AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0197690

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0197690 MtrunA17_Chr2g0290801 96.800 250 5 2 1 248 1 249 9.23e-180 493
Msa0197690 MtrunA17_Chr5g0444781 51.759 199 95 1 40 238 8 205 1.13e-69 216
Msa0197690 MtrunA17_Chr5g0444781 64.706 51 18 0 40 90 257 307 1.49e-13 68.9
Msa0197690 MtrunA17_Chr6g0483551 48.500 200 101 2 40 238 4 202 1.42e-61 192
Msa0197690 MtrunA17_Chr6g0483591 49.231 195 98 1 44 238 8 201 2.95e-61 192
Msa0197690 MtrunA17_Chr6g0483571 47.980 198 101 2 44 240 8 204 4.06e-60 188
Msa0197690 MtrunA17_Chr3g0103011 41.290 155 90 1 84 238 16 169 5.04e-34 126
Msa0197690 MtrunA17_Chr5g0444791 40.252 159 83 4 84 238 10 160 5.31e-29 109
Msa0197690 MtrunA17_Chr3g0103001 67.442 43 14 0 40 82 35 77 2.09e-14 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0197690 AT4G26485.1 48.529 204 101 3 38 238 4 206 6.23e-59 186
Msa0197690 AT5G56075.1 46.729 214 95 3 31 241 56 253 7.14e-57 182
Msa0197690 AT1G55790.1 42.358 229 114 2 12 240 8 218 1.49e-54 181
Msa0197690 AT5G25030.1 42.857 196 98 2 44 239 11 192 4.43e-48 157
Msa0197690 AT1G55803.1 53.636 110 51 0 41 150 18 127 3.53e-35 122
Msa0197690 AT5G56060.1 35.028 177 100 4 80 244 2 175 1.43e-30 112
Msa0197690 AT2G11045.1 33.121 157 69 4 84 239 14 135 1.32e-16 74.7
Msa0197690 AT1G55800.1 39.773 88 52 1 153 240 10 96 5.52e-14 69.7

Find 77 sgRNAs with CRISPR-Local

Find 240 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTTTAGATCTTCACTTTA+TGG 0.097788 2_1:-15867287 Msa0197690:three_prime_UTR
CTTTGTTTAGCTAGGGCTTT+TGG 0.226805 2_1:-15869885 Msa0197690:CDS
AAAGTTGTTGAAGGGGTTTA+TGG 0.239937 2_1:-15867632 Msa0197690:CDS
ATTCAATGATGCATAGATTA+TGG 0.278424 2_1:+15870114 None:intergenic
AAGATAGGCTATAATTTGTT+AGG 0.280357 2_1:+15867358 None:intergenic
TACTTAAACATTGTTGATAA+GGG 0.295882 2_1:-15867187 Msa0197690:three_prime_UTR
AAACACAAAATTCTCTTTGT+TGG 0.296100 2_1:-15869930 Msa0197690:CDS
CACTATTCAAGATATTTCTT+TGG 0.318762 2_1:+15867048 None:intergenic
AAGTTGAAAACTTTATTATA+CGG 0.322819 2_1:+15870192 None:intergenic
AGCCACTTCCCTTGATTCTC+AGG 0.330415 2_1:-15869844 Msa0197690:CDS
CTTTCTCTCTTTGTTTAGCT+AGG 0.331146 2_1:-15869893 Msa0197690:CDS
CATCATTGAACACAGCAAAA+TGG 0.340304 2_1:-15869971 Msa0197690:CDS
CAGATTTATCTTTCCCTGTT+GGG 0.342684 2_1:-15867420 Msa0197690:CDS
TAAAGTAACGATTGGTCAAC+AGG 0.343239 2_1:-15870158 Msa0197690:five_prime_UTR
ACAAGATCCCATTTGTTGTA+TGG 0.352391 2_1:+15867544 None:intergenic
CTACTTAAACATTGTTGATA+AGG 0.352461 2_1:-15867188 Msa0197690:three_prime_UTR
AAAGGAAACATTACTGAATC+TGG 0.360922 2_1:+15869542 None:intergenic
AATCAAGGGAAGTGGCTATA+AGG 0.366752 2_1:+15869850 None:intergenic
TAAGTACTGGCTTCCCCAAC+AGG 0.374095 2_1:+15867406 None:intergenic
ATTTGTTAGGGAGAGGTGTT+TGG 0.378150 2_1:+15867371 None:intergenic
AGGGTGATCCATACAACAAA+TGG 0.390906 2_1:-15867552 Msa0197690:CDS
TCTTTATGATGTTGATGTTA+AGG 0.404489 2_1:-15869662 Msa0197690:CDS
CAAAATTCTCTTTGTTGGAG+AGG 0.419240 2_1:-15869925 Msa0197690:CDS
ATCATCATTGCAGATGAATA+TGG 0.425955 2_1:+15867074 None:intergenic
TCAGATTTATCTTTCCCTGT+TGG 0.434452 2_1:-15867421 Msa0197690:CDS
ATCTGGCAGTAACTATTCTC+AGG 0.435024 2_1:+15869559 None:intergenic
TTTCTCTCTTTGTTTAGCTA+GGG 0.444044 2_1:-15869892 Msa0197690:CDS
TTAAATAGAGATAAAACATA+TGG 0.452221 2_1:+15867259 None:intergenic
TTGCATTACCCTGAGAATCA+AGG 0.455310 2_1:+15869835 None:intergenic
CAAAGAAATATCTTGAATAG+TGG 0.472074 2_1:-15867047 Msa0197690:three_prime_UTR
GAGCTTGAACTTGTAAGTAC+TGG 0.474156 2_1:+15867393 None:intergenic
GGCTATAATTTGTTAGGGAG+AGG 0.484720 2_1:+15867364 None:intergenic
TTCTTTCATAAAGTAACGAT+TGG 0.487363 2_1:-15870166 Msa0197690:five_prime_UTR
CATTTCCAGAGTTTAGACTT+TGG 0.490874 2_1:+15867098 None:intergenic
GGATAGTCATCTTTGAAGAA+TGG 0.508198 2_1:+15867478 None:intergenic
TATGACAACAAGAGAGCTCA+TGG 0.509129 2_1:-15867451 Msa0197690:CDS
AGATAGGCTATAATTTGTTA+GGG 0.513605 2_1:+15867359 None:intergenic
ATTACTATTACGTGCGTGTG+TGG 0.514416 2_1:-15870245 Msa0197690:five_prime_UTR
TTCTTCAAAGATGACTATCC+TGG 0.517775 2_1:-15867475 Msa0197690:CDS
AAAGAAGTGGGACTAGAAGT+TGG 0.519198 2_1:+15867333 None:intergenic
AAAATTCTCTTTGTTGGAGA+GGG 0.521483 2_1:-15869924 Msa0197690:CDS
AGATTTATCTTTCCCTGTTG+GGG 0.524030 2_1:-15867419 Msa0197690:CDS
ACTAGTAAAGAAAGAGGGAG+GGG 0.524780 2_1:-15867596 Msa0197690:CDS
AGTAACTATTCTCAGGGTAA+AGG 0.525453 2_1:+15869566 None:intergenic
ATTGAAAAGAAGTATAGTAA+TGG 0.531936 2_1:-15869727 Msa0197690:CDS
AAAGGCACTAGTAAAGAAAG+AGG 0.540011 2_1:-15867602 Msa0197690:CDS
TGCATTACCCTGAGAATCAA+GGG 0.540315 2_1:+15869836 None:intergenic
GGGTGATCCATACAACAAAT+GGG 0.540448 2_1:-15867551 Msa0197690:CDS
GCCACTTCCCTTGATTCTCA+GGG 0.542014 2_1:-15869843 Msa0197690:intron
TTAGTCCAAAGTCTAAACTC+TGG 0.561802 2_1:-15867103 Msa0197690:three_prime_UTR
GCAAGGGAACTTGAAGAAAG+AGG 0.562096 2_1:-15869694 Msa0197690:CDS
ACTTAAACATTGTTGATAAG+GGG 0.562256 2_1:-15867186 Msa0197690:three_prime_UTR
ACCCTGAGAATCAAGGGAAG+TGG 0.567810 2_1:+15869842 None:intergenic
ATAAGCCAAAATAAAAGAAG+TGG 0.568651 2_1:+15867320 None:intergenic
CTTTGAAGAATGGAACAGCT+TGG 0.571652 2_1:+15867488 None:intergenic
GTAATGGAATGAGTAATGCA+AGG 0.572801 2_1:-15869711 Msa0197690:CDS
GACTAGAAGTTGGCAAAGAT+AGG 0.577531 2_1:+15867343 None:intergenic
TTACTATTACGTGCGTGTGT+GGG 0.592682 2_1:-15870244 Msa0197690:five_prime_UTR
CACTAGTAAAGAAAGAGGGA+GGG 0.592759 2_1:-15867597 Msa0197690:CDS
GGGGTTTATGGCAAATGCAA+AGG 0.596492 2_1:-15867620 Msa0197690:CDS
AAGGGGTTTGTAATGAATGA+TGG 0.597722 2_1:-15867169 Msa0197690:three_prime_UTR
GCTCTCTTGTTGTCATAACC+AGG 0.598483 2_1:+15867457 None:intergenic
ATTTCTTTCTCAAGACTCAG+AGG 0.603232 2_1:-15869627 Msa0197690:CDS
TAAGCCAAAATAAAAGAAGT+GGG 0.605418 2_1:+15867321 None:intergenic
ATTCAAGGTTGAGCTGTGCA+TGG 0.618570 2_1:+15867022 None:intergenic
TAATGGAATGAGTAATGCAA+GGG 0.623573 2_1:-15869710 Msa0197690:CDS
TAACGATTGGTCAACAGGGT+CGG 0.624880 2_1:-15870153 Msa0197690:five_prime_UTR
GCACTAGTAAAGAAAGAGGG+AGG 0.626548 2_1:-15867598 Msa0197690:CDS
GATTCATGTAACTCACAAAG+AGG 0.650804 2_1:-15867572 Msa0197690:CDS
GGGTAAAGGAATCCAACATG+AGG 0.659464 2_1:+15869580 None:intergenic
TCTGGCAGTAACTATTCTCA+GGG 0.659879 2_1:+15869560 None:intergenic
AAGGCACTAGTAAAGAAAGA+GGG 0.660972 2_1:-15867601 Msa0197690:CDS
ATTCATGTAACTCACAAAGA+GGG 0.661176 2_1:-15867571 Msa0197690:CDS
AAGTACTGGCTTCCCCAACA+GGG 0.678090 2_1:+15867407 None:intergenic
AAAGTAACGATTGGTCAACA+GGG 0.679066 2_1:-15870157 Msa0197690:five_prime_UTR
TGTGTGGGAGAGAAATAGTG+AGG 0.705020 2_1:-15870229 Msa0197690:five_prime_UTR
TCAGTGTTAGTGTTAAGTTG+AGG 0.707444 2_1:+15870041 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAACAAAAATGAAAAA+CGG + chr2_1:15867535-15867554 None:intergenic 10.0%
!!! ATAATAACATCTTAAAATTT+TGG + chr2_1:15867928-15867947 None:intergenic 10.0%
!!! TAATAACATCTTAAAATTTT+GGG + chr2_1:15867927-15867946 None:intergenic 10.0%
!!! ATAATAACATCTTAAAATTT+TGG + chr2_1:15867928-15867947 None:intergenic 10.0%
!! AAGTTGAAAACTTTATTATA+CGG + chr2_1:15867123-15867142 None:intergenic 15.0%
!! AAAAATAGTAGTAAATGAAA+AGG + chr2_1:15867818-15867837 None:intergenic 15.0%
!!! AATAACATCTTAAAATTTTG+GGG + chr2_1:15867926-15867945 None:intergenic 15.0%
!! TTAAATAGAGATAAAACATA+TGG + chr2_1:15870056-15870075 None:intergenic 15.0%
!!! ATTGAAAAGAAGTATAGTAA+TGG - chr2_1:15867585-15867604 Msa0197690:CDS 20.0%
!! AAAAAGTAAAAAAGAGAGAA+AGG + chr2_1:15867791-15867810 None:intergenic 20.0%
!!! TAACATCTTAAAATTTTGGT+TGG + chr2_1:15867924-15867943 None:intergenic 20.0%
!!! ATAACATCTTAAAATTTTGG+GGG + chr2_1:15867925-15867944 None:intergenic 20.0%
!!! ATAAATCAGGGTTTTAAAAA+AGG + chr2_1:15868865-15868884 None:intergenic 20.0%
!!! AAGATAATAACATTTTATCG+AGG + chr2_1:15868894-15868913 None:intergenic 20.0%
!! TCGATAAAATGTTATTATCT+TGG - chr2_1:15868893-15868912 Msa0197690:intron 20.0%
!! AATAATCAGAATTTACATTG+CGG + chr2_1:15868967-15868986 None:intergenic 20.0%
!! TTTCTATTGCTATACTAATT+TGG + chr2_1:15869014-15869033 None:intergenic 20.0%
!! GTACTTCCTAATAAAATTTA+AGG - chr2_1:15869080-15869099 Msa0197690:intron 20.0%
!! TTCCTAATAAAATTTAAGGA+TGG - chr2_1:15869084-15869103 Msa0197690:intron 20.0%
!! TCCTAATAAAATTTAAGGAT+GGG - chr2_1:15869085-15869104 Msa0197690:intron 20.0%
!! AAAAAAGTAACAGCATAATT+TGG + chr2_1:15870080-15870099 None:intergenic 20.0%
!!! ACTTTTTTTTTTTCTTTCTG+TGG - chr2_1:15870092-15870111 Msa0197690:exon 20.0%
!!! TACTTAAACATTGTTGATAA+GGG - chr2_1:15870125-15870144 Msa0197690:five_prime_UTR 20.0%
! TTCTTTCATAAAGTAACGAT+TGG - chr2_1:15867146-15867165 Msa0197690:three_prime_UTR 25.0%
! ATTCAATGATGCATAGATTA+TGG + chr2_1:15867201-15867220 None:intergenic 25.0%
! AAACACAAAATTCTCTTTGT+TGG - chr2_1:15867382-15867401 Msa0197690:CDS 25.0%
! TCTTTATGATGTTGATGTTA+AGG - chr2_1:15867650-15867669 Msa0197690:intron 25.0%
!! GTTTATAATTTTCCTCATGT+TGG - chr2_1:15867720-15867739 Msa0197690:intron 25.0%
! CTACTTACAAATGTAACTTT+AGG + chr2_1:15867854-15867873 None:intergenic 25.0%
! CTAAAGTTACATTTGTAAGT+AGG - chr2_1:15867852-15867871 Msa0197690:intron 25.0%
!!! TCTTATAAGCCAGTTTTTTA+AGG - chr2_1:15867887-15867906 Msa0197690:intron 25.0%
! ATAGCCTATCCAAAATTTAT+TGG - chr2_1:15867946-15867965 Msa0197690:intron 25.0%
! TAGCCTATCCAAAATTTATT+GGG - chr2_1:15867947-15867966 Msa0197690:intron 25.0%
! ATAGCCTATCCAAAATTTAT+TGG - chr2_1:15867946-15867965 Msa0197690:intron 25.0%
!! TTGTCCAATAAATTTTGGAT+AGG + chr2_1:15868111-15868130 None:intergenic 25.0%
! TTAACGTTTCAATCTAATGT+GGG + chr2_1:15868239-15868258 None:intergenic 25.0%
! TAAACATACAAGGACTAAAA+TGG - chr2_1:15868464-15868483 Msa0197690:intron 25.0%
! GCTATTTACCCAAATTTATT+GGG - chr2_1:15868486-15868505 Msa0197690:intron 25.0%
!! ACAATCTATATGTACAGTTT+TGG - chr2_1:15868526-15868545 Msa0197690:intron 25.0%
!! CAATCTATATGTACAGTTTT+GGG - chr2_1:15868527-15868546 Msa0197690:intron 25.0%
! CAACCTTGATCTTAAAATTT+GGG + chr2_1:15868571-15868590 None:intergenic 25.0%
! ACAACCTTGATCTTAAAATT+TGG + chr2_1:15868572-15868591 None:intergenic 25.0%
! CAAAAATCTGTGTAATATCA+TGG + chr2_1:15868622-15868641 None:intergenic 25.0%
!! CATGATATTACACAGATTTT+TGG - chr2_1:15868620-15868639 Msa0197690:intron 25.0%
! AAGGTCACATATACTTAAAT+CGG + chr2_1:15868743-15868762 None:intergenic 25.0%
! ACTATCTATGTTTAACATCA+AGG + chr2_1:15868833-15868852 None:intergenic 25.0%
! TTAAACATAGATAGTAATCG+CGG - chr2_1:15868838-15868857 Msa0197690:intron 25.0%
! TCGAGGATATATATAAATCA+GGG + chr2_1:15868877-15868896 None:intergenic 25.0%
! ATCGAGGATATATATAAATC+AGG + chr2_1:15868878-15868897 None:intergenic 25.0%
! AAATGTTATTATCTTGGACT+TGG - chr2_1:15868899-15868918 Msa0197690:intron 25.0%
! ATAATCAGAATTTACATTGC+GGG + chr2_1:15868966-15868985 None:intergenic 25.0%
!!! ATACTAATTTGGCTTGATTT+AGG + chr2_1:15869003-15869022 None:intergenic 25.0%
! GTATAGCAATAGAAATAAGT+AGG - chr2_1:15869019-15869038 Msa0197690:intron 25.0%
!! TCCCATCCTTAAATTTTATT+AGG + chr2_1:15869089-15869108 None:intergenic 25.0%
!! AGGTTTGACTAAATTTTACT+CGG - chr2_1:15869115-15869134 Msa0197690:intron 25.0%
! GTATGAAGAAACAAAAATAG+AGG + chr2_1:15869179-15869198 None:intergenic 25.0%
! CAAATATTGTCAAATAGTAG+CGG + chr2_1:15869424-15869443 None:intergenic 25.0%
! TTGAACAAAAAGTTGTTGAA+GGG - chr2_1:15869672-15869691 Msa0197690:CDS 25.0%
!!! AGATAGGCTATAATTTGTTA+GGG + chr2_1:15869956-15869975 None:intergenic 25.0%
!!! AAGATAGGCTATAATTTGTT+AGG + chr2_1:15869957-15869976 None:intergenic 25.0%
! TAAGCCAAAATAAAAGAAGT+GGG + chr2_1:15869994-15870013 None:intergenic 25.0%
! ATAAGCCAAAATAAAAGAAG+TGG + chr2_1:15869995-15870014 None:intergenic 25.0%
! ATGTTTAGATCTTCACTTTA+TGG - chr2_1:15870025-15870044 Msa0197690:CDS 25.0%
! CTACTTAAACATTGTTGATA+AGG - chr2_1:15870124-15870143 Msa0197690:five_prime_UTR 25.0%
!! ACTTAAACATTGTTGATAAG+GGG - chr2_1:15870126-15870145 Msa0197690:five_prime_UTR 25.0%
! CACTATTCAAGATATTTCTT+TGG + chr2_1:15870267-15870286 None:intergenic 25.0%
! CAAAGAAATATCTTGAATAG+TGG - chr2_1:15870265-15870284 Msa0197690:five_prime_UTR 25.0%
!!! TGATCATGTTGTTTTTGTGT+TGG + chr2_1:15867250-15867269 None:intergenic 30.0%
AAAATTCTCTTTGTTGGAGA+GGG - chr2_1:15867388-15867407 Msa0197690:CDS 30.0%
! TTTCTCTCTTTGTTTAGCTA+GGG - chr2_1:15867420-15867439 Msa0197690:CDS 30.0%
AAAAACGGACATGCTAAAAA+AGG + chr2_1:15867520-15867539 None:intergenic 30.0%
TAATGGAATGAGTAATGCAA+GGG - chr2_1:15867602-15867621 Msa0197690:CDS 30.0%
AAAGGAAACATTACTGAATC+TGG + chr2_1:15867773-15867792 None:intergenic 30.0%
CTAAGTCAACCTTAAAAAAC+TGG + chr2_1:15867899-15867918 None:intergenic 30.0%
!!! CAGTTTTTTAAGGTTGACTT+AGG - chr2_1:15867897-15867916 Msa0197690:intron 30.0%
! TTGCCCAATAAATTTTGGAT+AGG + chr2_1:15867953-15867972 None:intergenic 30.0%
! GCTCTTTGTCCAATAAATTT+TGG + chr2_1:15868116-15868135 None:intergenic 30.0%
TAACGTTTCAATCTAATGTG+GGG + chr2_1:15868238-15868257 None:intergenic 30.0%
GTTAACGTTTCAATCTAATG+TGG + chr2_1:15868240-15868259 None:intergenic 30.0%
GTTAACATCTCAATCTTGTT+GGG - chr2_1:15868373-15868392 Msa0197690:intron 30.0%
TTAACATCTCAATCTTGTTG+GGG - chr2_1:15868374-15868393 Msa0197690:intron 30.0%
GGCTATTTACCCAAATTTAT+TGG - chr2_1:15868485-15868504 Msa0197690:intron 30.0%
!!! CAACCCAAATTTTAAGATCA+AGG - chr2_1:15868565-15868584 Msa0197690:intron 30.0%
AGGTCACATATACTTAAATC+GGG + chr2_1:15868742-15868761 None:intergenic 30.0%
AGCAATAGAAATAAGTAGGT+AGG - chr2_1:15869023-15869042 Msa0197690:intron 30.0%
ATTTAAGGATGGGAATAGTA+AGG - chr2_1:15869095-15869114 Msa0197690:intron 30.0%
! GGTTTGACTAAATTTTACTC+GGG - chr2_1:15869116-15869135 Msa0197690:intron 30.0%
AAAGTTGTACAACAACTTCA+TGG + chr2_1:15869275-15869294 None:intergenic 30.0%
CGCTACTATTTGACAATATT+TGG - chr2_1:15869422-15869441 Msa0197690:intron 30.0%
GGTACATAAATACTGTATAG+CGG - chr2_1:15869443-15869462 Msa0197690:intron 30.0%
GTTGAACAAAAAGTTGTTGA+AGG - chr2_1:15869671-15869690 Msa0197690:CDS 30.0%
TGAACAAAAAGTTGTTGAAG+GGG - chr2_1:15869673-15869692 Msa0197690:CDS 30.0%
ATTCATGTAACTCACAAAGA+GGG - chr2_1:15869741-15869760 Msa0197690:intron 30.0%
GTAAGAAAAGCTGAAAAAAG+AGG - chr2_1:15869789-15869808 Msa0197690:intron 30.0%
!! TAGTCCCACTTCTTTTATTT+TGG - chr2_1:15869987-15870006 Msa0197690:CDS 30.0%
ATCATCATTGCAGATGAATA+TGG + chr2_1:15870241-15870260 None:intergenic 30.0%
! TAAAGTAACGATTGGTCAAC+AGG - chr2_1:15867154-15867173 Msa0197690:three_prime_UTR 35.0%
! AAAGTAACGATTGGTCAACA+GGG - chr2_1:15867155-15867174 Msa0197690:three_prime_UTR 35.0%
! TCAGTGTTAGTGTTAAGTTG+AGG + chr2_1:15867274-15867293 None:intergenic 35.0%
CATCATTGAACACAGCAAAA+TGG - chr2_1:15867341-15867360 Msa0197690:three_prime_UTR 35.0%
CAAAATTCTCTTTGTTGGAG+AGG - chr2_1:15867387-15867406 Msa0197690:CDS 35.0%
! CTTTCTCTCTTTGTTTAGCT+AGG - chr2_1:15867419-15867438 Msa0197690:CDS 35.0%
! GTAATGGAATGAGTAATGCA+AGG - chr2_1:15867601-15867620 Msa0197690:CDS 35.0%
ATTTCTTTCTCAAGACTCAG+AGG - chr2_1:15867685-15867704 Msa0197690:intron 35.0%
AGTAACTATTCTCAGGGTAA+AGG + chr2_1:15867749-15867768 None:intergenic 35.0%
! GCTCTTTGCCCAATAAATTT+TGG + chr2_1:15867958-15867977 None:intergenic 35.0%
! TATTGGGCAAAGAGCAATTT+TGG - chr2_1:15867963-15867982 Msa0197690:intron 35.0%
! TATTGGACAAAGAGCAGTTT+TGG - chr2_1:15868121-15868140 Msa0197690:intron 35.0%
! GTAGGGGATTATTGTGTTTT+TGG + chr2_1:15868190-15868209 None:intergenic 35.0%
AGCTTATGTGACATATTGAG+AGG - chr2_1:15868272-15868291 Msa0197690:intron 35.0%
GGTTAACATCTCAATCTTGT+TGG - chr2_1:15868372-15868391 Msa0197690:intron 35.0%
! GACTATGACATCAAGGTTTT+TGG + chr2_1:15868688-15868707 None:intergenic 35.0%
AGTATATGTGACCTTAATCG+TGG - chr2_1:15868748-15868767 Msa0197690:intron 35.0%
GTAGTTGAATGGCACAAAAA+TGG + chr2_1:15868934-15868953 None:intergenic 35.0%
GGAAATGCTTAGTAGTTGAA+TGG + chr2_1:15868945-15868964 None:intergenic 35.0%
TGGCTTGATTTAGGAAATAG+AGG + chr2_1:15868994-15869013 None:intergenic 35.0%
TTATTGAAAGGAAACCACGA+GGG + chr2_1:15869225-15869244 None:intergenic 35.0%
TCGTGGTTTCCTTTCAATAA+GGG - chr2_1:15869225-15869244 Msa0197690:intron 35.0%
TCAAATAGTAGCGGCTATAA+TGG + chr2_1:15869415-15869434 None:intergenic 35.0%
CTGATCCATGTATTCTTGTT+TGG - chr2_1:15869529-15869548 Msa0197690:intron 35.0%
ATGCACCAAACAAGAATACA+TGG + chr2_1:15869537-15869556 None:intergenic 35.0%
!! TATTCTTGTTTGGTGCATTG+TGG - chr2_1:15869539-15869558 Msa0197690:intron 35.0%
GGAGGAGAAATTACTTTGAA+AGG + chr2_1:15869595-15869614 None:intergenic 35.0%
! AACTTTTTGTTCAACCTGCT+TGG + chr2_1:15869666-15869685 None:intergenic 35.0%
AAAGTTGTTGAAGGGGTTTA+TGG - chr2_1:15869680-15869699 Msa0197690:CDS 35.0%
!! AAAGGCACTAGTAAAGAAAG+AGG - chr2_1:15869710-15869729 Msa0197690:CDS 35.0%
! AAGGCACTAGTAAAGAAAGA+GGG - chr2_1:15869711-15869730 Msa0197690:CDS 35.0%
GATTCATGTAACTCACAAAG+AGG - chr2_1:15869740-15869759 Msa0197690:intron 35.0%
!! ACAAGATCCCATTTGTTGTA+TGG + chr2_1:15869771-15869790 None:intergenic 35.0%
! GGATAGTCATCTTTGAAGAA+TGG + chr2_1:15869837-15869856 None:intergenic 35.0%
TTCTTCAAAGATGACTATCC+TGG - chr2_1:15869837-15869856 Msa0197690:intron 35.0%
TCAGATTTATCTTTCCCTGT+TGG - chr2_1:15869891-15869910 Msa0197690:CDS 35.0%
CAGATTTATCTTTCCCTGTT+GGG - chr2_1:15869892-15869911 Msa0197690:CDS 35.0%
AGATTTATCTTTCCCTGTTG+GGG - chr2_1:15869893-15869912 Msa0197690:CDS 35.0%
AAGGGGTTTGTAATGAATGA+TGG - chr2_1:15870143-15870162 Msa0197690:five_prime_UTR 35.0%
TTAGTCCAAAGTCTAAACTC+TGG - chr2_1:15870209-15870228 Msa0197690:five_prime_UTR 35.0%
CATTTCCAGAGTTTAGACTT+TGG + chr2_1:15870217-15870236 None:intergenic 35.0%
ATTACTATTACGTGCGTGTG+TGG - chr2_1:15867067-15867086 Msa0197690:three_prime_UTR 40.0%
TTACTATTACGTGCGTGTGT+GGG - chr2_1:15867068-15867087 Msa0197690:three_prime_UTR 40.0%
!! CTTTGTTTAGCTAGGGCTTT+TGG - chr2_1:15867427-15867446 Msa0197690:CDS 40.0%
AATCAAGGGAAGTGGCTATA+AGG + chr2_1:15867465-15867484 None:intergenic 40.0%
TGCATTACCCTGAGAATCAA+GGG + chr2_1:15867479-15867498 None:intergenic 40.0%
TTGCATTACCCTGAGAATCA+AGG + chr2_1:15867480-15867499 None:intergenic 40.0%
TCTGGCAGTAACTATTCTCA+GGG + chr2_1:15867755-15867774 None:intergenic 40.0%
ATCTGGCAGTAACTATTCTC+AGG + chr2_1:15867756-15867775 None:intergenic 40.0%
!! CAAAGAGCAATTTTGGTCCT+TGG - chr2_1:15867970-15867989 Msa0197690:intron 40.0%
TAACAGACTGCAAAGTGAGT+GGG + chr2_1:15868049-15868068 None:intergenic 40.0%
! TTTGCTTTCATTTTCGTCCC+TGG + chr2_1:15868320-15868339 None:intergenic 40.0%
GACAGTGAGGTAAACATACA+AGG - chr2_1:15868454-15868473 Msa0197690:intron 40.0%
ATAGATTGTGACCTGAGAGT+GGG + chr2_1:15868515-15868534 None:intergenic 40.0%
TATAGATTGTGACCTGAGAG+TGG + chr2_1:15868516-15868535 None:intergenic 40.0%
TGAGATTAACGTCGCATCAA+CGG + chr2_1:15868653-15868672 None:intergenic 40.0%
AAACCTTGATGTCATAGTCC+AGG - chr2_1:15868689-15868708 Msa0197690:intron 40.0%
AAATGTCTATGACCACAACC+TGG + chr2_1:15868710-15868729 None:intergenic 40.0%
ACACAATGTGACCTTAATCG+TGG - chr2_1:15868784-15868803 Msa0197690:intron 40.0%
CTTATTGAAAGGAAACCACG+AGG + chr2_1:15869226-15869245 None:intergenic 40.0%
CTCGTGGTTTCCTTTCAATA+AGG - chr2_1:15869224-15869243 Msa0197690:intron 40.0%
CGTGGTTTCCTTTCAATAAG+GGG - chr2_1:15869226-15869245 Msa0197690:intron 40.0%
TCCTTTCAATAAGGGGTTCT+TGG - chr2_1:15869233-15869252 Msa0197690:intron 40.0%
TCCAAGAACCCCTTATTGAA+AGG + chr2_1:15869237-15869256 None:intergenic 40.0%
! TCCATCAGTGTCGTTAATTG+TGG - chr2_1:15869336-15869355 Msa0197690:intron 40.0%
! CCATCAGTGTCGTTAATTGT+GGG - chr2_1:15869337-15869356 Msa0197690:intron 40.0%
TTGTGGGCAGCAGAAAATAT+CGG - chr2_1:15869353-15869372 Msa0197690:intron 40.0%
TACACTGTCTCAATAGATGG+AGG + chr2_1:15869613-15869632 None:intergenic 40.0%
CCATACACTGTCTCAATAGA+TGG + chr2_1:15869616-15869635 None:intergenic 40.0%
CCATCTATTGAGACAGTGTA+TGG - chr2_1:15869613-15869632 Msa0197690:CDS 40.0%
TGAATTTGTGACAACCAAGC+AGG - chr2_1:15869649-15869668 Msa0197690:CDS 40.0%
CACTAGTAAAGAAAGAGGGA+GGG - chr2_1:15869715-15869734 Msa0197690:CDS 40.0%
ACTAGTAAAGAAAGAGGGAG+GGG - chr2_1:15869716-15869735 Msa0197690:CDS 40.0%
AGGGTGATCCATACAACAAA+TGG - chr2_1:15869760-15869779 Msa0197690:intron 40.0%
GGGTGATCCATACAACAAAT+GGG - chr2_1:15869761-15869780 Msa0197690:intron 40.0%
! CTTTGAAGAATGGAACAGCT+TGG + chr2_1:15869827-15869846 None:intergenic 40.0%
TATGACAACAAGAGAGCTCA+TGG - chr2_1:15869861-15869880 Msa0197690:CDS 40.0%
GAGCTTGAACTTGTAAGTAC+TGG + chr2_1:15869922-15869941 None:intergenic 40.0%
!! ATTTGTTAGGGAGAGGTGTT+TGG + chr2_1:15869944-15869963 None:intergenic 40.0%
!! GGCTATAATTTGTTAGGGAG+AGG + chr2_1:15869951-15869970 None:intergenic 40.0%
GACTAGAAGTTGGCAAAGAT+AGG + chr2_1:15869972-15869991 None:intergenic 40.0%
AAAGAAGTGGGACTAGAAGT+TGG + chr2_1:15869982-15870001 None:intergenic 40.0%
TGTGTGGGAGAGAAATAGTG+AGG - chr2_1:15867083-15867102 Msa0197690:three_prime_UTR 45.0%
! TAACGATTGGTCAACAGGGT+CGG - chr2_1:15867159-15867178 Msa0197690:three_prime_UTR 45.0%
GCAAGGGAACTTGAAGAAAG+AGG - chr2_1:15867618-15867637 Msa0197690:CDS 45.0%
GGGTAAAGGAATCCAACATG+AGG + chr2_1:15867735-15867754 None:intergenic 45.0%
TGTCATAGTAGTCCCTGACT+CGG - chr2_1:15868006-15868025 Msa0197690:intron 45.0%
!!! ATTGTGTTTTGGCCGAGTCA+GGG + chr2_1:15868021-15868040 None:intergenic 45.0%
!!! TATTGTGTTTTGGCCGAGTC+AGG + chr2_1:15868022-15868041 None:intergenic 45.0%
! AGTGGGGGGTTATTGTGTTT+TGG + chr2_1:15868032-15868051 None:intergenic 45.0%
AACAGACTGCAAAGTGAGTG+GGG + chr2_1:15868048-15868067 None:intergenic 45.0%
CTAACAGACTGCAAAGTGAG+TGG + chr2_1:15868050-15868069 None:intergenic 45.0%
!! CAAAGAGCAGTTTTGGTCCT+TGG - chr2_1:15868128-15868147 Msa0197690:intron 45.0%
TGTCATAGTAGTCCCTGACT+CGG - chr2_1:15868006-15868025 Msa0197690:intron 45.0%
!!! TTGTGTTTTTGGCCGAGTCA+GGG + chr2_1:15868179-15868198 None:intergenic 45.0%
!!! ATTGTGTTTTTGGCCGAGTC+AGG + chr2_1:15868180-15868199 None:intergenic 45.0%
TGACATATTGAGAGGCCAGT+AGG - chr2_1:15868280-15868299 Msa0197690:intron 45.0%
CAAATCCCACTTTGTCACGT+CGG - chr2_1:15868336-15868355 Msa0197690:intron 45.0%
ATGACCAACAGAATGCAGAG+TGG - chr2_1:15868403-15868422 Msa0197690:intron 45.0%
TGACCAACAGAATGCAGAGT+GGG - chr2_1:15868404-15868423 Msa0197690:intron 45.0%
TCAGGACTACTATGACAGTG+AGG - chr2_1:15868441-15868460 Msa0197690:intron 45.0%
GTGGGTGGCCCAATAAATTT+GGG + chr2_1:15868497-15868516 None:intergenic 45.0%
AGTGGGTGGCCCAATAAATT+TGG + chr2_1:15868498-15868517 None:intergenic 45.0%
CAACCTGGACTATGACATCA+AGG + chr2_1:15868695-15868714 None:intergenic 45.0%
TGATGTCATAGTCCAGGTTG+TGG - chr2_1:15868695-15868714 Msa0197690:intron 45.0%
TTGCAGCACAACCACGATTA+AGG + chr2_1:15868762-15868781 None:intergenic 45.0%
CAATAAGGGGTTCTTGGAAC+TGG - chr2_1:15869239-15869258 Msa0197690:intron 45.0%
CCCACAATTAACGACACTGA+TGG + chr2_1:15869340-15869359 None:intergenic 45.0%
!! GTTTGGTGCATTGTGGACTA+TGG - chr2_1:15869546-15869565 Msa0197690:intron 45.0%
GGGGTTTATGGCAAATGCAA+AGG - chr2_1:15869692-15869711 Msa0197690:CDS 45.0%
GCACTAGTAAAGAAAGAGGG+AGG - chr2_1:15869714-15869733 Msa0197690:CDS 45.0%
! GCTCTCTTGTTGTCATAACC+AGG + chr2_1:15869858-15869877 None:intergenic 45.0%
AGCCACTTCCCTTGATTCTC+AGG - chr2_1:15867468-15867487 Msa0197690:CDS 50.0%
GCCACTTCCCTTGATTCTCA+GGG - chr2_1:15867469-15867488 Msa0197690:CDS 50.0%
ACCCTGAGAATCAAGGGAAG+TGG + chr2_1:15867473-15867492 None:intergenic 50.0%
GACATCGAGGTACACATCCA+AGG + chr2_1:15867990-15868009 None:intergenic 50.0%
CAGGGACTACTATGACATCG+AGG + chr2_1:15868003-15868022 None:intergenic 50.0%
ACAGACTGCAAAGTGAGTGG+GGG + chr2_1:15868047-15868066 None:intergenic 50.0%
GACATCGAGGTACACATCCA+AGG + chr2_1:15867990-15868009 None:intergenic 50.0%
CAGGGACTACTATGACATCG+AGG + chr2_1:15868003-15868022 None:intergenic 50.0%
!! ACGAACGGACTGCAAAGTAG+GGG + chr2_1:15868206-15868225 None:intergenic 50.0%
!! GACGAACGGACTGCAAAGTA+GGG + chr2_1:15868207-15868226 None:intergenic 50.0%
!! TGACGAACGGACTGCAAAGT+AGG + chr2_1:15868208-15868227 None:intergenic 50.0%
GTGGGGACTACTATGACGAA+CGG + chr2_1:15868221-15868240 None:intergenic 50.0%
TATTGAGAGGCCAGTAGGCA+TGG - chr2_1:15868285-15868304 Msa0197690:intron 50.0%
GTACACTTCACCATGCCTAC+TGG + chr2_1:15868298-15868317 None:intergenic 50.0%
TAGGCATGGTGAAGTGTACC+AGG - chr2_1:15868299-15868318 Msa0197690:intron 50.0%
AGGCATGGTGAAGTGTACCA+GGG - chr2_1:15868300-15868319 Msa0197690:intron 50.0%
! CACGTCGGCTGACAAATCAT+CGG - chr2_1:15868351-15868370 Msa0197690:intron 50.0%
GACCAACAGAATGCAGAGTG+GGG - chr2_1:15868405-15868424 Msa0197690:intron 50.0%
ACCAACAGAATGCAGAGTGG+GGG - chr2_1:15868406-15868425 Msa0197690:intron 50.0%
GAATGCAGAGTGGGGGTTAT+TGG - chr2_1:15868413-15868432 Msa0197690:intron 50.0%
!! TGGGGGTTATTGGCTCAATC+AGG - chr2_1:15868423-15868442 Msa0197690:intron 50.0%
TTGCAGCGCAACCACGATTA+AGG + chr2_1:15868798-15868817 None:intergenic 50.0%
ATGAACCAAGTTAGCCCTCG+TGG - chr2_1:15869208-15869227 Msa0197690:intron 50.0%
!! AAGTACTGGCTTCCCCAACA+GGG + chr2_1:15869908-15869927 None:intergenic 50.0%
!! TAAGTACTGGCTTCCCCAAC+AGG + chr2_1:15869909-15869928 None:intergenic 50.0%
CAGACTGCAAAGTGAGTGGG+GGG + chr2_1:15868046-15868065 None:intergenic 55.0%
GTCAGCCGACGTGACAAAGT+GGG + chr2_1:15868344-15868363 None:intergenic 55.0%
TGTCAGCCGACGTGACAAAG+TGG + chr2_1:15868345-15868364 None:intergenic 55.0%
ACCCCCACTCTGCATTCTGT+TGG + chr2_1:15868410-15868429 None:intergenic 55.0%
TTATTGGGCCACCCACTCTC+AGG - chr2_1:15868501-15868520 Msa0197690:intron 55.0%
GATTGTGACCTGAGAGTGGG+TGG + chr2_1:15868512-15868531 None:intergenic 55.0%
GGAAACCACGAGGGCTAACT+TGG + chr2_1:15869216-15869235 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr2_1 gene 15867035 15870299 15867035 ID=Msa0197690;Name=Msa0197690
chr2_1 mRNA 15867035 15870299 15867035 ID=Msa0197690-mRNA-1;Parent=Msa0197690;Name=Msa0197690-mRNA-1;_AED=0.00;_eAED=0.00;_QI=206|1|1|1|1|1|3|331|248
chr2_1 exon 15867035 15867663 15867035 ID=Msa0197690-mRNA-1:exon:6611;Parent=Msa0197690-mRNA-1
chr2_1 exon 15869556 15869754 15869556 ID=Msa0197690-mRNA-1:exon:6610;Parent=Msa0197690-mRNA-1
chr2_1 exon 15869844 15870299 15869844 ID=Msa0197690-mRNA-1:exon:6609;Parent=Msa0197690-mRNA-1
chr2_1 five_prime_UTR 15870094 15870299 15870094 ID=Msa0197690-mRNA-1:five_prime_utr;Parent=Msa0197690-mRNA-1
chr2_1 CDS 15869844 15870093 15869844 ID=Msa0197690-mRNA-1:cds;Parent=Msa0197690-mRNA-1
chr2_1 CDS 15869556 15869754 15869556 ID=Msa0197690-mRNA-1:cds;Parent=Msa0197690-mRNA-1
chr2_1 CDS 15867366 15867663 15867366 ID=Msa0197690-mRNA-1:cds;Parent=Msa0197690-mRNA-1
chr2_1 three_prime_UTR 15867035 15867365 15867035 ID=Msa0197690-mRNA-1:three_prime_utr;Parent=Msa0197690-mRNA-1
Gene Sequence

>Msa0197690

ATGAGTCCAACACAAAAACAACATGATCATCCTCAACTTAACACTAACACTGACACTGATGATGAAGAACAAGAATCAGAATCAGAGTCAGAAGAAGAATCATCATTGAACACAGCAAAATGGAAAAAACACTACTCATCAAAACACAAAATTCTCTTTGTTGGAGAGGGTGATTTTTCTTTCTCTCTTTGTTTAGCTAGGGCTTTTGGTTCTGCTCATAACCTTATAGCCACTTCCCTTGATTCTCAGGAGAAAATTGAAAAGAAGTATAGTAATGGAATGAGTAATGCAAGGGAACTTGAAGAAAGAGGTTGTATTGTTCTTTATGATGTTGATGTTAAGGTGATGAGTCAGCATTTCTTTCTCAAGACTCAGAGGTTTGATCGTGTTGTTTATAATTTTCCTCATGTTGGATTCCTTTACCCTGAGAATAGTTACTGCCAGATTCAGTTGAACAAAAAGTTGTTGAAGGGGTTTATGGCAAATGCAAAGGCACTAGTAAAGAAAGAGGGAGGGGAGATTCATGTAACTCACAAAGAGGGTGATCCATACAACAAATGGGATCTTGTAAGAAAAGCTGAAAAAAGAGGACTGTTCTTACACCAAGCTGTTCCATTCTTCAAAGATGACTATCCTGGTTATGACAACAAGAGAGCTCATGGAAAGTTATCAGATTTATCTTTCCCTGTTGGGGAAGCCAGTACTTACAAGTTCAAGCTCCAAACACCTCTCCCTAACAAATTATAG
Protein sequence

>Msa0197690

MSPTQKQHDHPQLNTNTDTDDEEQESESESEEESSLNTAKWKKHYSSKHKILFVGEGDFSFSLCLARAFGSAHNLIATSLDSQEKIEKKYSNGMSNARELEERGCIVLYDVDVKVMSQHFFLKTQRFDRVVYNFPHVGFLYPENSYCQIQLNKKLLKGFMANAKALVKKEGGEIHVTHKEGDPYNKWDLVRKAEKRGLFLHQAVPFFKDDYPGYDNKRAHGKLSDLSFPVGEASTYKFKLQTPLPNKL*