AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1166100

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1166100 MtrunA17_Chr7g0268991 100.000 164 0 0 1 164 157 320 5.40e-122 346
Msa1166100 MtrunA17_Chr2g0314601 25.767 163 110 4 1 161 169 322 4.43e-12 63.2
Msa1166100 MtrunA17_Chr1g0147841 27.879 165 104 5 1 161 167 320 5.77e-12 62.8
Msa1166100 MtrunA17_Chr8g0359401 26.380 163 109 4 1 161 169 322 7.87e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1166100 AT1G73250.1 85.714 161 23 0 2 162 160 320 3.61e-105 304
Msa1166100 AT1G17890.2 84.756 164 25 0 1 164 154 317 9.03e-105 303
Msa1166100 AT1G17890.3 84.756 164 25 0 1 164 154 317 9.03e-105 303
Msa1166100 AT1G17890.1 84.756 164 25 0 1 164 162 325 1.08e-104 303
Msa1166100 AT3G14790.2 28.659 164 104 6 1 161 172 325 3.59e-14 69.7
Msa1166100 AT3G14790.1 28.659 164 104 6 1 161 169 322 4.14e-14 69.3
Msa1166100 AT1G53500.1 26.994 163 108 4 1 161 171 324 5.04e-12 63.2
Msa1166100 AT1G78570.1 25.153 163 111 4 1 161 169 322 5.88e-12 63.2

Find 141 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGAAGATGCTTTGCTTAC+TGG 0.149882 7_4:-58101553 Msa1166100:five_prime_UTR
ATTGGTTGAGGTGCAAATTT+AGG 0.159427 7_4:+58101577 None:intergenic
GAGCTATCCATCAGCTTTCT+TGG 0.194906 7_4:+58101097 None:intergenic
CACAAGTGGGATGCAATTTC+AGG 0.203663 7_4:-58101451 Msa1166100:CDS
TTTGTAGCAATTCTCAAATA+TGG 0.237057 7_4:-58102666 Msa1166100:five_prime_UTR
AACAATAACCCTTACTGTTT+TGG 0.240454 7_4:+58102639 None:intergenic
CCATTCATTTGTGGGCTCTA+AGG 0.251483 7_4:+58101528 None:intergenic
ATTCTCTAAGTACCACTTAT+AGG 0.298342 7_4:+58101015 None:intergenic
GAGGTAGTGGTGTGGGGTAC+GGG 0.306076 7_4:-58101319 Msa1166100:CDS
AACCTATATAAAACTTGATA+TGG 0.311729 7_4:+58100957 None:intergenic
CCAGTAAGCAAAGCATCTTC+AGG 0.327068 7_4:+58101553 None:intergenic
TGGAAAGGAGGTTACAATTA+AGG 0.329079 7_4:-58101197 Msa1166100:CDS
GGAGGTTACAATTAAGGAAT+TGG 0.338695 7_4:-58101191 Msa1166100:CDS
AATGGATCATACAATGTAAC+TGG 0.346295 7_4:+58100892 None:intergenic
TATTGGAGCGAGTCTGAGTT+GGG 0.359537 7_4:-58100684 Msa1166100:three_prime_UTR
ATGTCAAGCAATGATTCTTC+TGG 0.362973 7_4:-58100994 Msa1166100:exon
GGGATGCCGACCAACTTATA+CGG 0.369504 7_4:-58101430 Msa1166100:CDS
TTGGTTGAGGTGCAAATTTA+GGG 0.370655 7_4:+58101578 None:intergenic
CATTCATTTGTGGGCTCTAA+GGG 0.394688 7_4:+58101529 None:intergenic
AAACTGCTAATGGGTGTTCA+TGG 0.397523 7_4:+58100774 None:intergenic
CAATGACATTGGTCTGGATC+TGG 0.398176 7_4:+58101659 None:intergenic
AGCTTTCTTGGTGTCCCATC+GGG 0.401801 7_4:+58101109 None:intergenic
AGGTAGTGGTGTGGGGTACG+GGG 0.403323 7_4:-58101318 Msa1166100:CDS
TGTTGGTCTATCATAGCTTC+TGG 0.405355 7_4:-58100587 Msa1166100:three_prime_UTR
AATAACTCATCTTAGTGAAA+TGG 0.407614 7_4:+58100931 None:intergenic
TAGCTTCTGGTAATTAAAGT+TGG 0.409180 7_4:-58100574 Msa1166100:three_prime_UTR
GGAGGTAGTGGTGTGGGGTA+CGG 0.416144 7_4:-58101320 Msa1166100:CDS
TTGTAGCAATTCTCAAATAT+GGG 0.416582 7_4:-58102665 Msa1166100:five_prime_UTR
TCGGGATGGAAATTATCATT+TGG 0.418888 7_4:+58101406 None:intergenic
GGTGAATCGGTCTCCGGTGG+TGG 0.423844 7_4:+58102913 None:intergenic
AGCTCAAAACTTGCAGCCCT+TGG 0.432660 7_4:-58101079 Msa1166100:CDS
GGTCAGATCTGAAAGGATTC+CGG 0.434451 7_4:+58102938 None:intergenic
CGAATCAGCTACTGAGCTAT+TGG 0.436756 7_4:+58102966 None:intergenic
TATGCCATTGCCAAGATTGC+CGG 0.438517 7_4:-58101505 Msa1166100:five_prime_UTR
CAGCTTTCTTGGTGTCCCAT+CGG 0.440823 7_4:+58101108 None:intergenic
TTATCTTGTTCAAGATATCA+TGG 0.441075 7_4:-58100820 Msa1166100:three_prime_UTR
AGGAAGTGAAATGGGTTTGT+GGG 0.447678 7_4:+58102853 None:intergenic
TCTTCTACCGTTTAATTGAC+AGG 0.449371 7_4:-58100622 Msa1166100:three_prime_UTR
GCGAGACAAAGGAAGTGAAA+TGG 0.457266 7_4:+58102844 None:intergenic
GGAGTTGAGCATTTGAATGT+AGG 0.457314 7_4:-58101223 Msa1166100:CDS
GGGTACGGGGAGTCCACTTA+GGG 0.460892 7_4:-58101305 Msa1166100:CDS
TGAATGAAACTCCGGTGAAT+CGG 0.462794 7_4:+58102900 None:intergenic
TGCAAAGGAGGTAGTGGTGT+GGG 0.465275 7_4:-58101326 Msa1166100:CDS
TCATCGAGGTCTTGTTGGTT+CGG 0.467475 7_4:-58101881 Msa1166100:five_prime_UTR
AATCGAGGAGGGTGCAGTGC+AGG 0.470403 7_4:-58103064 Msa1166100:five_prime_UTR
ACCAACAAGACCTCGATGAC+CGG 0.471640 7_4:+58101885 None:intergenic
GGCATGGATACCACCAACTT+TGG 0.472668 7_4:+58101720 None:intergenic
AGCAAAGCATCTTCAGGGAT+TGG 0.474197 7_4:+58101559 None:intergenic
GGGGTACGGGGAGTCCACTT+AGG 0.476642 7_4:-58101306 Msa1166100:CDS
CAGAATCAATGACATTGGTC+TGG 0.477646 7_4:+58101653 None:intergenic
AGGCTTCGTGGATGCAAAGA+AGG 0.478791 7_4:+58101765 None:intergenic
GGAATTCTGCTGCCACAGTT+TGG 0.479103 7_4:-58100553 Msa1166100:three_prime_UTR
CCGGAGTTTCATTCACCCGT+TGG 0.480531 7_4:-58102892 Msa1166100:five_prime_UTR
GAGTTGAGCATTTGAATGTA+GGG 0.483402 7_4:-58101222 Msa1166100:CDS
ATCATTTGGTCCGTATAAGT+TGG 0.484602 7_4:+58101420 None:intergenic
ATGGCATACCATTCATTTGT+GGG 0.486092 7_4:+58101520 None:intergenic
TATGGGAAGCCAAAACAGTA+AGG 0.486587 7_4:-58102648 Msa1166100:five_prime_UTR
CAAAACTTGCAGCCCTTGGT+TGG 0.488475 7_4:-58101075 Msa1166100:CDS
GTTTCATTCACCCGTTGGAT+CGG 0.490068 7_4:-58102887 Msa1166100:five_prime_UTR
AAGGAAGTGAAATGGGTTTG+TGG 0.491120 7_4:+58102852 None:intergenic
GTATTGGAGCGAGTCTGAGT+TGG 0.491249 7_4:-58100685 Msa1166100:three_prime_UTR
CAGATCTGACCTTCCACCAC+CGG 0.492159 7_4:-58102926 Msa1166100:five_prime_UTR
CGAGACAAAGGAAGTGAAAT+GGG 0.493690 7_4:+58102845 None:intergenic
ATCAGCAGGATAGGTGTTGT+TGG 0.496759 7_4:+58101699 None:intergenic
AATGACAAACTCAGGCTTCG+TGG 0.498096 7_4:+58101753 None:intergenic
GCATCTTCAGGGATTGGTTG+AGG 0.503925 7_4:+58101565 None:intergenic
GTGTTCATGGTCTTTAACAA+TGG 0.505731 7_4:+58100787 None:intergenic
ATGCCATTGCCAAGATTGCC+GGG 0.506721 7_4:-58101504 Msa1166100:five_prime_UTR
GAATGTAGGGAGTGGAAAGG+AGG 0.511105 7_4:-58101209 Msa1166100:CDS
CCATCCTTAAGTGAGACTTT+CGG 0.511365 7_4:+58101049 None:intergenic
GTTCGCAAGCTGACTCAACT+CGG 0.514389 7_4:-58101853 Msa1166100:five_prime_UTR
AAAAGAAAATCCGATCCAAC+GGG 0.518860 7_4:+58102877 None:intergenic
CGAGTCTGAGTTGGGGCACA+AGG 0.519988 7_4:-58100676 Msa1166100:three_prime_UTR
CTATGCCTGCTTGCTTGTGT+AGG 0.523923 7_4:+58103117 None:intergenic
CAGGATAGGTGTTGTTGGCA+TGG 0.526132 7_4:+58101704 None:intergenic
ACAAGTGGGATGCAATTTCA+GGG 0.528356 7_4:-58101450 Msa1166100:CDS
CCTTAGAGCCCACAAATGAA+TGG 0.530586 7_4:-58101528 Msa1166100:five_prime_UTR
ATTGTAGCTGCTGCCAAAGT+TGG 0.538288 7_4:-58101733 Msa1166100:five_prime_UTR
GATCCCGGCAATCTTGGCAA+TGG 0.538775 7_4:+58101501 None:intergenic
GGTCTGGATCTGGAGGTTGA+TGG 0.540673 7_4:+58101669 None:intergenic
AATCGGTCTCCGGTGGTGGA+AGG 0.542203 7_4:+58102917 None:intergenic
CCGAAAGTCTCACTTAAGGA+TGG 0.543510 7_4:-58101049 Msa1166100:CDS
TGAAACCTCCTCATCAAAGC+AGG 0.544927 7_4:+58101367 None:intergenic
CAACGGTGCAAAGGAGGTAG+TGG 0.546785 7_4:-58101332 Msa1166100:CDS
CAGTAAGCAAAGCATCTTCA+GGG 0.549758 7_4:+58101554 None:intergenic
CAGTAGCTGATTCGATCTGC+CGG 0.550955 7_4:-58102957 Msa1166100:five_prime_UTR
AACTCCGGTGAATCGGTCTC+CGG 0.553123 7_4:+58102907 None:intergenic
ACCACCGGAGACCGATTCAC+CGG 0.555662 7_4:-58102911 Msa1166100:five_prime_UTR
GAGTCAATGAAGGAAGTTGT+GGG 0.556089 7_4:-58101166 Msa1166100:CDS
TTGATGGCAATGAAATCAGC+AGG 0.556836 7_4:+58101685 None:intergenic
GGAATTGGCTGAGTCAATGA+AGG 0.558355 7_4:-58101176 Msa1166100:CDS
TTCGCAAGCTGACTCAACTC+GGG 0.561081 7_4:-58101852 Msa1166100:five_prime_UTR
AATGGCATACCATTCATTTG+TGG 0.562972 7_4:+58101519 None:intergenic
AGATGCAGTTGTCTTTATGA+TGG 0.567147 7_4:-58101257 Msa1166100:CDS
GACACCGAAAGTCTCACTTA+AGG 0.568664 7_4:-58101053 Msa1166100:CDS
GGCAATGAAATCAGCAGGAT+AGG 0.569157 7_4:+58101690 None:intergenic
TGGGACACCAAGAAAGCTGA+TGG 0.571175 7_4:-58101104 Msa1166100:CDS
GAGCATTTGAATGTAGGGAG+TGG 0.573638 7_4:-58101217 Msa1166100:CDS
CTACACAAGCAAGCAGGCAT+AGG 0.576067 7_4:-58103116 Msa1166100:five_prime_UTR
ACAGGAGGAAAACGTTGTGT+TGG 0.579417 7_4:-58100604 Msa1166100:three_prime_UTR
CAAATGACACAAGAATAACT+TGG 0.579888 7_4:+58100733 None:intergenic
GTAGCTGCTGCCAAAGTTGG+TGG 0.582192 7_4:-58101730 Msa1166100:five_prime_UTR
ATTGATTCTGCTTATCGCAA+TGG 0.583179 7_4:-58101640 Msa1166100:five_prime_UTR
GAGGAGGTTTCATGAGGCAA+AGG 0.584336 7_4:-58101356 Msa1166100:CDS
TTGAGTCAGCTTGCGAACAA+TGG 0.584587 7_4:+58101858 None:intergenic
TAAAAGAAAATCCGATCCAA+CGG 0.586666 7_4:+58102876 None:intergenic
CATACAGAATTCAGCACAAG+TGG 0.592816 7_4:-58101465 Msa1166100:CDS
TCCGGTGAATCGGTCTCCGG+TGG 0.593507 7_4:+58102910 None:intergenic
CGAAAGTCTCACTTAAGGAT+GGG 0.593671 7_4:-58101048 Msa1166100:CDS
GCAGCTACAATGACAAACTC+AGG 0.596147 7_4:+58101745 None:intergenic
GCAAAGGAGGTAGTGGTGTG+GGG 0.596327 7_4:-58101325 Msa1166100:CDS
TTTGGTCCGTATAAGTTGGT+CGG 0.596979 7_4:+58101424 None:intergenic
AATTTCCTACACAAGCAAGC+AGG 0.598067 7_4:-58103122 Msa1166100:five_prime_UTR
GACTTTCGGTGTCCAACCAA+GGG 0.600503 7_4:+58101063 None:intergenic
GGCAAAGGTCAACGGTGCAA+AGG 0.605297 7_4:-58101341 Msa1166100:CDS
TTTCATGAGGCAAAGGTCAA+CGG 0.606919 7_4:-58101349 Msa1166100:CDS
AGACTTTCGGTGTCCAACCA+AGG 0.607421 7_4:+58101062 None:intergenic
TAAAACTTGAAGCGAGACAA+AGG 0.607508 7_4:+58102833 None:intergenic
ATAAGCAGAATCAATGACAT+TGG 0.607720 7_4:+58101648 None:intergenic
GTGCAAAGGAGGTAGTGGTG+TGG 0.615768 7_4:-58101327 Msa1166100:CDS
ATGACACAAGAATAACTTGG+TGG 0.616110 7_4:+58100736 None:intergenic
GGCTTGTTGATACCTATAAG+TGG 0.616689 7_4:-58101027 Msa1166100:CDS
GGAGCACAACAACAGTACAT+AGG 0.619172 7_4:-58103095 Msa1166100:five_prime_UTR
TTTGAATGTAGGGAGTGGAA+AGG 0.619261 7_4:-58101212 Msa1166100:CDS
AAAGGTCAACGGTGCAAAGG+AGG 0.620001 7_4:-58101338 Msa1166100:CDS
ATGGGAAGCCAAAACAGTAA+GGG 0.622809 7_4:-58102647 Msa1166100:five_prime_UTR
TCTAATCTTAAGAACTCATG+CGG 0.625073 7_4:-58101821 Msa1166100:five_prime_UTR
ATGAGATGAGAGAATGAGTG+AGG 0.634514 7_4:+58102995 None:intergenic
TTTGATGAGGAGGTTTCATG+AGG 0.636802 7_4:-58101362 Msa1166100:CDS
CCAACGGGTGAATGAAACTC+CGG 0.651535 7_4:+58102892 None:intergenic
AAATTTAGGGTAAATGCAAG+AGG 0.655370 7_4:+58101591 None:intergenic
TGACATTGGTCTGGATCTGG+AGG 0.655686 7_4:+58101662 None:intergenic
TCTACCGTTTAATTGACAGG+AGG 0.655863 7_4:-58100619 Msa1166100:three_prime_UTR
TGAGTCAATGAAGGAAGTTG+TGG 0.655904 7_4:-58101167 Msa1166100:CDS
GGTGGAAGGTCAGATCTGAA+AGG 0.658870 7_4:+58102931 None:intergenic
GATGAAGCAACACAAGAGAA+CGG 0.659980 7_4:+58103022 None:intergenic
ATTCATTTGTGGGCTCTAAG+GGG 0.661471 7_4:+58101530 None:intergenic
ATTGGAGCGAGTCTGAGTTG+GGG 0.680747 7_4:-58100683 Msa1166100:three_prime_UTR
TTTATGATGGAAAAGTACAG+TGG 0.685676 7_4:-58101244 Msa1166100:CDS
TGACTCAAGCTACCAAACTG+TGG 0.689923 7_4:+58100541 None:intergenic
ATACAGAATTCAGCACAAGT+GGG 0.695878 7_4:-58101464 Msa1166100:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTTTTGTTAAATTTTGAAA+TGG - chr7_4:58100947-58100966 Msa1166100:three_prime_UTR 15.0%
!!! TGATTTTTTTTTTTTTTTGC+AGG - chr7_4:58100981-58101000 Msa1166100:three_prime_UTR 15.0%
!!! TTTTTTTGCAGGATTTTATT+TGG - chr7_4:58100992-58101011 Msa1166100:exon 20.0%
!!! TTTATTTGGAATTTTGAAGT+TGG - chr7_4:58101006-58101025 Msa1166100:CDS 20.0%
!!! TTATTTTCTCAAATTACTAC+CGG - chr7_4:58101279-58101298 Msa1166100:CDS 20.0%
!! AACCTATATAAAACTTGATA+TGG + chr7_4:58102747-58102766 None:intergenic 20.0%
!!! ATGAGTTATTTTCTTTGTAT+TGG - chr7_4:58103000-58103019 Msa1166100:five_prime_UTR 20.0%
!! GAAAAAAAAAAATGCTGTAT+TGG - chr7_4:58103047-58103066 Msa1166100:five_prime_UTR 20.0%
! AAAATGACGAAACAACAAAA+GGG + chr7_4:58100919-58100938 None:intergenic 25.0%
! TAAAATGACGAAACAACAAA+AGG + chr7_4:58100920-58100939 None:intergenic 25.0%
! TTTGTAGCAATTCTCAAATA+TGG - chr7_4:58101035-58101054 Msa1166100:CDS 25.0%
! TTGTAGCAATTCTCAAATAT+GGG - chr7_4:58101036-58101055 Msa1166100:CDS 25.0%
! AACATTTAATGTGATCACAT+TGG + chr7_4:58101261-58101280 None:intergenic 25.0%
!!! CATTTGCTTTTGGTTTAATA+TGG - chr7_4:58101462-58101481 Msa1166100:CDS 25.0%
! TGGTTTAATATGGTATCAAT+AGG - chr7_4:58101472-58101491 Msa1166100:CDS 25.0%
!! CAGAATTTTACTAGATTCAA+AGG - chr7_4:58101585-58101604 Msa1166100:five_prime_UTR 25.0%
!! AATTTTACTAGATTCAAAGG+TGG - chr7_4:58101588-58101607 Msa1166100:five_prime_UTR 25.0%
! ATAACAATGCTAACTCATTA+TGG - chr7_4:58101644-58101663 Msa1166100:five_prime_UTR 25.0%
!!! GGTGAGAGAATTATTTTTAT+TGG - chr7_4:58101698-58101717 Msa1166100:five_prime_UTR 25.0%
!!! TGCCATATCAAGTTTTATAT+AGG - chr7_4:58102742-58102761 Msa1166100:intron 25.0%
! AATAACTCATCTTAGTGAAA+TGG + chr7_4:58102773-58102792 None:intergenic 25.0%
! ACGTAATTAGTACAGAAAAA+TGG + chr7_4:58102830-58102849 None:intergenic 25.0%
!! ATTTTTCTGTACTAATTACG+TGG - chr7_4:58102829-58102848 Msa1166100:intron 25.0%
!! TTTTTCTGTACTAATTACGT+GGG - chr7_4:58102830-58102849 Msa1166100:intron 25.0%
! TTATCTTGTTCAAGATATCA+TGG - chr7_4:58102881-58102900 Msa1166100:five_prime_UTR 25.0%
! ACACTAAAAAAACTGCTAAT+GGG + chr7_4:58102939-58102958 None:intergenic 25.0%
! AACACTAAAAAAACTGCTAA+TGG + chr7_4:58102940-58102959 None:intergenic 25.0%
ACATAGGTAAAAAACAATCG+AGG - chr7_4:58100622-58100641 Msa1166100:three_prime_UTR 30.0%
TAAAAGAAAATCCGATCCAA+CGG + chr7_4:58100828-58100847 None:intergenic 30.0%
AAATGACGAAACAACAAAAG+GGG + chr7_4:58100918-58100937 None:intergenic 30.0%
!! TTGTTTCGTCATTTTAGAAG+TGG - chr7_4:58100924-58100943 Msa1166100:three_prime_UTR 30.0%
!! TGTTTCGTCATTTTAGAAGT+GGG - chr7_4:58100925-58100944 Msa1166100:three_prime_UTR 30.0%
! AACAATAACCCTTACTGTTT+TGG + chr7_4:58101065-58101084 None:intergenic 30.0%
! ATGTCTTTGATCAAAAGTGT+CGG + chr7_4:58101218-58101237 None:intergenic 30.0%
!! TTAGCATTGTTATTGACTCT+TGG + chr7_4:58101637-58101656 None:intergenic 30.0%
!! TTTTGATGATAAATCTGCCA+AGG - chr7_4:58101784-58101803 Msa1166100:five_prime_UTR 30.0%
TCTAATCTTAAGAACTCATG+CGG - chr7_4:58101880-58101899 Msa1166100:five_prime_UTR 30.0%
ATAAGCAGAATCAATGACAT+TGG + chr7_4:58102056-58102075 None:intergenic 30.0%
!!! TGCTAAGAAGCTTTTGTTTT+TGG - chr7_4:58102084-58102103 Msa1166100:intron 30.0%
!!! GCTAAGAAGCTTTTGTTTTT+GGG - chr7_4:58102085-58102104 Msa1166100:intron 30.0%
! AAATTTAGGGTAAATGCAAG+AGG + chr7_4:58102113-58102132 None:intergenic 30.0%
! TTTATGATGGAAAAGTACAG+TGG - chr7_4:58102457-58102476 Msa1166100:intron 30.0%
ATTCTCTAAGTACCACTTAT+AGG + chr7_4:58102689-58102708 None:intergenic 30.0%
AATGGATCATACAATGTAAC+TGG + chr7_4:58102812-58102831 None:intergenic 30.0%
CAAATGACACAAGAATAACT+TGG + chr7_4:58102971-58102990 None:intergenic 30.0%
! TTTTCCTCCTGTCAATTAAA+CGG + chr7_4:58103089-58103108 None:intergenic 30.0%
TAGCTTCTGGTAATTAAAGT+TGG - chr7_4:58103127-58103146 Msa1166100:five_prime_UTR 30.0%
TAGGTAAAAAACAATCGAGG+AGG - chr7_4:58100625-58100644 Msa1166100:three_prime_UTR 35.0%
AGGTAAAAAACAATCGAGGA+GGG - chr7_4:58100626-58100645 Msa1166100:three_prime_UTR 35.0%
!! AGAGAACGGATTTTATGGAA+GGG + chr7_4:58100668-58100687 None:intergenic 35.0%
!! AAGAGAACGGATTTTATGGA+AGG + chr7_4:58100669-58100688 None:intergenic 35.0%
!! ACACAAGAGAACGGATTTTA+TGG + chr7_4:58100673-58100692 None:intergenic 35.0%
AAAAGAAAATCCGATCCAAC+GGG + chr7_4:58100827-58100846 None:intergenic 35.0%
TAAAACTTGAAGCGAGACAA+AGG + chr7_4:58100871-58100890 None:intergenic 35.0%
!! TTTGTCTCGCTTCAAGTTTT+AGG - chr7_4:58100870-58100889 Msa1166100:three_prime_UTR 35.0%
AATGACGAAACAACAAAAGG+GGG + chr7_4:58100917-58100936 None:intergenic 35.0%
TCGAAAAAAACGCTCAATTG+CGG + chr7_4:58101394-58101413 None:intergenic 35.0%
CCAAAAGCAAATGAACTCAA+GGG + chr7_4:58101455-58101474 None:intergenic 35.0%
ACCAAAAGCAAATGAACTCA+AGG + chr7_4:58101456-58101475 None:intergenic 35.0%
!!! TAGCTGTTTTTCCAGTTTGT+AGG - chr7_4:58101497-58101516 Msa1166100:exon 35.0%
TATAATCAGGACCTACAAAC+TGG + chr7_4:58101511-58101530 None:intergenic 35.0%
GTCAACGAGAACTTATAATC+AGG + chr7_4:58101524-58101543 None:intergenic 35.0%
AAAGATAGCGTAGTTCGTAT+AGG + chr7_4:58101552-58101571 None:intergenic 35.0%
TTGCCCTAAAAAAAGAAGAG+AGG - chr7_4:58101676-58101695 Msa1166100:five_prime_UTR 35.0%
TGCCCTAAAAAAAGAAGAGA+GGG - chr7_4:58101677-58101696 Msa1166100:five_prime_UTR 35.0%
!!! CACCCTCTCTTCTTTTTTTA+GGG + chr7_4:58101682-58101701 None:intergenic 35.0%
!!! TCACCCTCTCTTCTTTTTTT+AGG + chr7_4:58101683-58101702 None:intergenic 35.0%
! ATTGATTCTGCTTATCGCAA+TGG - chr7_4:58102061-58102080 Msa1166100:intron 35.0%
TTGGTTGAGGTGCAAATTTA+GGG + chr7_4:58102126-58102145 None:intergenic 35.0%
ATTGGTTGAGGTGCAAATTT+AGG + chr7_4:58102127-58102146 None:intergenic 35.0%
ATGGCATACCATTCATTTGT+GGG + chr7_4:58102184-58102203 None:intergenic 35.0%
AATGGCATACCATTCATTTG+TGG + chr7_4:58102185-58102204 None:intergenic 35.0%
ATACAGAATTCAGCACAAGT+GGG - chr7_4:58102237-58102256 Msa1166100:intron 35.0%
ATCATTTGGTCCGTATAAGT+TGG + chr7_4:58102284-58102303 None:intergenic 35.0%
TCGGGATGGAAATTATCATT+TGG + chr7_4:58102298-58102317 None:intergenic 35.0%
! AGGCAAAACGTGTGAATTTT+CGG + chr7_4:58102317-58102336 None:intergenic 35.0%
AGATGCAGTTGTCTTTATGA+TGG - chr7_4:58102444-58102463 Msa1166100:intron 35.0%
GAGTTGAGCATTTGAATGTA+GGG - chr7_4:58102479-58102498 Msa1166100:intron 35.0%
TGGAAAGGAGGTTACAATTA+AGG - chr7_4:58102504-58102523 Msa1166100:intron 35.0%
GGAGGTTACAATTAAGGAAT+TGG - chr7_4:58102510-58102529 Msa1166100:intron 35.0%
ATGTCAAGCAATGATTCTTC+TGG - chr7_4:58102707-58102726 Msa1166100:intron 35.0%
GTGTTCATGGTCTTTAACAA+TGG + chr7_4:58102917-58102936 None:intergenic 35.0%
ATGACACAAGAATAACTTGG+TGG + chr7_4:58102968-58102987 None:intergenic 35.0%
TCTTCTACCGTTTAATTGAC+AGG - chr7_4:58103079-58103098 Msa1166100:five_prime_UTR 35.0%
AATTTCCTACACAAGCAAGC+AGG - chr7_4:58100579-58100598 Msa1166100:three_prime_UTR 40.0%
GATGAAGCAACACAAGAGAA+CGG + chr7_4:58100682-58100701 None:intergenic 40.0%
ATGAGATGAGAGAATGAGTG+AGG + chr7_4:58100709-58100728 None:intergenic 40.0%
TGAATGAAACTCCGGTGAAT+CGG + chr7_4:58100804-58100823 None:intergenic 40.0%
! AGGAAGTGAAATGGGTTTGT+GGG + chr7_4:58100851-58100870 None:intergenic 40.0%
! AAGGAAGTGAAATGGGTTTG+TGG + chr7_4:58100852-58100871 None:intergenic 40.0%
CGAGACAAAGGAAGTGAAAT+GGG + chr7_4:58100859-58100878 None:intergenic 40.0%
TATGGGAAGCCAAAACAGTA+AGG - chr7_4:58101053-58101072 Msa1166100:CDS 40.0%
ATGGGAAGCCAAAACAGTAA+GGG - chr7_4:58101054-58101073 Msa1166100:CDS 40.0%
! CACATTGGTTGAAGTTGTGT+CGG + chr7_4:58101246-58101265 None:intergenic 40.0%
CTAAAGCAATCGCTTCTGAA+AGG - chr7_4:58101352-58101371 Msa1166100:CDS 40.0%
! CCCTTGAGTTCATTTGCTTT+TGG - chr7_4:58101452-58101471 Msa1166100:CDS 40.0%
!! TCTGCCAAGGTTTTTGTTTC+CGG - chr7_4:58101797-58101816 Msa1166100:five_prime_UTR 40.0%
ATGACCGGAAACAAAAACCT+TGG + chr7_4:58101804-58101823 None:intergenic 40.0%
! TTGATGGCAATGAAATCAGC+AGG + chr7_4:58102019-58102038 None:intergenic 40.0%
CAGAATCAATGACATTGGTC+TGG + chr7_4:58102051-58102070 None:intergenic 40.0%
! CAGTAAGCAAAGCATCTTCA+GGG + chr7_4:58102150-58102169 None:intergenic 40.0%
!! ATTCATTTGTGGGCTCTAAG+GGG + chr7_4:58102174-58102193 None:intergenic 40.0%
! CATTCATTTGTGGGCTCTAA+GGG + chr7_4:58102175-58102194 None:intergenic 40.0%
!! TGCTTGACACATTTTGATCC+CGG + chr7_4:58102218-58102237 None:intergenic 40.0%
CATACAGAATTCAGCACAAG+TGG - chr7_4:58102236-58102255 Msa1166100:intron 40.0%
ACAAGTGGGATGCAATTTCA+GGG - chr7_4:58102251-58102270 Msa1166100:intron 40.0%
!! TTTGGTCCGTATAAGTTGGT+CGG + chr7_4:58102280-58102299 None:intergenic 40.0%
!! AAACGTGTGAATTTTCGGGA+TGG + chr7_4:58102312-58102331 None:intergenic 40.0%
! GGCAAAACGTGTGAATTTTC+GGG + chr7_4:58102316-58102335 None:intergenic 40.0%
TTTGATGAGGAGGTTTCATG+AGG - chr7_4:58102339-58102358 Msa1166100:intron 40.0%
TTTCATGAGGCAAAGGTCAA+CGG - chr7_4:58102352-58102371 Msa1166100:intron 40.0%
! GTTTTTGCACGTTGACGATT+TGG - chr7_4:58102420-58102439 Msa1166100:intron 40.0%
GGAGTTGAGCATTTGAATGT+AGG - chr7_4:58102478-58102497 Msa1166100:intron 40.0%
TTTGAATGTAGGGAGTGGAA+AGG - chr7_4:58102489-58102508 Msa1166100:intron 40.0%
TGAGTCAATGAAGGAAGTTG+TGG - chr7_4:58102534-58102553 Msa1166100:intron 40.0%
GAGTCAATGAAGGAAGTTGT+GGG - chr7_4:58102535-58102554 Msa1166100:intron 40.0%
!! AAGGAAGTTGTGGGTTTTGA+AGG - chr7_4:58102544-58102563 Msa1166100:intron 40.0%
!! TTTTGAAGGCGATCTCGTTT+GGG - chr7_4:58102558-58102577 Msa1166100:intron 40.0%
CCATCCTTAAGTGAGACTTT+CGG + chr7_4:58102655-58102674 None:intergenic 40.0%
CGAAAGTCTCACTTAAGGAT+GGG - chr7_4:58102653-58102672 Msa1166100:five_prime_UTR 40.0%
!! GGCTTGTTGATACCTATAAG+TGG - chr7_4:58102674-58102693 Msa1166100:five_prime_UTR 40.0%
! AAACTGCTAATGGGTGTTCA+TGG + chr7_4:58102930-58102949 None:intergenic 40.0%
TCTACCGTTTAATTGACAGG+AGG - chr7_4:58103082-58103101 Msa1166100:five_prime_UTR 40.0%
! TGTTGGTCTATCATAGCTTC+TGG - chr7_4:58103114-58103133 Msa1166100:five_prime_UTR 40.0%
GGAGCACAACAACAGTACAT+AGG - chr7_4:58100606-58100625 Msa1166100:three_prime_UTR 45.0%
CGAATCAGCTACTGAGCTAT+TGG + chr7_4:58100738-58100757 None:intergenic 45.0%
GGTCAGATCTGAAAGGATTC+CGG + chr7_4:58100766-58100785 None:intergenic 45.0%
GTTTCATTCACCCGTTGGAT+CGG - chr7_4:58100814-58100833 Msa1166100:three_prime_UTR 45.0%
GCGAGACAAAGGAAGTGAAA+TGG + chr7_4:58100860-58100879 None:intergenic 45.0%
CGAAACAACAAAAGGGGGTA+AGG + chr7_4:58100912-58100931 None:intergenic 45.0%
!! GATCAAAAGTGTCGGTGCTA+TGG + chr7_4:58101210-58101229 None:intergenic 45.0%
AACCAAACACAGAGTGACAC+TGG + chr7_4:58101321-58101340 None:intergenic 45.0%
GTCACTCTGTGTTTGGTTGA+TGG - chr7_4:58101323-58101342 Msa1166100:CDS 45.0%
AATGAACTCAAGGGCTACCT+CGG + chr7_4:58101446-58101465 None:intergenic 45.0%
AGCGTAGTTCGTATAGGTTG+TGG + chr7_4:58101546-58101565 None:intergenic 45.0%
!! GTTTTTGTTTCCGGTCATCG+AGG - chr7_4:58101806-58101825 Msa1166100:five_prime_UTR 45.0%
!! TCATCGAGGTCTTGTTGGTT+CGG - chr7_4:58101820-58101839 Msa1166100:five_prime_UTR 45.0%
TTGAGTCAGCTTGCGAACAA+TGG + chr7_4:58101846-58101865 None:intergenic 45.0%
AATGACAAACTCAGGCTTCG+TGG + chr7_4:58101951-58101970 None:intergenic 45.0%
GCAGCTACAATGACAAACTC+AGG + chr7_4:58101959-58101978 None:intergenic 45.0%
ATTGTAGCTGCTGCCAAAGT+TGG - chr7_4:58101968-58101987 Msa1166100:intron 45.0%
! ATCAGCAGGATAGGTGTTGT+TGG + chr7_4:58102005-58102024 None:intergenic 45.0%
GGCAATGAAATCAGCAGGAT+AGG + chr7_4:58102014-58102033 None:intergenic 45.0%
! CAATGACATTGGTCTGGATC+TGG + chr7_4:58102045-58102064 None:intergenic 45.0%
! AGCAAAGCATCTTCAGGGAT+TGG + chr7_4:58102145-58102164 None:intergenic 45.0%
! CCAGTAAGCAAAGCATCTTC+AGG + chr7_4:58102151-58102170 None:intergenic 45.0%
CCTGAAGATGCTTTGCTTAC+TGG - chr7_4:58102148-58102167 Msa1166100:intron 45.0%
CCATTCATTTGTGGGCTCTA+AGG + chr7_4:58102176-58102195 None:intergenic 45.0%
CCTTAGAGCCCACAAATGAA+TGG - chr7_4:58102173-58102192 Msa1166100:intron 45.0%
TATGCCATTGCCAAGATTGC+CGG - chr7_4:58102196-58102215 Msa1166100:intron 45.0%
!! CATTTTGATCCCGGCAATCT+TGG + chr7_4:58102209-58102228 None:intergenic 45.0%
CACAAGTGGGATGCAATTTC+AGG - chr7_4:58102250-58102269 Msa1166100:intron 45.0%
! ACGTTTTGCCTGCTTTGATG+AGG - chr7_4:58102326-58102345 Msa1166100:intron 45.0%
! TTTTGCCTGCTTTGATGAGG+AGG - chr7_4:58102329-58102348 Msa1166100:intron 45.0%
TGAAACCTCCTCATCAAAGC+AGG + chr7_4:58102337-58102356 None:intergenic 45.0%
ACGTGCAAAAACTCCCTAAG+TGG + chr7_4:58102412-58102431 None:intergenic 45.0%
GAGCATTTGAATGTAGGGAG+TGG - chr7_4:58102484-58102503 Msa1166100:intron 45.0%
GGAATTGGCTGAGTCAATGA+AGG - chr7_4:58102525-58102544 Msa1166100:intron 45.0%
!!! GTTTTGAAGGCGATCTCGTT+TGG - chr7_4:58102557-58102576 Msa1166100:intron 45.0%
GAGCTATCCATCAGCTTTCT+TGG + chr7_4:58102607-58102626 None:intergenic 45.0%
GACACCGAAAGTCTCACTTA+AGG - chr7_4:58102648-58102667 Msa1166100:five_prime_UTR 45.0%
CCGAAAGTCTCACTTAAGGA+TGG - chr7_4:58102652-58102671 Msa1166100:five_prime_UTR 45.0%
TATTGGAGCGAGTCTGAGTT+GGG - chr7_4:58103017-58103036 Msa1166100:five_prime_UTR 45.0%
ACAGGAGGAAAACGTTGTGT+TGG - chr7_4:58103097-58103116 Msa1166100:five_prime_UTR 45.0%
CTATGCCTGCTTGCTTGTGT+AGG + chr7_4:58100587-58100606 None:intergenic 50.0%
CTACACAAGCAAGCAGGCAT+AGG - chr7_4:58100585-58100604 Msa1166100:three_prime_UTR 50.0%
!! CAGTAGCTGATTCGATCTGC+CGG - chr7_4:58100744-58100763 Msa1166100:three_prime_UTR 50.0%
GGTGGAAGGTCAGATCTGAA+AGG + chr7_4:58100773-58100792 None:intergenic 50.0%
CCAACGGGTGAATGAAACTC+CGG + chr7_4:58100812-58100831 None:intergenic 50.0%
TGGCACTAACACACTGTCAC+CGG + chr7_4:58101301-58101320 None:intergenic 50.0%
! TGCCAGTGTCACTCTGTGTT+TGG - chr7_4:58101316-58101335 Msa1166100:CDS 50.0%
ACACAATGTCTCACTCTCCG+AGG - chr7_4:58101426-58101445 Msa1166100:CDS 50.0%
ACCAACAAGACCTCGATGAC+CGG + chr7_4:58101819-58101838 None:intergenic 50.0%
! GTTCGCAAGCTGACTCAACT+CGG - chr7_4:58101848-58101867 Msa1166100:five_prime_UTR 50.0%
! TTCGCAAGCTGACTCAACTC+GGG - chr7_4:58101849-58101868 Msa1166100:five_prime_UTR 50.0%
AGGCTTCGTGGATGCAAAGA+AGG + chr7_4:58101939-58101958 None:intergenic 50.0%
GGCATGGATACCACCAACTT+TGG + chr7_4:58101984-58102003 None:intergenic 50.0%
!! CAGGATAGGTGTTGTTGGCA+TGG + chr7_4:58102000-58102019 None:intergenic 50.0%
! TGACATTGGTCTGGATCTGG+AGG + chr7_4:58102042-58102061 None:intergenic 50.0%
!! GCATCTTCAGGGATTGGTTG+AGG + chr7_4:58102139-58102158 None:intergenic 50.0%
ATGCCATTGCCAAGATTGCC+GGG - chr7_4:58102197-58102216 Msa1166100:intron 50.0%
GGGATGCCGACCAACTTATA+CGG - chr7_4:58102271-58102290 Msa1166100:intron 50.0%
GAGGAGGTTTCATGAGGCAA+AGG - chr7_4:58102345-58102364 Msa1166100:intron 50.0%
AAAGGTCAACGGTGCAAAGG+AGG - chr7_4:58102363-58102382 Msa1166100:intron 50.0%
! TGCAAAGGAGGTAGTGGTGT+GGG - chr7_4:58102375-58102394 Msa1166100:intron 50.0%
GAATGTAGGGAGTGGAAAGG+AGG - chr7_4:58102492-58102511 Msa1166100:intron 50.0%
TGGGATAGTACTAAGCCCGA+TGG - chr7_4:58102577-58102596 Msa1166100:intron 50.0%
GGGATAGTACTAAGCCCGAT+GGG - chr7_4:58102578-58102597 Msa1166100:intron 50.0%
!! AGCTTTCTTGGTGTCCCATC+GGG + chr7_4:58102595-58102614 None:intergenic 50.0%
!! CAGCTTTCTTGGTGTCCCAT+CGG + chr7_4:58102596-58102615 None:intergenic 50.0%
TGGGACACCAAGAAAGCTGA+TGG - chr7_4:58102597-58102616 Msa1166100:intron 50.0%
AGCTCAAAACTTGCAGCCCT+TGG - chr7_4:58102622-58102641 Msa1166100:intron 50.0%
CAAAACTTGCAGCCCTTGGT+TGG - chr7_4:58102626-58102645 Msa1166100:five_prime_UTR 50.0%
!! GACTTTCGGTGTCCAACCAA+GGG + chr7_4:58102641-58102660 None:intergenic 50.0%
!! AGACTTTCGGTGTCCAACCA+AGG + chr7_4:58102642-58102661 None:intergenic 50.0%
GTATTGGAGCGAGTCTGAGT+TGG - chr7_4:58103016-58103035 Msa1166100:five_prime_UTR 50.0%
ATTGGAGCGAGTCTGAGTTG+GGG - chr7_4:58103018-58103037 Msa1166100:five_prime_UTR 50.0%
GGAATTCTGCTGCCACAGTT+TGG - chr7_4:58103148-58103167 Msa1166100:five_prime_UTR 50.0%
CAGATCTGACCTTCCACCAC+CGG - chr7_4:58100775-58100794 Msa1166100:three_prime_UTR 55.0%
AACTCCGGTGAATCGGTCTC+CGG + chr7_4:58100797-58100816 None:intergenic 55.0%
CCGGAGTTTCATTCACCCGT+TGG - chr7_4:58100809-58100828 Msa1166100:three_prime_UTR 55.0%
! TCCGGTCATCGAGGTCTTGT+TGG - chr7_4:58101815-58101834 Msa1166100:five_prime_UTR 55.0%
GTAGCTGCTGCCAAAGTTGG+TGG - chr7_4:58101971-58101990 Msa1166100:intron 55.0%
GGTCTGGATCTGGAGGTTGA+TGG + chr7_4:58102035-58102054 None:intergenic 55.0%
GATCCCGGCAATCTTGGCAA+TGG + chr7_4:58102203-58102222 None:intergenic 55.0%
GGCAAAGGTCAACGGTGCAA+AGG - chr7_4:58102360-58102379 Msa1166100:intron 55.0%
CAACGGTGCAAAGGAGGTAG+TGG - chr7_4:58102369-58102388 Msa1166100:intron 55.0%
! GTGCAAAGGAGGTAGTGGTG+TGG - chr7_4:58102374-58102393 Msa1166100:intron 55.0%
! GCAAAGGAGGTAGTGGTGTG+GGG - chr7_4:58102376-58102395 Msa1166100:intron 55.0%
! AATCGAGGAGGGTGCAGTGC+AGG - chr7_4:58100637-58100656 Msa1166100:three_prime_UTR 60.0%
!! AATCGGTCTCCGGTGGTGGA+AGG + chr7_4:58100787-58100806 None:intergenic 60.0%
! ACCACCGGAGACCGATTCAC+CGG - chr7_4:58100790-58100809 Msa1166100:three_prime_UTR 60.0%
! GGAGGTAGTGGTGTGGGGTA+CGG - chr7_4:58102381-58102400 Msa1166100:intron 60.0%
! GAGGTAGTGGTGTGGGGTAC+GGG - chr7_4:58102382-58102401 Msa1166100:intron 60.0%
! AGGTAGTGGTGTGGGGTACG+GGG - chr7_4:58102383-58102402 Msa1166100:intron 60.0%
GGGTACGGGGAGTCCACTTA+GGG - chr7_4:58102396-58102415 Msa1166100:intron 60.0%
! CGAGTCTGAGTTGGGGCACA+AGG - chr7_4:58103025-58103044 Msa1166100:five_prime_UTR 60.0%
GGTGAATCGGTCTCCGGTGG+TGG + chr7_4:58100791-58100810 None:intergenic 65.0%
TCCGGTGAATCGGTCTCCGG+TGG + chr7_4:58100794-58100813 None:intergenic 65.0%
GGGGTACGGGGAGTCCACTT+AGG - chr7_4:58102395-58102414 Msa1166100:intron 65.0%
Chromosome Type Strat End Strand Name
chr7_4 gene 58100552 58103171 58100552 ID=Msa1166100;Name=Msa1166100
chr7_4 mRNA 58100552 58103171 58100552 ID=Msa1166100-mRNA-1;Parent=Msa1166100;Name=Msa1166100-mRNA-1;_AED=0.11;_eAED=0.11;_QI=890|0.5|0.66|1|1|1|3|451|164
chr7_4 exon 58100552 58101949 58100552 ID=Msa1166100-mRNA-1:exon:17776;Parent=Msa1166100-mRNA-1
chr7_4 exon 58102623 58102720 58102623 ID=Msa1166100-mRNA-1:exon:17775;Parent=Msa1166100-mRNA-1
chr7_4 exon 58102832 58103171 58102832 ID=Msa1166100-mRNA-1:exon:17774;Parent=Msa1166100-mRNA-1
chr7_4 five_prime_UTR 58102832 58103171 58102832 ID=Msa1166100-mRNA-1:five_prime_utr;Parent=Msa1166100-mRNA-1
chr7_4 five_prime_UTR 58102623 58102720 58102623 ID=Msa1166100-mRNA-1:five_prime_utr;Parent=Msa1166100-mRNA-1
chr7_4 five_prime_UTR 58101498 58101949 58101498 ID=Msa1166100-mRNA-1:five_prime_utr;Parent=Msa1166100-mRNA-1
chr7_4 CDS 58101003 58101497 58101003 ID=Msa1166100-mRNA-1:cds;Parent=Msa1166100-mRNA-1
chr7_4 three_prime_UTR 58100552 58101002 58100552 ID=Msa1166100-mRNA-1:three_prime_utr;Parent=Msa1166100-mRNA-1
Gene Sequence

>Msa1166100

ATGTGTCAAGCATACAGAATTCAGCACAAGTGGGATGCAATTTCAGGGATGCCGACCAACTTATACGGACCAAATGATAATTTCCATCCCGAAAATTCACACGTTTTGCCTGCTTTGATGAGGAGGTTTCATGAGGCAAAGGTCAACGGTGCAAAGGAGGTAGTGGTGTGGGGTACGGGGAGTCCACTTAGGGAGTTTTTGCACGTTGACGATTTGGCAGATGCAGTTGTCTTTATGATGGAAAAGTACAGTGGAGTTGAGCATTTGAATGTAGGGAGTGGAAAGGAGGTTACAATTAAGGAATTGGCTGAGTCAATGAAGGAAGTTGTGGGTTTTGAAGGCGATCTCGTTTGGGATAGTACTAAGCCCGATGGGACACCAAGAAAGCTGATGGATAGCTCAAAACTTGCAGCCCTTGGTTGGACACCGAAAGTCTCACTTAAGGATGGGCTTGTTGATACCTATAAGTGGTACTTAGAGAATGTCAAGCAATGA
Protein sequence

>Msa1166100

MCQAYRIQHKWDAISGMPTNLYGPNDNFHPENSHVLPALMRRFHEAKVNGAKEVVVWGTGSPLREFLHVDDLADAVVFMMEKYSGVEHLNVGSGKEVTIKELAESMKEVVGFEGDLVWDSTKPDGTPRKLMDSSKLAALGWTPKVSLKDGLVDTYKWYLENVKQ*