AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1417980

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1417980 MtrunA17_Chr4g0076251 61.511 278 20 5 1 191 293 570 5.91e-97 293
Msa1417980 MtrunA17_Chr2g0290411 39.789 284 64 9 1 191 280 549 3.23e-39 141
Msa1417980 MtrunA17_Chr8g0344311 34.400 250 84 11 1 172 320 567 6.75e-15 72.8
Msa1417980 MtrunA17_Chr8g0391771 32.411 253 87 10 1 172 324 573 3.28e-13 67.8
Msa1417980 MtrunA17_Chr1g0161371 30.279 251 95 8 1 172 319 568 3.55e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1417980 AT4G17220.1 37.168 226 70 7 1 168 273 484 4.88e-27 107
Msa1417980 AT1G24764.2 31.907 257 85 10 1 172 188 439 9.57e-14 69.7
Msa1417980 AT1G24764.3 31.907 257 85 10 1 172 400 651 1.29e-13 69.3
Msa1417980 AT1G24764.1 31.907 257 85 10 1 172 341 592 1.34e-13 69.3
Msa1417980 AT2G01750.2 29.496 278 94 10 1 188 340 605 9.03e-13 67.0
Msa1417980 AT2G01750.1 29.496 278 94 10 1 188 340 605 9.28e-13 66.6
Msa1417980 AT1G68060.1 28.571 266 108 8 1 187 333 595 3.29e-12 65.1

Find 44 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACTATCAGCAACTTTACT+TGG 0.264053 tig0024533:+16675 None:intergenic
ATAAGTTCCCCAACAGAAAA+TGG 0.325320 tig0024533:-16867 Msa1417980:CDS
TTCACCATTTGCAATTCTAT+TGG 0.325874 tig0024533:+17689 None:intergenic
TATCAGCCACACTAACCTTT+TGG 0.326069 tig0024533:+15870 None:intergenic
AGTCATTTGGTTTCTTGCTT+TGG 0.339262 tig0024533:+16550 None:intergenic
TGCAGTGACGATTTGGTGTC+AGG 0.351949 tig0024533:-16525 Msa1417980:CDS
TGATATATTGACAGTTTGTT+TGG 0.367532 tig0024533:-17805 Msa1417980:five_prime_UTR
TCTTCTAGCCATTGCTTGAC+AGG 0.383618 tig0024533:+17630 None:intergenic
CCGCTCTACTTCCATGGACT+TGG 0.386322 tig0024533:+15987 None:intergenic
CTTCACTTTCAGCATTGAAC+TGG 0.389403 tig0024533:+16607 None:intergenic
TAATAAGTTCAGATGATAAC+AGG 0.405133 tig0024533:-15915 Msa1417980:CDS
CAGAAAACATGTTGAAGAAA+AGG 0.410590 tig0024533:-16700 Msa1417980:CDS
GCTAGCAAGGGTAAAAGTTT+CGG 0.430627 tig0024533:-17712 Msa1417980:five_prime_UTR
TCGTCACTGCACGAGTCATT+TGG 0.432134 tig0024533:+16537 None:intergenic
TGAAAAGGAAAATGAGATAC+AGG 0.451771 tig0024533:-16652 Msa1417980:CDS
AGTTGCTGATAGTGGTGAAA+AGG 0.454137 tig0024533:-16667 Msa1417980:CDS
TTTCTTCCGCTCTACTTCCA+TGG 0.458047 tig0024533:+15981 None:intergenic
TGACTCGTGCAGTGACGATT+TGG 0.459803 tig0024533:-16532 Msa1417980:CDS
CTTCCAATCGCAGAAGCTCT+TGG 0.464587 tig0024533:+16816 None:intergenic
CAACCAAGAGCTTCTGCGAT+TGG 0.480093 tig0024533:-16819 Msa1417980:CDS
CACTAGAGAGGGAGCTAGCA+AGG 0.481581 tig0024533:-17725 Msa1417980:five_prime_UTR
CCAAGTAAAGTTGCTGATAG+TGG 0.491571 tig0024533:-16675 Msa1417980:CDS
TTTGTTTGGTTGATCTTATG+TGG 0.496728 tig0024533:-17791 Msa1417980:five_prime_UTR
AGATGCAGCTGCTAGAGAAA+AGG 0.532223 tig0024533:-15950 Msa1417980:CDS
GAAACAAAGACTCCTCCAAA+AGG 0.543913 tig0024533:-15885 Msa1417980:CDS
TCGGCCAATAGAATTGCAAA+TGG 0.544219 tig0024533:-17693 Msa1417980:five_prime_UTR
TGATCTTATGTGGTAACTTG+TGG 0.547591 tig0024533:-17781 Msa1417980:five_prime_UTR
TGATGCACTGACCAAGTCCA+TGG 0.550345 tig0024533:-15998 Msa1417980:CDS
AGAAGAGAGAAGGATTATGC+AGG 0.567503 tig0024533:-17613 Msa1417980:intron
TGCAGCTGCTAGAGAAAAGG+AGG 0.568762 tig0024533:-15947 Msa1417980:CDS
AGCAATGGCTAGAAGAGAGA+AGG 0.577331 tig0024533:-17623 Msa1417980:CDS
AGGTCATGCCTGTCAAGCAA+TGG 0.604618 tig0024533:-17638 Msa1417980:exon
ATGAGAAAAGGACACTAGAG+AGG 0.607096 tig0024533:-17737 Msa1417980:five_prime_UTR
GTATGATAGGCTGCAAAGAG+AGG 0.608475 tig0024533:-16496 Msa1417980:intron
ACTCCTCCAAAAGGTTAGTG+TGG 0.610939 tig0024533:-15876 Msa1417980:CDS
ACTAGAGAGGGAGCTAGCAA+GGG 0.615439 tig0024533:-17724 Msa1417980:five_prime_UTR
TGGTGAAGCTAATGAGTGGA+AGG 0.633476 tig0024533:-17673 Msa1417980:five_prime_UTR
TGAGAAAAGGACACTAGAGA+GGG 0.660251 tig0024533:-17736 Msa1417980:five_prime_UTR
AAAGGAAAATGAGATACAGG+TGG 0.667770 tig0024533:-16649 Msa1417980:CDS
AGAAGCTCTTGGTTGTGATG+TGG 0.668929 tig0024533:+16827 None:intergenic
CCAAGTCCATGGAAGTAGAG+CGG 0.682402 tig0024533:-15987 Msa1417980:CDS
GTGGAAGGATGAAAATGACA+AGG 0.684047 tig0024533:-17658 Msa1417980:five_prime_UTR
CAAATGGTGAAGCTAATGAG+TGG 0.695992 tig0024533:-17677 Msa1417980:five_prime_UTR
AGTTCCCCAACAGAAAATGG+TGG 0.700541 tig0024533:-16864 Msa1417980:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAATTGTAGTATTTA+AGG + tig0024533:16154-16173 None:intergenic 10.0%
!! AAATAAAATTGTAGTATTTA+AGG + tig0024533:16154-16173 None:intergenic 10.0%
!!! ATTTTATTTTGTTACTTATG+AGG - tig0024533:16165-16184 Msa1417980:intron 15.0%
!! ATATACTGAAATAAATCTTA+AGG - tig0024533:16214-16233 Msa1417980:intron 15.0%
!! TATACTGAAATAAATCTTAA+GGG - tig0024533:16215-16234 Msa1417980:intron 15.0%
!! GAAGTATATTTCATATTAAT+AGG + tig0024533:16956-16975 None:intergenic 15.0%
!!! TAATTTTCGTAAATTATTAC+CGG - tig0024533:17415-17434 Msa1417980:intron 15.0%
!!! CATCTATTTCTTTTTTAAAA+TGG + tig0024533:17523-17542 None:intergenic 15.0%
!!! ATTTTATTTTGTTACTTATG+AGG - tig0024533:16165-16184 Msa1417980:intron 15.0%
!! ATATACTGAAATAAATCTTA+AGG - tig0024533:16214-16233 Msa1417980:intron 15.0%
!! TATACTGAAATAAATCTTAA+GGG - tig0024533:16215-16234 Msa1417980:intron 15.0%
!! GAAGTATATTTCATATTAAT+AGG + tig0024533:16956-16975 None:intergenic 15.0%
!!! TAATTTTCGTAAATTATTAC+CGG - tig0024533:17415-17434 Msa1417980:intron 15.0%
!!! CATCTATTTCTTTTTTAAAA+TGG + tig0024533:17523-17542 None:intergenic 15.0%
!!! ATATTGTTATTGTAGTTTTC+AGG + tig0024533:16198-16217 None:intergenic 20.0%
!!! ACTACAATTTTCCATATTTA+AGG - tig0024533:16268-16287 Msa1417980:intron 20.0%
!!! GTTGTTTTTATAAGTTATCT+TGG - tig0024533:16386-16405 Msa1417980:intron 20.0%
!! GTTTGTAGAAATTTCATTTA+GGG + tig0024533:16539-16558 None:intergenic 20.0%
!!! ATATTGTTATTGTAGTTTTC+AGG + tig0024533:16198-16217 None:intergenic 20.0%
!!! ACTACAATTTTCCATATTTA+AGG - tig0024533:16268-16287 Msa1417980:intron 20.0%
!!! GTTGTTTTTATAAGTTATCT+TGG - tig0024533:16386-16405 Msa1417980:intron 20.0%
!! GTTTGTAGAAATTTCATTTA+GGG + tig0024533:16539-16558 None:intergenic 20.0%
!! TGATATATTGACAGTTTGTT+TGG - tig0024533:15873-15892 Msa1417980:CDS 25.0%
!! GGTTTTGCATATAAATTTTC+AGG - tig0024533:15918-15937 Msa1417980:CDS 25.0%
!!! TAAATTTTCAGGATGAGAAA+AGG - tig0024533:15929-15948 Msa1417980:CDS 25.0%
!! ATAAACTTCAAAGGTTTTCA+TGG + tig0024533:16114-16133 None:intergenic 25.0%
! GGTTTGTAGAAATTTCATTT+AGG + tig0024533:16540-16559 None:intergenic 25.0%
!! AATTGATTTAGATGCTTACA+CGG + tig0024533:16681-16700 None:intergenic 25.0%
!!! TCAATTTTCAACGTGTTATT+TGG - tig0024533:16695-16714 Msa1417980:CDS 25.0%
! TATTTGTCTGTTGAAACAAA+GGG + tig0024533:16888-16907 None:intergenic 25.0%
! ATATTTGTCTGTTGAAACAA+AGG + tig0024533:16889-16908 None:intergenic 25.0%
! AGCTAAAGATGAAGAAATTA+TGG - tig0024533:17251-17270 Msa1417980:intron 25.0%
! ATGTCATCTATATAAAGTGT+CGG + tig0024533:17319-17338 None:intergenic 25.0%
! ATGTAGTTCAGTATGTTATA+TGG + tig0024533:17631-17650 None:intergenic 25.0%
! TAATAAGTTCAGATGATAAC+AGG - tig0024533:17763-17782 Msa1417980:five_prime_UTR 25.0%
!! TGATATATTGACAGTTTGTT+TGG - tig0024533:15873-15892 Msa1417980:CDS 25.0%
!! GGTTTTGCATATAAATTTTC+AGG - tig0024533:15918-15937 Msa1417980:CDS 25.0%
!!! TAAATTTTCAGGATGAGAAA+AGG - tig0024533:15929-15948 Msa1417980:CDS 25.0%
!! ATAAACTTCAAAGGTTTTCA+TGG + tig0024533:16114-16133 None:intergenic 25.0%
! GGTTTGTAGAAATTTCATTT+AGG + tig0024533:16540-16559 None:intergenic 25.0%
!! AATTGATTTAGATGCTTACA+CGG + tig0024533:16681-16700 None:intergenic 25.0%
!!! TCAATTTTCAACGTGTTATT+TGG - tig0024533:16695-16714 Msa1417980:CDS 25.0%
! TATTTGTCTGTTGAAACAAA+GGG + tig0024533:16888-16907 None:intergenic 25.0%
! ATATTTGTCTGTTGAAACAA+AGG + tig0024533:16889-16908 None:intergenic 25.0%
! AGCTAAAGATGAAGAAATTA+TGG - tig0024533:17251-17270 Msa1417980:intron 25.0%
! ATGTCATCTATATAAAGTGT+CGG + tig0024533:17319-17338 None:intergenic 25.0%
! ATGTAGTTCAGTATGTTATA+TGG + tig0024533:17631-17650 None:intergenic 25.0%
! TAATAAGTTCAGATGATAAC+AGG - tig0024533:17763-17782 Msa1417980:five_prime_UTR 25.0%
TTTGTTTGGTTGATCTTATG+TGG - tig0024533:15887-15906 Msa1417980:CDS 30.0%
TTCACCATTTGCAATTCTAT+TGG + tig0024533:15992-16011 None:intergenic 30.0%
CAGGATCATATAAACTTCAA+AGG + tig0024533:16123-16142 None:intergenic 30.0%
! AGGGTAAGTTTAAGTTTAAG+AGG - tig0024533:16234-16253 Msa1417980:intron 30.0%
! GGGTAAGTTTAAGTTTAAGA+GGG - tig0024533:16235-16254 Msa1417980:intron 30.0%
TTGATTGGACTCCTTAAATA+TGG + tig0024533:16282-16301 None:intergenic 30.0%
TATTATAAGCGCATTAGTGT+TGG + tig0024533:16315-16334 None:intergenic 30.0%
CTACATTTGCAAAGACATAA+TGG + tig0024533:16561-16580 None:intergenic 30.0%
!! TGTTATTTGGTGTTTACTTC+AGG - tig0024533:16708-16727 Msa1417980:intron 30.0%
AAAAGTGAGACTAAAAACAC+TGG - tig0024533:16738-16757 Msa1417980:intron 30.0%
TAAAAAACAACACACACTGT+TGG - tig0024533:16908-16927 Msa1417980:CDS 30.0%
CAGAAAACATGTTGAAGAAA+AGG - tig0024533:16978-16997 Msa1417980:intron 30.0%
TGAAAAGGAAAATGAGATAC+AGG - tig0024533:17026-17045 Msa1417980:intron 30.0%
!!! TGTCAGGTTTTTTGTATGAT+AGG - tig0024533:17169-17188 Msa1417980:intron 30.0%
AAAGAGAGGTCATTAAATTG+AGG - tig0024533:17196-17215 Msa1417980:intron 30.0%
TAAATTATTACCGGTGTCTT+CGG - tig0024533:17424-17443 Msa1417980:intron 30.0%
TTTGTTTGGTTGATCTTATG+TGG - tig0024533:15887-15906 Msa1417980:CDS 30.0%
TTCACCATTTGCAATTCTAT+TGG + tig0024533:15992-16011 None:intergenic 30.0%
CAGGATCATATAAACTTCAA+AGG + tig0024533:16123-16142 None:intergenic 30.0%
! AGGGTAAGTTTAAGTTTAAG+AGG - tig0024533:16234-16253 Msa1417980:intron 30.0%
! GGGTAAGTTTAAGTTTAAGA+GGG - tig0024533:16235-16254 Msa1417980:intron 30.0%
TTGATTGGACTCCTTAAATA+TGG + tig0024533:16282-16301 None:intergenic 30.0%
TATTATAAGCGCATTAGTGT+TGG + tig0024533:16315-16334 None:intergenic 30.0%
CTACATTTGCAAAGACATAA+TGG + tig0024533:16561-16580 None:intergenic 30.0%
!! TGTTATTTGGTGTTTACTTC+AGG - tig0024533:16708-16727 Msa1417980:intron 30.0%
AAAAGTGAGACTAAAAACAC+TGG - tig0024533:16738-16757 Msa1417980:intron 30.0%
TAAAAAACAACACACACTGT+TGG - tig0024533:16908-16927 Msa1417980:CDS 30.0%
CAGAAAACATGTTGAAGAAA+AGG - tig0024533:16978-16997 Msa1417980:intron 30.0%
TGAAAAGGAAAATGAGATAC+AGG - tig0024533:17026-17045 Msa1417980:intron 30.0%
!!! TGTCAGGTTTTTTGTATGAT+AGG - tig0024533:17169-17188 Msa1417980:intron 30.0%
AAAGAGAGGTCATTAAATTG+AGG - tig0024533:17196-17215 Msa1417980:intron 30.0%
TAAATTATTACCGGTGTCTT+CGG - tig0024533:17424-17443 Msa1417980:intron 30.0%
TGATCTTATGTGGTAACTTG+TGG - tig0024533:15897-15916 Msa1417980:CDS 35.0%
! AGTATTTAAGGCATGAAAGC+AGG + tig0024533:16142-16161 None:intergenic 35.0%
AAATGTAGACAGAGATGCAA+AGG - tig0024533:16573-16592 Msa1417980:CDS 35.0%
ATAAGTTCCCCAACAGAAAA+TGG - tig0024533:16811-16830 Msa1417980:CDS 35.0%
!!! TGTGATGTGGATCTCTTTTT+TGG + tig0024533:16841-16860 None:intergenic 35.0%
AAAGGAAAATGAGATACAGG+TGG - tig0024533:17029-17048 Msa1417980:intron 35.0%
! AGTCATTTGGTTTCTTGCTT+TGG + tig0024533:17131-17150 None:intergenic 35.0%
AGCTTGCAAATTACTGTCTT+TGG + tig0024533:17235-17254 None:intergenic 35.0%
ATACATAGCTAGTGAAAGTC+GGG + tig0024533:17294-17313 None:intergenic 35.0%
GATACATAGCTAGTGAAAGT+CGG + tig0024533:17295-17314 None:intergenic 35.0%
ATTGAATGTAACCATGTGTC+AGG + tig0024533:17390-17409 None:intergenic 35.0%
AAATTATTACCGGTGTCTTC+GGG - tig0024533:17425-17444 Msa1417980:intron 35.0%
ATGTTTCACAGTCTACAAGT+AGG - tig0024533:17491-17510 Msa1417980:intron 35.0%
TGAGTTCGTTACTGTAGTTT+AGG - tig0024533:17541-17560 Msa1417980:intron 35.0%
GTTCAGTATGTTATATGGAG+AGG + tig0024533:17626-17645 None:intergenic 35.0%
TGATCTTATGTGGTAACTTG+TGG - tig0024533:15897-15916 Msa1417980:CDS 35.0%
! AGTATTTAAGGCATGAAAGC+AGG + tig0024533:16142-16161 None:intergenic 35.0%
AAATGTAGACAGAGATGCAA+AGG - tig0024533:16573-16592 Msa1417980:CDS 35.0%
ATAAGTTCCCCAACAGAAAA+TGG - tig0024533:16811-16830 Msa1417980:CDS 35.0%
!!! TGTGATGTGGATCTCTTTTT+TGG + tig0024533:16841-16860 None:intergenic 35.0%
AAAGGAAAATGAGATACAGG+TGG - tig0024533:17029-17048 Msa1417980:intron 35.0%
! AGTCATTTGGTTTCTTGCTT+TGG + tig0024533:17131-17150 None:intergenic 35.0%
AGCTTGCAAATTACTGTCTT+TGG + tig0024533:17235-17254 None:intergenic 35.0%
ATACATAGCTAGTGAAAGTC+GGG + tig0024533:17294-17313 None:intergenic 35.0%
GATACATAGCTAGTGAAAGT+CGG + tig0024533:17295-17314 None:intergenic 35.0%
ATTGAATGTAACCATGTGTC+AGG + tig0024533:17390-17409 None:intergenic 35.0%
AAATTATTACCGGTGTCTTC+GGG - tig0024533:17425-17444 Msa1417980:intron 35.0%
ATGTTTCACAGTCTACAAGT+AGG - tig0024533:17491-17510 Msa1417980:intron 35.0%
TGAGTTCGTTACTGTAGTTT+AGG - tig0024533:17541-17560 Msa1417980:intron 35.0%
GTTCAGTATGTTATATGGAG+AGG + tig0024533:17626-17645 None:intergenic 35.0%
ATGAGAAAAGGACACTAGAG+AGG - tig0024533:15941-15960 Msa1417980:CDS 40.0%
TGAGAAAAGGACACTAGAGA+GGG - tig0024533:15942-15961 Msa1417980:CDS 40.0%
GCTAGCAAGGGTAAAAGTTT+CGG - tig0024533:15966-15985 Msa1417980:CDS 40.0%
TCGGCCAATAGAATTGCAAA+TGG - tig0024533:15985-16004 Msa1417980:CDS 40.0%
CAAATGGTGAAGCTAATGAG+TGG - tig0024533:16001-16020 Msa1417980:CDS 40.0%
GTGGAAGGATGAAAATGACA+AGG - tig0024533:16020-16039 Msa1417980:intron 40.0%
AGAAGAGAGAAGGATTATGC+AGG - tig0024533:16065-16084 Msa1417980:intron 40.0%
TTAAGGAGTCCAATCAAGAG+AGG - tig0024533:16285-16304 Msa1417980:intron 40.0%
TATATCAGAGAGAACAGCCA+AGG - tig0024533:16613-16632 Msa1417980:CDS 40.0%
AGTGAGACTAAAAACACTGG+AGG - tig0024533:16741-16760 Msa1417980:intron 40.0%
AGACTAAAAACACTGGAGGA+AGG - tig0024533:16745-16764 Msa1417980:intron 40.0%
! TTGGACCACCATTTTCTGTT+GGG + tig0024533:16822-16841 None:intergenic 40.0%
! TTTGGACCACCATTTTCTGT+TGG + tig0024533:16823-16842 None:intergenic 40.0%
AAAACAACACACACTGTTGG+AGG - tig0024533:16911-16930 Msa1417980:CDS 40.0%
AAACAACACACACTGTTGGA+GGG - tig0024533:16912-16931 Msa1417980:CDS 40.0%
TGTTGGAGGGAATTCAAAAC+AGG - tig0024533:16925-16944 Msa1417980:CDS 40.0%
CCACTATCAGCAACTTTACT+TGG + tig0024533:17006-17025 None:intergenic 40.0%
CCAAGTAAAGTTGCTGATAG+TGG - tig0024533:17003-17022 Msa1417980:intron 40.0%
!! AGTTGCTGATAGTGGTGAAA+AGG - tig0024533:17011-17030 Msa1417980:intron 40.0%
! CTTCACTTTCAGCATTGAAC+TGG + tig0024533:17074-17093 None:intergenic 40.0%
ACACGTGTCAAACATTGACA+CGG + tig0024533:17345-17364 None:intergenic 40.0%
GTCAGTATCAGAAACCAACA+CGG - tig0024533:17363-17382 Msa1417980:intron 40.0%
! GGGTTAGTGTCAATGTTGTT+TGG - tig0024533:17445-17464 Msa1417980:intron 40.0%
GAAACAAAGACTCCTCCAAA+AGG - tig0024533:17793-17812 Msa1417980:five_prime_UTR 40.0%
! TATCAGCCACACTAACCTTT+TGG + tig0024533:17811-17830 None:intergenic 40.0%
ATGAGAAAAGGACACTAGAG+AGG - tig0024533:15941-15960 Msa1417980:CDS 40.0%
TGAGAAAAGGACACTAGAGA+GGG - tig0024533:15942-15961 Msa1417980:CDS 40.0%
GCTAGCAAGGGTAAAAGTTT+CGG - tig0024533:15966-15985 Msa1417980:CDS 40.0%
TCGGCCAATAGAATTGCAAA+TGG - tig0024533:15985-16004 Msa1417980:CDS 40.0%
CAAATGGTGAAGCTAATGAG+TGG - tig0024533:16001-16020 Msa1417980:CDS 40.0%
GTGGAAGGATGAAAATGACA+AGG - tig0024533:16020-16039 Msa1417980:intron 40.0%
AGAAGAGAGAAGGATTATGC+AGG - tig0024533:16065-16084 Msa1417980:intron 40.0%
TTAAGGAGTCCAATCAAGAG+AGG - tig0024533:16285-16304 Msa1417980:intron 40.0%
TATATCAGAGAGAACAGCCA+AGG - tig0024533:16613-16632 Msa1417980:CDS 40.0%
AGTGAGACTAAAAACACTGG+AGG - tig0024533:16741-16760 Msa1417980:intron 40.0%
AGACTAAAAACACTGGAGGA+AGG - tig0024533:16745-16764 Msa1417980:intron 40.0%
! TTGGACCACCATTTTCTGTT+GGG + tig0024533:16822-16841 None:intergenic 40.0%
! TTTGGACCACCATTTTCTGT+TGG + tig0024533:16823-16842 None:intergenic 40.0%
AAAACAACACACACTGTTGG+AGG - tig0024533:16911-16930 Msa1417980:CDS 40.0%
AAACAACACACACTGTTGGA+GGG - tig0024533:16912-16931 Msa1417980:CDS 40.0%
TGTTGGAGGGAATTCAAAAC+AGG - tig0024533:16925-16944 Msa1417980:CDS 40.0%
CCACTATCAGCAACTTTACT+TGG + tig0024533:17006-17025 None:intergenic 40.0%
CCAAGTAAAGTTGCTGATAG+TGG - tig0024533:17003-17022 Msa1417980:intron 40.0%
!! AGTTGCTGATAGTGGTGAAA+AGG - tig0024533:17011-17030 Msa1417980:intron 40.0%
! CTTCACTTTCAGCATTGAAC+TGG + tig0024533:17074-17093 None:intergenic 40.0%
ACACGTGTCAAACATTGACA+CGG + tig0024533:17345-17364 None:intergenic 40.0%
GTCAGTATCAGAAACCAACA+CGG - tig0024533:17363-17382 Msa1417980:intron 40.0%
! GGGTTAGTGTCAATGTTGTT+TGG - tig0024533:17445-17464 Msa1417980:intron 40.0%
GAAACAAAGACTCCTCCAAA+AGG - tig0024533:17793-17812 Msa1417980:five_prime_UTR 40.0%
! TATCAGCCACACTAACCTTT+TGG + tig0024533:17811-17830 None:intergenic 40.0%
TGGTGAAGCTAATGAGTGGA+AGG - tig0024533:16005-16024 Msa1417980:CDS 45.0%
TCTTCTAGCCATTGCTTGAC+AGG + tig0024533:16051-16070 None:intergenic 45.0%
AGCAATGGCTAGAAGAGAGA+AGG - tig0024533:16055-16074 Msa1417980:intron 45.0%
CTTCATCTGTGATTCTGCCT+TGG + tig0024533:16633-16652 None:intergenic 45.0%
CAAGGCAGAATCACAGATGA+AGG - tig0024533:16631-16650 Msa1417980:CDS 45.0%
AGTTCCCCAACAGAAAATGG+TGG - tig0024533:16814-16833 Msa1417980:CDS 45.0%
! TGGACCACCATTTTCTGTTG+GGG + tig0024533:16821-16840 None:intergenic 45.0%
AGAAGCTCTTGGTTGTGATG+TGG + tig0024533:16854-16873 None:intergenic 45.0%
GTATGATAGGCTGCAAAGAG+AGG - tig0024533:17182-17201 Msa1417980:intron 45.0%
CACGTGTCAAACATTGACAC+GGG + tig0024533:17344-17363 None:intergenic 45.0%
TTTCTTCCGCTCTACTTCCA+TGG + tig0024533:17700-17719 None:intergenic 45.0%
AGATGCAGCTGCTAGAGAAA+AGG - tig0024533:17728-17747 Msa1417980:five_prime_UTR 45.0%
ACTCCTCCAAAAGGTTAGTG+TGG - tig0024533:17802-17821 Msa1417980:five_prime_UTR 45.0%
TGGTGAAGCTAATGAGTGGA+AGG - tig0024533:16005-16024 Msa1417980:CDS 45.0%
TCTTCTAGCCATTGCTTGAC+AGG + tig0024533:16051-16070 None:intergenic 45.0%
AGCAATGGCTAGAAGAGAGA+AGG - tig0024533:16055-16074 Msa1417980:intron 45.0%
CTTCATCTGTGATTCTGCCT+TGG + tig0024533:16633-16652 None:intergenic 45.0%
CAAGGCAGAATCACAGATGA+AGG - tig0024533:16631-16650 Msa1417980:CDS 45.0%
AGTTCCCCAACAGAAAATGG+TGG - tig0024533:16814-16833 Msa1417980:CDS 45.0%
! TGGACCACCATTTTCTGTTG+GGG + tig0024533:16821-16840 None:intergenic 45.0%
AGAAGCTCTTGGTTGTGATG+TGG + tig0024533:16854-16873 None:intergenic 45.0%
GTATGATAGGCTGCAAAGAG+AGG - tig0024533:17182-17201 Msa1417980:intron 45.0%
CACGTGTCAAACATTGACAC+GGG + tig0024533:17344-17363 None:intergenic 45.0%
TTTCTTCCGCTCTACTTCCA+TGG + tig0024533:17700-17719 None:intergenic 45.0%
AGATGCAGCTGCTAGAGAAA+AGG - tig0024533:17728-17747 Msa1417980:five_prime_UTR 45.0%
ACTCCTCCAAAAGGTTAGTG+TGG - tig0024533:17802-17821 Msa1417980:five_prime_UTR 45.0%
ACTAGAGAGGGAGCTAGCAA+GGG - tig0024533:15954-15973 Msa1417980:CDS 50.0%
AGGTCATGCCTGTCAAGCAA+TGG - tig0024533:16040-16059 Msa1417980:intron 50.0%
GTTGGACAGCCTCTCTTGAT+TGG + tig0024533:16297-16316 None:intergenic 50.0%
CAACCAAGAGCTTCTGCGAT+TGG - tig0024533:16859-16878 Msa1417980:CDS 50.0%
CTTCCAATCGCAGAAGCTCT+TGG + tig0024533:16865-16884 None:intergenic 50.0%
TCGTCACTGCACGAGTCATT+TGG + tig0024533:17144-17163 None:intergenic 50.0%
TGACTCGTGCAGTGACGATT+TGG - tig0024533:17146-17165 Msa1417980:intron 50.0%
!! TGCAGTGACGATTTGGTGTC+AGG - tig0024533:17153-17172 Msa1417980:intron 50.0%
TGACACTAACCCGAAGACAC+CGG + tig0024533:17437-17456 None:intergenic 50.0%
! TGATGCACTGACCAAGTCCA+TGG - tig0024533:17680-17699 Msa1417980:five_prime_UTR 50.0%
CCAAGTCCATGGAAGTAGAG+CGG - tig0024533:17691-17710 Msa1417980:five_prime_UTR 50.0%
TGCAGCTGCTAGAGAAAAGG+AGG - tig0024533:17731-17750 Msa1417980:five_prime_UTR 50.0%
! CAGCCACACTAACCTTTTGG+AGG + tig0024533:17808-17827 None:intergenic 50.0%
ACTAGAGAGGGAGCTAGCAA+GGG - tig0024533:15954-15973 Msa1417980:CDS 50.0%
AGGTCATGCCTGTCAAGCAA+TGG - tig0024533:16040-16059 Msa1417980:intron 50.0%
GTTGGACAGCCTCTCTTGAT+TGG + tig0024533:16297-16316 None:intergenic 50.0%
CAACCAAGAGCTTCTGCGAT+TGG - tig0024533:16859-16878 Msa1417980:CDS 50.0%
CTTCCAATCGCAGAAGCTCT+TGG + tig0024533:16865-16884 None:intergenic 50.0%
TCGTCACTGCACGAGTCATT+TGG + tig0024533:17144-17163 None:intergenic 50.0%
TGACTCGTGCAGTGACGATT+TGG - tig0024533:17146-17165 Msa1417980:intron 50.0%
!! TGCAGTGACGATTTGGTGTC+AGG - tig0024533:17153-17172 Msa1417980:intron 50.0%
TGACACTAACCCGAAGACAC+CGG + tig0024533:17437-17456 None:intergenic 50.0%
! TGATGCACTGACCAAGTCCA+TGG - tig0024533:17680-17699 Msa1417980:five_prime_UTR 50.0%
CCAAGTCCATGGAAGTAGAG+CGG - tig0024533:17691-17710 Msa1417980:five_prime_UTR 50.0%
TGCAGCTGCTAGAGAAAAGG+AGG - tig0024533:17731-17750 Msa1417980:five_prime_UTR 50.0%
! CAGCCACACTAACCTTTTGG+AGG + tig0024533:17808-17827 None:intergenic 50.0%
CACTAGAGAGGGAGCTAGCA+AGG - tig0024533:15953-15972 Msa1417980:CDS 55.0%
ACCAACACGGTCCTGACACA+TGG - tig0024533:17376-17395 Msa1417980:intron 55.0%
ACCATGTGTCAGGACCGTGT+TGG + tig0024533:17380-17399 None:intergenic 55.0%
CCGCTCTACTTCCATGGACT+TGG + tig0024533:17694-17713 None:intergenic 55.0%
CACTAGAGAGGGAGCTAGCA+AGG - tig0024533:15953-15972 Msa1417980:CDS 55.0%
ACCAACACGGTCCTGACACA+TGG - tig0024533:17376-17395 Msa1417980:intron 55.0%
ACCATGTGTCAGGACCGTGT+TGG + tig0024533:17380-17399 None:intergenic 55.0%
CCGCTCTACTTCCATGGACT+TGG + tig0024533:17694-17713 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0024533 gene 15828 17872 15828 ID=Msa1417980;Name=Msa1417980
tig0024533 mRNA 15828 17872 15828 ID=Msa1417980-mRNA-1;Parent=Msa1417980;Name=Msa1417980-mRNA-1;_AED=0.33;_eAED=0.33;_QI=217|0|0.25|1|1|1|4|0|207
tig0024533 exon 15828 16025 15828 ID=Msa1417980-mRNA-1:exon:173;Parent=Msa1417980-mRNA-1
tig0024533 exon 16497 16719 16497 ID=Msa1417980-mRNA-1:exon:172;Parent=Msa1417980-mRNA-1
tig0024533 exon 16810 16970 16810 ID=Msa1417980-mRNA-1:exon:171;Parent=Msa1417980-mRNA-1
tig0024533 exon 17614 17872 17614 ID=Msa1417980-mRNA-1:exon:170;Parent=Msa1417980-mRNA-1
tig0024533 five_prime_UTR 17656 17872 17656 ID=Msa1417980-mRNA-1:five_prime_utr;Parent=Msa1417980-mRNA-1
tig0024533 CDS 17614 17655 17614 ID=Msa1417980-mRNA-1:cds;Parent=Msa1417980-mRNA-1
tig0024533 CDS 16810 16970 16810 ID=Msa1417980-mRNA-1:cds;Parent=Msa1417980-mRNA-1
tig0024533 CDS 16497 16719 16497 ID=Msa1417980-mRNA-1:cds;Parent=Msa1417980-mRNA-1
tig0024533 CDS 15828 16025 15828 ID=Msa1417980-mRNA-1:cds;Parent=Msa1417980-mRNA-1
Gene Sequence

>Msa1417980

ATGCCTGTCAAGCAATGGCTAGAAGAGAGAAGGATTATGCAGGAAAAGCTAAAAGTGAGACTAAAAACACTGGAGGAAGGTTTAAAACATTCTTCAAGTTCAAAAGCAGAAAAGTCTAACATCATAAGTTCCCCAACAGAAAATGGTGGTCCAAAAAAGAGATCCACATCACAACCAAGAGCTTCTGCGATTGGAAGCCCTTTAAAACATGTTGAAGAAAAGGATCCAAGTAAAGTTGCTGATAGTGGTGAAAAGGAAAATGAGATACAGGTGGACACAGACATGAATTTAAACCAGTTCAATGCTGAAAGTGAAGAAATCAAAACAATTGTTGATGTGAATGAAGACTTCCAAAGCAAGAAACCAAATGACTCGTGCAGTGACGATTTGGTGTCAGGTTTTTTGTATGATAGGCTGCAAAGAGAGAAAGTTGATGCACTGACCAAGTCCATGGAAGTAGAGCGGAAGAAAATGAAAAGAGATGCAGCTGCTAGAGAAAAGGAGGTTTCAGCAATAATAAGTTCAGATGATAACAGGAAAAACAGAAACAAAGACTCCTCCAAAAGGTTAGTGTGGCTGATACATGCTAACAAGTTTGTTCTGTTTGTCTTAACTATAGCCTAA
Protein sequence

>Msa1417980

MPVKQWLEERRIMQEKLKVRLKTLEEGLKHSSSSKAEKSNIISSPTENGGPKKRSTSQPRASAIGSPLKHVEEKDPSKVADSGEKENEIQVDTDMNLNQFNAESEEIKTIVDVNEDFQSKKPNDSCSDDLVSGFLYDRLQREKVDALTKSMEVERKKMKRDAAAREKEVSAIISSDDNRKNRNKDSSKRLVWLIHANKFVLFVLTIA*