AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1404460

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1404460 MtrunA17_Chr1g0209281 98.964 193 2 0 1 193 1 193 2.13e-141 391
Msa1404460 MtrunA17_Chr1g0209321 46.114 193 91 4 1 191 1 182 1.37e-48 156
Msa1404460 MtrunA17_Chr2g0318011 48.503 167 86 0 4 170 3 169 1.54e-46 151
Msa1404460 MtrunA17_Chr5g0423771 41.379 174 102 0 4 177 7 180 4.86e-45 147
Msa1404460 MtrunA17_Chr4g0047771 43.787 169 93 2 1 169 1 167 3.62e-42 140
Msa1404460 MtrunA17_Chr1g0209301 67.416 89 28 1 102 189 17 105 1.72e-35 120
Msa1404460 MtrunA17_Chr1g0209311 55.000 100 43 1 1 100 1 98 3.33e-33 114
Msa1404460 MtrunA17_Chr1g0209291 52.000 100 46 1 1 100 1 98 5.64e-27 98.6
Msa1404460 MtrunA17_Chr4g0039411 30.978 184 117 6 1 178 1 180 1.14e-13 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1404460 AT1G68220.1 62.564 195 70 2 1 192 1 195 1.91e-85 251
Msa1404460 AT1G52910.1 46.286 175 92 2 1 174 1 174 3.00e-49 158
Msa1404460 AT3G15480.1 47.753 178 87 3 1 177 1 173 1.32e-48 156
Msa1404460 AT4G27435.1 49.045 157 80 0 10 166 9 165 3.08e-47 153
Msa1404460 AT1G13380.1 45.399 163 86 1 4 166 7 166 8.83e-42 139
Msa1404460 AT1G61065.1 41.916 167 94 2 17 183 16 179 6.14e-39 132
Msa1404460 AT5G17210.1 32.215 149 97 3 14 160 15 161 8.20e-13 64.7

Find 77 sgRNAs with CRISPR-Local

Find 178 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAGGCTGACACTGGTTTC+TGG 0.168217 tig0023067:+21071 Msa1404460:CDS
TGTAAAGTGACCATTCTTTC+TGG 0.168738 tig0023067:-21529 None:intergenic
TTTAATATTTATGCCGTAAT+TGG 0.187655 tig0023067:+21290 Msa1404460:three_prime_UTR
AAATTCAAATTTCAAGATAT+TGG 0.187944 tig0023067:-21686 None:intergenic
CCAATCCAATGCCTTCATTT+TGG 0.194665 tig0023067:-21102 None:intergenic
TTACCAAAATGAAGGTGTTT+TGG 0.247519 tig0023067:+21219 Msa1404460:three_prime_UTR
CGCAAAGGTGTGTTTGCTGC+TGG 0.268553 tig0023067:+20992 Msa1404460:CDS
CTTCAGGGCTTATCAGATTC+AGG 0.297517 tig0023067:-21142 None:intergenic
AAGTGGAAAACTTGACTAAA+AGG 0.331366 tig0023067:-21505 None:intergenic
TTTCAAGCATTCACCAATTA+CGG 0.344003 tig0023067:-21303 None:intergenic
CAATAGCAACACTTTCAAAC+TGG 0.362205 tig0023067:-21383 None:intergenic
GTTGAAAGCAGTAAACCTTC+AGG 0.380221 tig0023067:-21158 None:intergenic
CTTGACCTTGTTCTCTATGT+TGG 0.382330 tig0023067:+21021 Msa1404460:CDS
TACAGACACAGCCATGATTT+CGG 0.392499 tig0023067:-19398 None:intergenic
GCAGAGGCGTGCTTGTTAGC+AGG 0.394015 tig0023067:+20896 Msa1404460:CDS
AACAAACAATACAGAGTAAA+GGG 0.402361 tig0023067:+19343 Msa1404460:five_prime_UTR
GCAAAGGTGTGTTTGCTGCT+GGG 0.415418 tig0023067:+20993 Msa1404460:CDS
CAAGGCTGACACTGGTTTCT+GGG 0.415808 tig0023067:+21072 Msa1404460:CDS
TGACCATTCTTTCTGGAAAG+TGG 0.424788 tig0023067:-21522 None:intergenic
TTTCCACTTTCCAGAAAGAA+TGG 0.426874 tig0023067:+21519 Msa1404460:three_prime_UTR
AATATCTTCAATTCAATGTC+TGG 0.429834 tig0023067:-19293 None:intergenic
GAACAGTTTGACTGAGAAGC+AGG 0.443123 tig0023067:-19667 None:intergenic
ACAATATATAGATAAATAGA+TGG 0.446395 tig0023067:+21325 Msa1404460:three_prime_UTR
GTTTACTGCTTTCAACATGC+TGG 0.447801 tig0023067:+21165 Msa1404460:three_prime_UTR
CAAATTGAACACCGAAATCA+TGG 0.450311 tig0023067:+19387 Msa1404460:exon
CAAAGGTGTGTTTGCTGCTG+GGG 0.452357 tig0023067:+20994 Msa1404460:CDS
AATAGATGGTAGTGGTATGG+TGG 0.454376 tig0023067:+21339 Msa1404460:three_prime_UTR
ATTAGATGCACTAAAGCAAA+TGG 0.470320 tig0023067:-21426 None:intergenic
CAATGTGTTCAAAACCTTGT+TGG 0.472667 tig0023067:+21258 Msa1404460:three_prime_UTR
AGTGTTGCTATTGAAACAAA+TGG 0.479016 tig0023067:+21393 Msa1404460:three_prime_UTR
TCATCTTCTTCATTCTCTCA+TGG 0.481945 tig0023067:+19759 Msa1404460:CDS
AGAGATGAAGAGAAATGATA+AGG 0.482501 tig0023067:-19432 None:intergenic
GTTCGGTCATCGTACTCATC+AGG 0.486965 tig0023067:-19587 None:intergenic
AGATGTCTTTGCTGTGGTAA+AGG 0.489539 tig0023067:+19704 Msa1404460:CDS
AAACAAACAATACAGAGTAA+AGG 0.496962 tig0023067:+19342 Msa1404460:five_prime_UTR
TGTGGTAAAGGTCTCATCTC+TGG 0.498793 tig0023067:+19716 Msa1404460:CDS
TTGAAAGCAGTAAACCTTCA+GGG 0.502427 tig0023067:-21157 None:intergenic
ATAGATGGTAGTGGTATGGT+GGG 0.504617 tig0023067:+21340 Msa1404460:three_prime_UTR
GCTTGTTAGCAGGCTCTGCT+AGG 0.513926 tig0023067:+20906 Msa1404460:CDS
AAGCACCAAAATGAAGGCAT+TGG 0.516097 tig0023067:+21097 Msa1404460:CDS
TTTACTGCTTTCAACATGCT+GGG 0.519387 tig0023067:+21166 Msa1404460:three_prime_UTR
TGGTGTAAACACAGAAAGTT+CGG 0.520390 tig0023067:-19604 None:intergenic
ATAAATAGATGGTAGTGGTA+TGG 0.540818 tig0023067:+21336 Msa1404460:three_prime_UTR
TGGGAGAAGCACCAAAATGA+AGG 0.543939 tig0023067:+21091 Msa1404460:CDS
CCAAAATGAAGGCATTGGAT+TGG 0.547050 tig0023067:+21102 Msa1404460:CDS
CCCTCGGTACTTTGTGTGGT+AGG 0.550996 tig0023067:-20933 None:intergenic
CCAGAAACCAGTGTCAGCCT+TGG 0.551041 tig0023067:-21071 None:intergenic
GATGCACTAAAGCAAATGGT+TGG 0.551676 tig0023067:-21422 None:intergenic
AATTCCCTCGGTACTTTGTG+TGG 0.559584 tig0023067:-20937 None:intergenic
TATAGATAAATAGATGGTAG+TGG 0.562653 tig0023067:+21331 Msa1404460:three_prime_UTR
GCACACGCCAAGGCTGACAC+TGG 0.573418 tig0023067:+21064 Msa1404460:CDS
AGATGGTAGTGGTATGGTGG+GGG 0.575873 tig0023067:+21342 Msa1404460:three_prime_UTR
ACCGACGCTTCCACTGTCTA+CGG 0.578522 tig0023067:+19626 Msa1404460:CDS
CTGAATCTGATAAGCCCTGA+AGG 0.582169 tig0023067:+21143 Msa1404460:CDS
GTACTATTGGGCACACGCCA+AGG 0.584073 tig0023067:+21054 Msa1404460:CDS
CGCTGAACGACGTCGTAGTG+AGG 0.588172 tig0023067:+19477 Msa1404460:CDS
GAAGAAGATGACAGCACAAG+TGG 0.589788 tig0023067:-19747 None:intergenic
TCGTTCATTATCTTGTTGTG+TGG 0.591988 tig0023067:+21628 Msa1404460:three_prime_UTR
AGCAGCAACACCGTAGACAG+TGG 0.594874 tig0023067:-19636 None:intergenic
GTACTCATCAGGAACCACTT+TGG 0.600621 tig0023067:-19576 None:intergenic
ACCGTAGACAGTGGAAGCGT+CGG 0.606731 tig0023067:-19627 None:intergenic
AGAAGCCAACATAGAGAACA+AGG 0.611678 tig0023067:-21026 None:intergenic
GTCACTAGATGTCTTTGCTG+TGG 0.617569 tig0023067:+19698 Msa1404460:CDS
TAGATGGTAGTGGTATGGTG+GGG 0.627375 tig0023067:+21341 Msa1404460:three_prime_UTR
GTAGACAGTGGAAGCGTCGG+TGG 0.627400 tig0023067:-19624 None:intergenic
CGTGTGTCACAAAATTCCCT+CGG 0.630799 tig0023067:-20949 None:intergenic
CAACATAGAGAACAAGGTCA+AGG 0.636116 tig0023067:-21020 None:intergenic
TCTTGCGCTACACTTCGCAA+AGG 0.645552 tig0023067:+20977 Msa1404460:CDS
GGATTGGCCACACACAATCA+CGG 0.646265 tig0023067:+21118 Msa1404460:CDS
TTCAGGACCGTGATTGTGTG+TGG 0.660100 tig0023067:-21125 None:intergenic
GCCTACCACACAAAGTACCG+AGG 0.682833 tig0023067:+20932 Msa1404460:CDS
CCTACCACACAAAGTACCGA+GGG 0.685131 tig0023067:+20933 Msa1404460:CDS
ACAGCAAAGACATCTAGTGA+CGG 0.697347 tig0023067:-19696 None:intergenic
CACAACAAGATAATGAACGA+GGG 0.700896 tig0023067:-21625 None:intergenic
TTAAATATACTCTACCAACA+AGG 0.709057 tig0023067:-21272 None:intergenic
ACACAACAAGATAATGAACG+AGG 0.713523 tig0023067:-21626 None:intergenic
AACAAGATAATGAACGAGGG+CGG 0.719134 tig0023067:-21622 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAAAAGGGTTTTTTAA+GGG - tig0023067:19817-19836 None:intergenic 15.0%
!!! AAAAAAAAAAGGGTTTTTTA+AGG - tig0023067:19818-19837 None:intergenic 15.0%
!! AAAATTAATTGTGTTGAATT+TGG + tig0023067:19938-19957 Msa1404460:intron 15.0%
!! TTGGTTATTTGATAAAAATT+TGG + tig0023067:19957-19976 Msa1404460:intron 15.0%
!! AAGCAAAAAATAAATATGAT+GGG - tig0023067:20571-20590 None:intergenic 15.0%
!! AAAGCAAAAAATAAATATGA+TGG - tig0023067:20572-20591 None:intergenic 15.0%
!! ACAATATATAGATAAATAGA+TGG + tig0023067:21325-21344 Msa1404460:three_prime_UTR 15.0%
!!! TTACCAAATTTTTTTTATCA+CGG - tig0023067:21462-21481 None:intergenic 15.0%
!! AAATTCAAATTTCAAGATAT+TGG - tig0023067:21689-21708 None:intergenic 15.0%
!!! TTAGTGTTGAAATTAAAGAA+AGG - tig0023067:19513-19532 None:intergenic 20.0%
!!! AAAAAAAAGGGTTTTTTAAG+GGG - tig0023067:19816-19835 None:intergenic 20.0%
!! CTAAGTAATAATACAAAAGA+AGG - tig0023067:20632-20651 None:intergenic 20.0%
!! CTTATACATATAAACACAAA+AGG - tig0023067:20704-20723 None:intergenic 20.0%
!! TTTAATATTTATGCCGTAAT+TGG + tig0023067:21290-21309 Msa1404460:three_prime_UTR 20.0%
!! TTACCGTGATAAAAAAAATT+TGG + tig0023067:21456-21475 Msa1404460:three_prime_UTR 20.0%
! AATATCTTCAATTCAATGTC+TGG - tig0023067:19296-19315 None:intergenic 25.0%
! AAACAAACAATACAGAGTAA+AGG + tig0023067:19342-19361 Msa1404460:five_prime_UTR 25.0%
! AACAAACAATACAGAGTAAA+GGG + tig0023067:19343-19362 Msa1404460:five_prime_UTR 25.0%
!! AATCTGATTAATCTCTTTTC+AGG + tig0023067:19553-19572 Msa1404460:intron 25.0%
! AAATAAAAAGCTGTGAAGAT+TGG - tig0023067:19792-19811 None:intergenic 25.0%
! TCATGGTCTCAAATTATTAA+TGG - tig0023067:20303-20322 None:intergenic 25.0%
! TCTCATAATGTTACAAAAAG+AGG - tig0023067:20467-20486 None:intergenic 25.0%
! TTTCACTAATTAGTAGAACT+AGG + tig0023067:20504-20523 Msa1404460:intron 25.0%
! TTCACTAATTAGTAGAACTA+GGG + tig0023067:20505-20524 Msa1404460:intron 25.0%
!!! TAGACTTCTATATGTTTTTG+AGG + tig0023067:20591-20610 Msa1404460:intron 25.0%
! TTAGTTTACTAGTGATTTAG+TGG + tig0023067:20648-20667 Msa1404460:intron 25.0%
! AATCATCATTATTTCACTTC+AGG - tig0023067:20835-20854 None:intergenic 25.0%
! ATTATTGTTGCTGTAAAATC+AGG + tig0023067:20858-20877 Msa1404460:intron 25.0%
!!! ATTGCTTTTTACCAAAATGA+AGG + tig0023067:21211-21230 Msa1404460:three_prime_UTR 25.0%
! TTAAATATACTCTACCAACA+AGG - tig0023067:21275-21294 None:intergenic 25.0%
! TATAGATAAATAGATGGTAG+TGG + tig0023067:21331-21350 Msa1404460:three_prime_UTR 25.0%
AGAGATGAAGAGAAATGATA+AGG - tig0023067:19435-19454 None:intergenic 30.0%
!! TTAGCTTTCGTTTTTGCTAT+AGG + tig0023067:19454-19473 Msa1404460:CDS 30.0%
GAAGTCTTTGTACATGAATT+CGG + tig0023067:20030-20049 Msa1404460:intron 30.0%
ATGCTGAGCTTGAAAAAATA+TGG - tig0023067:20135-20154 None:intergenic 30.0%
AATTATTAATGGTGTCTACG+TGG - tig0023067:20292-20311 None:intergenic 30.0%
TGGTCATGTGAAAGAAAATA+GGG - tig0023067:20438-20457 None:intergenic 30.0%
ATGGTCATGTGAAAGAAAAT+AGG - tig0023067:20439-20458 None:intergenic 30.0%
GTTACAAAAAGAGGAAGTAA+TGG - tig0023067:20458-20477 None:intergenic 30.0%
TAATTAGTGAAAGATCCTGA+GGG - tig0023067:20496-20515 None:intergenic 30.0%
!! CTATATGTTTTTGAGGATGA+AGG + tig0023067:20598-20617 Msa1404460:intron 30.0%
TCGAGATTCTATGTAAGATT+GGG - tig0023067:20797-20816 None:intergenic 30.0%
! TGGCTTCTATTTTGTACTAT+TGG + tig0023067:21041-21060 Msa1404460:CDS 30.0%
! GGCTTCTATTTTGTACTATT+GGG + tig0023067:21042-21061 Msa1404460:CDS 30.0%
!!! TTACCAAAATGAAGGTGTTT+TGG + tig0023067:21219-21238 Msa1404460:three_prime_UTR 30.0%
!!! TACCAAAATGAAGGTGTTTT+GGG + tig0023067:21220-21239 Msa1404460:three_prime_UTR 30.0%
TTTCAAGCATTCACCAATTA+CGG - tig0023067:21306-21325 None:intergenic 30.0%
!! ATAAATAGATGGTAGTGGTA+TGG + tig0023067:21336-21355 Msa1404460:three_prime_UTR 30.0%
! AGTGTTGCTATTGAAACAAA+TGG + tig0023067:21393-21412 Msa1404460:three_prime_UTR 30.0%
ATTAGATGCACTAAAGCAAA+TGG - tig0023067:21429-21448 None:intergenic 30.0%
AAGTGGAAAACTTGACTAAA+AGG - tig0023067:21508-21527 None:intergenic 30.0%
CAAATTGAACACCGAAATCA+TGG + tig0023067:19387-19406 Msa1404460:exon 35.0%
TGGTGTAAACACAGAAAGTT+CGG - tig0023067:19607-19626 None:intergenic 35.0%
TCATCTTCTTCATTCTCTCA+TGG + tig0023067:19759-19778 Msa1404460:CDS 35.0%
! CGTAAGTGTTGTATATGTGT+TGG + tig0023067:20334-20353 Msa1404460:intron 35.0%
ATGTGAAAGAAAATAGGGGA+AGG - tig0023067:20433-20452 None:intergenic 35.0%
GGTCATGTGAAAGAAAATAG+GGG - tig0023067:20437-20456 None:intergenic 35.0%
AATTAGTGAAAGATCCTGAG+GGG - tig0023067:20495-20514 None:intergenic 35.0%
CTAATTAGTGAAAGATCCTG+AGG - tig0023067:20497-20516 None:intergenic 35.0%
! TAGTAGAACTAGGGTTTTTC+CGG + tig0023067:20514-20533 Msa1404460:intron 35.0%
AACTGTAAGATCCTACAGAA+GGG - tig0023067:20737-20756 None:intergenic 35.0%
ATTCTATGTAAGATTGGGAG+GGG - tig0023067:20792-20811 None:intergenic 35.0%
GATTCTATGTAAGATTGGGA+GGG - tig0023067:20793-20812 None:intergenic 35.0%
AGATTCTATGTAAGATTGGG+AGG - tig0023067:20794-20813 None:intergenic 35.0%
CTCGAGATTCTATGTAAGAT+TGG - tig0023067:20798-20817 None:intergenic 35.0%
!!! TAAAATCAGGACCGGTTTTT+TGG + tig0023067:20871-20890 Msa1404460:intron 35.0%
TTGAAAGCAGTAAACCTTCA+GGG - tig0023067:21160-21179 None:intergenic 35.0%
TTTACTGCTTTCAACATGCT+GGG + tig0023067:21166-21185 Msa1404460:three_prime_UTR 35.0%
!!! ACCAAAATGAAGGTGTTTTG+GGG + tig0023067:21221-21240 Msa1404460:three_prime_UTR 35.0%
CAATGTGTTCAAAACCTTGT+TGG + tig0023067:21258-21277 Msa1404460:three_prime_UTR 35.0%
CAATAGCAACACTTTCAAAC+TGG - tig0023067:21386-21405 None:intergenic 35.0%
TTTCCACTTTCCAGAAAGAA+TGG + tig0023067:21519-21538 Msa1404460:three_prime_UTR 35.0%
TGTAAAGTGACCATTCTTTC+TGG - tig0023067:21532-21551 None:intergenic 35.0%
CCAAAAAAAGATTTGGGAGA+AGG - tig0023067:21600-21619 None:intergenic 35.0%
!!! CCTTCTCCCAAATCTTTTTT+TGG + tig0023067:21597-21616 Msa1404460:three_prime_UTR 35.0%
GCGGTTCCAAAAAAAGATTT+GGG - tig0023067:21606-21625 None:intergenic 35.0%
CACAACAAGATAATGAACGA+GGG - tig0023067:21628-21647 None:intergenic 35.0%
ACACAACAAGATAATGAACG+AGG - tig0023067:21629-21648 None:intergenic 35.0%
! TCGTTCATTATCTTGTTGTG+TGG + tig0023067:21628-21647 Msa1404460:three_prime_UTR 35.0%
TACAGACACAGCCATGATTT+CGG - tig0023067:19401-19420 None:intergenic 40.0%
!! AATCTCTTTTCAGGCCAAAG+TGG + tig0023067:19562-19581 Msa1404460:intron 40.0%
ACAGCAAAGACATCTAGTGA+CGG - tig0023067:19699-19718 None:intergenic 40.0%
! AGATGTCTTTGCTGTGGTAA+AGG + tig0023067:19704-19723 Msa1404460:CDS 40.0%
AACATCCAATGACATGTCGA+CGG - tig0023067:20094-20113 None:intergenic 40.0%
CATTTACGTAGTTGGGACAT+TGG - tig0023067:20230-20249 None:intergenic 40.0%
TGAATGGGAAAAGAGAGCTT+GGG + tig0023067:20537-20556 Msa1404460:intron 40.0%
!! AATAAATATGATGGGCCTCG+TGG - tig0023067:20563-20582 None:intergenic 40.0%
CAACTGTAAGATCCTACAGA+AGG - tig0023067:20738-20757 None:intergenic 40.0%
GGTTGAAAGATCATAAGTCG+AGG - tig0023067:20770-20789 None:intergenic 40.0%
TTCTATGTAAGATTGGGAGG+GGG - tig0023067:20791-20810 None:intergenic 40.0%
TGTTGCTGTAAAATCAGGAC+CGG + tig0023067:20863-20882 Msa1404460:intron 40.0%
CAACATAGAGAACAAGGTCA+AGG - tig0023067:21023-21042 None:intergenic 40.0%
CTTGACCTTGTTCTCTATGT+TGG + tig0023067:21021-21040 Msa1404460:CDS 40.0%
AGAAGCCAACATAGAGAACA+AGG - tig0023067:21029-21048 None:intergenic 40.0%
! AAGCACCAAAATGAAGGCAT+TGG + tig0023067:21097-21116 Msa1404460:CDS 40.0%
! CCAATCCAATGCCTTCATTT+TGG - tig0023067:21105-21124 None:intergenic 40.0%
!! CCAAAATGAAGGCATTGGAT+TGG + tig0023067:21102-21121 Msa1404460:CDS 40.0%
GTTGAAAGCAGTAAACCTTC+AGG - tig0023067:21161-21180 None:intergenic 40.0%
GTTTACTGCTTTCAACATGC+TGG + tig0023067:21165-21184 Msa1404460:three_prime_UTR 40.0%
!!! CCAAAATGAAGGTGTTTTGG+GGG + tig0023067:21222-21241 Msa1404460:three_prime_UTR 40.0%
!! AATAGATGGTAGTGGTATGG+TGG + tig0023067:21339-21358 Msa1404460:three_prime_UTR 40.0%
!! ATAGATGGTAGTGGTATGGT+GGG + tig0023067:21340-21359 Msa1404460:three_prime_UTR 40.0%
GATGCACTAAAGCAAATGGT+TGG - tig0023067:21425-21444 None:intergenic 40.0%
! TGACCATTCTTTCTGGAAAG+TGG - tig0023067:21525-21544 None:intergenic 40.0%
GGCGGTTCCAAAAAAAGATT+TGG - tig0023067:21607-21626 None:intergenic 40.0%
AACAAGATAATGAACGAGGG+CGG - tig0023067:21625-21644 None:intergenic 40.0%
GTACTCATCAGGAACCACTT+TGG - tig0023067:19579-19598 None:intergenic 45.0%
GAACAGTTTGACTGAGAAGC+AGG - tig0023067:19670-19689 None:intergenic 45.0%
GTCACTAGATGTCTTTGCTG+TGG + tig0023067:19698-19717 Msa1404460:CDS 45.0%
TGTGGTAAAGGTCTCATCTC+TGG + tig0023067:19716-19735 Msa1404460:CDS 45.0%
GAAGAAGATGACAGCACAAG+TGG - tig0023067:19750-19769 None:intergenic 45.0%
ACATCCAATGACATGTCGAC+GGG - tig0023067:20093-20112 None:intergenic 45.0%
TTACGTAGTTGGGACATTGG+TGG - tig0023067:20227-20246 None:intergenic 45.0%
TGTCCCAACTACGTAAATGC+AGG + tig0023067:20231-20250 Msa1404460:intron 45.0%
GTCCCAACTACGTAAATGCA+GGG + tig0023067:20232-20251 Msa1404460:intron 45.0%
TCCCAACTACGTAAATGCAG+GGG + tig0023067:20233-20252 Msa1404460:intron 45.0%
TCCCCTGCATTTACGTAGTT+GGG - tig0023067:20237-20256 None:intergenic 45.0%
ATCCCCTGCATTTACGTAGT+TGG - tig0023067:20238-20257 None:intergenic 45.0%
GATTACGTGCAACCGAAATC+GGG - tig0023067:20270-20289 None:intergenic 45.0%
GGATTACGTGCAACCGAAAT+CGG - tig0023067:20271-20290 None:intergenic 45.0%
CTTACGCTCTCTCACTTTCA+TGG - tig0023067:20320-20339 None:intergenic 45.0%
AGAAGGAGAGCAGATTGCAA+GGG - tig0023067:20404-20423 None:intergenic 45.0%
AAGAAGGAGAGCAGATTGCA+AGG - tig0023067:20405-20424 None:intergenic 45.0%
ATTATGAGAGTTGACCCCTC+AGG + tig0023067:20478-20497 Msa1404460:intron 45.0%
! ACTAGGGTTTTTCCGGTGAA+TGG + tig0023067:20521-20540 Msa1404460:intron 45.0%
! CTAGGGTTTTTCCGGTGAAT+GGG + tig0023067:20522-20541 Msa1404460:intron 45.0%
! AGCTCTCTTTTCCCATTCAC+CGG - tig0023067:20536-20555 None:intergenic 45.0%
GTGAATGGGAAAAGAGAGCT+TGG + tig0023067:20536-20555 Msa1404460:intron 45.0%
! GCACAATTTTGCCCTTCTGT+AGG + tig0023067:20723-20742 Msa1404460:intron 45.0%
AATTCCCTCGGTACTTTGTG+TGG - tig0023067:20940-20959 None:intergenic 45.0%
CGTGTGTCACAAAATTCCCT+CGG - tig0023067:20952-20971 None:intergenic 45.0%
!! TGGGAGAAGCACCAAAATGA+AGG + tig0023067:21091-21110 Msa1404460:CDS 45.0%
CTTCAGGGCTTATCAGATTC+AGG - tig0023067:21145-21164 None:intergenic 45.0%
CTGAATCTGATAAGCCCTGA+AGG + tig0023067:21143-21162 Msa1404460:CDS 45.0%
! CCCCCAAAACACCTTCATTT+TGG - tig0023067:21225-21244 None:intergenic 45.0%
!! TAGATGGTAGTGGTATGGTG+GGG + tig0023067:21341-21360 Msa1404460:three_prime_UTR 45.0%
!! TACAAAAAAAAAAAAAAAAA+GGG - tig0023067:19828-19847 None:intergenic 5.0%
!! TTACAAAAAAAAAAAAAAAA+AGG - tig0023067:19829-19848 None:intergenic 5.0%
GTTCGGTCATCGTACTCATC+AGG - tig0023067:19590-19609 None:intergenic 50.0%
CATCCAATGACATGTCGACG+GGG - tig0023067:20092-20111 None:intergenic 50.0%
! GATTGCCGATTGCCCGATTT+CGG + tig0023067:20255-20274 Msa1404460:intron 50.0%
TGCAACCGAAATCGGGCAAT+CGG - tig0023067:20263-20282 None:intergenic 50.0%
GAAGGAGAGCAGATTGCAAG+GGG - tig0023067:20403-20422 None:intergenic 50.0%
ATAGGGGAAGGCAGAGAAGA+AGG - tig0023067:20421-20440 None:intergenic 50.0%
!! CCTACCACACAAAGTACCGA+GGG + tig0023067:20933-20952 Msa1404460:CDS 50.0%
TCTTGCGCTACACTTCGCAA+AGG + tig0023067:20977-20996 Msa1404460:CDS 50.0%
! GCAAAGGTGTGTTTGCTGCT+GGG + tig0023067:20993-21012 Msa1404460:CDS 50.0%
! CAAAGGTGTGTTTGCTGCTG+GGG + tig0023067:20994-21013 Msa1404460:CDS 50.0%
!! CAAGGCTGACACTGGTTTCT+GGG + tig0023067:21072-21091 Msa1404460:CDS 50.0%
!! GGATTGGCCACACACAATCA+CGG + tig0023067:21118-21137 Msa1404460:CDS 50.0%
TTCAGGACCGTGATTGTGTG+TGG - tig0023067:21128-21147 None:intergenic 50.0%
!!! GAAGGTGTTTTGGGGGATGA+AGG + tig0023067:21229-21248 Msa1404460:three_prime_UTR 50.0%
!!! GTGTTTTGGGGGATGAAGGA+AGG + tig0023067:21233-21252 Msa1404460:three_prime_UTR 50.0%
!! AGATGGTAGTGGTATGGTGG+GGG + tig0023067:21342-21361 Msa1404460:three_prime_UTR 50.0%
ACCGACGCTTCCACTGTCTA+CGG + tig0023067:19626-19645 Msa1404460:CDS 55.0%
! ACCGTAGACAGTGGAAGCGT+CGG - tig0023067:19630-19649 None:intergenic 55.0%
AGCAGCAACACCGTAGACAG+TGG - tig0023067:19639-19658 None:intergenic 55.0%
TCGGATTGCTTTCCCGCAGT+TGG + tig0023067:20049-20068 Msa1404460:intron 55.0%
TGAGGGTCAACTGCCAACTG+CGG - tig0023067:20065-20084 None:intergenic 55.0%
CATGTCGACGGGGTGAATGA+GGG - tig0023067:20082-20101 None:intergenic 55.0%
ACATGTCGACGGGGTGAATG+AGG - tig0023067:20083-20102 None:intergenic 55.0%
TCACCCCGTCGACATGTCAT+TGG + tig0023067:20086-20105 Msa1404460:intron 55.0%
AAAAGAGAGCTTGGGCCACG+AGG + tig0023067:20545-20564 Msa1404460:intron 55.0%
!!! GACCGGTTTTTTGGCAGCAG+AGG + tig0023067:20880-20899 Msa1404460:CDS 55.0%
CGCCTCTGCTGCCAAAAAAC+CGG - tig0023067:20885-20904 None:intergenic 55.0%
! GCTTGTTAGCAGGCTCTGCT+AGG + tig0023067:20906-20925 Msa1404460:CDS 55.0%
!! GCCTACCACACAAAGTACCG+AGG + tig0023067:20932-20951 Msa1404460:CDS 55.0%
CCCTCGGTACTTTGTGTGGT+AGG - tig0023067:20936-20955 None:intergenic 55.0%
! CGCAAAGGTGTGTTTGCTGC+TGG + tig0023067:20992-21011 Msa1404460:CDS 55.0%
GTACTATTGGGCACACGCCA+AGG + tig0023067:21054-21073 Msa1404460:CDS 55.0%
CCAGAAACCAGTGTCAGCCT+TGG - tig0023067:21074-21093 None:intergenic 55.0%
!! CCAAGGCTGACACTGGTTTC+TGG + tig0023067:21071-21090 Msa1404460:CDS 55.0%
CGCTGAACGACGTCGTAGTG+AGG + tig0023067:19477-19496 Msa1404460:CDS 60.0%
!! GTAGACAGTGGAAGCGTCGG+TGG - tig0023067:19627-19646 None:intergenic 60.0%
GAGGGTCAACTGCCAACTGC+GGG - tig0023067:20064-20083 None:intergenic 60.0%
GGAGAGCAGATTGCAAGGGG+CGG - tig0023067:20400-20419 None:intergenic 60.0%
! GCAGAGGCGTGCTTGTTAGC+AGG + tig0023067:20896-20915 Msa1404460:CDS 60.0%
GCACACGCCAAGGCTGACAC+TGG + tig0023067:21064-21083 Msa1404460:CDS 65.0%
Chromosome Type Strat End Strand Name
tig0023067 gene 19253 21711 19253 ID=Msa1404460;Name=Msa1404460
tig0023067 mRNA 19253 21711 19253 ID=Msa1404460-mRNA-1;Parent=Msa1404460;Name=Msa1404460-mRNA-1;_AED=0.16;_eAED=0.16;_QI=153|1|1|1|1|1|3|549|193
tig0023067 exon 19253 19498 19253 ID=Msa1404460-mRNA-1:exon:23649;Parent=Msa1404460-mRNA-1
tig0023067 exon 19575 19780 19575 ID=Msa1404460-mRNA-1:exon:23650;Parent=Msa1404460-mRNA-1
tig0023067 exon 20880 21711 20880 ID=Msa1404460-mRNA-1:exon:23651;Parent=Msa1404460-mRNA-1
tig0023067 five_prime_UTR 19253 19405 19253 ID=Msa1404460-mRNA-1:five_prime_utr;Parent=Msa1404460-mRNA-1
tig0023067 CDS 19406 19498 19406 ID=Msa1404460-mRNA-1:cds;Parent=Msa1404460-mRNA-1
tig0023067 CDS 19575 19780 19575 ID=Msa1404460-mRNA-1:cds;Parent=Msa1404460-mRNA-1
tig0023067 CDS 20880 21162 20880 ID=Msa1404460-mRNA-1:cds;Parent=Msa1404460-mRNA-1
tig0023067 three_prime_UTR 21163 21711 21163 ID=Msa1404460-mRNA-1:three_prime_utr;Parent=Msa1404460-mRNA-1
Gene Sequence

>Msa1404460

ATGGCTGTGTCTGTAACAATTATAGTCCTTATCATTTCTCTTCATCTCTTAGCTTTCGTTTTTGCTATAGGCGCTGAACGACGTCGTAGTGAGGCCAAAGTGGTTCCTGATGAGTACGATGACCGAACTTTCTGTGTTTACACCACCGACGCTTCCACTGTCTACGGTGTTGCTGCTTTCTCTCTCCTGCTTCTCAGTCAAACTGTTCTTAACGCCGTCACTAGATGTCTTTGCTGTGGTAAAGGTCTCATCTCTGGTTGTTCTACCACTTGTGCTGTCATCTTCTTCATTCTCTCATGGACCGGTTTTTTGGCAGCAGAGGCGTGCTTGTTAGCAGGCTCTGCTAGGAATGCCTACCACACAAAGTACCGAGGGAATTTTGTGACACACGACTTGTCTTGCGCTACACTTCGCAAAGGTGTGTTTGCTGCTGGGGCTGCCTTGACCTTGTTCTCTATGTTGGCTTCTATTTTGTACTATTGGGCACACGCCAAGGCTGACACTGGTTTCTGGGAGAAGCACCAAAATGAAGGCATTGGATTGGCCACACACAATCACGGTCCTGAATCTGATAAGCCCTGA
Protein sequence

>Msa1404460

MAVSVTIIVLIISLHLLAFVFAIGAERRRSEAKVVPDEYDDRTFCVYTTDASTVYGVAAFSLLLLSQTVLNAVTRCLCCGKGLISGCSTTCAVIFFILSWTGFLAAEACLLAGSARNAYHTKYRGNFVTHDLSCATLRKGVFAAGAALTLFSMLASILYYWAHAKADTGFWEKHQNEGIGLATHNHGPESDKP*