AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0317480

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0317480 MtrunA17_Chr2g0290801 92.771 249 17 1 11 258 1 249 6.21e-169 466
Msa0317480 MtrunA17_Chr5g0444781 51.759 199 95 1 50 248 8 205 8.52e-70 217
Msa0317480 MtrunA17_Chr5g0444781 64.706 51 18 0 50 100 257 307 1.59e-13 69.3
Msa0317480 MtrunA17_Chr6g0483551 48.500 200 101 2 50 248 4 202 1.64e-61 192
Msa0317480 MtrunA17_Chr6g0483591 49.231 195 98 1 54 248 8 201 3.13e-61 192
Msa0317480 MtrunA17_Chr6g0483571 47.980 198 101 2 54 250 8 204 4.89e-60 189
Msa0317480 MtrunA17_Chr3g0103011 41.290 155 90 1 94 248 16 169 6.36e-34 126
Msa0317480 MtrunA17_Chr5g0444791 40.252 159 83 4 94 248 10 160 6.44e-29 109
Msa0317480 MtrunA17_Chr3g0103001 67.442 43 14 0 50 92 35 77 4.20e-15 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0317480 AT4G26485.1 48.529 204 101 3 48 248 4 206 6.94e-59 186
Msa0317480 AT5G56075.1 47.196 214 94 3 41 251 56 253 1.83e-56 181
Msa0317480 AT1G55790.1 43.662 213 119 1 38 250 7 218 1.23e-54 181
Msa0317480 AT5G25030.1 43.367 196 97 2 54 249 11 192 4.08e-48 158
Msa0317480 AT1G55803.1 49.593 123 62 0 38 160 5 127 1.71e-35 123
Msa0317480 AT5G56060.1 37.333 150 91 2 90 238 2 149 1.25e-30 112
Msa0317480 AT2G11045.1 33.121 157 69 4 94 249 14 135 1.79e-16 74.3
Msa0317480 AT1G55800.1 39.773 88 52 1 163 250 10 96 4.81e-14 70.1

Find 72 sgRNAs with CRISPR-Local

Find 193 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTTTAGATCTTCACTTTA+TGG 0.097788 2_4:+15374616 Msa0317480:three_prime_UTR
TAAAGTATTGATTGGTCATT+AGG 0.202504 2_4:+15372072 Msa0317480:five_prime_UTR
CTTTGTTTAGCTAGGGCTTT+TGG 0.226805 2_4:+15372345 Msa0317480:CDS
AAAGTTGTTGAAGGGGTTTA+TGG 0.239937 2_4:+15374271 Msa0317480:CDS
AAGATAGGCTATAATTTGTT+AGG 0.280357 2_4:-15374545 None:intergenic
TACTTAAACATTGTTGATAA+GGG 0.295882 2_4:+15374714 Msa0317480:three_prime_UTR
AAACACAAAATTCTCTTTGT+TGG 0.296100 2_4:+15372300 Msa0317480:CDS
ATTCAATCATGCATAGATTA+TGG 0.315340 2_4:-15372116 None:intergenic
AAGTTGAAAACTTTATTATA+CGG 0.322819 2_4:-15372038 None:intergenic
AGCCACTTCCCTTGATTCTC+AGG 0.330415 2_4:+15372386 Msa0317480:CDS
CTTTCTCTCTTTGTTTAGCT+AGG 0.331146 2_4:+15372337 Msa0317480:CDS
CAGATTTATCTTTCCCTGTT+GGG 0.342684 2_4:+15374483 Msa0317480:CDS
AAAGGAAACATTACTGAATC+TGG 0.348551 2_4:-15372696 None:intergenic
ACAAGATCCCATTTGTTGTA+TGG 0.352391 2_4:-15374359 None:intergenic
CTACTTAAACATTGTTGATA+AGG 0.352461 2_4:+15374713 Msa0317480:three_prime_UTR
CATCATCGAACACAGCAAAA+TGG 0.353869 2_4:+15372259 Msa0317480:CDS
AATCAAGGGAAGTGGCTATA+AGG 0.366752 2_4:-15372380 None:intergenic
TAAGTACTGGCTTCCCCAAC+AGG 0.374095 2_4:-15374497 None:intergenic
ATTTGTTAGGGAGAGGTGTT+TGG 0.378150 2_4:-15374532 None:intergenic
AGGGTGATCCATACAACAAA+TGG 0.390906 2_4:+15374351 Msa0317480:CDS
TTCTTTCATAAAGTATTGAT+TGG 0.393182 2_4:+15372064 Msa0317480:five_prime_UTR
AAAGTATTGATTGGTCATTA+GGG 0.396583 2_4:+15372073 Msa0317480:five_prime_UTR
TCTTTATGATGTTGATGTTA+AGG 0.404489 2_4:+15372576 Msa0317480:CDS
CAAAATTCTCTTTGTTGGAG+AGG 0.419240 2_4:+15372305 Msa0317480:CDS
ATCATCATTGCAGATGAATA+TGG 0.425955 2_4:-15374827 None:intergenic
TCAGATTTATCTTTCCCTGT+TGG 0.434452 2_4:+15374482 Msa0317480:CDS
ATCTGGCAGTAACTATTCTC+AGG 0.435024 2_4:-15372679 None:intergenic
TTTCTCTCTTTGTTTAGCTA+GGG 0.444044 2_4:+15372338 Msa0317480:CDS
TTAAATAGAGATAAAACATA+TGG 0.452221 2_4:-15374644 None:intergenic
TTGCATTACCCTGAGAATCA+AGG 0.455310 2_4:-15372395 None:intergenic
GAGCTTGAACTTGTAAGTAC+TGG 0.474156 2_4:-15374510 None:intergenic
GGCTATAATTTGTTAGGGAG+AGG 0.484720 2_4:-15374539 None:intergenic
CATTTCCAGAGTTTAGACTT+TGG 0.490874 2_4:-15374803 None:intergenic
GGATAGTCATCTTTGAAGAA+TGG 0.508198 2_4:-15374425 None:intergenic
TATGACAACAAGAGAGCTCA+TGG 0.509129 2_4:+15374452 Msa0317480:CDS
AGATAGGCTATAATTTGTTA+GGG 0.513605 2_4:-15374544 None:intergenic
TTCTTCAAAGATGACTATCC+TGG 0.517775 2_4:+15374428 Msa0317480:CDS
AAAGAAGTGGGACTAGAAGT+TGG 0.519198 2_4:-15374570 None:intergenic
AAAATTCTCTTTGTTGGAGA+GGG 0.521483 2_4:+15372306 Msa0317480:CDS
AGATTTATCTTTCCCTGTTG+GGG 0.524030 2_4:+15374484 Msa0317480:CDS
ACTAGTAAAGAAAGAGGGAG+GGG 0.524780 2_4:+15374307 Msa0317480:CDS
AGTAACTATTCTCAGGGTAA+AGG 0.525453 2_4:-15372672 None:intergenic
ATTGAAAAGAAGTATAGTAA+TGG 0.531936 2_4:+15372511 Msa0317480:CDS
AAAGGCACTAGTAAAGAAAG+AGG 0.540011 2_4:+15374301 Msa0317480:CDS
TGCATTACCCTGAGAATCAA+GGG 0.540315 2_4:-15372394 None:intergenic
GGGTGATCCATACAACAAAT+GGG 0.540448 2_4:+15374352 Msa0317480:CDS
GCCACTTCCCTTGATTCTCA+GGG 0.542014 2_4:+15372387 Msa0317480:CDS
TTAGTCCAAAGTCTAAACTC+TGG 0.561802 2_4:+15374798 Msa0317480:three_prime_UTR
GCAAGGGAACTTGAAGAAAG+AGG 0.562096 2_4:+15372544 Msa0317480:CDS
ACTTAAACATTGTTGATAAG+GGG 0.562256 2_4:+15374715 Msa0317480:three_prime_UTR
ACCCTGAGAATCAAGGGAAG+TGG 0.567810 2_4:-15372388 None:intergenic
ATAAGCCAAAATAAAAGAAG+TGG 0.568651 2_4:-15374583 None:intergenic
CTTTGAAGAATGGAACAGCT+TGG 0.571652 2_4:-15374415 None:intergenic
GTAATGGAATGAGTAATGCA+AGG 0.572801 2_4:+15372527 Msa0317480:CDS
GACTAGAAGTTGGCAAAGAT+AGG 0.577531 2_4:-15374560 None:intergenic
TATTGATTGGTCATTAGGGT+CGG 0.582558 2_4:+15372077 Msa0317480:five_prime_UTR
CACTAGTAAAGAAAGAGGGA+GGG 0.592759 2_4:+15374306 Msa0317480:CDS
GGGGTTTATGGCAAATGCAA+AGG 0.596492 2_4:+15374283 Msa0317480:CDS
AAGGGGTTTGTAATGAATGA+TGG 0.597722 2_4:+15374732 Msa0317480:three_prime_UTR
GCTCTCTTGTTGTCATAACC+AGG 0.598483 2_4:-15374446 None:intergenic
ATTTCTTTCTCAAGACTCAG+AGG 0.603232 2_4:+15372611 Msa0317480:CDS
TAAGCCAAAATAAAAGAAGT+GGG 0.605418 2_4:-15374582 None:intergenic
ATTAAAGGTTGAGCTGTGCA+TGG 0.616274 2_4:-15374879 None:intergenic
TAATGGAATGAGTAATGCAA+GGG 0.623573 2_4:+15372528 Msa0317480:CDS
GCACTAGTAAAGAAAGAGGG+AGG 0.626548 2_4:+15374305 Msa0317480:CDS
GATTCATGTAACTCACAAAG+AGG 0.650804 2_4:+15374331 Msa0317480:CDS
GGGTAAAGGAATCCAACATG+AGG 0.659464 2_4:-15372658 None:intergenic
TCTGGCAGTAACTATTCTCA+GGG 0.659879 2_4:-15372678 None:intergenic
AAGGCACTAGTAAAGAAAGA+GGG 0.660972 2_4:+15374302 Msa0317480:CDS
ATTCATGTAACTCACAAAGA+GGG 0.661176 2_4:+15374332 Msa0317480:CDS
AAGTACTGGCTTCCCCAACA+GGG 0.678090 2_4:-15374496 None:intergenic
TCAGTGACAGTGTTAAGTTG+AGG 0.766236 2_4:-15372189 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAACAAAAATGAAAAA+TGG - chr2_4:15372453-15372472 None:intergenic 10.0%
!!! ATAATAACATCTTAAAATTT+TGG - chr2_4:15372855-15372874 None:intergenic 10.0%
!! AAGTTGAAAACTTTATTATA+CGG - chr2_4:15372041-15372060 None:intergenic 15.0%
!! AAAAAGTAAAAAAGAAAGAA+AGG - chr2_4:15372717-15372736 None:intergenic 15.0%
!!! AAGATAATAACATTTTATCT+AGG - chr2_4:15373524-15373543 None:intergenic 15.0%
!! TAGATAAAATGTTATTATCT+TGG + chr2_4:15373523-15373542 Msa0317480:intron 15.0%
!! TTAAATAGAGATAAAACATA+TGG - chr2_4:15374647-15374666 None:intergenic 15.0%
!!! TTCTTTCATAAAGTATTGAT+TGG + chr2_4:15372064-15372083 Msa0317480:five_prime_UTR 20.0%
!!! ATTGAAAAGAAGTATAGTAA+TGG + chr2_4:15372511-15372530 Msa0317480:CDS 20.0%
!!! TAACATCTTAAAATTTTGGT+TGG - chr2_4:15372851-15372870 None:intergenic 20.0%
!! TTAACATCTCAATTTAATGA+TGG + chr2_4:15373077-15373096 Msa0317480:intron 20.0%
!! TAACATCTCAATTTAATGAT+GGG + chr2_4:15373078-15373097 Msa0317480:intron 20.0%
!! ATTTAAGGATGGAAATATTA+AGG + chr2_4:15373725-15373744 Msa0317480:intron 20.0%
!!! CGGGATAATAATATATTTTA+TGG + chr2_4:15373765-15373784 Msa0317480:intron 20.0%
!! AAAAAAGTAACAGCATAATT+TGG - chr2_4:15374671-15374690 None:intergenic 20.0%
!!! TTACTTTTTTTTTCTTTCTG+TGG + chr2_4:15374681-15374700 Msa0317480:three_prime_UTR 20.0%
!!! TACTTAAACATTGTTGATAA+GGG + chr2_4:15374714-15374733 Msa0317480:three_prime_UTR 20.0%
!!! CACTATTCAAGATATTTTTT+TGG - chr2_4:15374856-15374875 None:intergenic 20.0%
!! CAAAAAAATATCTTGAATAG+TGG + chr2_4:15374854-15374873 Msa0317480:three_prime_UTR 20.0%
!!! TAAAGTATTGATTGGTCATT+AGG + chr2_4:15372072-15372091 Msa0317480:five_prime_UTR 25.0%
!!! AAAGTATTGATTGGTCATTA+GGG + chr2_4:15372073-15372092 Msa0317480:five_prime_UTR 25.0%
! ATTCAATCATGCATAGATTA+TGG - chr2_4:15372119-15372138 None:intergenic 25.0%
! AAACACAAAATTCTCTTTGT+TGG + chr2_4:15372300-15372319 Msa0317480:CDS 25.0%
! AAAAATGGACATGCTAAAAA+AGG - chr2_4:15372438-15372457 None:intergenic 25.0%
! TCTTTATGATGTTGATGTTA+AGG + chr2_4:15372576-15372595 Msa0317480:CDS 25.0%
!! GTTTATAATTTTCCTCATGT+TGG + chr2_4:15372646-15372665 Msa0317480:CDS 25.0%
! CTACTTACAAATGTAACTTT+AGG - chr2_4:15372781-15372800 None:intergenic 25.0%
! CTAAAGTTACATTTGTAAGT+AGG + chr2_4:15372779-15372798 Msa0317480:intron 25.0%
! CCTTAAAAAACTGGATTATA+AGG - chr2_4:15372817-15372836 None:intergenic 25.0%
!!! CCTTATAATCCAGTTTTTTA+AGG + chr2_4:15372814-15372833 Msa0317480:intron 25.0%
! TTAAGTCAACCTTAAAAAAC+TGG - chr2_4:15372826-15372845 None:intergenic 25.0%
! ATAGCCTATCCAAAATTTAT+TGG + chr2_4:15372873-15372892 Msa0317480:intron 25.0%
! TAGCCTATCCAAAATTTATT+GGG + chr2_4:15372874-15372893 Msa0317480:intron 25.0%
!! TTGATGTCTCAATTTAACTT+GGG - chr2_4:15372992-15373011 None:intergenic 25.0%
! ATTGATGTCTCAATTTAACT+TGG - chr2_4:15372993-15373012 None:intergenic 25.0%
! AACATCTCAATTTAATGATG+GGG + chr2_4:15373079-15373098 Msa0317480:intron 25.0%
!!! TTACCCAAATTTTAAGATCA+AGG + chr2_4:15373227-15373246 Msa0317480:intron 25.0%
! CAACCTTGATCTTAAAATTT+GGG - chr2_4:15373233-15373252 None:intergenic 25.0%
! ACAACCTTGATCTTAAAATT+TGG - chr2_4:15373234-15373253 None:intergenic 25.0%
! AGGTCAAATATACTTAAATC+AGG - chr2_4:15373404-15373423 None:intergenic 25.0%
!!! ATAAACCAGGGTTTTAAAAA+AGG - chr2_4:15373495-15373514 None:intergenic 25.0%
!! TCTAGGATATATATAAACCA+GGG - chr2_4:15373507-15373526 None:intergenic 25.0%
!! ATCTAGGATATATATAAACC+AGG - chr2_4:15373508-15373527 None:intergenic 25.0%
! AAATGTTATTATCTTGGACT+TGG + chr2_4:15373529-15373548 Msa0317480:intron 25.0%
! AATAGTCAGAATTTACATTG+GGG - chr2_4:15373596-15373615 None:intergenic 25.0%
! CAATAGTCAGAATTTACATT+GGG - chr2_4:15373597-15373616 None:intergenic 25.0%
! TCAATAGTCAGAATTTACAT+TGG - chr2_4:15373598-15373617 None:intergenic 25.0%
! TTTCTATTGCTACACTAATT+TGG - chr2_4:15373643-15373662 None:intergenic 25.0%
! TGTAGCAATAGAAATTAAGT+AGG + chr2_4:15373649-15373668 Msa0317480:intron 25.0%
! TTCCTAATAGAATTTAAGGA+TGG + chr2_4:15373714-15373733 Msa0317480:intron 25.0%
! TTCCATCCTTAAATTCTATT+AGG - chr2_4:15373719-15373738 None:intergenic 25.0%
!! AGGTTTGACTAAATTTTACT+CGG + chr2_4:15373745-15373764 Msa0317480:intron 25.0%
! GTATGAAGAAACAAAAATAG+AGG - chr2_4:15373809-15373828 None:intergenic 25.0%
! TTGAACAAAAAGTTGTTGAA+GGG + chr2_4:15374263-15374282 Msa0317480:CDS 25.0%
!!! AGATAGGCTATAATTTGTTA+GGG - chr2_4:15374547-15374566 None:intergenic 25.0%
!!! AAGATAGGCTATAATTTGTT+AGG - chr2_4:15374548-15374567 None:intergenic 25.0%
! TAAGCCAAAATAAAAGAAGT+GGG - chr2_4:15374585-15374604 None:intergenic 25.0%
! ATAAGCCAAAATAAAAGAAG+TGG - chr2_4:15374586-15374605 None:intergenic 25.0%
! ATGTTTAGATCTTCACTTTA+TGG + chr2_4:15374616-15374635 Msa0317480:three_prime_UTR 25.0%
! CTACTTAAACATTGTTGATA+AGG + chr2_4:15374713-15374732 Msa0317480:three_prime_UTR 25.0%
!! ACTTAAACATTGTTGATAAG+GGG + chr2_4:15374715-15374734 Msa0317480:three_prime_UTR 25.0%
!!! TGATCATGTTGTTTTTGTGT+TGG - chr2_4:15372168-15372187 None:intergenic 30.0%
AAAATTCTCTTTGTTGGAGA+GGG + chr2_4:15372306-15372325 Msa0317480:CDS 30.0%
! TTTCTCTCTTTGTTTAGCTA+GGG + chr2_4:15372338-15372357 Msa0317480:CDS 30.0%
TAATGGAATGAGTAATGCAA+GGG + chr2_4:15372528-15372547 Msa0317480:CDS 30.0%
AAAGGAAACATTACTGAATC+TGG - chr2_4:15372699-15372718 None:intergenic 30.0%
! TTGCCCAATAAATTTTGGAT+AGG - chr2_4:15372880-15372899 None:intergenic 30.0%
!! TGATGATTTTTCAGCTGATT+TGG - chr2_4:15373056-15373075 None:intergenic 30.0%
! CTCTCAATAAATAGCCATTT+TGG - chr2_4:15373196-15373215 None:intergenic 30.0%
AAATGTATATGACCACAACT+TGG - chr2_4:15373372-15373391 None:intergenic 30.0%
AGTATATTTGACCTTAATCG+TGG + chr2_4:15373410-15373429 Msa0317480:intron 30.0%
TCTGTCTATGTTTAACATCA+AGG - chr2_4:15373459-15373478 None:intergenic 30.0%
GTAGTTGAATGACTCAAAAA+TGG - chr2_4:15373564-15373583 None:intergenic 30.0%
!! ACACTAATTTGGCTTGATTT+AGG - chr2_4:15373632-15373651 None:intergenic 30.0%
GCAATAGAAATTAAGTAGGT+AGG + chr2_4:15373653-15373672 Msa0317480:intron 30.0%
GTGCTTCCTAATAGAATTTA+AGG + chr2_4:15373710-15373729 Msa0317480:intron 30.0%
! GGTTTGACTAAATTTTACTC+GGG + chr2_4:15373746-15373765 Msa0317480:intron 30.0%
CAAATATTGTCAACTAGTAG+CGG - chr2_4:15374059-15374078 None:intergenic 30.0%
GGTACATAAATACTGTATAG+CGG + chr2_4:15374078-15374097 Msa0317480:intron 30.0%
TGTCTCAAGAATAGTTTGAA+AGG - chr2_4:15374197-15374216 None:intergenic 30.0%
GTTGAACAAAAAGTTGTTGA+AGG + chr2_4:15374262-15374281 Msa0317480:CDS 30.0%
TGAACAAAAAGTTGTTGAAG+GGG + chr2_4:15374264-15374283 Msa0317480:CDS 30.0%
ATTCATGTAACTCACAAAGA+GGG + chr2_4:15374332-15374351 Msa0317480:CDS 30.0%
GTAAGAAAAGCAGAAAAAAG+AGG + chr2_4:15374380-15374399 Msa0317480:CDS 30.0%
!! TAGTCCCACTTCTTTTATTT+TGG + chr2_4:15374578-15374597 Msa0317480:three_prime_UTR 30.0%
ATCATCATTGCAGATGAATA+TGG - chr2_4:15374830-15374849 None:intergenic 30.0%
! TATTGATTGGTCATTAGGGT+CGG + chr2_4:15372077-15372096 Msa0317480:five_prime_UTR 35.0%
CAAAATTCTCTTTGTTGGAG+AGG + chr2_4:15372305-15372324 Msa0317480:CDS 35.0%
! CTTTCTCTCTTTGTTTAGCT+AGG + chr2_4:15372337-15372356 Msa0317480:CDS 35.0%
! GTAATGGAATGAGTAATGCA+AGG + chr2_4:15372527-15372546 Msa0317480:CDS 35.0%
ATTTCTTTCTCAAGACTCAG+AGG + chr2_4:15372611-15372630 Msa0317480:CDS 35.0%
AGTAACTATTCTCAGGGTAA+AGG - chr2_4:15372675-15372694 None:intergenic 35.0%
! GCTCTTTGCCCAATAAATTT+TGG - chr2_4:15372885-15372904 None:intergenic 35.0%
! TATTGGGCAAAGAGCAATTT+TGG + chr2_4:15372890-15372909 Msa0317480:intron 35.0%
TTATGACTACTATGACATCG+AGG - chr2_4:15372930-15372949 None:intergenic 35.0%
ATGGGGAATACTATGACTAA+CGG + chr2_4:15373096-15373115 Msa0317480:intron 35.0%
! TGATAGTGAGGTAAACATAC+AGG + chr2_4:15373168-15373187 Msa0317480:intron 35.0%
GATAGTGAGGTAAACATACA+GGG + chr2_4:15373169-15373188 Msa0317480:intron 35.0%
TAAACATACAGGGACCAAAA+TGG + chr2_4:15373179-15373198 Msa0317480:intron 35.0%
CATCAAAAACCTTGATGTCA+TGG + chr2_4:15373345-15373364 Msa0317480:intron 35.0%
TGGCTTGATTTAGGAAATAG+AGG - chr2_4:15373623-15373642 None:intergenic 35.0%
TCGTGGTTTCCTTTCAATAA+GGG + chr2_4:15373855-15373874 Msa0317480:intron 35.0%
AAAGTTGCACAACAACTTCA+TGG - chr2_4:15373905-15373924 None:intergenic 35.0%
TCCATCAATGTCGTTAATTG+TGG + chr2_4:15373966-15373985 Msa0317480:intron 35.0%
CCATCAATGTCGTTAATTGT+GGG + chr2_4:15373967-15373986 Msa0317480:intron 35.0%
CGCTACTAGTTGACAATATT+TGG + chr2_4:15374057-15374076 Msa0317480:intron 35.0%
TATTCTTGAGACACAGTGTA+TGG + chr2_4:15374204-15374223 Msa0317480:intron 35.0%
! AACTTTTTGTTCAACCTGCT+TGG - chr2_4:15374257-15374276 None:intergenic 35.0%
AAAGTTGTTGAAGGGGTTTA+TGG + chr2_4:15374271-15374290 Msa0317480:CDS 35.0%
!! AAAGGCACTAGTAAAGAAAG+AGG + chr2_4:15374301-15374320 Msa0317480:CDS 35.0%
! AAGGCACTAGTAAAGAAAGA+GGG + chr2_4:15374302-15374321 Msa0317480:CDS 35.0%
GATTCATGTAACTCACAAAG+AGG + chr2_4:15374331-15374350 Msa0317480:CDS 35.0%
!! ACAAGATCCCATTTGTTGTA+TGG - chr2_4:15374362-15374381 None:intergenic 35.0%
! GGATAGTCATCTTTGAAGAA+TGG - chr2_4:15374428-15374447 None:intergenic 35.0%
TTCTTCAAAGATGACTATCC+TGG + chr2_4:15374428-15374447 Msa0317480:CDS 35.0%
TCAGATTTATCTTTCCCTGT+TGG + chr2_4:15374482-15374501 Msa0317480:CDS 35.0%
CAGATTTATCTTTCCCTGTT+GGG + chr2_4:15374483-15374502 Msa0317480:CDS 35.0%
AGATTTATCTTTCCCTGTTG+GGG + chr2_4:15374484-15374503 Msa0317480:CDS 35.0%
AAGGGGTTTGTAATGAATGA+TGG + chr2_4:15374732-15374751 Msa0317480:three_prime_UTR 35.0%
TTAGTCCAAAGTCTAAACTC+TGG + chr2_4:15374798-15374817 Msa0317480:three_prime_UTR 35.0%
CATTTCCAGAGTTTAGACTT+TGG - chr2_4:15374806-15374825 None:intergenic 35.0%
! TCAGTGACAGTGTTAAGTTG+AGG - chr2_4:15372192-15372211 None:intergenic 40.0%
CATCATCGAACACAGCAAAA+TGG + chr2_4:15372259-15372278 Msa0317480:CDS 40.0%
!! CTTTGTTTAGCTAGGGCTTT+TGG + chr2_4:15372345-15372364 Msa0317480:CDS 40.0%
AATCAAGGGAAGTGGCTATA+AGG - chr2_4:15372383-15372402 None:intergenic 40.0%
TGCATTACCCTGAGAATCAA+GGG - chr2_4:15372397-15372416 None:intergenic 40.0%
TTGCATTACCCTGAGAATCA+AGG - chr2_4:15372398-15372417 None:intergenic 40.0%
TCTGGCAGTAACTATTCTCA+GGG - chr2_4:15372681-15372700 None:intergenic 40.0%
ATCTGGCAGTAACTATTCTC+AGG - chr2_4:15372682-15372701 None:intergenic 40.0%
TAACAGACTGCAAAGTGAGT+GGG - chr2_4:15372956-15372975 None:intergenic 40.0%
!!! TTTTCAGCTGATTTGGCAAG+TGG - chr2_4:15373049-15373068 None:intergenic 40.0%
! TATGACTAACGGATGCAGAT+TGG + chr2_4:15373107-15373126 Msa0317480:intron 40.0%
! ATGACTAACGGATGCAGATT+GGG + chr2_4:15373108-15373127 Msa0317480:intron 40.0%
!! GGGGGGTTATTTTGTGTTTT+TGG + chr2_4:15373128-15373147 Msa0317480:intron 40.0%
! TCACGACTACTATGATAGTG+AGG + chr2_4:15373156-15373175 Msa0317480:intron 40.0%
CACAGTTACATTGTGATCCA+TGG + chr2_4:15373264-15373283 Msa0317480:intron 40.0%
TGTGATCCATGGTATTACAG+AGG + chr2_4:15373275-15373294 Msa0317480:intron 40.0%
CACAAACCTCTGTAATACCA+TGG - chr2_4:15373284-15373303 None:intergenic 40.0%
CATGGTATTACAGAGGTTTG+TGG + chr2_4:15373282-15373301 Msa0317480:intron 40.0%
!!! GCAGACCTTTTTTAAAACCC+TGG + chr2_4:15373487-15373506 Msa0317480:intron 40.0%
CTCGTGGTTTCCTTTCAATA+AGG + chr2_4:15373854-15373873 Msa0317480:intron 40.0%
CGTGGTTTCCTTTCAATAAG+GGG + chr2_4:15373856-15373875 Msa0317480:intron 40.0%
TCCTTTCAATAAGGGGTTCT+TGG + chr2_4:15373863-15373882 Msa0317480:intron 40.0%
TCCAAGAACCCCTTATTGAA+AGG - chr2_4:15373867-15373886 None:intergenic 40.0%
CCCACAATTAACGACATTGA+TGG - chr2_4:15373970-15373989 None:intergenic 40.0%
ATGTCGTTAATTGTGGGTAG+CGG + chr2_4:15373973-15373992 Msa0317480:intron 40.0%
TCAACTAGTAGCGGCTATAA+TGG - chr2_4:15374050-15374069 None:intergenic 40.0%
CTGATCCATGTGTTCTTGTT+TGG + chr2_4:15374132-15374151 Msa0317480:intron 40.0%
ATGCACCAAACAAGAACACA+TGG - chr2_4:15374140-15374159 None:intergenic 40.0%
!! TGTTCTTGTTTGGTGCATTG+TGG + chr2_4:15374142-15374161 Msa0317480:intron 40.0%
TGAATTTGTGACAACCAAGC+AGG + chr2_4:15374240-15374259 Msa0317480:intron 40.0%
CACTAGTAAAGAAAGAGGGA+GGG + chr2_4:15374306-15374325 Msa0317480:CDS 40.0%
ACTAGTAAAGAAAGAGGGAG+GGG + chr2_4:15374307-15374326 Msa0317480:CDS 40.0%
AGGGTGATCCATACAACAAA+TGG + chr2_4:15374351-15374370 Msa0317480:CDS 40.0%
GGGTGATCCATACAACAAAT+GGG + chr2_4:15374352-15374371 Msa0317480:CDS 40.0%
! CTTTGAAGAATGGAACAGCT+TGG - chr2_4:15374418-15374437 None:intergenic 40.0%
TATGACAACAAGAGAGCTCA+TGG + chr2_4:15374452-15374471 Msa0317480:CDS 40.0%
GAGCTTGAACTTGTAAGTAC+TGG - chr2_4:15374513-15374532 None:intergenic 40.0%
!! ATTTGTTAGGGAGAGGTGTT+TGG - chr2_4:15374535-15374554 None:intergenic 40.0%
!! GGCTATAATTTGTTAGGGAG+AGG - chr2_4:15374542-15374561 None:intergenic 40.0%
GACTAGAAGTTGGCAAAGAT+AGG - chr2_4:15374563-15374582 None:intergenic 40.0%
AAAGAAGTGGGACTAGAAGT+TGG - chr2_4:15374573-15374592 None:intergenic 40.0%
GCAAGGGAACTTGAAGAAAG+AGG + chr2_4:15372544-15372563 Msa0317480:CDS 45.0%
GGGTAAAGGAATCCAACATG+AGG - chr2_4:15372661-15372680 None:intergenic 45.0%
AACAGACTGCAAAGTGAGTG+GGG - chr2_4:15372955-15372974 None:intergenic 45.0%
CTAACAGACTGCAAAGTGAG+TGG - chr2_4:15372957-15372976 None:intergenic 45.0%
! TGACTAACGGATGCAGATTG+GGG + chr2_4:15373109-15373128 Msa0317480:intron 45.0%
TGCGATCTTAATCTCAGTCG+CGG + chr2_4:15373319-15373338 Msa0317480:intron 45.0%
CAACTTGGACCATGACATCA+AGG - chr2_4:15373357-15373376 None:intergenic 45.0%
TGATGTCATGGTCCAAGTTG+TGG + chr2_4:15373357-15373376 Msa0317480:intron 45.0%
CAATAAGGGGTTCTTGGAAC+TGG + chr2_4:15373869-15373888 Msa0317480:intron 45.0%
!! GTTTGGTGCATTGTGGACTA+TGG + chr2_4:15374149-15374168 Msa0317480:intron 45.0%
GGGGTTTATGGCAAATGCAA+AGG + chr2_4:15374283-15374302 Msa0317480:CDS 45.0%
GCACTAGTAAAGAAAGAGGG+AGG + chr2_4:15374305-15374324 Msa0317480:CDS 45.0%
! GCTCTCTTGTTGTCATAACC+AGG - chr2_4:15374449-15374468 None:intergenic 45.0%
AGCCACTTCCCTTGATTCTC+AGG + chr2_4:15372386-15372405 Msa0317480:CDS 50.0%
GCCACTTCCCTTGATTCTCA+GGG + chr2_4:15372387-15372406 Msa0317480:CDS 50.0%
ACCCTGAGAATCAAGGGAAG+TGG - chr2_4:15372391-15372410 None:intergenic 50.0%
GACATCGAGGTGCACATTCA+AGG - chr2_4:15372917-15372936 None:intergenic 50.0%
ACAGACTGCAAAGTGAGTGG+GGG - chr2_4:15372954-15372973 None:intergenic 50.0%
!! AGCTGATTTGGCAAGTGGAG+AGG - chr2_4:15373044-15373063 None:intergenic 50.0%
! GACTAACGGATGCAGATTGG+GGG + chr2_4:15373110-15373129 Msa0317480:intron 50.0%
! ACTAACGGATGCAGATTGGG+GGG + chr2_4:15373111-15373130 Msa0317480:intron 50.0%
TCGCAACGCAACCACGATTA+AGG - chr2_4:15373424-15373443 None:intergenic 50.0%
! ATCAGCCAAGTTAGCTCTCG+TGG + chr2_4:15373838-15373857 Msa0317480:intron 50.0%
GGAAACCACGAGAGCTAACT+TGG - chr2_4:15373846-15373865 None:intergenic 50.0%
!! AAGTACTGGCTTCCCCAACA+GGG - chr2_4:15374499-15374518 None:intergenic 50.0%
!! TAAGTACTGGCTTCCCCAAC+AGG - chr2_4:15374500-15374519 None:intergenic 50.0%
CAGACTGCAAAGTGAGTGGG+GGG - chr2_4:15372953-15372972 None:intergenic 55.0%
TGGCAAGTGGAGAGGCAAGT+GGG - chr2_4:15373036-15373055 None:intergenic 55.0%
TTGGCAAGTGGAGAGGCAAG+TGG - chr2_4:15373037-15373056 None:intergenic 55.0%
!! GGGTAGCGGAGAATAGCAAG+CGG + chr2_4:15373987-15374006 Msa0317480:intron 55.0%
ATGGCGCTGTAGCACTGCTG+CGG - chr2_4:15374031-15374050 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
chr2_4 gene 15371971 15374888 15371971 ID=Msa0317480;Name=Msa0317480
chr2_4 mRNA 15371971 15374888 15371971 ID=Msa0317480-mRNA-1;Parent=Msa0317480;Name=Msa0317480-mRNA-1;_AED=0.00;_eAED=0.00;_QI=158|1|1|1|1|1|3|329|258
chr2_4 exon 15371971 15372408 15371971 ID=Msa0317480-mRNA-1:exon:5696;Parent=Msa0317480-mRNA-1
chr2_4 exon 15372506 15372704 15372506 ID=Msa0317480-mRNA-1:exon:5697;Parent=Msa0317480-mRNA-1
chr2_4 exon 15374262 15374888 15374262 ID=Msa0317480-mRNA-1:exon:5698;Parent=Msa0317480-mRNA-1
chr2_4 five_prime_UTR 15371971 15372128 15371971 ID=Msa0317480-mRNA-1:five_prime_utr;Parent=Msa0317480-mRNA-1
chr2_4 CDS 15372129 15372408 15372129 ID=Msa0317480-mRNA-1:cds;Parent=Msa0317480-mRNA-1
chr2_4 CDS 15372506 15372704 15372506 ID=Msa0317480-mRNA-1:cds;Parent=Msa0317480-mRNA-1
chr2_4 CDS 15374262 15374559 15374262 ID=Msa0317480-mRNA-1:cds;Parent=Msa0317480-mRNA-1
chr2_4 three_prime_UTR 15374560 15374888 15374560 ID=Msa0317480-mRNA-1:three_prime_utr;Parent=Msa0317480-mRNA-1
Gene Sequence

>Msa0317480

ATGATTGAATATCAATTGATCAAAATCAAAATGAGTCCAACACAAAAACAACATGATCATCCTCAACTTAACACTGTCACTGACACTGATGATGAAGAACAAGAATCAGAGTCAGAAAAAGAAGAAGAATCATCATCGAACACAGCAAAATGGAAAAAACACTACTCATCAAAACACAAAATTCTCTTTGTTGGAGAGGGTGATTTTTCTTTCTCTCTTTGTTTAGCTAGGGCTTTTGGTTCTGCTCATAACCTTATAGCCACTTCCCTTGATTCTCAGGAGAAAATTGAAAAGAAGTATAGTAATGGAATGAGTAATGCAAGGGAACTTGAAGAAAGAGGTTGTATTGTTCTTTATGATGTTGATGTTAAGGTGATGAGTCAGCATTTCTTTCTCAAGACTCAGAGGTTTGATCGTGTTGTTTATAATTTTCCTCATGTTGGATTCCTTTACCCTGAGAATAGTTACTGCCAGATTCAGTTGAACAAAAAGTTGTTGAAGGGGTTTATGGCAAATGCAAAGGCACTAGTAAAGAAAGAGGGAGGGGAGATTCATGTAACTCACAAAGAGGGTGATCCATACAACAAATGGGATCTTGTAAGAAAAGCAGAAAAAAGAGGACTGTTCTTACACCAAGCTGTTCCATTCTTCAAAGATGACTATCCTGGTTATGACAACAAGAGAGCTCATGGAAAGTTATCAGATTTATCTTTCCCTGTTGGGGAAGCCAGTACTTACAAGTTCAAGCTCCAAACACCTCTCCCTAACAAATTATAG
Protein sequence

>Msa0317480

MIEYQLIKIKMSPTQKQHDHPQLNTVTDTDDEEQESESEKEEESSSNTAKWKKHYSSKHKILFVGEGDFSFSLCLARAFGSAHNLIATSLDSQEKIEKKYSNGMSNARELEERGCIVLYDVDVKVMSQHFFLKTQRFDRVVYNFPHVGFLYPENSYCQIQLNKKLLKGFMANAKALVKKEGGEIHVTHKEGDPYNKWDLVRKAEKRGLFLHQAVPFFKDDYPGYDNKRAHGKLSDLSFPVGEASTYKFKLQTPLPNKL*