AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1432750

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1432750 MtrunA17_Chr7g0273721 94.372 462 25 1 1 461 1 462 0.0 914
Msa1432750 MtrunA17_Chr5g0433641 87.146 459 59 0 3 461 2 460 0.0 852
Msa1432750 MtrunA17_Chr7g0273791 77.851 456 100 1 3 458 2 456 0.0 760
Msa1432750 MtrunA17_Chr7g0273781 76.790 461 105 2 1 461 34 492 0.0 756
Msa1432750 MtrunA17_Chr7g0273761 75.541 462 112 1 1 461 372 833 0.0 740
Msa1432750 MtrunA17_Chr7g0273761 71.197 309 89 0 148 456 32 340 8.92e-157 465
Msa1432750 MtrunA17_Chr7g0273731 75.839 447 107 1 1 447 83 528 0.0 731
Msa1432750 MtrunA17_Chr1g0165671 60.131 459 175 5 7 461 23 477 0.0 572
Msa1432750 MtrunA17_Chr1g0165681 53.896 462 201 8 7 461 5 461 7.46e-178 506
Msa1432750 MtrunA17_Chr1g0165661 54.248 459 201 6 7 461 5 458 3.98e-177 504
Msa1432750 MtrunA17_Chr1g0191081 56.858 452 180 7 3 447 9 452 1.61e-175 499
Msa1432750 MtrunA17_Chr1g0165711 51.092 458 176 7 7 461 5 417 1.94e-153 442
Msa1432750 MtrunA17_Chr7g0273701 89.655 174 18 0 288 461 1 174 1.01e-114 334
Msa1432750 MtrunA17_Chr7g0273741 81.633 147 27 0 3 149 2 148 5.23e-83 252
Msa1432750 MtrunA17_Chr6g0471381 51.765 255 117 5 199 450 6 257 6.80e-83 256
Msa1432750 MtrunA17_Chr8g0386141 35.733 389 236 8 9 392 5 384 7.29e-81 257
Msa1432750 MtrunA17_Chr3g0132001 33.411 428 269 8 11 435 14 428 1.69e-79 253
Msa1432750 MtrunA17_Chr7g0224391 35.052 388 238 8 14 396 19 397 1.81e-76 245
Msa1432750 MtrunA17_Chr3g0098961 72.483 149 41 0 1 149 3 151 2.05e-73 229
Msa1432750 MtrunA17_Chr6g0471371 60.625 160 54 1 7 166 5 155 9.47e-66 209
Msa1432750 MtrunA17_Chr1g0165741 63.636 143 52 0 7 149 5 147 1.51e-62 199
Msa1432750 MtrunA17_Chr6g0471971 45.714 245 106 6 163 404 1 221 6.25e-61 198
Msa1432750 MtrunA17_Chr2g0306321 30.505 436 284 12 19 439 26 457 2.43e-59 201
Msa1432750 MtrunA17_Chr8g0353421 63.780 127 46 0 4 130 2 128 2.80e-57 185
Msa1432750 MtrunA17_Chr7g0273691 87.368 95 12 0 167 261 1 95 4.25e-56 181
Msa1432750 MtrunA17_Chr3g0141851 31.378 392 243 11 21 400 22 399 5.09e-56 192
Msa1432750 MtrunA17_Chr2g0334001 29.464 448 288 14 7 442 1 432 2.10e-55 190
Msa1432750 MtrunA17_Chr4g0027071 30.493 446 274 16 18 439 19 452 3.47e-52 182
Msa1432750 MtrunA17_Chr4g0001861 29.356 419 258 14 43 442 34 433 7.77e-51 178
Msa1432750 MtrunA17_Chr8g0371821 27.088 443 295 13 7 439 1 425 7.25e-46 164
Msa1432750 MtrunA17_Chr3g0113821 29.500 400 262 10 10 396 4 396 3.48e-45 163
Msa1432750 MtrunA17_Chr2g0283361 31.778 343 221 7 56 391 48 384 9.91e-44 159
Msa1432750 MtrunA17_Chr7g0270771 31.000 300 164 8 7 293 1 270 3.35e-42 150
Msa1432750 MtrunA17_Chr7g0253501 27.952 415 253 13 18 394 9 415 9.69e-39 146
Msa1432750 MtrunA17_Chr7g0253531 28.434 415 253 15 17 394 8 415 1.30e-38 145
Msa1432750 MtrunA17_Chr6g0471891 42.408 191 82 4 234 422 1 165 1.45e-38 139
Msa1432750 MtrunA17_Chr4g0072041 27.938 451 279 14 19 439 22 456 2.33e-38 145
Msa1432750 MtrunA17_Chr7g0252731 28.082 438 274 16 4 429 8 416 7.20e-38 143
Msa1432750 MtrunA17_Chr3g0087401 25.747 435 258 12 19 415 10 417 2.32e-37 142
Msa1432750 MtrunA17_Chr4g0054081 27.294 436 267 14 37 439 34 452 1.52e-36 140
Msa1432750 MtrunA17_Chr2g0311401 29.545 308 195 10 147 442 5 302 1.84e-36 136
Msa1432750 MtrunA17_Chr7g0253541 24.894 470 287 18 19 443 12 460 1.40e-34 134
Msa1432750 MtrunA17_Chr6g0470591 27.723 404 249 15 65 439 70 459 8.21e-33 129
Msa1432750 MtrunA17_Chr3g0106691 26.786 392 263 11 18 391 63 448 1.56e-32 129
Msa1432750 MtrunA17_Chr3g0106661 27.983 461 277 18 18 439 17 461 1.77e-30 123
Msa1432750 MtrunA17_Chr5g0442691 25.891 421 262 14 9 393 31 437 9.21e-30 121
Msa1432750 MtrunA17_Chr1g0165721 50.909 110 52 2 349 456 1 110 4.59e-29 110
Msa1432750 MtrunA17_Chr5g0442701 25.891 421 262 14 9 393 8 414 5.13e-29 119
Msa1432750 MtrunA17_Chr7g0235531 25.000 444 288 14 18 437 11 433 7.57e-29 118
Msa1432750 MtrunA17_Chr3g0106721 29.730 296 183 9 61 341 63 348 1.17e-27 114
Msa1432750 MtrunA17_Chr7g0273681 86.538 52 7 0 93 144 1 52 1.14e-26 101
Msa1432750 MtrunA17_Chr3g0106711 33.508 191 115 5 18 199 17 204 5.01e-26 105
Msa1432750 MtrunA17_Chr4g0004691 33.721 172 100 5 19 186 12 173 1.02e-25 103
Msa1432750 MtrunA17_Chr1g0165731 43.860 114 57 4 163 273 1 110 1.27e-25 100
Msa1432750 MtrunA17_Chr5g0442721 26.277 411 252 16 63 438 64 458 2.97e-25 108
Msa1432750 MtrunA17_Chr3g0113181 24.299 428 256 12 54 435 54 459 1.08e-24 106
Msa1432750 MtrunA17_Chr3g0134091 26.097 433 269 16 25 432 30 436 3.45e-24 104
Msa1432750 MtrunA17_Chr5g0408911 24.826 431 274 15 24 430 33 437 4.73e-24 104
Msa1432750 MtrunA17_Chr3g0134121 26.526 426 275 17 26 432 31 437 7.25e-24 103
Msa1432750 MtrunA17_Chr3g0106761 25.915 328 217 10 81 391 29 347 8.45e-24 103
Msa1432750 MtrunA17_Chr3g0106741 26.300 327 217 9 81 391 60 378 9.14e-23 100
Msa1432750 MtrunA17_Chr3g0134041 25.990 404 247 16 66 442 66 444 1.15e-22 100
Msa1432750 MtrunA17_Chr3g0095731 23.404 423 253 14 44 428 43 432 6.06e-22 98.2
Msa1432750 MtrunA17_Chr8g0354371 24.812 399 263 13 25 405 31 410 2.55e-21 96.3
Msa1432750 MtrunA17_Chr3g0123061 23.756 442 274 19 17 439 11 408 2.94e-21 95.5
Msa1432750 MtrunA17_Chr3g0125001 24.594 431 258 19 25 428 25 415 4.48e-21 95.1
Msa1432750 MtrunA17_Chr3g0095721 23.192 401 240 13 63 428 69 436 1.03e-20 94.4
Msa1432750 MtrunA17_Chr3g0134111 24.885 434 271 16 26 432 32 437 1.29e-20 94.0
Msa1432750 MtrunA17_Chr3g0134071 25.558 403 243 18 38 405 44 424 1.72e-20 94.0
Msa1432750 MtrunA17_Chr5g0438701 24.201 438 258 21 17 422 9 404 3.60e-20 92.8
Msa1432750 MtrunA17_Chr4g0010381 24.480 433 263 19 25 428 25 422 2.18e-19 90.1
Msa1432750 MtrunA17_Chr8g0374641 23.953 430 269 19 25 428 30 427 7.92e-19 88.6
Msa1432750 MtrunA17_Chr3g0134061 24.299 428 268 17 38 432 42 446 1.00e-18 88.6
Msa1432750 MtrunA17_Chr4g0053111 24.582 419 249 16 25 405 28 417 1.09e-18 88.2
Msa1432750 MtrunA17_Chr4g0010331 23.218 435 271 16 25 433 25 422 9.11e-18 85.5
Msa1432750 MtrunA17_Chr7g0273671 88.636 44 4 1 1 44 1 43 2.03e-17 75.9
Msa1432750 MtrunA17_Chr1g0199561 24.221 417 255 16 17 399 9 398 3.25e-17 83.6
Msa1432750 MtrunA17_Chr8g0374911 23.776 429 274 19 26 428 52 453 1.08e-16 82.4
Msa1432750 MtrunA17_Chr6g0471981 60.784 51 20 0 102 152 1 51 2.92e-16 73.2
Msa1432750 MtrunA17_Chr4g0053081 22.573 412 261 17 25 406 33 416 2.60e-15 77.8
Msa1432750 MtrunA17_Chr8g0361771 23.716 409 262 19 17 399 9 393 3.05e-15 77.8
Msa1432750 MtrunA17_Chr8g0359251 22.406 424 284 15 25 430 30 426 1.37e-14 75.5
Msa1432750 MtrunA17_Chr1g0169471 37.374 99 54 2 91 188 13 104 5.24e-14 68.6
Msa1432750 MtrunA17_Chr3g0087901 22.222 432 273 15 46 435 46 456 1.24e-13 72.8
Msa1432750 MtrunA17_Chr5g0414431 55.357 56 20 1 53 108 2 52 1.36e-13 65.1
Msa1432750 MtrunA17_Chr7g0252771 25.490 204 142 5 194 392 7 205 2.84e-13 69.7
Msa1432750 MtrunA17_Chr6g0456591 28.846 156 100 6 61 208 64 216 7.34e-13 68.2
Msa1432750 MtrunA17_Chr3g0106631 26.761 142 96 4 149 285 2 140 2.15e-11 62.4
Msa1432750 MtrunA17_Chr2g0311411 29.730 148 98 4 7 154 1 142 7.86e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1432750 AT5G17540.1 48.387 465 228 8 4 461 2 461 1.04e-148 432
Msa1432750 AT3G03480.1 47.427 447 225 7 2 444 10 450 4.32e-138 404
Msa1432750 AT3G48720.1 32.581 399 257 8 1 396 1 390 1.35e-75 243
Msa1432750 AT5G41040.1 35.190 395 236 8 11 396 33 416 1.42e-75 244
Msa1432750 AT5G41040.2 35.190 395 236 8 11 396 17 400 1.50e-75 243
Msa1432750 AT5G63560.1 35.533 394 230 9 11 396 9 386 1.80e-74 240
Msa1432750 AT1G27620.1 30.070 429 274 10 14 435 12 421 1.26e-58 199
Msa1432750 AT1G03390.1 29.978 447 289 14 9 441 23 459 8.68e-56 192
Msa1432750 AT5G48930.1 28.670 436 282 13 18 439 10 430 7.07e-49 173
Msa1432750 AT2G19070.1 29.630 459 278 14 10 439 5 447 1.14e-46 167
Msa1432750 AT3G62160.1 29.703 404 248 11 7 398 1 380 4.04e-46 166
Msa1432750 AT2G40230.1 29.250 400 257 12 11 396 5 392 3.78e-44 160
Msa1432750 AT5G57840.1 27.609 460 284 16 11 444 3 439 2.66e-42 155
Msa1432750 AT1G28680.1 30.189 371 238 12 32 391 27 387 1.12e-41 154
Msa1432750 AT5G07080.1 29.854 412 254 13 1 391 6 403 2.26e-41 153
Msa1432750 AT1G65450.1 27.412 456 301 15 4 443 8 449 1.18e-40 151
Msa1432750 AT3G47170.1 27.521 476 285 17 9 443 10 466 3.74e-39 147
Msa1432750 AT1G28680.2 29.891 368 230 13 32 381 27 384 5.12e-38 143
Msa1432750 AT2G25150.1 28.330 473 276 17 6 442 7 452 2.38e-35 137
Msa1432750 AT2G23510.1 28.916 415 246 17 3 391 7 398 2.42e-34 134
Msa1432750 AT1G31490.1 23.775 408 265 14 8 393 3 386 5.71e-26 110
Msa1432750 AT4G31910.1 26.226 469 300 20 6 441 2 457 1.43e-25 109
Msa1432750 AT5G42830.1 27.982 436 265 19 21 433 27 436 1.82e-25 108
Msa1432750 AT4G15390.1 25.225 444 278 20 17 435 14 428 1.98e-25 108
Msa1432750 AT4G15390.2 25.225 444 278 20 17 435 14 428 1.98e-25 108
Msa1432750 AT5G07860.1 25.570 395 243 15 63 433 69 436 6.96e-23 101
Msa1432750 AT3G50280.1 25.862 406 255 16 25 409 28 408 2.06e-22 99.8
Msa1432750 AT3G50270.1 25.121 414 267 13 25 417 30 421 3.86e-22 99.0
Msa1432750 AT3G26040.1 23.457 405 253 15 17 394 9 383 7.38e-22 98.2
Msa1432750 AT1G24430.1 25.629 437 274 18 17 435 11 414 8.35e-22 97.8
Msa1432750 AT1G78990.1 24.528 371 244 11 44 394 45 399 4.62e-21 95.9
Msa1432750 AT5G38130.1 25.679 405 257 15 25 410 48 427 4.69e-21 95.9
Msa1432750 AT5G07850.1 24.276 449 268 19 21 433 28 440 1.10e-20 94.7
Msa1432750 AT1G24420.2 25.390 449 264 18 17 435 11 418 1.28e-20 94.4
Msa1432750 AT1G24420.1 25.390 449 264 18 17 435 11 418 1.28e-20 94.4
Msa1432750 AT3G30280.1 24.208 442 278 19 17 432 12 422 1.08e-19 91.7
Msa1432750 AT1G32910.1 23.077 390 249 12 64 431 63 423 1.64e-19 91.3
Msa1432750 AT5G47980.1 25.670 448 277 18 15 436 9 426 2.55e-19 90.5
Msa1432750 AT1G65450.3 25.676 296 198 10 159 443 1 285 2.97e-19 88.2
Msa1432750 AT2G39980.1 23.628 419 254 12 66 432 62 466 7.07e-19 89.4
Msa1432750 AT5G23970.1 25.901 444 262 19 17 435 9 410 4.36e-18 86.7
Msa1432750 AT5G47950.1 23.678 435 272 19 17 432 12 405 1.22e-17 85.1
Msa1432750 AT5G16410.1 23.410 346 234 12 66 394 91 422 2.46e-17 84.7
Msa1432750 AT3G50300.1 23.784 370 244 12 63 410 56 409 9.03e-16 79.7
Msa1432750 AT4G15400.1 26.577 444 270 20 17 438 11 420 2.08e-15 78.6
Msa1432750 AT5G23940.1 24.331 411 265 16 53 437 53 443 1.66e-14 75.9
Msa1432750 AT3G50280.2 24.113 282 189 10 140 409 10 278 1.22e-12 68.9

Find 147 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACACTTCTTAACTCTATT+TGG 0.145351 tig0027169:-43172 Msa1432750:five_prime_UTR
ATCTTATCACTGCATGTTAT+TGG 0.178748 tig0027169:-41307 Msa1432750:CDS
CACTCCTTGTAGGATGATTT+TGG 0.205974 tig0027169:+40763 None:intergenic
TCTTCCTTGATGTACGTATT+TGG 0.224399 tig0027169:+41434 None:intergenic
GTGCAATCCATAGCTGTATT+TGG 0.262670 tig0027169:-43214 Msa1432750:five_prime_UTR
TGGGGTGTGGATAGGGTTGA+TGG 0.279515 tig0027169:-40569 Msa1432750:three_prime_UTR
TGTGTCTTTGGTCATGGTTT+TGG 0.289871 tig0027169:-40473 Msa1432750:three_prime_UTR
GATATAACACTTGATCAATT+TGG 0.295091 tig0027169:-42796 Msa1432750:CDS
TTGTTGCTTGTCGGATATTA+TGG 0.296854 tig0027169:-41191 Msa1432750:CDS
GCATGTTTAAGAACCTTAAC+AGG 0.298889 tig0027169:+42937 None:intergenic
AGACGTGAACGGTGTGTAAC+AGG 0.307672 tig0027169:-40347 Msa1432750:three_prime_UTR
ACAAACCTCTTCATATCTTC+AGG 0.311975 tig0027169:+40813 None:intergenic
AACTCAACTGCATAACCTAA+TGG 0.312827 tig0027169:+41113 None:intergenic
GGGTCTTTCTCTTTCATTGA+TGG 0.316385 tig0027169:+42958 None:intergenic
CCATTCAACCTGTATGGAAT+AGG 0.323735 tig0027169:-41526 Msa1432750:CDS
CTGCCGCACAAAAGCATTGC+AGG 0.340219 tig0027169:-41282 Msa1432750:CDS
TTCTTATTTGGGGTGTGGAT+AGG 0.343255 tig0027169:-40577 Msa1432750:three_prime_UTR
GAACGTCGTATAGAATTTCT+TGG 0.347222 tig0027169:+42743 None:intergenic
TCTTATTTGGGGTGTGGATA+GGG 0.353820 tig0027169:-40576 Msa1432750:three_prime_UTR
TGGTAATTGTATTGCTTTCT+CGG 0.356387 tig0027169:-41171 Msa1432750:CDS
AGAGGAGTACATGCATTCTT+TGG 0.363021 tig0027169:-41063 Msa1432750:CDS
ATCCAGCTGCAGGAAGGATT+AGG 0.365308 tig0027169:-42888 Msa1432750:CDS
ATCACAAGCTACAAACTATA+AGG 0.369159 tig0027169:+40719 None:intergenic
GAGCTTTGTGGAAACCCATT+AGG 0.375078 tig0027169:-41128 Msa1432750:CDS
CGTGACTGCATGGGCAGAAA+TGG 0.385863 tig0027169:-41570 Msa1432750:CDS
AAGTTCTCAGAAGTAAATTT+CGG 0.389814 tig0027169:-40963 Msa1432750:CDS
TCCAGCTGCAGGAAGGATTA+GGG 0.391602 tig0027169:-42887 Msa1432750:CDS
AACAAATGAGAAAAGATTAG+AGG 0.400929 tig0027169:+40540 None:intergenic
TCCCTAATCCTTCCTGCAGC+TGG 0.402307 tig0027169:+42886 None:intergenic
AAACTAGGAGCACTCCTTGT+AGG 0.407155 tig0027169:+40753 None:intergenic
GGGTGTGGATAGGGTTGATG+GGG 0.407349 tig0027169:-40567 Msa1432750:three_prime_UTR
CATTCAACCTGTATGGAATA+GGG 0.408225 tig0027169:-41525 Msa1432750:CDS
GCTAACCCTTTGTTGCTTGT+CGG 0.409807 tig0027169:-41200 Msa1432750:CDS
GTGTATGGTGGAGCAGTAAA+AGG 0.414611 tig0027169:-40927 Msa1432750:CDS
GGAGCAGTAAAAGGTGGAAT+TGG 0.418235 tig0027169:-40918 Msa1432750:CDS
GGGTGCTGGGCGAAAGTTGA+TGG 0.420210 tig0027169:-42863 Msa1432750:CDS
AACCTTCCATTCAACCTGTA+TGG 0.427456 tig0027169:-41532 Msa1432750:CDS
TCATGAGGAGTGGGTGCAGC+TGG 0.430177 tig0027169:+43060 None:intergenic
CAGCTCCATCGCCCATTGTA+TGG 0.433098 tig0027169:+41606 None:intergenic
GGAAGGATTAGGGAGGGTGC+TGG 0.433324 tig0027169:-42877 Msa1432750:CDS
ACTCAACTGCATAACCTAAT+GGG 0.436624 tig0027169:+41114 None:intergenic
GAAAGATGTTTATTTACAAA+GGG 0.441292 tig0027169:-41020 Msa1432750:CDS
GAAATTCTATACGACGTTCC+AGG 0.442306 tig0027169:-42739 Msa1432750:CDS
CTGTGAAGAAGAAAGATTGA+TGG 0.446115 tig0027169:+41393 None:intergenic
GGAAAGATGTTTATTTACAA+AGG 0.447433 tig0027169:-41021 Msa1432750:CDS
CCTATTCCATACAGGTTGAA+TGG 0.453015 tig0027169:+41526 None:intergenic
ATCATTCGTACCTCTTCATC+TGG 0.459290 tig0027169:+41254 None:intergenic
AAGAATGCAAAAGGAGAAGA+AGG 0.460427 tig0027169:-40861 Msa1432750:CDS
CATAGCTGCGATTCGTCGCC+TGG 0.472547 tig0027169:-41363 Msa1432750:CDS
TACAAAGGGAAGAACTTGTA+TGG 0.478317 tig0027169:-41006 Msa1432750:CDS
AGAATCTCCCTATTCCATAC+AGG 0.480630 tig0027169:+41518 None:intergenic
ATGGTGGATTGCACTGGGGA+GGG 0.483447 tig0027169:-42844 Msa1432750:CDS
CAAACCTCTTCATATCTTCA+GGG 0.484202 tig0027169:+40814 None:intergenic
GCATCGTTAATGCACGTTCG+AGG 0.486409 tig0027169:-41229 Msa1432750:CDS
TCAATAATATATTCTGATCC+TGG 0.488870 tig0027169:+42721 None:intergenic
TCCTTGATGTACGTATTTGG+CGG 0.490692 tig0027169:+41437 None:intergenic
AAGACCTGTATGAGTCGAAC+GGG 0.490760 tig0027169:+42694 None:intergenic
CAAGTGTGGTGGTTTCATAA+TGG 0.491746 tig0027169:-41642 Msa1432750:CDS
GAACTTGTATGGTGTCAGAT+TGG 0.493406 tig0027169:-40995 Msa1432750:CDS
GTTTATTACTATCCAGCTGC+AGG 0.495084 tig0027169:-42898 Msa1432750:CDS
TGAATCATTTATATATCAAT+CGG 0.496551 tig0027169:+40693 None:intergenic
GGTGTGGATAGGGTTGATGG+GGG 0.496722 tig0027169:-40566 Msa1432750:three_prime_UTR
TGGAAGTACCAACTCTGGTT+GGG 0.497528 tig0027169:+43080 None:intergenic
ATTCTTATGCGGAACAAGAA+AGG 0.498417 tig0027169:+40878 None:intergenic
GGATGGGGTGAAGCTGTGTA+TGG 0.499977 tig0027169:-40942 Msa1432750:CDS
ATGGAATAGGGAGATTCTAA+TGG 0.500842 tig0027169:-41513 Msa1432750:CDS
GAGAAAGGTGAAGTGGAACC+AGG 0.501342 tig0027169:+41345 None:intergenic
GGGGTGTGGATAGGGTTGAT+GGG 0.505527 tig0027169:-40568 Msa1432750:three_prime_UTR
CATACAATGGGCGATGGAGC+TGG 0.513365 tig0027169:-41605 Msa1432750:CDS
ATTTCTTGGAAGCAAGGGAA+TGG 0.515708 tig0027169:+42757 None:intergenic
GATGGTGGATTGCACTGGGG+AGG 0.517131 tig0027169:-42845 Msa1432750:CDS
GTTCCGCATAAGAATGCAAA+AGG 0.518570 tig0027169:-40870 Msa1432750:CDS
CTGGAAGTACCAACTCTGGT+TGG 0.519392 tig0027169:+43079 None:intergenic
TATAGAATTTCTTGGAAGCA+AGG 0.520340 tig0027169:+42751 None:intergenic
TTCCATACAGGTTGAATGGA+AGG 0.520405 tig0027169:+41530 None:intergenic
GAAGGATTAGGGAGGGTGCT+GGG 0.522109 tig0027169:-42876 Msa1432750:CDS
CGTATGCAGTGCATTGAGAA+AGG 0.526515 tig0027169:+41330 None:intergenic
AGTTTAACTTCATGAGGAGT+GGG 0.527780 tig0027169:+43051 None:intergenic
AAGTTGATGGTGGATTGCAC+TGG 0.528428 tig0027169:-42850 Msa1432750:CDS
GAAAGAGAAAGACCCTGTTA+AGG 0.531454 tig0027169:-42950 Msa1432750:CDS
GGTTGCAAGTAACGTGTGGC+GGG 0.531576 tig0027169:+41483 None:intergenic
CCGTCCCGTTCGACTCATAC+AGG 0.533062 tig0027169:-42698 Msa1432750:intron
CTCCGCACTGTGAACAATAG+TGG 0.533503 tig0027169:+43105 None:intergenic
CTCAGAAGTAAATTTCGGAT+GGG 0.534991 tig0027169:-40958 Msa1432750:CDS
TCTCAGAAGTAAATTTCGGA+TGG 0.535849 tig0027169:-40959 Msa1432750:CDS
TGAGACAATTCGTGACTGCA+TGG 0.535891 tig0027169:-41580 Msa1432750:CDS
AAAGAGCTAAATGATATGCT+TGG 0.536007 tig0027169:-40789 Msa1432750:CDS
TTGAGCTTGAACCATACAAT+GGG 0.537449 tig0027169:-41617 Msa1432750:CDS
ACCACTGCAAAGGAGCTTTG+TGG 0.545376 tig0027169:-41140 Msa1432750:CDS
CCGCACAAAAGCATTGCAGG+TGG 0.552363 tig0027169:-41279 Msa1432750:CDS
GAGACAATTCGTGACTGCAT+GGG 0.553285 tig0027169:-41579 Msa1432750:CDS
ATGTGTGTGCAATAATGAAA+AGG 0.554289 tig0027169:-40641 Msa1432750:three_prime_UTR
ACACCGTTCACGTCTTGTGT+TGG 0.555223 tig0027169:+40355 None:intergenic
AATATCCGACAAGCAACAAA+GGG 0.562922 tig0027169:+41195 None:intergenic
GAGTTTAACTTCATGAGGAG+TGG 0.565045 tig0027169:+43050 None:intergenic
TCCACAAAGCTCCTTTGCAG+TGG 0.567433 tig0027169:+41139 None:intergenic
CATTGTATGGTTCAAGCTCA+AGG 0.569460 tig0027169:+41619 None:intergenic
AAGGTAGACAAGATGAAACT+AGG 0.571958 tig0027169:+40738 None:intergenic
GTGATAACCAAATACAGCTA+TGG 0.572085 tig0027169:+43207 None:intergenic
ATAGAATTTCTTGGAAGCAA+GGG 0.573708 tig0027169:+42752 None:intergenic
GGCAGCAGTCACCACTGCAA+AGG 0.576150 tig0027169:-41150 Msa1432750:CDS
TCAGAAGTAAATTTCGGATG+GGG 0.577426 tig0027169:-40957 Msa1432750:CDS
CAACAAAACAAAATTGTGCT+AGG 0.577670 tig0027169:-40323 Msa1432750:three_prime_UTR
GCAGCTGGAAGTACCAACTC+TGG 0.584510 tig0027169:+43075 None:intergenic
GACGTGAACGGTGTGTAACA+GGG 0.586024 tig0027169:-40346 Msa1432750:three_prime_UTR
CTTGAGCTTGAACCATACAA+TGG 0.589328 tig0027169:-41618 Msa1432750:CDS
AGCAAAAGCTCAAGTTACAG+AGG 0.589925 tig0027169:-41081 Msa1432750:CDS
ATAAATAAATATGAAAAGCA+AGG 0.590393 tig0027169:-40606 Msa1432750:three_prime_UTR
TAATATCCGACAAGCAACAA+AGG 0.591430 tig0027169:+41194 None:intergenic
GCAGGTGGAGCCAGATGAAG+AGG 0.592868 tig0027169:-41264 Msa1432750:CDS
TCTTGGAAGCAAGGGAATGG+AGG 0.599063 tig0027169:+42760 None:intergenic
AGTTGATGGTGGATTGCACT+GGG 0.599197 tig0027169:-42849 Msa1432750:CDS
GAGAGGAGTTTAACTTCATG+AGG 0.599961 tig0027169:+43045 None:intergenic
TGGGGTGAAGCTGTGTATGG+TGG 0.601746 tig0027169:-40939 Msa1432750:CDS
TGACGAAAGACAAATATGAC+AGG 0.607837 tig0027169:+42985 None:intergenic
ATTACTATCCAGCTGCAGGA+AGG 0.614273 tig0027169:-42894 Msa1432750:CDS
GTCTCTAGTTTGTACGTACG+TGG 0.614654 tig0027169:-43247 None:intergenic
GCGGAGGTCCCAACCAGAGT+TGG 0.615833 tig0027169:-43088 Msa1432750:CDS
TGACAGGAAGATTGAAACGA+AGG 0.615959 tig0027169:+43001 None:intergenic
TAAGACCTGTATGAGTCGAA+CGG 0.619194 tig0027169:+42693 None:intergenic
GTGCATTGAGAAAGGTGAAG+TGG 0.620215 tig0027169:+41338 None:intergenic
TCCGCCAAATACGTACATCA+AGG 0.620796 tig0027169:-41438 Msa1432750:CDS
GCCTTCTTGATCATCTATGT+CGG 0.622425 tig0027169:+43023 None:intergenic
GTAACCAAAATCATCCTACA+AGG 0.623110 tig0027169:-40767 Msa1432750:CDS
TGGTTGCAAGTAACGTGTGG+CGG 0.626899 tig0027169:+41482 None:intergenic
TTGAACCATACAATGGGCGA+TGG 0.631288 tig0027169:-41611 Msa1432750:CDS
TGCTGGGCGAAAGTTGATGG+TGG 0.635555 tig0027169:-42860 Msa1432750:CDS
CCTGTATGAGTCGAACGGGA+CGG 0.638129 tig0027169:+42698 None:intergenic
GTGTGCAATAATGAAAAGGA+AGG 0.644525 tig0027169:-40637 Msa1432750:three_prime_UTR
TCCGACATAGATGATCAAGA+AGG 0.646509 tig0027169:-43024 Msa1432750:CDS
AAACCAACACAAGACGTGAA+CGG 0.648957 tig0027169:-40358 Msa1432750:three_prime_UTR
AAACCTCTTCATATCTTCAG+GGG 0.652653 tig0027169:+40815 None:intergenic
TATGGTGGAGCAGTAAAAGG+TGG 0.653295 tig0027169:-40924 Msa1432750:CDS
CATGTTTAAGAACCTTAACA+GGG 0.656931 tig0027169:+42938 None:intergenic
AGCTGCAGGAAGGATTAGGG+AGG 0.663235 tig0027169:-42884 Msa1432750:CDS
AGAATGCAAAAGGAGAAGAA+GGG 0.663405 tig0027169:-40860 Msa1432750:CDS
GTTACACGTCTCAAGTGTGG+TGG 0.666120 tig0027169:-41653 Msa1432750:CDS
AACCTCTTCATATCTTCAGG+GGG 0.666136 tig0027169:+40816 None:intergenic
CAGGTTACACGTCTCAAGTG+TGG 0.668116 tig0027169:-41656 Msa1432750:CDS
CGATGGTTGCAAGTAACGTG+TGG 0.669479 tig0027169:+41479 None:intergenic
GTATTTGTTCGTATTCACGA+TGG 0.674672 tig0027169:+41462 None:intergenic
CTTGATCATCTATGTCGGAG+AGG 0.675142 tig0027169:+43028 None:intergenic
CACTATTGTTCACAGTGCGG+AGG 0.677341 tig0027169:-43104 Msa1432750:CDS
GCATGGGCAGAAATGGCTCG+AGG 0.681407 tig0027169:-41563 Msa1432750:CDS
GCTGCAGGAAGGATTAGGGA+GGG 0.683826 tig0027169:-42883 Msa1432750:CDS
CACCACTATTGTTCACAGTG+CGG 0.732056 tig0027169:-43107 Msa1432750:CDS
GTTGATGGTGGATTGCACTG+GGG 0.762765 tig0027169:-42848 Msa1432750:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGTAATTAATATAAATTCAT+AGG + tig0027169:41368-41387 None:intergenic 10.0%
!! TGATCAATAATTAAATTTCA+AGG + tig0027169:40896-40915 None:intergenic 15.0%
!!! TATATAATTTATTTGTTGTG+TGG + tig0027169:41133-41152 None:intergenic 15.0%
!! AAATAATTGCATTATATAAG+TGG + tig0027169:41309-41328 None:intergenic 15.0%
!! TGAATCATTTATATATCAAT+CGG + tig0027169:42855-42874 None:intergenic 15.0%
!! ATAAATAAATATGAAAAGCA+AGG - tig0027169:42939-42958 Msa1432750:CDS 15.0%
!!! TTTGTGTCTTTTTTTTTAAT+GGG - tig0027169:43149-43168 Msa1432750:five_prime_UTR 15.0%
!!! AAACATCCTTGAAATTTTAT+AGG - tig0027169:40921-40940 Msa1432750:CDS 20.0%
!!! TTTATTCCAAGTCTAAAATT+CGG + tig0027169:41062-41081 None:intergenic 20.0%
!! TTATCCCAAAAAAAAAATGA+GGG + tig0027169:41527-41546 None:intergenic 20.0%
!! TTTATCCCAAAAAAAAAATG+AGG + tig0027169:41528-41547 None:intergenic 20.0%
!!! ACCATTTTTCGTAAAAAAAT+TGG + tig0027169:41553-41572 None:intergenic 20.0%
!! ACTTAAAAATAGTGTAAAGT+TGG + tig0027169:41582-41601 None:intergenic 20.0%
!! TAACATATACATACATCTTT+AGG - tig0027169:41837-41856 Msa1432750:intron 20.0%
!! GATAATTAGAATTAAATGAC+AGG - tig0027169:41870-41889 Msa1432750:intron 20.0%
!! GAAAGATGTTTATTTACAAA+GGG - tig0027169:42525-42544 Msa1432750:intron 20.0%
!!! CTTTGTGTCTTTTTTTTTAA+TGG - tig0027169:43148-43167 Msa1432750:five_prime_UTR 20.0%
! TCATGCTATCATAAAAAAGA+AGG + tig0027169:40409-40428 None:intergenic 25.0%
! GATATAACACTTGATCAATT+TGG - tig0027169:40749-40768 Msa1432750:CDS 25.0%
! TCAATAATATATTCTGATCC+TGG + tig0027169:40827-40846 None:intergenic 25.0%
! AAGAGACCTATAAAATTTCA+AGG + tig0027169:40930-40949 None:intergenic 25.0%
!! TGATCTCATTACAATCATTT+TGG + tig0027169:40994-41013 None:intergenic 25.0%
! AAAATGATTGTAATGAGATC+AGG - tig0027169:40993-41012 Msa1432750:CDS 25.0%
! AAATGATTGTAATGAGATCA+GGG - tig0027169:40994-41013 Msa1432750:CDS 25.0%
!! TTATTCCAAGTCTAAAATTC+GGG + tig0027169:41061-41080 None:intergenic 25.0%
!! GAATTTTAGACTTGGAATAA+AGG - tig0027169:41061-41080 Msa1432750:CDS 25.0%
!! ATTATCATGCATTAACTTGA+TGG + tig0027169:41472-41491 None:intergenic 25.0%
! TATCCCAAAAAAAAAATGAG+GGG + tig0027169:41526-41545 None:intergenic 25.0%
!!! GCCAATTTTTTTACGAAAAA+TGG - tig0027169:41549-41568 Msa1432750:CDS 25.0%
!!! TATTAGTCTTTGAAGTTATC+GGG + tig0027169:41622-41641 None:intergenic 25.0%
!!! CTATTAGTCTTTGAAGTTAT+CGG + tig0027169:41623-41642 None:intergenic 25.0%
! TTTCACATTAAATATGCCTT+GGG + tig0027169:41683-41702 None:intergenic 25.0%
! ATTTCACATTAAATATGCCT+TGG + tig0027169:41684-41703 None:intergenic 25.0%
!!! TCAATGACATTAGTCTTTTT+AGG + tig0027169:41794-41813 None:intergenic 25.0%
! GGAAAGATGTTTATTTACAA+AGG - tig0027169:42524-42543 Msa1432750:intron 25.0%
! AAGTTCTCAGAAGTAAATTT+CGG - tig0027169:42582-42601 Msa1432750:intron 25.0%
!!! CAAGGTCTATTTTCTTATTT+GGG - tig0027169:42957-42976 Msa1432750:CDS 25.0%
!!! AAGGTCTATTTTCTTATTTG+GGG - tig0027169:42958-42977 Msa1432750:CDS 25.0%
! AACAAATGAGAAAAGATTAG+AGG + tig0027169:43008-43027 None:intergenic 25.0%
! GAATGAAAGAGAAAAAAGAA+AGG + tig0027169:43119-43138 None:intergenic 25.0%
!!! CTTTTTTCTCTTTCATTCTT+TGG - tig0027169:43121-43140 Msa1432750:CDS 25.0%
CAACACTTCTTAACTCTATT+TGG - tig0027169:40373-40392 Msa1432750:three_prime_UTR 30.0%
GCTATCATAAAAAAGAAGGA+GGG + tig0027169:40405-40424 None:intergenic 30.0%
TGCTATCATAAAAAAGAAGG+AGG + tig0027169:40406-40425 None:intergenic 30.0%
CATGTTTAAGAACCTTAACA+GGG + tig0027169:40610-40629 None:intergenic 30.0%
ATAGAATTTCTTGGAAGCAA+GGG + tig0027169:40796-40815 None:intergenic 30.0%
TATAGAATTTCTTGGAAGCA+AGG + tig0027169:40797-40816 None:intergenic 30.0%
! TATTCCAAGTCTAAAATTCG+GGG + tig0027169:41060-41079 None:intergenic 30.0%
!!! ACATAGTTTTGTCTATCATG+AGG + tig0027169:41087-41106 None:intergenic 30.0%
ACTTTATGATGCTTACAACA+TGG - tig0027169:41214-41233 Msa1432750:CDS 30.0%
CTTTATGATGCTTACAACAT+GGG - tig0027169:41215-41234 Msa1432750:CDS 30.0%
CTAAAGCGAACTATAATATG+AGG + tig0027169:41405-41424 None:intergenic 30.0%
CTCATATTATAGTTCGCTTT+AGG - tig0027169:41403-41422 Msa1432750:CDS 30.0%
!! TCAAAGACTAATAGCTTGTT+TGG - tig0027169:41629-41648 Msa1432750:CDS 30.0%
!! TAATAGCTTGTTTGGAATAG+CGG - tig0027169:41637-41656 Msa1432750:CDS 30.0%
ATCTTATCACTGCATGTTAT+TGG - tig0027169:42238-42257 Msa1432750:intron 30.0%
TGGTAATTGTATTGCTTTCT+CGG - tig0027169:42374-42393 Msa1432750:intron 30.0%
! GGCAGATTTTATGGTTATTA+AGG - tig0027169:42503-42522 Msa1432750:intron 30.0%
AAAGAGCTAAATGATATGCT+TGG - tig0027169:42756-42775 Msa1432750:CDS 30.0%
ATCACAAGCTACAAACTATA+AGG + tig0027169:42829-42848 None:intergenic 30.0%
ATGTGTGTGCAATAATGAAA+AGG - tig0027169:42904-42923 Msa1432750:CDS 30.0%
! GCAAGGTCTATTTTCTTATT+TGG - tig0027169:42956-42975 Msa1432750:CDS 30.0%
!!! AGTTTGTTTTGTTGTGTCTT+TGG - tig0027169:43060-43079 Msa1432750:CDS 30.0%
CAACAAAACAAAATTGTGCT+AGG - tig0027169:43222-43241 Msa1432750:five_prime_UTR 30.0%
GTGATAACCAAATACAGCTA+TGG + tig0027169:40341-40360 None:intergenic 35.0%
! AGTTTAACTTCATGAGGAGT+GGG + tig0027169:40497-40516 None:intergenic 35.0%
TGACGAAAGACAAATATGAC+AGG + tig0027169:40563-40582 None:intergenic 35.0%
GCATGTTTAAGAACCTTAAC+AGG + tig0027169:40611-40630 None:intergenic 35.0%
GAACGTCGTATAGAATTTCT+TGG + tig0027169:40805-40824 None:intergenic 35.0%
! ATTCCAAGTCTAAAATTCGG+GGG + tig0027169:41059-41078 None:intergenic 35.0%
GTGCATTGTAAAGTTGTATG+AGG + tig0027169:41251-41270 None:intergenic 35.0%
! AAGTGTTGTAAGTGTAGCAT+TGG + tig0027169:41286-41305 None:intergenic 35.0%
TTTAGGCCTTAGAATGTGTT+AGG - tig0027169:41420-41439 Msa1432750:CDS 35.0%
!!! TGGACCCCTCATTTTTTTTT+TGG - tig0027169:41519-41538 Msa1432750:CDS 35.0%
!!! GGACCCCTCATTTTTTTTTT+GGG - tig0027169:41520-41539 Msa1432750:CDS 35.0%
TTGAGCTTGAACCATACAAT+GGG - tig0027169:41928-41947 Msa1432750:intron 35.0%
CATTCAACCTGTATGGAATA+GGG - tig0027169:42020-42039 Msa1432750:intron 35.0%
ATGGAATAGGGAGATTCTAA+TGG - tig0027169:42032-42051 Msa1432750:intron 35.0%
GTATTTGTTCGTATTCACGA+TGG + tig0027169:42086-42105 None:intergenic 35.0%
TCTTCCTTGATGTACGTATT+TGG + tig0027169:42114-42133 None:intergenic 35.0%
CTGTGAAGAAGAAAGATTGA+TGG + tig0027169:42155-42174 None:intergenic 35.0%
AATATCCGACAAGCAACAAA+GGG + tig0027169:42353-42372 None:intergenic 35.0%
TAATATCCGACAAGCAACAA+AGG + tig0027169:42354-42373 None:intergenic 35.0%
! TTGTTGCTTGTCGGATATTA+TGG - tig0027169:42354-42373 Msa1432750:intron 35.0%
ACTCAACTGCATAACCTAAT+GGG + tig0027169:42434-42453 None:intergenic 35.0%
AACTCAACTGCATAACCTAA+TGG + tig0027169:42435-42454 None:intergenic 35.0%
! GCATTCTTTGGCAGATTTTA+TGG - tig0027169:42494-42513 Msa1432750:intron 35.0%
TACAAAGGGAAGAACTTGTA+TGG - tig0027169:42539-42558 Msa1432750:intron 35.0%
TCTCAGAAGTAAATTTCGGA+TGG - tig0027169:42586-42605 Msa1432750:intron 35.0%
CTCAGAAGTAAATTTCGGAT+GGG - tig0027169:42587-42606 Msa1432750:intron 35.0%
TCAGAAGTAAATTTCGGATG+GGG - tig0027169:42588-42607 Msa1432750:intron 35.0%
! GTGGAATTGGATCTTTTCTT+GGG - tig0027169:42640-42659 Msa1432750:intron 35.0%
! TGGAATTGGATCTTTTCTTG+GGG - tig0027169:42641-42660 Msa1432750:intron 35.0%
ATTCTTATGCGGAACAAGAA+AGG + tig0027169:42670-42689 None:intergenic 35.0%
! TCTCCTTTTGCATTCTTATG+CGG + tig0027169:42681-42700 None:intergenic 35.0%
AAGAATGCAAAAGGAGAAGA+AGG - tig0027169:42684-42703 Msa1432750:intron 35.0%
AGAATGCAAAAGGAGAAGAA+GGG - tig0027169:42685-42704 Msa1432750:intron 35.0%
AAACCTCTTCATATCTTCAG+GGG + tig0027169:42733-42752 None:intergenic 35.0%
CAAACCTCTTCATATCTTCA+GGG + tig0027169:42734-42753 None:intergenic 35.0%
ACAAACCTCTTCATATCTTC+AGG + tig0027169:42735-42754 None:intergenic 35.0%
GTAACCAAAATCATCCTACA+AGG - tig0027169:42778-42797 Msa1432750:CDS 35.0%
AAGGTAGACAAGATGAAACT+AGG + tig0027169:42810-42829 None:intergenic 35.0%
GTGTGCAATAATGAAAAGGA+AGG - tig0027169:42908-42927 Msa1432750:CDS 35.0%
!! CTATTTTCTTATTTGGGGTG+TGG - tig0027169:42963-42982 Msa1432750:CDS 35.0%
!! TTTTGTTGTGTCTTTGGTCA+TGG - tig0027169:43066-43085 Msa1432750:CDS 35.0%
GTGCAATCCATAGCTGTATT+TGG - tig0027169:40331-40350 Msa1432750:three_prime_UTR 40.0%
! GAGTTTAACTTCATGAGGAG+TGG + tig0027169:40498-40517 None:intergenic 40.0%
! GAGAGGAGTTTAACTTCATG+AGG + tig0027169:40503-40522 None:intergenic 40.0%
TCCGACATAGATGATCAAGA+AGG - tig0027169:40521-40540 Msa1432750:three_prime_UTR 40.0%
GCCTTCTTGATCATCTATGT+CGG + tig0027169:40525-40544 None:intergenic 40.0%
TGACAGGAAGATTGAAACGA+AGG + tig0027169:40547-40566 None:intergenic 40.0%
GGGTCTTTCTCTTTCATTGA+TGG + tig0027169:40590-40609 None:intergenic 40.0%
GAAAGAGAAAGACCCTGTTA+AGG - tig0027169:40595-40614 Msa1432750:three_prime_UTR 40.0%
GTTTATTACTATCCAGCTGC+AGG - tig0027169:40647-40666 Msa1432750:three_prime_UTR 40.0%
ATTTCTTGGAAGCAAGGGAA+TGG + tig0027169:40791-40810 None:intergenic 40.0%
GAAATTCTATACGACGTTCC+AGG - tig0027169:40806-40825 Msa1432750:CDS 40.0%
TAAGACCTGTATGAGTCGAA+CGG + tig0027169:40855-40874 None:intergenic 40.0%
! TGTTGTAAGTGTAGCATTGG+CGG + tig0027169:41283-41302 None:intergenic 40.0%
CTTTCCATCTCATTCAGTGT+CGG + tig0027169:41446-41465 None:intergenic 40.0%
AAGCTTTCTAACCAGTCCAA+CGG - tig0027169:41496-41515 Msa1432750:CDS 40.0%
CAAGTGTGGTGGTTTCATAA+TGG - tig0027169:41903-41922 Msa1432750:intron 40.0%
CATTGTATGGTTCAAGCTCA+AGG + tig0027169:41929-41948 None:intergenic 40.0%
CTTGAGCTTGAACCATACAA+TGG - tig0027169:41927-41946 Msa1432750:intron 40.0%
AACCTTCCATTCAACCTGTA+TGG - tig0027169:42013-42032 Msa1432750:intron 40.0%
! TTCCATACAGGTTGAATGGA+AGG + tig0027169:42018-42037 None:intergenic 40.0%
! CCTATTCCATACAGGTTGAA+TGG + tig0027169:42022-42041 None:intergenic 40.0%
CCATTCAACCTGTATGGAAT+AGG - tig0027169:42019-42038 Msa1432750:intron 40.0%
AGAATCTCCCTATTCCATAC+AGG + tig0027169:42030-42049 None:intergenic 40.0%
TCCTTGATGTACGTATTTGG+CGG + tig0027169:42111-42130 None:intergenic 40.0%
ATCATTCGTACCTCTTCATC+TGG + tig0027169:42294-42313 None:intergenic 40.0%
AGCAAAAGCTCAAGTTACAG+AGG - tig0027169:42464-42483 Msa1432750:intron 40.0%
! AGAGGAGTACATGCATTCTT+TGG - tig0027169:42482-42501 Msa1432750:intron 40.0%
! GAACTTGTATGGTGTCAGAT+TGG - tig0027169:42550-42569 Msa1432750:intron 40.0%
! GGTGGAATTGGATCTTTTCT+TGG - tig0027169:42639-42658 Msa1432750:intron 40.0%
! GGAATTGGATCTTTTCTTGG+GGG - tig0027169:42642-42661 Msa1432750:intron 40.0%
GTTCCGCATAAGAATGCAAA+AGG - tig0027169:42675-42694 Msa1432750:intron 40.0%
AACCTCTTCATATCTTCAGG+GGG + tig0027169:42732-42751 None:intergenic 40.0%
! CACTCCTTGTAGGATGATTT+TGG + tig0027169:42785-42804 None:intergenic 40.0%
! TTCTTATTTGGGGTGTGGAT+AGG - tig0027169:42968-42987 Msa1432750:CDS 40.0%
! TCTTATTTGGGGTGTGGATA+GGG - tig0027169:42969-42988 Msa1432750:CDS 40.0%
! TGTGTCTTTGGTCATGGTTT+TGG - tig0027169:43072-43091 Msa1432750:CDS 40.0%
! GTGTCTTTGGTCATGGTTTT+GGG - tig0027169:43073-43092 Msa1432750:CDS 40.0%
AAACCAACACAAGACGTGAA+CGG - tig0027169:43187-43206 Msa1432750:five_prime_UTR 40.0%
CACCACTATTGTTCACAGTG+CGG - tig0027169:40438-40457 Msa1432750:three_prime_UTR 45.0%
!! TGGAAGTACCAACTCTGGTT+GGG + tig0027169:40468-40487 None:intergenic 45.0%
CTTGATCATCTATGTCGGAG+AGG + tig0027169:40520-40539 None:intergenic 45.0%
ATTACTATCCAGCTGCAGGA+AGG - tig0027169:40651-40670 Msa1432750:three_prime_UTR 45.0%
!! AAGTTGATGGTGGATTGCAC+TGG - tig0027169:40695-40714 Msa1432750:three_prime_UTR 45.0%
!! AGTTGATGGTGGATTGCACT+GGG - tig0027169:40696-40715 Msa1432750:three_prime_UTR 45.0%
AAGACCTGTATGAGTCGAAC+GGG + tig0027169:40854-40873 None:intergenic 45.0%
GATTGTAATGAGATCAGGGC+TGG - tig0027169:40998-41017 Msa1432750:CDS 45.0%
TGTCGGCCTAACACATTCTA+AGG + tig0027169:41429-41448 None:intergenic 45.0%
!! TTTGCATTTCTAGCGTCCCA+AGG - tig0027169:41664-41683 Msa1432750:intron 45.0%
CATTCACCGCTAAAACAGAC+AGG - tig0027169:41753-41772 Msa1432750:intron 45.0%
!! GTAGCTCCTGTCTGTTTTAG+CGG + tig0027169:41762-41781 None:intergenic 45.0%
TTGAACCATACAATGGGCGA+TGG - tig0027169:41934-41953 Msa1432750:intron 45.0%
TGAGACAATTCGTGACTGCA+TGG - tig0027169:41965-41984 Msa1432750:intron 45.0%
GAGACAATTCGTGACTGCAT+GGG - tig0027169:41966-41985 Msa1432750:intron 45.0%
TCCGCCAAATACGTACATCA+AGG - tig0027169:42107-42126 Msa1432750:intron 45.0%
GTGCATTGAGAAAGGTGAAG+TGG + tig0027169:42210-42229 None:intergenic 45.0%
CGTATGCAGTGCATTGAGAA+AGG + tig0027169:42218-42237 None:intergenic 45.0%
! GCTAACCCTTTGTTGCTTGT+CGG - tig0027169:42345-42364 Msa1432750:intron 45.0%
! GAGCTTTGTGGAAACCCATT+AGG - tig0027169:42417-42436 Msa1432750:intron 45.0%
! GTGTATGGTGGAGCAGTAAA+AGG - tig0027169:42618-42637 Msa1432750:intron 45.0%
! TATGGTGGAGCAGTAAAAGG+TGG - tig0027169:42621-42640 Msa1432750:intron 45.0%
GGAGCAGTAAAAGGTGGAAT+TGG - tig0027169:42627-42646 Msa1432750:intron 45.0%
!! AAACTAGGAGCACTCCTTGT+AGG + tig0027169:42795-42814 None:intergenic 45.0%
CTCCGCACTGTGAACAATAG+TGG + tig0027169:40443-40462 None:intergenic 50.0%
CACTATTGTTCACAGTGCGG+AGG - tig0027169:40441-40460 Msa1432750:three_prime_UTR 50.0%
!! CTGGAAGTACCAACTCTGGT+TGG + tig0027169:40469-40488 None:intergenic 50.0%
ATCCAGCTGCAGGAAGGATT+AGG - tig0027169:40657-40676 Msa1432750:three_prime_UTR 50.0%
TCCAGCTGCAGGAAGGATTA+GGG - tig0027169:40658-40677 Msa1432750:three_prime_UTR 50.0%
!! GTTGATGGTGGATTGCACTG+GGG - tig0027169:40697-40716 Msa1432750:three_prime_UTR 50.0%
TCTTGGAAGCAAGGGAATGG+AGG + tig0027169:40788-40807 None:intergenic 50.0%
!! GTTTGGGTTGCCTTACCCTT+AGG + tig0027169:41024-41043 None:intergenic 50.0%
! AGGCCCCCGAATTTTAGACT+TGG - tig0027169:41053-41072 Msa1432750:CDS 50.0%
TAGGCCGACACTGAATGAGA+TGG - tig0027169:41439-41458 Msa1432750:CDS 50.0%
CTTTCTAACCAGTCCAACGG+TGG - tig0027169:41499-41518 Msa1432750:CDS 50.0%
AAAAATGAGGGGTCCACCGT+TGG + tig0027169:41515-41534 None:intergenic 50.0%
CAGGTTACACGTCTCAAGTG+TGG - tig0027169:41889-41908 Msa1432750:intron 50.0%
GTTACACGTCTCAAGTGTGG+TGG - tig0027169:41892-41911 Msa1432750:intron 50.0%
TGGTTGCAAGTAACGTGTGG+CGG + tig0027169:42066-42085 None:intergenic 50.0%
CGATGGTTGCAAGTAACGTG+TGG + tig0027169:42069-42088 None:intergenic 50.0%
GAGAAAGGTGAAGTGGAACC+AGG + tig0027169:42203-42222 None:intergenic 50.0%
! CCACCTGCAATGCTTTTGTG+CGG + tig0027169:42269-42288 None:intergenic 50.0%
GCATCGTTAATGCACGTTCG+AGG - tig0027169:42316-42335 Msa1432750:intron 50.0%
ACCACTGCAAAGGAGCTTTG+TGG - tig0027169:42405-42424 Msa1432750:intron 50.0%
TCCACAAAGCTCCTTTGCAG+TGG + tig0027169:42409-42428 None:intergenic 50.0%
TCTTCAGGGGGCAAGCAAAT+TGG + tig0027169:42720-42739 None:intergenic 50.0%
TGCCCCCTGAAGATATGAAG+AGG - tig0027169:42727-42746 Msa1432750:CDS 50.0%
ACACCGTTCACGTCTTGTGT+TGG + tig0027169:43193-43212 None:intergenic 50.0%
AGACGTGAACGGTGTGTAAC+AGG - tig0027169:43198-43217 Msa1432750:five_prime_UTR 50.0%
GACGTGAACGGTGTGTAACA+GGG - tig0027169:43199-43218 Msa1432750:five_prime_UTR 50.0%
!! GCAGCTGGAAGTACCAACTC+TGG + tig0027169:40473-40492 None:intergenic 55.0%
TCCCTAATCCTTCCTGCAGC+TGG + tig0027169:40662-40681 None:intergenic 55.0%
AGCTGCAGGAAGGATTAGGG+AGG - tig0027169:40661-40680 Msa1432750:three_prime_UTR 55.0%
GCTGCAGGAAGGATTAGGGA+GGG - tig0027169:40662-40681 Msa1432750:three_prime_UTR 55.0%
!! GAAGGATTAGGGAGGGTGCT+GGG - tig0027169:40669-40688 Msa1432750:three_prime_UTR 55.0%
!! TGCTGGGCGAAAGTTGATGG+TGG - tig0027169:40685-40704 Msa1432750:three_prime_UTR 55.0%
!! ATGGTGGATTGCACTGGGGA+GGG - tig0027169:40701-40720 Msa1432750:three_prime_UTR 55.0%
CCTGTATGAGTCGAACGGGA+CGG + tig0027169:40850-40869 None:intergenic 55.0%
!! ATGAGATCAGGGCTGGCCTA+AGG - tig0027169:41005-41024 Msa1432750:CDS 55.0%
!! TGAGATCAGGGCTGGCCTAA+GGG - tig0027169:41006-41025 Msa1432750:CDS 55.0%
GCAACCCAAACCCTCGCTTT+AGG - tig0027169:41033-41052 Msa1432750:CDS 55.0%
AATTCGGGGGCCTAAAGCGA+GGG + tig0027169:41046-41065 None:intergenic 55.0%
AAATTCGGGGGCCTAAAGCG+AGG + tig0027169:41047-41066 None:intergenic 55.0%
CAGCTCCATCGCCCATTGTA+TGG + tig0027169:41942-41961 None:intergenic 55.0%
CATACAATGGGCGATGGAGC+TGG - tig0027169:41940-41959 Msa1432750:intron 55.0%
CGTGACTGCATGGGCAGAAA+TGG - tig0027169:41975-41994 Msa1432750:intron 55.0%
GGTTGCAAGTAACGTGTGGC+GGG + tig0027169:42065-42084 None:intergenic 55.0%
!! CTGCCGCACAAAAGCATTGC+AGG - tig0027169:42263-42282 Msa1432750:intron 55.0%
!! CCGCACAAAAGCATTGCAGG+TGG - tig0027169:42266-42285 Msa1432750:intron 55.0%
GGATGGGGTGAAGCTGTGTA+TGG - tig0027169:42603-42622 Msa1432750:intron 55.0%
TGGGGTGAAGCTGTGTATGG+TGG - tig0027169:42606-42625 Msa1432750:intron 55.0%
TGGGGTGTGGATAGGGTTGA+TGG - tig0027169:42976-42995 Msa1432750:CDS 55.0%
! GGGGTGTGGATAGGGTTGAT+GGG - tig0027169:42977-42996 Msa1432750:CDS 55.0%
!! GGGTGTGGATAGGGTTGATG+GGG - tig0027169:42978-42997 Msa1432750:CDS 55.0%
!! GGTGTGGATAGGGTTGATGG+GGG - tig0027169:42979-42998 Msa1432750:CDS 55.0%
TCATGAGGAGTGGGTGCAGC+TGG + tig0027169:40488-40507 None:intergenic 60.0%
! GGAAGGATTAGGGAGGGTGC+TGG - tig0027169:40668-40687 Msa1432750:three_prime_UTR 60.0%
!! GGGTGCTGGGCGAAAGTTGA+TGG - tig0027169:40682-40701 Msa1432750:three_prime_UTR 60.0%
!! GATGGTGGATTGCACTGGGG+AGG - tig0027169:40700-40719 Msa1432750:three_prime_UTR 60.0%
CCGTCCCGTTCGACTCATAC+AGG - tig0027169:40847-40866 Msa1432750:CDS 60.0%
!! TCAGGGCTGGCCTAAGGGTA+AGG - tig0027169:41011-41030 Msa1432750:CDS 60.0%
GGGGCCTAAAGCGAGGGTTT+GGG + tig0027169:41040-41059 None:intergenic 60.0%
GCATGGGCAGAAATGGCTCG+AGG - tig0027169:41982-42001 Msa1432750:intron 60.0%
CATAGCTGCGATTCGTCGCC+TGG - tig0027169:42182-42201 Msa1432750:intron 60.0%
GCAGGTGGAGCCAGATGAAG+AGG - tig0027169:42281-42300 Msa1432750:intron 60.0%
GGCAGCAGTCACCACTGCAA+AGG - tig0027169:42395-42414 Msa1432750:intron 60.0%
GCGGAGGTCCCAACCAGAGT+TGG - tig0027169:40457-40476 Msa1432750:three_prime_UTR 65.0%
GGGGGCCTAAAGCGAGGGTT+TGG + tig0027169:41041-41060 None:intergenic 65.0%
TGAGGGGTCCACCGTTGGAC+TGG + tig0027169:41510-41529 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
tig0027169 gene 40310 43257 40310 ID=Msa1432750;Name=Msa1432750
tig0027169 mRNA 40310 43257 40310 ID=Msa1432750-mRNA-1;Parent=Msa1432750;Name=Msa1432750-mRNA-1;_AED=0.25;_eAED=0.25;_QI=115|1|1|1|1|1|2|424|461
tig0027169 exon 40310 41675 40310 ID=Msa1432750-mRNA-1:exon:8259;Parent=Msa1432750-mRNA-1
tig0027169 exon 42699 43257 42699 ID=Msa1432750-mRNA-1:exon:8258;Parent=Msa1432750-mRNA-1
tig0027169 five_prime_UTR 43143 43257 43143 ID=Msa1432750-mRNA-1:five_prime_utr;Parent=Msa1432750-mRNA-1
tig0027169 CDS 42699 43142 42699 ID=Msa1432750-mRNA-1:cds;Parent=Msa1432750-mRNA-1
tig0027169 CDS 40734 41675 40734 ID=Msa1432750-mRNA-1:cds;Parent=Msa1432750-mRNA-1
tig0027169 three_prime_UTR 40310 40733 40310 ID=Msa1432750-mRNA-1:three_prime_utr;Parent=Msa1432750-mRNA-1
Gene Sequence

>Msa1432750

ATGACTTCTTCATCACCACTATTGTTCACAGTGCGGAGGTCCCAACCAGAGTTGGTACTTCCAGCTGCACCCACTCCTCATGAAGTTAAACTCCTCTCCGACATAGATGATCAAGAAGGCCTTCGTTTCAATCTTCCTGTCATATTTGTCTTTCGTCATGAACCATCAATGAAAGAGAAAGACCCTGTTAAGGTTCTTAAACATGCACTCTCACAAACACTTGTTTATTACTATCCAGCTGCAGGAAGGATTAGGGAGGGTGCTGGGCGAAAGTTGATGGTGGATTGCACTGGGGAGGGTGTCATGTTCATTGAAGCTGAAGCTGATATAACACTTGATCAATTTGGTGATGCTCTGCAACCTCCATTCCCTTGCTTCCAAGAAATTCTATACGACGTTCCAGGATCAGAATATATTATTGACCGTCCCGTTCGACTCATACAGGTTACACGTCTCAAGTGTGGTGGTTTCATAATGGCCTTGAGCTTGAACCATACAATGGGCGATGGAGCTGGTTTGAGACAATTCGTGACTGCATGGGCAGAAATGGCTCGAGGAGCAAATCAACCTTCCATTCAACCTGTATGGAATAGGGAGATTCTAATGGCAAGAGACCCGCCACACGTTACTTGCAACCATCGTGAATACGAACAAATACTTCCGCCAAATACGTACATCAAGGAAGAAGATACTACTATTGTAGTCCATCAATCTTTCTTCTTCACAGCTGCTCACATAGCTGCGATTCGTCGCCTGGTTCCACTTCACCTTTCTCAATGCACTGCATACGATCTTATCACTGCATGTTATTGGTGCTGCCGCACAAAAGCATTGCAGGTGGAGCCAGATGAAGAGGTACGAATGATGTGCATCGTTAATGCACGTTCGAGGTTTAGTGCTAACCCTTTGTTGCTTGTCGGATATTATGGTAATTGTATTGCTTTCTCGGCAGCAGTCACCACTGCAAAGGAGCTTTGTGGAAACCCATTAGGTTATGCAGTTGAGTTGATAAGAAAAGCAAAAGCTCAAGTTACAGAGGAGTACATGCATTCTTTGGCAGATTTTATGGTTATTAAGGAAAGATGTTTATTTACAAAGGGAAGAACTTGTATGGTGTCAGATTGGACTCGTGCTAAGTTCTCAGAAGTAAATTTCGGATGGGGTGAAGCTGTGTATGGTGGAGCAGTAAAAGGTGGAATTGGATCTTTTCTTGGGGGAACCTTTCTTGTTCCGCATAAGAATGCAAAAGGAGAAGAAGGGTTAATATTACCAATTTGCTTGCCCCCTGAAGATATGAAGAGGTTTGTTAAAGAGCTAAATGATATGCTTGGTAACCAAAATCATCCTACAAGGAGTGCTCCTAGTTTCATCTTGTCTACCTTATAG
Protein sequence

>Msa1432750

MTSSSPLLFTVRRSQPELVLPAAPTPHEVKLLSDIDDQEGLRFNLPVIFVFRHEPSMKEKDPVKVLKHALSQTLVYYYPAAGRIREGAGRKLMVDCTGEGVMFIEAEADITLDQFGDALQPPFPCFQEILYDVPGSEYIIDRPVRLIQVTRLKCGGFIMALSLNHTMGDGAGLRQFVTAWAEMARGANQPSIQPVWNREILMARDPPHVTCNHREYEQILPPNTYIKEEDTTIVVHQSFFFTAAHIAAIRRLVPLHLSQCTAYDLITACYWCCRTKALQVEPDEEVRMMCIVNARSRFSANPLLLVGYYGNCIAFSAAVTTAKELCGNPLGYAVELIRKAKAQVTEEYMHSLADFMVIKERCLFTKGRTCMVSDWTRAKFSEVNFGWGEAVYGGAVKGGIGSFLGGTFLVPHKNAKGEEGLILPICLPPEDMKRFVKELNDMLGNQNHPTRSAPSFILSTL*