AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1388710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1388710 MtrunA17_Chr7g0273721 93.939 462 27 1 1 461 1 462 0.0 911
Msa1388710 MtrunA17_Chr5g0433641 86.928 459 60 0 3 461 2 460 0.0 852
Msa1388710 MtrunA17_Chr7g0273791 77.851 456 100 1 3 458 2 456 0.0 761
Msa1388710 MtrunA17_Chr7g0273781 76.790 461 105 2 1 461 34 492 0.0 756
Msa1388710 MtrunA17_Chr7g0273761 75.541 462 112 1 1 461 372 833 0.0 741
Msa1388710 MtrunA17_Chr7g0273761 71.197 309 89 0 148 456 32 340 3.34e-157 466
Msa1388710 MtrunA17_Chr7g0273731 75.839 447 107 1 1 447 83 528 0.0 732
Msa1388710 MtrunA17_Chr1g0165671 60.349 459 174 5 7 461 23 477 0.0 573
Msa1388710 MtrunA17_Chr1g0165681 54.113 462 200 8 7 461 5 461 2.03e-178 507
Msa1388710 MtrunA17_Chr1g0165661 54.466 459 200 6 7 461 5 458 1.12e-177 505
Msa1388710 MtrunA17_Chr1g0191081 56.858 452 180 7 3 447 9 452 5.95e-176 500
Msa1388710 MtrunA17_Chr1g0165711 51.310 458 175 7 7 461 5 417 7.03e-154 443
Msa1388710 MtrunA17_Chr7g0273701 89.080 174 19 0 288 461 1 174 1.37e-114 334
Msa1388710 MtrunA17_Chr7g0273741 81.633 147 27 0 3 149 2 148 4.80e-83 252
Msa1388710 MtrunA17_Chr6g0471381 51.765 255 117 5 199 450 6 257 6.80e-83 256
Msa1388710 MtrunA17_Chr8g0386141 35.476 389 237 8 9 392 5 384 1.95e-80 256
Msa1388710 MtrunA17_Chr3g0132001 33.178 428 270 8 11 435 14 428 4.57e-79 252
Msa1388710 MtrunA17_Chr7g0224391 34.794 388 239 8 14 396 19 397 6.51e-76 244
Msa1388710 MtrunA17_Chr3g0098961 72.483 149 41 0 1 149 3 151 2.28e-73 229
Msa1388710 MtrunA17_Chr6g0471371 56.571 175 67 1 7 181 5 170 8.69e-66 209
Msa1388710 MtrunA17_Chr1g0165741 63.636 143 52 0 7 149 5 147 1.40e-62 199
Msa1388710 MtrunA17_Chr6g0471971 46.639 238 100 6 163 397 1 214 2.10e-61 199
Msa1388710 MtrunA17_Chr2g0306321 30.805 435 284 11 19 439 26 457 1.15e-59 202
Msa1388710 MtrunA17_Chr8g0353421 63.780 127 46 0 4 130 2 128 2.72e-57 185
Msa1388710 MtrunA17_Chr3g0141851 31.888 392 241 11 21 400 22 399 1.47e-56 194
Msa1388710 MtrunA17_Chr7g0273691 87.368 95 12 0 167 261 1 95 2.52e-56 181
Msa1388710 MtrunA17_Chr2g0334001 29.241 448 289 14 7 442 1 432 4.37e-55 189
Msa1388710 MtrunA17_Chr4g0027071 30.493 446 274 16 18 439 19 452 3.33e-52 182
Msa1388710 MtrunA17_Chr4g0001861 29.117 419 259 14 43 442 34 433 1.84e-50 177
Msa1388710 MtrunA17_Chr8g0371821 27.088 443 295 13 7 439 1 425 8.54e-46 164
Msa1388710 MtrunA17_Chr3g0113821 29.750 400 261 10 10 396 4 396 1.15e-45 164
Msa1388710 MtrunA17_Chr2g0283361 31.778 343 221 7 56 391 48 384 6.39e-44 160
Msa1388710 MtrunA17_Chr7g0270771 31.333 300 163 8 7 293 1 270 1.00e-42 152
Msa1388710 MtrunA17_Chr7g0253501 27.952 415 253 13 18 394 9 415 7.77e-39 146
Msa1388710 MtrunA17_Chr7g0253531 28.434 415 253 15 17 394 8 415 1.05e-38 146
Msa1388710 MtrunA17_Chr6g0471891 42.408 191 82 4 234 422 1 165 1.39e-38 139
Msa1388710 MtrunA17_Chr7g0252731 28.311 438 273 16 4 429 8 416 2.55e-38 144
Msa1388710 MtrunA17_Chr4g0072041 27.938 451 279 14 19 439 22 456 2.87e-38 144
Msa1388710 MtrunA17_Chr3g0087401 25.747 435 258 12 19 415 10 417 3.16e-37 141
Msa1388710 MtrunA17_Chr4g0054081 27.294 436 267 14 37 439 34 452 2.22e-36 139
Msa1388710 MtrunA17_Chr2g0311401 29.221 308 196 10 147 442 5 302 4.82e-36 135
Msa1388710 MtrunA17_Chr7g0253541 25.000 468 289 18 19 443 12 460 1.91e-34 134
Msa1388710 MtrunA17_Chr6g0470591 27.723 404 249 15 65 439 70 459 6.36e-33 130
Msa1388710 MtrunA17_Chr3g0106691 26.786 392 263 11 18 391 63 448 1.03e-32 130
Msa1388710 MtrunA17_Chr3g0106661 27.983 461 277 18 18 439 17 461 1.52e-30 123
Msa1388710 MtrunA17_Chr5g0442691 26.128 421 261 14 9 393 31 437 3.26e-30 122
Msa1388710 MtrunA17_Chr5g0442701 26.128 421 261 14 9 393 8 414 1.98e-29 120
Msa1388710 MtrunA17_Chr1g0165721 50.909 110 52 2 349 456 1 110 4.59e-29 110
Msa1388710 MtrunA17_Chr7g0235531 25.000 444 288 14 18 437 11 433 1.06e-28 117
Msa1388710 MtrunA17_Chr3g0106721 29.730 296 183 9 61 341 63 348 8.34e-28 115
Msa1388710 MtrunA17_Chr7g0273681 86.538 52 7 0 93 144 1 52 1.14e-26 101
Msa1388710 MtrunA17_Chr3g0106711 33.508 191 115 5 18 199 17 204 3.10e-26 106
Msa1388710 MtrunA17_Chr1g0165731 44.737 114 56 4 163 273 1 110 4.70e-26 102
Msa1388710 MtrunA17_Chr5g0442721 26.764 411 250 16 63 438 64 458 8.63e-26 109
Msa1388710 MtrunA17_Chr4g0004691 33.721 172 100 5 19 186 12 173 1.46e-25 103
Msa1388710 MtrunA17_Chr3g0113181 24.299 428 256 12 54 435 54 459 4.84e-25 107
Msa1388710 MtrunA17_Chr3g0134091 26.097 433 269 16 25 432 30 436 2.11e-24 105
Msa1388710 MtrunA17_Chr3g0134121 26.526 426 275 17 26 432 31 437 4.82e-24 104
Msa1388710 MtrunA17_Chr5g0408911 25.058 431 273 15 24 430 33 437 5.15e-24 104
Msa1388710 MtrunA17_Chr3g0106761 25.915 328 217 10 81 391 29 347 6.12e-24 103
Msa1388710 MtrunA17_Chr3g0106741 26.300 327 217 9 81 391 60 378 6.28e-23 100
Msa1388710 MtrunA17_Chr3g0134041 25.990 404 247 16 66 442 66 444 6.60e-23 100
Msa1388710 MtrunA17_Chr3g0095731 23.641 423 252 14 44 428 43 432 2.05e-22 99.8
Msa1388710 MtrunA17_Chr3g0123061 23.982 442 273 19 17 439 11 408 1.05e-21 97.1
Msa1388710 MtrunA17_Chr3g0095721 23.441 401 239 13 63 428 69 436 3.58e-21 95.9
Msa1388710 MtrunA17_Chr8g0354371 24.561 399 264 13 25 405 31 410 5.49e-21 95.1
Msa1388710 MtrunA17_Chr3g0134111 24.885 434 271 16 26 432 32 437 7.35e-21 94.7
Msa1388710 MtrunA17_Chr3g0134071 25.062 403 245 18 38 405 44 424 7.76e-21 95.1
Msa1388710 MtrunA17_Chr3g0125001 24.362 431 259 19 25 428 25 415 9.57e-21 94.4
Msa1388710 MtrunA17_Chr5g0438701 24.201 438 258 21 17 422 9 404 2.68e-20 93.2
Msa1388710 MtrunA17_Chr4g0010381 24.711 433 262 19 25 428 25 422 2.04e-19 90.5
Msa1388710 MtrunA17_Chr3g0134061 24.299 428 268 17 38 432 42 446 6.43e-19 89.0
Msa1388710 MtrunA17_Chr8g0374641 23.953 430 269 19 25 428 30 427 1.35e-18 87.8
Msa1388710 MtrunA17_Chr4g0053111 24.256 437 262 17 25 427 28 429 2.27e-18 87.4
Msa1388710 MtrunA17_Chr4g0010331 23.218 435 271 16 25 433 25 422 1.20e-17 85.1
Msa1388710 MtrunA17_Chr7g0273671 88.636 44 4 1 1 44 1 43 2.01e-17 75.9
Msa1388710 MtrunA17_Chr1g0199561 24.221 417 255 16 17 399 9 398 3.59e-17 83.6
Msa1388710 MtrunA17_Chr4g0053061 23.598 428 276 18 26 428 59 460 6.26e-17 83.2
Msa1388710 MtrunA17_Chr8g0374911 24.009 429 273 19 26 428 52 453 8.90e-17 82.4
Msa1388710 MtrunA17_Chr4g0053091 22.794 408 253 14 25 405 32 404 1.09e-16 82.0
Msa1388710 MtrunA17_Chr6g0471981 60.784 51 20 0 102 152 1 51 2.95e-16 73.2
Msa1388710 MtrunA17_Chr8g0361771 23.961 409 261 19 17 399 9 393 1.09e-15 79.0
Msa1388710 MtrunA17_Chr4g0053081 22.222 432 276 18 25 430 33 430 3.20e-15 77.4
Msa1388710 MtrunA17_Chr8g0359251 22.406 424 284 15 25 430 30 426 1.60e-14 75.5
Msa1388710 MtrunA17_Chr1g0169471 38.384 99 53 2 91 188 13 104 1.85e-14 69.7
Msa1388710 MtrunA17_Chr4g0010121 23.192 401 254 15 25 401 70 440 2.27e-14 75.1
Msa1388710 MtrunA17_Chr5g0414431 55.357 56 20 1 53 108 2 52 1.36e-13 65.1
Msa1388710 MtrunA17_Chr3g0087901 22.222 432 273 15 46 435 46 456 1.49e-13 72.4
Msa1388710 MtrunA17_Chr7g0252771 25.490 204 142 5 194 392 7 205 2.71e-13 69.7
Msa1388710 MtrunA17_Chr6g0456591 28.846 156 100 6 61 208 64 216 9.83e-13 67.8
Msa1388710 MtrunA17_Chr3g0106631 26.761 142 96 4 149 285 2 140 1.29e-11 62.8
Msa1388710 MtrunA17_Chr2g0311411 29.730 148 98 4 7 154 1 142 7.63e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1388710 AT5G17540.1 48.817 465 226 8 4 461 2 461 1.54e-149 434
Msa1388710 AT3G03480.1 47.427 447 225 7 2 444 10 450 2.56e-138 405
Msa1388710 AT3G48720.1 32.832 399 256 8 1 396 1 390 5.39e-76 244
Msa1388710 AT5G41040.1 34.937 395 237 8 11 396 33 416 3.79e-75 243
Msa1388710 AT5G41040.2 34.937 395 237 8 11 396 17 400 4.95e-75 242
Msa1388710 AT5G63560.1 35.787 394 229 9 11 396 9 386 5.21e-75 241
Msa1388710 AT1G27620.1 30.070 429 274 10 14 435 12 421 7.62e-59 200
Msa1388710 AT1G03390.1 30.201 447 288 14 9 441 23 459 4.27e-56 193
Msa1388710 AT5G48930.1 28.440 436 283 13 18 439 10 430 1.85e-48 172
Msa1388710 AT2G19070.1 29.630 459 278 14 10 439 5 447 1.37e-46 167
Msa1388710 AT3G62160.1 29.950 404 247 11 7 398 1 380 1.49e-46 167
Msa1388710 AT2G40230.1 29.500 400 256 12 11 396 5 392 9.77e-45 162
Msa1388710 AT5G57840.1 27.609 460 284 16 11 444 3 439 1.52e-42 156
Msa1388710 AT1G28680.1 30.189 371 238 12 32 391 27 387 6.88e-42 155
Msa1388710 AT5G07080.1 29.854 412 254 13 1 391 6 403 1.40e-41 154
Msa1388710 AT1G65450.1 27.851 456 299 15 4 443 8 449 3.01e-41 153
Msa1388710 AT3G47170.1 27.521 476 285 17 9 443 10 466 7.04e-39 147
Msa1388710 AT1G28680.2 29.891 368 230 13 32 381 27 384 3.34e-38 143
Msa1388710 AT2G25150.1 28.330 473 276 17 6 442 7 452 3.18e-35 136
Msa1388710 AT2G23510.1 28.916 415 246 17 3 391 7 398 1.93e-34 134
Msa1388710 AT1G31490.1 24.020 408 264 14 8 393 3 386 2.09e-26 111
Msa1388710 AT5G42830.1 27.727 440 261 18 21 433 27 436 1.05e-25 109
Msa1388710 AT4G31910.1 26.483 472 295 21 6 441 2 457 1.57e-25 108
Msa1388710 AT4G15390.1 25.450 444 277 20 17 435 14 428 1.75e-25 108
Msa1388710 AT4G15390.2 25.450 444 277 20 17 435 14 428 1.75e-25 108
Msa1388710 AT5G07860.1 25.823 395 242 15 63 433 69 436 3.23e-23 102
Msa1388710 AT3G50280.1 25.862 406 255 16 25 409 28 408 1.25e-22 100
Msa1388710 AT3G50270.1 25.121 414 267 13 25 417 30 421 1.99e-22 99.8
Msa1388710 AT3G26040.1 23.457 405 253 15 17 394 9 383 4.99e-22 98.6
Msa1388710 AT1G78990.1 24.798 371 243 11 44 394 45 399 1.95e-21 97.1
Msa1388710 AT1G24430.1 25.400 437 275 18 17 435 11 414 2.33e-21 96.7
Msa1388710 AT5G38130.1 25.679 405 257 15 25 410 48 427 3.24e-21 96.3
Msa1388710 AT5G07850.1 24.053 449 269 19 21 433 28 440 1.21e-20 94.7
Msa1388710 AT1G24420.2 25.390 449 264 18 17 435 11 418 1.38e-20 94.4
Msa1388710 AT1G24420.1 25.390 449 264 18 17 435 11 418 1.38e-20 94.4
Msa1388710 AT1G32910.1 23.333 390 248 12 64 431 63 423 6.00e-20 92.4
Msa1388710 AT1G65450.3 26.351 296 196 10 159 443 1 285 8.83e-20 89.7
Msa1388710 AT3G30280.1 24.434 442 277 19 17 432 12 422 1.03e-19 91.7
Msa1388710 AT5G47980.1 25.446 448 278 18 15 436 9 426 5.87e-19 89.4
Msa1388710 AT2G39980.1 23.628 419 254 12 66 432 62 466 7.60e-19 89.4
Msa1388710 AT5G47950.1 24.138 435 270 19 17 432 12 405 3.28e-18 87.0
Msa1388710 AT5G23970.1 25.901 444 262 19 17 435 9 410 7.41e-18 85.9
Msa1388710 AT5G16410.1 23.699 346 233 12 66 394 91 422 9.05e-18 85.9
Msa1388710 AT3G50300.1 23.784 370 244 12 63 410 56 409 1.11e-15 79.3
Msa1388710 AT4G15400.1 26.126 444 272 20 17 438 11 420 7.03e-15 77.0
Msa1388710 AT5G23940.1 24.088 411 266 16 53 437 53 443 9.20e-15 76.6
Msa1388710 AT3G50280.2 24.113 282 189 10 140 409 10 278 7.65e-13 69.7

Find 150 sgRNAs with CRISPR-Local

Find 265 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACACTTCTTAACTCTATT+TGG 0.145351 tig0021157:+88042 Msa1388710:five_prime_UTR
ATCTTATCACTGCATGTTAT+TGG 0.178748 tig0021157:+90691 Msa1388710:CDS
CACTCCTTGTAGGATGATTT+TGG 0.205974 tig0021157:-91235 None:intergenic
TCTTCCTTGATGTACGTATT+TGG 0.224399 tig0021157:-90564 None:intergenic
TGGGGTGTGGATAGGGTTGA+TGG 0.279515 tig0021157:+91429 Msa1388710:three_prime_UTR
TGTGTCTTTGGTCATGGTTT+TGG 0.289871 tig0021157:+91525 Msa1388710:three_prime_UTR
GATATAACACTTGATCAATT+TGG 0.295091 tig0021157:+88418 Msa1388710:CDS
TTGTTGCTTGTCGGATATTA+TGG 0.296854 tig0021157:+90807 Msa1388710:CDS
GCATGTTTAAGAACCTTAAC+AGG 0.298889 tig0021157:-88277 None:intergenic
AGACGTGAACGGTGTGTAAC+AGG 0.307672 tig0021157:+91652 Msa1388710:three_prime_UTR
ACAAACCTCTTCATATCTTC+AGG 0.311975 tig0021157:-91185 None:intergenic
AACTCAACTGCATAACCTAA+TGG 0.312827 tig0021157:-90885 None:intergenic
GGGTCTTTCTCTTTCATTGA+TGG 0.316385 tig0021157:-88256 None:intergenic
CCATTCAACCTGTATGGAAT+AGG 0.323735 tig0021157:+90472 Msa1388710:CDS
GAACATCGTATAGAATTTCT+TGG 0.334851 tig0021157:-88471 None:intergenic
CTGCCGCACAAAAGCATTGC+AGG 0.340219 tig0021157:+90716 Msa1388710:CDS
TTCTTATTTGGGGTGTGGAT+AGG 0.343255 tig0021157:+91421 Msa1388710:three_prime_UTR
TCTTATTTGGGGTGTGGATA+GGG 0.353820 tig0021157:+91422 Msa1388710:three_prime_UTR
TGGTAATTGTATTGCTTTCT+CGG 0.356387 tig0021157:+90827 Msa1388710:CDS
AGAGGAGTACATGCATTCTT+TGG 0.363021 tig0021157:+90935 Msa1388710:CDS
ATCCAGCTGCAGGAAGGATT+AGG 0.365308 tig0021157:+88326 Msa1388710:CDS
ATCACAAGCTACAAACTATA+AGG 0.369159 tig0021157:-91279 None:intergenic
CTTGACTGCATGGGCAGAAA+TGG 0.369373 tig0021157:+90428 Msa1388710:CDS
GAGCTTTGTGGAAACCCATT+AGG 0.375078 tig0021157:+90870 Msa1388710:CDS
AAGTTCTCAGAAGTAAATTT+CGG 0.389814 tig0021157:+91035 Msa1388710:CDS
TCCAGCTGCAGGAAGGATTA+GGG 0.391602 tig0021157:+88327 Msa1388710:CDS
AACAAATGAGAAAAGATTAG+AGG 0.400929 tig0021157:-91458 None:intergenic
TCCCTAATCCTTCCTGCAGC+TGG 0.402307 tig0021157:-88328 None:intergenic
AAACTAGGAGCACTCCTTGT+AGG 0.407155 tig0021157:-91245 None:intergenic
GGGTGTGGATAGGGTTGATG+GGG 0.407349 tig0021157:+91431 Msa1388710:three_prime_UTR
CATTCAACCTGTATGGAATA+GGG 0.408225 tig0021157:+90473 Msa1388710:CDS
GCTAACCCTTTGTTGCTTGT+CGG 0.409807 tig0021157:+90798 Msa1388710:CDS
TTTCTTGGAAGCAAGGGAAT+GGG 0.413240 tig0021157:-88456 None:intergenic
GTGTATGGTGGAGCAGTAAA+AGG 0.414611 tig0021157:+91071 Msa1388710:CDS
GGGTGCTGGGCGAAAGTTGA+TGG 0.420210 tig0021157:+88351 Msa1388710:CDS
AACCTTCCATTCAACCTGTA+TGG 0.427456 tig0021157:+90466 Msa1388710:CDS
TCATGAGGAGTGGGTGCAGC+TGG 0.430177 tig0021157:-88154 None:intergenic
CAGCTCCATCGCCCATTGTA+TGG 0.433098 tig0021157:-90392 None:intergenic
GGAAGGATTAGGGAGGGTGC+TGG 0.433324 tig0021157:+88337 Msa1388710:CDS
ACTCAACTGCATAACCTAAT+GGG 0.436624 tig0021157:-90884 None:intergenic
GAAAGATGTTTATTTACAAA+GGG 0.441292 tig0021157:+90978 Msa1388710:CDS
GAAATTCTATACGATGTTCC+AGG 0.443086 tig0021157:+88475 Msa1388710:CDS
CTGTGAAGAAGAAAGATTGA+TGG 0.446115 tig0021157:-90605 None:intergenic
GGAAAGATGTTTATTTACAA+AGG 0.447433 tig0021157:+90977 Msa1388710:CDS
CCTATTCCATACAGGTTGAA+TGG 0.453015 tig0021157:-90472 None:intergenic
ATCATTCGTACCTCTTCATC+TGG 0.459290 tig0021157:-90744 None:intergenic
AAGAATGCAAAAGGAGAAGA+AGG 0.460427 tig0021157:+91137 Msa1388710:CDS
ATTTCTTGGAAGCAAGGGAA+TGG 0.473618 tig0021157:-88457 None:intergenic
CATAGCCGCGATTCGTCGCC+TGG 0.474719 tig0021157:+90635 Msa1388710:CDS
TACAAAGGGAAGAACTTGTA+TGG 0.478317 tig0021157:+90992 Msa1388710:CDS
AGAATCTCCCTATTCCATAC+AGG 0.480630 tig0021157:-90480 None:intergenic
ATGGTGGATTGCACTGGGGA+GGG 0.483447 tig0021157:+88370 Msa1388710:CDS
CAAACCTCTTCATATCTTCA+GGG 0.484202 tig0021157:-91184 None:intergenic
GCATCGTTAATGCACGTTCG+AGG 0.486409 tig0021157:+90769 Msa1388710:CDS
TCAATAATATATTCTGATCC+TGG 0.488870 tig0021157:-88493 None:intergenic
TCCTTGATGTACGTATTTGG+CGG 0.490692 tig0021157:-90561 None:intergenic
AAGACCTGTATGAGTCGAAC+GGG 0.490760 tig0021157:-88520 None:intergenic
CAAGTGTGGTGGTTTCATAA+TGG 0.491746 tig0021157:+90356 Msa1388710:CDS
GAACTTGTATGGTGTCAGAT+TGG 0.493406 tig0021157:+91003 Msa1388710:CDS
GTTTATTACTATCCAGCTGC+AGG 0.495084 tig0021157:+88316 Msa1388710:CDS
TGAATCATTTATATATCAAT+CGG 0.496551 tig0021157:-91305 None:intergenic
GGTGTGGATAGGGTTGATGG+GGG 0.496722 tig0021157:+91432 Msa1388710:three_prime_UTR
TGGAAGTACCAACTCTGGTT+GGG 0.497528 tig0021157:-88134 None:intergenic
GGATGGGGTGAAGCTGTGTA+TGG 0.499977 tig0021157:+91056 Msa1388710:CDS
ATGGAATAGGGAGATTCTAA+TGG 0.500842 tig0021157:+90485 Msa1388710:CDS
GAGAAAGGTGAAGTGGAACC+AGG 0.501342 tig0021157:-90653 None:intergenic
GTCCCGCATAAGAATGCAAA+AGG 0.502043 tig0021157:+91128 Msa1388710:CDS
GGGGTGTGGATAGGGTTGAT+GGG 0.505527 tig0021157:+91430 Msa1388710:three_prime_UTR
CATACAATGGGCGATGGAGC+TGG 0.513365 tig0021157:+90393 Msa1388710:CDS
GATGGTGGATTGCACTGGGG+AGG 0.517131 tig0021157:+88369 Msa1388710:CDS
CTGGAAGTACCAACTCTGGT+TGG 0.519392 tig0021157:-88135 None:intergenic
TATAGAATTTCTTGGAAGCA+AGG 0.520340 tig0021157:-88463 None:intergenic
TTCCATACAGGTTGAATGGA+AGG 0.520405 tig0021157:-90468 None:intergenic
GAAGGATTAGGGAGGGTGCT+GGG 0.522109 tig0021157:+88338 Msa1388710:CDS
CGTATGCAGTGCATTGAGAA+AGG 0.526515 tig0021157:-90668 None:intergenic
ATTCTTATGCGGGACAAGAA+AGG 0.526948 tig0021157:-91120 None:intergenic
AGTTTAACTTCATGAGGAGT+GGG 0.527780 tig0021157:-88163 None:intergenic
AAGTTGATGGTGGATTGCAC+TGG 0.528428 tig0021157:+88364 Msa1388710:CDS
GGAGCAGTAAAAGGTGGAGT+TGG 0.530628 tig0021157:+91080 Msa1388710:CDS
GAAAGAGAAAGACCCTGTTA+AGG 0.531454 tig0021157:+88264 Msa1388710:CDS
GGTTGCAAGTAACGTGTGGC+GGG 0.531576 tig0021157:-90515 None:intergenic
CCGTCCCGTTCGACTCATAC+AGG 0.533062 tig0021157:+88516 Msa1388710:CDS
CTCCGCACTGTGAACAATAG+TGG 0.533503 tig0021157:-88109 None:intergenic
CTCAGAAGTAAATTTCGGAT+GGG 0.534991 tig0021157:+91040 Msa1388710:CDS
TTCTTGGAAGCAAGGGAATG+GGG 0.535703 tig0021157:-88455 None:intergenic
TCTCAGAAGTAAATTTCGGA+TGG 0.535849 tig0021157:+91039 Msa1388710:CDS
AAAGAGCTAAATGATATGCT+TGG 0.536007 tig0021157:+91209 Msa1388710:CDS
TGAGACAATTCTTGACTGCA+TGG 0.536512 tig0021157:+90418 Msa1388710:CDS
TTGAGCTTGAACCATACAAT+GGG 0.537449 tig0021157:+90381 Msa1388710:CDS
ACCACTGCAAAGGAGCTTTG+TGG 0.545376 tig0021157:+90858 Msa1388710:CDS
CCGCACAAAAGCATTGCAGG+TGG 0.552363 tig0021157:+90719 Msa1388710:CDS
TTGTTTGAGTGATAACGCTA+TGG 0.553624 tig0021157:-88015 None:intergenic
ATGTGTGTGCAATAATGAAA+AGG 0.554289 tig0021157:+91357 Msa1388710:three_prime_UTR
ACACCGTTCACGTCTTGTGT+TGG 0.555223 tig0021157:-91644 None:intergenic
AATATCCGACAAGCAACAAA+GGG 0.562922 tig0021157:-90803 None:intergenic
GAGTTTAACTTCATGAGGAG+TGG 0.565045 tig0021157:-88164 None:intergenic
TGGAACCAGGCGACGAATCG+CGG 0.566895 tig0021157:-90640 None:intergenic
TCCACAAAGCTCCTTTGCAG+TGG 0.567433 tig0021157:-90859 None:intergenic
CATTGTATGGTTCAAGCTCA+AGG 0.569460 tig0021157:-90379 None:intergenic
AAGGTAGACAAGATGAAACT+AGG 0.571958 tig0021157:-91260 None:intergenic
ATAGAATTTCTTGGAAGCAA+GGG 0.573708 tig0021157:-88462 None:intergenic
GGCAGCAGTCACCACTGCAA+AGG 0.576150 tig0021157:+90848 Msa1388710:CDS
GTACAAACTAGAGACGATAC+AGG 0.576675 tig0021157:-87967 None:intergenic
TCAGAAGTAAATTTCGGATG+GGG 0.577426 tig0021157:+91041 Msa1388710:CDS
CAACAAAACAAAATTGTGCT+AGG 0.577670 tig0021157:+91676 Msa1388710:three_prime_UTR
GCAGCTGGAAGTACCAACTC+TGG 0.584510 tig0021157:-88139 None:intergenic
GACGTGAACGGTGTGTAACA+GGG 0.586024 tig0021157:+91653 Msa1388710:three_prime_UTR
CTTGAGCTTGAACCATACAA+TGG 0.589328 tig0021157:+90380 Msa1388710:CDS
AGCAAAAGCTCAAGTTACAG+AGG 0.589925 tig0021157:+90917 Msa1388710:CDS
ATAAATAAATATGAAAAGCA+AGG 0.590393 tig0021157:+91392 Msa1388710:three_prime_UTR
TAATATCCGACAAGCAACAA+AGG 0.591430 tig0021157:-90804 None:intergenic
GCAGGTGGAGCCAGATGAAG+AGG 0.592868 tig0021157:+90734 Msa1388710:CDS
TCTTGGAAGCAAGGGAATGG+GGG 0.595701 tig0021157:-88454 None:intergenic
GAGACAATTCTTGACTGCAT+GGG 0.598914 tig0021157:+90419 Msa1388710:CDS
AGTTGATGGTGGATTGCACT+GGG 0.599197 tig0021157:+88365 Msa1388710:CDS
GAGAGGAGTTTAACTTCATG+AGG 0.599961 tig0021157:-88169 None:intergenic
TGGGGTGAAGCTGTGTATGG+TGG 0.601746 tig0021157:+91059 Msa1388710:CDS
TGACGAAAGACAAATATGAC+AGG 0.607837 tig0021157:-88229 None:intergenic
GTCTCTAGTTTGTACGTACG+TGG 0.608491 tig0021157:+87975 Msa1388710:five_prime_UTR
ATTACTATCCAGCTGCAGGA+AGG 0.614273 tig0021157:+88320 Msa1388710:CDS
GCGGAGGTCCCAACCAGAGT+TGG 0.615833 tig0021157:+88126 Msa1388710:CDS
TGACAGGAAGATTGAAACGA+AGG 0.615959 tig0021157:-88213 None:intergenic
TAAGACCTGTATGAGTCGAA+CGG 0.619194 tig0021157:-88521 None:intergenic
GTGCATTGAGAAAGGTGAAG+TGG 0.620215 tig0021157:-90660 None:intergenic
TCCGCCAAATACGTACATCA+AGG 0.620796 tig0021157:+90560 Msa1388710:CDS
GCCTTCTTGATCATCTATGT+CGG 0.622425 tig0021157:-88191 None:intergenic
GTAACCAAAATCATCCTACA+AGG 0.623110 tig0021157:+91231 Msa1388710:CDS
TGGTTGCAAGTAACGTGTGG+CGG 0.626899 tig0021157:-90516 None:intergenic
TTGAACCATACAATGGGCGA+TGG 0.631288 tig0021157:+90387 Msa1388710:CDS
TGCTGGGCGAAAGTTGATGG+TGG 0.635555 tig0021157:+88354 Msa1388710:CDS
CCTGTATGAGTCGAACGGGA+CGG 0.638129 tig0021157:-88516 None:intergenic
GTGTGCAATAATGAAAAGGA+AGG 0.644525 tig0021157:+91361 Msa1388710:three_prime_UTR
TCCGACATAGATGATCAAGA+AGG 0.646509 tig0021157:+88190 Msa1388710:CDS
AAACCAACACAAGACGTGAA+CGG 0.648957 tig0021157:+91641 Msa1388710:three_prime_UTR
AAACCTCTTCATATCTTCAG+GGG 0.652653 tig0021157:-91183 None:intergenic
TATGGTGGAGCAGTAAAAGG+TGG 0.653295 tig0021157:+91074 Msa1388710:CDS
CATGTTTAAGAACCTTAACA+GGG 0.656931 tig0021157:-88276 None:intergenic
AGCTGCAGGAAGGATTAGGG+AGG 0.663235 tig0021157:+88330 Msa1388710:CDS
AGAATGCAAAAGGAGAAGAA+GGG 0.663405 tig0021157:+91138 Msa1388710:CDS
GTTACACGTCTCAAGTGTGG+TGG 0.666120 tig0021157:+90345 Msa1388710:CDS
AACCTCTTCATATCTTCAGG+GGG 0.666136 tig0021157:-91182 None:intergenic
CAGGTTACACGTCTCAAGTG+TGG 0.668116 tig0021157:+90342 Msa1388710:intron
CGATGGTTGCAAGTAACGTG+TGG 0.669479 tig0021157:-90519 None:intergenic
GTATTTGTTCGTATTCACGA+TGG 0.674672 tig0021157:-90536 None:intergenic
CTTGATCATCTATGTCGGAG+AGG 0.675142 tig0021157:-88186 None:intergenic
CACTATTGTTCACAGTGCGG+AGG 0.677341 tig0021157:+88110 Msa1388710:CDS
GCATGGGCAGAAATGGCTCG+AGG 0.681407 tig0021157:+90435 Msa1388710:CDS
GCTGCAGGAAGGATTAGGGA+GGG 0.683826 tig0021157:+88331 Msa1388710:CDS
CACCACTATTGTTCACAGTG+CGG 0.732056 tig0021157:+88107 Msa1388710:CDS
GTTGATGGTGGATTGCACTG+GGG 0.762765 tig0021157:+88366 Msa1388710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATATAGTTTTCTAAATTT+TGG - tig0021157:89232-89251 None:intergenic 10.0%
!! TGTAATTAATATAAATTCAT+AGG - tig0021157:89821-89840 None:intergenic 10.0%
!! TGATCAATAATTAAATTTCA+AGG - tig0021157:88565-88584 None:intergenic 15.0%
!!! AAACACATTATTTTTAATAG+CGG - tig0021157:88892-88911 None:intergenic 15.0%
!! TAAGTATAAAATGACATAAA+AGG + tig0021157:88939-88958 Msa1388710:intron 15.0%
!! AAAATTTGTCGAATAAAAAA+TGG - tig0021157:89097-89116 None:intergenic 15.0%
!!! TATATAATTTATTTGTTGTG+TGG - tig0021157:89586-89605 None:intergenic 15.0%
!! AAATAATTGCATTATATAAG+TGG - tig0021157:89762-89781 None:intergenic 15.0%
!! TGAATCATTTATATATCAAT+CGG - tig0021157:91308-91327 None:intergenic 15.0%
!! ATAAATAAATATGAAAAGCA+AGG + tig0021157:91392-91411 Msa1388710:three_prime_UTR 15.0%
!!! TTTGTGTCTTTTTTTTTTAA+TGG + tig0021157:91602-91621 Msa1388710:three_prime_UTR 15.0%
!!! TTGTGTCTTTTTTTTTTAAT+GGG + tig0021157:91603-91622 Msa1388710:three_prime_UTR 15.0%
!!! AAACATCCTTGAAATTTTAT+AGG + tig0021157:88590-88609 Msa1388710:intron 20.0%
!!! AACACATTATTTTTAATAGC+GGG - tig0021157:88891-88910 None:intergenic 20.0%
!! GCTATTAAAAATAATGTGTT+TGG + tig0021157:88891-88910 Msa1388710:intron 20.0%
!! TCTTATATGAATTAAAAGCA+AGG + tig0021157:89188-89207 Msa1388710:intron 20.0%
!! TATCCCAAAAAATAAAATGA+GGG - tig0021157:89980-89999 None:intergenic 20.0%
!! TTATCCCAAAAAATAAAATG+AGG - tig0021157:89981-90000 None:intergenic 20.0%
!!! TACCATTTTTCGTAAAAAAT+TGG - tig0021157:90007-90026 None:intergenic 20.0%
!! ACTTAAAAATAGTGTAAAGT+TGG - tig0021157:90035-90054 None:intergenic 20.0%
!! TAACATATACATACATCTTT+AGG + tig0021157:90290-90309 Msa1388710:intron 20.0%
!! GATAATTAGAATTAAATGAC+AGG + tig0021157:90323-90342 Msa1388710:intron 20.0%
!! GAAAGATGTTTATTTACAAA+GGG + tig0021157:90978-90997 Msa1388710:CDS 20.0%
! TCATGCTATCATAAAAAAGA+AGG - tig0021157:88078-88097 None:intergenic 25.0%
! GATATAACACTTGATCAATT+TGG + tig0021157:88418-88437 Msa1388710:CDS 25.0%
! TCAATAATATATTCTGATCC+TGG - tig0021157:88496-88515 None:intergenic 25.0%
! AAGAGACCTATAAAATTTCA+AGG - tig0021157:88599-88618 None:intergenic 25.0%
!! TGATCTCATTACAATCATTT+TGG - tig0021157:88663-88682 None:intergenic 25.0%
! AAAATGATTGTAATGAGATC+AGG + tig0021157:88662-88681 Msa1388710:intron 25.0%
! AAATGATTGTAATGAGATCA+GGG + tig0021157:88663-88682 Msa1388710:intron 25.0%
!! TATTCCAAGTCTAAAATTCA+GGG - tig0021157:88729-88748 None:intergenic 25.0%
!! TTATTCCAAGTCTAAAATTC+AGG - tig0021157:88730-88749 None:intergenic 25.0%
!! GAATTTTAGACTTGGAATAA+AGG + tig0021157:88730-88749 Msa1388710:intron 25.0%
!!! TTTTGGTGTTCACTTTTTTT+AGG - tig0021157:89215-89234 None:intergenic 25.0%
! TCGTTTGTATATATACACAT+AGG - tig0021157:89556-89575 None:intergenic 25.0%
!! ATTATCATGCATTAACTTGA+TGG - tig0021157:89925-89944 None:intergenic 25.0%
!!! TAATTTTCAAGCTTTCTAAC+CGG + tig0021157:89941-89960 Msa1388710:intron 25.0%
! ATCCCAAAAAATAAAATGAG+GGG - tig0021157:89979-89998 None:intergenic 25.0%
!!! AGCCAATTTTTTACGAAAAA+TGG + tig0021157:90002-90021 Msa1388710:intron 25.0%
!!! TATTAGTCTTTGAAGTTATC+GGG - tig0021157:90075-90094 None:intergenic 25.0%
!!! CTATTAGTCTTTGAAGTTAT+CGG - tig0021157:90076-90095 None:intergenic 25.0%
! TTTCACATTAAATATGCCTT+GGG - tig0021157:90136-90155 None:intergenic 25.0%
! ATTTCACATTAAATATGCCT+TGG - tig0021157:90137-90156 None:intergenic 25.0%
!!! TCAATGACATTAGTCTTTTT+AGG - tig0021157:90247-90266 None:intergenic 25.0%
! GGAAAGATGTTTATTTACAA+AGG + tig0021157:90977-90996 Msa1388710:CDS 25.0%
! AAGTTCTCAGAAGTAAATTT+CGG + tig0021157:91035-91054 Msa1388710:CDS 25.0%
!!! CAAGGTCTATTTTCTTATTT+GGG + tig0021157:91410-91429 Msa1388710:three_prime_UTR 25.0%
!!! AAGGTCTATTTTCTTATTTG+GGG + tig0021157:91411-91430 Msa1388710:three_prime_UTR 25.0%
! AACAAATGAGAAAAGATTAG+AGG - tig0021157:91461-91480 None:intergenic 25.0%
! GAATGAAAGAGAAAAAAGAA+AGG - tig0021157:91572-91591 None:intergenic 25.0%
!!! CTTTTTTCTCTTTCATTCTT+TGG + tig0021157:91574-91593 Msa1388710:three_prime_UTR 25.0%
!! ACAGGAATTTTGAAAAGTCA+TGG - tig0021157:87952-87971 None:intergenic 30.0%
CAACACTTCTTAACTCTATT+TGG + tig0021157:88042-88061 Msa1388710:five_prime_UTR 30.0%
GCTATCATAAAAAAGAAGGA+GGG - tig0021157:88074-88093 None:intergenic 30.0%
TGCTATCATAAAAAAGAAGG+AGG - tig0021157:88075-88094 None:intergenic 30.0%
CATGTTTAAGAACCTTAACA+GGG - tig0021157:88279-88298 None:intergenic 30.0%
ATAGAATTTCTTGGAAGCAA+GGG - tig0021157:88465-88484 None:intergenic 30.0%
TATAGAATTTCTTGGAAGCA+AGG - tig0021157:88466-88485 None:intergenic 30.0%
GAACATCGTATAGAATTTCT+TGG - tig0021157:88474-88493 None:intergenic 30.0%
! ATTCCAAGTCTAAAATTCAG+GGG - tig0021157:88728-88747 None:intergenic 30.0%
!!! ACATAGTTTTGTCTATCATG+AGG - tig0021157:88756-88775 None:intergenic 30.0%
!! AATAAACTGTGAGGTTTTAG+TGG + tig0021157:89269-89288 Msa1388710:intron 30.0%
! GAATTAGCTTATAAGCTTGT+TGG - tig0021157:89493-89512 None:intergenic 30.0%
ACTTTATGATGCTTACAACA+TGG + tig0021157:89667-89686 Msa1388710:intron 30.0%
CTTTATGATGCTTACAACAT+GGG + tig0021157:89668-89687 Msa1388710:intron 30.0%
CTAAAGCGAACTATAATATG+AGG - tig0021157:89858-89877 None:intergenic 30.0%
CTCATATTATAGTTCGCTTT+AGG + tig0021157:89856-89875 Msa1388710:intron 30.0%
!!! GACCCCTCATTTTATTTTTT+GGG + tig0021157:89974-89993 Msa1388710:intron 30.0%
!! TCAAAGACTAATAGCTTGTT+TGG + tig0021157:90082-90101 Msa1388710:intron 30.0%
!! TAATAGCTTGTTTGGAATAG+CGG + tig0021157:90090-90109 Msa1388710:intron 30.0%
ATCTTATCACTGCATGTTAT+TGG + tig0021157:90691-90710 Msa1388710:CDS 30.0%
TGGTAATTGTATTGCTTTCT+CGG + tig0021157:90827-90846 Msa1388710:CDS 30.0%
! GGCAGATTTTATGGTTATTA+AGG + tig0021157:90956-90975 Msa1388710:CDS 30.0%
AAAGAGCTAAATGATATGCT+TGG + tig0021157:91209-91228 Msa1388710:CDS 30.0%
ATCACAAGCTACAAACTATA+AGG - tig0021157:91282-91301 None:intergenic 30.0%
ATGTGTGTGCAATAATGAAA+AGG + tig0021157:91357-91376 Msa1388710:three_prime_UTR 30.0%
! GCAAGGTCTATTTTCTTATT+TGG + tig0021157:91409-91428 Msa1388710:three_prime_UTR 30.0%
!!! AGTTTGTTTTGTTGTGTCTT+TGG + tig0021157:91513-91532 Msa1388710:three_prime_UTR 30.0%
CAACAAAACAAAATTGTGCT+AGG + tig0021157:91676-91695 Msa1388710:three_prime_UTR 30.0%
!! GGAATTTTGAAAAGTCATGG+AGG - tig0021157:87949-87968 None:intergenic 35.0%
! TTGTTTGAGTGATAACGCTA+TGG - tig0021157:88018-88037 None:intergenic 35.0%
! AGTTTAACTTCATGAGGAGT+GGG - tig0021157:88166-88185 None:intergenic 35.0%
TGACGAAAGACAAATATGAC+AGG - tig0021157:88232-88251 None:intergenic 35.0%
GCATGTTTAAGAACCTTAAC+AGG - tig0021157:88280-88299 None:intergenic 35.0%
GAAATTCTATACGATGTTCC+AGG + tig0021157:88475-88494 Msa1388710:CDS 35.0%
GACAAAACTATGTCTCTGTT+TGG + tig0021157:88763-88782 Msa1388710:intron 35.0%
GGTAAAATTAGCTGTTGAAG+TGG + tig0021157:88912-88931 Msa1388710:intron 35.0%
ATATACACATAGGCTGTGTT+TGG - tig0021157:89546-89565 None:intergenic 35.0%
ACTTTGTCATGAAGCAAAGA+TGG + tig0021157:89634-89653 Msa1388710:intron 35.0%
GTGCATTGTAAAGTTGTATG+AGG - tig0021157:89704-89723 None:intergenic 35.0%
! AAGTGTTGTAAGTGTAGCAT+TGG - tig0021157:89739-89758 None:intergenic 35.0%
TTTAGGCCTTAGAATGTGTT+AGG + tig0021157:89873-89892 Msa1388710:intron 35.0%
!!! GGACCCCTCATTTTATTTTT+TGG + tig0021157:89973-89992 Msa1388710:intron 35.0%
TTGAGCTTGAACCATACAAT+GGG + tig0021157:90381-90400 Msa1388710:CDS 35.0%
CATTCAACCTGTATGGAATA+GGG + tig0021157:90473-90492 Msa1388710:CDS 35.0%
ATGGAATAGGGAGATTCTAA+TGG + tig0021157:90485-90504 Msa1388710:CDS 35.0%
GTATTTGTTCGTATTCACGA+TGG - tig0021157:90539-90558 None:intergenic 35.0%
TCTTCCTTGATGTACGTATT+TGG - tig0021157:90567-90586 None:intergenic 35.0%
CTGTGAAGAAGAAAGATTGA+TGG - tig0021157:90608-90627 None:intergenic 35.0%
AATATCCGACAAGCAACAAA+GGG - tig0021157:90806-90825 None:intergenic 35.0%
TAATATCCGACAAGCAACAA+AGG - tig0021157:90807-90826 None:intergenic 35.0%
! TTGTTGCTTGTCGGATATTA+TGG + tig0021157:90807-90826 Msa1388710:CDS 35.0%
ACTCAACTGCATAACCTAAT+GGG - tig0021157:90887-90906 None:intergenic 35.0%
AACTCAACTGCATAACCTAA+TGG - tig0021157:90888-90907 None:intergenic 35.0%
! GCATTCTTTGGCAGATTTTA+TGG + tig0021157:90947-90966 Msa1388710:CDS 35.0%
TACAAAGGGAAGAACTTGTA+TGG + tig0021157:90992-91011 Msa1388710:CDS 35.0%
TCTCAGAAGTAAATTTCGGA+TGG + tig0021157:91039-91058 Msa1388710:CDS 35.0%
CTCAGAAGTAAATTTCGGAT+GGG + tig0021157:91040-91059 Msa1388710:CDS 35.0%
TCAGAAGTAAATTTCGGATG+GGG + tig0021157:91041-91060 Msa1388710:CDS 35.0%
! TCTCCTTTTGCATTCTTATG+CGG - tig0021157:91134-91153 None:intergenic 35.0%
AAGAATGCAAAAGGAGAAGA+AGG + tig0021157:91137-91156 Msa1388710:CDS 35.0%
AGAATGCAAAAGGAGAAGAA+GGG + tig0021157:91138-91157 Msa1388710:CDS 35.0%
AAACCTCTTCATATCTTCAG+GGG - tig0021157:91186-91205 None:intergenic 35.0%
CAAACCTCTTCATATCTTCA+GGG - tig0021157:91187-91206 None:intergenic 35.0%
ACAAACCTCTTCATATCTTC+AGG - tig0021157:91188-91207 None:intergenic 35.0%
GTAACCAAAATCATCCTACA+AGG + tig0021157:91231-91250 Msa1388710:CDS 35.0%
AAGGTAGACAAGATGAAACT+AGG - tig0021157:91263-91282 None:intergenic 35.0%
GTGTGCAATAATGAAAAGGA+AGG + tig0021157:91361-91380 Msa1388710:three_prime_UTR 35.0%
!! CTATTTTCTTATTTGGGGTG+TGG + tig0021157:91416-91435 Msa1388710:three_prime_UTR 35.0%
!! TTTTGTTGTGTCTTTGGTCA+TGG + tig0021157:91519-91538 Msa1388710:three_prime_UTR 35.0%
GTACAAACTAGAGACGATAC+AGG - tig0021157:87970-87989 None:intergenic 40.0%
! GAGTTTAACTTCATGAGGAG+TGG - tig0021157:88167-88186 None:intergenic 40.0%
! GAGAGGAGTTTAACTTCATG+AGG - tig0021157:88172-88191 None:intergenic 40.0%
TCCGACATAGATGATCAAGA+AGG + tig0021157:88190-88209 Msa1388710:CDS 40.0%
GCCTTCTTGATCATCTATGT+CGG - tig0021157:88194-88213 None:intergenic 40.0%
TGACAGGAAGATTGAAACGA+AGG - tig0021157:88216-88235 None:intergenic 40.0%
GGGTCTTTCTCTTTCATTGA+TGG - tig0021157:88259-88278 None:intergenic 40.0%
GAAAGAGAAAGACCCTGTTA+AGG + tig0021157:88264-88283 Msa1388710:CDS 40.0%
GTTTATTACTATCCAGCTGC+AGG + tig0021157:88316-88335 Msa1388710:CDS 40.0%
TTTCTTGGAAGCAAGGGAAT+GGG - tig0021157:88459-88478 None:intergenic 40.0%
ATTTCTTGGAAGCAAGGGAA+TGG - tig0021157:88460-88479 None:intergenic 40.0%
TAAGACCTGTATGAGTCGAA+CGG - tig0021157:88524-88543 None:intergenic 40.0%
GATAGCTGATAGCTTATAGC+TGG + tig0021157:88861-88880 Msa1388710:intron 40.0%
GTTTATTGCAAGTCACGACA+TGG - tig0021157:89256-89275 None:intergenic 40.0%
! GTGACTTGCAATAAACTGTG+AGG + tig0021157:89260-89279 Msa1388710:intron 40.0%
GGATCAAAATGTGACGTGTT+TGG - tig0021157:89472-89491 None:intergenic 40.0%
! TGTTGTAAGTGTAGCATTGG+CGG - tig0021157:89736-89755 None:intergenic 40.0%
CTTTCCATCTCATTCAGTGT+CGG - tig0021157:89899-89918 None:intergenic 40.0%
CAAGTGTGGTGGTTTCATAA+TGG + tig0021157:90356-90375 Msa1388710:CDS 40.0%
CATTGTATGGTTCAAGCTCA+AGG - tig0021157:90382-90401 None:intergenic 40.0%
CTTGAGCTTGAACCATACAA+TGG + tig0021157:90380-90399 Msa1388710:CDS 40.0%
TGAGACAATTCTTGACTGCA+TGG + tig0021157:90418-90437 Msa1388710:CDS 40.0%
GAGACAATTCTTGACTGCAT+GGG + tig0021157:90419-90438 Msa1388710:CDS 40.0%
AACCTTCCATTCAACCTGTA+TGG + tig0021157:90466-90485 Msa1388710:CDS 40.0%
! TTCCATACAGGTTGAATGGA+AGG - tig0021157:90471-90490 None:intergenic 40.0%
! CCTATTCCATACAGGTTGAA+TGG - tig0021157:90475-90494 None:intergenic 40.0%
CCATTCAACCTGTATGGAAT+AGG + tig0021157:90472-90491 Msa1388710:CDS 40.0%
AGAATCTCCCTATTCCATAC+AGG - tig0021157:90483-90502 None:intergenic 40.0%
TCCTTGATGTACGTATTTGG+CGG - tig0021157:90564-90583 None:intergenic 40.0%
ATCATTCGTACCTCTTCATC+TGG - tig0021157:90747-90766 None:intergenic 40.0%
AGCAAAAGCTCAAGTTACAG+AGG + tig0021157:90917-90936 Msa1388710:CDS 40.0%
! AGAGGAGTACATGCATTCTT+TGG + tig0021157:90935-90954 Msa1388710:CDS 40.0%
! GAACTTGTATGGTGTCAGAT+TGG + tig0021157:91003-91022 Msa1388710:CDS 40.0%
! GTGGAGTTGGATCTTTTCTT+GGG + tig0021157:91093-91112 Msa1388710:CDS 40.0%
! TGGAGTTGGATCTTTTCTTG+GGG + tig0021157:91094-91113 Msa1388710:CDS 40.0%
ATTCTTATGCGGGACAAGAA+AGG - tig0021157:91123-91142 None:intergenic 40.0%
! CTCCTTTTGCATTCTTATGC+GGG - tig0021157:91133-91152 None:intergenic 40.0%
AACCTCTTCATATCTTCAGG+GGG - tig0021157:91185-91204 None:intergenic 40.0%
! CACTCCTTGTAGGATGATTT+TGG - tig0021157:91238-91257 None:intergenic 40.0%
! TTCTTATTTGGGGTGTGGAT+AGG + tig0021157:91421-91440 Msa1388710:three_prime_UTR 40.0%
! TCTTATTTGGGGTGTGGATA+GGG + tig0021157:91422-91441 Msa1388710:three_prime_UTR 40.0%
! TGTGTCTTTGGTCATGGTTT+TGG + tig0021157:91525-91544 Msa1388710:three_prime_UTR 40.0%
! GTGTCTTTGGTCATGGTTTT+GGG + tig0021157:91526-91545 Msa1388710:three_prime_UTR 40.0%
AAACCAACACAAGACGTGAA+CGG + tig0021157:91641-91660 Msa1388710:three_prime_UTR 40.0%
! GTCTCTAGTTTGTACGTACG+TGG + tig0021157:87975-87994 Msa1388710:five_prime_UTR 45.0%
CACCACTATTGTTCACAGTG+CGG + tig0021157:88107-88126 Msa1388710:CDS 45.0%
!! TGGAAGTACCAACTCTGGTT+GGG - tig0021157:88137-88156 None:intergenic 45.0%
CTTGATCATCTATGTCGGAG+AGG - tig0021157:88189-88208 None:intergenic 45.0%
ATTACTATCCAGCTGCAGGA+AGG + tig0021157:88320-88339 Msa1388710:CDS 45.0%
!! AAGTTGATGGTGGATTGCAC+TGG + tig0021157:88364-88383 Msa1388710:CDS 45.0%
!! AGTTGATGGTGGATTGCACT+GGG + tig0021157:88365-88384 Msa1388710:CDS 45.0%
TTCTTGGAAGCAAGGGAATG+GGG - tig0021157:88458-88477 None:intergenic 45.0%
AAGACCTGTATGAGTCGAAC+GGG - tig0021157:88523-88542 None:intergenic 45.0%
GATTGTAATGAGATCAGGGC+TGG + tig0021157:88667-88686 Msa1388710:intron 45.0%
! AGGCCCCTGAATTTTAGACT+TGG + tig0021157:88722-88741 Msa1388710:intron 45.0%
TGGCAGTAAGCCTTAAAACG+TGG + tig0021157:89289-89308 Msa1388710:intron 45.0%
GGCAGTAAGCCTTAAAACGT+GGG + tig0021157:89290-89309 Msa1388710:intron 45.0%
AGCCTTAAAACGTGGGAACA+TGG + tig0021157:89297-89316 Msa1388710:intron 45.0%
GCCTTAAAACGTGGGAACAT+GGG + tig0021157:89298-89317 Msa1388710:intron 45.0%
! ACCCATGTTCCCACGTTTTA+AGG - tig0021157:89302-89321 None:intergenic 45.0%
TTAAAACGTGGGAACATGGG+TGG + tig0021157:89301-89320 Msa1388710:intron 45.0%
TGTCGGCCTAACACATTCTA+AGG - tig0021157:89882-89901 None:intergenic 45.0%
AAGCTTTCTAACCGGTCCAA+CGG + tig0021157:89949-89968 Msa1388710:intron 45.0%
!! TTTGCATTTCTAGCGTCCCA+AGG + tig0021157:90117-90136 Msa1388710:intron 45.0%
CATTCACCGCTAAAACAGAC+AGG + tig0021157:90206-90225 Msa1388710:intron 45.0%
!! GTAGCTCCTGTCTGTTTTAG+CGG - tig0021157:90215-90234 None:intergenic 45.0%
TTGAACCATACAATGGGCGA+TGG + tig0021157:90387-90406 Msa1388710:CDS 45.0%
TCCGCCAAATACGTACATCA+AGG + tig0021157:90560-90579 Msa1388710:CDS 45.0%
GTGCATTGAGAAAGGTGAAG+TGG - tig0021157:90663-90682 None:intergenic 45.0%
CGTATGCAGTGCATTGAGAA+AGG - tig0021157:90671-90690 None:intergenic 45.0%
! GCTAACCCTTTGTTGCTTGT+CGG + tig0021157:90798-90817 Msa1388710:CDS 45.0%
! GAGCTTTGTGGAAACCCATT+AGG + tig0021157:90870-90889 Msa1388710:CDS 45.0%
! GTGTATGGTGGAGCAGTAAA+AGG + tig0021157:91071-91090 Msa1388710:CDS 45.0%
! TATGGTGGAGCAGTAAAAGG+TGG + tig0021157:91074-91093 Msa1388710:CDS 45.0%
! GGTGGAGTTGGATCTTTTCT+TGG + tig0021157:91092-91111 Msa1388710:CDS 45.0%
! GGAGTTGGATCTTTTCTTGG+GGG + tig0021157:91095-91114 Msa1388710:CDS 45.0%
GTCCCGCATAAGAATGCAAA+AGG + tig0021157:91128-91147 Msa1388710:CDS 45.0%
!! AAACTAGGAGCACTCCTTGT+AGG - tig0021157:91248-91267 None:intergenic 45.0%
!! ATAATAAAATTAAATTAACA+AGG + tig0021157:89344-89363 Msa1388710:intron 5.0%
CTCCGCACTGTGAACAATAG+TGG - tig0021157:88112-88131 None:intergenic 50.0%
CACTATTGTTCACAGTGCGG+AGG + tig0021157:88110-88129 Msa1388710:CDS 50.0%
!! CTGGAAGTACCAACTCTGGT+TGG - tig0021157:88138-88157 None:intergenic 50.0%
ATCCAGCTGCAGGAAGGATT+AGG + tig0021157:88326-88345 Msa1388710:CDS 50.0%
TCCAGCTGCAGGAAGGATTA+GGG + tig0021157:88327-88346 Msa1388710:CDS 50.0%
!! GTTGATGGTGGATTGCACTG+GGG + tig0021157:88366-88385 Msa1388710:CDS 50.0%
TCTTGGAAGCAAGGGAATGG+GGG - tig0021157:88457-88476 None:intergenic 50.0%
!! GTTTGGGTTGCCTTACCCTT+AGG - tig0021157:88693-88712 None:intergenic 50.0%
AATTCAGGGGCCTAAAGCGA+GGG - tig0021157:88715-88734 None:intergenic 50.0%
AAATTCAGGGGCCTAAAGCG+AGG - tig0021157:88716-88735 None:intergenic 50.0%
TAGGCCGACACTGAATGAGA+TGG + tig0021157:89892-89911 Msa1388710:intron 50.0%
TAAAATGAGGGGTCCACCGT+TGG - tig0021157:89968-89987 None:intergenic 50.0%
CAGGTTACACGTCTCAAGTG+TGG + tig0021157:90342-90361 Msa1388710:intron 50.0%
GTTACACGTCTCAAGTGTGG+TGG + tig0021157:90345-90364 Msa1388710:CDS 50.0%
CTTGACTGCATGGGCAGAAA+TGG + tig0021157:90428-90447 Msa1388710:CDS 50.0%
TGGTTGCAAGTAACGTGTGG+CGG - tig0021157:90519-90538 None:intergenic 50.0%
CGATGGTTGCAAGTAACGTG+TGG - tig0021157:90522-90541 None:intergenic 50.0%
GAGAAAGGTGAAGTGGAACC+AGG - tig0021157:90656-90675 None:intergenic 50.0%
! CCACCTGCAATGCTTTTGTG+CGG - tig0021157:90722-90741 None:intergenic 50.0%
GCATCGTTAATGCACGTTCG+AGG + tig0021157:90769-90788 Msa1388710:CDS 50.0%
ACCACTGCAAAGGAGCTTTG+TGG + tig0021157:90858-90877 Msa1388710:CDS 50.0%
TCCACAAAGCTCCTTTGCAG+TGG - tig0021157:90862-90881 None:intergenic 50.0%
GGAGCAGTAAAAGGTGGAGT+TGG + tig0021157:91080-91099 Msa1388710:CDS 50.0%
TCTTCAGGGGGCAAGCAAAT+TGG - tig0021157:91173-91192 None:intergenic 50.0%
TGCCCCCTGAAGATATGAAG+AGG + tig0021157:91180-91199 Msa1388710:CDS 50.0%
ACACCGTTCACGTCTTGTGT+TGG - tig0021157:91647-91666 None:intergenic 50.0%
AGACGTGAACGGTGTGTAAC+AGG + tig0021157:91652-91671 Msa1388710:three_prime_UTR 50.0%
GACGTGAACGGTGTGTAACA+GGG + tig0021157:91653-91672 Msa1388710:three_prime_UTR 50.0%
!! GCAGCTGGAAGTACCAACTC+TGG - tig0021157:88142-88161 None:intergenic 55.0%
TCCCTAATCCTTCCTGCAGC+TGG - tig0021157:88331-88350 None:intergenic 55.0%
AGCTGCAGGAAGGATTAGGG+AGG + tig0021157:88330-88349 Msa1388710:CDS 55.0%
GCTGCAGGAAGGATTAGGGA+GGG + tig0021157:88331-88350 Msa1388710:CDS 55.0%
!! GAAGGATTAGGGAGGGTGCT+GGG + tig0021157:88338-88357 Msa1388710:CDS 55.0%
!! TGCTGGGCGAAAGTTGATGG+TGG + tig0021157:88354-88373 Msa1388710:CDS 55.0%
!! ATGGTGGATTGCACTGGGGA+GGG + tig0021157:88370-88389 Msa1388710:CDS 55.0%
CCTGTATGAGTCGAACGGGA+CGG - tig0021157:88519-88538 None:intergenic 55.0%
!! ATGAGATCAGGGCTGGCCTA+AGG + tig0021157:88674-88693 Msa1388710:intron 55.0%
!! TGAGATCAGGGCTGGCCTAA+GGG + tig0021157:88675-88694 Msa1388710:intron 55.0%
GCAACCCAAACCCTCGCTTT+AGG + tig0021157:88702-88721 Msa1388710:intron 55.0%
CTTTCTAACCGGTCCAACGG+TGG + tig0021157:89952-89971 Msa1388710:intron 55.0%
CAGCTCCATCGCCCATTGTA+TGG - tig0021157:90395-90414 None:intergenic 55.0%
CATACAATGGGCGATGGAGC+TGG + tig0021157:90393-90412 Msa1388710:CDS 55.0%
GGTTGCAAGTAACGTGTGGC+GGG - tig0021157:90518-90537 None:intergenic 55.0%
!! CTGCCGCACAAAAGCATTGC+AGG + tig0021157:90716-90735 Msa1388710:CDS 55.0%
!! CCGCACAAAAGCATTGCAGG+TGG + tig0021157:90719-90738 Msa1388710:CDS 55.0%
GGATGGGGTGAAGCTGTGTA+TGG + tig0021157:91056-91075 Msa1388710:CDS 55.0%
TGGGGTGAAGCTGTGTATGG+TGG + tig0021157:91059-91078 Msa1388710:CDS 55.0%
TGGGGTGTGGATAGGGTTGA+TGG + tig0021157:91429-91448 Msa1388710:three_prime_UTR 55.0%
! GGGGTGTGGATAGGGTTGAT+GGG + tig0021157:91430-91449 Msa1388710:three_prime_UTR 55.0%
!! GGGTGTGGATAGGGTTGATG+GGG + tig0021157:91431-91450 Msa1388710:three_prime_UTR 55.0%
!! GGTGTGGATAGGGTTGATGG+GGG + tig0021157:91432-91451 Msa1388710:three_prime_UTR 55.0%
TCATGAGGAGTGGGTGCAGC+TGG - tig0021157:88157-88176 None:intergenic 60.0%
! GGAAGGATTAGGGAGGGTGC+TGG + tig0021157:88337-88356 Msa1388710:CDS 60.0%
!! GGGTGCTGGGCGAAAGTTGA+TGG + tig0021157:88351-88370 Msa1388710:CDS 60.0%
!! GATGGTGGATTGCACTGGGG+AGG + tig0021157:88369-88388 Msa1388710:CDS 60.0%
CCGTCCCGTTCGACTCATAC+AGG + tig0021157:88516-88535 Msa1388710:CDS 60.0%
!! TCAGGGCTGGCCTAAGGGTA+AGG + tig0021157:88680-88699 Msa1388710:intron 60.0%
GGGGCCTAAAGCGAGGGTTT+GGG - tig0021157:88709-88728 None:intergenic 60.0%
AGGGGCCTAAAGCGAGGGTT+TGG - tig0021157:88710-88729 None:intergenic 60.0%
GCATGGGCAGAAATGGCTCG+AGG + tig0021157:90435-90454 Msa1388710:CDS 60.0%
TGGAACCAGGCGACGAATCG+CGG - tig0021157:90643-90662 None:intergenic 60.0%
GCAGGTGGAGCCAGATGAAG+AGG + tig0021157:90734-90753 Msa1388710:CDS 60.0%
GGCAGCAGTCACCACTGCAA+AGG + tig0021157:90848-90867 Msa1388710:CDS 60.0%
GCGGAGGTCCCAACCAGAGT+TGG + tig0021157:88126-88145 Msa1388710:CDS 65.0%
TGAGGGGTCCACCGTTGGAC+CGG - tig0021157:89963-89982 None:intergenic 65.0%
CATAGCCGCGATTCGTCGCC+TGG + tig0021157:90635-90654 Msa1388710:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0021157 gene 87923 91711 87923 ID=Msa1388710;Name=Msa1388710
tig0021157 mRNA 87923 91711 87923 ID=Msa1388710-mRNA-1;Parent=Msa1388710;Name=Msa1388710-mRNA-1;_AED=0.01;_eAED=0.01;_QI=171|1|1|1|1|1|2|425|461
tig0021157 exon 87923 88537 87923 ID=Msa1388710-mRNA-1:exon:11232;Parent=Msa1388710-mRNA-1
tig0021157 exon 90345 91711 90345 ID=Msa1388710-mRNA-1:exon:11233;Parent=Msa1388710-mRNA-1
tig0021157 five_prime_UTR 87923 88093 87923 ID=Msa1388710-mRNA-1:five_prime_utr;Parent=Msa1388710-mRNA-1
tig0021157 CDS 88094 88537 88094 ID=Msa1388710-mRNA-1:cds;Parent=Msa1388710-mRNA-1
tig0021157 CDS 90345 91286 90345 ID=Msa1388710-mRNA-1:cds;Parent=Msa1388710-mRNA-1
tig0021157 three_prime_UTR 91287 91711 91287 ID=Msa1388710-mRNA-1:three_prime_utr;Parent=Msa1388710-mRNA-1
Gene Sequence

>Msa1388710

ATGACTTCTTCATCACCACTATTGTTCACAGTGCGGAGGTCCCAACCAGAGTTGGTACTTCCAGCTGCACCCACTCCTCATGAAGTTAAACTCCTCTCCGACATAGATGATCAAGAAGGCCTTCGTTTCAATCTTCCTGTCATATTTGTCTTTCGTCATGAACCATCAATGAAAGAGAAAGACCCTGTTAAGGTTCTTAAACATGCACTCTCACAAACACTTGTTTATTACTATCCAGCTGCAGGAAGGATTAGGGAGGGTGCTGGGCGAAAGTTGATGGTGGATTGCACTGGGGAGGGTGTCATGTTCATTGAAGCTGAAGCTGATATAACACTTGATCAATTTGGTGATGCTCTGCAACCCCCATTCCCTTGCTTCCAAGAAATTCTATACGATGTTCCAGGATCAGAATATATTATTGACCGTCCCGTTCGACTCATACAGGTTACACGTCTCAAGTGTGGTGGTTTCATAATGGCCTTGAGCTTGAACCATACAATGGGCGATGGAGCTGGTTTGAGACAATTCTTGACTGCATGGGCAGAAATGGCTCGAGGAGCAAATCAACCTTCCATTCAACCTGTATGGAATAGGGAGATTCTAATGGCAAGAGACCCGCCACACGTTACTTGCAACCATCGTGAATACGAACAAATACTTCCGCCAAATACGTACATCAAGGAAGAAGATACTACTATTGTAGTCCATCAATCTTTCTTCTTCACAGCTGCTCACATAGCCGCGATTCGTCGCCTGGTTCCACTTCACCTTTCTCAATGCACTGCATACGATCTTATCACTGCATGTTATTGGTGCTGCCGCACAAAAGCATTGCAGGTGGAGCCAGATGAAGAGGTACGAATGATGTGCATCGTTAATGCACGTTCGAGGTTTAGTGCTAACCCTTTGTTGCTTGTCGGATATTATGGTAATTGTATTGCTTTCTCGGCAGCAGTCACCACTGCAAAGGAGCTTTGTGGAAACCCATTAGGTTATGCAGTTGAGTTGATAAGAAAAGCAAAAGCTCAAGTTACAGAGGAGTACATGCATTCTTTGGCAGATTTTATGGTTATTAAGGAAAGATGTTTATTTACAAAGGGAAGAACTTGTATGGTGTCAGATTGGACTCGTGCTAAGTTCTCAGAAGTAAATTTCGGATGGGGTGAAGCTGTGTATGGTGGAGCAGTAAAAGGTGGAGTTGGATCTTTTCTTGGGGGAACCTTTCTTGTCCCGCATAAGAATGCAAAAGGAGAAGAAGGGTTAATATTACCAATTTGCTTGCCCCCTGAAGATATGAAGAGGTTTGTTAAAGAGCTAAATGATATGCTTGGTAACCAAAATCATCCTACAAGGAGTGCTCCTAGTTTCATCTTGTCTACCTTATAG
Protein sequence

>Msa1388710

MTSSSPLLFTVRRSQPELVLPAAPTPHEVKLLSDIDDQEGLRFNLPVIFVFRHEPSMKEKDPVKVLKHALSQTLVYYYPAAGRIREGAGRKLMVDCTGEGVMFIEAEADITLDQFGDALQPPFPCFQEILYDVPGSEYIIDRPVRLIQVTRLKCGGFIMALSLNHTMGDGAGLRQFLTAWAEMARGANQPSIQPVWNREILMARDPPHVTCNHREYEQILPPNTYIKEEDTTIVVHQSFFFTAAHIAAIRRLVPLHLSQCTAYDLITACYWCCRTKALQVEPDEEVRMMCIVNARSRFSANPLLLVGYYGNCIAFSAAVTTAKELCGNPLGYAVELIRKAKAQVTEEYMHSLADFMVIKERCLFTKGRTCMVSDWTRAKFSEVNFGWGEAVYGGAVKGGVGSFLGGTFLVPHKNAKGEEGLILPICLPPEDMKRFVKELNDMLGNQNHPTRSAPSFILSTL*