AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0572850

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0572850 MtrunA17_Chr8g0388501 96.584 322 10 1 1 321 1 322 0.0 636
Msa0572850 MtrunA17_Chr3g0120061 59.763 338 112 5 1 321 1 331 8.54e-130 373
Msa0572850 MtrunA17_Chr7g0225451 51.402 321 113 8 6 308 2 297 1.25e-98 293
Msa0572850 MtrunA17_Chr7g0243491 48.305 118 57 2 168 282 20 136 5.79e-26 106
Msa0572850 MtrunA17_Chr3g0081541 27.451 306 190 7 34 321 86 377 7.83e-20 90.1
Msa0572850 MtrunA17_Chr4g0043691 37.692 130 75 2 180 307 19 144 4.91e-19 88.2
Msa0572850 MtrunA17_Chr2g0311141 34.437 151 88 3 163 306 93 239 5.24e-19 87.4
Msa0572850 MtrunA17_Chr3g0131611 36.567 134 70 3 178 306 14 137 9.34e-18 83.6
Msa0572850 MtrunA17_Chr3g0081571 34.815 135 79 3 174 306 306 433 2.66e-16 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0572850 AT5G42050.1 59.155 355 93 9 1 310 1 348 1.74e-121 353
Msa0572850 AT3G27090.1 52.805 303 126 6 10 308 3 292 6.81e-98 291
Msa0572850 AT3G27090.2 52.805 303 126 6 10 308 3 292 6.81e-98 291
Msa0572850 AT3G27090.3 52.805 303 126 6 10 308 3 292 6.81e-98 291
Msa0572850 AT5G01660.3 41.667 120 67 2 178 295 18 136 5.13e-25 105
Msa0572850 AT5G01660.2 41.667 120 67 2 178 295 18 136 8.05e-25 105
Msa0572850 AT5G01660.1 41.667 120 67 2 178 295 18 136 8.05e-25 105
Msa0572850 AT3G11000.1 42.424 132 73 2 178 307 11 141 2.53e-24 103
Msa0572850 AT3G11000.2 42.424 132 73 2 178 307 130 260 7.13e-24 102
Msa0572850 AT5G61910.3 37.500 136 77 2 172 306 59 187 1.81e-22 98.6
Msa0572850 AT5G61910.1 37.500 136 77 2 172 306 55 183 1.89e-22 98.6
Msa0572850 AT5G61910.2 37.500 136 77 2 172 306 55 183 1.89e-22 98.6
Msa0572850 AT5G61910.5 37.500 136 77 2 172 306 55 183 2.42e-22 98.2
Msa0572850 AT5G61910.4 37.500 136 77 2 172 306 55 183 7.79e-21 94.0
Msa0572850 AT2G35140.6 34.722 144 88 3 180 321 49 188 1.12e-18 87.4
Msa0572850 AT2G35140.5 34.722 144 88 3 180 321 23 162 1.19e-18 87.4
Msa0572850 AT2G35140.1 34.722 144 88 3 180 321 23 162 1.19e-18 87.4
Msa0572850 AT2G35140.4 34.722 144 88 3 180 321 23 162 1.19e-18 87.4
Msa0572850 AT2G35140.2 34.722 144 88 3 180 321 23 162 1.19e-18 87.4
Msa0572850 AT2G35140.3 34.722 144 88 3 180 321 23 162 1.19e-18 87.4
Msa0572850 AT2G32910.1 29.536 237 141 9 78 306 224 442 3.10e-18 85.9

Find 85 sgRNAs with CRISPR-Local

Find 218 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGGTTGTGTCAGCATAAA+AGG 0.132335 4_1:-75823214 Msa0572850:exon
ACTTGATATGTTTCAACTTC+TGG 0.192127 4_1:-75822811 Msa0572850:three_prime_UTR
CGGTTGAATGGTTCATTATA+GGG 0.238859 4_1:-75822876 Msa0572850:three_prime_UTR
CCTAACTGAATCCCTGTATC+TGG 0.267819 4_1:+75824052 None:intergenic
TGGTATAGTTGTAAAGAAAA+AGG 0.278735 4_1:+75824009 None:intergenic
GTTGGTGTTTCTCCTATTAA+TGG 0.279410 4_1:-75825505 Msa0572850:CDS
TTATTAAAACCTCCATTAAT+AGG 0.287108 4_1:+75825493 None:intergenic
ATACTAGATAATTAGAGATT+TGG 0.288893 4_1:-75823131 Msa0572850:three_prime_UTR
ACGGTTGAATGGTTCATTAT+AGG 0.296900 4_1:-75822877 Msa0572850:three_prime_UTR
TATGCAAAGCTGCCAATTTA+CGG 0.297962 4_1:-75822896 Msa0572850:three_prime_UTR
TGAACCATTCAACCGTAAAT+TGG 0.312036 4_1:+75822884 None:intergenic
ATTTATTCAAAGCCTTCTTA+TGG 0.312416 4_1:-75825466 Msa0572850:CDS
TCAGTTAGGGCCATTACTCC+TGG 0.331278 4_1:-75824038 Msa0572850:CDS
ATTAGGGTTTGAATTCTTCT+TGG 0.332710 4_1:+75825375 None:intergenic
TATCTCTTAATGATTCAATC+TGG 0.342706 4_1:-75825684 Msa0572850:CDS
TACTAGATAATTAGAGATTT+GGG 0.346604 4_1:-75823130 Msa0572850:three_prime_UTR
AGATTGAATCATTAAGAGAT+AGG 0.365054 4_1:+75825686 None:intergenic
TAGGTTTGCCTCCCAGATAC+AGG 0.367382 4_1:-75824064 Msa0572850:CDS
CCAGATACAGGGATTCAGTT+AGG 0.380760 4_1:-75824052 Msa0572850:CDS
CCACCAATGGTTTCATTCCT+AGG 0.384980 4_1:+75825250 None:intergenic
TTCTTCTTGGAAGATTTAGA+TGG 0.386595 4_1:+75825388 None:intergenic
AACAACAACTCTGTTTCTCT+TGG 0.386776 4_1:-75825586 Msa0572850:CDS
TTATTGAAATGATCAGAAAC+AGG 0.392314 4_1:+75825556 None:intergenic
TTTGTTTAGGCTGCAAGTTT+TGG 0.392417 4_1:-75823756 Msa0572850:intron
CAAATTACTACAAAGAATAA+AGG 0.401147 4_1:-75823180 Msa0572850:three_prime_UTR
TGCTTCCAATCACACTTTCA+AGG 0.410104 4_1:-75823232 Msa0572850:CDS
TCTGATCATTTCAATAATCA+AGG 0.410681 4_1:-75825550 Msa0572850:CDS
GCAGCTCTATAACAATATAT+AGG 0.417233 4_1:+75823031 None:intergenic
AATCTCAAAAGGCAACTCTT+TGG 0.419984 4_1:-75825190 Msa0572850:intron
CCATCATAGTGGTGAAGAAC+TGG 0.427123 4_1:+75823423 None:intergenic
ATCTCTTAATGATTCAATCT+GGG 0.445296 4_1:-75825683 Msa0572850:CDS
TTAAAATTCCCACCATAAGA+AGG 0.446938 4_1:+75825454 None:intergenic
AAATATTGATCCAACGGCTT+GGG 0.453134 4_1:-75823727 Msa0572850:CDS
CACTAGAAGAGGATTCATTC+AGG 0.455058 4_1:-75823446 Msa0572850:CDS
CGAATCTCGTTTCCCTGCTC+AGG 0.457693 4_1:-75823685 Msa0572850:intron
TACAGGTAAGAGTGCTTACT+AGG 0.458108 4_1:-75823482 Msa0572850:intron
GCTGCCAATTTACGGTTGAA+TGG 0.466789 4_1:-75822888 Msa0572850:three_prime_UTR
TCTCTTGTCAGCAGCCGCCT+TGG 0.468255 4_1:+75825303 None:intergenic
CACAACCTTGAAAGTGTGAT+TGG 0.470760 4_1:+75823227 None:intergenic
TTCTTGTCCTCCCAAGCCGT+TGG 0.480092 4_1:+75823717 None:intergenic
CAGTTAGGGCCATTACTCCT+GGG 0.491247 4_1:-75824037 Msa0572850:CDS
AATGAAAATGATACCTGAGC+AGG 0.497887 4_1:+75823672 None:intergenic
AAGGTTGTGTCAGCATAAAA+GGG 0.500936 4_1:-75823213 Msa0572850:exon
GTTGTAGGCATTGTCTCTGC+TGG 0.511637 4_1:-75823274 Msa0572850:intron
CAGATACAGGGATTCAGTTA+GGG 0.515717 4_1:-75824051 Msa0572850:CDS
CCAGTTCTTCACCACTATGA+TGG 0.516230 4_1:-75823423 Msa0572850:CDS
TGGGAGGACAAGAAGTGTGC+TGG 0.518213 4_1:-75823708 Msa0572850:CDS
TATTCAAAGCCTTCTTATGG+TGG 0.524188 4_1:-75825463 Msa0572850:CDS
CAAATATTGATCCAACGGCT+TGG 0.524783 4_1:-75823728 Msa0572850:CDS
CTAACTGAATCCCTGTATCT+GGG 0.538745 4_1:+75824053 None:intergenic
AAGGGGCAACCCAGGAGTAA+TGG 0.541588 4_1:+75824028 None:intergenic
ATTCAAAGCCTTCTTATGGT+GGG 0.541890 4_1:-75825462 Msa0572850:CDS
TATTGATCCAACGGCTTGGG+AGG 0.545418 4_1:-75823724 Msa0572850:CDS
GGTGTTTCTCCTATTAATGG+AGG 0.546103 4_1:-75825502 Msa0572850:CDS
AGTAACATGAATGTTACTAA+AGG 0.551944 4_1:-75825430 Msa0572850:CDS
CTGAATGAATCCTCTTCTAG+TGG 0.554234 4_1:+75823447 None:intergenic
CCATGGCTGAGAATCTCAAA+AGG 0.555325 4_1:-75825201 Msa0572850:CDS
AGGTTTGCCTCCCAGATACA+GGG 0.571707 4_1:-75824063 Msa0572850:CDS
CACCAGCAGAGTCTCTTCCT+AGG 0.573046 4_1:-75825267 Msa0572850:CDS
GGGGAGAACAATAACAGTGA+TGG 0.573073 4_1:-75825343 Msa0572850:CDS
CCTAGGAATGAAACCATTGG+TGG 0.576885 4_1:-75825250 Msa0572850:CDS
TTCAGCTCAAGACGGAACTT+AGG 0.577473 4_1:+75823399 None:intergenic
GGAACTTAGGTCCATCATAG+TGG 0.580135 4_1:+75823412 None:intergenic
TATACCACTCACCAACTCCA+TGG 0.580365 4_1:-75823993 Msa0572850:CDS
CTTCCTAGGAATGAAACCAT+TGG 0.584075 4_1:-75825253 Msa0572850:CDS
TTCCTAGGAAGAGACTCTGC+TGG 0.585025 4_1:+75825265 None:intergenic
AACATGTGAGCCACTAGAAG+AGG 0.589083 4_1:-75823457 Msa0572850:CDS
ATGAAAATGATACCTGAGCA+GGG 0.598544 4_1:+75823673 None:intergenic
GGTTTGCTAGATTTGATGTG+TGG 0.599698 4_1:+75825707 None:intergenic
TCTCTTAATGATTCAATCTG+GGG 0.606044 4_1:-75825682 Msa0572850:CDS
TGGAACAAATATTGATCCAA+CGG 0.610123 4_1:-75823733 Msa0572850:CDS
AAATAAGAACAAGGATTCCA+AGG 0.613003 4_1:-75825320 Msa0572850:CDS
CAGTGATGGAAATAAGAACA+AGG 0.622810 4_1:-75825329 Msa0572850:CDS
AACTCCATGGAGTTGGTGAG+TGG 0.632771 4_1:+75823989 None:intergenic
TATTGAAATGATCAGAAACA+GGG 0.638940 4_1:+75825557 None:intergenic
CTAGGAATGAAACCATTGGT+GGG 0.642582 4_1:-75825249 Msa0572850:CDS
GACAAAGATATACCCACCAA+TGG 0.644157 4_1:+75825237 None:intergenic
CTAGGAAGAGACTCTGCTGG+TGG 0.665578 4_1:+75825268 None:intergenic
CAGGCACATTCAGCTCAAGA+CGG 0.668323 4_1:+75823391 None:intergenic
TCTTGAGCTGAATGTGCCTG+AGG 0.674355 4_1:-75823388 Msa0572850:intron
TTACATATACACATACACAT+AGG 0.682964 4_1:-75825817 Msa0572850:five_prime_UTR
TGTCTGTAACAATGATACCA+TGG 0.683554 4_1:-75825218 Msa0572850:CDS
ATTGAAATGATCAGAAACAG+GGG 0.684933 4_1:+75825558 None:intergenic
ACTGAATCCCTGTATCTGGG+AGG 0.733702 4_1:+75824056 None:intergenic
TAAGAACAAGGATTCCAAGG+CGG 0.748891 4_1:-75825317 Msa0572850:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTGACAAAATTTTTTTA+AGG - chr4_1:75823702-75823721 Msa0572850:CDS 10.0%
!!! AAAATTTTTTTAAGGTAAAA+AGG - chr4_1:75823710-75823729 Msa0572850:CDS 10.0%
!! TAAACAAAATTAAAAAAATG+GGG + chr4_1:75824044-75824063 None:intergenic 10.0%
!! ATAATATATTTACTATAGAT+TGG - chr4_1:75824493-75824512 Msa0572850:intron 10.0%
!! TGAAATAATTAAAAAAAAAG+GGG + chr4_1:75825400-75825419 None:intergenic 10.0%
!! TTATTTGAAAATTCAGTATT+CGG - chr4_1:75823881-75823900 Msa0572850:intron 15.0%
!! AAGTAAAATTATATATGAAC+TGG - chr4_1:75823952-75823971 Msa0572850:intron 15.0%
!!! TATAAGTTAAAGTGTAATTT+TGG - chr4_1:75824072-75824091 Msa0572850:intron 15.0%
!!! ATAAGTTAAAGTGTAATTTT+GGG - chr4_1:75824073-75824092 Msa0572850:intron 15.0%
!! GAAATAATTAAAAAAAAAGG+GGG + chr4_1:75825399-75825418 None:intergenic 15.0%
!!! TTTGTTTTTATATATAGCTA+CGG - chr4_1:75825617-75825636 Msa0572850:CDS 15.0%
!!! AATTGTTTTTTACATATCTA+CGG + chr4_1:75825658-75825677 None:intergenic 15.0%
!!! TTTCAAGTTTTGTGAAAAAA+TGG - chr4_1:75822939-75822958 Msa0572850:three_prime_UTR 20.0%
!!! AAAAAGATTGTTGATTGTTA+TGG + chr4_1:75822972-75822991 None:intergenic 20.0%
!!! ATAACAATCAACAATCTTTT+TGG - chr4_1:75822971-75822990 Msa0572850:three_prime_UTR 20.0%
!!! AAAATTTCTCCTTTGATTTT+TGG + chr4_1:75823085-75823104 None:intergenic 20.0%
!!! AGAAATTTTGATACAAAACT+TGG - chr4_1:75823096-75823115 Msa0572850:three_prime_UTR 20.0%
!! TTATTAAAACCTCCATTAAT+AGG + chr4_1:75823234-75823253 None:intergenic 20.0%
!!! TATTAATGGAGGTTTTAATA+AGG - chr4_1:75823233-75823252 Msa0572850:CDS 20.0%
!!! ATTAATGGAGGTTTTAATAA+GGG - chr4_1:75823234-75823253 Msa0572850:CDS 20.0%
!! ATAATTGAAGGAAACAATTT+AGG + chr4_1:75823576-75823595 None:intergenic 20.0%
!! AATAAGAATTGCATAATTGA+AGG + chr4_1:75823588-75823607 None:intergenic 20.0%
!! TAATCGAACACAAAAAAAAT+CGG + chr4_1:75823658-75823677 None:intergenic 20.0%
!!! ATTTTACTTCTGTCTTAAAT+GGG + chr4_1:75823941-75823960 None:intergenic 20.0%
!!! AATTTTACTTCTGTCTTAAA+TGG + chr4_1:75823942-75823961 None:intergenic 20.0%
!!! ATTTTCGAAGTAATTTATTC+CGG + chr4_1:75824131-75824150 None:intergenic 20.0%
!! GTAAATATATTATGCTTCTA+TGG + chr4_1:75824486-75824505 None:intergenic 20.0%
!! AAAAACATAATTGATACTGA+AGG + chr4_1:75824614-75824633 None:intergenic 20.0%
!!! CACAATTTTAATGGATAATT+CGG + chr4_1:75824838-75824857 None:intergenic 20.0%
!! CAAAATGTATTCAAATTATG+AGG + chr4_1:75824891-75824910 None:intergenic 20.0%
!!! CTCATAATTTGAATACATTT+TGG - chr4_1:75824889-75824908 Msa0572850:intron 20.0%
!! TCTTTAACATTGATAAATAC+AGG - chr4_1:75825225-75825244 Msa0572850:CDS 20.0%
!! CAAATTACTACAAAGAATAA+AGG - chr4_1:75825544-75825563 Msa0572850:CDS 20.0%
!! ATACTAGATAATTAGAGATT+TGG - chr4_1:75825593-75825612 Msa0572850:CDS 20.0%
!! TACTAGATAATTAGAGATTT+GGG - chr4_1:75825594-75825613 Msa0572850:CDS 20.0%
!! TATGTAAAAAACAATTTGAG+AGG - chr4_1:75825662-75825681 Msa0572850:CDS 20.0%
! TTACATATACACATACACAT+AGG - chr4_1:75822907-75822926 Msa0572850:three_prime_UTR 25.0%
! AGATTGAATCATTAAGAGAT+AGG + chr4_1:75823041-75823060 None:intergenic 25.0%
! TATCTCTTAATGATTCAATC+TGG - chr4_1:75823040-75823059 Msa0572850:three_prime_UTR 25.0%
! ATCTCTTAATGATTCAATCT+GGG - chr4_1:75823041-75823060 Msa0572850:three_prime_UTR 25.0%
!!! AATTTTGATACAAAACTTGG+TGG - chr4_1:75823099-75823118 Msa0572850:three_prime_UTR 25.0%
! TATTGAAATGATCAGAAACA+GGG + chr4_1:75823170-75823189 None:intergenic 25.0%
! TTATTGAAATGATCAGAAAC+AGG + chr4_1:75823171-75823190 None:intergenic 25.0%
! TCTGATCATTTCAATAATCA+AGG - chr4_1:75823174-75823193 Msa0572850:three_prime_UTR 25.0%
!!! TCTTTTTTCTCTTTTGATGT+TGG - chr4_1:75823201-75823220 Msa0572850:exon 25.0%
! ATTTATTCAAAGCCTTCTTA+TGG - chr4_1:75823258-75823277 Msa0572850:CDS 25.0%
! AGTAACATGAATGTTACTAA+AGG - chr4_1:75823294-75823313 Msa0572850:intron 25.0%
! TCAAACCCTAATAAAAAACA+AGG - chr4_1:75823360-75823379 Msa0572850:intron 25.0%
! CAAACCCTAATAAAAAACAA+GGG - chr4_1:75823361-75823380 Msa0572850:intron 25.0%
! AAACCCTAATAAAAAACAAG+GGG - chr4_1:75823362-75823381 Msa0572850:intron 25.0%
! ATAATATGGATTTAACTACC+CGG - chr4_1:75824109-75824128 Msa0572850:intron 25.0%
!! TTTTCGAAGTAATTTATTCC+GGG + chr4_1:75824130-75824149 None:intergenic 25.0%
! TTCTCTATGTACTTACTATA+GGG + chr4_1:75824230-75824249 None:intergenic 25.0%
!!! TTTATTTTGGTTACCATGTA+TGG - chr4_1:75824374-75824393 Msa0572850:intron 25.0%
!!! TTATTTTGGTTACCATGTAT+GGG - chr4_1:75824375-75824394 Msa0572850:intron 25.0%
! AACAATAACTGCAAAATTTC+AGG + chr4_1:75824457-75824476 None:intergenic 25.0%
! AATGTCAATTGCAATATTTG+AGG - chr4_1:75824557-75824576 Msa0572850:intron 25.0%
!! TTGTGAATGTTTGTGTTTTT+AGG - chr4_1:75824641-75824660 Msa0572850:intron 25.0%
! GTATAGTTGTAAAGAAAAAG+GGG + chr4_1:75824716-75824735 None:intergenic 25.0%
! GGTATAGTTGTAAAGAAAAA+GGG + chr4_1:75824717-75824736 None:intergenic 25.0%
! TGGTATAGTTGTAAAGAAAA+AGG + chr4_1:75824718-75824737 None:intergenic 25.0%
! ATCCATTAAAATTGTGCATT+AGG - chr4_1:75824842-75824861 Msa0572850:intron 25.0%
!! TAAACTGTGCTTTCTTTTAT+GGG - chr4_1:75825428-75825447 Msa0572850:CDS 25.0%
!! TGTCCAATTTTTCTTTTGTT+TGG - chr4_1:75825726-75825745 Msa0572850:CDS 25.0%
!!! GTCCAATTTTTCTTTTGTTT+GGG - chr4_1:75825727-75825746 Msa0572850:CDS 25.0%
! ATCCCAAACAAAAGAAAAAT+TGG + chr4_1:75825732-75825751 None:intergenic 25.0%
!! GTATTGAACTATTTTGCTTT+TGG - chr4_1:75825801-75825820 Msa0572850:five_prime_UTR 25.0%
TCTCTTAATGATTCAATCTG+GGG - chr4_1:75823042-75823061 Msa0572850:three_prime_UTR 30.0%
!! ATTTCTCCTTTGATTTTTGG+AGG + chr4_1:75823082-75823101 None:intergenic 30.0%
ATTGAAATGATCAGAAACAG+GGG + chr4_1:75823169-75823188 None:intergenic 30.0%
TTAAAATTCCCACCATAAGA+AGG + chr4_1:75823273-75823292 None:intergenic 30.0%
TTCTTCTTGGAAGATTTAGA+TGG + chr4_1:75823339-75823358 None:intergenic 30.0%
ATTAGGGTTTGAATTCTTCT+TGG + chr4_1:75823352-75823371 None:intergenic 30.0%
!!! TCTCCCCTTGTTTTTTATTA+GGG + chr4_1:75823368-75823387 None:intergenic 30.0%
!!! TTCTCCCCTTGTTTTTTATT+AGG + chr4_1:75823369-75823388 None:intergenic 30.0%
AAATAAGAACAAGGATTCCA+AGG - chr4_1:75823404-75823423 Msa0572850:CDS 30.0%
GGAAACAATTTAGGAAAGAA+AGG + chr4_1:75823567-75823586 None:intergenic 30.0%
GTTTAGCTACTCGAATAATA+TGG - chr4_1:75824095-75824114 Msa0572850:intron 30.0%
GTTCTCTATGTACTTACTAT+AGG + chr4_1:75824231-75824250 None:intergenic 30.0%
!!! GTCTATCTGTAGCTTTATTT+TGG - chr4_1:75824361-75824380 Msa0572850:intron 30.0%
!!! TATTTTGGTTACCATGTATG+GGG - chr4_1:75824376-75824395 Msa0572850:intron 30.0%
TGATATCAATATCACTGTAC+TGG - chr4_1:75824399-75824418 Msa0572850:intron 30.0%
ACAGGTTATTATCCCTTATA+TGG + chr4_1:75824527-75824546 None:intergenic 30.0%
TTGACATTGAATTGAAGAAC+AGG + chr4_1:75824545-75824564 None:intergenic 30.0%
!!! TTTTTGAGGTATGTGTTACT+TGG - chr4_1:75824756-75824775 Msa0572850:intron 30.0%
ATCTATGAGACTGTTAGTTA+GGG - chr4_1:75824809-75824828 Msa0572850:intron 30.0%
! TGCCTAATGCACAATTTTAA+TGG + chr4_1:75824847-75824866 None:intergenic 30.0%
ATGTATTCAAATTATGAGGC+TGG + chr4_1:75824887-75824906 None:intergenic 30.0%
TGGAACAAATATTGATCCAA+CGG - chr4_1:75824991-75825010 Msa0572850:intron 30.0%
!!! TGTTTTGATCTTCTAGTTTG+TGG - chr4_1:75825186-75825205 Msa0572850:intron 30.0%
! CTAAACTGTGCTTTCTTTTA+TGG - chr4_1:75825427-75825446 Msa0572850:CDS 30.0%
GCAGCTCTATAACAATATAT+AGG + chr4_1:75825696-75825715 None:intergenic 30.0%
ACTTGATATGTTTCAACTTC+TGG - chr4_1:75825913-75825932 Msa0572850:five_prime_UTR 30.0%
AACAGAGAACAAAAAGCAGT+TGG + chr4_1:75822870-75822889 None:intergenic 35.0%
ACTCATCCTCCAAAAATCAA+AGG - chr4_1:75823073-75823092 Msa0572850:three_prime_UTR 35.0%
! AACAACAACTCTGTTTCTCT+TGG - chr4_1:75823138-75823157 Msa0572850:three_prime_UTR 35.0%
! GTTGGTGTTTCTCCTATTAA+TGG - chr4_1:75823219-75823238 Msa0572850:CDS 35.0%
TATTCAAAGCCTTCTTATGG+TGG - chr4_1:75823261-75823280 Msa0572850:CDS 35.0%
ATTCAAAGCCTTCTTATGGT+GGG - chr4_1:75823262-75823281 Msa0572850:CDS 35.0%
! CAGTGATGGAAATAAGAACA+AGG - chr4_1:75823395-75823414 Msa0572850:CDS 35.0%
TGTCTGTAACAATGATACCA+TGG - chr4_1:75823506-75823525 Msa0572850:intron 35.0%
AATCTCAAAAGGCAACTCTT+TGG - chr4_1:75823534-75823553 Msa0572850:intron 35.0%
!! AGCTAGCCAAATTTTGATGA+GGG + chr4_1:75824309-75824328 None:intergenic 35.0%
!! AAGCTAGCCAAATTTTGATG+AGG + chr4_1:75824310-75824329 None:intergenic 35.0%
AATCAGATAACATATACCCC+CGG + chr4_1:75824341-75824360 None:intergenic 35.0%
TATTGATATCACCCCATACA+TGG + chr4_1:75824390-75824409 None:intergenic 35.0%
ATTGGTGTGTTAGCCATATA+AGG - chr4_1:75824511-75824530 Msa0572850:intron 35.0%
TTGGTGTGTTAGCCATATAA+GGG - chr4_1:75824512-75824531 Msa0572850:intron 35.0%
ACAGTCTCATAGATGTGATT+AGG + chr4_1:75824803-75824822 None:intergenic 35.0%
CATCTATGAGACTGTTAGTT+AGG - chr4_1:75824808-75824827 Msa0572850:intron 35.0%
TAATGTTACGTCGTAACATG+TGG - chr4_1:75824933-75824952 Msa0572850:intron 35.0%
! TTTGTTTAGGCTGCAAGTTT+TGG - chr4_1:75824968-75824987 Msa0572850:intron 35.0%
AAATATTGATCCAACGGCTT+GGG - chr4_1:75824997-75825016 Msa0572850:intron 35.0%
ATGAAAATGATACCTGAGCA+GGG + chr4_1:75825054-75825073 None:intergenic 35.0%
AATGAAAATGATACCTGAGC+AGG + chr4_1:75825055-75825074 None:intergenic 35.0%
! CATTTTCATTCCAACCTGTA+CGG - chr4_1:75825065-75825084 Msa0572850:intron 35.0%
GATGAGACAGTAGATTTAGA+CGG - chr4_1:75825119-75825138 Msa0572850:intron 35.0%
!! TGCTTTCTTTTATGGGTTGT+AGG - chr4_1:75825435-75825454 Msa0572850:CDS 35.0%
AAGGTTGTGTCAGCATAAAA+GGG - chr4_1:75825511-75825530 Msa0572850:CDS 35.0%
TATGCAAAGCTGCCAATTTA+CGG - chr4_1:75825828-75825847 Msa0572850:five_prime_UTR 35.0%
TGAACCATTCAACCGTAAAT+TGG + chr4_1:75825843-75825862 None:intergenic 35.0%
ACGGTTGAATGGTTCATTAT+AGG - chr4_1:75825847-75825866 Msa0572850:five_prime_UTR 35.0%
CGGTTGAATGGTTCATTATA+GGG - chr4_1:75825848-75825867 Msa0572850:five_prime_UTR 35.0%
!! GGTTTGCTAGATTTGATGTG+TGG + chr4_1:75823020-75823039 None:intergenic 40.0%
GGTGTTTCTCCTATTAATGG+AGG - chr4_1:75823222-75823241 Msa0572850:CDS 40.0%
TAAGAACAAGGATTCCAAGG+CGG - chr4_1:75823407-75823426 Msa0572850:CDS 40.0%
CTTCCTAGGAATGAAACCAT+TGG - chr4_1:75823471-75823490 Msa0572850:CDS 40.0%
CTAGGAATGAAACCATTGGT+GGG - chr4_1:75823475-75823494 Msa0572850:CDS 40.0%
GACAAAGATATACCCACCAA+TGG + chr4_1:75823490-75823509 None:intergenic 40.0%
AATTGACACGAGTGTATGAG+TGG - chr4_1:75823983-75824002 Msa0572850:CDS 40.0%
! TGAAGCATAGTCTTAGCTTG+CGG - chr4_1:75824006-75824025 Msa0572850:CDS 40.0%
TTAAAAAAATGGGGCAGCCA+AGG + chr4_1:75824035-75824054 None:intergenic 40.0%
!! CTAGCCAAATTTTGATGAGG+GGG + chr4_1:75824307-75824326 None:intergenic 40.0%
!! GCTAGCCAAATTTTGATGAG+GGG + chr4_1:75824308-75824327 None:intergenic 40.0%
CTAACTGAATCCCTGTATCT+GGG + chr4_1:75824674-75824693 None:intergenic 40.0%
CAGATACAGGGATTCAGTTA+GGG - chr4_1:75824673-75824692 Msa0572850:intron 40.0%
CACATACCTCAAAAACTCCA+TGG + chr4_1:75824751-75824770 None:intergenic 40.0%
CAAATATTGATCCAACGGCT+TGG - chr4_1:75824996-75825015 Msa0572850:intron 40.0%
ACTGTCTCATCTCTACTCTA+CGG + chr4_1:75825110-75825129 None:intergenic 40.0%
!! TACAGGTAAGAGTGCTTACT+AGG - chr4_1:75825242-75825261 Msa0572850:CDS 40.0%
CTGAATGAATCCTCTTCTAG+TGG + chr4_1:75825280-75825299 None:intergenic 40.0%
CACTAGAAGAGGATTCATTC+AGG - chr4_1:75825278-75825297 Msa0572850:CDS 40.0%
AGTGAACGATCAAATACCTC+AGG + chr4_1:75825355-75825374 None:intergenic 40.0%
CACTTGCGCGTATTGAATTA+TGG - chr4_1:75825371-75825390 Msa0572850:CDS 40.0%
TGCTTCCAATCACACTTTCA+AGG - chr4_1:75825492-75825511 Msa0572850:CDS 40.0%
! CACAACCTTGAAAGTGTGAT+TGG + chr4_1:75825500-75825519 None:intergenic 40.0%
CAAGGTTGTGTCAGCATAAA+AGG - chr4_1:75825510-75825529 Msa0572850:CDS 40.0%
! CAAAAAGCAGTTGGTGTGTG+TGG + chr4_1:75822861-75822880 None:intergenic 45.0%
GGGGAGAACAATAACAGTGA+TGG - chr4_1:75823381-75823400 Msa0572850:intron 45.0%
CCACCAATGGTTTCATTCCT+AGG + chr4_1:75823477-75823496 None:intergenic 45.0%
CCTAGGAATGAAACCATTGG+TGG - chr4_1:75823474-75823493 Msa0572850:CDS 45.0%
!!! CCTTTTGAGATTCTCAGCCA+TGG + chr4_1:75823526-75823545 None:intergenic 45.0%
CCATGGCTGAGAATCTCAAA+AGG - chr4_1:75823523-75823542 Msa0572850:intron 45.0%
TTGGCCCCCTCATCAAAATT+TGG - chr4_1:75824300-75824319 Msa0572850:intron 45.0%
CCTAACTGAATCCCTGTATC+TGG + chr4_1:75824675-75824694 None:intergenic 45.0%
CCAGATACAGGGATTCAGTT+AGG - chr4_1:75824672-75824691 Msa0572850:intron 45.0%
TAAAGAAAAAGGGGCAACCC+AGG + chr4_1:75824707-75824726 None:intergenic 45.0%
TATACCACTCACCAACTCCA+TGG - chr4_1:75824731-75824750 Msa0572850:intron 45.0%
CCTCAAAAACTCCATGGAGT+TGG + chr4_1:75824745-75824764 None:intergenic 45.0%
! CCAACTCCATGGAGTTTTTG+AGG - chr4_1:75824742-75824761 Msa0572850:intron 45.0%
TGTTACGTCGTAACATGTGG+TGG - chr4_1:75824936-75824955 Msa0572850:intron 45.0%
GTTACGTCGTAACATGTGGT+GGG - chr4_1:75824937-75824956 Msa0572850:intron 45.0%
TTACGTCGTAACATGTGGTG+GGG - chr4_1:75824938-75824957 Msa0572850:intron 45.0%
GTGGGGTCGTTTGTTTGTTT+AGG - chr4_1:75824955-75824974 Msa0572850:intron 45.0%
!! GTTTAGGCTGCAAGTTTTGG+TGG - chr4_1:75824971-75824990 Msa0572850:intron 45.0%
CATACTAAAGCCGTACAGGT+TGG + chr4_1:75825078-75825097 None:intergenic 45.0%
! CAACCTGTACGGCTTTAGTA+TGG - chr4_1:75825076-75825095 Msa0572850:intron 45.0%
! GTATTGATGCAGCAGCCAAT+GGG + chr4_1:75825151-75825170 None:intergenic 45.0%
! TGTATTGATGCAGCAGCCAA+TGG + chr4_1:75825152-75825171 None:intergenic 45.0%
AACATGTGAGCCACTAGAAG+AGG - chr4_1:75825267-75825286 Msa0572850:CDS 45.0%
CCATCATAGTGGTGAAGAAC+TGG + chr4_1:75825304-75825323 None:intergenic 45.0%
CCAGTTCTTCACCACTATGA+TGG - chr4_1:75825301-75825320 Msa0572850:CDS 45.0%
GGAACTTAGGTCCATCATAG+TGG + chr4_1:75825315-75825334 None:intergenic 45.0%
TTCAGCTCAAGACGGAACTT+AGG + chr4_1:75825328-75825347 None:intergenic 45.0%
GCTGCCAATTTACGGTTGAA+TGG - chr4_1:75825836-75825855 Msa0572850:five_prime_UTR 45.0%
!!! AATAATATACATTTTTTAAT+TGG + chr4_1:75823832-75823851 None:intergenic 5.0%
!! TTAAACAAAATTAAAAAAAT+GGG + chr4_1:75824045-75824064 None:intergenic 5.0%
!! ATTAAACAAAATTAAAAAAA+TGG + chr4_1:75824046-75824065 None:intergenic 5.0%
!!! TTTTATTTTTTCTATATATT+TGG - chr4_1:75824281-75824300 Msa0572850:intron 5.0%
!! ATGAAATAATTAAAAAAAAA+GGG + chr4_1:75825401-75825420 None:intergenic 5.0%
!! AATGAAATAATTAAAAAAAA+AGG + chr4_1:75825402-75825421 None:intergenic 5.0%
TTCCTAGGAAGAGACTCTGC+TGG + chr4_1:75823462-75823481 None:intergenic 50.0%
AGTATTCGGCCTAGATCCGA+CGG - chr4_1:75823895-75823914 Msa0572850:intron 50.0%
TTAAATGGGCCCTCAAGCGT+GGG + chr4_1:75823927-75823946 None:intergenic 50.0%
!! AGCATAGTCTTAGCTTGCGG+TGG - chr4_1:75824009-75824028 Msa0572850:CDS 50.0%
ATAACATATACCCCCGGCAG+CGG + chr4_1:75824335-75824354 None:intergenic 50.0%
TAGGTTTGCCTCCCAGATAC+AGG - chr4_1:75824660-75824679 Msa0572850:intron 50.0%
AGGTTTGCCTCCCAGATACA+GGG - chr4_1:75824661-75824680 Msa0572850:intron 50.0%
ACTGAATCCCTGTATCTGGG+AGG + chr4_1:75824671-75824690 None:intergenic 50.0%
TCAGTTAGGGCCATTACTCC+TGG - chr4_1:75824686-75824705 Msa0572850:intron 50.0%
CAGTTAGGGCCATTACTCCT+GGG - chr4_1:75824687-75824706 Msa0572850:intron 50.0%
!! AACTCCATGGAGTTGGTGAG+TGG + chr4_1:75824738-75824757 None:intergenic 50.0%
! TATTGATCCAACGGCTTGGG+AGG - chr4_1:75825000-75825019 Msa0572850:intron 50.0%
GCACCATACTAAAGCCGTAC+AGG + chr4_1:75825082-75825101 None:intergenic 50.0%
CAGGCACATTCAGCTCAAGA+CGG + chr4_1:75825336-75825355 None:intergenic 50.0%
TCTTGAGCTGAATGTGCCTG+AGG - chr4_1:75825336-75825355 Msa0572850:CDS 50.0%
! GTTGTAGGCATTGTCTCTGC+TGG - chr4_1:75825450-75825469 Msa0572850:CDS 50.0%
CTAGGAAGAGACTCTGCTGG+TGG + chr4_1:75823459-75823478 None:intergenic 55.0%
CACCAGCAGAGTCTCTTCCT+AGG - chr4_1:75823457-75823476 Msa0572850:CDS 55.0%
CTTAAATGGGCCCTCAAGCG+TGG + chr4_1:75823928-75823947 None:intergenic 55.0%
AAGGGGCAACCCAGGAGTAA+TGG + chr4_1:75824699-75824718 None:intergenic 55.0%
TTCTTGTCCTCCCAAGCCGT+TGG + chr4_1:75825010-75825029 None:intergenic 55.0%
! TGGGAGGACAAGAAGTGTGC+TGG - chr4_1:75825016-75825035 Msa0572850:intron 55.0%
CGAATCTCGTTTCCCTGCTC+AGG - chr4_1:75825039-75825058 Msa0572850:intron 55.0%
TTTAGACGGTCCCGTCCCAT+TGG - chr4_1:75825133-75825152 Msa0572850:intron 55.0%
TGATGCAGCAGCCAATGGGA+CGG + chr4_1:75825147-75825166 None:intergenic 55.0%
TCTCTTGTCAGCAGCCGCCT+TGG + chr4_1:75823424-75823443 None:intergenic 60.0%
GGGATTGGACCGTCGGATCT+AGG + chr4_1:75823907-75823926 None:intergenic 60.0%
CAAGCGTGGGATTGGACCGT+CGG + chr4_1:75823914-75823933 None:intergenic 60.0%
ACGGTCCAATCCCACGCTTG+AGG - chr4_1:75823914-75823933 Msa0572850:intron 60.0%
CGGTCCAATCCCACGCTTGA+GGG - chr4_1:75823915-75823934 Msa0572850:intron 60.0%
!! GTCTTAGCTTGCGGTGGCCT+TGG - chr4_1:75824015-75824034 Msa0572850:CDS 60.0%
!! TTGGCTAGCTTTGCCGCTGC+CGG - chr4_1:75824319-75824338 Msa0572850:intron 60.0%
GATGCAGCAGCCAATGGGAC+GGG + chr4_1:75825146-75825165 None:intergenic 60.0%
TGGGCCCTCAAGCGTGGGAT+TGG + chr4_1:75823922-75823941 None:intergenic 65.0%
!! TGGCTAGCTTTGCCGCTGCC+GGG - chr4_1:75824320-75824339 Msa0572850:intron 65.0%
!! GGCTAGCTTTGCCGCTGCCG+GGG - chr4_1:75824321-75824340 Msa0572850:intron 70.0%
!! GCTAGCTTTGCCGCTGCCGG+GGG - chr4_1:75824322-75824341 Msa0572850:intron 70.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 75822804 75825942 75822804 ID=Msa0572850;Name=Msa0572850
chr4_1 mRNA 75822804 75825942 75822804 ID=Msa0572850-mRNA-1;Parent=Msa0572850;Name=Msa0572850-mRNA-1;_AED=0.09;_eAED=0.09;_QI=154|1|1|1|1|1|5|414|321
chr4_1 exon 75822804 75823289 75822804 ID=Msa0572850-mRNA-1:exon:26349;Parent=Msa0572850-mRNA-1
chr4_1 exon 75823389 75823499 75823389 ID=Msa0572850-mRNA-1:exon:26348;Parent=Msa0572850-mRNA-1
chr4_1 exon 75823686 75823769 75823686 ID=Msa0572850-mRNA-1:exon:26347;Parent=Msa0572850-mRNA-1
chr4_1 exon 75823983 75824083 75823983 ID=Msa0572850-mRNA-1:exon:26346;Parent=Msa0572850-mRNA-1
chr4_1 exon 75825191 75825942 75825191 ID=Msa0572850-mRNA-1:exon:26345;Parent=Msa0572850-mRNA-1
chr4_1 five_prime_UTR 75825789 75825942 75825789 ID=Msa0572850-mRNA-1:five_prime_utr;Parent=Msa0572850-mRNA-1
chr4_1 CDS 75825191 75825788 75825191 ID=Msa0572850-mRNA-1:cds;Parent=Msa0572850-mRNA-1
chr4_1 CDS 75823983 75824083 75823983 ID=Msa0572850-mRNA-1:cds;Parent=Msa0572850-mRNA-1
chr4_1 CDS 75823686 75823769 75823686 ID=Msa0572850-mRNA-1:cds;Parent=Msa0572850-mRNA-1
chr4_1 CDS 75823389 75823499 75823389 ID=Msa0572850-mRNA-1:cds;Parent=Msa0572850-mRNA-1
chr4_1 CDS 75823218 75823289 75823218 ID=Msa0572850-mRNA-1:cds;Parent=Msa0572850-mRNA-1
chr4_1 three_prime_UTR 75822804 75823217 75822804 ID=Msa0572850-mRNA-1:three_prime_utr;Parent=Msa0572850-mRNA-1
Gene Sequence

>Msa0572850

ATGGAGAACAACCATAACAATCAACAATCTTTTTGGCAATTCAGTGATCAACTTCGTGTCCACACATCAAATCTAGCAAACCTATCTCTTAATGATTCAATCTGGGGAAACAATTACTCATCCTCCAAAAATCAAAGGAGAAATTTTGATACAAAACTTGGTGGTGAAATCATCAACAACAACAACAACTCTGTTTCTCTTGGAAAATCCCCTGTTTCTGATCATTTCAATAATCAAGGATCATCTTTTTTCTCTTTTGATGTTGGTGTTTCTCCTATTAATGGAGGTTTTAATAAGGGAATTTATTCAAAGCCTTCTTATGGTGGGAATTTTAATAGTAACATGAATGTTACTAAAGGTGAAGATGAGATTTTTCATCCATCTAAATCTTCCAAGAAGAATTCAAACCCTAATAAAAAACAAGGGGAGAACAATAACAGTGATGGAAATAAGAACAAGGATTCCAAGGCGGCTGCTGACAAGAGATTCAAGACTTTGCCACCAGCAGAGTCTCTTCCTAGGAATGAAACCATTGGTGGGTATATCTTTGTCTGTAACAATGATACCATGGCTGAGAATCTCAAAAGGCAACTCTTTGGTTTGCCTCCCAGATACAGGGATTCAGTTAGGGCCATTACTCCTGGGTTGCCCCTTTTTCTTTACAACTATACCACTCACCAACTCCATGGAGTTTTTGAGGCTGCAAGTTTTGGTGGAACAAATATTGATCCAACGGCTTGGGAGGACAAGAAGTGTGCTGGCGAATCTCGTTTCCCTGCTCAGGTAAGAGTGCTTACTAGGAAAACATGTGAGCCACTAGAAGAGGATTCATTCAGGCCAGTTCTTCACCACTATGATGGACCTAAGTTCCGTCTTGAGCTGAATGTGCCTGAGGCATTGTCTCTGCTGGATATTTTTGAAGAACAAGATGCTTCCAATCACACTTTCAAGGTTGTGTCAGCATAA
Protein sequence

>Msa0572850

MENNHNNQQSFWQFSDQLRVHTSNLANLSLNDSIWGNNYSSSKNQRRNFDTKLGGEIINNNNNSVSLGKSPVSDHFNNQGSSFFSFDVGVSPINGGFNKGIYSKPSYGGNFNSNMNVTKGEDEIFHPSKSSKKNSNPNKKQGENNNSDGNKNKDSKAAADKRFKTLPPAESLPRNETIGGYIFVCNNDTMAENLKRQLFGLPPRYRDSVRAITPGLPLFLYNYTTHQLHGVFEAASFGGTNIDPTAWEDKKCAGESRFPAQVRVLTRKTCEPLEEDSFRPVLHHYDGPKFRLELNVPEALSLLDIFEEQDASNHTFKVVSA*