AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1395330

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1395330 MtrunA17_Chr2g0290801 96.800 250 5 2 1 248 1 249 9.23e-180 493
Msa1395330 MtrunA17_Chr5g0444781 51.759 199 95 1 40 238 8 205 1.13e-69 216
Msa1395330 MtrunA17_Chr5g0444781 64.706 51 18 0 40 90 257 307 1.49e-13 68.9
Msa1395330 MtrunA17_Chr6g0483551 48.500 200 101 2 40 238 4 202 1.42e-61 192
Msa1395330 MtrunA17_Chr6g0483591 49.231 195 98 1 44 238 8 201 2.95e-61 192
Msa1395330 MtrunA17_Chr6g0483571 47.980 198 101 2 44 240 8 204 4.06e-60 188
Msa1395330 MtrunA17_Chr3g0103011 41.290 155 90 1 84 238 16 169 5.04e-34 126
Msa1395330 MtrunA17_Chr5g0444791 40.252 159 83 4 84 238 10 160 5.31e-29 109
Msa1395330 MtrunA17_Chr3g0103001 67.442 43 14 0 40 82 35 77 2.09e-14 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1395330 AT4G26485.1 48.529 204 101 3 38 238 4 206 6.23e-59 186
Msa1395330 AT5G56075.1 46.729 214 95 3 31 241 56 253 7.14e-57 182
Msa1395330 AT1G55790.1 42.358 229 114 2 12 240 8 218 1.49e-54 181
Msa1395330 AT5G25030.1 42.857 196 98 2 44 239 11 192 4.43e-48 157
Msa1395330 AT1G55803.1 53.636 110 51 0 41 150 18 127 3.53e-35 122
Msa1395330 AT5G56060.1 35.028 177 100 4 80 244 2 175 1.43e-30 112
Msa1395330 AT2G11045.1 33.121 157 69 4 84 239 14 135 1.32e-16 74.7
Msa1395330 AT1G55800.1 39.773 88 52 1 153 240 10 96 5.52e-14 69.7

Find 77 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTTTAGATCTTCACTTTA+TGG 0.097788 tig0021939:+96541 Msa1395330:three_prime_UTR
CTTTGTTTAGCTAGGGCTTT+TGG 0.226805 tig0021939:+93938 Msa1395330:CDS
AAAGTTGTTGAAGGGGTTTA+TGG 0.239937 tig0021939:+96196 Msa1395330:CDS
ATTCAATGATGCATAGATTA+TGG 0.278424 tig0021939:-93709 None:intergenic
AAGATAGGCTATAATTTGTT+AGG 0.280357 tig0021939:-96470 None:intergenic
TACTTAAACATTGTTGATAA+GGG 0.295882 tig0021939:+96640 Msa1395330:three_prime_UTR
AAACACAAAATTCTCTTTGT+TGG 0.296100 tig0021939:+93893 Msa1395330:CDS
CACTATTCAAGATATTTCTT+TGG 0.318762 tig0021939:-96779 None:intergenic
AAGTTGAAAACTTTATTATA+CGG 0.322819 tig0021939:-93631 None:intergenic
AGCCACTTCCCTTGATTCTC+AGG 0.330415 tig0021939:+93979 Msa1395330:CDS
CTTTCTCTCTTTGTTTAGCT+AGG 0.331146 tig0021939:+93930 Msa1395330:CDS
CATCATTGAACACAGCAAAA+TGG 0.340304 tig0021939:+93852 Msa1395330:CDS
CAGATTTATCTTTCCCTGTT+GGG 0.342684 tig0021939:+96408 Msa1395330:CDS
TAAAGTAACGATTGGTCAAC+AGG 0.343239 tig0021939:+93665 Msa1395330:five_prime_UTR
ACAAGATCCCATTTGTTGTA+TGG 0.352391 tig0021939:-96284 None:intergenic
CTACTTAAACATTGTTGATA+AGG 0.352461 tig0021939:+96639 Msa1395330:three_prime_UTR
AAAGGAAACATTACTGAATC+TGG 0.360922 tig0021939:-94281 None:intergenic
AATCAAGGGAAGTGGCTATA+AGG 0.366752 tig0021939:-93973 None:intergenic
TAAGTACTGGCTTCCCCAAC+AGG 0.374095 tig0021939:-96422 None:intergenic
ATTTGTTAGGGAGAGGTGTT+TGG 0.378150 tig0021939:-96457 None:intergenic
AGGGTGATCCATACAACAAA+TGG 0.390906 tig0021939:+96276 Msa1395330:CDS
TCTTTATGATGTTGATGTTA+AGG 0.404489 tig0021939:+94161 Msa1395330:CDS
CAAAATTCTCTTTGTTGGAG+AGG 0.419240 tig0021939:+93898 Msa1395330:CDS
ATCATCATTGCAGATGAATA+TGG 0.425955 tig0021939:-96753 None:intergenic
TCAGATTTATCTTTCCCTGT+TGG 0.434452 tig0021939:+96407 Msa1395330:CDS
ATCTGGCAGTAACTATTCTC+AGG 0.435024 tig0021939:-94264 None:intergenic
TTTCTCTCTTTGTTTAGCTA+GGG 0.444044 tig0021939:+93931 Msa1395330:CDS
TTAAATAGAGATAAAACATA+TGG 0.452221 tig0021939:-96569 None:intergenic
TTGCATTACCCTGAGAATCA+AGG 0.455310 tig0021939:-93988 None:intergenic
CAAAGAAATATCTTGAATAG+TGG 0.472074 tig0021939:+96780 Msa1395330:three_prime_UTR
GAGCTTGAACTTGTAAGTAC+TGG 0.474156 tig0021939:-96435 None:intergenic
GGCTATAATTTGTTAGGGAG+AGG 0.484720 tig0021939:-96464 None:intergenic
TTCTTTCATAAAGTAACGAT+TGG 0.487363 tig0021939:+93657 Msa1395330:five_prime_UTR
CATTTCCAGAGTTTAGACTT+TGG 0.490874 tig0021939:-96729 None:intergenic
GGATAGTCATCTTTGAAGAA+TGG 0.508198 tig0021939:-96350 None:intergenic
TATGACAACAAGAGAGCTCA+TGG 0.509129 tig0021939:+96377 Msa1395330:CDS
AGATAGGCTATAATTTGTTA+GGG 0.513605 tig0021939:-96469 None:intergenic
ATTACTATTACGTGCGTGTG+TGG 0.514416 tig0021939:+93578 Msa1395330:five_prime_UTR
TTCTTCAAAGATGACTATCC+TGG 0.517775 tig0021939:+96353 Msa1395330:CDS
AAAGAAGTGGGACTAGAAGT+TGG 0.519198 tig0021939:-96495 None:intergenic
AAAATTCTCTTTGTTGGAGA+GGG 0.521483 tig0021939:+93899 Msa1395330:CDS
AGATTTATCTTTCCCTGTTG+GGG 0.524030 tig0021939:+96409 Msa1395330:CDS
ACTAGTAAAGAAAGAGGGAG+GGG 0.524780 tig0021939:+96232 Msa1395330:CDS
AGTAACTATTCTCAGGGTAA+AGG 0.525453 tig0021939:-94257 None:intergenic
ATTGAAAAGAAGTATAGTAA+TGG 0.531936 tig0021939:+94096 Msa1395330:CDS
AAAGGCACTAGTAAAGAAAG+AGG 0.540011 tig0021939:+96226 Msa1395330:CDS
TGCATTACCCTGAGAATCAA+GGG 0.540315 tig0021939:-93987 None:intergenic
GGGTGATCCATACAACAAAT+GGG 0.540448 tig0021939:+96277 Msa1395330:CDS
GCCACTTCCCTTGATTCTCA+GGG 0.542014 tig0021939:+93980 Msa1395330:CDS
TTAGTCCAAAGTCTAAACTC+TGG 0.561802 tig0021939:+96724 Msa1395330:three_prime_UTR
GCAAGGGAACTTGAAGAAAG+AGG 0.562096 tig0021939:+94129 Msa1395330:CDS
ACTTAAACATTGTTGATAAG+GGG 0.562256 tig0021939:+96641 Msa1395330:three_prime_UTR
ACCCTGAGAATCAAGGGAAG+TGG 0.567810 tig0021939:-93981 None:intergenic
ATAAGCCAAAATAAAAGAAG+TGG 0.568651 tig0021939:-96508 None:intergenic
CTTTGAAGAATGGAACAGCT+TGG 0.571652 tig0021939:-96340 None:intergenic
GTAATGGAATGAGTAATGCA+AGG 0.572801 tig0021939:+94112 Msa1395330:CDS
GACTAGAAGTTGGCAAAGAT+AGG 0.577531 tig0021939:-96485 None:intergenic
TTACTATTACGTGCGTGTGT+GGG 0.592682 tig0021939:+93579 Msa1395330:five_prime_UTR
CACTAGTAAAGAAAGAGGGA+GGG 0.592759 tig0021939:+96231 Msa1395330:CDS
GGGGTTTATGGCAAATGCAA+AGG 0.596492 tig0021939:+96208 Msa1395330:CDS
AAGGGGTTTGTAATGAATGA+TGG 0.597722 tig0021939:+96658 Msa1395330:three_prime_UTR
GCTCTCTTGTTGTCATAACC+AGG 0.598483 tig0021939:-96371 None:intergenic
ATTTCTTTCTCAAGACTCAG+AGG 0.603232 tig0021939:+94196 Msa1395330:CDS
TAAGCCAAAATAAAAGAAGT+GGG 0.605418 tig0021939:-96507 None:intergenic
ATTCAAGGTTGAGCTGTGCA+TGG 0.618570 tig0021939:-96805 None:intergenic
TAATGGAATGAGTAATGCAA+GGG 0.623573 tig0021939:+94113 Msa1395330:CDS
TAACGATTGGTCAACAGGGT+CGG 0.624880 tig0021939:+93670 Msa1395330:five_prime_UTR
GCACTAGTAAAGAAAGAGGG+AGG 0.626548 tig0021939:+96230 Msa1395330:CDS
GATTCATGTAACTCACAAAG+AGG 0.650804 tig0021939:+96256 Msa1395330:CDS
GGGTAAAGGAATCCAACATG+AGG 0.659464 tig0021939:-94243 None:intergenic
TCTGGCAGTAACTATTCTCA+GGG 0.659879 tig0021939:-94263 None:intergenic
AAGGCACTAGTAAAGAAAGA+GGG 0.660972 tig0021939:+96227 Msa1395330:CDS
ATTCATGTAACTCACAAAGA+GGG 0.661176 tig0021939:+96257 Msa1395330:CDS
AAGTACTGGCTTCCCCAACA+GGG 0.678090 tig0021939:-96421 None:intergenic
AAAGTAACGATTGGTCAACA+GGG 0.679066 tig0021939:+93666 Msa1395330:five_prime_UTR
TGTGTGGGAGAGAAATAGTG+AGG 0.705020 tig0021939:+93594 Msa1395330:five_prime_UTR
TCAGTGTTAGTGTTAAGTTG+AGG 0.707444 tig0021939:-93782 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAACAAAAATGAAAAA+CGG - tig0021939:94046-94065 None:intergenic 10.0%
!!! ATAATAACATCTTAAAATTT+TGG - tig0021939:94440-94459 None:intergenic 10.0%
!!! TAATAACATCTTAAAATTTT+GGG - tig0021939:94439-94458 None:intergenic 10.0%
!!! ATAATAACATCTTAAAATTT+TGG - tig0021939:94440-94459 None:intergenic 10.0%
!! AAGTTGAAAACTTTATTATA+CGG - tig0021939:93634-93653 None:intergenic 15.0%
!! AAAAATAGTAGTAAATGAAA+AGG - tig0021939:94330-94349 None:intergenic 15.0%
!!! AATAACATCTTAAAATTTTG+GGG - tig0021939:94438-94457 None:intergenic 15.0%
!! TTAAATAGAGATAAAACATA+TGG - tig0021939:96572-96591 None:intergenic 15.0%
!!! ATTGAAAAGAAGTATAGTAA+TGG + tig0021939:94096-94115 Msa1395330:CDS 20.0%
!! AAAAAGTAAAAAAGAGAGAA+AGG - tig0021939:94302-94321 None:intergenic 20.0%
!!! TAACATCTTAAAATTTTGGT+TGG - tig0021939:94436-94455 None:intergenic 20.0%
!!! ATAACATCTTAAAATTTTGG+GGG - tig0021939:94437-94456 None:intergenic 20.0%
!! CTATTTACCCAAAATTTATT+GGG + tig0021939:95002-95021 Msa1395330:intron 20.0%
!!! ATAAATCAGGGTTTTAAAAA+AGG - tig0021939:95381-95400 None:intergenic 20.0%
!!! AAGATAATAACATTTTATCG+AGG - tig0021939:95410-95429 None:intergenic 20.0%
!! TCGATAAAATGTTATTATCT+TGG + tig0021939:95409-95428 Msa1395330:intron 20.0%
!! AATAATCAGAATTTACATTG+CGG - tig0021939:95483-95502 None:intergenic 20.0%
!! TTTCTATTGCTATACTAATT+TGG - tig0021939:95530-95549 None:intergenic 20.0%
!! GTACTTCCTAATAAAATTTA+AGG + tig0021939:95596-95615 Msa1395330:intron 20.0%
!! TTCCTAATAAAATTTAAGGA+TGG + tig0021939:95600-95619 Msa1395330:intron 20.0%
!! TCCTAATAAAATTTAAGGAT+GGG + tig0021939:95601-95620 Msa1395330:intron 20.0%
!! AAAAAAGTAACAGCATAATT+TGG - tig0021939:96596-96615 None:intergenic 20.0%
!!! TACTTTTTTTTTTCTTTCTG+TGG + tig0021939:96607-96626 Msa1395330:three_prime_UTR 20.0%
!!! TACTTAAACATTGTTGATAA+GGG + tig0021939:96640-96659 Msa1395330:three_prime_UTR 20.0%
! TTCTTTCATAAAGTAACGAT+TGG + tig0021939:93657-93676 Msa1395330:five_prime_UTR 25.0%
! ATTCAATGATGCATAGATTA+TGG - tig0021939:93712-93731 None:intergenic 25.0%
! AAACACAAAATTCTCTTTGT+TGG + tig0021939:93893-93912 Msa1395330:CDS 25.0%
! TCTTTATGATGTTGATGTTA+AGG + tig0021939:94161-94180 Msa1395330:CDS 25.0%
!! GTTTATAATTTTCCTCATGT+TGG + tig0021939:94231-94250 Msa1395330:CDS 25.0%
! CTACTTACAAATGTAACTTT+AGG - tig0021939:94366-94385 None:intergenic 25.0%
! CTAAAGTTACATTTGTAAGT+AGG + tig0021939:94364-94383 Msa1395330:intron 25.0%
!!! TCTTATAAGCCAGTTTTTTA+AGG + tig0021939:94399-94418 Msa1395330:intron 25.0%
! ATAGCCTATCCAAAATTTAT+TGG + tig0021939:94458-94477 Msa1395330:intron 25.0%
! TAGCCTATCCAAAATTTATT+GGG + tig0021939:94459-94478 Msa1395330:intron 25.0%
! ATAGCCTATCCAAAATTTAT+TGG + tig0021939:94458-94477 Msa1395330:intron 25.0%
!! TTGTCCAATAAATTTTGGAT+AGG - tig0021939:94624-94643 None:intergenic 25.0%
! TTAACGTTTCAATCTAATGT+GGG - tig0021939:94753-94772 None:intergenic 25.0%
! TAAACATACAAGGACTAAAA+TGG + tig0021939:94979-94998 Msa1395330:intron 25.0%
! GCTATTTACCCAAAATTTAT+TGG + tig0021939:95001-95020 Msa1395330:intron 25.0%
!! ACAATCTATATGTACAGTTT+TGG + tig0021939:95042-95061 Msa1395330:intron 25.0%
!! CAATCTATATGTACAGTTTT+GGG + tig0021939:95043-95062 Msa1395330:intron 25.0%
! CAACCTTGATCTTAAAATTT+GGG - tig0021939:95087-95106 None:intergenic 25.0%
! ACAACCTTGATCTTAAAATT+TGG - tig0021939:95088-95107 None:intergenic 25.0%
! CAAAAATCTGTGTAATATCA+TGG - tig0021939:95138-95157 None:intergenic 25.0%
!! CATGATATTACACAGATTTT+TGG + tig0021939:95136-95155 Msa1395330:intron 25.0%
! AAGGTCACATATACTTAAAT+CGG - tig0021939:95259-95278 None:intergenic 25.0%
! ACTATCTATGTTTAACATCA+AGG - tig0021939:95349-95368 None:intergenic 25.0%
! TTAAACATAGATAGTAATCG+CGG + tig0021939:95354-95373 Msa1395330:intron 25.0%
! TCGAGGATATATATAAATCA+GGG - tig0021939:95393-95412 None:intergenic 25.0%
! ATCGAGGATATATATAAATC+AGG - tig0021939:95394-95413 None:intergenic 25.0%
! AAATGTTATTATCTTGGACT+TGG + tig0021939:95415-95434 Msa1395330:intron 25.0%
! ATAATCAGAATTTACATTGC+GGG - tig0021939:95482-95501 None:intergenic 25.0%
!!! ATACTAATTTGGCTTGATTT+AGG - tig0021939:95519-95538 None:intergenic 25.0%
! GTATAGCAATAGAAATAAGT+AGG + tig0021939:95535-95554 Msa1395330:intron 25.0%
!! TCCCATCCTTAAATTTTATT+AGG - tig0021939:95605-95624 None:intergenic 25.0%
!! AGGTTTGACTAAATTTTACT+CGG + tig0021939:95631-95650 Msa1395330:intron 25.0%
! GTATGAAGAAACAAAAATAG+AGG - tig0021939:95695-95714 None:intergenic 25.0%
! CAAATATTGTCAAATAGTAG+CGG - tig0021939:95940-95959 None:intergenic 25.0%
! TTGAACAAAAAGTTGTTGAA+GGG + tig0021939:96188-96207 Msa1395330:CDS 25.0%
!!! AGATAGGCTATAATTTGTTA+GGG - tig0021939:96472-96491 None:intergenic 25.0%
!!! AAGATAGGCTATAATTTGTT+AGG - tig0021939:96473-96492 None:intergenic 25.0%
! TAAGCCAAAATAAAAGAAGT+GGG - tig0021939:96510-96529 None:intergenic 25.0%
! ATAAGCCAAAATAAAAGAAG+TGG - tig0021939:96511-96530 None:intergenic 25.0%
! ATGTTTAGATCTTCACTTTA+TGG + tig0021939:96541-96560 Msa1395330:three_prime_UTR 25.0%
! CTACTTAAACATTGTTGATA+AGG + tig0021939:96639-96658 Msa1395330:three_prime_UTR 25.0%
!! ACTTAAACATTGTTGATAAG+GGG + tig0021939:96641-96660 Msa1395330:three_prime_UTR 25.0%
! CACTATTCAAGATATTTCTT+TGG - tig0021939:96782-96801 None:intergenic 25.0%
! CAAAGAAATATCTTGAATAG+TGG + tig0021939:96780-96799 Msa1395330:three_prime_UTR 25.0%
!!! TGATCATGTTGTTTTTGTGT+TGG - tig0021939:93761-93780 None:intergenic 30.0%
AAAATTCTCTTTGTTGGAGA+GGG + tig0021939:93899-93918 Msa1395330:CDS 30.0%
! TTTCTCTCTTTGTTTAGCTA+GGG + tig0021939:93931-93950 Msa1395330:CDS 30.0%
AAAAACGGACATGCTAAAAA+AGG - tig0021939:94031-94050 None:intergenic 30.0%
TAATGGAATGAGTAATGCAA+GGG + tig0021939:94113-94132 Msa1395330:CDS 30.0%
AAAGGAAACATTACTGAATC+TGG - tig0021939:94284-94303 None:intergenic 30.0%
CTAAGTCAACCTTAAAAAAC+TGG - tig0021939:94411-94430 None:intergenic 30.0%
!!! CAGTTTTTTAAGGTTGACTT+AGG + tig0021939:94409-94428 Msa1395330:intron 30.0%
! TTGCCCAATAAATTTTGGAT+AGG - tig0021939:94465-94484 None:intergenic 30.0%
! GCTCTTTGTCCAATAAATTT+TGG - tig0021939:94629-94648 None:intergenic 30.0%
TAACGTTTCAATCTAATGTG+GGG - tig0021939:94752-94771 None:intergenic 30.0%
GTTAACGTTTCAATCTAATG+TGG - tig0021939:94754-94773 None:intergenic 30.0%
GTTAACATCTCAATCTTGTT+GGG + tig0021939:94887-94906 Msa1395330:intron 30.0%
TTAACATCTCAATCTTGTTG+GGG + tig0021939:94888-94907 Msa1395330:intron 30.0%
!!! CAACCCAAATTTTAAGATCA+AGG + tig0021939:95081-95100 Msa1395330:intron 30.0%
AGGTCACATATACTTAAATC+GGG - tig0021939:95258-95277 None:intergenic 30.0%
AGCAATAGAAATAAGTAGGT+AGG + tig0021939:95539-95558 Msa1395330:intron 30.0%
ATTTAAGGATGGGAATAGTA+AGG + tig0021939:95611-95630 Msa1395330:intron 30.0%
! GGTTTGACTAAATTTTACTC+GGG + tig0021939:95632-95651 Msa1395330:intron 30.0%
AAAGTTGTACAACAACTTCA+TGG - tig0021939:95791-95810 None:intergenic 30.0%
CGCTACTATTTGACAATATT+TGG + tig0021939:95938-95957 Msa1395330:intron 30.0%
GGTACATAAATACTGTATAG+CGG + tig0021939:95959-95978 Msa1395330:intron 30.0%
GTTGAACAAAAAGTTGTTGA+AGG + tig0021939:96187-96206 Msa1395330:CDS 30.0%
TGAACAAAAAGTTGTTGAAG+GGG + tig0021939:96189-96208 Msa1395330:CDS 30.0%
ATTCATGTAACTCACAAAGA+GGG + tig0021939:96257-96276 Msa1395330:CDS 30.0%
GTAAGAAAAGCTGAAAAAAG+AGG + tig0021939:96305-96324 Msa1395330:CDS 30.0%
!! TAGTCCCACTTCTTTTATTT+TGG + tig0021939:96503-96522 Msa1395330:three_prime_UTR 30.0%
ATCATCATTGCAGATGAATA+TGG - tig0021939:96756-96775 None:intergenic 30.0%
! TAAAGTAACGATTGGTCAAC+AGG + tig0021939:93665-93684 Msa1395330:five_prime_UTR 35.0%
! AAAGTAACGATTGGTCAACA+GGG + tig0021939:93666-93685 Msa1395330:five_prime_UTR 35.0%
! TCAGTGTTAGTGTTAAGTTG+AGG - tig0021939:93785-93804 None:intergenic 35.0%
CATCATTGAACACAGCAAAA+TGG + tig0021939:93852-93871 Msa1395330:CDS 35.0%
CAAAATTCTCTTTGTTGGAG+AGG + tig0021939:93898-93917 Msa1395330:CDS 35.0%
! CTTTCTCTCTTTGTTTAGCT+AGG + tig0021939:93930-93949 Msa1395330:CDS 35.0%
! GTAATGGAATGAGTAATGCA+AGG + tig0021939:94112-94131 Msa1395330:CDS 35.0%
ATTTCTTTCTCAAGACTCAG+AGG + tig0021939:94196-94215 Msa1395330:CDS 35.0%
AGTAACTATTCTCAGGGTAA+AGG - tig0021939:94260-94279 None:intergenic 35.0%
! GCTCTTTGCCCAATAAATTT+TGG - tig0021939:94470-94489 None:intergenic 35.0%
! TATTGGGCAAAGAGCAATTT+TGG + tig0021939:94475-94494 Msa1395330:intron 35.0%
! TATTGGACAAAGAGCAGTTT+TGG + tig0021939:94634-94653 Msa1395330:intron 35.0%
! GTAGGGGATTATTGTGTTTT+TGG - tig0021939:94703-94722 None:intergenic 35.0%
AACGTTTCAATCTAATGTGG+GGG - tig0021939:94751-94770 None:intergenic 35.0%
AGCTTATGTGACATATTGAG+AGG + tig0021939:94786-94805 Msa1395330:intron 35.0%
GGTTAACATCTCAATCTTGT+TGG + tig0021939:94886-94905 Msa1395330:intron 35.0%
! GACTATGACATCAAGGTTTT+TGG - tig0021939:95204-95223 None:intergenic 35.0%
AGTATATGTGACCTTAATCG+TGG + tig0021939:95264-95283 Msa1395330:intron 35.0%
GTAGTTGAATGGCACAAAAA+TGG - tig0021939:95450-95469 None:intergenic 35.0%
GGAAATGCTTAGTAGTTGAA+TGG - tig0021939:95461-95480 None:intergenic 35.0%
TGGCTTGATTTAGGAAATAG+AGG - tig0021939:95510-95529 None:intergenic 35.0%
TTATTGAAAGGAAACCACGA+GGG - tig0021939:95741-95760 None:intergenic 35.0%
TCGTGGTTTCCTTTCAATAA+GGG + tig0021939:95741-95760 Msa1395330:intron 35.0%
TCAAATAGTAGCGGCTATAA+TGG - tig0021939:95931-95950 None:intergenic 35.0%
CTGATCCATGTATTCTTGTT+TGG + tig0021939:96045-96064 Msa1395330:intron 35.0%
ATGCACCAAACAAGAATACA+TGG - tig0021939:96053-96072 None:intergenic 35.0%
!! TATTCTTGTTTGGTGCATTG+TGG + tig0021939:96055-96074 Msa1395330:intron 35.0%
GGAGGAGAAATTACTTTGAA+AGG - tig0021939:96111-96130 None:intergenic 35.0%
! AACTTTTTGTTCAACCTGCT+TGG - tig0021939:96182-96201 None:intergenic 35.0%
AAAGTTGTTGAAGGGGTTTA+TGG + tig0021939:96196-96215 Msa1395330:CDS 35.0%
!! AAAGGCACTAGTAAAGAAAG+AGG + tig0021939:96226-96245 Msa1395330:CDS 35.0%
! AAGGCACTAGTAAAGAAAGA+GGG + tig0021939:96227-96246 Msa1395330:CDS 35.0%
GATTCATGTAACTCACAAAG+AGG + tig0021939:96256-96275 Msa1395330:CDS 35.0%
!! ACAAGATCCCATTTGTTGTA+TGG - tig0021939:96287-96306 None:intergenic 35.0%
! GGATAGTCATCTTTGAAGAA+TGG - tig0021939:96353-96372 None:intergenic 35.0%
TTCTTCAAAGATGACTATCC+TGG + tig0021939:96353-96372 Msa1395330:CDS 35.0%
TCAGATTTATCTTTCCCTGT+TGG + tig0021939:96407-96426 Msa1395330:CDS 35.0%
CAGATTTATCTTTCCCTGTT+GGG + tig0021939:96408-96427 Msa1395330:CDS 35.0%
AGATTTATCTTTCCCTGTTG+GGG + tig0021939:96409-96428 Msa1395330:CDS 35.0%
AAGGGGTTTGTAATGAATGA+TGG + tig0021939:96658-96677 Msa1395330:three_prime_UTR 35.0%
TTAGTCCAAAGTCTAAACTC+TGG + tig0021939:96724-96743 Msa1395330:three_prime_UTR 35.0%
CATTTCCAGAGTTTAGACTT+TGG - tig0021939:96732-96751 None:intergenic 35.0%
ATTACTATTACGTGCGTGTG+TGG + tig0021939:93578-93597 Msa1395330:five_prime_UTR 40.0%
TTACTATTACGTGCGTGTGT+GGG + tig0021939:93579-93598 Msa1395330:five_prime_UTR 40.0%
!! CTTTGTTTAGCTAGGGCTTT+TGG + tig0021939:93938-93957 Msa1395330:CDS 40.0%
AATCAAGGGAAGTGGCTATA+AGG - tig0021939:93976-93995 None:intergenic 40.0%
TGCATTACCCTGAGAATCAA+GGG - tig0021939:93990-94009 None:intergenic 40.0%
TTGCATTACCCTGAGAATCA+AGG - tig0021939:93991-94010 None:intergenic 40.0%
TCTGGCAGTAACTATTCTCA+GGG - tig0021939:94266-94285 None:intergenic 40.0%
ATCTGGCAGTAACTATTCTC+AGG - tig0021939:94267-94286 None:intergenic 40.0%
!! CAAAGAGCAATTTTGGTCCT+TGG + tig0021939:94482-94501 Msa1395330:intron 40.0%
TAACAGACTGCAAAGTGAGT+GGG - tig0021939:94562-94581 None:intergenic 40.0%
! TTTGCTTTCATTTTCGTCCC+TGG - tig0021939:94834-94853 None:intergenic 40.0%
GACAGTGAGGTAAACATACA+AGG + tig0021939:94969-94988 Msa1395330:intron 40.0%
! TGGGTGGCCCAATAAATTTT+GGG - tig0021939:95012-95031 None:intergenic 40.0%
ATAGATTGTGACCTGAGAGT+GGG - tig0021939:95031-95050 None:intergenic 40.0%
TATAGATTGTGACCTGAGAG+TGG - tig0021939:95032-95051 None:intergenic 40.0%
TGAGATTAACGTCGCATCAA+CGG - tig0021939:95169-95188 None:intergenic 40.0%
AAACCTTGATGTCATAGTCC+AGG + tig0021939:95205-95224 Msa1395330:intron 40.0%
AAATGTCTATGACCACAACC+TGG - tig0021939:95226-95245 None:intergenic 40.0%
ACACAATGTGACCTTAATCG+TGG + tig0021939:95300-95319 Msa1395330:intron 40.0%
CTTATTGAAAGGAAACCACG+AGG - tig0021939:95742-95761 None:intergenic 40.0%
CTCGTGGTTTCCTTTCAATA+AGG + tig0021939:95740-95759 Msa1395330:intron 40.0%
CGTGGTTTCCTTTCAATAAG+GGG + tig0021939:95742-95761 Msa1395330:intron 40.0%
TCCTTTCAATAAGGGGTTCT+TGG + tig0021939:95749-95768 Msa1395330:intron 40.0%
TCCAAGAACCCCTTATTGAA+AGG - tig0021939:95753-95772 None:intergenic 40.0%
! TCCATCAGTGTCGTTAATTG+TGG + tig0021939:95852-95871 Msa1395330:intron 40.0%
! CCATCAGTGTCGTTAATTGT+GGG + tig0021939:95853-95872 Msa1395330:intron 40.0%
TTGTGGGCAGCAGAAAATAT+CGG + tig0021939:95869-95888 Msa1395330:intron 40.0%
TACACTGTCTCAATAGATGG+AGG - tig0021939:96129-96148 None:intergenic 40.0%
CCATACACTGTCTCAATAGA+TGG - tig0021939:96132-96151 None:intergenic 40.0%
CCATCTATTGAGACAGTGTA+TGG + tig0021939:96129-96148 Msa1395330:intron 40.0%
TGAATTTGTGACAACCAAGC+AGG + tig0021939:96165-96184 Msa1395330:intron 40.0%
CACTAGTAAAGAAAGAGGGA+GGG + tig0021939:96231-96250 Msa1395330:CDS 40.0%
ACTAGTAAAGAAAGAGGGAG+GGG + tig0021939:96232-96251 Msa1395330:CDS 40.0%
AGGGTGATCCATACAACAAA+TGG + tig0021939:96276-96295 Msa1395330:CDS 40.0%
GGGTGATCCATACAACAAAT+GGG + tig0021939:96277-96296 Msa1395330:CDS 40.0%
! CTTTGAAGAATGGAACAGCT+TGG - tig0021939:96343-96362 None:intergenic 40.0%
TATGACAACAAGAGAGCTCA+TGG + tig0021939:96377-96396 Msa1395330:CDS 40.0%
GAGCTTGAACTTGTAAGTAC+TGG - tig0021939:96438-96457 None:intergenic 40.0%
!! ATTTGTTAGGGAGAGGTGTT+TGG - tig0021939:96460-96479 None:intergenic 40.0%
!! GGCTATAATTTGTTAGGGAG+AGG - tig0021939:96467-96486 None:intergenic 40.0%
GACTAGAAGTTGGCAAAGAT+AGG - tig0021939:96488-96507 None:intergenic 40.0%
AAAGAAGTGGGACTAGAAGT+TGG - tig0021939:96498-96517 None:intergenic 40.0%
TGTGTGGGAGAGAAATAGTG+AGG + tig0021939:93594-93613 Msa1395330:five_prime_UTR 45.0%
! TAACGATTGGTCAACAGGGT+CGG + tig0021939:93670-93689 Msa1395330:five_prime_UTR 45.0%
GCAAGGGAACTTGAAGAAAG+AGG + tig0021939:94129-94148 Msa1395330:CDS 45.0%
GGGTAAAGGAATCCAACATG+AGG - tig0021939:94246-94265 None:intergenic 45.0%
TGTCATAGTAGTCCCTGACT+CGG + tig0021939:94518-94537 Msa1395330:intron 45.0%
!!! TTGTGTTTTTGGCCGAGTCA+GGG - tig0021939:94533-94552 None:intergenic 45.0%
!!! ATTGTGTTTTTGGCCGAGTC+AGG - tig0021939:94534-94553 None:intergenic 45.0%
! GTGGGGGGTTATTGTGTTTT+TGG - tig0021939:94544-94563 None:intergenic 45.0%
AACAGACTGCAAAGTGAGTG+GGG - tig0021939:94561-94580 None:intergenic 45.0%
CTAACAGACTGCAAAGTGAG+TGG - tig0021939:94563-94582 None:intergenic 45.0%
!! CAAAGAGCAGTTTTGGTCCT+TGG + tig0021939:94641-94660 Msa1395330:intron 45.0%
TGTCATAGTAGTCCCTGACT+CGG + tig0021939:94518-94537 Msa1395330:intron 45.0%
!!! TTGTGTTTTTGGCCGAGTCA+GGG - tig0021939:94533-94552 None:intergenic 45.0%
!!! ATTGTGTTTTTGGCCGAGTC+AGG - tig0021939:94534-94553 None:intergenic 45.0%
TGACATATTGAGAGGCCAGT+AGG + tig0021939:94794-94813 Msa1395330:intron 45.0%
CAAATCCCACTTTGTCACGT+CGG + tig0021939:94850-94869 Msa1395330:intron 45.0%
ATGACCAACAGAATGCAGAG+TGG + tig0021939:94917-94936 Msa1395330:intron 45.0%
TGACCAACAGAATGCAGAGT+GGG + tig0021939:94918-94937 Msa1395330:intron 45.0%
TCAGGACTACTATGACAGTG+AGG + tig0021939:94956-94975 Msa1395330:intron 45.0%
! GTGGGTGGCCCAATAAATTT+TGG - tig0021939:95013-95032 None:intergenic 45.0%
CAACCTGGACTATGACATCA+AGG - tig0021939:95211-95230 None:intergenic 45.0%
TGATGTCATAGTCCAGGTTG+TGG + tig0021939:95211-95230 Msa1395330:intron 45.0%
TTGCAGCACAACCACGATTA+AGG - tig0021939:95278-95297 None:intergenic 45.0%
CAATAAGGGGTTCTTGGAAC+TGG + tig0021939:95755-95774 Msa1395330:intron 45.0%
CCCACAATTAACGACACTGA+TGG - tig0021939:95856-95875 None:intergenic 45.0%
!! GTTTGGTGCATTGTGGACTA+TGG + tig0021939:96062-96081 Msa1395330:intron 45.0%
GGGGTTTATGGCAAATGCAA+AGG + tig0021939:96208-96227 Msa1395330:CDS 45.0%
GCACTAGTAAAGAAAGAGGG+AGG + tig0021939:96230-96249 Msa1395330:CDS 45.0%
! GCTCTCTTGTTGTCATAACC+AGG - tig0021939:96374-96393 None:intergenic 45.0%
AGCCACTTCCCTTGATTCTC+AGG + tig0021939:93979-93998 Msa1395330:CDS 50.0%
GCCACTTCCCTTGATTCTCA+GGG + tig0021939:93980-93999 Msa1395330:CDS 50.0%
ACCCTGAGAATCAAGGGAAG+TGG - tig0021939:93984-94003 None:intergenic 50.0%
GACATCGAGGTACACATCCA+AGG - tig0021939:94502-94521 None:intergenic 50.0%
CAGGGACTACTATGACATCG+AGG - tig0021939:94515-94534 None:intergenic 50.0%
ACAGACTGCAAAGTGAGTGG+GGG - tig0021939:94560-94579 None:intergenic 50.0%
GACATCGAGGTACACATCCA+AGG - tig0021939:94502-94521 None:intergenic 50.0%
CAGGGACTACTATGACATCG+AGG - tig0021939:94515-94534 None:intergenic 50.0%
!! ACGAACGGACTGCAAAGTAG+GGG - tig0021939:94719-94738 None:intergenic 50.0%
!! GACGAACGGACTGCAAAGTA+GGG - tig0021939:94720-94739 None:intergenic 50.0%
!! TGACGAACGGACTGCAAAGT+AGG - tig0021939:94721-94740 None:intergenic 50.0%
TGGGGGACTACTATGACGAA+CGG - tig0021939:94734-94753 None:intergenic 50.0%
TATTGAGAGGCCAGTAGGCA+TGG + tig0021939:94799-94818 Msa1395330:intron 50.0%
GTACACTTCACCATGCCTAC+TGG - tig0021939:94812-94831 None:intergenic 50.0%
TAGGCATGGTGAAGTGTACC+AGG + tig0021939:94813-94832 Msa1395330:intron 50.0%
AGGCATGGTGAAGTGTACCA+GGG + tig0021939:94814-94833 Msa1395330:intron 50.0%
! CACGTCGGCTGACAAATCAT+CGG + tig0021939:94865-94884 Msa1395330:intron 50.0%
GACCAACAGAATGCAGAGTG+GGG + tig0021939:94919-94938 Msa1395330:intron 50.0%
ACCAACAGAATGCAGAGTGG+GGG + tig0021939:94920-94939 Msa1395330:intron 50.0%
AATGCAGAGTGGGGGGTTAT+TGG + tig0021939:94928-94947 Msa1395330:intron 50.0%
TTGCAGCGCAACCACGATTA+AGG - tig0021939:95314-95333 None:intergenic 50.0%
ATGAACCAAGTTAGCCCTCG+TGG + tig0021939:95724-95743 Msa1395330:intron 50.0%
!! AAGTACTGGCTTCCCCAACA+GGG - tig0021939:96424-96443 None:intergenic 50.0%
!! TAAGTACTGGCTTCCCCAAC+AGG - tig0021939:96425-96444 None:intergenic 50.0%
CAGACTGCAAAGTGAGTGGG+GGG - tig0021939:94559-94578 None:intergenic 55.0%
GTCAGCCGACGTGACAAAGT+GGG - tig0021939:94858-94877 None:intergenic 55.0%
TGTCAGCCGACGTGACAAAG+TGG - tig0021939:94859-94878 None:intergenic 55.0%
CCAACAGAATGCAGAGTGGG+GGG + tig0021939:94921-94940 Msa1395330:intron 55.0%
!! GGGGGGTTATTGGCTCAATC+AGG + tig0021939:94938-94957 Msa1395330:intron 55.0%
TTATTGGGCCACCCACTCTC+AGG + tig0021939:95017-95036 Msa1395330:intron 55.0%
GATTGTGACCTGAGAGTGGG+TGG - tig0021939:95028-95047 None:intergenic 55.0%
GGAAACCACGAGGGCTAACT+TGG - tig0021939:95732-95751 None:intergenic 55.0%
CCCCCCACTCTGCATTCTGT+TGG - tig0021939:94924-94943 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0021939 gene 93546 96814 93546 ID=Msa1395330;Name=Msa1395330
tig0021939 mRNA 93546 96814 93546 ID=Msa1395330-mRNA-1;Parent=Msa1395330;Name=Msa1395330-mRNA-1;_AED=0.00;_eAED=0.00;_QI=206|1|1|1|1|1|3|330|248
tig0021939 exon 93546 94001 93546 ID=Msa1395330-mRNA-1:exon:14806;Parent=Msa1395330-mRNA-1
tig0021939 exon 94091 94289 94091 ID=Msa1395330-mRNA-1:exon:14807;Parent=Msa1395330-mRNA-1
tig0021939 exon 96187 96814 96187 ID=Msa1395330-mRNA-1:exon:14808;Parent=Msa1395330-mRNA-1
tig0021939 five_prime_UTR 93546 93751 93546 ID=Msa1395330-mRNA-1:five_prime_utr;Parent=Msa1395330-mRNA-1
tig0021939 CDS 93752 94001 93752 ID=Msa1395330-mRNA-1:cds;Parent=Msa1395330-mRNA-1
tig0021939 CDS 94091 94289 94091 ID=Msa1395330-mRNA-1:cds;Parent=Msa1395330-mRNA-1
tig0021939 CDS 96187 96484 96187 ID=Msa1395330-mRNA-1:cds;Parent=Msa1395330-mRNA-1
tig0021939 three_prime_UTR 96485 96814 96485 ID=Msa1395330-mRNA-1:three_prime_utr;Parent=Msa1395330-mRNA-1
Gene Sequence

>Msa1395330

ATGAGTCCAACACAAAAACAACATGATCATCCTCAACTTAACACTAACACTGACACTGATGATGAAGAACAAGAATCAGAATCAGAGTCAGAAGAAGAATCATCATTGAACACAGCAAAATGGAAAAAACACTACTCATCAAAACACAAAATTCTCTTTGTTGGAGAGGGTGATTTTTCTTTCTCTCTTTGTTTAGCTAGGGCTTTTGGTTCTGCTCATAACCTTATAGCCACTTCCCTTGATTCTCAGGAGAAAATTGAAAAGAAGTATAGTAATGGAATGAGTAATGCAAGGGAACTTGAAGAAAGAGGTTGTATTGTTCTTTATGATGTTGATGTTAAGGTGATGAGTCAGCATTTCTTTCTCAAGACTCAGAGGTTTGATCGTGTTGTTTATAATTTTCCTCATGTTGGATTCCTTTACCCTGAGAATAGTTACTGCCAGATTCAGTTGAACAAAAAGTTGTTGAAGGGGTTTATGGCAAATGCAAAGGCACTAGTAAAGAAAGAGGGAGGGGAGATTCATGTAACTCACAAAGAGGGTGATCCATACAACAAATGGGATCTTGTAAGAAAAGCTGAAAAAAGAGGACTGTTCTTACACCAAGCTGTTCCATTCTTCAAAGATGACTATCCTGGTTATGACAACAAGAGAGCTCATGGAAAGTTATCAGATTTATCTTTCCCTGTTGGGGAAGCCAGTACTTACAAGTTCAAGCTCCAAACACCTCTCCCTAACAAATTATAG
Protein sequence

>Msa1395330

MSPTQKQHDHPQLNTNTDTDDEEQESESESEEESSLNTAKWKKHYSSKHKILFVGEGDFSFSLCLARAFGSAHNLIATSLDSQEKIEKKYSNGMSNARELEERGCIVLYDVDVKVMSQHFFLKTQRFDRVVYNFPHVGFLYPENSYCQIQLNKKLLKGFMANAKALVKKEGGEIHVTHKEGDPYNKWDLVRKAEKRGLFLHQAVPFFKDDYPGYDNKRAHGKLSDLSFPVGEASTYKFKLQTPLPNKL*