AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1396410

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1396410 MtrunA17_Chr7g0268991 100.000 164 0 0 1 164 157 320 5.40e-122 346
Msa1396410 MtrunA17_Chr2g0314601 25.767 163 110 4 1 161 169 322 4.43e-12 63.2
Msa1396410 MtrunA17_Chr1g0147841 27.879 165 104 5 1 161 167 320 5.77e-12 62.8
Msa1396410 MtrunA17_Chr8g0359401 26.380 163 109 4 1 161 169 322 7.87e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1396410 AT1G73250.1 85.714 161 23 0 2 162 160 320 3.61e-105 304
Msa1396410 AT1G17890.2 84.756 164 25 0 1 164 154 317 9.03e-105 303
Msa1396410 AT1G17890.3 84.756 164 25 0 1 164 154 317 9.03e-105 303
Msa1396410 AT1G17890.1 84.756 164 25 0 1 164 162 325 1.08e-104 303
Msa1396410 AT3G14790.2 28.659 164 104 6 1 161 172 325 3.59e-14 69.7
Msa1396410 AT3G14790.1 28.659 164 104 6 1 161 169 322 4.14e-14 69.3
Msa1396410 AT1G53500.1 26.994 163 108 4 1 161 171 324 5.04e-12 63.2
Msa1396410 AT1G78570.1 25.153 163 111 4 1 161 169 322 5.88e-12 63.2

Find 141 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGAAGATGCTTTGCTTAC+TGG 0.149882 tig0022022:-77280 Msa1396410:five_prime_UTR
ATTGGTTGAGGTGCAAATTT+AGG 0.159427 tig0022022:+77304 None:intergenic
GAGCTATCCATCAGCTTTCT+TGG 0.194906 tig0022022:+76824 None:intergenic
CACAAGTGGGATGCAATTTC+AGG 0.203663 tig0022022:-77178 Msa1396410:CDS
TTTGTAGCAATTCTCAAATA+TGG 0.237057 tig0022022:-78392 Msa1396410:five_prime_UTR
AACAATAACCCTTACTGTTT+TGG 0.240454 tig0022022:+78365 None:intergenic
CCATTCATTTGTGGGCTCTA+AGG 0.251483 tig0022022:+77255 None:intergenic
ATTCTCTAAGTACCACTTAT+AGG 0.298342 tig0022022:+76742 None:intergenic
GAGGTAGTGGTGTGGGGTAC+GGG 0.306076 tig0022022:-77046 Msa1396410:CDS
AACCTATATAAAACTTGATA+TGG 0.311729 tig0022022:+76684 None:intergenic
CCAGTAAGCAAAGCATCTTC+AGG 0.327068 tig0022022:+77280 None:intergenic
TGGAAAGGAGGTTACAATTA+AGG 0.329079 tig0022022:-76924 Msa1396410:CDS
GGAGGTTACAATTAAGGAAT+TGG 0.338695 tig0022022:-76918 Msa1396410:CDS
AATGGATCATACAATGTAAC+TGG 0.346295 tig0022022:+76619 None:intergenic
TATTGGAGCGAGTCTGAGTT+GGG 0.359537 tig0022022:-76411 Msa1396410:three_prime_UTR
ATGTCAAGCAATGATTCTTC+TGG 0.362973 tig0022022:-76721 Msa1396410:exon
GGGATGCCGACCAACTTATA+CGG 0.369504 tig0022022:-77157 Msa1396410:CDS
TTGGTTGAGGTGCAAATTTA+GGG 0.370655 tig0022022:+77305 None:intergenic
CATTCATTTGTGGGCTCTAA+GGG 0.394688 tig0022022:+77256 None:intergenic
AAACTGCTAATGGGTGTTCA+TGG 0.397523 tig0022022:+76501 None:intergenic
CAATGACATTGGTCTGGATC+TGG 0.398176 tig0022022:+77386 None:intergenic
AGCTTTCTTGGTGTCCCATC+GGG 0.401801 tig0022022:+76836 None:intergenic
AGGTAGTGGTGTGGGGTACG+GGG 0.403323 tig0022022:-77045 Msa1396410:CDS
TGTTGGTCTATCATAGCTTC+TGG 0.405355 tig0022022:-76314 Msa1396410:three_prime_UTR
AATAACTCATCTTAGTGAAA+TGG 0.407614 tig0022022:+76658 None:intergenic
TAGCTTCTGGTAATTAAAGT+TGG 0.409180 tig0022022:-76301 Msa1396410:three_prime_UTR
GGAGGTAGTGGTGTGGGGTA+CGG 0.416144 tig0022022:-77047 Msa1396410:CDS
TTGTAGCAATTCTCAAATAT+GGG 0.416582 tig0022022:-78391 Msa1396410:five_prime_UTR
TCGGGATGGAAATTATCATT+TGG 0.418888 tig0022022:+77133 None:intergenic
GGTGAATCGGTCTCCGGTGG+TGG 0.423844 tig0022022:+78639 None:intergenic
AGCTCAAAACTTGCAGCCCT+TGG 0.432660 tig0022022:-76806 Msa1396410:CDS
GGTCAGATCTGAAAGGATTC+CGG 0.434451 tig0022022:+78664 None:intergenic
CGAATCAGCTACTGAGCTAT+TGG 0.436756 tig0022022:+78692 None:intergenic
TATGCCATTGCCAAGATTGC+CGG 0.438517 tig0022022:-77232 Msa1396410:five_prime_UTR
CAGCTTTCTTGGTGTCCCAT+CGG 0.440823 tig0022022:+76835 None:intergenic
TTATCTTGTTCAAGATATCA+TGG 0.441075 tig0022022:-76547 Msa1396410:three_prime_UTR
AGGAAGTGAAATGGGTTTGT+GGG 0.447678 tig0022022:+78579 None:intergenic
TCTTCTACCGTTTAATTGAC+AGG 0.449371 tig0022022:-76349 Msa1396410:three_prime_UTR
GCGAGACAAAGGAAGTGAAA+TGG 0.457266 tig0022022:+78570 None:intergenic
GGAGTTGAGCATTTGAATGT+AGG 0.457314 tig0022022:-76950 Msa1396410:CDS
GGGTACGGGGAGTCCACTTA+GGG 0.460892 tig0022022:-77032 Msa1396410:CDS
TGAATGAAACTCCGGTGAAT+CGG 0.462794 tig0022022:+78626 None:intergenic
TGCAAAGGAGGTAGTGGTGT+GGG 0.465275 tig0022022:-77053 Msa1396410:CDS
TCATCGAGGTCTTGTTGGTT+CGG 0.467475 tig0022022:-77608 Msa1396410:five_prime_UTR
AATCGAGGAGGGTGCAGTGC+AGG 0.470403 tig0022022:-78790 Msa1396410:five_prime_UTR
ACCAACAAGACCTCGATGAC+CGG 0.471640 tig0022022:+77612 None:intergenic
GGCATGGATACCACCAACTT+TGG 0.472668 tig0022022:+77447 None:intergenic
AGCAAAGCATCTTCAGGGAT+TGG 0.474197 tig0022022:+77286 None:intergenic
GGGGTACGGGGAGTCCACTT+AGG 0.476642 tig0022022:-77033 Msa1396410:CDS
CAGAATCAATGACATTGGTC+TGG 0.477646 tig0022022:+77380 None:intergenic
AGGCTTCGTGGATGCAAAGA+AGG 0.478791 tig0022022:+77492 None:intergenic
GGAATTCTGCTGCCACAGTT+TGG 0.479103 tig0022022:-76280 Msa1396410:three_prime_UTR
CCGGAGTTTCATTCACCCGT+TGG 0.480531 tig0022022:-78618 Msa1396410:five_prime_UTR
GAGTTGAGCATTTGAATGTA+GGG 0.483402 tig0022022:-76949 Msa1396410:CDS
ATCATTTGGTCCGTATAAGT+TGG 0.484602 tig0022022:+77147 None:intergenic
ATGGCATACCATTCATTTGT+GGG 0.486092 tig0022022:+77247 None:intergenic
TATGGGAAGCCAAAACAGTA+AGG 0.486587 tig0022022:-78374 Msa1396410:five_prime_UTR
CAAAACTTGCAGCCCTTGGT+TGG 0.488475 tig0022022:-76802 Msa1396410:CDS
GTTTCATTCACCCGTTGGAT+CGG 0.490068 tig0022022:-78613 Msa1396410:five_prime_UTR
AAGGAAGTGAAATGGGTTTG+TGG 0.491120 tig0022022:+78578 None:intergenic
GTATTGGAGCGAGTCTGAGT+TGG 0.491249 tig0022022:-76412 Msa1396410:three_prime_UTR
CAGATCTGACCTTCCACCAC+CGG 0.492159 tig0022022:-78652 Msa1396410:five_prime_UTR
CGAGACAAAGGAAGTGAAAT+GGG 0.493690 tig0022022:+78571 None:intergenic
ATCAGCAGGATAGGTGTTGT+TGG 0.496759 tig0022022:+77426 None:intergenic
AATGACAAACTCAGGCTTCG+TGG 0.498096 tig0022022:+77480 None:intergenic
GCATCTTCAGGGATTGGTTG+AGG 0.503925 tig0022022:+77292 None:intergenic
GTGTTCATGGTCTTTAACAA+TGG 0.505731 tig0022022:+76514 None:intergenic
ATGCCATTGCCAAGATTGCC+GGG 0.506721 tig0022022:-77231 Msa1396410:five_prime_UTR
GAATGTAGGGAGTGGAAAGG+AGG 0.511105 tig0022022:-76936 Msa1396410:CDS
CCATCCTTAAGTGAGACTTT+CGG 0.511365 tig0022022:+76776 None:intergenic
GTTCGCAAGCTGACTCAACT+CGG 0.514389 tig0022022:-77580 Msa1396410:five_prime_UTR
AAAAGAAAATCCGATCCAAC+GGG 0.518860 tig0022022:+78603 None:intergenic
CGAGTCTGAGTTGGGGCACA+AGG 0.519988 tig0022022:-76403 Msa1396410:three_prime_UTR
CTATGCCTGCTTGCTTGTGT+AGG 0.523923 tig0022022:+78843 None:intergenic
CAGGATAGGTGTTGTTGGCA+TGG 0.526132 tig0022022:+77431 None:intergenic
ACAAGTGGGATGCAATTTCA+GGG 0.528356 tig0022022:-77177 Msa1396410:CDS
CCTTAGAGCCCACAAATGAA+TGG 0.530586 tig0022022:-77255 Msa1396410:five_prime_UTR
ATTGTAGCTGCTGCCAAAGT+TGG 0.538288 tig0022022:-77460 Msa1396410:five_prime_UTR
GATCCCGGCAATCTTGGCAA+TGG 0.538775 tig0022022:+77228 None:intergenic
GGTCTGGATCTGGAGGTTGA+TGG 0.540673 tig0022022:+77396 None:intergenic
AATCGGTCTCCGGTGGTGGA+AGG 0.542203 tig0022022:+78643 None:intergenic
CCGAAAGTCTCACTTAAGGA+TGG 0.543510 tig0022022:-76776 Msa1396410:CDS
TGAAACCTCCTCATCAAAGC+AGG 0.544927 tig0022022:+77094 None:intergenic
CAACGGTGCAAAGGAGGTAG+TGG 0.546785 tig0022022:-77059 Msa1396410:CDS
CAGTAAGCAAAGCATCTTCA+GGG 0.549758 tig0022022:+77281 None:intergenic
CAGTAGCTGATTCGATCTGC+CGG 0.550955 tig0022022:-78683 Msa1396410:five_prime_UTR
AACTCCGGTGAATCGGTCTC+CGG 0.553123 tig0022022:+78633 None:intergenic
ACCACCGGAGACCGATTCAC+CGG 0.555662 tig0022022:-78637 Msa1396410:five_prime_UTR
GAGTCAATGAAGGAAGTTGT+GGG 0.556089 tig0022022:-76893 Msa1396410:CDS
TTGATGGCAATGAAATCAGC+AGG 0.556836 tig0022022:+77412 None:intergenic
GGAATTGGCTGAGTCAATGA+AGG 0.558355 tig0022022:-76903 Msa1396410:CDS
TTCGCAAGCTGACTCAACTC+GGG 0.561081 tig0022022:-77579 Msa1396410:five_prime_UTR
AATGGCATACCATTCATTTG+TGG 0.562972 tig0022022:+77246 None:intergenic
AGATGCAGTTGTCTTTATGA+TGG 0.567147 tig0022022:-76984 Msa1396410:CDS
GACACCGAAAGTCTCACTTA+AGG 0.568664 tig0022022:-76780 Msa1396410:CDS
GGCAATGAAATCAGCAGGAT+AGG 0.569157 tig0022022:+77417 None:intergenic
TGGGACACCAAGAAAGCTGA+TGG 0.571175 tig0022022:-76831 Msa1396410:CDS
GAGCATTTGAATGTAGGGAG+TGG 0.573638 tig0022022:-76944 Msa1396410:CDS
CTACACAAGCAAGCAGGCAT+AGG 0.576067 tig0022022:-78842 Msa1396410:five_prime_UTR
ACAGGAGGAAAACGTTGTGT+TGG 0.579417 tig0022022:-76331 Msa1396410:three_prime_UTR
CAAATGACACAAGAATAACT+TGG 0.579888 tig0022022:+76460 None:intergenic
GTAGCTGCTGCCAAAGTTGG+TGG 0.582192 tig0022022:-77457 Msa1396410:five_prime_UTR
ATTGATTCTGCTTATCGCAA+TGG 0.583179 tig0022022:-77367 Msa1396410:five_prime_UTR
GAGGAGGTTTCATGAGGCAA+AGG 0.584336 tig0022022:-77083 Msa1396410:CDS
TTGAGTCAGCTTGCGAACAA+TGG 0.584587 tig0022022:+77585 None:intergenic
TAAAAGAAAATCCGATCCAA+CGG 0.586666 tig0022022:+78602 None:intergenic
CATACAGAATTCAGCACAAG+TGG 0.592816 tig0022022:-77192 Msa1396410:CDS
TCCGGTGAATCGGTCTCCGG+TGG 0.593507 tig0022022:+78636 None:intergenic
CGAAAGTCTCACTTAAGGAT+GGG 0.593671 tig0022022:-76775 Msa1396410:CDS
GCAGCTACAATGACAAACTC+AGG 0.596147 tig0022022:+77472 None:intergenic
GCAAAGGAGGTAGTGGTGTG+GGG 0.596327 tig0022022:-77052 Msa1396410:CDS
TTTGGTCCGTATAAGTTGGT+CGG 0.596979 tig0022022:+77151 None:intergenic
AATTTCCTACACAAGCAAGC+AGG 0.598067 tig0022022:-78848 Msa1396410:five_prime_UTR
GACTTTCGGTGTCCAACCAA+GGG 0.600503 tig0022022:+76790 None:intergenic
GGCAAAGGTCAACGGTGCAA+AGG 0.605297 tig0022022:-77068 Msa1396410:CDS
TTTCATGAGGCAAAGGTCAA+CGG 0.606919 tig0022022:-77076 Msa1396410:CDS
AGACTTTCGGTGTCCAACCA+AGG 0.607421 tig0022022:+76789 None:intergenic
TAAAACTTGAAGCGAGACAA+AGG 0.607508 tig0022022:+78559 None:intergenic
ATAAGCAGAATCAATGACAT+TGG 0.607720 tig0022022:+77375 None:intergenic
GTGCAAAGGAGGTAGTGGTG+TGG 0.615768 tig0022022:-77054 Msa1396410:CDS
ATGACACAAGAATAACTTGG+TGG 0.616110 tig0022022:+76463 None:intergenic
GGCTTGTTGATACCTATAAG+TGG 0.616689 tig0022022:-76754 Msa1396410:CDS
GGAGCACAACAACAGTACAT+AGG 0.619172 tig0022022:-78821 Msa1396410:five_prime_UTR
TTTGAATGTAGGGAGTGGAA+AGG 0.619261 tig0022022:-76939 Msa1396410:CDS
AAAGGTCAACGGTGCAAAGG+AGG 0.620001 tig0022022:-77065 Msa1396410:CDS
ATGGGAAGCCAAAACAGTAA+GGG 0.622809 tig0022022:-78373 Msa1396410:five_prime_UTR
TCTAATCTTAAGAACTCATG+CGG 0.625073 tig0022022:-77548 Msa1396410:five_prime_UTR
ATGAGATGAGAGAATGAGTG+AGG 0.634514 tig0022022:+78721 None:intergenic
TTTGATGAGGAGGTTTCATG+AGG 0.636802 tig0022022:-77089 Msa1396410:CDS
CCAACGGGTGAATGAAACTC+CGG 0.651535 tig0022022:+78618 None:intergenic
AAATTTAGGGTAAATGCAAG+AGG 0.655370 tig0022022:+77318 None:intergenic
TGACATTGGTCTGGATCTGG+AGG 0.655686 tig0022022:+77389 None:intergenic
TCTACCGTTTAATTGACAGG+AGG 0.655863 tig0022022:-76346 Msa1396410:three_prime_UTR
TGAGTCAATGAAGGAAGTTG+TGG 0.655904 tig0022022:-76894 Msa1396410:CDS
GGTGGAAGGTCAGATCTGAA+AGG 0.658870 tig0022022:+78657 None:intergenic
GATGAAGCAACACAAGAGAA+CGG 0.659980 tig0022022:+78748 None:intergenic
ATTCATTTGTGGGCTCTAAG+GGG 0.661471 tig0022022:+77257 None:intergenic
ATTGGAGCGAGTCTGAGTTG+GGG 0.680747 tig0022022:-76410 Msa1396410:three_prime_UTR
TTTATGATGGAAAAGTACAG+TGG 0.685676 tig0022022:-76971 Msa1396410:CDS
TGACTCAAGCTACCAAACTG+TGG 0.689923 tig0022022:+76268 None:intergenic
ATACAGAATTCAGCACAAGT+GGG 0.695878 tig0022022:-77191 Msa1396410:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTGTTAAATTTTGAAA+TGG - tig0022022:76674-76693 Msa1396410:three_prime_UTR 15.0%
!!! TGATTTTTTTTTTTTTTTGC+AGG - tig0022022:76708-76727 Msa1396410:three_prime_UTR 15.0%
!!! TTTTTTTGCAGGATTTTATT+TGG - tig0022022:76719-76738 Msa1396410:exon 20.0%
!!! TTTATTTGGAATTTTGAAGT+TGG - tig0022022:76733-76752 Msa1396410:CDS 20.0%
!!! TTATTTTCTCAAATTACTAC+CGG - tig0022022:77006-77025 Msa1396410:CDS 20.0%
!! AACCTATATAAAACTTGATA+TGG + tig0022022:78473-78492 None:intergenic 20.0%
!!! ATGAGTTATTTTCTTTGTAT+TGG - tig0022022:78726-78745 Msa1396410:five_prime_UTR 20.0%
!! GAAAAAAAAAAATGCTGTAT+TGG - tig0022022:78773-78792 Msa1396410:five_prime_UTR 20.0%
! AAAATGACGAAACAACAAAA+GGG + tig0022022:76646-76665 None:intergenic 25.0%
! TAAAATGACGAAACAACAAA+AGG + tig0022022:76647-76666 None:intergenic 25.0%
! TTTGTAGCAATTCTCAAATA+TGG - tig0022022:76762-76781 Msa1396410:CDS 25.0%
! TTGTAGCAATTCTCAAATAT+GGG - tig0022022:76763-76782 Msa1396410:CDS 25.0%
! AACATTTAATGTGATCACAT+TGG + tig0022022:76988-77007 None:intergenic 25.0%
!!! CATTTGCTTTTGGTTTAATA+TGG - tig0022022:77189-77208 Msa1396410:CDS 25.0%
! TGGTTTAATATGGTATCAAT+AGG - tig0022022:77199-77218 Msa1396410:CDS 25.0%
!! CAGAATTTTACTAGATTCAA+AGG - tig0022022:77312-77331 Msa1396410:five_prime_UTR 25.0%
!! AATTTTACTAGATTCAAAGG+TGG - tig0022022:77315-77334 Msa1396410:five_prime_UTR 25.0%
! ATAACAATGCTAACTCATTA+TGG - tig0022022:77371-77390 Msa1396410:five_prime_UTR 25.0%
!!! GGTGAGAGAATTATTTTTAT+TGG - tig0022022:77425-77444 Msa1396410:five_prime_UTR 25.0%
!!! TGCCATATCAAGTTTTATAT+AGG - tig0022022:78468-78487 Msa1396410:intron 25.0%
! AATAACTCATCTTAGTGAAA+TGG + tig0022022:78499-78518 None:intergenic 25.0%
! ACGTAATTAGTACAGAAAAA+TGG + tig0022022:78556-78575 None:intergenic 25.0%
!! ATTTTTCTGTACTAATTACG+TGG - tig0022022:78555-78574 Msa1396410:intron 25.0%
!! TTTTTCTGTACTAATTACGT+GGG - tig0022022:78556-78575 Msa1396410:intron 25.0%
! TTATCTTGTTCAAGATATCA+TGG - tig0022022:78607-78626 Msa1396410:five_prime_UTR 25.0%
! ACACTAAAAAAACTGCTAAT+GGG + tig0022022:78665-78684 None:intergenic 25.0%
! AACACTAAAAAAACTGCTAA+TGG + tig0022022:78666-78685 None:intergenic 25.0%
ACATAGGTAAAAAACAATCG+AGG - tig0022022:76349-76368 Msa1396410:three_prime_UTR 30.0%
TAAAAGAAAATCCGATCCAA+CGG + tig0022022:76555-76574 None:intergenic 30.0%
AAATGACGAAACAACAAAAG+GGG + tig0022022:76645-76664 None:intergenic 30.0%
!! TTGTTTCGTCATTTTAGAAG+TGG - tig0022022:76651-76670 Msa1396410:three_prime_UTR 30.0%
!! TGTTTCGTCATTTTAGAAGT+GGG - tig0022022:76652-76671 Msa1396410:three_prime_UTR 30.0%
! AACAATAACCCTTACTGTTT+TGG + tig0022022:76792-76811 None:intergenic 30.0%
! ATGTCTTTGATCAAAAGTGT+CGG + tig0022022:76945-76964 None:intergenic 30.0%
!! TTAGCATTGTTATTGACTCT+TGG + tig0022022:77364-77383 None:intergenic 30.0%
!! TTTTGATGATAAATCTGCCA+AGG - tig0022022:77510-77529 Msa1396410:five_prime_UTR 30.0%
TCTAATCTTAAGAACTCATG+CGG - tig0022022:77606-77625 Msa1396410:five_prime_UTR 30.0%
ATAAGCAGAATCAATGACAT+TGG + tig0022022:77782-77801 None:intergenic 30.0%
!!! TGCTAAGAAGCTTTTGTTTT+TGG - tig0022022:77810-77829 Msa1396410:intron 30.0%
!!! GCTAAGAAGCTTTTGTTTTT+GGG - tig0022022:77811-77830 Msa1396410:intron 30.0%
! AAATTTAGGGTAAATGCAAG+AGG + tig0022022:77839-77858 None:intergenic 30.0%
! TTTATGATGGAAAAGTACAG+TGG - tig0022022:78183-78202 Msa1396410:intron 30.0%
ATTCTCTAAGTACCACTTAT+AGG + tig0022022:78415-78434 None:intergenic 30.0%
AATGGATCATACAATGTAAC+TGG + tig0022022:78538-78557 None:intergenic 30.0%
CAAATGACACAAGAATAACT+TGG + tig0022022:78697-78716 None:intergenic 30.0%
! TTTTCCTCCTGTCAATTAAA+CGG + tig0022022:78815-78834 None:intergenic 30.0%
TAGCTTCTGGTAATTAAAGT+TGG - tig0022022:78853-78872 Msa1396410:five_prime_UTR 30.0%
TAGGTAAAAAACAATCGAGG+AGG - tig0022022:76352-76371 Msa1396410:three_prime_UTR 35.0%
AGGTAAAAAACAATCGAGGA+GGG - tig0022022:76353-76372 Msa1396410:three_prime_UTR 35.0%
!! AGAGAACGGATTTTATGGAA+GGG + tig0022022:76395-76414 None:intergenic 35.0%
!! AAGAGAACGGATTTTATGGA+AGG + tig0022022:76396-76415 None:intergenic 35.0%
!! ACACAAGAGAACGGATTTTA+TGG + tig0022022:76400-76419 None:intergenic 35.0%
AAAAGAAAATCCGATCCAAC+GGG + tig0022022:76554-76573 None:intergenic 35.0%
TAAAACTTGAAGCGAGACAA+AGG + tig0022022:76598-76617 None:intergenic 35.0%
!! TTTGTCTCGCTTCAAGTTTT+AGG - tig0022022:76597-76616 Msa1396410:three_prime_UTR 35.0%
AATGACGAAACAACAAAAGG+GGG + tig0022022:76644-76663 None:intergenic 35.0%
TCGAAAAAAACGCTCAATTG+CGG + tig0022022:77121-77140 None:intergenic 35.0%
CCAAAAGCAAATGAACTCAA+GGG + tig0022022:77182-77201 None:intergenic 35.0%
ACCAAAAGCAAATGAACTCA+AGG + tig0022022:77183-77202 None:intergenic 35.0%
!!! TAGCTGTTTTTCCAGTTTGT+AGG - tig0022022:77224-77243 Msa1396410:exon 35.0%
TATAATCAGGACCTACAAAC+TGG + tig0022022:77238-77257 None:intergenic 35.0%
GTCAACGAGAACTTATAATC+AGG + tig0022022:77251-77270 None:intergenic 35.0%
AAAGATAGCGTAGTTCGTAT+AGG + tig0022022:77279-77298 None:intergenic 35.0%
TTGCCCTAAAAAAAGAAGAG+AGG - tig0022022:77403-77422 Msa1396410:five_prime_UTR 35.0%
TGCCCTAAAAAAAGAAGAGA+GGG - tig0022022:77404-77423 Msa1396410:five_prime_UTR 35.0%
!!! CACCCTCTCTTCTTTTTTTA+GGG + tig0022022:77409-77428 None:intergenic 35.0%
!!! TCACCCTCTCTTCTTTTTTT+AGG + tig0022022:77410-77429 None:intergenic 35.0%
! ATTGATTCTGCTTATCGCAA+TGG - tig0022022:77787-77806 Msa1396410:intron 35.0%
TTGGTTGAGGTGCAAATTTA+GGG + tig0022022:77852-77871 None:intergenic 35.0%
ATTGGTTGAGGTGCAAATTT+AGG + tig0022022:77853-77872 None:intergenic 35.0%
ATGGCATACCATTCATTTGT+GGG + tig0022022:77910-77929 None:intergenic 35.0%
AATGGCATACCATTCATTTG+TGG + tig0022022:77911-77930 None:intergenic 35.0%
ATACAGAATTCAGCACAAGT+GGG - tig0022022:77963-77982 Msa1396410:intron 35.0%
ATCATTTGGTCCGTATAAGT+TGG + tig0022022:78010-78029 None:intergenic 35.0%
TCGGGATGGAAATTATCATT+TGG + tig0022022:78024-78043 None:intergenic 35.0%
! AGGCAAAACGTGTGAATTTT+CGG + tig0022022:78043-78062 None:intergenic 35.0%
AGATGCAGTTGTCTTTATGA+TGG - tig0022022:78170-78189 Msa1396410:intron 35.0%
GAGTTGAGCATTTGAATGTA+GGG - tig0022022:78205-78224 Msa1396410:intron 35.0%
TGGAAAGGAGGTTACAATTA+AGG - tig0022022:78230-78249 Msa1396410:intron 35.0%
GGAGGTTACAATTAAGGAAT+TGG - tig0022022:78236-78255 Msa1396410:intron 35.0%
ATGTCAAGCAATGATTCTTC+TGG - tig0022022:78433-78452 Msa1396410:intron 35.0%
GTGTTCATGGTCTTTAACAA+TGG + tig0022022:78643-78662 None:intergenic 35.0%
ATGACACAAGAATAACTTGG+TGG + tig0022022:78694-78713 None:intergenic 35.0%
TCTTCTACCGTTTAATTGAC+AGG - tig0022022:78805-78824 Msa1396410:five_prime_UTR 35.0%
AATTTCCTACACAAGCAAGC+AGG - tig0022022:76306-76325 Msa1396410:three_prime_UTR 40.0%
GATGAAGCAACACAAGAGAA+CGG + tig0022022:76409-76428 None:intergenic 40.0%
ATGAGATGAGAGAATGAGTG+AGG + tig0022022:76436-76455 None:intergenic 40.0%
TGAATGAAACTCCGGTGAAT+CGG + tig0022022:76531-76550 None:intergenic 40.0%
! AGGAAGTGAAATGGGTTTGT+GGG + tig0022022:76578-76597 None:intergenic 40.0%
! AAGGAAGTGAAATGGGTTTG+TGG + tig0022022:76579-76598 None:intergenic 40.0%
CGAGACAAAGGAAGTGAAAT+GGG + tig0022022:76586-76605 None:intergenic 40.0%
TATGGGAAGCCAAAACAGTA+AGG - tig0022022:76780-76799 Msa1396410:CDS 40.0%
ATGGGAAGCCAAAACAGTAA+GGG - tig0022022:76781-76800 Msa1396410:CDS 40.0%
! CACATTGGTTGAAGTTGTGT+CGG + tig0022022:76973-76992 None:intergenic 40.0%
CTAAAGCAATCGCTTCTGAA+AGG - tig0022022:77079-77098 Msa1396410:CDS 40.0%
! CCCTTGAGTTCATTTGCTTT+TGG - tig0022022:77179-77198 Msa1396410:CDS 40.0%
!! TCTGCCAAGGTTTTTGTTTC+CGG - tig0022022:77523-77542 Msa1396410:five_prime_UTR 40.0%
ATGACCGGAAACAAAAACCT+TGG + tig0022022:77530-77549 None:intergenic 40.0%
! TTGATGGCAATGAAATCAGC+AGG + tig0022022:77745-77764 None:intergenic 40.0%
CAGAATCAATGACATTGGTC+TGG + tig0022022:77777-77796 None:intergenic 40.0%
! CAGTAAGCAAAGCATCTTCA+GGG + tig0022022:77876-77895 None:intergenic 40.0%
!! ATTCATTTGTGGGCTCTAAG+GGG + tig0022022:77900-77919 None:intergenic 40.0%
! CATTCATTTGTGGGCTCTAA+GGG + tig0022022:77901-77920 None:intergenic 40.0%
!! TGCTTGACACATTTTGATCC+CGG + tig0022022:77944-77963 None:intergenic 40.0%
CATACAGAATTCAGCACAAG+TGG - tig0022022:77962-77981 Msa1396410:intron 40.0%
ACAAGTGGGATGCAATTTCA+GGG - tig0022022:77977-77996 Msa1396410:intron 40.0%
!! TTTGGTCCGTATAAGTTGGT+CGG + tig0022022:78006-78025 None:intergenic 40.0%
!! AAACGTGTGAATTTTCGGGA+TGG + tig0022022:78038-78057 None:intergenic 40.0%
! GGCAAAACGTGTGAATTTTC+GGG + tig0022022:78042-78061 None:intergenic 40.0%
TTTGATGAGGAGGTTTCATG+AGG - tig0022022:78065-78084 Msa1396410:intron 40.0%
TTTCATGAGGCAAAGGTCAA+CGG - tig0022022:78078-78097 Msa1396410:intron 40.0%
! GTTTTTGCACGTTGACGATT+TGG - tig0022022:78146-78165 Msa1396410:intron 40.0%
GGAGTTGAGCATTTGAATGT+AGG - tig0022022:78204-78223 Msa1396410:intron 40.0%
TTTGAATGTAGGGAGTGGAA+AGG - tig0022022:78215-78234 Msa1396410:intron 40.0%
TGAGTCAATGAAGGAAGTTG+TGG - tig0022022:78260-78279 Msa1396410:intron 40.0%
GAGTCAATGAAGGAAGTTGT+GGG - tig0022022:78261-78280 Msa1396410:intron 40.0%
!! AAGGAAGTTGTGGGTTTTGA+AGG - tig0022022:78270-78289 Msa1396410:intron 40.0%
!! TTTTGAAGGCGATCTCGTTT+GGG - tig0022022:78284-78303 Msa1396410:intron 40.0%
CCATCCTTAAGTGAGACTTT+CGG + tig0022022:78381-78400 None:intergenic 40.0%
CGAAAGTCTCACTTAAGGAT+GGG - tig0022022:78379-78398 Msa1396410:five_prime_UTR 40.0%
!! GGCTTGTTGATACCTATAAG+TGG - tig0022022:78400-78419 Msa1396410:five_prime_UTR 40.0%
! AAACTGCTAATGGGTGTTCA+TGG + tig0022022:78656-78675 None:intergenic 40.0%
TCTACCGTTTAATTGACAGG+AGG - tig0022022:78808-78827 Msa1396410:five_prime_UTR 40.0%
! TGTTGGTCTATCATAGCTTC+TGG - tig0022022:78840-78859 Msa1396410:five_prime_UTR 40.0%
GGAGCACAACAACAGTACAT+AGG - tig0022022:76333-76352 Msa1396410:three_prime_UTR 45.0%
CGAATCAGCTACTGAGCTAT+TGG + tig0022022:76465-76484 None:intergenic 45.0%
GGTCAGATCTGAAAGGATTC+CGG + tig0022022:76493-76512 None:intergenic 45.0%
GTTTCATTCACCCGTTGGAT+CGG - tig0022022:76541-76560 Msa1396410:three_prime_UTR 45.0%
GCGAGACAAAGGAAGTGAAA+TGG + tig0022022:76587-76606 None:intergenic 45.0%
CGAAACAACAAAAGGGGGTA+AGG + tig0022022:76639-76658 None:intergenic 45.0%
!! GATCAAAAGTGTCGGTGCTA+TGG + tig0022022:76937-76956 None:intergenic 45.0%
AACCAAACACAGAGTGACAC+TGG + tig0022022:77048-77067 None:intergenic 45.0%
GTCACTCTGTGTTTGGTTGA+TGG - tig0022022:77050-77069 Msa1396410:CDS 45.0%
AATGAACTCAAGGGCTACCT+CGG + tig0022022:77173-77192 None:intergenic 45.0%
AGCGTAGTTCGTATAGGTTG+TGG + tig0022022:77273-77292 None:intergenic 45.0%
!! GTTTTTGTTTCCGGTCATCG+AGG - tig0022022:77532-77551 Msa1396410:five_prime_UTR 45.0%
!! TCATCGAGGTCTTGTTGGTT+CGG - tig0022022:77546-77565 Msa1396410:five_prime_UTR 45.0%
TTGAGTCAGCTTGCGAACAA+TGG + tig0022022:77572-77591 None:intergenic 45.0%
AATGACAAACTCAGGCTTCG+TGG + tig0022022:77677-77696 None:intergenic 45.0%
GCAGCTACAATGACAAACTC+AGG + tig0022022:77685-77704 None:intergenic 45.0%
ATTGTAGCTGCTGCCAAAGT+TGG - tig0022022:77694-77713 Msa1396410:intron 45.0%
! ATCAGCAGGATAGGTGTTGT+TGG + tig0022022:77731-77750 None:intergenic 45.0%
GGCAATGAAATCAGCAGGAT+AGG + tig0022022:77740-77759 None:intergenic 45.0%
! CAATGACATTGGTCTGGATC+TGG + tig0022022:77771-77790 None:intergenic 45.0%
! AGCAAAGCATCTTCAGGGAT+TGG + tig0022022:77871-77890 None:intergenic 45.0%
! CCAGTAAGCAAAGCATCTTC+AGG + tig0022022:77877-77896 None:intergenic 45.0%
CCTGAAGATGCTTTGCTTAC+TGG - tig0022022:77874-77893 Msa1396410:intron 45.0%
CCATTCATTTGTGGGCTCTA+AGG + tig0022022:77902-77921 None:intergenic 45.0%
CCTTAGAGCCCACAAATGAA+TGG - tig0022022:77899-77918 Msa1396410:intron 45.0%
TATGCCATTGCCAAGATTGC+CGG - tig0022022:77922-77941 Msa1396410:intron 45.0%
!! CATTTTGATCCCGGCAATCT+TGG + tig0022022:77935-77954 None:intergenic 45.0%
CACAAGTGGGATGCAATTTC+AGG - tig0022022:77976-77995 Msa1396410:intron 45.0%
! ACGTTTTGCCTGCTTTGATG+AGG - tig0022022:78052-78071 Msa1396410:intron 45.0%
! TTTTGCCTGCTTTGATGAGG+AGG - tig0022022:78055-78074 Msa1396410:intron 45.0%
TGAAACCTCCTCATCAAAGC+AGG + tig0022022:78063-78082 None:intergenic 45.0%
ACGTGCAAAAACTCCCTAAG+TGG + tig0022022:78138-78157 None:intergenic 45.0%
GAGCATTTGAATGTAGGGAG+TGG - tig0022022:78210-78229 Msa1396410:intron 45.0%
GGAATTGGCTGAGTCAATGA+AGG - tig0022022:78251-78270 Msa1396410:intron 45.0%
!!! GTTTTGAAGGCGATCTCGTT+TGG - tig0022022:78283-78302 Msa1396410:intron 45.0%
GAGCTATCCATCAGCTTTCT+TGG + tig0022022:78333-78352 None:intergenic 45.0%
GACACCGAAAGTCTCACTTA+AGG - tig0022022:78374-78393 Msa1396410:five_prime_UTR 45.0%
CCGAAAGTCTCACTTAAGGA+TGG - tig0022022:78378-78397 Msa1396410:five_prime_UTR 45.0%
TATTGGAGCGAGTCTGAGTT+GGG - tig0022022:78743-78762 Msa1396410:five_prime_UTR 45.0%
ACAGGAGGAAAACGTTGTGT+TGG - tig0022022:78823-78842 Msa1396410:five_prime_UTR 45.0%
CTATGCCTGCTTGCTTGTGT+AGG + tig0022022:76314-76333 None:intergenic 50.0%
CTACACAAGCAAGCAGGCAT+AGG - tig0022022:76312-76331 Msa1396410:three_prime_UTR 50.0%
!! CAGTAGCTGATTCGATCTGC+CGG - tig0022022:76471-76490 Msa1396410:three_prime_UTR 50.0%
GGTGGAAGGTCAGATCTGAA+AGG + tig0022022:76500-76519 None:intergenic 50.0%
CCAACGGGTGAATGAAACTC+CGG + tig0022022:76539-76558 None:intergenic 50.0%
TGGCACTAACACACTGTCAC+CGG + tig0022022:77028-77047 None:intergenic 50.0%
! TGCCAGTGTCACTCTGTGTT+TGG - tig0022022:77043-77062 Msa1396410:CDS 50.0%
ACACAATGTCTCACTCTCCG+AGG - tig0022022:77153-77172 Msa1396410:CDS 50.0%
ACCAACAAGACCTCGATGAC+CGG + tig0022022:77545-77564 None:intergenic 50.0%
! GTTCGCAAGCTGACTCAACT+CGG - tig0022022:77574-77593 Msa1396410:five_prime_UTR 50.0%
! TTCGCAAGCTGACTCAACTC+GGG - tig0022022:77575-77594 Msa1396410:five_prime_UTR 50.0%
AGGCTTCGTGGATGCAAAGA+AGG + tig0022022:77665-77684 None:intergenic 50.0%
GGCATGGATACCACCAACTT+TGG + tig0022022:77710-77729 None:intergenic 50.0%
!! CAGGATAGGTGTTGTTGGCA+TGG + tig0022022:77726-77745 None:intergenic 50.0%
! TGACATTGGTCTGGATCTGG+AGG + tig0022022:77768-77787 None:intergenic 50.0%
!! GCATCTTCAGGGATTGGTTG+AGG + tig0022022:77865-77884 None:intergenic 50.0%
ATGCCATTGCCAAGATTGCC+GGG - tig0022022:77923-77942 Msa1396410:intron 50.0%
GGGATGCCGACCAACTTATA+CGG - tig0022022:77997-78016 Msa1396410:intron 50.0%
GAGGAGGTTTCATGAGGCAA+AGG - tig0022022:78071-78090 Msa1396410:intron 50.0%
AAAGGTCAACGGTGCAAAGG+AGG - tig0022022:78089-78108 Msa1396410:intron 50.0%
! TGCAAAGGAGGTAGTGGTGT+GGG - tig0022022:78101-78120 Msa1396410:intron 50.0%
GAATGTAGGGAGTGGAAAGG+AGG - tig0022022:78218-78237 Msa1396410:intron 50.0%
TGGGATAGTACTAAGCCCGA+TGG - tig0022022:78303-78322 Msa1396410:intron 50.0%
GGGATAGTACTAAGCCCGAT+GGG - tig0022022:78304-78323 Msa1396410:intron 50.0%
!! AGCTTTCTTGGTGTCCCATC+GGG + tig0022022:78321-78340 None:intergenic 50.0%
!! CAGCTTTCTTGGTGTCCCAT+CGG + tig0022022:78322-78341 None:intergenic 50.0%
TGGGACACCAAGAAAGCTGA+TGG - tig0022022:78323-78342 Msa1396410:intron 50.0%
AGCTCAAAACTTGCAGCCCT+TGG - tig0022022:78348-78367 Msa1396410:intron 50.0%
CAAAACTTGCAGCCCTTGGT+TGG - tig0022022:78352-78371 Msa1396410:five_prime_UTR 50.0%
!! GACTTTCGGTGTCCAACCAA+GGG + tig0022022:78367-78386 None:intergenic 50.0%
!! AGACTTTCGGTGTCCAACCA+AGG + tig0022022:78368-78387 None:intergenic 50.0%
GTATTGGAGCGAGTCTGAGT+TGG - tig0022022:78742-78761 Msa1396410:five_prime_UTR 50.0%
ATTGGAGCGAGTCTGAGTTG+GGG - tig0022022:78744-78763 Msa1396410:five_prime_UTR 50.0%
GGAATTCTGCTGCCACAGTT+TGG - tig0022022:78874-78893 Msa1396410:five_prime_UTR 50.0%
CAGATCTGACCTTCCACCAC+CGG - tig0022022:76502-76521 Msa1396410:three_prime_UTR 55.0%
AACTCCGGTGAATCGGTCTC+CGG + tig0022022:76524-76543 None:intergenic 55.0%
CCGGAGTTTCATTCACCCGT+TGG - tig0022022:76536-76555 Msa1396410:three_prime_UTR 55.0%
! TCCGGTCATCGAGGTCTTGT+TGG - tig0022022:77541-77560 Msa1396410:five_prime_UTR 55.0%
GTAGCTGCTGCCAAAGTTGG+TGG - tig0022022:77697-77716 Msa1396410:intron 55.0%
GGTCTGGATCTGGAGGTTGA+TGG + tig0022022:77761-77780 None:intergenic 55.0%
GATCCCGGCAATCTTGGCAA+TGG + tig0022022:77929-77948 None:intergenic 55.0%
GGCAAAGGTCAACGGTGCAA+AGG - tig0022022:78086-78105 Msa1396410:intron 55.0%
CAACGGTGCAAAGGAGGTAG+TGG - tig0022022:78095-78114 Msa1396410:intron 55.0%
! GTGCAAAGGAGGTAGTGGTG+TGG - tig0022022:78100-78119 Msa1396410:intron 55.0%
! GCAAAGGAGGTAGTGGTGTG+GGG - tig0022022:78102-78121 Msa1396410:intron 55.0%
! AATCGAGGAGGGTGCAGTGC+AGG - tig0022022:76364-76383 Msa1396410:three_prime_UTR 60.0%
!! AATCGGTCTCCGGTGGTGGA+AGG + tig0022022:76514-76533 None:intergenic 60.0%
! ACCACCGGAGACCGATTCAC+CGG - tig0022022:76517-76536 Msa1396410:three_prime_UTR 60.0%
! GGAGGTAGTGGTGTGGGGTA+CGG - tig0022022:78107-78126 Msa1396410:intron 60.0%
! GAGGTAGTGGTGTGGGGTAC+GGG - tig0022022:78108-78127 Msa1396410:intron 60.0%
! AGGTAGTGGTGTGGGGTACG+GGG - tig0022022:78109-78128 Msa1396410:intron 60.0%
GGGTACGGGGAGTCCACTTA+GGG - tig0022022:78122-78141 Msa1396410:intron 60.0%
! CGAGTCTGAGTTGGGGCACA+AGG - tig0022022:78751-78770 Msa1396410:five_prime_UTR 60.0%
GGTGAATCGGTCTCCGGTGG+TGG + tig0022022:76518-76537 None:intergenic 65.0%
TCCGGTGAATCGGTCTCCGG+TGG + tig0022022:76521-76540 None:intergenic 65.0%
GGGGTACGGGGAGTCCACTT+AGG - tig0022022:78121-78140 Msa1396410:intron 65.0%
Chromosome Type Strat End Strand Name
tig0022022 gene 76279 78897 76279 ID=Msa1396410;Name=Msa1396410
tig0022022 mRNA 76279 78897 76279 ID=Msa1396410-mRNA-1;Parent=Msa1396410;Name=Msa1396410-mRNA-1;_AED=0.11;_eAED=0.11;_QI=890|0.5|0.66|1|1|1|3|451|164
tig0022022 exon 76279 77676 76279 ID=Msa1396410-mRNA-1:exon:15508;Parent=Msa1396410-mRNA-1
tig0022022 exon 78349 78446 78349 ID=Msa1396410-mRNA-1:exon:15507;Parent=Msa1396410-mRNA-1
tig0022022 exon 78558 78897 78558 ID=Msa1396410-mRNA-1:exon:15506;Parent=Msa1396410-mRNA-1
tig0022022 five_prime_UTR 78558 78897 78558 ID=Msa1396410-mRNA-1:five_prime_utr;Parent=Msa1396410-mRNA-1
tig0022022 five_prime_UTR 78349 78446 78349 ID=Msa1396410-mRNA-1:five_prime_utr;Parent=Msa1396410-mRNA-1
tig0022022 five_prime_UTR 77225 77676 77225 ID=Msa1396410-mRNA-1:five_prime_utr;Parent=Msa1396410-mRNA-1
tig0022022 CDS 76730 77224 76730 ID=Msa1396410-mRNA-1:cds;Parent=Msa1396410-mRNA-1
tig0022022 three_prime_UTR 76279 76729 76279 ID=Msa1396410-mRNA-1:three_prime_utr;Parent=Msa1396410-mRNA-1
Gene Sequence

>Msa1396410

ATGTGTCAAGCATACAGAATTCAGCACAAGTGGGATGCAATTTCAGGGATGCCGACCAACTTATACGGACCAAATGATAATTTCCATCCCGAAAATTCACACGTTTTGCCTGCTTTGATGAGGAGGTTTCATGAGGCAAAGGTCAACGGTGCAAAGGAGGTAGTGGTGTGGGGTACGGGGAGTCCACTTAGGGAGTTTTTGCACGTTGACGATTTGGCAGATGCAGTTGTCTTTATGATGGAAAAGTACAGTGGAGTTGAGCATTTGAATGTAGGGAGTGGAAAGGAGGTTACAATTAAGGAATTGGCTGAGTCAATGAAGGAAGTTGTGGGTTTTGAAGGCGATCTCGTTTGGGATAGTACTAAGCCCGATGGGACACCAAGAAAGCTGATGGATAGCTCAAAACTTGCAGCCCTTGGTTGGACACCGAAAGTCTCACTTAAGGATGGGCTTGTTGATACCTATAAGTGGTACTTAGAGAATGTCAAGCAATGA
Protein sequence

>Msa1396410

MCQAYRIQHKWDAISGMPTNLYGPNDNFHPENSHVLPALMRRFHEAKVNGAKEVVVWGTGSPLREFLHVDDLADAVVFMMEKYSGVEHLNVGSGKEVTIKELAESMKEVVGFEGDLVWDSTKPDGTPRKLMDSSKLAALGWTPKVSLKDGLVDTYKWYLENVKQ*