AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0414420


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0414420 MtrunA17_Chr3g0107461 88.820 161 18 0 1 161 1 161 9.26e-106 299
Msa0414420 MtrunA17_Chr4g0031421 79.699 133 27 0 29 161 1 133 2.69e-74 219
Msa0414420 MtrunA17_Chr3g0105791 78.947 133 28 0 29 161 1 133 7.69e-73 215
Msa0414420 MtrunA17_Chr3g0107381 53.247 154 71 1 1 154 1 153 8.25e-55 172
Msa0414420 MtrunA17_Chr3g0110131 48.101 158 81 1 1 158 1 157 1.07e-41 139
Msa0414420 MtrunA17_Chr2g0283941 41.875 160 93 0 1 160 1 160 7.42e-41 137
Msa0414420 MtrunA17_Chr2g0296861 47.742 155 80 1 1 155 1 154 1.86e-40 133
Msa0414420 MtrunA17_Chr5g0432691 40.909 154 90 1 1 154 1 153 4.71e-38 127
Msa0414420 MtrunA17_Chr8g0351751 41.139 158 91 1 1 156 1 158 1.52e-36 126
Msa0414420 MtrunA17_Chr4g0014321 38.312 154 95 0 1 154 1 154 8.23e-36 124
Msa0414420 MtrunA17_Chr3g0107331 40.397 151 89 1 3 153 2 151 9.37e-34 116
Msa0414420 MtrunA17_Chr3g0106421 46.512 129 69 0 29 157 1 129 9.83e-34 118
Msa0414420 MtrunA17_Chr4g0014131 42.236 161 93 0 1 161 1 161 2.27e-33 117
Msa0414420 MtrunA17_Chr4g0014031 41.615 161 94 0 1 161 1 161 9.55e-33 116
Msa0414420 MtrunA17_Chr2g0296831 47.541 122 64 0 1 122 1 122 6.10e-32 110
Msa0414420 MtrunA17_Chr3g0106501 43.939 132 74 0 29 160 1 132 2.20e-31 110
Msa0414420 MtrunA17_Chr4g0014121 38.365 159 98 0 1 159 29 187 2.48e-30 108
Msa0414420 MtrunA17_Chr3g0107351 36.129 155 98 1 3 157 2 155 8.22e-30 106
Msa0414420 MtrunA17_Chr5g0420351 37.580 157 96 2 1 157 1 155 9.11e-30 106
Msa0414420 MtrunA17_Chr4g0013041 36.364 154 96 1 1 154 1 152 1.09e-29 107
Msa0414420 MtrunA17_Chr3g0107341 36.129 155 98 1 3 157 2 155 7.88e-28 101
Msa0414420 MtrunA17_Chr3g0107361 36.129 155 98 1 3 157 2 155 9.88e-28 101
Msa0414420 MtrunA17_Chr1g0188681 38.272 162 92 4 1 159 1 157 1.59e-27 100
Msa0414420 MtrunA17_Chr1g0197461 35.484 155 98 2 3 157 2 154 3.20e-27 99.8
Msa0414420 MtrunA17_Chr1g0188661 35.802 162 100 3 1 161 1 159 2.73e-25 95.1
Msa0414420 MtrunA17_Chr4g0012761 36.025 161 101 2 1 161 1 159 4.49e-25 94.4
Msa0414420 MtrunA17_Chr1g0197261 32.911 158 102 3 1 157 1 155 5.94e-24 91.3
Msa0414420 MtrunA17_Chr1g0188651 36.025 161 99 2 1 161 1 157 7.63e-24 91.3
Msa0414420 MtrunA17_Chr1g0197321 33.962 159 100 3 1 157 1 156 1.95e-23 90.1
Msa0414420 MtrunA17_Chr7g0218151 34.161 161 104 2 1 161 1 159 2.11e-23 90.1
Msa0414420 MtrunA17_Chr1g0193311 36.076 158 97 3 1 157 1 155 1.12e-22 88.2
Msa0414420 MtrunA17_Chr4g0008701 28.148 135 93 1 16 150 19 149 2.67e-14 69.3
Msa0414420 MtrunA17_Chr7g0267601 28.070 114 82 0 5 118 8 121 1.10e-12 64.7
Msa0414420 MtrunA17_Chr3g0128721 26.056 142 96 2 16 153 19 155 4.00e-12 63.2
Msa0414420 MtrunA17_Chr6g0457851 29.204 113 73 3 1 107 11 122 1.68e-11 61.2
Msa0414420 MtrunA17_Chr1g0212151 28.448 116 76 3 1 110 1 115 4.17e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0414420 AT5G48670.1 48.077 156 78 2 1 155 1 154 2.57e-37 130
Msa0414420 AT3G05860.2 51.515 99 48 0 1 99 1 99 1.27e-29 107
Msa0414420 AT1G65330.1 41.216 148 86 1 3 150 2 148 1.28e-29 109
Msa0414420 AT3G05860.3 51.515 99 48 0 1 99 1 99 2.36e-29 108
Msa0414420 AT1G65300.1 39.735 151 90 1 5 155 4 153 4.33e-29 108
Msa0414420 AT3G05860.1 51.515 99 48 0 1 99 1 99 4.44e-29 107
Msa0414420 AT5G27810.1 51.685 89 43 0 29 117 1 89 3.99e-28 101
Msa0414420 AT5G26650.1 37.255 153 95 1 5 157 3 154 7.33e-26 101
Msa0414420 AT5G27960.1 35.948 153 97 1 5 157 3 154 1.13e-24 97.4
Msa0414420 AT1G22590.2 34.375 160 88 2 1 157 1 146 7.16e-24 91.7
Msa0414420 AT1G31630.1 36.875 160 83 5 3 154 2 151 3.74e-23 93.6
Msa0414420 AT1G31640.1 37.654 162 87 4 3 158 2 155 1.47e-22 93.2
Msa0414420 AT2G28700.1 33.333 150 99 1 1 150 1 149 2.70e-21 88.6
Msa0414420 AT5G26630.1 33.548 155 102 1 1 155 1 154 4.70e-20 83.2
Msa0414420 AT5G06500.1 32.653 147 89 3 1 147 1 137 3.92e-18 78.6
Msa0414420 AT2G40210.1 35.644 101 65 0 1 101 1 101 4.04e-14 68.9
Msa0414420 AT5G26580.1 38.614 101 48 1 1 101 1 87 8.39e-14 68.2
Msa0414420 AT3G57230.1 28.395 162 101 4 1 161 1 148 1.84e-11 60.5
Msa0414420 AT3G57230.4 28.395 162 101 4 1 161 1 148 1.84e-11 60.5
Msa0414420 AT3G57230.2 29.825 114 76 2 1 113 1 111 4.82e-11 57.8

Find 31 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGTCATTTCCTGCTTTC+TGG 0.066665 3_2:+59931461 None:intergenic
ATTCATACTACAGAATTTAA+TGG 0.196701 3_2:-59931423 Msa0414420:CDS
CAAGTTTGGCCATCAGATTC+AGG 0.225208 3_2:-59931618 Msa0414420:CDS
CTGAATGTTATCCTCCTCTT+CGG 0.350265 3_2:+59931938 None:intergenic
GTTCAGAAGTTTGCCTGGAC+TGG 0.390262 3_2:-59931574 Msa0414420:CDS
ACAATCCTCAAGCACAAGTT+TGG 0.390704 3_2:-59931632 Msa0414420:CDS
TGAATGTTATCCTCCTCTTC+GGG 0.395416 3_2:+59931939 None:intergenic
GATGAAATTAGCACTCTTTG+CGG 0.422591 3_2:-59931687 Msa0414420:CDS
AATGAAATGATGAATGATAT+TGG 0.442815 3_2:+59931443 None:intergenic
TATTCTTGAGGCAAAGCATT+GGG 0.452810 3_2:-59931521 Msa0414420:CDS
TCCAGGTTCAGAAGTTTGCC+TGG 0.467504 3_2:-59931579 Msa0414420:CDS
AAAGAAGGAAACCAGAAAGC+AGG 0.481583 3_2:-59931472 Msa0414420:CDS
AAGTTTGGCCATCAGATTCA+GGG 0.497829 3_2:-59931617 Msa0414420:CDS
GGGTTAGAAGTGTGATATCC+AGG 0.506627 3_2:-59931596 Msa0414420:CDS
CACTATCATGGCTAGAGGTA+AGG 0.508320 3_2:-59931987 None:intergenic
CAAATTCTCAGCAATAAAAG+AGG 0.520681 3_2:+59931365 None:intergenic
GGAGGATAACATTCAGAAAG+AGG 0.525464 3_2:-59931931 Msa0414420:CDS
TCCAGGCAAACTTCTGAACC+TGG 0.543284 3_2:+59931578 None:intergenic
GTATTCTTGAGGCAAAGCAT+TGG 0.555152 3_2:-59931522 Msa0414420:CDS
GCAGTTGATGAAACAAAAGA+AGG 0.561272 3_2:-59931487 Msa0414420:CDS
TTGCTCGTAAATTATTGCAC+CGG 0.562136 3_2:+59931656 None:intergenic
GATGGCCAAACTTGTGCTTG+AGG 0.578295 3_2:+59931627 None:intergenic
ACTCTTTGCGGAGTAGAAGC+CGG 0.579730 3_2:-59931675 Msa0414420:CDS
GAGGTCGATCAAAAGATGAA+AGG 0.605953 3_2:-59931339 Msa0414420:CDS
CTTCTAACCCCTGAATCTGA+TGG 0.608216 3_2:+59931609 None:intergenic
ACATAACATGTGACCCGAAG+AGG 0.618667 3_2:-59931952 Msa0414420:CDS
AGTTTGGCCATCAGATTCAG+GGG 0.628974 3_2:-59931616 Msa0414420:CDS
TATTGCTGAGAATTTGAGGG+AGG 0.639350 3_2:-59931358 Msa0414420:CDS
ATCCTCACTATCATGGCTAG+AGG 0.659018 3_2:-59931992 None:intergenic
TAACATGTGACCCGAAGAGG+AGG 0.659370 3_2:-59931949 Msa0414420:CDS
TGGATCAAGAGGTATTCTTG+AGG 0.709854 3_2:-59931533 Msa0414420:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATGGATTTTTTTTAATA+GGG - chr3_2:59931575-59931594 Msa0414420:CDS 10.0%
!!! TAAATTTGGAATTTTACTAT+AGG - chr3_2:59931542-59931561 Msa0414420:CDS 15.0%
!! TATAGGTTATATATTGTTTA+TGG - chr3_2:59931559-59931578 Msa0414420:CDS 15.0%
!!! GTTTATGGATTTTTTTTAAT+AGG - chr3_2:59931574-59931593 Msa0414420:CDS 15.0%
!! AATGAAATGATGAATGATAT+TGG + chr3_2:59931860-59931879 None:intergenic 20.0%
!! ATTCATACTACAGAATTTAA+TGG - chr3_2:59931877-59931896 Msa0414420:intron 20.0%
!! TTTTATTGCTGAGAATTTGA+GGG - chr3_2:59931939-59931958 Msa0414420:CDS 25.0%
ACATTCAGAAAGAGGAAAAA+TGG - chr3_2:59931377-59931396 Msa0414420:CDS 30.0%
TATGTATGATATGCAAAGAG+AGG + chr3_2:59931413-59931432 None:intergenic 30.0%
ACTGGAACAAAGTAAAAAGA+TGG - chr3_2:59931744-59931763 Msa0414420:intron 30.0%
CAAATTCTCAGCAATAAAAG+AGG + chr3_2:59931938-59931957 None:intergenic 30.0%
! CTTTTATTGCTGAGAATTTG+AGG - chr3_2:59931938-59931957 Msa0414420:CDS 30.0%
GATGAAATTAGCACTCTTTG+CGG - chr3_2:59931613-59931632 Msa0414420:CDS 35.0%
TTGCTCGTAAATTATTGCAC+CGG + chr3_2:59931647-59931666 None:intergenic 35.0%
! TTTTTACTTTGTTCCAGTCC+AGG + chr3_2:59931742-59931761 None:intergenic 35.0%
GGAACAAAGTAAAAAGATGG+TGG - chr3_2:59931747-59931766 Msa0414420:intron 35.0%
!! TAAAAAGATGGTGGATCAAG+AGG - chr3_2:59931756-59931775 Msa0414420:intron 35.0%
! TATTCTTGAGGCAAAGCATT+GGG - chr3_2:59931779-59931798 Msa0414420:intron 35.0%
!! GCAGTTGATGAAACAAAAGA+AGG - chr3_2:59931813-59931832 Msa0414420:intron 35.0%
TGAATGTTATCCTCCTCTTC+GGG + chr3_2:59931364-59931383 None:intergenic 40.0%
CTGAATGTTATCCTCCTCTT+CGG + chr3_2:59931365-59931384 None:intergenic 40.0%
GGAGGATAACATTCAGAAAG+AGG - chr3_2:59931369-59931388 Msa0414420:CDS 40.0%
ACAATCCTCAAGCACAAGTT+TGG - chr3_2:59931668-59931687 Msa0414420:CDS 40.0%
AAGTTTGGCCATCAGATTCA+GGG - chr3_2:59931683-59931702 Msa0414420:CDS 40.0%
!! TGGATCAAGAGGTATTCTTG+AGG - chr3_2:59931767-59931786 Msa0414420:intron 40.0%
GTATTCTTGAGGCAAAGCAT+TGG - chr3_2:59931778-59931797 Msa0414420:intron 40.0%
AAAGAAGGAAACCAGAAAGC+AGG - chr3_2:59931828-59931847 Msa0414420:intron 40.0%
ATTGGTCATTTCCTGCTTTC+TGG + chr3_2:59931842-59931861 None:intergenic 40.0%
TATTGCTGAGAATTTGAGGG+AGG - chr3_2:59931942-59931961 Msa0414420:CDS 40.0%
GAGGTCGATCAAAAGATGAA+AGG - chr3_2:59931961-59931980 Msa0414420:CDS 40.0%
ACATAACATGTGACCCGAAG+AGG - chr3_2:59931348-59931367 Msa0414420:CDS 45.0%
CAAGTTTGGCCATCAGATTC+AGG - chr3_2:59931682-59931701 Msa0414420:CDS 45.0%
AGTTTGGCCATCAGATTCAG+GGG - chr3_2:59931684-59931703 Msa0414420:CDS 45.0%
CTTCTAACCCCTGAATCTGA+TGG + chr3_2:59931694-59931713 None:intergenic 45.0%
! GGGTTAGAAGTGTGATATCC+AGG - chr3_2:59931704-59931723 Msa0414420:CDS 45.0%
!!! TAACTTTTATTTAATAAATT+TGG - chr3_2:59931528-59931547 Msa0414420:CDS 5.0%
TAACATGTGACCCGAAGAGG+AGG - chr3_2:59931351-59931370 Msa0414420:CDS 50.0%
ACTCTTTGCGGAGTAGAAGC+CGG - chr3_2:59931625-59931644 Msa0414420:CDS 50.0%
GATGGCCAAACTTGTGCTTG+AGG + chr3_2:59931676-59931695 None:intergenic 50.0%
TCCAGGTTCAGAAGTTTGCC+TGG - chr3_2:59931721-59931740 Msa0414420:intron 50.0%
TCCAGGCAAACTTCTGAACC+TGG + chr3_2:59931725-59931744 None:intergenic 50.0%
GTTCAGAAGTTTGCCTGGAC+TGG - chr3_2:59931726-59931745 Msa0414420:intron 50.0%
Chromosome Type Strat End Strand Name
chr3_2 gene 59931320 59932002 59931320 ID=Msa0414420;Name=Msa0414420
chr3_2 mRNA 59931320 59932002 59931320 ID=Msa0414420-mRNA-1;Parent=Msa0414420;Name=Msa0414420-mRNA-1;_AED=0.08;_eAED=0.08;_QI=0|0|0|1|1|1|2|0|161
chr3_2 exon 59931924 59932002 59931924 ID=Msa0414420-mRNA-1:exon:12475;Parent=Msa0414420-mRNA-1
chr3_2 exon 59931320 59931726 59931320 ID=Msa0414420-mRNA-1:exon:12474;Parent=Msa0414420-mRNA-1
chr3_2 CDS 59931924 59932002 59931924 ID=Msa0414420-mRNA-1:cds;Parent=Msa0414420-mRNA-1
chr3_2 CDS 59931320 59931726 59931320 ID=Msa0414420-mRNA-1:cds;Parent=Msa0414420-mRNA-1
Gene Sequence

>Msa0414420

ATGGCTAGAGGTAAGGTGAAACTAGCATACATAACATGTGACCCGAAGAGGAGGATAACATTCAGAAAGAGGAAAAATGGGATTATGAAGAAGATTGATGAAATTAGCACTCTTTGCGGAGTAGAAGCCGGTGCAATAATTTACGAGCAAAACAATCCTCAAGCACAAGTTTGGCCATCAGATTCAGGGGTTAGAAGTGTGATATCCAGGTTCAGAAGTTTGCCTGGACTGGAACAAAGTAAAAAGATGGTGGATCAAGAGGTATTCTTGAGGCAAAGCATTGGGAAAGTCTATGAGCAGTTGATGAAACAAAAGAAGGAAACCAGAAAGCAGGAAATGACCAATATCATTCATCATTTCATTCATACTACAGAATTTAATGGATATTTAATGAGCAAACATGATCTTAATGATTTGTCCTCTTTTATTGCTGAGAATTTGAGGGAGGTCGATCAAAAGATGAAAGGAATGTCAATCGAAGATTGA

Protein sequence

>Msa0414420

MARGKVKLAYITCDPKRRITFRKRKNGIMKKIDEISTLCGVEAGAIIYEQNNPQAQVWPSDSGVRSVISRFRSLPGLEQSKKMVDQEVFLRQSIGKVYEQLMKQKKETRKQEMTNIIHHFIHTTEFNGYLMSKHDLNDLSSFIAENLREVDQKMKGMSIED*