AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0239770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0239770 MtrunA17_Chr2g0290801 96.800 250 5 2 1 248 1 249 9.23e-180 493
Msa0239770 MtrunA17_Chr5g0444781 51.759 199 95 1 40 238 8 205 1.13e-69 216
Msa0239770 MtrunA17_Chr5g0444781 64.706 51 18 0 40 90 257 307 1.49e-13 68.9
Msa0239770 MtrunA17_Chr6g0483551 48.500 200 101 2 40 238 4 202 1.42e-61 192
Msa0239770 MtrunA17_Chr6g0483591 49.231 195 98 1 44 238 8 201 2.95e-61 192
Msa0239770 MtrunA17_Chr6g0483571 47.980 198 101 2 44 240 8 204 4.06e-60 188
Msa0239770 MtrunA17_Chr3g0103011 41.290 155 90 1 84 238 16 169 5.04e-34 126
Msa0239770 MtrunA17_Chr5g0444791 40.252 159 83 4 84 238 10 160 5.31e-29 109
Msa0239770 MtrunA17_Chr3g0103001 67.442 43 14 0 40 82 35 77 2.09e-14 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0239770 AT4G26485.1 48.529 204 101 3 38 238 4 206 6.23e-59 186
Msa0239770 AT5G56075.1 46.729 214 95 3 31 241 56 253 7.14e-57 182
Msa0239770 AT1G55790.1 42.358 229 114 2 12 240 8 218 1.49e-54 181
Msa0239770 AT5G25030.1 42.857 196 98 2 44 239 11 192 4.43e-48 157
Msa0239770 AT1G55803.1 53.636 110 51 0 41 150 18 127 3.53e-35 122
Msa0239770 AT5G56060.1 35.028 177 100 4 80 244 2 175 1.43e-30 112
Msa0239770 AT2G11045.1 33.121 157 69 4 84 239 14 135 1.32e-16 74.7
Msa0239770 AT1G55800.1 39.773 88 52 1 153 240 10 96 5.52e-14 69.7

Find 77 sgRNAs with CRISPR-Local

Find 242 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTTTAGATCTTCACTTTA+TGG 0.097788 2_2:+14355655 Msa0239770:three_prime_UTR
CTTTGTTTAGCTAGGGCTTT+TGG 0.226805 2_2:+14353052 Msa0239770:CDS
AAAGTTGTTGAAGGGGTTTA+TGG 0.239937 2_2:+14355310 Msa0239770:CDS
ATTCAATGATGCATAGATTA+TGG 0.278424 2_2:-14352823 None:intergenic
AAGATAGGCTATAATTTGTT+AGG 0.280357 2_2:-14355584 None:intergenic
TACTTAAACATTGTTGATAA+GGG 0.295882 2_2:+14355755 Msa0239770:three_prime_UTR
AAACACAAAATTCTCTTTGT+TGG 0.296100 2_2:+14353007 Msa0239770:CDS
CACTATTCAAGATATTTCTT+TGG 0.318762 2_2:-14355894 None:intergenic
AAGTTGAAAACTTTATTATA+CGG 0.322819 2_2:-14352745 None:intergenic
AGCCACTTCCCTTGATTCTC+AGG 0.330415 2_2:+14353093 Msa0239770:CDS
CTTTCTCTCTTTGTTTAGCT+AGG 0.331146 2_2:+14353044 Msa0239770:CDS
CATCATTGAACACAGCAAAA+TGG 0.340304 2_2:+14352966 Msa0239770:CDS
CAGATTTATCTTTCCCTGTT+GGG 0.342684 2_2:+14355522 Msa0239770:CDS
TAAAGTAACGATTGGTCAAC+AGG 0.343239 2_2:+14352779 Msa0239770:five_prime_UTR
ACAAGATCCCATTTGTTGTA+TGG 0.352391 2_2:-14355398 None:intergenic
CTACTTAAACATTGTTGATA+AGG 0.352461 2_2:+14355754 Msa0239770:three_prime_UTR
AAAGGAAACATTACTGAATC+TGG 0.360922 2_2:-14353395 None:intergenic
AATCAAGGGAAGTGGCTATA+AGG 0.366752 2_2:-14353087 None:intergenic
TAAGTACTGGCTTCCCCAAC+AGG 0.374095 2_2:-14355536 None:intergenic
ATTTGTTAGGGAGAGGTGTT+TGG 0.378150 2_2:-14355571 None:intergenic
AGGGTGATCCATACAACAAA+TGG 0.390906 2_2:+14355390 Msa0239770:CDS
TCTTTATGATGTTGATGTTA+AGG 0.404489 2_2:+14353275 Msa0239770:CDS
CAAAATTCTCTTTGTTGGAG+AGG 0.419240 2_2:+14353012 Msa0239770:CDS
ATCATCATTGCAGATGAATA+TGG 0.425955 2_2:-14355868 None:intergenic
TCAGATTTATCTTTCCCTGT+TGG 0.434452 2_2:+14355521 Msa0239770:CDS
ATCTGGCAGTAACTATTCTC+AGG 0.435024 2_2:-14353378 None:intergenic
TTTCTCTCTTTGTTTAGCTA+GGG 0.444044 2_2:+14353045 Msa0239770:CDS
TTAAATAGAGATAAAACATA+TGG 0.452221 2_2:-14355683 None:intergenic
TTGCATTACCCTGAGAATCA+AGG 0.455310 2_2:-14353102 None:intergenic
CAAAGAAATATCTTGAATAG+TGG 0.472074 2_2:+14355895 Msa0239770:three_prime_UTR
GAGCTTGAACTTGTAAGTAC+TGG 0.474156 2_2:-14355549 None:intergenic
GGCTATAATTTGTTAGGGAG+AGG 0.484720 2_2:-14355578 None:intergenic
TTCTTTCATAAAGTAACGAT+TGG 0.487363 2_2:+14352771 Msa0239770:five_prime_UTR
CATTTCCAGAGTTTAGACTT+TGG 0.490874 2_2:-14355844 None:intergenic
GGATAGTCATCTTTGAAGAA+TGG 0.508198 2_2:-14355464 None:intergenic
TATGACAACAAGAGAGCTCA+TGG 0.509129 2_2:+14355491 Msa0239770:CDS
AGATAGGCTATAATTTGTTA+GGG 0.513605 2_2:-14355583 None:intergenic
ATTACTATTACGTGCGTGTG+TGG 0.514416 2_2:+14352692 Msa0239770:five_prime_UTR
TTCTTCAAAGATGACTATCC+TGG 0.517775 2_2:+14355467 Msa0239770:CDS
AAAGAAGTGGGACTAGAAGT+TGG 0.519198 2_2:-14355609 None:intergenic
AAAATTCTCTTTGTTGGAGA+GGG 0.521483 2_2:+14353013 Msa0239770:CDS
AGATTTATCTTTCCCTGTTG+GGG 0.524030 2_2:+14355523 Msa0239770:CDS
ACTAGTAAAGAAAGAGGGAG+GGG 0.524780 2_2:+14355346 Msa0239770:CDS
AGTAACTATTCTCAGGGTAA+AGG 0.525453 2_2:-14353371 None:intergenic
ATTGAAAAGAAGTATAGTAA+TGG 0.531936 2_2:+14353210 Msa0239770:CDS
AAAGGCACTAGTAAAGAAAG+AGG 0.540011 2_2:+14355340 Msa0239770:CDS
TGCATTACCCTGAGAATCAA+GGG 0.540315 2_2:-14353101 None:intergenic
GGGTGATCCATACAACAAAT+GGG 0.540448 2_2:+14355391 Msa0239770:CDS
GCCACTTCCCTTGATTCTCA+GGG 0.542014 2_2:+14353094 Msa0239770:CDS
TTAGTCCAAAGTCTAAACTC+TGG 0.561802 2_2:+14355839 Msa0239770:three_prime_UTR
GCAAGGGAACTTGAAGAAAG+AGG 0.562096 2_2:+14353243 Msa0239770:CDS
ACTTAAACATTGTTGATAAG+GGG 0.562256 2_2:+14355756 Msa0239770:three_prime_UTR
ACCCTGAGAATCAAGGGAAG+TGG 0.567810 2_2:-14353095 None:intergenic
ATAAGCCAAAATAAAAGAAG+TGG 0.568651 2_2:-14355622 None:intergenic
CTTTGAAGAATGGAACAGCT+TGG 0.571652 2_2:-14355454 None:intergenic
GTAATGGAATGAGTAATGCA+AGG 0.572801 2_2:+14353226 Msa0239770:CDS
GACTAGAAGTTGGCAAAGAT+AGG 0.577531 2_2:-14355599 None:intergenic
TTACTATTACGTGCGTGTGT+GGG 0.592682 2_2:+14352693 Msa0239770:five_prime_UTR
CACTAGTAAAGAAAGAGGGA+GGG 0.592759 2_2:+14355345 Msa0239770:CDS
GGGGTTTATGGCAAATGCAA+AGG 0.596492 2_2:+14355322 Msa0239770:CDS
AAGGGGTTTGTAATGAATGA+TGG 0.597722 2_2:+14355773 Msa0239770:three_prime_UTR
GCTCTCTTGTTGTCATAACC+AGG 0.598483 2_2:-14355485 None:intergenic
ATTTCTTTCTCAAGACTCAG+AGG 0.603232 2_2:+14353310 Msa0239770:CDS
TAAGCCAAAATAAAAGAAGT+GGG 0.605418 2_2:-14355621 None:intergenic
ATTCAAGGTTGAGCTGTGCA+TGG 0.618570 2_2:-14355920 None:intergenic
TAATGGAATGAGTAATGCAA+GGG 0.623573 2_2:+14353227 Msa0239770:CDS
TAACGATTGGTCAACAGGGT+CGG 0.624880 2_2:+14352784 Msa0239770:five_prime_UTR
GCACTAGTAAAGAAAGAGGG+AGG 0.626548 2_2:+14355344 Msa0239770:CDS
GATTCATGTAACTCACAAAG+AGG 0.650804 2_2:+14355370 Msa0239770:CDS
GGGTAAAGGAATCCAACATG+AGG 0.659464 2_2:-14353357 None:intergenic
TCTGGCAGTAACTATTCTCA+GGG 0.659879 2_2:-14353377 None:intergenic
AAGGCACTAGTAAAGAAAGA+GGG 0.660972 2_2:+14355341 Msa0239770:CDS
ATTCATGTAACTCACAAAGA+GGG 0.661176 2_2:+14355371 Msa0239770:CDS
AAGTACTGGCTTCCCCAACA+GGG 0.678090 2_2:-14355535 None:intergenic
AAAGTAACGATTGGTCAACA+GGG 0.679066 2_2:+14352780 Msa0239770:five_prime_UTR
TGTGTGGGAGAGAAATAGTG+AGG 0.705020 2_2:+14352708 Msa0239770:five_prime_UTR
TCAGTGTTAGTGTTAAGTTG+AGG 0.707444 2_2:-14352896 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAACAAAAATGAAAAA+CGG - chr2_2:14353160-14353179 None:intergenic 10.0%
!!! ATAATAACATCTTAAAATTT+TGG - chr2_2:14353554-14353573 None:intergenic 10.0%
!!! TAATAACATCTTAAAATTTT+GGG - chr2_2:14353553-14353572 None:intergenic 10.0%
!!! ATAATAACATCTTAAAATTT+TGG - chr2_2:14353554-14353573 None:intergenic 10.0%
!! AAGTTGAAAACTTTATTATA+CGG - chr2_2:14352748-14352767 None:intergenic 15.0%
!! AAAAATAGTAGTAAATGAAA+AGG - chr2_2:14353444-14353463 None:intergenic 15.0%
!!! AATAACATCTTAAAATTTTG+GGG - chr2_2:14353552-14353571 None:intergenic 15.0%
!! TTAAATAGAGATAAAACATA+TGG - chr2_2:14355686-14355705 None:intergenic 15.0%
!!! ATTGAAAAGAAGTATAGTAA+TGG + chr2_2:14353210-14353229 Msa0239770:CDS 20.0%
!! AAAAAGTAAAAAAGAGAGAA+AGG - chr2_2:14353416-14353435 None:intergenic 20.0%
!!! TAACATCTTAAAATTTTGGT+TGG - chr2_2:14353550-14353569 None:intergenic 20.0%
!!! ATAACATCTTAAAATTTTGG+GGG - chr2_2:14353551-14353570 None:intergenic 20.0%
!! CTATTTACCCAAAATTTATT+GGG + chr2_2:14354116-14354135 Msa0239770:intron 20.0%
!!! ATAAATCAGGGTTTTAAAAA+AGG - chr2_2:14354495-14354514 None:intergenic 20.0%
!!! AAGATAATAACATTTTATCG+AGG - chr2_2:14354524-14354543 None:intergenic 20.0%
!! TCGATAAAATGTTATTATCT+TGG + chr2_2:14354523-14354542 Msa0239770:intron 20.0%
!! AATAATCAGAATTTACATTG+CGG - chr2_2:14354597-14354616 None:intergenic 20.0%
!! TTTCTATTGCTATACTAATT+TGG - chr2_2:14354644-14354663 None:intergenic 20.0%
!! GTACTTCCTAATAAAATTTA+AGG + chr2_2:14354710-14354729 Msa0239770:intron 20.0%
!! TTCCTAATAAAATTTAAGGA+TGG + chr2_2:14354714-14354733 Msa0239770:intron 20.0%
!! TCCTAATAAAATTTAAGGAT+GGG + chr2_2:14354715-14354734 Msa0239770:intron 20.0%
!! AAAAAAGTAACAGCATAATT+TGG - chr2_2:14355710-14355729 None:intergenic 20.0%
!!! ACTTTTTTTTTTTCTTTCTG+TGG + chr2_2:14355722-14355741 Msa0239770:three_prime_UTR 20.0%
!!! TACTTAAACATTGTTGATAA+GGG + chr2_2:14355755-14355774 Msa0239770:three_prime_UTR 20.0%
! TTCTTTCATAAAGTAACGAT+TGG + chr2_2:14352771-14352790 Msa0239770:five_prime_UTR 25.0%
! ATTCAATGATGCATAGATTA+TGG - chr2_2:14352826-14352845 None:intergenic 25.0%
! AAACACAAAATTCTCTTTGT+TGG + chr2_2:14353007-14353026 Msa0239770:CDS 25.0%
! TCTTTATGATGTTGATGTTA+AGG + chr2_2:14353275-14353294 Msa0239770:CDS 25.0%
!! GTTTATAATTTTCCTCATGT+TGG + chr2_2:14353345-14353364 Msa0239770:CDS 25.0%
! CTACTTACAAATGTAACTTT+AGG - chr2_2:14353480-14353499 None:intergenic 25.0%
! CTAAAGTTACATTTGTAAGT+AGG + chr2_2:14353478-14353497 Msa0239770:intron 25.0%
!!! TCTTATAAGCCAGTTTTTTA+AGG + chr2_2:14353513-14353532 Msa0239770:intron 25.0%
! ATAGCCTATCCAAAATTTAT+TGG + chr2_2:14353572-14353591 Msa0239770:intron 25.0%
! TAGCCTATCCAAAATTTATT+GGG + chr2_2:14353573-14353592 Msa0239770:intron 25.0%
! ATAGCCTATCCAAAATTTAT+TGG + chr2_2:14353572-14353591 Msa0239770:intron 25.0%
!! TTGTCCAATAAATTTTGGAT+AGG - chr2_2:14353738-14353757 None:intergenic 25.0%
! TTAACGTTTCAATCTAATGT+GGG - chr2_2:14353867-14353886 None:intergenic 25.0%
! TAAACATACAAGGACTAAAA+TGG + chr2_2:14354093-14354112 Msa0239770:intron 25.0%
! GCTATTTACCCAAAATTTAT+TGG + chr2_2:14354115-14354134 Msa0239770:intron 25.0%
!! ACAATCTATATGTACAGTTT+TGG + chr2_2:14354156-14354175 Msa0239770:intron 25.0%
!! CAATCTATATGTACAGTTTT+GGG + chr2_2:14354157-14354176 Msa0239770:intron 25.0%
! CAACCTTGATCTTAAAATTT+GGG - chr2_2:14354201-14354220 None:intergenic 25.0%
! ACAACCTTGATCTTAAAATT+TGG - chr2_2:14354202-14354221 None:intergenic 25.0%
! CAAAAATCTGTGTAATATCA+TGG - chr2_2:14354252-14354271 None:intergenic 25.0%
!! CATGATATTACACAGATTTT+TGG + chr2_2:14354250-14354269 Msa0239770:intron 25.0%
! AAGGTCACATATACTTAAAT+CGG - chr2_2:14354373-14354392 None:intergenic 25.0%
! ACTATCTATGTTTAACATCA+AGG - chr2_2:14354463-14354482 None:intergenic 25.0%
! TTAAACATAGATAGTAATCG+CGG + chr2_2:14354468-14354487 Msa0239770:intron 25.0%
! TCGAGGATATATATAAATCA+GGG - chr2_2:14354507-14354526 None:intergenic 25.0%
! ATCGAGGATATATATAAATC+AGG - chr2_2:14354508-14354527 None:intergenic 25.0%
! AAATGTTATTATCTTGGACT+TGG + chr2_2:14354529-14354548 Msa0239770:intron 25.0%
! ATAATCAGAATTTACATTGC+GGG - chr2_2:14354596-14354615 None:intergenic 25.0%
!!! ATACTAATTTGGCTTGATTT+AGG - chr2_2:14354633-14354652 None:intergenic 25.0%
! GTATAGCAATAGAAATAAGT+AGG + chr2_2:14354649-14354668 Msa0239770:intron 25.0%
!! TCCCATCCTTAAATTTTATT+AGG - chr2_2:14354719-14354738 None:intergenic 25.0%
!! AGGTTTGACTAAATTTTACT+CGG + chr2_2:14354745-14354764 Msa0239770:intron 25.0%
! GTATGAAGAAACAAAAATAG+AGG - chr2_2:14354809-14354828 None:intergenic 25.0%
! CAAATATTGTCAAATAGTAG+CGG - chr2_2:14355054-14355073 None:intergenic 25.0%
! TTGAACAAAAAGTTGTTGAA+GGG + chr2_2:14355302-14355321 Msa0239770:CDS 25.0%
!!! AGATAGGCTATAATTTGTTA+GGG - chr2_2:14355586-14355605 None:intergenic 25.0%
!!! AAGATAGGCTATAATTTGTT+AGG - chr2_2:14355587-14355606 None:intergenic 25.0%
! TAAGCCAAAATAAAAGAAGT+GGG - chr2_2:14355624-14355643 None:intergenic 25.0%
! ATAAGCCAAAATAAAAGAAG+TGG - chr2_2:14355625-14355644 None:intergenic 25.0%
! ATGTTTAGATCTTCACTTTA+TGG + chr2_2:14355655-14355674 Msa0239770:three_prime_UTR 25.0%
! CTACTTAAACATTGTTGATA+AGG + chr2_2:14355754-14355773 Msa0239770:three_prime_UTR 25.0%
!! ACTTAAACATTGTTGATAAG+GGG + chr2_2:14355756-14355775 Msa0239770:three_prime_UTR 25.0%
! CACTATTCAAGATATTTCTT+TGG - chr2_2:14355897-14355916 None:intergenic 25.0%
! CAAAGAAATATCTTGAATAG+TGG + chr2_2:14355895-14355914 Msa0239770:three_prime_UTR 25.0%
!!! TGATCATGTTGTTTTTGTGT+TGG - chr2_2:14352875-14352894 None:intergenic 30.0%
AAAATTCTCTTTGTTGGAGA+GGG + chr2_2:14353013-14353032 Msa0239770:CDS 30.0%
! TTTCTCTCTTTGTTTAGCTA+GGG + chr2_2:14353045-14353064 Msa0239770:CDS 30.0%
AAAAACGGACATGCTAAAAA+AGG - chr2_2:14353145-14353164 None:intergenic 30.0%
TAATGGAATGAGTAATGCAA+GGG + chr2_2:14353227-14353246 Msa0239770:CDS 30.0%
AAAGGAAACATTACTGAATC+TGG - chr2_2:14353398-14353417 None:intergenic 30.0%
CTAAGTCAACCTTAAAAAAC+TGG - chr2_2:14353525-14353544 None:intergenic 30.0%
!!! CAGTTTTTTAAGGTTGACTT+AGG + chr2_2:14353523-14353542 Msa0239770:intron 30.0%
! TTGCCCAATAAATTTTGGAT+AGG - chr2_2:14353579-14353598 None:intergenic 30.0%
! GCTCTTTGTCCAATAAATTT+TGG - chr2_2:14353743-14353762 None:intergenic 30.0%
TAACGTTTCAATCTAATGTG+GGG - chr2_2:14353866-14353885 None:intergenic 30.0%
GTTAACGTTTCAATCTAATG+TGG - chr2_2:14353868-14353887 None:intergenic 30.0%
GTTAACATCTCAATCTTGTT+GGG + chr2_2:14354001-14354020 Msa0239770:intron 30.0%
TTAACATCTCAATCTTGTTG+GGG + chr2_2:14354002-14354021 Msa0239770:intron 30.0%
!!! CAACCCAAATTTTAAGATCA+AGG + chr2_2:14354195-14354214 Msa0239770:intron 30.0%
AGGTCACATATACTTAAATC+GGG - chr2_2:14354372-14354391 None:intergenic 30.0%
AGCAATAGAAATAAGTAGGT+AGG + chr2_2:14354653-14354672 Msa0239770:intron 30.0%
ATTTAAGGATGGGAATAGTA+AGG + chr2_2:14354725-14354744 Msa0239770:intron 30.0%
! GGTTTGACTAAATTTTACTC+GGG + chr2_2:14354746-14354765 Msa0239770:intron 30.0%
AAAGTTGTACAACAACTTCA+TGG - chr2_2:14354905-14354924 None:intergenic 30.0%
CGCTACTATTTGACAATATT+TGG + chr2_2:14355052-14355071 Msa0239770:intron 30.0%
GGTACATAAATACTGTATAG+CGG + chr2_2:14355073-14355092 Msa0239770:intron 30.0%
GTTGAACAAAAAGTTGTTGA+AGG + chr2_2:14355301-14355320 Msa0239770:CDS 30.0%
TGAACAAAAAGTTGTTGAAG+GGG + chr2_2:14355303-14355322 Msa0239770:CDS 30.0%
ATTCATGTAACTCACAAAGA+GGG + chr2_2:14355371-14355390 Msa0239770:CDS 30.0%
GTAAGAAAAGCTGAAAAAAG+AGG + chr2_2:14355419-14355438 Msa0239770:CDS 30.0%
!! TAGTCCCACTTCTTTTATTT+TGG + chr2_2:14355617-14355636 Msa0239770:three_prime_UTR 30.0%
ATCATCATTGCAGATGAATA+TGG - chr2_2:14355871-14355890 None:intergenic 30.0%
! TAAAGTAACGATTGGTCAAC+AGG + chr2_2:14352779-14352798 Msa0239770:five_prime_UTR 35.0%
! AAAGTAACGATTGGTCAACA+GGG + chr2_2:14352780-14352799 Msa0239770:five_prime_UTR 35.0%
! TCAGTGTTAGTGTTAAGTTG+AGG - chr2_2:14352899-14352918 None:intergenic 35.0%
CATCATTGAACACAGCAAAA+TGG + chr2_2:14352966-14352985 Msa0239770:CDS 35.0%
CAAAATTCTCTTTGTTGGAG+AGG + chr2_2:14353012-14353031 Msa0239770:CDS 35.0%
! CTTTCTCTCTTTGTTTAGCT+AGG + chr2_2:14353044-14353063 Msa0239770:CDS 35.0%
! GTAATGGAATGAGTAATGCA+AGG + chr2_2:14353226-14353245 Msa0239770:CDS 35.0%
ATTTCTTTCTCAAGACTCAG+AGG + chr2_2:14353310-14353329 Msa0239770:CDS 35.0%
AGTAACTATTCTCAGGGTAA+AGG - chr2_2:14353374-14353393 None:intergenic 35.0%
! GCTCTTTGCCCAATAAATTT+TGG - chr2_2:14353584-14353603 None:intergenic 35.0%
! TATTGGGCAAAGAGCAATTT+TGG + chr2_2:14353589-14353608 Msa0239770:intron 35.0%
! TATTGGACAAAGAGCAGTTT+TGG + chr2_2:14353748-14353767 Msa0239770:intron 35.0%
! GTAGGGGATTATTGTGTTTT+TGG - chr2_2:14353817-14353836 None:intergenic 35.0%
AACGTTTCAATCTAATGTGG+GGG - chr2_2:14353865-14353884 None:intergenic 35.0%
AGCTTATGTGACATATTGAG+AGG + chr2_2:14353900-14353919 Msa0239770:intron 35.0%
GGTTAACATCTCAATCTTGT+TGG + chr2_2:14354000-14354019 Msa0239770:intron 35.0%
! GACTATGACATCAAGGTTTT+TGG - chr2_2:14354318-14354337 None:intergenic 35.0%
AGTATATGTGACCTTAATCG+TGG + chr2_2:14354378-14354397 Msa0239770:intron 35.0%
GTAGTTGAATGGCACAAAAA+TGG - chr2_2:14354564-14354583 None:intergenic 35.0%
GGAAATGCTTAGTAGTTGAA+TGG - chr2_2:14354575-14354594 None:intergenic 35.0%
TGGCTTGATTTAGGAAATAG+AGG - chr2_2:14354624-14354643 None:intergenic 35.0%
TTATTGAAAGGAAACCACGA+GGG - chr2_2:14354855-14354874 None:intergenic 35.0%
TCGTGGTTTCCTTTCAATAA+GGG + chr2_2:14354855-14354874 Msa0239770:intron 35.0%
TCAAATAGTAGCGGCTATAA+TGG - chr2_2:14355045-14355064 None:intergenic 35.0%
CTGATCCATGTATTCTTGTT+TGG + chr2_2:14355159-14355178 Msa0239770:intron 35.0%
ATGCACCAAACAAGAATACA+TGG - chr2_2:14355167-14355186 None:intergenic 35.0%
!! TATTCTTGTTTGGTGCATTG+TGG + chr2_2:14355169-14355188 Msa0239770:intron 35.0%
GGAGGAGAAATTACTTTGAA+AGG - chr2_2:14355225-14355244 None:intergenic 35.0%
! AACTTTTTGTTCAACCTGCT+TGG - chr2_2:14355296-14355315 None:intergenic 35.0%
AAAGTTGTTGAAGGGGTTTA+TGG + chr2_2:14355310-14355329 Msa0239770:CDS 35.0%
!! AAAGGCACTAGTAAAGAAAG+AGG + chr2_2:14355340-14355359 Msa0239770:CDS 35.0%
! AAGGCACTAGTAAAGAAAGA+GGG + chr2_2:14355341-14355360 Msa0239770:CDS 35.0%
GATTCATGTAACTCACAAAG+AGG + chr2_2:14355370-14355389 Msa0239770:CDS 35.0%
!! ACAAGATCCCATTTGTTGTA+TGG - chr2_2:14355401-14355420 None:intergenic 35.0%
! GGATAGTCATCTTTGAAGAA+TGG - chr2_2:14355467-14355486 None:intergenic 35.0%
TTCTTCAAAGATGACTATCC+TGG + chr2_2:14355467-14355486 Msa0239770:CDS 35.0%
TCAGATTTATCTTTCCCTGT+TGG + chr2_2:14355521-14355540 Msa0239770:CDS 35.0%
CAGATTTATCTTTCCCTGTT+GGG + chr2_2:14355522-14355541 Msa0239770:CDS 35.0%
AGATTTATCTTTCCCTGTTG+GGG + chr2_2:14355523-14355542 Msa0239770:CDS 35.0%
AAGGGGTTTGTAATGAATGA+TGG + chr2_2:14355773-14355792 Msa0239770:three_prime_UTR 35.0%
TTAGTCCAAAGTCTAAACTC+TGG + chr2_2:14355839-14355858 Msa0239770:three_prime_UTR 35.0%
CATTTCCAGAGTTTAGACTT+TGG - chr2_2:14355847-14355866 None:intergenic 35.0%
ATTACTATTACGTGCGTGTG+TGG + chr2_2:14352692-14352711 Msa0239770:five_prime_UTR 40.0%
TTACTATTACGTGCGTGTGT+GGG + chr2_2:14352693-14352712 Msa0239770:five_prime_UTR 40.0%
!! CTTTGTTTAGCTAGGGCTTT+TGG + chr2_2:14353052-14353071 Msa0239770:CDS 40.0%
AATCAAGGGAAGTGGCTATA+AGG - chr2_2:14353090-14353109 None:intergenic 40.0%
TGCATTACCCTGAGAATCAA+GGG - chr2_2:14353104-14353123 None:intergenic 40.0%
TTGCATTACCCTGAGAATCA+AGG - chr2_2:14353105-14353124 None:intergenic 40.0%
TCTGGCAGTAACTATTCTCA+GGG - chr2_2:14353380-14353399 None:intergenic 40.0%
ATCTGGCAGTAACTATTCTC+AGG - chr2_2:14353381-14353400 None:intergenic 40.0%
!! CAAAGAGCAATTTTGGTCCT+TGG + chr2_2:14353596-14353615 Msa0239770:intron 40.0%
TAACAGACTGCAAAGTGAGT+GGG - chr2_2:14353676-14353695 None:intergenic 40.0%
! TTTGCTTTCATTTTCGTCCC+TGG - chr2_2:14353948-14353967 None:intergenic 40.0%
GACAGTGAGGTAAACATACA+AGG + chr2_2:14354083-14354102 Msa0239770:intron 40.0%
! TGGGTGGCCCAATAAATTTT+GGG - chr2_2:14354126-14354145 None:intergenic 40.0%
ATAGATTGTGACCTGAGAGT+GGG - chr2_2:14354145-14354164 None:intergenic 40.0%
TATAGATTGTGACCTGAGAG+TGG - chr2_2:14354146-14354165 None:intergenic 40.0%
TGAGATTAACGTCGCATCAA+CGG - chr2_2:14354283-14354302 None:intergenic 40.0%
AAACCTTGATGTCATAGTCC+AGG + chr2_2:14354319-14354338 Msa0239770:intron 40.0%
AAATGTCTATGACCACAACC+TGG - chr2_2:14354340-14354359 None:intergenic 40.0%
ACACAATGTGACCTTAATCG+TGG + chr2_2:14354414-14354433 Msa0239770:intron 40.0%
CTTATTGAAAGGAAACCACG+AGG - chr2_2:14354856-14354875 None:intergenic 40.0%
CTCGTGGTTTCCTTTCAATA+AGG + chr2_2:14354854-14354873 Msa0239770:intron 40.0%
CGTGGTTTCCTTTCAATAAG+GGG + chr2_2:14354856-14354875 Msa0239770:intron 40.0%
TCCTTTCAATAAGGGGTTCT+TGG + chr2_2:14354863-14354882 Msa0239770:intron 40.0%
TCCAAGAACCCCTTATTGAA+AGG - chr2_2:14354867-14354886 None:intergenic 40.0%
! TCCATCAGTGTCGTTAATTG+TGG + chr2_2:14354966-14354985 Msa0239770:intron 40.0%
! CCATCAGTGTCGTTAATTGT+GGG + chr2_2:14354967-14354986 Msa0239770:intron 40.0%
TTGTGGGCAGCAGAAAATAT+CGG + chr2_2:14354983-14355002 Msa0239770:intron 40.0%
TACACTGTCTCAATAGATGG+AGG - chr2_2:14355243-14355262 None:intergenic 40.0%
CCATACACTGTCTCAATAGA+TGG - chr2_2:14355246-14355265 None:intergenic 40.0%
CCATCTATTGAGACAGTGTA+TGG + chr2_2:14355243-14355262 Msa0239770:intron 40.0%
TGAATTTGTGACAACCAAGC+AGG + chr2_2:14355279-14355298 Msa0239770:intron 40.0%
CACTAGTAAAGAAAGAGGGA+GGG + chr2_2:14355345-14355364 Msa0239770:CDS 40.0%
ACTAGTAAAGAAAGAGGGAG+GGG + chr2_2:14355346-14355365 Msa0239770:CDS 40.0%
AGGGTGATCCATACAACAAA+TGG + chr2_2:14355390-14355409 Msa0239770:CDS 40.0%
GGGTGATCCATACAACAAAT+GGG + chr2_2:14355391-14355410 Msa0239770:CDS 40.0%
! CTTTGAAGAATGGAACAGCT+TGG - chr2_2:14355457-14355476 None:intergenic 40.0%
TATGACAACAAGAGAGCTCA+TGG + chr2_2:14355491-14355510 Msa0239770:CDS 40.0%
GAGCTTGAACTTGTAAGTAC+TGG - chr2_2:14355552-14355571 None:intergenic 40.0%
!! ATTTGTTAGGGAGAGGTGTT+TGG - chr2_2:14355574-14355593 None:intergenic 40.0%
!! GGCTATAATTTGTTAGGGAG+AGG - chr2_2:14355581-14355600 None:intergenic 40.0%
GACTAGAAGTTGGCAAAGAT+AGG - chr2_2:14355602-14355621 None:intergenic 40.0%
AAAGAAGTGGGACTAGAAGT+TGG - chr2_2:14355612-14355631 None:intergenic 40.0%
TGTGTGGGAGAGAAATAGTG+AGG + chr2_2:14352708-14352727 Msa0239770:five_prime_UTR 45.0%
! TAACGATTGGTCAACAGGGT+CGG + chr2_2:14352784-14352803 Msa0239770:five_prime_UTR 45.0%
GCAAGGGAACTTGAAGAAAG+AGG + chr2_2:14353243-14353262 Msa0239770:CDS 45.0%
GGGTAAAGGAATCCAACATG+AGG - chr2_2:14353360-14353379 None:intergenic 45.0%
TGTCATAGTAGTCCCTGACT+CGG + chr2_2:14353632-14353651 Msa0239770:intron 45.0%
!!! TTGTGTTTTTGGCCGAGTCA+GGG - chr2_2:14353647-14353666 None:intergenic 45.0%
!!! ATTGTGTTTTTGGCCGAGTC+AGG - chr2_2:14353648-14353667 None:intergenic 45.0%
! GTGGGGGGTTATTGTGTTTT+TGG - chr2_2:14353658-14353677 None:intergenic 45.0%
AACAGACTGCAAAGTGAGTG+GGG - chr2_2:14353675-14353694 None:intergenic 45.0%
CTAACAGACTGCAAAGTGAG+TGG - chr2_2:14353677-14353696 None:intergenic 45.0%
!! CAAAGAGCAGTTTTGGTCCT+TGG + chr2_2:14353755-14353774 Msa0239770:intron 45.0%
TGTCATAGTAGTCCCTGACT+CGG + chr2_2:14353632-14353651 Msa0239770:intron 45.0%
!!! TTGTGTTTTTGGCCGAGTCA+GGG - chr2_2:14353647-14353666 None:intergenic 45.0%
!!! ATTGTGTTTTTGGCCGAGTC+AGG - chr2_2:14353648-14353667 None:intergenic 45.0%
TGACATATTGAGAGGCCAGT+AGG + chr2_2:14353908-14353927 Msa0239770:intron 45.0%
CAAATCCCACTTTGTCACGT+CGG + chr2_2:14353964-14353983 Msa0239770:intron 45.0%
ATGACCAACAGAATGCAGAG+TGG + chr2_2:14354031-14354050 Msa0239770:intron 45.0%
TGACCAACAGAATGCAGAGT+GGG + chr2_2:14354032-14354051 Msa0239770:intron 45.0%
TCAGGACTACTATGACAGTG+AGG + chr2_2:14354070-14354089 Msa0239770:intron 45.0%
! GTGGGTGGCCCAATAAATTT+TGG - chr2_2:14354127-14354146 None:intergenic 45.0%
CAACCTGGACTATGACATCA+AGG - chr2_2:14354325-14354344 None:intergenic 45.0%
TGATGTCATAGTCCAGGTTG+TGG + chr2_2:14354325-14354344 Msa0239770:intron 45.0%
TTGCAGCACAACCACGATTA+AGG - chr2_2:14354392-14354411 None:intergenic 45.0%
CAATAAGGGGTTCTTGGAAC+TGG + chr2_2:14354869-14354888 Msa0239770:intron 45.0%
CCCACAATTAACGACACTGA+TGG - chr2_2:14354970-14354989 None:intergenic 45.0%
!! GTTTGGTGCATTGTGGACTA+TGG + chr2_2:14355176-14355195 Msa0239770:intron 45.0%
GGGGTTTATGGCAAATGCAA+AGG + chr2_2:14355322-14355341 Msa0239770:CDS 45.0%
GCACTAGTAAAGAAAGAGGG+AGG + chr2_2:14355344-14355363 Msa0239770:CDS 45.0%
! GCTCTCTTGTTGTCATAACC+AGG - chr2_2:14355488-14355507 None:intergenic 45.0%
AGCCACTTCCCTTGATTCTC+AGG + chr2_2:14353093-14353112 Msa0239770:CDS 50.0%
GCCACTTCCCTTGATTCTCA+GGG + chr2_2:14353094-14353113 Msa0239770:CDS 50.0%
ACCCTGAGAATCAAGGGAAG+TGG - chr2_2:14353098-14353117 None:intergenic 50.0%
GACATCGAGGTACACATCCA+AGG - chr2_2:14353616-14353635 None:intergenic 50.0%
CAGGGACTACTATGACATCG+AGG - chr2_2:14353629-14353648 None:intergenic 50.0%
ACAGACTGCAAAGTGAGTGG+GGG - chr2_2:14353674-14353693 None:intergenic 50.0%
GACATCGAGGTACACATCCA+AGG - chr2_2:14353616-14353635 None:intergenic 50.0%
CAGGGACTACTATGACATCG+AGG - chr2_2:14353629-14353648 None:intergenic 50.0%
!! ACGAACGGACTGCAAAGTAG+GGG - chr2_2:14353833-14353852 None:intergenic 50.0%
!! GACGAACGGACTGCAAAGTA+GGG - chr2_2:14353834-14353853 None:intergenic 50.0%
!! TGACGAACGGACTGCAAAGT+AGG - chr2_2:14353835-14353854 None:intergenic 50.0%
TGGGGGACTACTATGACGAA+CGG - chr2_2:14353848-14353867 None:intergenic 50.0%
TATTGAGAGGCCAGTAGGCA+TGG + chr2_2:14353913-14353932 Msa0239770:intron 50.0%
GTACACTTCACCATGCCTAC+TGG - chr2_2:14353926-14353945 None:intergenic 50.0%
TAGGCATGGTGAAGTGTACC+AGG + chr2_2:14353927-14353946 Msa0239770:intron 50.0%
AGGCATGGTGAAGTGTACCA+GGG + chr2_2:14353928-14353947 Msa0239770:intron 50.0%
! CACGTCGGCTGACAAATCAT+CGG + chr2_2:14353979-14353998 Msa0239770:intron 50.0%
GACCAACAGAATGCAGAGTG+GGG + chr2_2:14354033-14354052 Msa0239770:intron 50.0%
ACCAACAGAATGCAGAGTGG+GGG + chr2_2:14354034-14354053 Msa0239770:intron 50.0%
AATGCAGAGTGGGGGGTTAT+TGG + chr2_2:14354042-14354061 Msa0239770:intron 50.0%
TTGCAGCGCAACCACGATTA+AGG - chr2_2:14354428-14354447 None:intergenic 50.0%
ATGAACCAAGTTAGCCCTCG+TGG + chr2_2:14354838-14354857 Msa0239770:intron 50.0%
!! AAGTACTGGCTTCCCCAACA+GGG - chr2_2:14355538-14355557 None:intergenic 50.0%
!! TAAGTACTGGCTTCCCCAAC+AGG - chr2_2:14355539-14355558 None:intergenic 50.0%
CAGACTGCAAAGTGAGTGGG+GGG - chr2_2:14353673-14353692 None:intergenic 55.0%
GTCAGCCGACGTGACAAAGT+GGG - chr2_2:14353972-14353991 None:intergenic 55.0%
TGTCAGCCGACGTGACAAAG+TGG - chr2_2:14353973-14353992 None:intergenic 55.0%
CCAACAGAATGCAGAGTGGG+GGG + chr2_2:14354035-14354054 Msa0239770:intron 55.0%
!! GGGGGGTTATTGGCTCAATC+AGG + chr2_2:14354052-14354071 Msa0239770:intron 55.0%
TTATTGGGCCACCCACTCTC+AGG + chr2_2:14354131-14354150 Msa0239770:intron 55.0%
GATTGTGACCTGAGAGTGGG+TGG - chr2_2:14354142-14354161 None:intergenic 55.0%
GGAAACCACGAGGGCTAACT+TGG - chr2_2:14354846-14354865 None:intergenic 55.0%
CCCCCCACTCTGCATTCTGT+TGG - chr2_2:14354038-14354057 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_2 gene 14352660 14355929 14352660 ID=Msa0239770;Name=Msa0239770
chr2_2 mRNA 14352660 14355929 14352660 ID=Msa0239770-mRNA-1;Parent=Msa0239770;Name=Msa0239770-mRNA-1;_AED=0.00;_eAED=0.00;_QI=206|1|1|1|1|1|3|331|248
chr2_2 exon 14352660 14353115 14352660 ID=Msa0239770-mRNA-1:exon:5927;Parent=Msa0239770-mRNA-1
chr2_2 exon 14353205 14353403 14353205 ID=Msa0239770-mRNA-1:exon:5928;Parent=Msa0239770-mRNA-1
chr2_2 exon 14355301 14355929 14355301 ID=Msa0239770-mRNA-1:exon:5929;Parent=Msa0239770-mRNA-1
chr2_2 five_prime_UTR 14352660 14352865 14352660 ID=Msa0239770-mRNA-1:five_prime_utr;Parent=Msa0239770-mRNA-1
chr2_2 CDS 14352866 14353115 14352866 ID=Msa0239770-mRNA-1:cds;Parent=Msa0239770-mRNA-1
chr2_2 CDS 14353205 14353403 14353205 ID=Msa0239770-mRNA-1:cds;Parent=Msa0239770-mRNA-1
chr2_2 CDS 14355301 14355598 14355301 ID=Msa0239770-mRNA-1:cds;Parent=Msa0239770-mRNA-1
chr2_2 three_prime_UTR 14355599 14355929 14355599 ID=Msa0239770-mRNA-1:three_prime_utr;Parent=Msa0239770-mRNA-1
Gene Sequence

>Msa0239770

ATGAGTCCAACACAAAAACAACATGATCATCCTCAACTTAACACTAACACTGACACTGATGATGAAGAACAAGAATCAGAATCAGAGTCAGAAGAAGAATCATCATTGAACACAGCAAAATGGAAAAAACACTACTCATCAAAACACAAAATTCTCTTTGTTGGAGAGGGTGATTTTTCTTTCTCTCTTTGTTTAGCTAGGGCTTTTGGTTCTGCTCATAACCTTATAGCCACTTCCCTTGATTCTCAGGAGAAAATTGAAAAGAAGTATAGTAATGGAATGAGTAATGCAAGGGAACTTGAAGAAAGAGGTTGTATTGTTCTTTATGATGTTGATGTTAAGGTGATGAGTCAGCATTTCTTTCTCAAGACTCAGAGGTTTGATCGTGTTGTTTATAATTTTCCTCATGTTGGATTCCTTTACCCTGAGAATAGTTACTGCCAGATTCAGTTGAACAAAAAGTTGTTGAAGGGGTTTATGGCAAATGCAAAGGCACTAGTAAAGAAAGAGGGAGGGGAGATTCATGTAACTCACAAAGAGGGTGATCCATACAACAAATGGGATCTTGTAAGAAAAGCTGAAAAAAGAGGACTGTTCTTACACCAAGCTGTTCCATTCTTCAAAGATGACTATCCTGGTTATGACAACAAGAGAGCTCATGGAAAGTTATCAGATTTATCTTTCCCTGTTGGGGAAGCCAGTACTTACAAGTTCAAGCTCCAAACACCTCTCCCTAACAAATTATAG
Protein sequence

>Msa0239770

MSPTQKQHDHPQLNTNTDTDDEEQESESESEEESSLNTAKWKKHYSSKHKILFVGEGDFSFSLCLARAFGSAHNLIATSLDSQEKIEKKYSNGMSNARELEERGCIVLYDVDVKVMSQHFFLKTQRFDRVVYNFPHVGFLYPENSYCQIQLNKKLLKGFMANAKALVKKEGGEIHVTHKEGDPYNKWDLVRKAEKRGLFLHQAVPFFKDDYPGYDNKRAHGKLSDLSFPVGEASTYKFKLQTPLPNKL*