AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1393610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1393610 MtrunA17_Chr8g0388501 96.894 322 9 1 1 321 1 322 0.0 639
Msa1393610 MtrunA17_Chr3g0120061 60.059 338 111 5 1 321 1 331 6.92e-131 376
Msa1393610 MtrunA17_Chr7g0225451 51.402 321 113 8 6 308 2 297 9.24e-99 293
Msa1393610 MtrunA17_Chr7g0243491 48.305 118 57 2 168 282 20 136 5.56e-26 106
Msa1393610 MtrunA17_Chr3g0081541 27.451 306 190 7 34 321 86 377 1.01e-19 89.7
Msa1393610 MtrunA17_Chr4g0043691 37.121 132 77 2 178 307 17 144 4.82e-19 88.2
Msa1393610 MtrunA17_Chr2g0311141 34.437 151 88 3 163 306 93 239 5.14e-19 87.4
Msa1393610 MtrunA17_Chr3g0131611 36.567 134 70 3 178 306 14 137 1.09e-17 83.6
Msa1393610 MtrunA17_Chr3g0081571 34.815 135 79 3 174 306 306 433 2.98e-16 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1393610 AT5G42050.1 59.155 355 93 9 1 310 1 348 1.68e-121 353
Msa1393610 AT3G27090.1 52.805 303 126 6 10 308 3 292 7.43e-98 291
Msa1393610 AT3G27090.2 52.805 303 126 6 10 308 3 292 7.43e-98 291
Msa1393610 AT3G27090.3 52.805 303 126 6 10 308 3 292 7.43e-98 291
Msa1393610 AT5G01660.3 41.667 120 67 2 178 295 18 136 4.94e-25 105
Msa1393610 AT5G01660.2 41.667 120 67 2 178 295 18 136 7.61e-25 105
Msa1393610 AT5G01660.1 41.667 120 67 2 178 295 18 136 7.61e-25 105
Msa1393610 AT3G11000.1 42.424 132 73 2 178 307 11 141 2.60e-24 103
Msa1393610 AT3G11000.2 42.424 132 73 2 178 307 130 260 6.87e-24 102
Msa1393610 AT5G61910.1 37.500 136 77 2 172 306 55 183 2.04e-22 98.2
Msa1393610 AT5G61910.2 37.500 136 77 2 172 306 55 183 2.04e-22 98.2
Msa1393610 AT5G61910.3 37.500 136 77 2 172 306 59 187 2.12e-22 98.2
Msa1393610 AT5G61910.5 37.500 136 77 2 172 306 55 183 2.61e-22 97.8
Msa1393610 AT5G61910.4 37.500 136 77 2 172 306 55 183 6.23e-21 94.4
Msa1393610 AT2G35140.6 34.722 144 88 3 180 321 49 188 9.34e-19 87.4
Msa1393610 AT2G35140.5 34.722 144 88 3 180 321 23 162 9.51e-19 87.4
Msa1393610 AT2G35140.1 34.722 144 88 3 180 321 23 162 9.51e-19 87.4
Msa1393610 AT2G35140.4 34.722 144 88 3 180 321 23 162 9.51e-19 87.4
Msa1393610 AT2G35140.2 34.722 144 88 3 180 321 23 162 9.51e-19 87.4
Msa1393610 AT2G35140.3 34.722 144 88 3 180 321 23 162 9.51e-19 87.4
Msa1393610 AT2G32910.1 29.536 237 141 9 78 306 224 442 3.43e-18 85.9

Find 85 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGGTTGTGTCAGCATAAA+AGG 0.132335 tig0021743:-103226 Msa1393610:exon
ACTTGATATGTTTCAACTTC+TGG 0.192127 tig0021743:-102829 Msa1393610:three_prime_UTR
CGGTTGAATGGTTCATTATA+GGG 0.238859 tig0021743:-102894 Msa1393610:three_prime_UTR
ACACTAGATAATTAGAGATT+TGG 0.253892 tig0021743:-103141 Msa1393610:three_prime_UTR
CCTAACTGAATCCCTGTATC+TGG 0.267819 tig0021743:+104066 None:intergenic
TGGTATAGTTGTAAAGAAAA+AGG 0.278735 tig0021743:+104023 None:intergenic
GTTGGTGTTTCTCCTATTAA+TGG 0.279410 tig0021743:-105520 Msa1393610:CDS
TTATTAAAACCTCCATTAAT+AGG 0.287108 tig0021743:+105508 None:intergenic
ACGGTTGAATGGTTCATTAT+AGG 0.296900 tig0021743:-102895 Msa1393610:three_prime_UTR
TATGCAAAGCTGCCAATTTA+CGG 0.297962 tig0021743:-102914 Msa1393610:three_prime_UTR
TGAACCATTCAACCGTAAAT+TGG 0.312036 tig0021743:+102902 None:intergenic
ATTTATTCAAAGCCTTCTTA+TGG 0.312416 tig0021743:-105481 Msa1393610:CDS
TCAGTTAGGGCCATTACTCC+TGG 0.331278 tig0021743:-104052 Msa1393610:CDS
ATTAGGGTTTGAATTCTTCT+TGG 0.332710 tig0021743:+105390 None:intergenic
CACTAGATAATTAGAGATTT+GGG 0.337887 tig0021743:-103140 Msa1393610:three_prime_UTR
TATCTCTTAATGATTCAATC+TGG 0.342706 tig0021743:-105699 Msa1393610:CDS
AGATTGAATCATTAAGAGAT+AGG 0.365054 tig0021743:+105701 None:intergenic
TAGGTTTGCCTCCCAGATAC+AGG 0.367382 tig0021743:-104078 Msa1393610:CDS
CCAGATACAGGGATTCAGTT+AGG 0.380760 tig0021743:-104066 Msa1393610:CDS
CCACCAATGGTTTCATTCCT+AGG 0.384980 tig0021743:+105265 None:intergenic
TTCTTCTTGGAAGATTTAGA+TGG 0.386595 tig0021743:+105403 None:intergenic
AACAACAACTCTGTTTCTCT+TGG 0.386776 tig0021743:-105601 Msa1393610:CDS
TTATTGAAATGATCAGAAAC+AGG 0.392314 tig0021743:+105571 None:intergenic
TTTGTTTAGGCTGCAAGTTT+TGG 0.392417 tig0021743:-103770 Msa1393610:intron
CAAATTACTACAAAGAATAA+AGG 0.401147 tig0021743:-103192 Msa1393610:three_prime_UTR
TCTGATCATTTCAATAATCA+AGG 0.410681 tig0021743:-105565 Msa1393610:CDS
AATCTCAAAAGGCAACTCTT+TGG 0.419984 tig0021743:-105205 Msa1393610:intron
CCATCATAGTGGTGAAGAAC+TGG 0.427123 tig0021743:+103435 None:intergenic
TGCTTCCAACGACACTTTCA+AGG 0.433408 tig0021743:-103244 Msa1393610:CDS
ATCTCTTAATGATTCAATCT+GGG 0.445296 tig0021743:-105698 Msa1393610:CDS
TTAAAATTCCCACCATAAGA+AGG 0.446938 tig0021743:+105469 None:intergenic
AAATATTGATCCAACGGCTT+GGG 0.453134 tig0021743:-103741 Msa1393610:CDS
CACTAGAAGAGGATTCATTC+AGG 0.455058 tig0021743:-103458 Msa1393610:CDS
CGAATCTCGTTTCCCTGCTC+AGG 0.457693 tig0021743:-103699 Msa1393610:intron
TACAGGTAAGAGTGCTTACT+AGG 0.458108 tig0021743:-103494 Msa1393610:intron
GCTGCCAATTTACGGTTGAA+TGG 0.466789 tig0021743:-102906 Msa1393610:three_prime_UTR
TCTCTTGTCAGCAGCCGCCT+TGG 0.468255 tig0021743:+105318 None:intergenic
TTCTTGTCCTCCCAAGCCGT+TGG 0.480092 tig0021743:+103731 None:intergenic
CAGTTAGGGCCATTACTCCT+GGG 0.491247 tig0021743:-104051 Msa1393610:CDS
AATGAAAATGATACCTGAGC+AGG 0.497887 tig0021743:+103686 None:intergenic
AAGGTTGTGTCAGCATAAAA+GGG 0.500936 tig0021743:-103225 Msa1393610:exon
AGCTCTATAACACAATATAT+AGG 0.508792 tig0021743:+103041 None:intergenic
GTTGTAGGCATTGTCTCTGC+TGG 0.511637 tig0021743:-103286 Msa1393610:intron
CAGATACAGGGATTCAGTTA+GGG 0.515717 tig0021743:-104065 Msa1393610:CDS
CCAGTTCTTCACCACTATGA+TGG 0.516230 tig0021743:-103435 Msa1393610:CDS
TGGGAGGACAAGAAGTGTGC+TGG 0.518213 tig0021743:-103722 Msa1393610:CDS
TATTCAAAGCCTTCTTATGG+TGG 0.524188 tig0021743:-105478 Msa1393610:CDS
CAAATATTGATCCAACGGCT+TGG 0.524783 tig0021743:-103742 Msa1393610:CDS
CTAACTGAATCCCTGTATCT+GGG 0.538745 tig0021743:+104067 None:intergenic
AAGGGGCAACCCAGGAGTAA+TGG 0.541588 tig0021743:+104042 None:intergenic
ATTCAAAGCCTTCTTATGGT+GGG 0.541890 tig0021743:-105477 Msa1393610:CDS
TATTGATCCAACGGCTTGGG+AGG 0.545418 tig0021743:-103738 Msa1393610:CDS
GGTGTTTCTCCTATTAATGG+AGG 0.546103 tig0021743:-105517 Msa1393610:CDS
CACAACCTTGAAAGTGTCGT+TGG 0.548994 tig0021743:+103239 None:intergenic
AGTAACATGAATGTTACTAA+AGG 0.551944 tig0021743:-105445 Msa1393610:CDS
CTGAATGAATCCTCTTCTAG+TGG 0.554234 tig0021743:+103459 None:intergenic
CCATGGCTGAGAATCTCAAA+AGG 0.555325 tig0021743:-105216 Msa1393610:CDS
AGGTTTGCCTCCCAGATACA+GGG 0.571707 tig0021743:-104077 Msa1393610:CDS
CACCAGCAGAGTCTCTTCCT+AGG 0.573046 tig0021743:-105282 Msa1393610:CDS
GGGGAGAACAATAACAGTGA+TGG 0.573073 tig0021743:-105358 Msa1393610:CDS
CCTAGGAATGAAACCATTGG+TGG 0.576885 tig0021743:-105265 Msa1393610:CDS
TTCAGCTCAAGACGGAACTT+AGG 0.577473 tig0021743:+103411 None:intergenic
GGAACTTAGGTCCATCATAG+TGG 0.580135 tig0021743:+103424 None:intergenic
TATACCACTCACCAACTCCA+TGG 0.580365 tig0021743:-104007 Msa1393610:CDS
CTTCCTAGGAATGAAACCAT+TGG 0.584075 tig0021743:-105268 Msa1393610:CDS
TTCCTAGGAAGAGACTCTGC+TGG 0.585025 tig0021743:+105280 None:intergenic
AACATGTGAGCCACTAGAAG+AGG 0.589083 tig0021743:-103469 Msa1393610:CDS
ATGAAAATGATACCTGAGCA+GGG 0.598544 tig0021743:+103687 None:intergenic
GGTTTGCTAGATTTGATGTG+TGG 0.599698 tig0021743:+105722 None:intergenic
TCTCTTAATGATTCAATCTG+GGG 0.606044 tig0021743:-105697 Msa1393610:CDS
TGGAACAAATATTGATCCAA+CGG 0.610123 tig0021743:-103747 Msa1393610:CDS
AAATAAGAACAAGGATTCCA+AGG 0.613003 tig0021743:-105335 Msa1393610:CDS
CAGTGATGGAAATAAGAACA+AGG 0.622810 tig0021743:-105344 Msa1393610:CDS
AACTCCATGGAGTTGGTGAG+TGG 0.632771 tig0021743:+104003 None:intergenic
TATTGAAATGATCAGAAACA+GGG 0.638940 tig0021743:+105572 None:intergenic
CTAGGAATGAAACCATTGGT+GGG 0.642582 tig0021743:-105264 Msa1393610:CDS
GACAAAGATATACCCACCAA+TGG 0.644157 tig0021743:+105252 None:intergenic
CTAGGAAGAGACTCTGCTGG+TGG 0.665578 tig0021743:+105283 None:intergenic
CAGGCACATTCAGCTCAAGA+CGG 0.668323 tig0021743:+103403 None:intergenic
TCTTGAGCTGAATGTGCCTG+AGG 0.674355 tig0021743:-103400 Msa1393610:intron
TTACATATACACATACACAT+AGG 0.682964 tig0021743:-105832 Msa1393610:five_prime_UTR
TGTCTGTAACAATGATACCA+TGG 0.683554 tig0021743:-105233 Msa1393610:CDS
ATTGAAATGATCAGAAACAG+GGG 0.684933 tig0021743:+105573 None:intergenic
ACTGAATCCCTGTATCTGGG+AGG 0.733702 tig0021743:+104070 None:intergenic
TAAGAACAAGGATTCCAAGG+CGG 0.748891 tig0021743:-105332 Msa1393610:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTGACAAAATTTTTTTA+AGG - tig0021743:103720-103739 Msa1393610:CDS 10.0%
!!! AAAATTTTTTTAAGGTAAAA+AGG - tig0021743:103728-103747 Msa1393610:CDS 10.0%
!! TAAACAAAATTAAAAAAATG+GGG + tig0021743:104066-104085 None:intergenic 10.0%
!! ATAATATATTTACTATAGAT+TGG - tig0021743:104515-104534 Msa1393610:intron 10.0%
!! TGAAATAATTAAAAAAAAAG+GGG + tig0021743:105423-105442 None:intergenic 10.0%
!!! AATTGTTTTTTACATATATA+CGG + tig0021743:105683-105702 None:intergenic 10.0%
!!! ATTATTTCCCTTTTTTTTTT+TGG - tig0021743:103665-103684 Msa1393610:intron 15.0%
!!! TGAGTTTTTATTTGAAAATT+CGG - tig0021743:103895-103914 Msa1393610:intron 15.0%
!!! TATAAGTTAAAGTGTAATTT+TGG - tig0021743:104094-104113 Msa1393610:intron 15.0%
!!! ATAAGTTAAAGTGTAATTTT+GGG - tig0021743:104095-104114 Msa1393610:intron 15.0%
!! GAAATAATTAAAAAAAAAGG+GGG + tig0021743:105422-105441 None:intergenic 15.0%
!!! TTTGTTTTTATATATAGCTA+CGG - tig0021743:105642-105661 Msa1393610:CDS 15.0%
!!! TTTCAAGTTTTGTGAAAAAA+TGG - tig0021743:102959-102978 Msa1393610:three_prime_UTR 20.0%
!!! AAAAAGATTGTTGATTGTTA+TGG + tig0021743:102992-103011 None:intergenic 20.0%
!!! ATAACAATCAACAATCTTTT+TGG - tig0021743:102991-103010 Msa1393610:three_prime_UTR 20.0%
!!! AAAATTTCTCCTTTGATTTT+TGG + tig0021743:103105-103124 None:intergenic 20.0%
!!! AGAAATTTTGATACAAAACT+TGG - tig0021743:103116-103135 Msa1393610:three_prime_UTR 20.0%
!! TTATTAAAACCTCCATTAAT+AGG + tig0021743:103254-103273 None:intergenic 20.0%
!!! TATTAATGGAGGTTTTAATA+AGG - tig0021743:103253-103272 Msa1393610:CDS 20.0%
!!! ATTAATGGAGGTTTTAATAA+GGG - tig0021743:103254-103273 Msa1393610:CDS 20.0%
!! ATAATTGAAGGAAACAATTT+AGG + tig0021743:103596-103615 None:intergenic 20.0%
!! AATAAGAATTGCATAATTGA+AGG + tig0021743:103608-103627 None:intergenic 20.0%
!! AATCGAACCAAAAAAAAAAA+GGG + tig0021743:103675-103694 None:intergenic 20.0%
!! TAATCGAACCAAAAAAAAAA+AGG + tig0021743:103676-103695 None:intergenic 20.0%
!! TTATTTGAAAATTCGGTATT+CGG - tig0021743:103902-103921 Msa1393610:intron 20.0%
!!! ATTTTACTTCTGTCTTAAAT+GGG + tig0021743:103963-103982 None:intergenic 20.0%
!!! AATTTTACTTCTGTCTTAAA+TGG + tig0021743:103964-103983 None:intergenic 20.0%
!!! ATTTTCGAAGTAATTTATTC+CGG + tig0021743:104153-104172 None:intergenic 20.0%
!! GTAAATATATTATGCTTCTA+TGG + tig0021743:104508-104527 None:intergenic 20.0%
!! AAAAACATAATTGATACTGA+AGG + tig0021743:104636-104655 None:intergenic 20.0%
!!! CACAATTTTAATGGATAATT+CGG + tig0021743:104859-104878 None:intergenic 20.0%
!! CAAAATGTATTCAAATTATG+AGG + tig0021743:104912-104931 None:intergenic 20.0%
!!! CTCATAATTTGAATACATTT+TGG - tig0021743:104910-104929 Msa1393610:intron 20.0%
!! TCTTTAACATTGATAAATAC+AGG - tig0021743:105248-105267 Msa1393610:CDS 20.0%
!! CAAATTACTACAAAGAATAA+AGG - tig0021743:105567-105586 Msa1393610:CDS 20.0%
!! TATGTAAAAAACAATTTGAG+AGG - tig0021743:105687-105706 Msa1393610:CDS 20.0%
! TTACATATACACATACACAT+AGG - tig0021743:102927-102946 Msa1393610:three_prime_UTR 25.0%
! AGATTGAATCATTAAGAGAT+AGG + tig0021743:103061-103080 None:intergenic 25.0%
! TATCTCTTAATGATTCAATC+TGG - tig0021743:103060-103079 Msa1393610:three_prime_UTR 25.0%
! ATCTCTTAATGATTCAATCT+GGG - tig0021743:103061-103080 Msa1393610:three_prime_UTR 25.0%
!!! AATTTTGATACAAAACTTGG+TGG - tig0021743:103119-103138 Msa1393610:three_prime_UTR 25.0%
! TATTGAAATGATCAGAAACA+GGG + tig0021743:103190-103209 None:intergenic 25.0%
! TTATTGAAATGATCAGAAAC+AGG + tig0021743:103191-103210 None:intergenic 25.0%
! TCTGATCATTTCAATAATCA+AGG - tig0021743:103194-103213 Msa1393610:three_prime_UTR 25.0%
!!! TCTTTTTTCTCTTTTGATGT+TGG - tig0021743:103221-103240 Msa1393610:exon 25.0%
! ATTTATTCAAAGCCTTCTTA+TGG - tig0021743:103278-103297 Msa1393610:CDS 25.0%
! AGTAACATGAATGTTACTAA+AGG - tig0021743:103314-103333 Msa1393610:intron 25.0%
! TCAAACCCTAATAAAAAACA+AGG - tig0021743:103380-103399 Msa1393610:intron 25.0%
! CAAACCCTAATAAAAAACAA+GGG - tig0021743:103381-103400 Msa1393610:intron 25.0%
! AAACCCTAATAAAAAACAAG+GGG - tig0021743:103382-103401 Msa1393610:intron 25.0%
!! TTTTCGAAGTAATTTATTCC+GGG + tig0021743:104152-104171 None:intergenic 25.0%
!!! TTTATTTTGGTTACCATGTA+TGG - tig0021743:104396-104415 Msa1393610:intron 25.0%
!!! TTATTTTGGTTACCATGTAT+GGG - tig0021743:104397-104416 Msa1393610:intron 25.0%
! AACAATAACTGCAAAATTTC+AGG + tig0021743:104479-104498 None:intergenic 25.0%
! AATGTCAATTGCAATATTTG+AGG - tig0021743:104579-104598 Msa1393610:intron 25.0%
!! TTGTGAATGTTTGTGTTTTT+AGG - tig0021743:104662-104681 Msa1393610:intron 25.0%
! GTATAGTTGTAAAGAAAAAG+GGG + tig0021743:104737-104756 None:intergenic 25.0%
! GGTATAGTTGTAAAGAAAAA+GGG + tig0021743:104738-104757 None:intergenic 25.0%
! TGGTATAGTTGTAAAGAAAA+AGG + tig0021743:104739-104758 None:intergenic 25.0%
! ATTTATGAGACTGTTAGTTA+GGG - tig0021743:104830-104849 Msa1393610:intron 25.0%
! ATCCATTAAAATTGTGCATT+AGG - tig0021743:104863-104882 Msa1393610:intron 25.0%
!! TAAACTGTGCTTTCTTTTAT+GGG - tig0021743:105451-105470 Msa1393610:CDS 25.0%
! ACACTAGATAATTAGAGATT+TGG - tig0021743:105618-105637 Msa1393610:CDS 25.0%
! CACTAGATAATTAGAGATTT+GGG - tig0021743:105619-105638 Msa1393610:CDS 25.0%
! AGCTCTATAACACAATATAT+AGG + tig0021743:105721-105740 None:intergenic 25.0%
!! TGTCCAATTTTTCTTTTGTT+TGG - tig0021743:105753-105772 Msa1393610:CDS 25.0%
!!! GTCCAATTTTTCTTTTGTTT+GGG - tig0021743:105754-105773 Msa1393610:CDS 25.0%
! ATCCCAAACAAAAGAAAAAT+TGG + tig0021743:105759-105778 None:intergenic 25.0%
!! GTATTGAACTATTTTGCTTT+TGG - tig0021743:105818-105837 Msa1393610:five_prime_UTR 25.0%
TCTCTTAATGATTCAATCTG+GGG - tig0021743:103062-103081 Msa1393610:three_prime_UTR 30.0%
!! ATTTCTCCTTTGATTTTTGG+AGG + tig0021743:103102-103121 None:intergenic 30.0%
ATTGAAATGATCAGAAACAG+GGG + tig0021743:103189-103208 None:intergenic 30.0%
TTAAAATTCCCACCATAAGA+AGG + tig0021743:103293-103312 None:intergenic 30.0%
TTCTTCTTGGAAGATTTAGA+TGG + tig0021743:103359-103378 None:intergenic 30.0%
ATTAGGGTTTGAATTCTTCT+TGG + tig0021743:103372-103391 None:intergenic 30.0%
!!! TCTCCCCTTGTTTTTTATTA+GGG + tig0021743:103388-103407 None:intergenic 30.0%
!!! TTCTCCCCTTGTTTTTTATT+AGG + tig0021743:103389-103408 None:intergenic 30.0%
AAATAAGAACAAGGATTCCA+AGG - tig0021743:103424-103443 Msa1393610:CDS 30.0%
GGAAACAATTTAGGAAAGAA+AGG + tig0021743:103587-103606 None:intergenic 30.0%
GTTTAGCTACTCGAATAATA+TGG - tig0021743:104117-104136 Msa1393610:intron 30.0%
ATAATATGGATTTAGCTACC+CGG - tig0021743:104131-104150 Msa1393610:intron 30.0%
!! TTCTCTATGTACTTGCTATA+GGG + tig0021743:104252-104271 None:intergenic 30.0%
!!! GTCTATCTGTAGCTTTATTT+TGG - tig0021743:104383-104402 Msa1393610:intron 30.0%
!!! TATTTTGGTTACCATGTATG+GGG - tig0021743:104398-104417 Msa1393610:intron 30.0%
TGATATCAATATCACTGTAC+TGG - tig0021743:104421-104440 Msa1393610:intron 30.0%
ACAGGTTATAATCCCTTATA+TGG + tig0021743:104549-104568 None:intergenic 30.0%
TTGACATTGAATTGAAGAAC+AGG + tig0021743:104567-104586 None:intergenic 30.0%
!!! TTTTTGAGGTATGTGTTACT+TGG - tig0021743:104777-104796 Msa1393610:intron 30.0%
ACAGTCTCATAAATGTGATT+AGG + tig0021743:104824-104843 None:intergenic 30.0%
CATTTATGAGACTGTTAGTT+AGG - tig0021743:104829-104848 Msa1393610:intron 30.0%
! TGCCTAATGCACAATTTTAA+TGG + tig0021743:104868-104887 None:intergenic 30.0%
ATGTATTCAAATTATGAGGC+TGG + tig0021743:104908-104927 None:intergenic 30.0%
TGGAACAAATATTGATCCAA+CGG - tig0021743:105012-105031 Msa1393610:intron 30.0%
! CATTTTCATTCCAACCTTTA+CGG - tig0021743:105086-105105 Msa1393610:intron 30.0%
!!! TGTTTTGATCTTCTAGTTTG+TGG - tig0021743:105209-105228 Msa1393610:CDS 30.0%
! CTAAACTGTGCTTTCTTTTA+TGG - tig0021743:105450-105469 Msa1393610:CDS 30.0%
ACTTGATATGTTTCAACTTC+TGG - tig0021743:105930-105949 Msa1393610:five_prime_UTR 30.0%
AACAGAGAACAAAAAGCAGT+TGG + tig0021743:102890-102909 None:intergenic 35.0%
ACTCATCCTCCAAAAATCAA+AGG - tig0021743:103093-103112 Msa1393610:three_prime_UTR 35.0%
! AACAACAACTCTGTTTCTCT+TGG - tig0021743:103158-103177 Msa1393610:three_prime_UTR 35.0%
! GTTGGTGTTTCTCCTATTAA+TGG - tig0021743:103239-103258 Msa1393610:CDS 35.0%
TATTCAAAGCCTTCTTATGG+TGG - tig0021743:103281-103300 Msa1393610:CDS 35.0%
ATTCAAAGCCTTCTTATGGT+GGG - tig0021743:103282-103301 Msa1393610:CDS 35.0%
! CAGTGATGGAAATAAGAACA+AGG - tig0021743:103415-103434 Msa1393610:CDS 35.0%
TGTCTGTAACAATGATACCA+TGG - tig0021743:103526-103545 Msa1393610:intron 35.0%
AATCTCAAAAGGCAACTCTT+TGG - tig0021743:103554-103573 Msa1393610:intron 35.0%
!! GTTCTCTATGTACTTGCTAT+AGG + tig0021743:104253-104272 None:intergenic 35.0%
! AGCTAGCCAAATTTTGTTGA+GGG + tig0021743:104331-104350 None:intergenic 35.0%
! AAGCTAGCCAAATTTTGTTG+AGG + tig0021743:104332-104351 None:intergenic 35.0%
AATCAGATAACATATACCCC+CGG + tig0021743:104363-104382 None:intergenic 35.0%
TATTGATATCACCCCATACA+TGG + tig0021743:104412-104431 None:intergenic 35.0%
ATTGGTGTGTTAGCCATATA+AGG - tig0021743:104533-104552 Msa1393610:intron 35.0%
TTGGTGTGTTAGCCATATAA+GGG - tig0021743:104534-104553 Msa1393610:intron 35.0%
TAATGTTACGTCGTAACATG+TGG - tig0021743:104954-104973 Msa1393610:intron 35.0%
! TTTGTTTAGGCTGCAAGTTT+TGG - tig0021743:104989-105008 Msa1393610:intron 35.0%
AAATATTGATCCAACGGCTT+GGG - tig0021743:105018-105037 Msa1393610:intron 35.0%
ATGAAAATGATACCTGAGCA+GGG + tig0021743:105075-105094 None:intergenic 35.0%
AATGAAAATGATACCTGAGC+AGG + tig0021743:105076-105095 None:intergenic 35.0%
!! TGCTTTCTTTTATGGGTTGT+AGG - tig0021743:105458-105477 Msa1393610:CDS 35.0%
AAGGTTGTGTCAGCATAAAA+GGG - tig0021743:105534-105553 Msa1393610:CDS 35.0%
TATGCAAAGCTGCCAATTTA+CGG - tig0021743:105845-105864 Msa1393610:five_prime_UTR 35.0%
TGAACCATTCAACCGTAAAT+TGG + tig0021743:105860-105879 None:intergenic 35.0%
ACGGTTGAATGGTTCATTAT+AGG - tig0021743:105864-105883 Msa1393610:five_prime_UTR 35.0%
CGGTTGAATGGTTCATTATA+GGG - tig0021743:105865-105884 Msa1393610:five_prime_UTR 35.0%
!! GGTTTGCTAGATTTGATGTG+TGG + tig0021743:103040-103059 None:intergenic 40.0%
GGTGTTTCTCCTATTAATGG+AGG - tig0021743:103242-103261 Msa1393610:CDS 40.0%
TAAGAACAAGGATTCCAAGG+CGG - tig0021743:103427-103446 Msa1393610:CDS 40.0%
CTTCCTAGGAATGAAACCAT+TGG - tig0021743:103491-103510 Msa1393610:CDS 40.0%
CTAGGAATGAAACCATTGGT+GGG - tig0021743:103495-103514 Msa1393610:intron 40.0%
GACAAAGATATACCCACCAA+TGG + tig0021743:103510-103529 None:intergenic 40.0%
AATTGACACGAGTGTATGAG+TGG - tig0021743:104005-104024 Msa1393610:CDS 40.0%
! TGAAGCATAGTCTTAGCTTG+CGG - tig0021743:104028-104047 Msa1393610:CDS 40.0%
TTAAAAAAATGGGGCAGCCA+AGG + tig0021743:104057-104076 None:intergenic 40.0%
! CTAGCCAAATTTTGTTGAGG+GGG + tig0021743:104329-104348 None:intergenic 40.0%
! GCTAGCCAAATTTTGTTGAG+GGG + tig0021743:104330-104349 None:intergenic 40.0%
CTAACTGAATCCCTGTATCT+GGG + tig0021743:104695-104714 None:intergenic 40.0%
CAGATACAGGGATTCAGTTA+GGG - tig0021743:104694-104713 Msa1393610:intron 40.0%
CACATACCTCAAAAACTCCA+TGG + tig0021743:104772-104791 None:intergenic 40.0%
CAAATATTGATCCAACGGCT+TGG - tig0021743:105017-105036 Msa1393610:intron 40.0%
CATACTAAAGCCGTAAAGGT+TGG + tig0021743:105099-105118 None:intergenic 40.0%
! CAACCTTTACGGCTTTAGTA+TGG - tig0021743:105097-105116 Msa1393610:intron 40.0%
TGTCTCGTCTCTACTCTTTA+CGG + tig0021743:105131-105150 None:intergenic 40.0%
GACGAGACAGTAGATTTAGA+CGG - tig0021743:105142-105161 Msa1393610:intron 40.0%
!! TACAGGTAAGAGTGCTTACT+AGG - tig0021743:105265-105284 Msa1393610:CDS 40.0%
CTGAATGAATCCTCTTCTAG+TGG + tig0021743:105303-105322 None:intergenic 40.0%
CACTAGAAGAGGATTCATTC+AGG - tig0021743:105301-105320 Msa1393610:CDS 40.0%
AGTGAACGATCAAATACCTC+AGG + tig0021743:105378-105397 None:intergenic 40.0%
CACTTGCGCGTATTGAATTA+TGG - tig0021743:105394-105413 Msa1393610:CDS 40.0%
CAAGGTTGTGTCAGCATAAA+AGG - tig0021743:105533-105552 Msa1393610:CDS 40.0%
! CAAAAAGCAGTTGGTGTGTG+TGG + tig0021743:102881-102900 None:intergenic 45.0%
GGGGAGAACAATAACAGTGA+TGG - tig0021743:103401-103420 Msa1393610:CDS 45.0%
CCACCAATGGTTTCATTCCT+AGG + tig0021743:103497-103516 None:intergenic 45.0%
CCTAGGAATGAAACCATTGG+TGG - tig0021743:103494-103513 Msa1393610:intron 45.0%
!!! CCTTTTGAGATTCTCAGCCA+TGG + tig0021743:103546-103565 None:intergenic 45.0%
CCATGGCTGAGAATCTCAAA+AGG - tig0021743:103543-103562 Msa1393610:intron 45.0%
TGTCTTAAATGGGCCCTCAA+GGG + tig0021743:103953-103972 None:intergenic 45.0%
TTGGCCCCCTCAACAAAATT+TGG - tig0021743:104322-104341 Msa1393610:intron 45.0%
CCTAACTGAATCCCTGTATC+TGG + tig0021743:104696-104715 None:intergenic 45.0%
CCAGATACAGGGATTCAGTT+AGG - tig0021743:104693-104712 Msa1393610:intron 45.0%
TAAAGAAAAAGGGGCAACCC+AGG + tig0021743:104728-104747 None:intergenic 45.0%
TATACCACTCACCAACTCCA+TGG - tig0021743:104752-104771 Msa1393610:intron 45.0%
CCTCAAAAACTCCATGGAGT+TGG + tig0021743:104766-104785 None:intergenic 45.0%
! CCAACTCCATGGAGTTTTTG+AGG - tig0021743:104763-104782 Msa1393610:intron 45.0%
TGTTACGTCGTAACATGTGG+TGG - tig0021743:104957-104976 Msa1393610:intron 45.0%
GTTACGTCGTAACATGTGGT+GGG - tig0021743:104958-104977 Msa1393610:intron 45.0%
TTACGTCGTAACATGTGGTG+GGG - tig0021743:104959-104978 Msa1393610:intron 45.0%
GTGGGGTCGTTTGTTTGTTT+AGG - tig0021743:104976-104995 Msa1393610:intron 45.0%
!! GTTTAGGCTGCAAGTTTTGG+TGG - tig0021743:104992-105011 Msa1393610:intron 45.0%
GCACCATACTAAAGCCGTAA+AGG + tig0021743:105103-105122 None:intergenic 45.0%
! GTATTGATGCAGCAGCCAAT+GGG + tig0021743:105174-105193 None:intergenic 45.0%
! TGTATTGATGCAGCAGCCAA+TGG + tig0021743:105175-105194 None:intergenic 45.0%
AACATGTGAGCCACTAGAAG+AGG - tig0021743:105290-105309 Msa1393610:CDS 45.0%
CCATCATAGTGGTGAAGAAC+TGG + tig0021743:105327-105346 None:intergenic 45.0%
CCAGTTCTTCACCACTATGA+TGG - tig0021743:105324-105343 Msa1393610:CDS 45.0%
GGAACTTAGGTCCATCATAG+TGG + tig0021743:105338-105357 None:intergenic 45.0%
TTCAGCTCAAGACGGAACTT+AGG + tig0021743:105351-105370 None:intergenic 45.0%
TGCTTCCAACGACACTTTCA+AGG - tig0021743:105515-105534 Msa1393610:CDS 45.0%
!! CACAACCTTGAAAGTGTCGT+TGG + tig0021743:105523-105542 None:intergenic 45.0%
GCTGCCAATTTACGGTTGAA+TGG - tig0021743:105853-105872 Msa1393610:five_prime_UTR 45.0%
!!! AATAATATACATTTTTTAAT+TGG + tig0021743:103853-103872 None:intergenic 5.0%
!! TTAAACAAAATTAAAAAAAT+GGG + tig0021743:104067-104086 None:intergenic 5.0%
!! ATTAAACAAAATTAAAAAAA+TGG + tig0021743:104068-104087 None:intergenic 5.0%
!!! TTTTATTTTTTCTATATATT+TGG - tig0021743:104303-104322 Msa1393610:intron 5.0%
!! ATGAAATAATTAAAAAAAAA+GGG + tig0021743:105424-105443 None:intergenic 5.0%
!! AATGAAATAATTAAAAAAAA+AGG + tig0021743:105425-105444 None:intergenic 5.0%
TTCCTAGGAAGAGACTCTGC+TGG + tig0021743:103482-103501 None:intergenic 50.0%
GTCTTAAATGGGCCCTCAAG+GGG + tig0021743:103952-103971 None:intergenic 50.0%
CTGTCTTAAATGGGCCCTCA+AGG + tig0021743:103954-103973 None:intergenic 50.0%
!! AGCATAGTCTTAGCTTGCGG+TGG - tig0021743:104031-104050 Msa1393610:CDS 50.0%
ATAACATATACCCCCGGCAG+CGG + tig0021743:104357-104376 None:intergenic 50.0%
TAGGTTTGCCTCCCAGATAC+AGG - tig0021743:104681-104700 Msa1393610:intron 50.0%
AGGTTTGCCTCCCAGATACA+GGG - tig0021743:104682-104701 Msa1393610:intron 50.0%
ACTGAATCCCTGTATCTGGG+AGG + tig0021743:104692-104711 None:intergenic 50.0%
TCAGTTAGGGCCATTACTCC+TGG - tig0021743:104707-104726 Msa1393610:intron 50.0%
CAGTTAGGGCCATTACTCCT+GGG - tig0021743:104708-104727 Msa1393610:intron 50.0%
!! AACTCCATGGAGTTGGTGAG+TGG + tig0021743:104759-104778 None:intergenic 50.0%
! TATTGATCCAACGGCTTGGG+AGG - tig0021743:105021-105040 Msa1393610:intron 50.0%
CAGGCACATTCAGCTCAAGA+CGG + tig0021743:105359-105378 None:intergenic 50.0%
TCTTGAGCTGAATGTGCCTG+AGG - tig0021743:105359-105378 Msa1393610:CDS 50.0%
! GTTGTAGGCATTGTCTCTGC+TGG - tig0021743:105473-105492 Msa1393610:CDS 50.0%
CTAGGAAGAGACTCTGCTGG+TGG + tig0021743:103479-103498 None:intergenic 55.0%
CACCAGCAGAGTCTCTTCCT+AGG - tig0021743:103477-103496 Msa1393610:CDS 55.0%
GGTATTCGGCCTAGATCCGA+CGG - tig0021743:103916-103935 Msa1393610:intron 55.0%
TAAATGGGCCCTCAAGGGGT+GGG + tig0021743:103948-103967 None:intergenic 55.0%
TTAAATGGGCCCTCAAGGGG+TGG + tig0021743:103949-103968 None:intergenic 55.0%
AAGGGGCAACCCAGGAGTAA+TGG + tig0021743:104720-104739 None:intergenic 55.0%
TTCTTGTCCTCCCAAGCCGT+TGG + tig0021743:105031-105050 None:intergenic 55.0%
! TGGGAGGACAAGAAGTGTGC+TGG - tig0021743:105037-105056 Msa1393610:intron 55.0%
CGAATCTCGTTTCCCTGCTC+AGG - tig0021743:105060-105079 Msa1393610:intron 55.0%
TTTAGACGGTCCCGTCCCAT+TGG - tig0021743:105156-105175 Msa1393610:intron 55.0%
TGATGCAGCAGCCAATGGGA+CGG + tig0021743:105170-105189 None:intergenic 55.0%
TCTCTTGTCAGCAGCCGCCT+TGG + tig0021743:103444-103463 None:intergenic 60.0%
GGGATTGGACCGTCGGATCT+AGG + tig0021743:103928-103947 None:intergenic 60.0%
AAGGGGTGGGATTGGACCGT+CGG + tig0021743:103935-103954 None:intergenic 60.0%
GGTCCAATCCCACCCCTTGA+GGG - tig0021743:103937-103956 Msa1393610:intron 60.0%
!! GTCTTAGCTTGCGGTGGCCT+TGG - tig0021743:104037-104056 Msa1393610:CDS 60.0%
!! TTGGCTAGCTTTGCCGCTGC+CGG - tig0021743:104341-104360 Msa1393610:intron 60.0%
GATGCAGCAGCCAATGGGAC+GGG + tig0021743:105169-105188 None:intergenic 60.0%
CGGTCCAATCCCACCCCTTG+AGG - tig0021743:103936-103955 Msa1393610:intron 65.0%
!! TGGCTAGCTTTGCCGCTGCC+GGG - tig0021743:104342-104361 Msa1393610:intron 65.0%
GGGCCCTCAAGGGGTGGGAT+TGG + tig0021743:103943-103962 None:intergenic 70.0%
!! GGCTAGCTTTGCCGCTGCCG+GGG - tig0021743:104343-104362 Msa1393610:intron 70.0%
!! GCTAGCTTTGCCGCTGCCGG+GGG - tig0021743:104344-104363 Msa1393610:intron 70.0%
Chromosome Type Strat End Strand Name
tig0021743 gene 102822 105959 102822 ID=Msa1393610;Name=Msa1393610
tig0021743 mRNA 102822 105959 102822 ID=Msa1393610-mRNA-1;Parent=Msa1393610;Name=Msa1393610-mRNA-1;_AED=0.09;_eAED=0.09;_QI=156|1|1|1|1|1|5|408|321
tig0021743 exon 102822 103301 102822 ID=Msa1393610-mRNA-1:exon:13875;Parent=Msa1393610-mRNA-1
tig0021743 exon 103401 103511 103401 ID=Msa1393610-mRNA-1:exon:13874;Parent=Msa1393610-mRNA-1
tig0021743 exon 103700 103783 103700 ID=Msa1393610-mRNA-1:exon:13873;Parent=Msa1393610-mRNA-1
tig0021743 exon 103997 104097 103997 ID=Msa1393610-mRNA-1:exon:13872;Parent=Msa1393610-mRNA-1
tig0021743 exon 105206 105959 105206 ID=Msa1393610-mRNA-1:exon:13871;Parent=Msa1393610-mRNA-1
tig0021743 five_prime_UTR 105804 105959 105804 ID=Msa1393610-mRNA-1:five_prime_utr;Parent=Msa1393610-mRNA-1
tig0021743 CDS 105206 105803 105206 ID=Msa1393610-mRNA-1:cds;Parent=Msa1393610-mRNA-1
tig0021743 CDS 103997 104097 103997 ID=Msa1393610-mRNA-1:cds;Parent=Msa1393610-mRNA-1
tig0021743 CDS 103700 103783 103700 ID=Msa1393610-mRNA-1:cds;Parent=Msa1393610-mRNA-1
tig0021743 CDS 103401 103511 103401 ID=Msa1393610-mRNA-1:cds;Parent=Msa1393610-mRNA-1
tig0021743 CDS 103230 103301 103230 ID=Msa1393610-mRNA-1:cds;Parent=Msa1393610-mRNA-1
tig0021743 three_prime_UTR 102822 103229 102822 ID=Msa1393610-mRNA-1:three_prime_utr;Parent=Msa1393610-mRNA-1
Gene Sequence

>Msa1393610

ATGGAGAACAACCATAACAATCAACAATCTTTTTGGCAATTCAGTGATCAACTTCGTGTCCACACATCAAATCTAGCAAACCTATCTCTTAATGATTCAATCTGGGGAAACAATTACTCATCCTCCAAAAATCAAAGGAGAAATTTTGATACAAAACTTGGTGGTGAAATCATCAACAACAACAACAACTCTGTTTCTCTTGGAAAATCCCCTGTTTCTGATCATTTCAATAATCAAGGATCATCTTTTTTCTCTTTTGATGTTGGTGTTTCTCCTATTAATGGAGGTTTTAATAAGGGAATTTATTCAAAGCCTTCTTATGGTGGGAATTTTAATAGTAACATGAATGTTACTAAAGGTGAAGATGAGATTTTTCATCCATCTAAATCTTCCAAGAAGAATTCAAACCCTAATAAAAAACAAGGGGAGAACAATAACAGTGATGGAAATAAGAACAAGGATTCCAAGGCGGCTGCTGACAAGAGATTCAAGACTTTGCCACCAGCAGAGTCTCTTCCTAGGAATGAAACCATTGGTGGGTATATCTTTGTCTGTAACAATGATACCATGGCTGAGAATCTCAAAAGGCAACTCTTTGGTTTGCCTCCCAGATACAGGGATTCAGTTAGGGCCATTACTCCTGGGTTGCCCCTTTTTCTTTACAACTATACCACTCACCAACTCCATGGAGTTTTTGAGGCTGCAAGTTTTGGTGGAACAAATATTGATCCAACGGCTTGGGAGGACAAGAAGTGTGCTGGCGAATCTCGTTTCCCTGCTCAGGTAAGAGTGCTTACTAGGAAAACATGTGAGCCACTAGAAGAGGATTCATTCAGGCCAGTTCTTCACCACTATGATGGACCTAAGTTCCGTCTTGAGCTGAATGTGCCTGAGGCATTGTCTCTGCTGGATATTTTTGAAGAACAAGATGCTTCCAACGACACTTTCAAGGTTGTGTCAGCATAA
Protein sequence

>Msa1393610

MENNHNNQQSFWQFSDQLRVHTSNLANLSLNDSIWGNNYSSSKNQRRNFDTKLGGEIINNNNNSVSLGKSPVSDHFNNQGSSFFSFDVGVSPINGGFNKGIYSKPSYGGNFNSNMNVTKGEDEIFHPSKSSKKNSNPNKKQGENNNSDGNKNKDSKAAADKRFKTLPPAESLPRNETIGGYIFVCNNDTMAENLKRQLFGLPPRYRDSVRAITPGLPLFLYNYTTHQLHGVFEAASFGGTNIDPTAWEDKKCAGESRFPAQVRVLTRKTCEPLEEDSFRPVLHHYDGPKFRLELNVPEALSLLDIFEEQDASNDTFKVVSA*