Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1391950 | YP_009237637.1 | 100.000 | 125 | 0 | 0 | 1 | 125 | 1 | 125 | 1.89e-84 | 252 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1391950 | sp|P27070|RT12_PANGI | 94.400 | 125 | 7 | 0 | 1 | 125 | 1 | 125 | 1.35e-80 | 235 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1391950 | A0A126TGT6 | 100.000 | 125 | 0 | 0 | 1 | 125 | 1 | 125 | 9.02e-85 | 252 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0069510 | Msa1391950 | 0.873344 | 1.581623e-67 | -8.615850e-47 |
| Msa0069520 | Msa1391950 | 0.926744 | 3.076722e-91 | -8.615850e-47 |
| Msa1391950 | Msa1391960 | 0.868214 | 7.711384e-66 | -8.615850e-47 |
| Msa1391950 | Msa1453850 | 0.947541 | 5.504694e-106 | -8.615850e-47 |
| Msa0983360 | Msa1391950 | 0.855939 | 4.506318e-62 | -8.615850e-47 |
| Msa0983370 | Msa1391950 | 0.935108 | 1.423813e-96 | -8.615850e-47 |
| Msa0919980 | Msa1391950 | 0.963613 | 2.669579e-122 | -8.615850e-47 |
| Msa0919990 | Msa1391950 | 0.823952 | 1.049842e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1391950 | MtrunA17_MTg0491661 | 100.000 | 125 | 0 | 0 | 1 | 125 | 1 | 125 | 1.74e-88 | 252 |
| Msa1391950 | MtrunA17_Chr7g0234981 | 66.667 | 81 | 27 | 0 | 43 | 123 | 13 | 93 | 9.76e-35 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1391950 | AT2G07675.1 | 91.200 | 125 | 11 | 0 | 1 | 125 | 1 | 125 | 1.97e-78 | 227 |
| Msa1391950 | ATMG00980.1 | 91.200 | 125 | 11 | 0 | 1 | 125 | 1 | 125 | 1.97e-78 | 227 |
| Msa1391950 | ATCG00905.1 | 66.265 | 83 | 28 | 0 | 41 | 123 | 3 | 85 | 8.53e-36 | 118 |
| Msa1391950 | ATCG01230.1 | 66.265 | 83 | 28 | 0 | 41 | 123 | 3 | 85 | 8.53e-36 | 118 |
Find 32 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCGGGCGTACTCCTTGCTTC+TGG | 0.225224 | tig0021468:+36870 | None:intergenic |
| CAAGGATTTGTTGGGAATTC+CGG | 0.244708 | tig0021468:-36651 | Msa1391950:CDS |
| ATCTTACGTTGAGCTGAATT+TGG | 0.247376 | tig0021468:+36821 | None:intergenic |
| TGATATATTTGCTCACATTC+CGG | 0.302524 | tig0021468:-36771 | Msa1391950:CDS |
| ATCCTCCATTCATATCGATT+TGG | 0.306818 | tig0021468:+36589 | None:intergenic |
| TCCTCCATTCATATCGATTT+GGG | 0.309921 | tig0021468:+36590 | None:intergenic |
| CGGGCGTACTCCTTGCTTCT+GGG | 0.326117 | tig0021468:+36871 | None:intergenic |
| GATATATTTGCTCACATTCC+GGG | 0.354563 | tig0021468:-36770 | Msa1391950:CDS |
| CGAGGAGTCAAGGATTTGTT+GGG | 0.416175 | tig0021468:-36659 | Msa1391950:CDS |
| TCGAGGAGTCAAGGATTTGT+TGG | 0.418048 | tig0021468:-36660 | Msa1391950:CDS |
| AGAAGAGGCAGATCAAAATA+TGG | 0.430997 | tig0021468:-36623 | Msa1391950:CDS |
| CGAATCAATTGATTTAATGT+AGG | 0.458996 | tig0021468:+36950 | None:intergenic |
| CACGGACCGTACTCGAGCTT+CGG | 0.459749 | tig0021468:-36903 | Msa1391950:CDS |
| TCGATTGCTCAATCGTACTT+TGG | 0.467988 | tig0021468:+36796 | None:intergenic |
| GATCTGCCTCTTCTTCGATC+CGG | 0.495631 | tig0021468:+36632 | None:intergenic |
| CTTGACTCCTCGAATACAAT+GGG | 0.496906 | tig0021468:+36670 | None:intergenic |
| CATTCTACGGTCTTAATAAG+AGG | 0.509133 | tig0021468:-36725 | Msa1391950:CDS |
| GGTAGAGTTAAAGATTTGCC+AGG | 0.536394 | tig0021468:-36701 | Msa1391950:CDS |
| TTTGCTCACATTCCGGGCGA+AGG | 0.538531 | tig0021468:-36764 | Msa1391950:CDS |
| ATTGATCCGAAGCTCGAGTA+CGG | 0.542451 | tig0021468:+36897 | None:intergenic |
| GTGAAATCCCATTGTATTCG+AGG | 0.549831 | tig0021468:-36677 | Msa1391950:CDS |
| CCTTGACTCCTCGAATACAA+TGG | 0.552314 | tig0021468:+36669 | None:intergenic |
| CCATTGTATTCGAGGAGTCA+AGG | 0.553703 | tig0021468:-36669 | Msa1391950:CDS |
| GGGCGAAGGTCATAATTCGC+AGG | 0.554524 | tig0021468:-36750 | Msa1391950:CDS |
| TGCGAATTATGACCTTCGCC+CGG | 0.559329 | tig0021468:+36752 | None:intergenic |
| TAATTCGCAGGAACATTCTA+CGG | 0.570921 | tig0021468:-36738 | Msa1391950:CDS |
| TTAAATCAATTGATTCGTCA+TGG | 0.572229 | tig0021468:-36944 | Msa1391950:CDS |
| ATACAATGGGATTTCACACC+TGG | 0.575815 | tig0021468:+36683 | None:intergenic |
| GGGCGTACTCCTTGCTTCTG+GGG | 0.603634 | tig0021468:+36872 | None:intergenic |
| GATCAATGTCCCCAGAAGCA+AGG | 0.611270 | tig0021468:-36881 | Msa1391950:CDS |
| GGAATTCCGGATCGAAGAAG+AGG | 0.614106 | tig0021468:-36638 | Msa1391950:CDS |
| TCTACGGTCTTAATAAGAGG+AGG | 0.670870 | tig0021468:-36722 | Msa1391950:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CGAATCAATTGATTTAATGT+AGG | + | tig0021468:36604-36623 | None:intergenic | 25.0% |
| !!! | TTAAATCAATTGATTCGTCA+TGG | - | tig0021468:36607-36626 | Msa1391950:CDS | 25.0% |
| !!! | TGAGCTGAATTTGGTTTTTT+CGG | + | tig0021468:36724-36743 | None:intergenic | 30.0% |
| TGATATATTTGCTCACATTC+CGG | - | tig0021468:36780-36799 | Msa1391950:CDS | 30.0% | |
| GTCATGGTAGAGAAGAAAAA+CGG | - | tig0021468:36623-36642 | Msa1391950:CDS | 35.0% | |
| ATCTTACGTTGAGCTGAATT+TGG | + | tig0021468:36733-36752 | None:intergenic | 35.0% | |
| GATATATTTGCTCACATTCC+GGG | - | tig0021468:36781-36800 | Msa1391950:CDS | 35.0% | |
| TAATTCGCAGGAACATTCTA+CGG | - | tig0021468:36813-36832 | Msa1391950:CDS | 35.0% | |
| CATTCTACGGTCTTAATAAG+AGG | - | tig0021468:36826-36845 | Msa1391950:CDS | 35.0% | |
| AGAAGAGGCAGATCAAAATA+TGG | - | tig0021468:36928-36947 | Msa1391950:CDS | 35.0% | |
| GTGTTCTCTTAAAAACACGC+GGG | + | tig0021468:36702-36721 | None:intergenic | 40.0% | |
| GGTGTTCTCTTAAAAACACG+CGG | + | tig0021468:36703-36722 | None:intergenic | 40.0% | |
| TCGATTGCTCAATCGTACTT+TGG | + | tig0021468:36758-36777 | None:intergenic | 40.0% | |
| TCTACGGTCTTAATAAGAGG+AGG | - | tig0021468:36829-36848 | Msa1391950:CDS | 40.0% | |
| GGTAGAGTTAAAGATTTGCC+AGG | - | tig0021468:36850-36869 | Msa1391950:CDS | 40.0% | |
| ATACAATGGGATTTCACACC+TGG | + | tig0021468:36871-36890 | None:intergenic | 40.0% | |
| GTGAAATCCCATTGTATTCG+AGG | - | tig0021468:36874-36893 | Msa1391950:CDS | 40.0% | |
| CTTGACTCCTCGAATACAAT+GGG | + | tig0021468:36884-36903 | None:intergenic | 40.0% | |
| !! | CAAGGATTTGTTGGGAATTC+CGG | - | tig0021468:36900-36919 | Msa1391950:CDS | 40.0% |
| TAGAGAAGAAAAACGGCGCA+CGG | - | tig0021468:36630-36649 | Msa1391950:CDS | 45.0% | |
| ATTGATCCGAAGCTCGAGTA+CGG | + | tig0021468:36657-36676 | None:intergenic | 45.0% | |
| CCTTGACTCCTCGAATACAA+TGG | + | tig0021468:36885-36904 | None:intergenic | 45.0% | |
| CCATTGTATTCGAGGAGTCA+AGG | - | tig0021468:36882-36901 | Msa1391950:CDS | 45.0% | |
| TCGAGGAGTCAAGGATTTGT+TGG | - | tig0021468:36891-36910 | Msa1391950:CDS | 45.0% | |
| ! | CGAGGAGTCAAGGATTTGTT+GGG | - | tig0021468:36892-36911 | Msa1391950:CDS | 45.0% |
| GATCAATGTCCCCAGAAGCA+AGG | - | tig0021468:36670-36689 | Msa1391950:CDS | 50.0% | |
| TGCGAATTATGACCTTCGCC+CGG | + | tig0021468:36802-36821 | None:intergenic | 50.0% | |
| GGAATTCCGGATCGAAGAAG+AGG | - | tig0021468:36913-36932 | Msa1391950:CDS | 50.0% | |
| GATCTGCCTCTTCTTCGATC+CGG | + | tig0021468:36922-36941 | None:intergenic | 50.0% | |
| TTTGCTCACATTCCGGGCGA+AGG | - | tig0021468:36787-36806 | Msa1391950:CDS | 55.0% | |
| GGGCGAAGGTCATAATTCGC+AGG | - | tig0021468:36801-36820 | Msa1391950:CDS | 55.0% | |
| CACGGACCGTACTCGAGCTT+CGG | - | tig0021468:36648-36667 | Msa1391950:CDS | 60.0% | |
| GGGCGTACTCCTTGCTTCTG+GGG | + | tig0021468:36682-36701 | None:intergenic | 60.0% | |
| CGGGCGTACTCCTTGCTTCT+GGG | + | tig0021468:36683-36702 | None:intergenic | 60.0% | |
| GCGGGCGTACTCCTTGCTTC+TGG | + | tig0021468:36684-36703 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0021468 | gene | 36598 | 36975 | 36598 | ID=Msa1391950;Name=Msa1391950 |
| tig0021468 | mRNA | 36598 | 36975 | 36598 | ID=Msa1391950-mRNA-1;Parent=Msa1391950;Name=Msa1391950-mRNA-1;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|125 |
| tig0021468 | exon | 36598 | 36975 | 36598 | ID=Msa1391950-mRNA-1:exon:12967;Parent=Msa1391950-mRNA-1 |
| tig0021468 | CDS | 36598 | 36975 | 36598 | ID=Msa1391950-mRNA-1:cds;Parent=Msa1391950-mRNA-1 |
| Gene Sequence |
| Protein sequence |
