Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1410330 | A0A072UJ08 | 64.474 | 76 | 14 | 3 | 8 | 72 | 35 | 108 | 5.45e-13 | 70.1 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0010710 | Msa1410330 | 0.852002 | 6.140997e-61 | -8.615850e-47 |
| Msa1270230 | Msa1410330 | 0.810038 | 1.405768e-50 | -8.615850e-47 |
| Msa1285930 | Msa1410330 | 0.801050 | 1.081078e-48 | -8.615850e-47 |
| Msa1287810 | Msa1410330 | 0.821824 | 3.288518e-53 | -8.615850e-47 |
| Msa0414420 | Msa1410330 | 0.816455 | 5.482638e-52 | -8.615850e-47 |
| Msa0452900 | Msa1410330 | 0.810586 | 1.070786e-50 | -8.615850e-47 |
| Msa0342240 | Msa1410330 | 0.800743 | 1.248755e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGTGGGTTGTGTTGTGTT+GGG | 0.203986 | tig0023648:+82150 | Msa1410330:CDS |
| CATTGTCTCTTACTTGTGTT+CGG | 0.305532 | tig0023648:+82091 | Msa1410330:CDS |
| GCTTGTGGGTTGTGTTGTGT+TGG | 0.310318 | tig0023648:+82149 | Msa1410330:CDS |
| ATTGTCTCTTACTTGTGTTC+GGG | 0.356017 | tig0023648:+82092 | Msa1410330:CDS |
| TTGTGGGTTGTGTTGTGTTG+GGG | 0.401258 | tig0023648:+82151 | Msa1410330:CDS |
| AGGGAGTGGTGTTGCCGTCT+TGG | 0.433095 | tig0023648:+81949 | Msa1410330:CDS |
| GGGAGTGGTGTTGCCGTCTT+GGG | 0.451301 | tig0023648:+81950 | Msa1410330:CDS |
| GTTTCGAATGGACGTGGTGA+AGG | 0.492737 | tig0023648:+81929 | Msa1410330:CDS |
| TGTGGGTTGTGTTGTGTTGG+GGG | 0.521046 | tig0023648:+82152 | Msa1410330:CDS |
| CAGACTGCATCACCACCACT+TGG | 0.546348 | tig0023648:-81979 | None:intergenic |
| TGATGATGATGAGGAGGAGG+AGG | 0.560821 | tig0023648:+82125 | Msa1410330:CDS |
| CGATGATGATGATGAGGAGG+AGG | 0.566861 | tig0023648:+82122 | Msa1410330:CDS |
| TTGTCTCTTACTTGTGTTCG+GGG | 0.578818 | tig0023648:+82093 | Msa1410330:CDS |
| TTTCGAATGGACGTGGTGAA+GGG | 0.582022 | tig0023648:+81930 | Msa1410330:CDS |
| GTGTGGCGATGATGATGATG+AGG | 0.596717 | tig0023648:+82116 | Msa1410330:CDS |
| AATGGACGTGGTGAAGGGAG+TGG | 0.607337 | tig0023648:+81935 | Msa1410330:CDS |
| GGTGAAGTTTCGAATGGACG+TGG | 0.620357 | tig0023648:+81923 | Msa1410330:CDS |
| CACTTGGACATCTCCCAAGA+CGG | 0.633352 | tig0023648:-81963 | None:intergenic |
| TGGCGATGATGATGATGAGG+AGG | 0.645968 | tig0023648:+82119 | Msa1410330:CDS |
| GTGTGGTGATCTCAGATCGA+AGG | 0.648116 | tig0023648:+82003 | Msa1410330:CDS |
| TGTCTCTTACTTGTGTTCGG+GGG | 0.659716 | tig0023648:+82094 | Msa1410330:CDS |
| CTTGGGAGATGTCCAAGTGG+TGG | 0.686922 | tig0023648:+81967 | Msa1410330:CDS |
| GTGGTGATGCAGTCTGCGTG+TGG | 0.689294 | tig0023648:+81986 | Msa1410330:CDS |
| CGTCTTGGGAGATGTCCAAG+TGG | 0.753276 | tig0023648:+81964 | Msa1410330:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTCATGATTTATTGAGTTGA+TGG | + | tig0023648:82025-82044 | Msa1410330:intron | 30.0% |
| !!! | TATTGAGTTGATGGTGATTT+TGG | + | tig0023648:82034-82053 | Msa1410330:intron | 30.0% |
| !!! | GTTTTTGGTGAAGTTTCGAA+TGG | + | tig0023648:81917-81936 | Msa1410330:CDS | 35.0% |
| CATTGTCTCTTACTTGTGTT+CGG | + | tig0023648:82091-82110 | Msa1410330:CDS | 35.0% | |
| ATTGTCTCTTACTTGTGTTC+GGG | + | tig0023648:82092-82111 | Msa1410330:CDS | 35.0% | |
| !!! | TGAGTTGATGGTGATTTTGG+TGG | + | tig0023648:82037-82056 | Msa1410330:intron | 40.0% |
| !! | ATGGTGATTTTGGTGGTTGT+TGG | + | tig0023648:82044-82063 | Msa1410330:intron | 40.0% |
| TTGTCTCTTACTTGTGTTCG+GGG | + | tig0023648:82093-82112 | Msa1410330:CDS | 40.0% | |
| TTTCGAATGGACGTGGTGAA+GGG | + | tig0023648:81930-81949 | Msa1410330:CDS | 45.0% | |
| !! | GTGGTTGTTGGTGTTGAGTT+GGG | + | tig0023648:82056-82075 | Msa1410330:intron | 45.0% |
| TGTCTCTTACTTGTGTTCGG+GGG | + | tig0023648:82094-82113 | Msa1410330:CDS | 45.0% | |
| ! | CTTGTGGGTTGTGTTGTGTT+GGG | + | tig0023648:82150-82169 | Msa1410330:CDS | 45.0% |
| ! | TTGTGGGTTGTGTTGTGTTG+GGG | + | tig0023648:82151-82170 | Msa1410330:CDS | 45.0% |
| GGTGAAGTTTCGAATGGACG+TGG | + | tig0023648:81923-81942 | Msa1410330:CDS | 50.0% | |
| GTTTCGAATGGACGTGGTGA+AGG | + | tig0023648:81929-81948 | Msa1410330:CDS | 50.0% | |
| CACTTGGACATCTCCCAAGA+CGG | - | tig0023648:81966-81985 | None:intergenic | 50.0% | |
| GTGTGGTGATCTCAGATCGA+AGG | + | tig0023648:82003-82022 | Msa1410330:CDS | 50.0% | |
| !! | GGTGGTTGTTGGTGTTGAGT+TGG | + | tig0023648:82055-82074 | Msa1410330:intron | 50.0% |
| !! | GTTGTTGGTGTTGAGTTGGG+AGG | + | tig0023648:82059-82078 | Msa1410330:intron | 50.0% |
| ! | GTGTGGCGATGATGATGATG+AGG | + | tig0023648:82116-82135 | Msa1410330:CDS | 50.0% |
| ! | TGGCGATGATGATGATGAGG+AGG | + | tig0023648:82119-82138 | Msa1410330:CDS | 50.0% |
| ! | CGATGATGATGATGAGGAGG+AGG | + | tig0023648:82122-82141 | Msa1410330:CDS | 50.0% |
| ! | TGATGATGATGAGGAGGAGG+AGG | + | tig0023648:82125-82144 | Msa1410330:CDS | 50.0% |
| ! | GCTTGTGGGTTGTGTTGTGT+TGG | + | tig0023648:82149-82168 | Msa1410330:CDS | 50.0% |
| ! | TGTGGGTTGTGTTGTGTTGG+GGG | + | tig0023648:82152-82171 | Msa1410330:CDS | 50.0% |
| AATGGACGTGGTGAAGGGAG+TGG | + | tig0023648:81935-81954 | Msa1410330:CDS | 55.0% | |
| CGTCTTGGGAGATGTCCAAG+TGG | + | tig0023648:81964-81983 | Msa1410330:CDS | 55.0% | |
| CTTGGGAGATGTCCAAGTGG+TGG | + | tig0023648:81967-81986 | Msa1410330:CDS | 55.0% | |
| CAGACTGCATCACCACCACT+TGG | - | tig0023648:81982-82001 | None:intergenic | 55.0% | |
| !! | GTTGGTGTTGAGTTGGGAGG+TGG | + | tig0023648:82062-82081 | Msa1410330:intron | 55.0% |
| CTTACTTGTGTTCGGGGGTG+TGG | + | tig0023648:82099-82118 | Msa1410330:CDS | 55.0% | |
| !! | AGGGAGTGGTGTTGCCGTCT+TGG | + | tig0023648:81949-81968 | Msa1410330:CDS | 60.0% |
| !! | GGGAGTGGTGTTGCCGTCTT+GGG | + | tig0023648:81950-81969 | Msa1410330:CDS | 60.0% |
| ! | GTGGTGATGCAGTCTGCGTG+TGG | + | tig0023648:81986-82005 | Msa1410330:CDS | 60.0% |
| TGAGGAGGAGGAGGAGCTTG+TGG | + | tig0023648:82134-82153 | Msa1410330:CDS | 60.0% | |
| GAGGAGGAGGAGGAGCTTGT+GGG | + | tig0023648:82135-82154 | Msa1410330:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0023648 | gene | 81908 | 82182 | 81908 | ID=Msa1410330;Name=Msa1410330 |
| tig0023648 | mRNA | 81908 | 82182 | 81908 | ID=Msa1410330-mRNA-1;Parent=Msa1410330;Name=Msa1410330-mRNA-1;_AED=0.41;_eAED=0.57;_QI=0|0|0|1|0|0|2|0|72 |
| tig0023648 | exon | 81908 | 82024 | 81908 | ID=Msa1410330-mRNA-1:exon:26512;Parent=Msa1410330-mRNA-1 |
| tig0023648 | exon | 82081 | 82182 | 82081 | ID=Msa1410330-mRNA-1:exon:26513;Parent=Msa1410330-mRNA-1 |
| tig0023648 | CDS | 81908 | 82024 | 81908 | ID=Msa1410330-mRNA-1:cds;Parent=Msa1410330-mRNA-1 |
| tig0023648 | CDS | 82081 | 82182 | 82081 | ID=Msa1410330-mRNA-1:cds;Parent=Msa1410330-mRNA-1 |
| Gene Sequence |
| Protein sequence |
