AlfalfaGEDB | Msa0827420

M. sativa cultivar zhongmu-4 / Msa0827420

KEGG: -
GO: GO:0003002 GO:0003674 GO:0003712 GO:0003713 GO:0007275 GO:0007389 GO:0008150 GO:0008283 GO:0009889 GO:0009891 GO:0009893 GO:0009955 GO:0010556 GO:0010557 GO:0010604 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0045935 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0099402 GO:0140110 GO:1902680 GO:1903506 GO:1903508 GO:2001141
PFAM: PF05030.15

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0827420	XP_003590996.1	97.653	213	3	2	1	213	1	211	6.11e-142	405

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0827420	sp\|Q8L8A5\|GIF1_ARATH	58.798	233	53	8	1	213	1	210	1.55e-54	176

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0827420	G7I6V2	97.653	213	3	2	1	213	1	211	2.92e-142	405

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0015570	Msa0827420	0.807189	5.707063e-50	-8.615850e-47
Msa0030070	Msa0827420	0.805461	1.320249e-49	-8.615850e-47
Msa0032180	Msa0827420	0.865245	6.789678e-65	-8.615850e-47
Msa0044520	Msa0827420	0.800171	1.633520e-48	-8.615850e-47
Msa0050530	Msa0827420	0.828235	1.006561e-54	-8.615850e-47
Msa0079410	Msa0827420	0.886147	4.402481e-72	-8.615850e-47
Msa0091350	Msa0827420	0.803791	2.945286e-49	-8.615850e-47
Msa0100600	Msa0827420	0.843552	1.306099e-58	-8.615850e-47
Msa0100890	Msa0827420	0.820479	6.711876e-53	-8.615850e-47
Msa0109730	Msa0827420	0.802928	4.444515e-49	-8.615850e-47
Msa0605520	Msa0827420	0.820681	6.030840e-53	-8.615850e-47
Msa0615630	Msa0827420	0.815474	9.073784e-52	-8.615850e-47
Msa0620500	Msa0827420	0.820327	7.271505e-53	-8.615850e-47
Msa0625340	Msa0827420	0.823195	1.578132e-53	-8.615850e-47
Msa0640800	Msa0827420	0.827843	1.250563e-54	-8.615850e-47
Msa0646260	Msa0827420	0.808627	2.822263e-50	-8.615850e-47
Msa0647440	Msa0827420	0.819087	1.396024e-52	-8.615850e-47
Msa0648760	Msa0827420	0.813438	2.559763e-51	-8.615850e-47
Msa0654740	Msa0827420	0.823060	1.696819e-53	-8.615850e-47
Msa0657210	Msa0827420	0.810460	1.140216e-50	-8.615850e-47
Msa0660260	Msa0827420	0.820312	7.331596e-53	-8.615850e-47
Msa0663290	Msa0827420	0.807128	5.878961e-50	-8.615850e-47
Msa0664070	Msa0827420	0.804464	2.133805e-49	-8.615850e-47
Msa0685610	Msa0827420	0.809224	2.102731e-50	-8.615850e-47
Msa0687090	Msa0827420	0.807482	4.946029e-50	-8.615850e-47
Msa0688380	Msa0827420	0.812013	5.248718e-51	-8.615850e-47
Msa0688620	Msa0827420	0.840746	7.220497e-58	-8.615850e-47
Msa0690320	Msa0827420	0.804109	2.529549e-49	-8.615850e-47
Msa0690570	Msa0827420	0.809055	2.285990e-50	-8.615850e-47
Msa0691270	Msa0827420	0.800298	1.538964e-48	-8.615850e-47
Msa0701250	Msa0827420	0.815822	7.592919e-52	-8.615850e-47
Msa0707260	Msa0827420	0.846482	2.112361e-59	-8.615850e-47
Msa0717900	Msa0827420	0.809416	1.912601e-50	-8.615850e-47
Msa0720280	Msa0827420	0.851065	1.131436e-60	-8.615850e-47
Msa0737130	Msa0827420	0.813765	2.168732e-51	-8.615850e-47
Msa0749190	Msa0827420	0.840498	8.387432e-58	-8.615850e-47
Msa0750520	Msa0827420	0.838748	2.391000e-57	-8.615850e-47
Msa0751720	Msa0827420	0.870009	2.016038e-66	-8.615850e-47
Msa0752230	Msa0827420	0.810064	1.388055e-50	-8.615850e-47
Msa0774060	Msa0827420	0.825188	5.370532e-54	-8.615850e-47
Msa0778600	Msa0827420	0.811255	7.669787e-51	-8.615850e-47
Msa0780820	Msa0827420	0.835272	1.848056e-56	-8.615850e-47
Msa0798080	Msa0827420	0.812370	4.387039e-51	-8.615850e-47
Msa0803140	Msa0827420	0.847143	1.392998e-59	-8.615850e-47
Msa0816080	Msa0827420	0.811386	7.183848e-51	-8.615850e-47
Msa0816230	Msa0827420	0.838720	2.432061e-57	-8.615850e-47
Msa0822510	Msa0827420	0.810014	1.422872e-50	-8.615850e-47
Msa0827420	Msa0831820	0.823419	1.399468e-53	-8.615850e-47
Msa0827420	Msa0837680	0.813351	2.675175e-51	-8.615850e-47
Msa0827420	Msa0841500	0.827822	1.264852e-54	-8.615850e-47
Msa0827420	Msa0861440	0.817518	3.163787e-52	-8.615850e-47
Msa0827420	Msa0866530	0.803887	2.812803e-49	-8.615850e-47
Msa0827420	Msa0876630	0.816626	5.018311e-52	-8.615850e-47
Msa0827420	Msa0900090	0.817870	2.635174e-52	-8.615850e-47
Msa0827420	Msa0901150	0.845088	5.049833e-59	-8.615850e-47
Msa0827420	Msa0909760	0.823796	1.142010e-53	-8.615850e-47
Msa0827420	Msa0926080	0.840754	7.186087e-58	-8.615850e-47
Msa0827420	Msa0926310	0.804987	1.659102e-49	-8.615850e-47
Msa0827420	Msa0929900	0.812714	3.689906e-51	-8.615850e-47
Msa0827420	Msa0930040	0.800489	1.406897e-48	-8.615850e-47
Msa0827420	Msa0965470	0.804335	2.270071e-49	-8.615850e-47
Msa0827420	Msa0966710	0.848649	5.357174e-60	-8.615850e-47
Msa0827420	Msa0970040	0.815701	8.079381e-52	-8.615850e-47
Msa0827420	Msa0978980	0.801706	7.932495e-49	-8.615850e-47
Msa0827420	Msa1000970	0.848009	8.051034e-60	-8.615850e-47
Msa0827420	Msa1008670	0.817263	3.610304e-52	-8.615850e-47
Msa0827420	Msa1008850	0.822899	1.850458e-53	-8.615850e-47
Msa0827420	Msa1027160	0.813585	2.375856e-51	-8.615850e-47
Msa0827420	Msa1071410	0.839602	1.436071e-57	-8.615850e-47
Msa0827420	Msa1076020	0.805987	1.023616e-49	-8.615850e-47
Msa0827420	Msa1078880	0.803140	4.017686e-49	-8.615850e-47
Msa0827420	Msa1085680	0.816448	5.500614e-52	-8.615850e-47
Msa0827420	Msa1106220	0.814163	1.771737e-51	-8.615850e-47
Msa0827420	Msa1127280	0.804702	1.902768e-49	-8.615850e-47
Msa0827420	Msa1144990	0.834167	3.505798e-56	-8.615850e-47
Msa0827420	Msa1154630	0.836800	7.566677e-57	-8.615850e-47
Msa0827420	Msa1162880	0.800446	1.435727e-48	-8.615850e-47
Msa0827420	Msa1163180	0.812704	3.708103e-51	-8.615850e-47
Msa0827420	Msa1165480	0.807809	4.215355e-50	-8.615850e-47
Msa0827420	Msa1169190	0.810161	1.322991e-50	-8.615850e-47
Msa0827420	Msa1206040	0.810291	1.239880e-50	-8.615850e-47
Msa0827420	Msa1212700	0.817312	3.521038e-52	-8.615850e-47
Msa0827420	Msa1212920	0.833146	6.307295e-56	-8.615850e-47
Msa0827420	Msa1215980	0.822262	2.602696e-53	-8.615850e-47
Msa0827420	Msa1233780	0.816161	6.378722e-52	-8.615850e-47
Msa0827420	Msa1254420	0.802955	4.389018e-49	-8.615850e-47
Msa0827420	Msa1267930	0.815616	8.438254e-52	-8.615850e-47
Msa0827420	Msa1276090	0.804820	1.797945e-49	-8.615850e-47
Msa0827420	Msa1277110	0.825453	4.648677e-54	-8.615850e-47
Msa0827420	Msa1294790	0.813905	2.019957e-51	-8.615850e-47
Msa0827420	Msa1295670	0.800509	1.393775e-48	-8.615850e-47
Msa0827420	Msa1297070	0.812127	4.956803e-51	-8.615850e-47
Msa0827420	Msa1299980	0.800055	1.724877e-48	-8.615850e-47
Msa0827420	Msa1308850	0.811204	7.868988e-51	-8.615850e-47
Msa0827420	Msa1308860	0.814358	1.604978e-51	-8.615850e-47
Msa0827420	Msa1315500	0.813204	2.881776e-51	-8.615850e-47
Msa0827420	Msa1328870	0.810586	1.070852e-50	-8.615850e-47
Msa0827420	Msa1331650	0.817744	2.813549e-52	-8.615850e-47
Msa0827420	Msa1333500	0.817710	2.862794e-52	-8.615850e-47
Msa0827420	Msa1339230	0.823525	1.321780e-53	-8.615850e-47
Msa0827420	Msa1342810	0.821896	3.164385e-53	-8.615850e-47
Msa0827420	Msa1356450	0.801159	1.027133e-48	-8.615850e-47
Msa0827420	Msa1361930	0.803102	4.092538e-49	-8.615850e-47
Msa0827420	Msa1368170	0.821628	3.648892e-53	-8.615850e-47
Msa0827420	Msa1372030	0.824305	8.674572e-54	-8.615850e-47
Msa0827420	Msa1381800	0.839611	1.429044e-57	-8.615850e-47
Msa0827420	Msa1385570	0.807495	4.915500e-50	-8.615850e-47
Msa0827420	Msa1387500	0.805164	1.523591e-49	-8.615850e-47
Msa0827420	Msa1389820	0.834097	3.649422e-56	-8.615850e-47
Msa0827420	Msa1393560	0.837118	6.276370e-57	-8.615850e-47
Msa0827420	Msa1398530	0.812049	5.156033e-51	-8.615850e-47
Msa0827420	Msa1408160	0.802143	6.452248e-49	-8.615850e-47
Msa0827420	Msa1420830	0.815205	1.041333e-51	-8.615850e-47
Msa0827420	Msa1426320	0.826572	2.520951e-54	-8.615850e-47
Msa0827420	Msa1448960	0.807205	5.664450e-50	-8.615850e-47
Msa0125590	Msa0827420	0.819183	1.327513e-52	-8.615850e-47
Msa0127340	Msa0827420	0.801276	9.720971e-49	-8.615850e-47
Msa0156050	Msa0827420	0.804262	2.350218e-49	-8.615850e-47
Msa0156090	Msa0827420	0.809520	1.816571e-50	-8.615850e-47
Msa0168380	Msa0827420	0.810304	1.232025e-50	-8.615850e-47
Msa0170070	Msa0827420	0.821518	3.868852e-53	-8.615850e-47
Msa0172590	Msa0827420	0.981713	2.906452e-153	-8.615850e-47
Msa0195440	Msa0827420	0.822311	2.535035e-53	-8.615850e-47
Msa0204970	Msa0827420	0.822247	2.624031e-53	-8.615850e-47
Msa0218020	Msa0827420	0.822118	2.809947e-53	-8.615850e-47
Msa0221180	Msa0827420	0.814422	1.553126e-51	-8.615850e-47
Msa0225800	Msa0827420	0.814960	1.180817e-51	-8.615850e-47
Msa0237240	Msa0827420	0.810689	1.017577e-50	-8.615850e-47
Msa0364680	Msa0827420	0.851481	8.629198e-61	-8.615850e-47
Msa0373170	Msa0827420	0.847770	9.373125e-60	-8.615850e-47
Msa0382230	Msa0827420	0.800563	1.359245e-48	-8.615850e-47
Msa0388250	Msa0827420	0.848245	6.931474e-60	-8.615850e-47
Msa0404770	Msa0827420	0.826522	2.590645e-54	-8.615850e-47
Msa0415820	Msa0827420	0.811776	5.909660e-51	-8.615850e-47
Msa0416210	Msa0827420	0.824677	7.089396e-54	-8.615850e-47
Msa0421750	Msa0827420	0.834064	3.720611e-56	-8.615850e-47
Msa0430080	Msa0827420	0.804721	1.885403e-49	-8.615850e-47
Msa0430170	Msa0827420	0.801329	9.481040e-49	-8.615850e-47
Msa0433630	Msa0827420	0.830935	2.218248e-55	-8.615850e-47
Msa0466520	Msa0827420	0.805880	1.078010e-49	-8.615850e-47
Msa0467690	Msa0827420	0.850851	1.299334e-60	-8.615850e-47
Msa0477480	Msa0827420	0.802469	5.527519e-49	-8.615850e-47
Msa0249870	Msa0827420	0.814515	1.481019e-51	-8.615850e-47
Msa0260630	Msa0827420	0.805814	1.113193e-49	-8.615850e-47
Msa0262750	Msa0827420	0.816458	5.472573e-52	-8.615850e-47
Msa0272540	Msa0827420	0.802830	4.655903e-49	-8.615850e-47
Msa0281540	Msa0827420	0.835326	1.791005e-56	-8.615850e-47
Msa0288400	Msa0827420	0.804554	2.043419e-49	-8.615850e-47
Msa0300470	Msa0827420	0.807319	5.356177e-50	-8.615850e-47
Msa0312740	Msa0827420	0.824014	1.015165e-53	-8.615850e-47
Msa0320580	Msa0827420	0.828432	9.021439e-55	-8.615850e-47
Msa0337970	Msa0827420	0.806377	8.474930e-50	-8.615850e-47
Msa0342220	Msa0827420	0.818821	1.604938e-52	-8.615850e-47
Msa0344110	Msa0827420	0.834789	2.446603e-56	-8.615850e-47
Msa0506880	Msa0827420	0.822801	1.949907e-53	-8.615850e-47
Msa0507810	Msa0827420	0.812848	3.447787e-51	-8.615850e-47
Msa0507820	Msa0827420	0.814035	1.891345e-51	-8.615850e-47
Msa0507920	Msa0827420	0.811373	7.231956e-51	-8.615850e-47
Msa0514020	Msa0827420	0.841137	5.701908e-58	-8.615850e-47
Msa0521510	Msa0827420	0.837208	5.952700e-57	-8.615850e-47
Msa0521530	Msa0827420	0.816873	4.417595e-52	-8.615850e-47
Msa0527850	Msa0827420	0.833915	4.054237e-56	-8.615850e-47
Msa0537990	Msa0827420	0.823317	1.478483e-53	-8.615850e-47
Msa0541740	Msa0827420	0.818382	2.017917e-52	-8.615850e-47
Msa0541880	Msa0827420	0.822749	2.005755e-53	-8.615850e-47
Msa0544410	Msa0827420	0.826812	2.209218e-54	-8.615850e-47
Msa0554300	Msa0827420	0.812132	4.945573e-51	-8.615850e-47
Msa0561560	Msa0827420	0.823632	1.247816e-53	-8.615850e-47
Msa0564980	Msa0827420	0.809852	1.541394e-50	-8.615850e-47
Msa0571350	Msa0827420	0.833498	5.150979e-56	-8.615850e-47
Msa0572830	Msa0827420	0.852546	4.300465e-61	-8.615850e-47
Msa0574660	Msa0827420	0.835824	1.339859e-56	-8.615850e-47
Msa0575710	Msa0827420	0.830165	3.424918e-55	-8.615850e-47
Msa0576330	Msa0827420	0.821127	4.762751e-53	-8.615850e-47
Msa0580940	Msa0827420	0.804412	2.187011e-49	-8.615850e-47
Msa0580990	Msa0827420	0.804339	2.265075e-49	-8.615850e-47
Msa0582460	Msa0827420	0.825811	3.824280e-54	-8.615850e-47
Msa0586200	Msa0827420	0.815831	7.555395e-52	-8.615850e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0827420	MtrunA17_Chr1g0190481	97.653	213	3	2	1	213	1	211	5.62e-146	405
Msa0827420	MtrunA17_Chr7g0274341	64.378	233	57	10	1	213	1	227	1.66e-62	194
Msa0827420	MtrunA17_Chr8g0346111	59.140	93	33	4	7	97	6	95	8.05e-26	99.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa0827420	AT5G28640.1	58.798	233	53	8	1	213	1	210	1.57e-55	176
Msa0827420	AT4G00850.1	38.428	229	95	10	7	203	6	220	9.05e-27	102
Msa0827420	AT4G00850.2	51.327	113	42	4	7	107	6	117	1.06e-24	97.1
Msa0827420	AT1G01160.1	61.644	73	26	2	2	73	3	74	3.14e-21	87.4
Msa0827420	AT1G01160.2	42.453	106	27	2	2	73	3	108	4.21e-16	74.7
Msa0827420	AT5G28640.2	47.059	170	48	7	64	213	1	148	3.42e-11	59.7
Msa0827420	AT5G28640.3	47.059	170	48	7	64	213	1	148	3.42e-11	59.7

Find 77 sgRNAs with CRISPR-Local

Find 303 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATGCTCTTGTGTAAAGTTT+TGG	0.241797	5_3:-68583498	Msa0827420:three_prime_UTR
CCTAATCCTCCTAACTAAAA+AGG	0.244213	5_3:+68583667	None:intergenic
GATGACACAACAACAATTAA+TGG	0.291040	5_3:-68584035	Msa0827420:CDS
CGGTGAGCTTGCCAGAGTTC+TGG	0.291829	5_3:+68585067	None:intergenic
GTTTCTTTCTGCTCTTGTTC+AGG	0.296487	5_3:-68585444	Msa0827420:five_prime_UTR
ACCCTTTACCTAAAATCTTC+TGG	0.314141	5_3:-68583737	Msa0827420:CDS
CAGGGTCACACACCTTTGTT+TGG	0.314789	5_3:-68585418	Msa0827420:five_prime_UTR
TCTGCTCTTGTTCAGGTCAC+AGG	0.322676	5_3:-68585437	Msa0827420:five_prime_UTR
GCCAGGCATAGTAGGTGGTT+GGG	0.340247	5_3:+68584600	None:intergenic
GATTTCTCTGCAGCCTTGAT+TGG	0.360123	5_3:+68584656	None:intergenic
TCATGGGCTGCATCTGCATC+AGG	0.386454	5_3:+68585345	None:intergenic
GGCCAGGCATAGTAGGTGGT+TGG	0.392149	5_3:+68584599	None:intergenic
TGATCAGTAGTGACGTTGTT+AGG	0.399937	5_3:+68585308	None:intergenic
TGCTGCATCATTCCACTTGA+AGG	0.403024	5_3:+68584094	None:intergenic
AATGAGGTTGCTCAATTTCA+AGG	0.409443	5_3:-68583469	Msa0827420:three_prime_UTR
TGGTAAAACTAATTAGTTCC+TGG	0.422692	5_3:-68583690	Msa0827420:three_prime_UTR
TTCAACAATCTTGAGAATCA+AGG	0.434695	5_3:+68585092	None:intergenic
CACTACTGATCATATTCAAC+AGG	0.437472	5_3:-68585297	Msa0827420:intron
TGTACCTGGCCAGGCATAGT+AGG	0.442060	5_3:+68584592	None:intergenic
CTGCTCTTGTTCAGGTCACA+GGG	0.442934	5_3:-68585436	Msa0827420:five_prime_UTR
GGGAATTAGTTAAGCAAGAA+TGG	0.444582	5_3:-68583710	Msa0827420:exon
CCAGGCATAGTAGGTGGTTG+GGG	0.455596	5_3:+68584601	None:intergenic
GGCAATTCATCGTTAGGTAC+TGG	0.456481	5_3:-68583884	Msa0827420:CDS
GGTCGTAGCTCGGCTGGTGA+TGG	0.457573	5_3:-68583845	Msa0827420:CDS
TACCTAAAATCTTCTGGTGA+TGG	0.461677	5_3:-68583731	Msa0827420:CDS
ACCTAAAATCTTCTGGTGAT+GGG	0.465114	5_3:-68583730	Msa0827420:CDS
GCCGAGCTACGACCAAAATC+AGG	0.475093	5_3:+68583854	None:intergenic
GTTGGAGGCAATTCATCGTT+AGG	0.483523	5_3:-68583890	Msa0827420:CDS
ATAATTTCAGGAACCAATCA+AGG	0.484218	5_3:-68584669	Msa0827420:intron
TTTCTTTGAGGCGGCTAAAG+AGG	0.487663	5_3:+68585569	None:intergenic
CCCCAACCACCTACTATGCC+TGG	0.488367	5_3:-68584601	Msa0827420:CDS
AGATAGGGGTGTACCTGGCC+AGG	0.491173	5_3:+68584583	None:intergenic
GGATAGTAAGCTGCCATCAT+GGG	0.492977	5_3:+68585329	None:intergenic
TTCAAGTGGAATGATGCAGC+AGG	0.504147	5_3:-68584092	Msa0827420:CDS
CACTCGAATTCATACCGAGT+TGG	0.508517	5_3:+68583954	None:intergenic
AGGATAGTAAGCTGCCATCA+TGG	0.510940	5_3:+68585328	None:intergenic
CTTCACATGTTGCATAGTGA+AGG	0.521552	5_3:-68583920	Msa0827420:CDS
CAAGACATTGGAAGTGCTGA+TGG	0.526599	5_3:-68583803	Msa0827420:CDS
AGTGAAGGGGCTAACGTTGG+AGG	0.535342	5_3:-68583905	Msa0827420:CDS
GGCCGCGGTGGAAGCTCTAG+TGG	0.537316	5_3:-68583782	Msa0827420:CDS
AACAGAGAAACAGAAGAGTC+TGG	0.538077	5_3:+68585474	None:intergenic
CAAGCCTTGCAAAGCCAACT+CGG	0.540362	5_3:-68583968	Msa0827420:CDS
GAGGCAATGGTAGTATTATG+AGG	0.542912	5_3:+68585588	None:intergenic
AGTTACTAGTACACAAACAT+AGG	0.546361	5_3:+68583534	None:intergenic
CTTCACTATGCAACATGTGA+AGG	0.550899	5_3:+68583921	None:intergenic
CTGTTCTTACTCAGCACACT+CGG	0.552381	5_3:+68585047	None:intergenic
TTCACATGTTGCATAGTGAA+GGG	0.554627	5_3:-68583919	Msa0827420:CDS
ATTGTTGAAAGCCAGAACTC+TGG	0.560643	5_3:-68585078	Msa0827420:CDS
ACCACCTACTATGCCTGGCC+AGG	0.560989	5_3:-68584596	Msa0827420:intron
CTACACAACACTTAGAAGAG+TGG	0.563660	5_3:+68585544	None:intergenic
CATACCGAGTTGGCTTTGCA+AGG	0.565479	5_3:+68583964	None:intergenic
GGTGATGGTTTGCACGGTAG+TGG	0.566282	5_3:-68583830	Msa0827420:CDS
GGTAGTGGCAAGCAAGACAT+TGG	0.567432	5_3:-68583815	Msa0827420:CDS
TCTAGTGGTCACTCTGGTGA+TGG	0.573573	5_3:-68583767	Msa0827420:CDS
ATTGGAAGTGCTGATGGCCG+CGG	0.585066	5_3:-68583797	Msa0827420:CDS
GAGAATCAGCTATTGCAGCT+AGG	0.595794	5_3:+68584626	None:intergenic
ACATTGCAGTACCCTTCAAG+TGG	0.608273	5_3:-68584106	Msa0827420:intron
TCGGCTGGTGATGGTTTGCA+CGG	0.610652	5_3:-68583836	Msa0827420:CDS
GCTGCATCATTCCACTTGAA+GGG	0.611938	5_3:+68584095	None:intergenic
CATAGTGAAGGGGCTAACGT+TGG	0.615754	5_3:-68583908	Msa0827420:CDS
AATTCATCGTTAGGTACTGG+AGG	0.617510	5_3:-68583881	Msa0827420:CDS
TGAGGCGGCTAAAGAGGCAA+TGG	0.620919	5_3:+68585575	None:intergenic
GGAAGCTCTAGTGGTCACTC+TGG	0.631800	5_3:-68583773	Msa0827420:CDS
ACCTGGCCAGGCATAGTAGG+TGG	0.636818	5_3:+68584595	None:intergenic
CTAAGTTCAAATCCAAACAA+AGG	0.637687	5_3:+68585406	None:intergenic
ATGAATTCGAGTGGAAGTCA+AGG	0.645385	5_3:-68583944	Msa0827420:CDS
GATGCAGATGCAGCCCATGA+TGG	0.647712	5_3:-68585342	Msa0827420:CDS
GCAGGGAGGACACTACATGC+AGG	0.651199	5_3:-68584074	Msa0827420:CDS
TCACATGTTGCATAGTGAAG+GGG	0.652179	5_3:-68583918	Msa0827420:CDS
ACATTAAGTTAAGTAGCTGA+AGG	0.653572	5_3:-68583638	Msa0827420:three_prime_UTR
AGTGGTCACTCTGGTGATGG+CGG	0.666560	5_3:-68583764	Msa0827420:CDS
CTGTTGAGCATACATAAGAG+AGG	0.666776	5_3:+68584003	None:intergenic
TCAAGTGGAATGATGCAGCA+GGG	0.669197	5_3:-68584091	Msa0827420:CDS
CAACTCGGTATGAATTCGAG+TGG	0.681006	5_3:-68583953	Msa0827420:CDS
GGAAGTGCTGATGGCCGCGG+TGG	0.694316	5_3:-68583794	Msa0827420:CDS
AGTGGAATGATGCAGCAGGG+AGG	0.729356	5_3:-68584088	Msa0827420:CDS
GACCACTAGAGCTTCCACCG+CGG	0.784566	5_3:+68583780	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GAAAATAAATAGTTAAAAAA+AGG	+	chr5_3:68583887-68583906	None:intergenic	10.0%
!!!	TTGTTTTATGTTTAATTAAA+AGG	-	chr5_3:68585462-68585481	Msa0827420:five_prime_UTR	10.0%
!!	GAAAATAAATAGTTAAAAAA+AGG	+	chr5_3:68583887-68583906	None:intergenic	10.0%
!!!	TTGTTTTATGTTTAATTAAA+AGG	-	chr5_3:68585462-68585481	Msa0827420:five_prime_UTR	10.0%
!!!	TTTATTTTATTAAGGTTTGT+GGG	+	chr5_3:68584126-68584145	None:intergenic	15.0%
!!	ATATATACCTAAACAATTAT+TGG	-	chr5_3:68584343-68584362	Msa0827420:intron	15.0%
!!	AAATAATAGTAAAATGTATC+AGG	-	chr5_3:68584784-68584803	Msa0827420:intron	15.0%
!!!	TTTATTTTATTAAGGTTTGT+GGG	+	chr5_3:68584126-68584145	None:intergenic	15.0%
!!	ATATATACCTAAACAATTAT+TGG	-	chr5_3:68584343-68584362	Msa0827420:intron	15.0%
!!	AAATAATAGTAAAATGTATC+AGG	-	chr5_3:68584784-68584803	Msa0827420:intron	15.0%
!!!	TTATTTTATTAAGGTTTGTG+GGG	+	chr5_3:68584125-68584144	None:intergenic	20.0%
!!!	CTTTATTTTATTAAGGTTTG+TGG	+	chr5_3:68584127-68584146	None:intergenic	20.0%
!!!	TTTACTAGTATTAGCTTTTT+TGG	-	chr5_3:68584262-68584281	Msa0827420:intron	20.0%
!!	GTGAAAAATGAAAATTAGAA+AGG	+	chr5_3:68584619-68584638	None:intergenic	20.0%
!!	AAACATATTCATATGTACAA+GGG	+	chr5_3:68584712-68584731	None:intergenic	20.0%
!!	TAAACATATTCATATGTACA+AGG	+	chr5_3:68584713-68584732	None:intergenic	20.0%
!!	GTATTAATTAAGTGTACATT+TGG	-	chr5_3:68584759-68584778	Msa0827420:intron	20.0%
!!!	TTATTTTATTAAGGTTTGTG+GGG	+	chr5_3:68584125-68584144	None:intergenic	20.0%
!!!	CTTTATTTTATTAAGGTTTG+TGG	+	chr5_3:68584127-68584146	None:intergenic	20.0%
!!!	TTTACTAGTATTAGCTTTTT+TGG	-	chr5_3:68584262-68584281	Msa0827420:intron	20.0%
!!	GTGAAAAATGAAAATTAGAA+AGG	+	chr5_3:68584619-68584638	None:intergenic	20.0%
!!	AAACATATTCATATGTACAA+GGG	+	chr5_3:68584712-68584731	None:intergenic	20.0%
!!	TAAACATATTCATATGTACA+AGG	+	chr5_3:68584713-68584732	None:intergenic	20.0%
!!	GTATTAATTAAGTGTACATT+TGG	-	chr5_3:68584759-68584778	Msa0827420:intron	20.0%
!	TATAGCACCAATAATTGTTT+AGG	+	chr5_3:68584353-68584372	None:intergenic	25.0%
!!!	ATTATTGGTGCTATAATTTC+AGG	-	chr5_3:68584358-68584377	Msa0827420:intron	25.0%
!!	AACATTCTGAAAATAGATAG+GGG	+	chr5_3:68584473-68584492	None:intergenic	25.0%
!!	CAACATTCTGAAAATAGATA+GGG	+	chr5_3:68584474-68584493	None:intergenic	25.0%
!!	ACAACATTCTGAAAATAGAT+AGG	+	chr5_3:68584475-68584494	None:intergenic	25.0%
!	GAAAATTAGAAAGGAAACTA+GGG	+	chr5_3:68584610-68584629	None:intergenic	25.0%
!	TGAAAATTAGAAAGGAAACT+AGG	+	chr5_3:68584611-68584630	None:intergenic	25.0%
!	ATTAATACTACTAAGCATCA+TGG	+	chr5_3:68584747-68584766	None:intergenic	25.0%
!!	AACACAAATTTCTTCTAAAG+AGG	-	chr5_3:68584834-68584853	Msa0827420:intron	25.0%
!!	ACACAAATTTCTTCTAAAGA+GGG	-	chr5_3:68584835-68584854	Msa0827420:intron	25.0%
!	TATAGCACCAATAATTGTTT+AGG	+	chr5_3:68584353-68584372	None:intergenic	25.0%
!!!	ATTATTGGTGCTATAATTTC+AGG	-	chr5_3:68584358-68584377	Msa0827420:intron	25.0%
!!	AACATTCTGAAAATAGATAG+GGG	+	chr5_3:68584473-68584492	None:intergenic	25.0%
!!	CAACATTCTGAAAATAGATA+GGG	+	chr5_3:68584474-68584493	None:intergenic	25.0%
!!	ACAACATTCTGAAAATAGAT+AGG	+	chr5_3:68584475-68584494	None:intergenic	25.0%
!	GAAAATTAGAAAGGAAACTA+GGG	+	chr5_3:68584610-68584629	None:intergenic	25.0%
!	TGAAAATTAGAAAGGAAACT+AGG	+	chr5_3:68584611-68584630	None:intergenic	25.0%
!	ATTAATACTACTAAGCATCA+TGG	+	chr5_3:68584747-68584766	None:intergenic	25.0%
!!	AACACAAATTTCTTCTAAAG+AGG	-	chr5_3:68584834-68584853	Msa0827420:intron	25.0%
!!	ACACAAATTTCTTCTAAAGA+GGG	-	chr5_3:68584835-68584854	Msa0827420:intron	25.0%
	CTAAGTTCAAATCCAAACAA+AGG	+	chr5_3:68583636-68583655	None:intergenic	30.0%
	CCAATGGAATTGAAACAAAA+AGG	+	chr5_3:68583790-68583809	None:intergenic	30.0%
!!	CCTTTTTGTTTCAATTCCAT+TGG	-	chr5_3:68583787-68583806	Msa0827420:CDS	30.0%
	TTCAACAATCTTGAGAATCA+AGG	+	chr5_3:68583950-68583969	None:intergenic	30.0%
	AAGCCTGCAAAATATAGAAA+GGG	+	chr5_3:68584074-68584093	None:intergenic	30.0%
	AAAGCCTGCAAAATATAGAA+AGG	+	chr5_3:68584075-68584094	None:intergenic	30.0%
!!!	GTCATCATGTTTTGAAACTT+TGG	+	chr5_3:68584182-68584201	None:intergenic	30.0%
!	CTAAAATGTTGCAACCTTTT+TGG	-	chr5_3:68584208-68584227	Msa0827420:intron	30.0%
!!!	ACTAGTATTAGCTTTTTTGG+TGG	-	chr5_3:68584265-68584284	Msa0827420:intron	30.0%
!!!	CTAGTATTAGCTTTTTTGGT+GGG	-	chr5_3:68584266-68584285	Msa0827420:intron	30.0%
!!!	TAGTATTAGCTTTTTTGGTG+GGG	-	chr5_3:68584267-68584286	Msa0827420:intron	30.0%
	AGATCAAAATTCTGCTTACT+TGG	-	chr5_3:68584306-68584325	Msa0827420:intron	30.0%
	ATAATTTCAGGAACCAATCA+AGG	-	chr5_3:68584370-68584389	Msa0827420:intron	30.0%
	ACAAATCAATCATTGTTGTG+AGG	+	chr5_3:68584529-68584548	None:intergenic	30.0%
	ACATAATAAGAGCTAGAGTT+TGG	+	chr5_3:68584587-68584606	None:intergenic	30.0%
	AAAACATCTAGACACACAAT+AGG	+	chr5_3:68584682-68584701	None:intergenic	30.0%
	GGGTTTATATTTAGACACAT+AGG	-	chr5_3:68584855-68584874	Msa0827420:intron	30.0%
!	ATTACTGCTATTTGACCATT+TGG	-	chr5_3:68584891-68584910	Msa0827420:intron	30.0%
	GATGACACAACAACAATTAA+TGG	-	chr5_3:68585004-68585023	Msa0827420:intron	30.0%
!!	ACCAGAAGATTTTAGGTAAA+GGG	+	chr5_3:68585306-68585325	None:intergenic	30.0%
	TGGTAAAACTAATTAGTTCC+TGG	-	chr5_3:68585349-68585368	Msa0827420:CDS	30.0%
	ACATTAAGTTAAGTAGCTGA+AGG	-	chr5_3:68585401-68585420	Msa0827420:five_prime_UTR	30.0%
	AGTTACTAGTACACAAACAT+AGG	+	chr5_3:68585508-68585527	None:intergenic	30.0%
!	TATGCTCTTGTGTAAAGTTT+TGG	-	chr5_3:68585541-68585560	Msa0827420:five_prime_UTR	30.0%
	CTAAGTTCAAATCCAAACAA+AGG	+	chr5_3:68583636-68583655	None:intergenic	30.0%
	CCAATGGAATTGAAACAAAA+AGG	+	chr5_3:68583790-68583809	None:intergenic	30.0%
!!	CCTTTTTGTTTCAATTCCAT+TGG	-	chr5_3:68583787-68583806	Msa0827420:CDS	30.0%
	TTCAACAATCTTGAGAATCA+AGG	+	chr5_3:68583950-68583969	None:intergenic	30.0%
	AAGCCTGCAAAATATAGAAA+GGG	+	chr5_3:68584074-68584093	None:intergenic	30.0%
	AAAGCCTGCAAAATATAGAA+AGG	+	chr5_3:68584075-68584094	None:intergenic	30.0%
!!!	GTCATCATGTTTTGAAACTT+TGG	+	chr5_3:68584182-68584201	None:intergenic	30.0%
!	CTAAAATGTTGCAACCTTTT+TGG	-	chr5_3:68584208-68584227	Msa0827420:intron	30.0%
!!!	ACTAGTATTAGCTTTTTTGG+TGG	-	chr5_3:68584265-68584284	Msa0827420:intron	30.0%
!!!	CTAGTATTAGCTTTTTTGGT+GGG	-	chr5_3:68584266-68584285	Msa0827420:intron	30.0%
!!!	TAGTATTAGCTTTTTTGGTG+GGG	-	chr5_3:68584267-68584286	Msa0827420:intron	30.0%
	AGATCAAAATTCTGCTTACT+TGG	-	chr5_3:68584306-68584325	Msa0827420:intron	30.0%
	ATAATTTCAGGAACCAATCA+AGG	-	chr5_3:68584370-68584389	Msa0827420:intron	30.0%
	ACAAATCAATCATTGTTGTG+AGG	+	chr5_3:68584529-68584548	None:intergenic	30.0%
	ACATAATAAGAGCTAGAGTT+TGG	+	chr5_3:68584587-68584606	None:intergenic	30.0%
	AAAACATCTAGACACACAAT+AGG	+	chr5_3:68584682-68584701	None:intergenic	30.0%
	GGGTTTATATTTAGACACAT+AGG	-	chr5_3:68584855-68584874	Msa0827420:intron	30.0%
!	ATTACTGCTATTTGACCATT+TGG	-	chr5_3:68584891-68584910	Msa0827420:intron	30.0%
	GATGACACAACAACAATTAA+TGG	-	chr5_3:68585004-68585023	Msa0827420:intron	30.0%
!!	ACCAGAAGATTTTAGGTAAA+GGG	+	chr5_3:68585306-68585325	None:intergenic	30.0%
	TGGTAAAACTAATTAGTTCC+TGG	-	chr5_3:68585349-68585368	Msa0827420:CDS	30.0%
	ACATTAAGTTAAGTAGCTGA+AGG	-	chr5_3:68585401-68585420	Msa0827420:five_prime_UTR	30.0%
	AGTTACTAGTACACAAACAT+AGG	+	chr5_3:68585508-68585527	None:intergenic	30.0%
!	TATGCTCTTGTGTAAAGTTT+TGG	-	chr5_3:68585541-68585560	Msa0827420:five_prime_UTR	30.0%
!	AGAAGAGTGGTTTTTCTTTG+AGG	+	chr5_3:68583485-68583504	None:intergenic	35.0%
	CACTACTGATCATATTCAAC+AGG	-	chr5_3:68583742-68583761	Msa0827420:CDS	35.0%
	AGGAGAAGAAGAAGAAAGAA+AGG	+	chr5_3:68583770-68583789	None:intergenic	35.0%
!	TGTCCCTTTCTATATTTTGC+AGG	-	chr5_3:68584068-68584087	Msa0827420:CDS	35.0%
!!!	TTTTTCTAAGATGTGGGAGT+GGG	-	chr5_3:68584092-68584111	Msa0827420:CDS	35.0%
!!!	AGTATTAGCTTTTTTGGTGG+GGG	-	chr5_3:68584268-68584287	Msa0827420:intron	35.0%
	AAGAGCTAGAGTTTGGTAAA+TGG	+	chr5_3:68584580-68584599	None:intergenic	35.0%
	ATGTCATTCAGAAGATTGTG+AGG	+	chr5_3:68584917-68584936	None:intergenic	35.0%
	TTCACATGTTGCATAGTGAA+GGG	-	chr5_3:68585120-68585139	Msa0827420:intron	35.0%
	ACCCTTTACCTAAAATCTTC+TGG	-	chr5_3:68585302-68585321	Msa0827420:CDS	35.0%
!!	CACCAGAAGATTTTAGGTAA+AGG	+	chr5_3:68585307-68585326	None:intergenic	35.0%
	TACCTAAAATCTTCTGGTGA+TGG	-	chr5_3:68585308-68585327	Msa0827420:CDS	35.0%
	ACCTAAAATCTTCTGGTGAT+GGG	-	chr5_3:68585309-68585328	Msa0827420:CDS	35.0%
	GGGAATTAGTTAAGCAAGAA+TGG	-	chr5_3:68585329-68585348	Msa0827420:CDS	35.0%
	CCTAATCCTCCTAACTAAAA+AGG	+	chr5_3:68585375-68585394	None:intergenic	35.0%
!!!	CCTTTTTAGTTAGGAGGATT+AGG	-	chr5_3:68585372-68585391	Msa0827420:exon	35.0%
!!	AAAGTTTTGGCCAGAAAATG+AGG	-	chr5_3:68585554-68585573	Msa0827420:five_prime_UTR	35.0%
!	AATTGAGCAACCTCATTTTC+TGG	+	chr5_3:68585567-68585586	None:intergenic	35.0%
	AATGAGGTTGCTCAATTTCA+AGG	-	chr5_3:68585570-68585589	Msa0827420:five_prime_UTR	35.0%
	AAGAGGCAATGGTAGTATTA+TGG	+	chr5_3:68583456-68583475	None:intergenic	35.0%
!	AGAAGAGTGGTTTTTCTTTG+AGG	+	chr5_3:68583485-68583504	None:intergenic	35.0%
	CACTACTGATCATATTCAAC+AGG	-	chr5_3:68583742-68583761	Msa0827420:CDS	35.0%
	AGGAGAAGAAGAAGAAAGAA+AGG	+	chr5_3:68583770-68583789	None:intergenic	35.0%
!	TGTCCCTTTCTATATTTTGC+AGG	-	chr5_3:68584068-68584087	Msa0827420:CDS	35.0%
!!!	TTTTTCTAAGATGTGGGAGT+GGG	-	chr5_3:68584092-68584111	Msa0827420:CDS	35.0%
!!!	AGTATTAGCTTTTTTGGTGG+GGG	-	chr5_3:68584268-68584287	Msa0827420:intron	35.0%
	AAGAGCTAGAGTTTGGTAAA+TGG	+	chr5_3:68584580-68584599	None:intergenic	35.0%
	ATGTCATTCAGAAGATTGTG+AGG	+	chr5_3:68584917-68584936	None:intergenic	35.0%
	TTCACATGTTGCATAGTGAA+GGG	-	chr5_3:68585120-68585139	Msa0827420:intron	35.0%
	ACCCTTTACCTAAAATCTTC+TGG	-	chr5_3:68585302-68585321	Msa0827420:CDS	35.0%
!!	CACCAGAAGATTTTAGGTAA+AGG	+	chr5_3:68585307-68585326	None:intergenic	35.0%
	TACCTAAAATCTTCTGGTGA+TGG	-	chr5_3:68585308-68585327	Msa0827420:CDS	35.0%
	ACCTAAAATCTTCTGGTGAT+GGG	-	chr5_3:68585309-68585328	Msa0827420:CDS	35.0%
	GGGAATTAGTTAAGCAAGAA+TGG	-	chr5_3:68585329-68585348	Msa0827420:CDS	35.0%
	CCTAATCCTCCTAACTAAAA+AGG	+	chr5_3:68585375-68585394	None:intergenic	35.0%
!!!	CCTTTTTAGTTAGGAGGATT+AGG	-	chr5_3:68585372-68585391	Msa0827420:exon	35.0%
!!	AAAGTTTTGGCCAGAAAATG+AGG	-	chr5_3:68585554-68585573	Msa0827420:five_prime_UTR	35.0%
!	AATTGAGCAACCTCATTTTC+TGG	+	chr5_3:68585567-68585586	None:intergenic	35.0%
	AATGAGGTTGCTCAATTTCA+AGG	-	chr5_3:68585570-68585589	Msa0827420:five_prime_UTR	35.0%
!!	AGAGTGGTTTTTCTTTGAGG+CGG	+	chr5_3:68583482-68583501	None:intergenic	40.0%
	CTACACAACACTTAGAAGAG+TGG	+	chr5_3:68583498-68583517	None:intergenic	40.0%
	AACAGAGAAACAGAAGAGTC+TGG	+	chr5_3:68583568-68583587	None:intergenic	40.0%
	GTTTCTTTCTGCTCTTGTTC+AGG	-	chr5_3:68583595-68583614	Msa0827420:three_prime_UTR	40.0%
	TGATCAGTAGTGACGTTGTT+AGG	+	chr5_3:68583734-68583753	None:intergenic	40.0%
!!	AGCAGAGAAAGAGTATCCAA+TGG	+	chr5_3:68583806-68583825	None:intergenic	40.0%
	ATTGTTGAAAGCCAGAACTC+TGG	-	chr5_3:68583961-68583980	Msa0827420:CDS	40.0%
!!!	TGCAGGCTTTTTCTAAGATG+TGG	-	chr5_3:68584085-68584104	Msa0827420:CDS	40.0%
!!!	GCAGGCTTTTTCTAAGATGT+GGG	-	chr5_3:68584086-68584105	Msa0827420:CDS	40.0%
!!!	CTTTTTCTAAGATGTGGGAG+TGG	-	chr5_3:68584091-68584110	Msa0827420:CDS	40.0%
	TCAAGATGCTGTTGCCAAAA+AGG	+	chr5_3:68584225-68584244	None:intergenic	40.0%
!	AAAATAGATAGGGGTGTACC+TGG	+	chr5_3:68584464-68584483	None:intergenic	40.0%
	GTTAGTACAAGTGACCACTT+AGG	-	chr5_3:68584493-68584512	Msa0827420:intron	40.0%
	TACAAGTGACCACTTAGGAA+GGG	-	chr5_3:68584498-68584517	Msa0827420:intron	40.0%
	CTGTTGAGCATACATAAGAG+AGG	+	chr5_3:68585039-68585058	None:intergenic	40.0%
	ATGAATTCGAGTGGAAGTCA+AGG	-	chr5_3:68585095-68585114	Msa0827420:CDS	40.0%
	CTTCACTATGCAACATGTGA+AGG	+	chr5_3:68585121-68585140	None:intergenic	40.0%
	CTTCACATGTTGCATAGTGA+AGG	-	chr5_3:68585119-68585138	Msa0827420:intron	40.0%
	TCACATGTTGCATAGTGAAG+GGG	-	chr5_3:68585121-68585140	Msa0827420:intron	40.0%
!!	AATTCATCGTTAGGTACTGG+AGG	-	chr5_3:68585158-68585177	Msa0827420:intron	40.0%
!	ACTGGAGGTTTTCCTGATTT+TGG	-	chr5_3:68585173-68585192	Msa0827420:intron	40.0%
!	TCCCATCACCAGAAGATTTT+AGG	+	chr5_3:68585313-68585332	None:intergenic	40.0%
!!!	AGTTCCTGGCCTTTTTAGTT+AGG	-	chr5_3:68585363-68585382	Msa0827420:exon	40.0%
!!	AGAGTGGTTTTTCTTTGAGG+CGG	+	chr5_3:68583482-68583501	None:intergenic	40.0%
	CTACACAACACTTAGAAGAG+TGG	+	chr5_3:68583498-68583517	None:intergenic	40.0%
	AACAGAGAAACAGAAGAGTC+TGG	+	chr5_3:68583568-68583587	None:intergenic	40.0%
	GTTTCTTTCTGCTCTTGTTC+AGG	-	chr5_3:68583595-68583614	Msa0827420:three_prime_UTR	40.0%
	TGATCAGTAGTGACGTTGTT+AGG	+	chr5_3:68583734-68583753	None:intergenic	40.0%
!!	AGCAGAGAAAGAGTATCCAA+TGG	+	chr5_3:68583806-68583825	None:intergenic	40.0%
	ATTGTTGAAAGCCAGAACTC+TGG	-	chr5_3:68583961-68583980	Msa0827420:CDS	40.0%
!!!	TGCAGGCTTTTTCTAAGATG+TGG	-	chr5_3:68584085-68584104	Msa0827420:CDS	40.0%
!!!	GCAGGCTTTTTCTAAGATGT+GGG	-	chr5_3:68584086-68584105	Msa0827420:CDS	40.0%
!!!	CTTTTTCTAAGATGTGGGAG+TGG	-	chr5_3:68584091-68584110	Msa0827420:CDS	40.0%
	TCAAGATGCTGTTGCCAAAA+AGG	+	chr5_3:68584225-68584244	None:intergenic	40.0%
!	AAAATAGATAGGGGTGTACC+TGG	+	chr5_3:68584464-68584483	None:intergenic	40.0%
	GTTAGTACAAGTGACCACTT+AGG	-	chr5_3:68584493-68584512	Msa0827420:intron	40.0%
	TACAAGTGACCACTTAGGAA+GGG	-	chr5_3:68584498-68584517	Msa0827420:intron	40.0%
	CTGTTGAGCATACATAAGAG+AGG	+	chr5_3:68585039-68585058	None:intergenic	40.0%
	ATGAATTCGAGTGGAAGTCA+AGG	-	chr5_3:68585095-68585114	Msa0827420:CDS	40.0%
	CTTCACTATGCAACATGTGA+AGG	+	chr5_3:68585121-68585140	None:intergenic	40.0%
	CTTCACATGTTGCATAGTGA+AGG	-	chr5_3:68585119-68585138	Msa0827420:intron	40.0%
	TCACATGTTGCATAGTGAAG+GGG	-	chr5_3:68585121-68585140	Msa0827420:intron	40.0%
!!	AATTCATCGTTAGGTACTGG+AGG	-	chr5_3:68585158-68585177	Msa0827420:intron	40.0%
!	ACTGGAGGTTTTCCTGATTT+TGG	-	chr5_3:68585173-68585192	Msa0827420:intron	40.0%
!	TCCCATCACCAGAAGATTTT+AGG	+	chr5_3:68585313-68585332	None:intergenic	40.0%
!!!	AGTTCCTGGCCTTTTTAGTT+AGG	-	chr5_3:68585363-68585382	Msa0827420:exon	40.0%
!!	TTTCTTTGAGGCGGCTAAAG+AGG	+	chr5_3:68583473-68583492	None:intergenic	45.0%
	GGATAGTAAGCTGCCATCAT+GGG	+	chr5_3:68583713-68583732	None:intergenic	45.0%
	AGGATAGTAAGCTGCCATCA+TGG	+	chr5_3:68583714-68583733	None:intergenic	45.0%
	CTGTTCTTACTCAGCACACT+CGG	+	chr5_3:68583995-68584014	None:intergenic	45.0%
!!!	ATTAGCTTTTTTGGTGGGGG+AGG	-	chr5_3:68584271-68584290	Msa0827420:intron	45.0%
!!!	TTAGCTTTTTTGGTGGGGGA+GGG	-	chr5_3:68584272-68584291	Msa0827420:intron	45.0%
	GATTTCTCTGCAGCCTTGAT+TGG	+	chr5_3:68584386-68584405	None:intergenic	45.0%
	GAGAATCAGCTATTGCAGCT+AGG	+	chr5_3:68584416-68584435	None:intergenic	45.0%
	GTACAAGTGACCACTTAGGA+AGG	-	chr5_3:68584497-68584516	Msa0827420:intron	45.0%
	ACAAGTGACCACTTAGGAAG+GGG	-	chr5_3:68584499-68584518	Msa0827420:intron	45.0%
	CAGAAGATTGTGAGGCCAAA+TGG	+	chr5_3:68584909-68584928	None:intergenic	45.0%
	ACATTGCAGTACCCTTCAAG+TGG	-	chr5_3:68584933-68584952	Msa0827420:intron	45.0%
	GCTGCATCATTCCACTTGAA+GGG	+	chr5_3:68584947-68584966	None:intergenic	45.0%
	TGCTGCATCATTCCACTTGA+AGG	+	chr5_3:68584948-68584967	None:intergenic	45.0%
	TTCAAGTGGAATGATGCAGC+AGG	-	chr5_3:68584947-68584966	Msa0827420:intron	45.0%
	TCAAGTGGAATGATGCAGCA+GGG	-	chr5_3:68584948-68584967	Msa0827420:intron	45.0%
	CACTCGAATTCATACCGAGT+TGG	+	chr5_3:68585088-68585107	None:intergenic	45.0%
	CAACTCGGTATGAATTCGAG+TGG	-	chr5_3:68585086-68585105	Msa0827420:CDS	45.0%
	GTTGGAGGCAATTCATCGTT+AGG	-	chr5_3:68585149-68585168	Msa0827420:intron	45.0%
	GGCAATTCATCGTTAGGTAC+TGG	-	chr5_3:68585155-68585174	Msa0827420:intron	45.0%
!!	TCCTGATTTTGGTCGTAGCT+CGG	-	chr5_3:68585184-68585203	Msa0827420:intron	45.0%
!!	CAAGACATTGGAAGTGCTGA+TGG	-	chr5_3:68585236-68585255	Msa0827420:intron	45.0%
!!!	TCCTGGCCTTTTTAGTTAGG+AGG	-	chr5_3:68585366-68585385	Msa0827420:exon	45.0%
	TCCTCCTAACTAAAAAGGCC+AGG	+	chr5_3:68585370-68585389	None:intergenic	45.0%
!!	TTTCTTTGAGGCGGCTAAAG+AGG	+	chr5_3:68583473-68583492	None:intergenic	45.0%
	GGATAGTAAGCTGCCATCAT+GGG	+	chr5_3:68583713-68583732	None:intergenic	45.0%
	AGGATAGTAAGCTGCCATCA+TGG	+	chr5_3:68583714-68583733	None:intergenic	45.0%
	CTGTTCTTACTCAGCACACT+CGG	+	chr5_3:68583995-68584014	None:intergenic	45.0%
!!!	ATTAGCTTTTTTGGTGGGGG+AGG	-	chr5_3:68584271-68584290	Msa0827420:intron	45.0%
!!!	TTAGCTTTTTTGGTGGGGGA+GGG	-	chr5_3:68584272-68584291	Msa0827420:intron	45.0%
	GATTTCTCTGCAGCCTTGAT+TGG	+	chr5_3:68584386-68584405	None:intergenic	45.0%
	GAGAATCAGCTATTGCAGCT+AGG	+	chr5_3:68584416-68584435	None:intergenic	45.0%
	GTACAAGTGACCACTTAGGA+AGG	-	chr5_3:68584497-68584516	Msa0827420:intron	45.0%
	ACAAGTGACCACTTAGGAAG+GGG	-	chr5_3:68584499-68584518	Msa0827420:intron	45.0%
	CAGAAGATTGTGAGGCCAAA+TGG	+	chr5_3:68584909-68584928	None:intergenic	45.0%
	ACATTGCAGTACCCTTCAAG+TGG	-	chr5_3:68584933-68584952	Msa0827420:intron	45.0%
	GCTGCATCATTCCACTTGAA+GGG	+	chr5_3:68584947-68584966	None:intergenic	45.0%
	TGCTGCATCATTCCACTTGA+AGG	+	chr5_3:68584948-68584967	None:intergenic	45.0%
	TTCAAGTGGAATGATGCAGC+AGG	-	chr5_3:68584947-68584966	Msa0827420:intron	45.0%
	TCAAGTGGAATGATGCAGCA+GGG	-	chr5_3:68584948-68584967	Msa0827420:intron	45.0%
	CACTCGAATTCATACCGAGT+TGG	+	chr5_3:68585088-68585107	None:intergenic	45.0%
	CAACTCGGTATGAATTCGAG+TGG	-	chr5_3:68585086-68585105	Msa0827420:CDS	45.0%
	GTTGGAGGCAATTCATCGTT+AGG	-	chr5_3:68585149-68585168	Msa0827420:intron	45.0%
	GGCAATTCATCGTTAGGTAC+TGG	-	chr5_3:68585155-68585174	Msa0827420:intron	45.0%
!!	TCCTGATTTTGGTCGTAGCT+CGG	-	chr5_3:68585184-68585203	Msa0827420:intron	45.0%
!!	CAAGACATTGGAAGTGCTGA+TGG	-	chr5_3:68585236-68585255	Msa0827420:intron	45.0%
!!!	TCCTGGCCTTTTTAGTTAGG+AGG	-	chr5_3:68585366-68585385	Msa0827420:exon	45.0%
	TCCTCCTAACTAAAAAGGCC+AGG	+	chr5_3:68585370-68585389	None:intergenic	45.0%
!!	AAAATAAATAGTTAAAAAAA+GGG	+	chr5_3:68583886-68583905	None:intergenic	5.0%
!!!	AAAAAAACTTTATTTTATTA+AGG	+	chr5_3:68584134-68584153	None:intergenic	5.0%
!!!	TTTGAAATAAAATTTATTTT+TGG	-	chr5_3:68584810-68584829	Msa0827420:intron	5.0%
!!	AAAATAAATAGTTAAAAAAA+GGG	+	chr5_3:68583886-68583905	None:intergenic	5.0%
!!!	AAAAAAACTTTATTTTATTA+AGG	+	chr5_3:68584134-68584153	None:intergenic	5.0%
!!!	TTTGAAATAAAATTTATTTT+TGG	-	chr5_3:68584810-68584829	Msa0827420:intron	5.0%
	TCTGCTCTTGTTCAGGTCAC+AGG	-	chr5_3:68583602-68583621	Msa0827420:three_prime_UTR	50.0%
	CTGCTCTTGTTCAGGTCACA+GGG	-	chr5_3:68583603-68583622	Msa0827420:three_prime_UTR	50.0%
	CAGGGTCACACACCTTTGTT+TGG	-	chr5_3:68583621-68583640	Msa0827420:three_prime_UTR	50.0%
	CAAGCCTTGCAAAGCCAACT+CGG	-	chr5_3:68585071-68585090	Msa0827420:CDS	50.0%
!!	CATACCGAGTTGGCTTTGCA+AGG	+	chr5_3:68585078-68585097	None:intergenic	50.0%
	CATAGTGAAGGGGCTAACGT+TGG	-	chr5_3:68585131-68585150	Msa0827420:intron	50.0%
!	GGTAGTGGCAAGCAAGACAT+TGG	-	chr5_3:68585224-68585243	Msa0827420:intron	50.0%
	TCTAGTGGTCACTCTGGTGA+TGG	-	chr5_3:68585272-68585291	Msa0827420:intron	50.0%
	TCTGCTCTTGTTCAGGTCAC+AGG	-	chr5_3:68583602-68583621	Msa0827420:three_prime_UTR	50.0%
	CTGCTCTTGTTCAGGTCACA+GGG	-	chr5_3:68583603-68583622	Msa0827420:three_prime_UTR	50.0%
	CAGGGTCACACACCTTTGTT+TGG	-	chr5_3:68583621-68583640	Msa0827420:three_prime_UTR	50.0%
	CAAGCCTTGCAAAGCCAACT+CGG	-	chr5_3:68585071-68585090	Msa0827420:CDS	50.0%
!!	CATACCGAGTTGGCTTTGCA+AGG	+	chr5_3:68585078-68585097	None:intergenic	50.0%
	CATAGTGAAGGGGCTAACGT+TGG	-	chr5_3:68585131-68585150	Msa0827420:intron	50.0%
!	GGTAGTGGCAAGCAAGACAT+TGG	-	chr5_3:68585224-68585243	Msa0827420:intron	50.0%
	TCTAGTGGTCACTCTGGTGA+TGG	-	chr5_3:68585272-68585291	Msa0827420:intron	50.0%
	TGAGGCGGCTAAAGAGGCAA+TGG	+	chr5_3:68583467-68583486	None:intergenic	55.0%
!	TCATGGGCTGCATCTGCATC+AGG	+	chr5_3:68583697-68583716	None:intergenic	55.0%
	GATGCAGATGCAGCCCATGA+TGG	-	chr5_3:68583697-68583716	Msa0827420:three_prime_UTR	55.0%
	CCAGGCATAGTAGGTGGTTG+GGG	+	chr5_3:68584441-68584460	None:intergenic	55.0%
	GCCAGGCATAGTAGGTGGTT+GGG	+	chr5_3:68584442-68584461	None:intergenic	55.0%
	TGTACCTGGCCAGGCATAGT+AGG	+	chr5_3:68584450-68584469	None:intergenic	55.0%
	GAGGTGAACCCCTTCCTAAG+TGG	+	chr5_3:68584510-68584529	None:intergenic	55.0%
	AGTGGAATGATGCAGCAGGG+AGG	-	chr5_3:68584951-68584970	Msa0827420:intron	55.0%
	AGTGAAGGGGCTAACGTTGG+AGG	-	chr5_3:68585134-68585153	Msa0827420:intron	55.0%
	GCCGAGCTACGACCAAAATC+AGG	+	chr5_3:68585188-68585207	None:intergenic	55.0%
!!	GATTTTGGTCGTAGCTCGGC+TGG	-	chr5_3:68585188-68585207	Msa0827420:intron	55.0%
!!	TCGGCTGGTGATGGTTTGCA+CGG	-	chr5_3:68585203-68585222	Msa0827420:intron	55.0%
!	GGTGATGGTTTGCACGGTAG+TGG	-	chr5_3:68585209-68585228	Msa0827420:intron	55.0%
!!	ATTGGAAGTGCTGATGGCCG+CGG	-	chr5_3:68585242-68585261	Msa0827420:intron	55.0%
!	GGAAGCTCTAGTGGTCACTC+TGG	-	chr5_3:68585266-68585285	Msa0827420:intron	55.0%
!!	AGTGGTCACTCTGGTGATGG+CGG	-	chr5_3:68585275-68585294	Msa0827420:intron	55.0%
	TGAGGCGGCTAAAGAGGCAA+TGG	+	chr5_3:68583467-68583486	None:intergenic	55.0%
!	TCATGGGCTGCATCTGCATC+AGG	+	chr5_3:68583697-68583716	None:intergenic	55.0%
	GATGCAGATGCAGCCCATGA+TGG	-	chr5_3:68583697-68583716	Msa0827420:three_prime_UTR	55.0%
	CCAGGCATAGTAGGTGGTTG+GGG	+	chr5_3:68584441-68584460	None:intergenic	55.0%
	GCCAGGCATAGTAGGTGGTT+GGG	+	chr5_3:68584442-68584461	None:intergenic	55.0%
	TGTACCTGGCCAGGCATAGT+AGG	+	chr5_3:68584450-68584469	None:intergenic	55.0%
	GAGGTGAACCCCTTCCTAAG+TGG	+	chr5_3:68584510-68584529	None:intergenic	55.0%
	AGTGGAATGATGCAGCAGGG+AGG	-	chr5_3:68584951-68584970	Msa0827420:intron	55.0%
	AGTGAAGGGGCTAACGTTGG+AGG	-	chr5_3:68585134-68585153	Msa0827420:intron	55.0%
	GCCGAGCTACGACCAAAATC+AGG	+	chr5_3:68585188-68585207	None:intergenic	55.0%
!!	GATTTTGGTCGTAGCTCGGC+TGG	-	chr5_3:68585188-68585207	Msa0827420:intron	55.0%
!!	TCGGCTGGTGATGGTTTGCA+CGG	-	chr5_3:68585203-68585222	Msa0827420:intron	55.0%
!	GGTGATGGTTTGCACGGTAG+TGG	-	chr5_3:68585209-68585228	Msa0827420:intron	55.0%
!!	ATTGGAAGTGCTGATGGCCG+CGG	-	chr5_3:68585242-68585261	Msa0827420:intron	55.0%
!	GGAAGCTCTAGTGGTCACTC+TGG	-	chr5_3:68585266-68585285	Msa0827420:intron	55.0%
!!	AGTGGTCACTCTGGTGATGG+CGG	-	chr5_3:68585275-68585294	Msa0827420:intron	55.0%
	CGGTGAGCTTGCCAGAGTTC+TGG	+	chr5_3:68583975-68583994	None:intergenic	60.0%
!!!	GCTTTTTTGGTGGGGGAGGG+AGG	-	chr5_3:68584275-68584294	Msa0827420:intron	60.0%
	CCCCAACCACCTACTATGCC+TGG	-	chr5_3:68584438-68584457	Msa0827420:intron	60.0%
	GGCCAGGCATAGTAGGTGGT+TGG	+	chr5_3:68584443-68584462	None:intergenic	60.0%
	ACCACCTACTATGCCTGGCC+AGG	-	chr5_3:68584443-68584462	Msa0827420:intron	60.0%
	ACCTGGCCAGGCATAGTAGG+TGG	+	chr5_3:68584447-68584466	None:intergenic	60.0%
!	AGATAGGGGTGTACCTGGCC+AGG	+	chr5_3:68584459-68584478	None:intergenic	60.0%
	GCAGGGAGGACACTACATGC+AGG	-	chr5_3:68584965-68584984	Msa0827420:intron	60.0%
	GACCACTAGAGCTTCCACCG+CGG	+	chr5_3:68585262-68585281	None:intergenic	60.0%
	CGGTGAGCTTGCCAGAGTTC+TGG	+	chr5_3:68583975-68583994	None:intergenic	60.0%
!!!	GCTTTTTTGGTGGGGGAGGG+AGG	-	chr5_3:68584275-68584294	Msa0827420:intron	60.0%
	CCCCAACCACCTACTATGCC+TGG	-	chr5_3:68584438-68584457	Msa0827420:intron	60.0%
	GGCCAGGCATAGTAGGTGGT+TGG	+	chr5_3:68584443-68584462	None:intergenic	60.0%
	ACCACCTACTATGCCTGGCC+AGG	-	chr5_3:68584443-68584462	Msa0827420:intron	60.0%
	ACCTGGCCAGGCATAGTAGG+TGG	+	chr5_3:68584447-68584466	None:intergenic	60.0%
!	AGATAGGGGTGTACCTGGCC+AGG	+	chr5_3:68584459-68584478	None:intergenic	60.0%
	GCAGGGAGGACACTACATGC+AGG	-	chr5_3:68584965-68584984	Msa0827420:intron	60.0%
	GACCACTAGAGCTTCCACCG+CGG	+	chr5_3:68585262-68585281	None:intergenic	60.0%
	GGTCGTAGCTCGGCTGGTGA+TGG	-	chr5_3:68585194-68585213	Msa0827420:intron	65.0%
	GGTCGTAGCTCGGCTGGTGA+TGG	-	chr5_3:68585194-68585213	Msa0827420:intron	65.0%
!!	GGAAGTGCTGATGGCCGCGG+TGG	-	chr5_3:68585245-68585264	Msa0827420:intron	70.0%
!	GGCCGCGGTGGAAGCTCTAG+TGG	-	chr5_3:68585257-68585276	Msa0827420:intron	70.0%
!!	GGAAGTGCTGATGGCCGCGG+TGG	-	chr5_3:68585245-68585264	Msa0827420:intron	70.0%
!	GGCCGCGGTGGAAGCTCTAG+TGG	-	chr5_3:68585257-68585276	Msa0827420:intron	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr5_3	gene	68583454	68585607	68583454	ID=Msa0827420;Name=Msa0827420
chr5_3	mRNA	68583454	68585607	68583454	ID=Msa0827420-mRNA-1;Parent=Msa0827420;Name=Msa0827420-mRNA-1;_AED=0.01;_eAED=0.01;_QI=229\|0.66\|0.75\|1\|1\|1\|4\|270\|213
chr5_3	exon	68583454	68584119	68583454	ID=Msa0827420-mRNA-1:exon:21515;Parent=Msa0827420-mRNA-1
chr5_3	exon	68584597	68584681	68584597	ID=Msa0827420-mRNA-1:exon:21514;Parent=Msa0827420-mRNA-1
chr5_3	exon	68585057	68585136	68585057	ID=Msa0827420-mRNA-1:exon:21513;Parent=Msa0827420-mRNA-1
chr5_3	exon	68585298	68585607	68585298	ID=Msa0827420-mRNA-1:exon:21512;Parent=Msa0827420-mRNA-1
chr5_3	five_prime_UTR	68585379	68585607	68585379	ID=Msa0827420-mRNA-1:five_prime_utr;Parent=Msa0827420-mRNA-1
chr5_3	CDS	68585298	68585378	68585298	ID=Msa0827420-mRNA-1:cds;Parent=Msa0827420-mRNA-1
chr5_3	CDS	68585057	68585136	68585057	ID=Msa0827420-mRNA-1:cds;Parent=Msa0827420-mRNA-1
chr5_3	CDS	68584597	68584681	68584597	ID=Msa0827420-mRNA-1:cds;Parent=Msa0827420-mRNA-1
chr5_3	CDS	68583724	68584119	68583724	ID=Msa0827420-mRNA-1:cds;Parent=Msa0827420-mRNA-1
chr5_3	three_prime_UTR	68583454	68583723	68583454	ID=Msa0827420-mRNA-1:three_prime_utr;Parent=Msa0827420-mRNA-1

Gene Sequence

>Msa0827420

ATGCAGCAGCACCTGATGCAGATGCAGCCCATGATGGCAGCTTACTATCCTAACAACGTCACTACTGATCATATTCAACAGCAGTATCTTGATGAGAACAAGTCCTTGATTCTCAAGATTGTTGAAAGCCAGAACTCTGGCAAGCTCACCGAGTGTGCTGAGAACCAATCAAGGCTGCAGAGAAATCTCATGTACCTAGCTGCAATAGCTGATTCTCAACCCCAACCACCTACTATGCCTGGCCAGTACCCTTCAAGTGGAATGATGCAGCAGGGAGGACACTACATGCAGGCTCAACAAGCTCAGCAGATGACACAACAACAATTAATGGCTGCACGTTCCTCTCTTATGTATGCTCAACAGCTTCAACAGCAGCAAGCCTTGCAAAGCCAACTCGGTATGAATTCGAGTGGAAGTCAAGGCCTTCACATGTTGCATAGTGAAGGGGCTAACGTTGGAGGCAATTCATCGTTAGGTACTGGAGGTTTTCCTGATTTTGGTCGTAGCTCGGCTGGTGATGGTTTGCACGGTAGTGGCAAGCAAGACATTGGAAGTGCTGATGGCCGCGGTGGAAGCTCTAGTGGTCACTCTGGTGATGGCGGCGAAACCCTTTACCTAAAATCTTCTGGTGATGGGAATTAG

Protein sequence

>Msa0827420

MQQHLMQMQPMMAAYYPNNVTTDHIQQQYLDENKSLILKIVESQNSGKLTECAENQSRLQRNLMYLAAIADSQPQPPTMPGQYPSSGMMQQGGHYMQAQQAQQMTQQQLMAARSSLMYAQQLQQQQALQSQLGMNSSGSQGLHMLHSEGANVGGNSSLGTGGFPDFGRSSAGDGLHGSGKQDIGSADGRGGSSSGHSGDGGETLYLKSSGDGN*