + tig0020594:20644-20663 None:intergenic 25.0%
! CATTAGGATAGAATAAGAAA+TGG + tig0020594:20728-20747 None:intergenic 25.0%
! TCAATGCTTACAATTGAATA+TGG - tig0020594:21282-21301 Msa1383330:intron 25.0%
! TTGAGTGAATATTAGTGATT+TGG - tig0020594:21384-21403 Msa1383330:CDS 25.0%
! TACTACAAATTACAAAACTG+AGG + tig0020594:21471-21490 None:intergenic 25.0%
!!! TCAGTTTTGTAATTTGTAGT+AGG - tig0020594:21470-21489 Msa1383330:CDS 25.0%
!!! CAGTTTTGTAATTTGTAGTA+GGG - tig0020594:21471-21490 Msa1383330:CDS 25.0%
! TAATCACCATATAAAGATAC+AGG + tig0020594:21571-21590 None:intergenic 25.0%
! TGGTGTTTAAATAGACATAT+TGG - tig0020594:21783-21802 Msa1383330:five_prime_UTR 25.0%
! CAATGCTTTATCCATAAATT+TGG - tig0020594:21825-21844 Msa1383330:five_prime_UTR 25.0%
! ATGCTTTATCCATAAATTTG+GGG - tig0020594:21827-21846 Msa1383330:five_prime_UTR 25.0%
!! AGAGAAATTTGGTTTGGTAA+TGG + tig0020594:18762-18781 None:intergenic 30.0%
TGGGATTAGATAGATAGATT+GGG + tig0020594:18852-18871 None:intergenic 30.0%
TTGGGATTAGATAGATAGAT+TGG + tig0020594:18853-18872 None:intergenic 30.0%
!!! TCGATTTTGGAGCTTAATTT+TGG + tig0020594:18927-18946 None:intergenic 30.0%
!! TGTGATGTGATAATCGATTT+TGG + tig0020594:18940-18959 None:intergenic 30.0%
AAATCTTACACCAAAACCAA+TGG - tig0020594:19092-19111 Msa1383330:three_prime_UTR 30.0%
! TTCAACAACATCAAAGGTTT+TGG + tig0020594:19154-19173 None:intergenic 30.0%
!! TCGTAATAGCTTTGTTGTTT+GGG - tig0020594:19212-19231 Msa1383330:three_prime_UTR 30.0%
AAGAACAAATTCACAAACAC+CGG + tig0020594:19519-19538 None:intergenic 30.0%
! GAATTTTTGTGTTCATATGC+AGG - tig0020594:19582-19601 Msa1383330:three_prime_UTR 30.0%
AAATTACTCTCATTACCACT+TGG + tig0020594:20225-20244 None:intergenic 30.0%
! AAGTGGTAATGAGAGTAATT+TGG - tig0020594:20224-20243 Msa1383330:three_prime_UTR 30.0%
! AGTGGTAATGAGAGTAATTT+GGG - tig0020594:20225-20244 Msa1383330:three_prime_UTR 30.0%
!! GTTTTGAACCTTAGTCAAAT+TGG - tig0020594:20412-20431 Msa1383330:CDS 30.0%
TTCTACAACCAATTTGACTA+AGG + tig0020594:20423-20442 None:intergenic 30.0%
AATAAAGGATACACATGTGA+TGG + tig0020594:20629-20648 None:intergenic 30.0%
GTTCACAACAATATGTTGTT+TGG + tig0020594:20657-20676 None:intergenic 30.0%
GGATAGAATAAGAAATGGTA+TGG + tig0020594:20723-20742 None:intergenic 30.0%
TCAATCATCTTGACAACTTT+GGG + tig0020594:20777-20796 None:intergenic 30.0%
TTCAATCATCTTGACAACTT+TGG + tig0020594:20778-20797 None:intergenic 30.0%
AAGTTGTCAAGATGATTGAA+TGG - tig0020594:20778-20797 Msa1383330:CDS 30.0%
AGTTGTCAAGATGATTGAAT+GGG - tig0020594:20779-20798 Msa1383330:CDS 30.0%
ATGATTGAATGGGAAAAGTT+GGG - tig0020594:20789-20808 Msa1383330:CDS 30.0%
TTATCCTGGTCAAAGAAATT+TGG + tig0020594:21001-21020 None:intergenic 30.0%
CTAAAGATCAGAATTTATCC+TGG + tig0020594:21015-21034 None:intergenic 30.0%
GAATTGTAAATCGAATAGCT+TGG - tig0020594:21176-21195 Msa1383330:intron 30.0%
! TCTTGAGAGCATATATATGA+AGG - tig0020594:21228-21247 Msa1383330:intron 30.0%
! CTTGAGAGCATATATATGAA+GGG - tig0020594:21229-21248 Msa1383330:intron 30.0%
CATATATATGAAGGGTTGAA+GGG - tig0020594:21237-21256 Msa1383330:intron 30.0%
ATATGGTGATTAACTTCAAG+AGG - tig0020594:21579-21598 Msa1383330:CDS 30.0%
ATTCGTAATTCGTATCACTT+TGG - tig0020594:21734-21753 Msa1383330:five_prime_UTR 30.0%
!! TATGGATAAAGCATTGTCAA+CGG + tig0020594:21821-21840 None:intergenic 30.0%
TGGAGCAACTTAATTTAAGG+CGG + tig0020594:18818-18837 None:intergenic 35.0%
GGGATTAGATAGATAGATTG+GGG + tig0020594:18851-18870 None:intergenic 35.0%
!! GGAAAGTTGGTAAGTATGAA+AGG + tig0020594:18906-18925 None:intergenic 35.0%
!! GGAGCTTAATTTTGGAAAGT+TGG + tig0020594:18919-18938 None:intergenic 35.0%
AGCATCAACTAACTCAATGA+AGG - tig0020594:18981-19000 Msa1383330:three_prime_UTR 35.0%
GCATCAACTAACTCAATGAA+GGG - tig0020594:18982-19001 Msa1383330:three_prime_UTR 35.0%
TTAGCATATGTCAGTGTTGT+TGG - tig0020594:19030-19049 Msa1383330:three_prime_UTR 35.0%
TAACTCTTCATCTTCCTCTT+CGG - tig0020594:19065-19084 Msa1383330:three_prime_UTR 35.0%
!! TCAAAGGTTTTGGTGAGAAA+TGG + tig0020594:19144-19163 None:intergenic 35.0%
TGGATCTTCAACAACATCAA+AGG + tig0020594:19160-19179 None:intergenic 35.0%
!! CTCGTAATAGCTTTGTTGTT+TGG - tig0020594:19211-19230 Msa1383330:three_prime_UTR 35.0%
! TTACTGTGTTTGAAGTAACG+AGG + tig0020594:19267-19286 None:intergenic 35.0%
!!! GGGTTTTAGAAAAGTTGATC+CGG - tig0020594:19603-19622 Msa1383330:three_prime_UTR 35.0%
GTGGTGCTTGTATTGAATTA+GGG - tig0020594:19737-19756 Msa1383330:three_prime_UTR 35.0%
TGGTGCTTGTATTGAATTAG+GGG - tig0020594:19738-19757 Msa1383330:three_prime_UTR 35.0%
TGTATTGAATTAGGGGAGTA+TGG - tig0020594:19745-19764 Msa1383330:three_prime_UTR 35.0%
AATTATCTGCTATGGAAGCT+AGG - tig0020594:19863-19882 Msa1383330:three_prime_UTR 35.0%
GAGAATTTGCAAGATGATTC+TGG - tig0020594:20039-20058 Msa1383330:three_prime_UTR 35.0%
!! AAGATGATTCTGGAGCTATA+AGG - tig0020594:20049-20068 Msa1383330:three_prime_UTR 35.0%
!! AGATGATTCTGGAGCTATAA+GGG - tig0020594:20050-20069 Msa1383330:three_prime_UTR 35.0%
TAATCAACCACAGACTCAAT+TGG + tig0020594:20081-20100 None:intergenic 35.0%
TGAATCAAGCAGTGAAGTTA+AGG - tig0020594:20176-20195 Msa1383330:intron 35.0%
! ATTTTCTACCAAATCCTCCA+CGG + tig0020594:20346-20365 None:intergenic 35.0%
CAAAACCCATTTGATCAACA+AGG + tig0020594:20398-20417 None:intergenic 35.0%
TAACAAAGTTCATACCAGCA+AGG + tig0020594:20480-20499 None:intergenic 35.0%
GATGATTGAATGGGAAAAGT+TGG - tig0020594:20788-20807 Msa1383330:CDS 35.0%
! ATGATGTGTTGCTAGCAATT+CGG + tig0020594:20842-20861 None:intergenic 35.0%
AAACTCAAAAAAACTGTCGC+AGG + tig0020594:20895-20914 None:intergenic 35.0%
! CTCTTTTAGACTTGTCAAAG+AGG - tig0020594:20973-20992 Msa1383330:CDS 35.0%
!! TGGTTGTTTTGTTAATCTCG+CGG - tig0020594:21124-21143 Msa1383330:intron 35.0%
GCATATATATGAAGGGTTGA+AGG - tig0020594:21236-21255 Msa1383330:intron 35.0%
!!! GAACTGCAGCTTTATTTTTG+AGG - tig0020594:21406-21425 Msa1383330:CDS 35.0%
! TGGTGTTTTCCTCTTGTAAA+CGG - tig0020594:21510-21529 Msa1383330:CDS 35.0%
! AGTTGGCCTGTATCTTTATA+TGG - tig0020594:21562-21581 Msa1383330:CDS 35.0%
ACTGGTGATTGTATATACGT+GGG - tig0020594:21603-21622 Msa1383330:CDS 35.0%
ATTTAAACACCACACCATCT+GGG + tig0020594:21775-21794 None:intergenic 35.0%
TATTTAAACACCACACCATC+TGG + tig0020594:21776-21795 None:intergenic 35.0%
GGGTGAAGAGAAATTTGGTT+TGG + tig0020594:18768-18787 None:intergenic 40.0%
! AGCAACTTAATTTAAGGCGG+TGG + tig0020594:18815-18834 None:intergenic 40.0%
TAGATTGGGGAAGAGTGAAA+TGG + tig0020594:18838-18857 None:intergenic 40.0%
GAAGGGAATTAGAGTGAAAG+AGG - tig0020594:18999-19018 Msa1383330:three_prime_UTR 40.0%
AACTCTTCATCTTCCTCTTC+GGG - tig0020594:19066-19085 Msa1383330:three_prime_UTR 40.0%
TGTAAGATTTGAACCCGAAG+AGG + tig0020594:19082-19101 None:intergenic 40.0%
CTTACACCAAAACCAATGGA+AGG - tig0020594:19096-19115 Msa1383330:three_prime_UTR 40.0%
!!! TGCAATCCTTCCATTGGTTT+TGG + tig0020594:19105-19124 None:intergenic 40.0%
ATTTCGTGCAATCCTTCCAT+TGG + tig0020594:19111-19130 None:intergenic 40.0%
AATGGAAGGATTGCACGAAA+TGG - tig0020594:19110-19129 Msa1383330:three_prime_UTR 40.0%
ATGGAAGGATTGCACGAAAT+GGG - tig0020594:19111-19130 Msa1383330:three_prime_UTR 40.0%
!! AAGGTTTTGGTGAGAAATGG+AGG + tig0020594:19141-19160 None:intergenic 40.0%
GAAACAATGGAATCAGTGGA+TGG + tig0020594:19180-19199 None:intergenic 40.0%
CCATGAAACAATGGAATCAG+TGG + tig0020594:19184-19203 None:intergenic 40.0%
!! CCACTGATTCCATTGTTTCA+TGG - tig0020594:19181-19200 Msa1383330:three_prime_UTR 40.0%
GATTCCATTGTTTCATGGAG+CGG - tig0020594:19186-19205 Msa1383330:three_prime_UTR 40.0%
GTAACGAGGTAAAATAGCAG+TGG + tig0020594:19253-19272 None:intergenic 40.0%
CCAGACAATGTTGATGTATC+CGG - tig0020594:19497-19516 Msa1383330:three_prime_UTR 40.0%
! AAAAGTTGATCCGGATCGAT+GGG - tig0020594:19612-19631 Msa1383330:three_prime_UTR 40.0%
GGTGGTGCTTGTATTGAATT+AGG - tig0020594:19736-19755 Msa1383330:three_prime_UTR 40.0%
TGAGATTGAGAGGTTGAGAA+GGG - tig0020594:19777-19796 Msa1383330:three_prime_UTR 40.0%
TGGCTGAGATTGTGAAGTTA+AGG - tig0020594:19815-19834 Msa1383330:three_prime_UTR 40.0%
AAGGGAGCAATTATCTGCTA+TGG - tig0020594:19855-19874 Msa1383330:three_prime_UTR 40.0%
GCACAGAATAGAGAGTTGAA+AGG - tig0020594:19970-19989 Msa1383330:three_prime_UTR 40.0%
GCGTTGAAATGAAGCGAAAA+CGG - tig0020594:19992-20011 Msa1383330:three_prime_UTR 40.0%
TTGAAATGAAGCGAAAACGG+AGG - tig0020594:19995-20014 Msa1383330:three_prime_UTR 40.0%
GACTACTATTGAGTCTGAGA+TGG - tig0020594:20128-20147 Msa1383330:intron 40.0%
TAATTTGGGTGATGCTGTGT+GGG - tig0020594:20239-20258 Msa1383330:three_prime_UTR 40.0%
GGATTTACTGAACCAAGAGT+TGG - tig0020594:20263-20282 Msa1383330:exon 40.0%
! TTACTGAACCAAGAGTTGGT+TGG - tig0020594:20267-20286 Msa1383330:exon 40.0%
CCAATCACAACTTCATCTTC+TGG + tig0020594:20300-20319 None:intergenic 40.0%
CCAGAAGATGAAGTTGTGAT+TGG - tig0020594:20297-20316 Msa1383330:CDS 40.0%
! TTTTCTACCAAATCCTCCAC+GGG + tig0020594:20345-20364 None:intergenic 40.0%
!! CAGAGCCTTGTTGATCAAAT+GGG - tig0020594:20390-20409 Msa1383330:CDS 40.0%
CTAGCAACACATCATTCGTT+AGG - tig0020594:20847-20866 Msa1383330:CDS 40.0%
!! GGTATCTGGGTTTTGTCAAA+TGG + tig0020594:20874-20893 None:intergenic 40.0%
AAAAACTGTCGCAGGTATCT+GGG + tig0020594:20887-20906 None:intergenic 40.0%
AAAAAACTGTCGCAGGTATC+TGG + tig0020594:20888-20907 None:intergenic 40.0%
GTTCCCAAAAGGAACAGAAT+CGG + tig0020594:20921-20940 None:intergenic 40.0%
GGATCCAAATTTCTTTGACC+AGG - tig0020594:20994-21013 Msa1383330:CDS 40.0%
TGTGTTCTGTACATTGTTCG+CGG - tig0020594:21043-21062 Msa1383330:CDS 40.0%
! CAGTTTGCTTTCTTTGTTGC+TGG - tig0020594:21104-21123 Msa1383330:intron 40.0%
!! TTGTTTTGTTAATCTCGCGG+AGG - tig0020594:21127-21146 Msa1383330:intron 40.0%
GGATATGGTGTTCATGGAAT+TGG - tig0020594:21151-21170 Msa1383330:intron 40.0%
!!! CTGCTTTGTTGTTTTGCTGT+TGG - tig0020594:21306-21325 Msa1383330:intron 40.0%
!!! CTTTGTTGTTTTGCTGTTGG+TGG - tig0020594:21309-21328 Msa1383330:intron 40.0%
!!! TTGTTTTGCTGTTGGTGGAA+TGG - tig0020594:21314-21333 Msa1383330:intron 40.0%
!!! CTGCAGCTTTATTTTTGAGG+TGG - tig0020594:21409-21428 Msa1383330:CDS 40.0%
! CATTGTGAATTGAGTGTTGC+TGG - tig0020594:21432-21451 Msa1383330:CDS 40.0%
AGGGTCTCATTGTTATGTGT+TGG - tig0020594:21490-21509 Msa1383330:CDS 40.0%
! GGTGTTTTCCTCTTGTAAAC+GGG - tig0020594:21511-21530 Msa1383330:CDS 40.0%
! GTGTTTTCCTCTTGTAAACG+GGG - tig0020594:21512-21531 Msa1383330:CDS 40.0%
! TGATTAACTTCAAGAGGCAC+TGG - tig0020594:21585-21604 Msa1383330:CDS 40.0%
CACTGGTGATTGTATATACG+TGG - tig0020594:21602-21621 Msa1383330:CDS 40.0%
CTGGTGATTGTATATACGTG+GGG - tig0020594:21604-21623 Msa1383330:CDS 40.0%
TGGTGATTGTATATACGTGG+GGG - tig0020594:21605-21624 Msa1383330:CDS 40.0%
GAATCAACGCCCCAAATTTA+TGG + tig0020594:21839-21858 None:intergenic 40.0%
GAGACGGGTGAAGAGAAATT+TGG + tig0020594:18773-18792 None:intergenic 45.0%
!! AGTAGGAAGGTTCTAGAGAC+GGG + tig0020594:18788-18807 None:intergenic 45.0%
!! GAGTAGGAAGGTTCTAGAGA+CGG + tig0020594:18789-18808 None:intergenic 45.0%
! TTTAAGGCGGTGGATAGAGT+AGG + tig0020594:18805-18824 None:intergenic 45.0%
!! GTTTTGGTGAGAAATGGAGG+TGG + tig0020594:19138-19157 None:intergenic 45.0%
CCGGATACATCAACATTGTC+TGG + tig0020594:19500-19519 None:intergenic 45.0%
! GAAAAGTTGATCCGGATCGA+TGG - tig0020594:19611-19630 Msa1383330:three_prime_UTR 45.0%
!!! CGAACGAAGGGTTTTTAGCT+GGG - tig0020594:19641-19660 Msa1383330:three_prime_UTR 45.0%
! TTAGGGGAGTATGGTCTTGA+AGG - tig0020594:19754-19773 Msa1383330:three_prime_UTR 45.0%
GTCTTGAAGGTGAGATTGAG+AGG - tig0020594:19767-19786 Msa1383330:three_prime_UTR 45.0%
GTGAGATTGAGAGGTTGAGA+AGG - tig0020594:19776-19795 Msa1383330:three_prime_UTR 45.0%
!! AAGGGACAGGGCTGTTTTAA+TGG - tig0020594:19795-19814 Msa1383330:three_prime_UTR 45.0%
GCAGCAGCAGCATAATTCAA+GGG - tig0020594:19837-19856 Msa1383330:three_prime_UTR 45.0%
AACCACAGACTCAATTGGCA+CGG + tig0020594:20076-20095 None:intergenic 45.0%
GCATTGAGTTCAGTTCCAAG+TGG - tig0020594:20207-20226 Msa1383330:intron 45.0%
GTAATTTGGGTGATGCTGTG+TGG - tig0020594:20238-20257 Msa1383330:three_prime_UTR 45.0%
! TGAACCAAGAGTTGGTTGGT+GGG - tig0020594:20271-20290 Msa1383330:exon 45.0%
TTCTCAAATTGATGTGCCCG+TGG - tig0020594:20326-20345 Msa1383330:CDS 45.0%
!! GCAGAGCCTTGTTGATCAAA+TGG - tig0020594:20389-20408 Msa1383330:CDS 45.0%
!! TGAATGGCTTTGTATGCAGC+AGG - tig0020594:20581-20600 Msa1383330:CDS 45.0%
!! GAATGGCTTTGTATGCAGCA+GGG - tig0020594:20582-20601 Msa1383330:CDS 45.0%
GGAAGATTGTACTGCAGAAG+AGG - tig0020594:20603-20622 Msa1383330:CDS 45.0%
!! TCAGCCGATTCTGTTCCTTT+TGG - tig0020594:20914-20933 Msa1383330:CDS 45.0%
!! CAGCCGATTCTGTTCCTTTT+GGG - tig0020594:20915-20934 Msa1383330:CDS 45.0%
CAGAGATGACAGTTCCCAAA+AGG + tig0020594:20932-20951 None:intergenic 45.0%
!! CTTTTGGGAACTGTCATCTC+TGG - tig0020594:20930-20949 Msa1383330:CDS 45.0%
GTGTTCTGTACATTGTTCGC+GGG - tig0020594:21044-21063 Msa1383330:CDS 45.0%
! TTTTGTTAATCTCGCGGAGG+AGG - tig0020594:21130-21149 Msa1383330:intron 45.0%
!!! TTTTGCTGTTGGTGGAATGG+TGG - tig0020594:21317-21336 Msa1383330:intron 45.0%
!! TGTTGGTGGAATGGTGGATT+TGG - tig0020594:21323-21342 Msa1383330:intron 45.0%
! TGTGAATTGAGTGTTGCTGG+TGG - tig0020594:21435-21454 Msa1383330:CDS 45.0%
GGTGATTGTATATACGTGGG+GGG - tig0020594:21606-21625 Msa1383330:CDS 45.0%
CACGAGGAACAATAGGAGTA+TGG + tig0020594:21651-21670 None:intergenic 45.0%
!! TTGAAAAAAAAAAAAAAAAA+AGG + tig0020594:21694-21713 None:intergenic 5.0%
AGCTCCGCTCCATGAAACAA+TGG + tig0020594:19193-19212 None:intergenic 50.0%
TTCGCAAACTCCCATCGATC+CGG + tig0020594:19625-19644 None:intergenic 50.0%
GATGGGAGTTTGCGAACGAA+GGG - tig0020594:19629-19648 Msa1383330:three_prime_UTR 50.0%
!!! GCGAACGAAGGGTTTTTAGC+TGG - tig0020594:19640-19659 Msa1383330:three_prime_UTR 50.0%
!!! AAGGGTTTTTAGCTGGGCAG+AGG - tig0020594:19647-19666 Msa1383330:three_prime_UTR 50.0%
CAAGCTATGCAGCAAGAGAC+TGG - tig0020594:19715-19734 Msa1383330:three_prime_UTR 50.0%
TTGAGAGGTTGAGAAGGGAC+AGG - tig0020594:19782-19801 Msa1383330:three_prime_UTR 50.0%
TGAGAGGTTGAGAAGGGACA+GGG - tig0020594:19783-19802 Msa1383330:three_prime_UTR 50.0%
GGCAGCAGCAGCATAATTCA+AGG - tig0020594:19836-19855 Msa1383330:three_prime_UTR 50.0%
! GCTCACTGCTAGTTCGAGTT+TGG - tig0020594:20017-20036 Msa1383330:three_prime_UTR 50.0%
TCTTGTCACGAGCAGCAAGA+GGG - tig0020594:20102-20121 Msa1383330:three_prime_UTR 50.0%
! CTGAACCAAGAGTTGGTTGG+TGG - tig0020594:20270-20289 Msa1383330:exon 50.0%
GATTCCCACCAACCAACTCT+TGG + tig0020594:20278-20297 None:intergenic 50.0%
TCAAATTGATGTGCCCGTGG+AGG - tig0020594:20329-20348 Msa1383330:CDS 50.0%
! TCACTTGAGTTGCACCTTGC+TGG - tig0020594:20463-20482 Msa1383330:CDS 50.0%
TAATCTCGCGGAGGAGGATA+TGG - tig0020594:21136-21155 Msa1383330:intron 50.0%
GGAGGAGGATATGGTGTTCA+TGG - tig0020594:21145-21164 Msa1383330:intron 50.0%
CTGACAACCATCACTCCATC+AGG + tig0020594:21538-21557 None:intergenic 50.0%
CTGATGGAGTGATGGTTGTC+AGG - tig0020594:21536-21555 Msa1383330:CDS 50.0%
ATGGAGTGATGGTTGTCAGG+TGG - tig0020594:21539-21558 Msa1383330:CDS 50.0%
TGATGGTTGTCAGGTGGAGT+TGG - tig0020594:21545-21564 Msa1383330:CDS 50.0%
CTCCTATTGTTCCTCGTGCT+TGG - tig0020594:21653-21672 Msa1383330:CDS 50.0%
AGCCAAGCACGAGGAACAAT+AGG + tig0020594:21658-21677 None:intergenic 50.0%
AATAACCACAGCCAAGCACG+AGG + tig0020594:21667-21686 None:intergenic 50.0%
TACTTGCCATCTTGCCCAGA+TGG - tig0020594:21758-21777 Msa1383330:five_prime_UTR 50.0%
AGGCGGTGGATAGAGTAGGA+AGG + tig0020594:18801-18820 None:intergenic 55.0%
CGATGGGAGTTTGCGAACGA+AGG - tig0020594:19628-19647 Msa1383330:three_prime_UTR 55.0%
GCTATGCAGCAAGAGACTGG+TGG - tig0020594:19718-19737 Msa1383330:three_prime_UTR 55.0%
CTCTTGTCACGAGCAGCAAG+AGG - tig0020594:20101-20120 Msa1383330:three_prime_UTR 55.0%
TTTGGGTGATGCTGTGTGGG+AGG - tig0020594:20242-20261 Msa1383330:three_prime_UTR 55.0%
TGATGTGCCCGTGGAGGATT+TGG - tig0020594:20335-20354 Msa1383330:CDS 55.0%
CATTGTTCGCGGGTCAGTCT+AGG - tig0020594:21054-21073 Msa1383330:CDS 55.0%
CATCAGGCCCCGTTTACAAG+AGG + tig0020594:21522-21541 None:intergenic 55.0%
CTCTTGTAAACGGGGCCTGA+TGG - tig0020594:21520-21539 Msa1383330:CDS 55.0%
TTGTTCCTCGTGCTTGGCTG+TGG - tig0020594:21659-21678 Msa1383330:CDS 55.0%
ACCACACCATCTGGGCAAGA+TGG + tig0020594:21767-21786 None:intergenic 55.0%
GGCCGTGCCAATTGAGTCTG+TGG - tig0020594:20071-20090 Msa1383330:three_prime_UTR 60.0%
AACGGGGCCTGATGGAGTGA+TGG - tig0020594:21528-21547 Msa1383330:CDS 60.0%
GCCATCTTGCCCAGATGGTG+TGG - tig0020594:21763-21782 Msa1383330:five_prime_UTR 60.0%
Chromosome Type Strat End Strand Name
tig0020594 gene 18749 21950 18749 ID=Msa1383330;Name=Msa1383330
tig0020594 mRNA 18749 21950 18749 ID=Msa1383330-mRNA-1;Parent=Msa1383330;Name=Msa1383330-mRNA-1;_AED=0.02;_eAED=0.02;_QI=248|1|1|1|0.5|0.33|3|1433|381
tig0020594 exon 18749 20122 18749 ID=Msa1383330-mRNA-1:exon:8553;Parent=Msa1383330-mRNA-1
tig0020594 exon 20215 21095 20215 ID=Msa1383330-mRNA-1:exon:8552;Parent=Msa1383330-mRNA-1
tig0020594 exon 21379 21950 21379 ID=Msa1383330-mRNA-1:exon:8551;Parent=Msa1383330-mRNA-1
tig0020594 five_prime_UTR 21703 21950 21703 ID=Msa1383330-mRNA-1:five_prime_utr;Parent=Msa1383330-mRNA-1
tig0020594 CDS 21379 21702 21379 ID=Msa1383330-mRNA-1:cds;Parent=Msa1383330-mRNA-1
tig0020594 CDS 20274 21095 20274 ID=Msa1383330-mRNA-1:cds;Parent=Msa1383330-mRNA-1
tig0020594 three_prime_UTR 20215 20273 20215 ID=Msa1383330-mRNA-1:three_prime_utr;Parent=Msa1383330-mRNA-1
tig0020594 three_prime_UTR 18749 20122 18749 ID=Msa1383330-mRNA-1:three_prime_utr;Parent=Msa1383330-mRNA-1
Gene Sequence

>Msa1383330

ATGAAGGGAATTAGAGTGAAAGAGGAAGAAACATTAGCATATGTCAGTGTTGTTGGTTCAACATCATGTAACTCTTCATCTTCCTCTTCGGGTTCAAATCTTACACCAAAACCAATGGAAGGATTGCACGAAATGGGTCCACCTCCATTTCTCACCAAAACCTTTGATGTTGTTGAAGATCCATCCACTGATTCCATTGTTTCATGGAGCGGAGCTCGTAATAGCTTTGTTGTTTGGGATCTTCACAAATTCTCCACTGCTATTTTACCTCGTTACTTCAAACACAGTAATTTCTCTAGCTTCGTTCGACAACTCAATACCTATGGTTTTAGAAAAGTTGATCCGGATCGATGGGAGTTTGCGAACGAAGGGTTTTTAGCTGGGCAGAGGAACTTGTTGAAAACAATCAAGAGAAGAAGAAATGTGACACAATCACAAGCTATGCAGCAAGAGACTGGTGGTGCTTGTATTGAATTAGGGGAGTATGGTCTTGAAGGTGAGATTGAGAGGTTGAGAAGGGACAGGGCTGTTTTAATGGCTGAGATTGTGAAGTTAAGGCAGCAGCAGCATAATTCAAGGGAGCAATTATCTGCTATGGAAGCTAGGTTGTTGATTACTGAGAAGAAACATCAACAGATGATGAATTTTCTTGCAAAAGCACTTAGCAATCAATCTTTTATACAACAATTGGCACAGAATAGAGAGTTGAAAGGCGTTGAAATGAAGCGAAAACGGAGGCTCACTGCTAGTTCGAGTTTGGAGAATTTGCAAGATGATTCTGGAGCTATAAGGGCCGTGCCAATTGAGTCTGTGGTTGATTACTCTTGTCACGAGCAGCAAGAGGGTTTGACTACTATTGAGTCTGAGATGGAGACACTGTTAAGTGCTTATGATAATGAATCAAGCAGTGAAGTTAAGGACTACAGTGCATTGAGTTCAGTTCCAAGTGGTAATGAGAGTAATTTGGGTGATGCTGTGTGGGAGGATTTACTGAACCAAGAGTTGGTTGGTGGGAATCCAGAAGATGAAGTTGTGATTGGTGATTTTTCTCAAATTGATGTGCCCGTGGAGGATTTGGTAGAAAATAATGATAATTGGACTGTGGATTTGCAGAGCCTTGTTGATCAAATGGGTTTTGAACCTTAG
Protein sequence

>Msa1383330

MKGIRVKEEETLAYVSVVGSTSCNSSSSSSGSNLTPKPMEGLHEMGPPPFLTKTFDVVEDPSTDSIVSWSGARNSFVVWDLHKFSTAILPRYFKHSNFSSFVRQLNTYGFRKVDPDRWEFANEGFLAGQRNLLKTIKRRRNVTQSQAMQQETGGACIELGEYGLEGEIERLRRDRAVLMAEIVKLRQQQHNSREQLSAMEARLLITEKKHQQMMNFLAKALSNQSFIQQLAQNRELKGVEMKRKRRLTASSSLENLQDDSGAIRAVPIESVVDYSCHEQQEGLTTIESEMETLLSAYDNESSSEVKDYSALSSVPSGNESNLGDAVWEDLLNQELVGGNPEDEVVIGDFSQIDVPVEDLVENNDNWTVDLQSLVDQMGFEP*