Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1398530 | XP_003590996.1 | 97.458 | 118 | 2 | 1 | 1 | 118 | 95 | 211 | 3.25e-68 | 214 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1398530 | G7I6V2 | 97.458 | 118 | 2 | 1 | 1 | 118 | 95 | 211 | 1.55e-68 | 214 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0032180 | Msa1398530 | 0.825879 | 3.685208e-54 | -8.615850e-47 |
| Msa0827420 | Msa1398530 | 0.812049 | 5.156033e-51 | -8.615850e-47 |
| Msa0172590 | Msa1398530 | 0.817876 | 2.627146e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1398530 | MtrunA17_Chr1g0190481 | 97.458 | 118 | 2 | 1 | 1 | 118 | 95 | 211 | 2.99e-72 | 214 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 32 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGACACAACAACAATTAA+TGG | 0.291040 | tig0022254:-103946 | Msa1398530:CDS |
| ACCCTTTACCTAAAATCTTC+TGG | 0.314141 | tig0022254:-103648 | Msa1398530:CDS |
| GTTGGAGGCAATTCATCGTT+AGG | 0.453003 | tig0022254:-103801 | Msa1398530:CDS |
| GGCAATTCATCGTTAGGTAC+TGG | 0.456481 | tig0022254:-103795 | Msa1398530:CDS |
| GGTCGTAGCTCGGCTGGTGA+TGG | 0.457573 | tig0022254:-103756 | Msa1398530:CDS |
| TACCTAAAATCTTCTGGTGA+TGG | 0.461677 | tig0022254:-103642 | Msa1398530:CDS |
| ACCTAAAATCTTCTGGTGAT+GGG | 0.465114 | tig0022254:-103641 | Msa1398530:CDS |
| GCCGAGCTACGACCAAAATC+AGG | 0.475093 | tig0022254:+103765 | None:intergenic |
| CACTCGAATTCATACCGAGT+TGG | 0.508517 | tig0022254:+103865 | None:intergenic |
| CTTCACATGTTGCATAGTGA+AGG | 0.521552 | tig0022254:-103831 | Msa1398530:CDS |
| CATAGTGAAGGGGCTAATGT+TGG | 0.526222 | tig0022254:-103819 | Msa1398530:CDS |
| CAAGACATTGGAAGTGCTGA+TGG | 0.526599 | tig0022254:-103714 | Msa1398530:CDS |
| GGCCGCGGTGGAAGCTCTAG+TGG | 0.537316 | tig0022254:-103693 | Msa1398530:CDS |
| CAAGCCTTGCAAAGCCAACT+CGG | 0.540362 | tig0022254:-103879 | Msa1398530:CDS |
| CTTCACTATGCAACATGTGA+AGG | 0.550899 | tig0022254:+103832 | None:intergenic |
| AGTGAAGGGGCTAATGTTGG+AGG | 0.551470 | tig0022254:-103816 | Msa1398530:CDS |
| TTCACATGTTGCATAGTGAA+GGG | 0.554627 | tig0022254:-103830 | Msa1398530:CDS |
| CATACCGAGTTGGCTTTGCA+AGG | 0.565479 | tig0022254:+103875 | None:intergenic |
| GGTGATGGTTTGCACGGTAG+TGG | 0.566282 | tig0022254:-103741 | Msa1398530:CDS |
| GGTAGTGGCAAGCAAGACAT+TGG | 0.567432 | tig0022254:-103726 | Msa1398530:CDS |
| TCTAGTGGTCACTCTGGTGA+TGG | 0.573573 | tig0022254:-103678 | Msa1398530:CDS |
| ATTGGAAGTGCTGATGGCCG+CGG | 0.585066 | tig0022254:-103708 | Msa1398530:CDS |
| TCGGCTGGTGATGGTTTGCA+CGG | 0.610652 | tig0022254:-103747 | Msa1398530:CDS |
| AATTCATCGTTAGGTACTGG+AGG | 0.617510 | tig0022254:-103792 | Msa1398530:CDS |
| GGAAGCTCTAGTGGTCACTC+TGG | 0.631800 | tig0022254:-103684 | Msa1398530:CDS |
| ATGAATTCGAGTGGAAGTCA+AGG | 0.645385 | tig0022254:-103855 | Msa1398530:CDS |
| TCACATGTTGCATAGTGAAG+GGG | 0.652179 | tig0022254:-103829 | Msa1398530:CDS |
| AGTGGTCACTCTGGTGATGG+CGG | 0.666560 | tig0022254:-103675 | Msa1398530:CDS |
| CTGTTGAGCATACATAAGAG+AGG | 0.666776 | tig0022254:+103914 | None:intergenic |
| CAACTCGGTATGAATTCGAG+TGG | 0.681006 | tig0022254:-103864 | Msa1398530:CDS |
| GGAAGTGCTGATGGCCGCGG+TGG | 0.694316 | tig0022254:-103705 | Msa1398530:CDS |
| GACCACTAGAGCTTCCACCG+CGG | 0.784566 | tig0022254:+103691 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GATGACACAACAACAATTAA+TGG | - | tig0022254:103658-103677 | Msa1398530:CDS | 30.0% | |
| !! | ACCAGAAGATTTTAGGTAAA+GGG | + | tig0022254:103960-103979 | None:intergenic | 30.0% |
| TTCACATGTTGCATAGTGAA+GGG | - | tig0022254:103774-103793 | Msa1398530:CDS | 35.0% | |
| ACCCTTTACCTAAAATCTTC+TGG | - | tig0022254:103956-103975 | Msa1398530:CDS | 35.0% | |
| !! | CACCAGAAGATTTTAGGTAA+AGG | + | tig0022254:103961-103980 | None:intergenic | 35.0% |
| TACCTAAAATCTTCTGGTGA+TGG | - | tig0022254:103962-103981 | Msa1398530:CDS | 35.0% | |
| ACCTAAAATCTTCTGGTGAT+GGG | - | tig0022254:103963-103982 | Msa1398530:CDS | 35.0% | |
| CTGTTGAGCATACATAAGAG+AGG | + | tig0022254:103693-103712 | None:intergenic | 40.0% | |
| ATGAATTCGAGTGGAAGTCA+AGG | - | tig0022254:103749-103768 | Msa1398530:CDS | 40.0% | |
| CTTCACTATGCAACATGTGA+AGG | + | tig0022254:103775-103794 | None:intergenic | 40.0% | |
| CTTCACATGTTGCATAGTGA+AGG | - | tig0022254:103773-103792 | Msa1398530:CDS | 40.0% | |
| TCACATGTTGCATAGTGAAG+GGG | - | tig0022254:103775-103794 | Msa1398530:CDS | 40.0% | |
| !! | AATTCATCGTTAGGTACTGG+AGG | - | tig0022254:103812-103831 | Msa1398530:CDS | 40.0% |
| ! | ACTGGAGGTTTTCCTGATTT+TGG | - | tig0022254:103827-103846 | Msa1398530:CDS | 40.0% |
| ! | TCCCATCACCAGAAGATTTT+AGG | + | tig0022254:103967-103986 | None:intergenic | 40.0% |
| CACTCGAATTCATACCGAGT+TGG | + | tig0022254:103742-103761 | None:intergenic | 45.0% | |
| CAACTCGGTATGAATTCGAG+TGG | - | tig0022254:103740-103759 | Msa1398530:CDS | 45.0% | |
| CATAGTGAAGGGGCTAATGT+TGG | - | tig0022254:103785-103804 | Msa1398530:CDS | 45.0% | |
| GTTGGAGGCAATTCATCGTT+AGG | - | tig0022254:103803-103822 | Msa1398530:CDS | 45.0% | |
| GGCAATTCATCGTTAGGTAC+TGG | - | tig0022254:103809-103828 | Msa1398530:CDS | 45.0% | |
| !! | TCCTGATTTTGGTCGTAGCT+CGG | - | tig0022254:103838-103857 | Msa1398530:CDS | 45.0% |
| !! | CAAGACATTGGAAGTGCTGA+TGG | - | tig0022254:103890-103909 | Msa1398530:CDS | 45.0% |
| CAAGCCTTGCAAAGCCAACT+CGG | - | tig0022254:103725-103744 | Msa1398530:CDS | 50.0% | |
| !! | CATACCGAGTTGGCTTTGCA+AGG | + | tig0022254:103732-103751 | None:intergenic | 50.0% |
| AGTGAAGGGGCTAATGTTGG+AGG | - | tig0022254:103788-103807 | Msa1398530:CDS | 50.0% | |
| ! | GGTAGTGGCAAGCAAGACAT+TGG | - | tig0022254:103878-103897 | Msa1398530:CDS | 50.0% |
| TCTAGTGGTCACTCTGGTGA+TGG | - | tig0022254:103926-103945 | Msa1398530:CDS | 50.0% | |
| GCCGAGCTACGACCAAAATC+AGG | + | tig0022254:103842-103861 | None:intergenic | 55.0% | |
| !! | GATTTTGGTCGTAGCTCGGC+TGG | - | tig0022254:103842-103861 | Msa1398530:CDS | 55.0% |
| !! | TCGGCTGGTGATGGTTTGCA+CGG | - | tig0022254:103857-103876 | Msa1398530:CDS | 55.0% |
| ! | GGTGATGGTTTGCACGGTAG+TGG | - | tig0022254:103863-103882 | Msa1398530:CDS | 55.0% |
| !! | ATTGGAAGTGCTGATGGCCG+CGG | - | tig0022254:103896-103915 | Msa1398530:CDS | 55.0% |
| ! | GGAAGCTCTAGTGGTCACTC+TGG | - | tig0022254:103920-103939 | Msa1398530:CDS | 55.0% |
| !! | AGTGGTCACTCTGGTGATGG+CGG | - | tig0022254:103929-103948 | Msa1398530:CDS | 55.0% |
| GACCACTAGAGCTTCCACCG+CGG | + | tig0022254:103916-103935 | None:intergenic | 60.0% | |
| GGTCGTAGCTCGGCTGGTGA+TGG | - | tig0022254:103848-103867 | Msa1398530:CDS | 65.0% | |
| !! | GGAAGTGCTGATGGCCGCGG+TGG | - | tig0022254:103899-103918 | Msa1398530:CDS | 70.0% |
| ! | GGCCGCGGTGGAAGCTCTAG+TGG | - | tig0022254:103911-103930 | Msa1398530:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0022254 | gene | 103635 | 103991 | 103635 | ID=Msa1398530;Name=Msa1398530 |
| tig0022254 | mRNA | 103635 | 103991 | 103635 | ID=Msa1398530-mRNA-1;Parent=Msa1398530;Name=Msa1398530-mRNA-1;_AED=0.03;_eAED=0.03;_QI=0|-1|0|1|-1|1|1|0|118 |
| tig0022254 | exon | 103635 | 103991 | 103635 | ID=Msa1398530-mRNA-1:exon:16670;Parent=Msa1398530-mRNA-1 |
| tig0022254 | CDS | 103635 | 103991 | 103635 | ID=Msa1398530-mRNA-1:cds;Parent=Msa1398530-mRNA-1 |
| Gene Sequence |
| Protein sequence |
