AlfalfaGEDB | Msa1453850

M. sativa cultivar zhongmu-4 / Msa1453850

KEGG: K02950
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
PFAM: PF00164.28

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1453850	sp\|P27070\|RT12_PANGI	94.400	125	7	0	1	125	1	125	1.35e-80	235

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1453850	A0A126TGT6	100.000	125	0	0	1	125	1	125	9.02e-85	252

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0069510	Msa1453850	0.805962	1.036052e-49	-8.615850e-47
Msa0069520	Msa1453850	0.873492	1.410290e-67	-8.615850e-47
Msa1391950	Msa1453850	0.947541	5.504694e-106	-8.615850e-47
Msa1391960	Msa1453850	0.800146	1.652836e-48	-8.615850e-47
Msa0983370	Msa1453850	0.944141	3.358986e-103	-8.615850e-47
Msa0919980	Msa1453850	0.920432	1.288884e-87	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1453850	MtrunA17_MTg0491661	100.000	125	0	0	1	125	1	125	1.74e-88	252
Msa1453850	MtrunA17_Chr7g0234981	66.667	81	27	0	43	123	13	93	9.76e-35	115

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
Msa1453850	AT2G07675.1	91.200	125	11	1	125	1	125	1.97e-78	227
Msa1453850	ATMG00980.1	91.200	125	11	1	125	1	125	1.97e-78	227
Msa1453850	ATCG00905.1	66.265	83	28	41	123	3	85	8.53e-36	118
Msa1453850	ATCG01230.1	66.265	83	28	41	123	3	85	8.53e-36	118

Find 32 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCGGGCGTACTCCTTGCTTC+TGG	0.225224	tig0034911:+22491	None:intergenic
CAAGGATTTGTTGGGAATTC+CGG	0.244708	tig0034911:-22272	Msa1453850:CDS
ATCTTACGTTGAGCTGAATT+TGG	0.247376	tig0034911:+22442	None:intergenic
TGATATATTTGCTCACATTC+CGG	0.302524	tig0034911:-22392	Msa1453850:CDS
ATCCTCCATTCATATCGATT+TGG	0.306818	tig0034911:+22210	None:intergenic
TCCTCCATTCATATCGATTT+GGG	0.309921	tig0034911:+22211	None:intergenic
CGGGCGTACTCCTTGCTTCT+GGG	0.326117	tig0034911:+22492	None:intergenic
GATATATTTGCTCACATTCC+GGG	0.354563	tig0034911:-22391	Msa1453850:CDS
CGAGGAGTCAAGGATTTGTT+GGG	0.416175	tig0034911:-22280	Msa1453850:CDS
TCGAGGAGTCAAGGATTTGT+TGG	0.418048	tig0034911:-22281	Msa1453850:CDS
AGAAGAGGCAGATCAAAATA+TGG	0.430997	tig0034911:-22244	Msa1453850:CDS
CGAATCAATTGATTTAATGT+AGG	0.458996	tig0034911:+22571	None:intergenic
CACGGACCGTACTCGAGCTT+CGG	0.459749	tig0034911:-22524	Msa1453850:CDS
TCGATTGCTCAATCGTACTT+TGG	0.467988	tig0034911:+22417	None:intergenic
GATCTGCCTCTTCTTCGATC+CGG	0.495631	tig0034911:+22253	None:intergenic
CTTGACTCCTCGAATACAAT+GGG	0.496906	tig0034911:+22291	None:intergenic
CATTCTACGGTCTTAATAAG+AGG	0.509133	tig0034911:-22346	Msa1453850:CDS
GGTAGAGTTAAAGATTTGCC+AGG	0.536394	tig0034911:-22322	Msa1453850:CDS
TTTGCTCACATTCCGGGCGA+AGG	0.538531	tig0034911:-22385	Msa1453850:CDS
ATTGATCCGAAGCTCGAGTA+CGG	0.542451	tig0034911:+22518	None:intergenic
GTGAAATCCCATTGTATTCG+AGG	0.549831	tig0034911:-22298	Msa1453850:CDS
CCTTGACTCCTCGAATACAA+TGG	0.552314	tig0034911:+22290	None:intergenic
CCATTGTATTCGAGGAGTCA+AGG	0.553703	tig0034911:-22290	Msa1453850:CDS
GGGCGAAGGTCATAATTCGC+AGG	0.554524	tig0034911:-22371	Msa1453850:CDS
TGCGAATTATGACCTTCGCC+CGG	0.559329	tig0034911:+22373	None:intergenic
TAATTCGCAGGAACATTCTA+CGG	0.570921	tig0034911:-22359	Msa1453850:CDS
TTAAATCAATTGATTCGTCA+TGG	0.572229	tig0034911:-22565	Msa1453850:CDS
ATACAATGGGATTTCACACC+TGG	0.575815	tig0034911:+22304	None:intergenic
GGGCGTACTCCTTGCTTCTG+GGG	0.603634	tig0034911:+22493	None:intergenic
GATCAATGTCCCCAGAAGCA+AGG	0.611270	tig0034911:-22502	Msa1453850:CDS
GGAATTCCGGATCGAAGAAG+AGG	0.614106	tig0034911:-22259	Msa1453850:CDS
TCTACGGTCTTAATAAGAGG+AGG	0.670870	tig0034911:-22343	Msa1453850:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CGAATCAATTGATTTAATGT+AGG	+	tig0034911:22225-22244	None:intergenic	25.0%
!!!	TTAAATCAATTGATTCGTCA+TGG	-	tig0034911:22228-22247	Msa1453850:CDS	25.0%
!!!	TGAGCTGAATTTGGTTTTTT+CGG	+	tig0034911:22345-22364	None:intergenic	30.0%
	TGATATATTTGCTCACATTC+CGG	-	tig0034911:22401-22420	Msa1453850:CDS	30.0%
	GTCATGGTAGAGAAGAAAAA+CGG	-	tig0034911:22244-22263	Msa1453850:CDS	35.0%
	ATCTTACGTTGAGCTGAATT+TGG	+	tig0034911:22354-22373	None:intergenic	35.0%
	GATATATTTGCTCACATTCC+GGG	-	tig0034911:22402-22421	Msa1453850:CDS	35.0%
	TAATTCGCAGGAACATTCTA+CGG	-	tig0034911:22434-22453	Msa1453850:CDS	35.0%
	CATTCTACGGTCTTAATAAG+AGG	-	tig0034911:22447-22466	Msa1453850:CDS	35.0%
	AGAAGAGGCAGATCAAAATA+TGG	-	tig0034911:22549-22568	Msa1453850:CDS	35.0%
	GTGTTCTCTTAAAAACACGC+GGG	+	tig0034911:22323-22342	None:intergenic	40.0%
	GGTGTTCTCTTAAAAACACG+CGG	+	tig0034911:22324-22343	None:intergenic	40.0%
	TCGATTGCTCAATCGTACTT+TGG	+	tig0034911:22379-22398	None:intergenic	40.0%
	TCTACGGTCTTAATAAGAGG+AGG	-	tig0034911:22450-22469	Msa1453850:CDS	40.0%
	GGTAGAGTTAAAGATTTGCC+AGG	-	tig0034911:22471-22490	Msa1453850:CDS	40.0%
	ATACAATGGGATTTCACACC+TGG	+	tig0034911:22492-22511	None:intergenic	40.0%
	GTGAAATCCCATTGTATTCG+AGG	-	tig0034911:22495-22514	Msa1453850:CDS	40.0%
	CTTGACTCCTCGAATACAAT+GGG	+	tig0034911:22505-22524	None:intergenic	40.0%
!!	CAAGGATTTGTTGGGAATTC+CGG	-	tig0034911:22521-22540	Msa1453850:CDS	40.0%
	TAGAGAAGAAAAACGGCGCA+CGG	-	tig0034911:22251-22270	Msa1453850:CDS	45.0%
	ATTGATCCGAAGCTCGAGTA+CGG	+	tig0034911:22278-22297	None:intergenic	45.0%
	CCTTGACTCCTCGAATACAA+TGG	+	tig0034911:22506-22525	None:intergenic	45.0%
	CCATTGTATTCGAGGAGTCA+AGG	-	tig0034911:22503-22522	Msa1453850:CDS	45.0%
	TCGAGGAGTCAAGGATTTGT+TGG	-	tig0034911:22512-22531	Msa1453850:CDS	45.0%
!	CGAGGAGTCAAGGATTTGTT+GGG	-	tig0034911:22513-22532	Msa1453850:CDS	45.0%
	GATCAATGTCCCCAGAAGCA+AGG	-	tig0034911:22291-22310	Msa1453850:CDS	50.0%
	TGCGAATTATGACCTTCGCC+CGG	+	tig0034911:22423-22442	None:intergenic	50.0%
	GGAATTCCGGATCGAAGAAG+AGG	-	tig0034911:22534-22553	Msa1453850:CDS	50.0%
	GATCTGCCTCTTCTTCGATC+CGG	+	tig0034911:22543-22562	None:intergenic	50.0%
	TTTGCTCACATTCCGGGCGA+AGG	-	tig0034911:22408-22427	Msa1453850:CDS	55.0%
	GGGCGAAGGTCATAATTCGC+AGG	-	tig0034911:22422-22441	Msa1453850:CDS	55.0%
	CACGGACCGTACTCGAGCTT+CGG	-	tig0034911:22269-22288	Msa1453850:CDS	60.0%
	GGGCGTACTCCTTGCTTCTG+GGG	+	tig0034911:22303-22322	None:intergenic	60.0%
	CGGGCGTACTCCTTGCTTCT+GGG	+	tig0034911:22304-22323	None:intergenic	60.0%
	GCGGGCGTACTCCTTGCTTC+TGG	+	tig0034911:22305-22324	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
tig0034911	gene	22219	22596	22219	ID=Msa1453850;Name=Msa1453850
tig0034911	mRNA	22219	22596	22219	ID=Msa1453850-mRNA-1;Parent=Msa1453850;Name=Msa1453850-mRNA-1;_AED=0.17;_eAED=0.17;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|125
tig0034911	exon	22219	22596	22219	ID=Msa1453850-mRNA-1:exon:19349;Parent=Msa1453850-mRNA-1
tig0034911	CDS	22219	22596	22219	ID=Msa1453850-mRNA-1:cds;Parent=Msa1453850-mRNA-1

Gene Sequence	>Msa1453850 ATGCCTACATTAAATCAATTGATTCGTCATGGTAGAGAAGAAAAACGGCGCACGGACCGTACTCGAGCTTCGGATCAATGTCCCCAGAAGCAAGGAGTACGCCCGCGTGTTTTTAAGAGAACACCGAAAAAACCAAATTCAGCTCAACGTAAGATAGCCAAAGTACGATTGAGCAATCGACATGATATATTTGCTCACATTCCGGGCGAAGGTCATAATTCGCAGGAACATTCTACGGTCTTAATAAGAGGAGGTAGAGTTAAAGATTTGCCAGGTGTGAAATCCCATTGTATTCGAGGAGTCAAGGATTTGTTGGGAATTCCGGATCGAAGAAGAGGCAGATCAAAATATGGTGCAGAAAAACCCAAATCGATATGA
Protein sequence	>Msa1453850 MPTLNQLIRHGREEKRRTDRTRASDQCPQKQGVRPRVFKRTPKKPNSAQRKIAKVRLSNRHDIFAHIPGEGHNSQEHSTVLIRGGRVKDLPGVKSHCIRGVKDLLGIPDRRRGRSKYGAEKPKSI*