AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1443880

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1443880 MtrunA17_Chr4g0076251 66.798 253 22 4 7 203 324 570 1.16e-98 297
Msa1443880 MtrunA17_Chr2g0290411 43.701 254 73 8 6 203 310 549 2.69e-34 128
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1443880 AT4G17220.1 33.488 215 68 6 7 180 304 484 2.69e-18 83.2

Find 50 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACTATCAGCAACTTTACT+TGG 0.264053 tig0030573:-45041 Msa1443880:intergenic
TTCATCTGTGATTCTGCCTT+TGG 0.290781 tig0030573:-44667 Msa1443880:intergenic
ATAAGTTCCCCAACAGAAAA+TGG 0.325320 tig0030573:+44849 Msa1443880:CDS
TTCACCATTTGCAATTCTAT+TGG 0.325874 tig0030573:-44026 Msa1443880:intergenic
TATCAGCCACACTAACCTTT+TGG 0.326069 tig0030573:-45846 Msa1443880:intergenic
AGTCATTTGGTTTCTTGCTT+TGG 0.339262 tig0030573:-45166 Msa1443880:intergenic
TGCAGTGACGATTTGGTGTC+AGG 0.351949 tig0030573:+45191 Msa1443880:CDS
TGATATATTGACAGTTTGTT+TGG 0.367532 tig0030573:+43910 Msa1443880:five_prime_UTR
CTTCACTTTCAGCATTGAAC+TGG 0.389403 tig0030573:-45109 Msa1443880:intergenic
TAATAAGTTCAGATGATAAC+AGG 0.405133 tig0030573:+45801 Msa1443880:CDS
CAGAAAACATGTTGAAGAAA+AGG 0.410590 tig0030573:+45016 Msa1443880:intron
GCTAGCAAGGGTAAAAGTTT+CGG 0.430627 tig0030573:+44003 Msa1443880:five_prime_UTR
TCGTCACTGCACGAGTCATT+TGG 0.432134 tig0030573:-45179 Msa1443880:intergenic
TGAAAAGGAAAATGAGATAC+AGG 0.451771 tig0030573:+45064 Msa1443880:CDS
AGTTGCTGATAGTGGTGAAA+AGG 0.454137 tig0030573:+45049 Msa1443880:CDS
TTTCTTCCGCTCTACTTCCA+TGG 0.458047 tig0030573:-45735 Msa1443880:intergenic
TGACTCGTGCAGTGACGATT+TGG 0.459803 tig0030573:+45184 Msa1443880:CDS
CTTCCAATCGCAGAAGCTCT+TGG 0.464587 tig0030573:-44900 Msa1443880:intergenic
CAACCAAGAGCTTCTGCGAT+TGG 0.480093 tig0030573:+44897 Msa1443880:CDS
CACTAGAGAGGGAGCTAGCA+AGG 0.481581 tig0030573:+43990 Msa1443880:five_prime_UTR
CCAAGTAAAGTTGCTGATAG+TGG 0.491571 tig0030573:+45041 Msa1443880:CDS
TTTGTTTGGTTGATCTTATG+TGG 0.496728 tig0030573:+43924 Msa1443880:five_prime_UTR
TCTTCTAGCCATTACTTGAC+AGG 0.499932 tig0030573:-44085 Msa1443880:intergenic
TGTTGGAGGGAATTCAAAAC+AGG 0.509579 tig0030573:+44963 Msa1443880:CDS
ATATTTGTCTGTTGAAACAA+AGG 0.515307 tig0030573:-44924 Msa1443880:intergenic
TATTTGTCTGTTGAAACAAA+GGG 0.522956 tig0030573:-44923 Msa1443880:intergenic
AAAACAACACACACTGTTGG+AGG 0.531374 tig0030573:+44949 Msa1443880:CDS
AGATGCAGCTGCTAGAGAAA+AGG 0.532223 tig0030573:+45766 Msa1443880:CDS
GAAACAAAGACTCCTCCAAA+AGG 0.543913 tig0030573:+45831 Msa1443880:CDS
TCGGCCAATAGAATTGCAAA+TGG 0.544219 tig0030573:+44022 Msa1443880:five_prime_UTR
AAAGGCAGAATCACAGATGA+AGG 0.546970 tig0030573:+44669 Msa1443880:CDS
TGATCTTATGTGGTAACTTG+TGG 0.547591 tig0030573:+43934 Msa1443880:five_prime_UTR
AGAAGAGAGAAGGATTATGC+AGG 0.567503 tig0030573:+44102 Msa1443880:exon
TGCAGCTGCTAGAGAAAAGG+AGG 0.568762 tig0030573:+45769 Msa1443880:CDS
AAACAACACACACTGTTGGA+GGG 0.570185 tig0030573:+44950 Msa1443880:CDS
AGTAATGGCTAGAAGAGAGA+AGG 0.579688 tig0030573:+44092 Msa1443880:five_prime_UTR
AGGTCATGCCTGTCAAGTAA+TGG 0.579913 tig0030573:+44077 Msa1443880:five_prime_UTR
ATGAGAAAAGGACACTAGAG+AGG 0.607096 tig0030573:+43978 Msa1443880:five_prime_UTR
GTATGATAGGCTGCAAAGAG+AGG 0.608475 tig0030573:+45220 Msa1443880:CDS
ACTCCTCCAAAAGGTTAGTG+TGG 0.610939 tig0030573:+45840 Msa1443880:CDS
ACTAGAGAGGGAGCTAGCAA+GGG 0.615439 tig0030573:+43991 Msa1443880:five_prime_UTR
TGGTGAAGCTAATGAGTGGA+AGG 0.633476 tig0030573:+44042 Msa1443880:five_prime_UTR
TGAGAAAAGGACACTAGAGA+GGG 0.660251 tig0030573:+43979 Msa1443880:five_prime_UTR
ATATCAGAGAGAACAGCCAA+AGG 0.666354 tig0030573:+44651 Msa1443880:intron
AAAGGAAAATGAGATACAGG+TGG 0.667770 tig0030573:+45067 Msa1443880:CDS
AGAAGCTCTTGGTTGTGATG+TGG 0.668929 tig0030573:-44889 Msa1443880:intergenic
CCAAGTCCATGGAAGTAGAG+CGG 0.682402 tig0030573:+45729 Msa1443880:intron
GTGGAAGGATGAAAATGACA+AGG 0.684047 tig0030573:+44057 Msa1443880:five_prime_UTR
CAAATGGTGAAGCTAATGAG+TGG 0.695992 tig0030573:+44038 Msa1443880:five_prime_UTR
AGTTCCCCAACAGAAAATGG+TGG 0.700541 tig0030573:+44852 Msa1443880:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAAATTGTAGTATTTA+AGG - tig0030573:44191-44210 Msa1443880:intergenic 10.0%
!! AAATAAAATTGTAGTATTTA+AGG - tig0030573:44191-44210 Msa1443880:intergenic 10.0%
!!! ATTTTATTTTGTTACTTATG+AGG + tig0030573:44202-44221 Msa1443880:intron 15.0%
!! ATATACTGAAATAAATCTTA+AGG + tig0030573:44251-44270 Msa1443880:intron 15.0%
!! TATACTGAAATAAATCTTAA+GGG + tig0030573:44252-44271 Msa1443880:intron 15.0%
!! GAAGTATATTTCATATTAAT+AGG - tig0030573:44994-45013 Msa1443880:intergenic 15.0%
!!! TAATTTTCGTAAATTATTAC+CGG + tig0030573:45453-45472 Msa1443880:intron 15.0%
!!! CATCTATTTCTTTTTTAAAA+TGG - tig0030573:45561-45580 Msa1443880:intergenic 15.0%
!!! ATTTTATTTTGTTACTTATG+AGG + tig0030573:44202-44221 Msa1443880:intron 15.0%
!! ATATACTGAAATAAATCTTA+AGG + tig0030573:44251-44270 Msa1443880:intron 15.0%
!! TATACTGAAATAAATCTTAA+GGG + tig0030573:44252-44271 Msa1443880:intron 15.0%
!! GAAGTATATTTCATATTAAT+AGG - tig0030573:44994-45013 Msa1443880:intergenic 15.0%
!!! TAATTTTCGTAAATTATTAC+CGG + tig0030573:45453-45472 Msa1443880:intron 15.0%
!!! CATCTATTTCTTTTTTAAAA+TGG - tig0030573:45561-45580 Msa1443880:intergenic 15.0%
!!! ATATTGTTATTGTAGTTTTC+AGG - tig0030573:44235-44254 Msa1443880:intergenic 20.0%
!!! ACTACAATTTTCCATATTTA+AGG + tig0030573:44305-44324 Msa1443880:intron 20.0%
!!! GTTGTTTTTATAAGTTATCT+TGG + tig0030573:44423-44442 Msa1443880:intron 20.0%
!! GTTTGTAGAAATTTCATTTA+GGG - tig0030573:44576-44595 Msa1443880:intergenic 20.0%
!!! ATATTGTTATTGTAGTTTTC+AGG - tig0030573:44235-44254 Msa1443880:intergenic 20.0%
!!! ACTACAATTTTCCATATTTA+AGG + tig0030573:44305-44324 Msa1443880:intron 20.0%
!!! GTTGTTTTTATAAGTTATCT+TGG + tig0030573:44423-44442 Msa1443880:intron 20.0%
!! GTTTGTAGAAATTTCATTTA+GGG - tig0030573:44576-44595 Msa1443880:intergenic 20.0%
!! TGATATATTGACAGTTTGTT+TGG + tig0030573:43910-43929 Msa1443880:five_prime_UTR 25.0%
!! GGTTTTGCATATAAATTTTC+AGG + tig0030573:43955-43974 Msa1443880:five_prime_UTR 25.0%
!!! TAAATTTTCAGGATGAGAAA+AGG + tig0030573:43966-43985 Msa1443880:five_prime_UTR 25.0%
!! ATAAACTTCAAAGGTTTTCA+TGG - tig0030573:44151-44170 Msa1443880:intergenic 25.0%
! GGTTTGTAGAAATTTCATTT+AGG - tig0030573:44577-44596 Msa1443880:intergenic 25.0%
!! AATTGATTTAGATGCTTACA+CGG - tig0030573:44719-44738 Msa1443880:intergenic 25.0%
!!! TCAATTTTCAACGTGTTATT+TGG + tig0030573:44733-44752 Msa1443880:intron 25.0%
! TATTTGTCTGTTGAAACAAA+GGG - tig0030573:44926-44945 Msa1443880:intergenic 25.0%
! ATATTTGTCTGTTGAAACAA+AGG - tig0030573:44927-44946 Msa1443880:intergenic 25.0%
! AGCTAAAGATGAAGAAATTA+TGG + tig0030573:45289-45308 Msa1443880:intron 25.0%
! ATGTCATCTATATAAAGTGT+CGG - tig0030573:45357-45376 Msa1443880:intergenic 25.0%
! ATGTAGTTCAGTATGTTATA+TGG - tig0030573:45669-45688 Msa1443880:intergenic 25.0%
! TAATAAGTTCAGATGATAAC+AGG + tig0030573:45801-45820 Msa1443880:CDS 25.0%
!! TGATATATTGACAGTTTGTT+TGG + tig0030573:43910-43929 Msa1443880:five_prime_UTR 25.0%
!! GGTTTTGCATATAAATTTTC+AGG + tig0030573:43955-43974 Msa1443880:five_prime_UTR 25.0%
!!! TAAATTTTCAGGATGAGAAA+AGG + tig0030573:43966-43985 Msa1443880:five_prime_UTR 25.0%
!! ATAAACTTCAAAGGTTTTCA+TGG - tig0030573:44151-44170 Msa1443880:intergenic 25.0%
! GGTTTGTAGAAATTTCATTT+AGG - tig0030573:44577-44596 Msa1443880:intergenic 25.0%
!! AATTGATTTAGATGCTTACA+CGG - tig0030573:44719-44738 Msa1443880:intergenic 25.0%
!!! TCAATTTTCAACGTGTTATT+TGG + tig0030573:44733-44752 Msa1443880:intron 25.0%
! TATTTGTCTGTTGAAACAAA+GGG - tig0030573:44926-44945 Msa1443880:intergenic 25.0%
! ATATTTGTCTGTTGAAACAA+AGG - tig0030573:44927-44946 Msa1443880:intergenic 25.0%
! AGCTAAAGATGAAGAAATTA+TGG + tig0030573:45289-45308 Msa1443880:intron 25.0%
! ATGTCATCTATATAAAGTGT+CGG - tig0030573:45357-45376 Msa1443880:intergenic 25.0%
! ATGTAGTTCAGTATGTTATA+TGG - tig0030573:45669-45688 Msa1443880:intergenic 25.0%
! TAATAAGTTCAGATGATAAC+AGG + tig0030573:45801-45820 Msa1443880:CDS 25.0%
TTTGTTTGGTTGATCTTATG+TGG + tig0030573:43924-43943 Msa1443880:five_prime_UTR 30.0%
TTCACCATTTGCAATTCTAT+TGG - tig0030573:44029-44048 Msa1443880:intergenic 30.0%
CAGGATCATATAAACTTCAA+AGG - tig0030573:44160-44179 Msa1443880:intergenic 30.0%
! AGGGTAAGTTTAAGTTTAAG+AGG + tig0030573:44271-44290 Msa1443880:intron 30.0%
! GGGTAAGTTTAAGTTTAAGA+GGG + tig0030573:44272-44291 Msa1443880:intron 30.0%
TTGATTGGACTCCTTAAATA+TGG - tig0030573:44319-44338 Msa1443880:intergenic 30.0%
TATTATAAGCGCATTAGTGT+TGG - tig0030573:44352-44371 Msa1443880:intergenic 30.0%
CTACATTTGCAAAGACATAA+TGG - tig0030573:44598-44617 Msa1443880:intergenic 30.0%
!! TGTTATTTGGTGTTTACTTC+AGG + tig0030573:44746-44765 Msa1443880:intron 30.0%
AAAAGTGAGACTAAAAACAC+TGG + tig0030573:44776-44795 Msa1443880:CDS 30.0%
TAAAAAACAACACACACTGT+TGG + tig0030573:44946-44965 Msa1443880:CDS 30.0%
CAGAAAACATGTTGAAGAAA+AGG + tig0030573:45016-45035 Msa1443880:intron 30.0%
TGAAAAGGAAAATGAGATAC+AGG + tig0030573:45064-45083 Msa1443880:CDS 30.0%
!!! TGTCAGGTTTTTTGTATGAT+AGG + tig0030573:45207-45226 Msa1443880:CDS 30.0%
AAAGAGAGGTCATTAAATTG+AGG + tig0030573:45234-45253 Msa1443880:intron 30.0%
TAAATTATTACCGGTGTCTT+CGG + tig0030573:45462-45481 Msa1443880:intron 30.0%
TTTGTTTGGTTGATCTTATG+TGG + tig0030573:43924-43943 Msa1443880:five_prime_UTR 30.0%
TTCACCATTTGCAATTCTAT+TGG - tig0030573:44029-44048 Msa1443880:intergenic 30.0%
CAGGATCATATAAACTTCAA+AGG - tig0030573:44160-44179 Msa1443880:intergenic 30.0%
! AGGGTAAGTTTAAGTTTAAG+AGG + tig0030573:44271-44290 Msa1443880:intron 30.0%
! GGGTAAGTTTAAGTTTAAGA+GGG + tig0030573:44272-44291 Msa1443880:intron 30.0%
TTGATTGGACTCCTTAAATA+TGG - tig0030573:44319-44338 Msa1443880:intergenic 30.0%
TATTATAAGCGCATTAGTGT+TGG - tig0030573:44352-44371 Msa1443880:intergenic 30.0%
CTACATTTGCAAAGACATAA+TGG - tig0030573:44598-44617 Msa1443880:intergenic 30.0%
!! TGTTATTTGGTGTTTACTTC+AGG + tig0030573:44746-44765 Msa1443880:intron 30.0%
AAAAGTGAGACTAAAAACAC+TGG + tig0030573:44776-44795 Msa1443880:CDS 30.0%
TAAAAAACAACACACACTGT+TGG + tig0030573:44946-44965 Msa1443880:CDS 30.0%
CAGAAAACATGTTGAAGAAA+AGG + tig0030573:45016-45035 Msa1443880:intron 30.0%
TGAAAAGGAAAATGAGATAC+AGG + tig0030573:45064-45083 Msa1443880:CDS 30.0%
!!! TGTCAGGTTTTTTGTATGAT+AGG + tig0030573:45207-45226 Msa1443880:CDS 30.0%
AAAGAGAGGTCATTAAATTG+AGG + tig0030573:45234-45253 Msa1443880:intron 30.0%
TAAATTATTACCGGTGTCTT+CGG + tig0030573:45462-45481 Msa1443880:intron 30.0%
TGATCTTATGTGGTAACTTG+TGG + tig0030573:43934-43953 Msa1443880:five_prime_UTR 35.0%
! AGTATTTAAGGCATGAAAGC+AGG - tig0030573:44179-44198 Msa1443880:intergenic 35.0%
AAATGTAGACAGAGATGCAA+AGG + tig0030573:44610-44629 Msa1443880:intron 35.0%
ATAAGTTCCCCAACAGAAAA+TGG + tig0030573:44849-44868 Msa1443880:CDS 35.0%
!!! TGTGATGTGGATCTCTTTTT+TGG - tig0030573:44879-44898 Msa1443880:intergenic 35.0%
AAAGGAAAATGAGATACAGG+TGG + tig0030573:45067-45086 Msa1443880:CDS 35.0%
! AGTCATTTGGTTTCTTGCTT+TGG - tig0030573:45169-45188 Msa1443880:intergenic 35.0%
AGCTTGCAAATTACTGTCTT+TGG - tig0030573:45273-45292 Msa1443880:intergenic 35.0%
ATACATAGCTAGTGAAAGTC+GGG - tig0030573:45332-45351 Msa1443880:intergenic 35.0%
GATACATAGCTAGTGAAAGT+CGG - tig0030573:45333-45352 Msa1443880:intergenic 35.0%
ATTGAATGTAACCATGTGTC+AGG - tig0030573:45428-45447 Msa1443880:intergenic 35.0%
AAATTATTACCGGTGTCTTC+GGG + tig0030573:45463-45482 Msa1443880:intron 35.0%
ATGTTTCACAGTCTACAAGT+AGG + tig0030573:45529-45548 Msa1443880:intron 35.0%
TGAGTTCGTTACTGTAGTTT+AGG + tig0030573:45579-45598 Msa1443880:intron 35.0%
GTTCAGTATGTTATATGGAG+AGG - tig0030573:45664-45683 Msa1443880:intergenic 35.0%
TGATCTTATGTGGTAACTTG+TGG + tig0030573:43934-43953 Msa1443880:five_prime_UTR 35.0%
! AGTATTTAAGGCATGAAAGC+AGG - tig0030573:44179-44198 Msa1443880:intergenic 35.0%
AAATGTAGACAGAGATGCAA+AGG + tig0030573:44610-44629 Msa1443880:intron 35.0%
ATAAGTTCCCCAACAGAAAA+TGG + tig0030573:44849-44868 Msa1443880:CDS 35.0%
!!! TGTGATGTGGATCTCTTTTT+TGG - tig0030573:44879-44898 Msa1443880:intergenic 35.0%
AAAGGAAAATGAGATACAGG+TGG + tig0030573:45067-45086 Msa1443880:CDS 35.0%
! AGTCATTTGGTTTCTTGCTT+TGG - tig0030573:45169-45188 Msa1443880:intergenic 35.0%
AGCTTGCAAATTACTGTCTT+TGG - tig0030573:45273-45292 Msa1443880:intergenic 35.0%
ATACATAGCTAGTGAAAGTC+GGG - tig0030573:45332-45351 Msa1443880:intergenic 35.0%
GATACATAGCTAGTGAAAGT+CGG - tig0030573:45333-45352 Msa1443880:intergenic 35.0%
ATTGAATGTAACCATGTGTC+AGG - tig0030573:45428-45447 Msa1443880:intergenic 35.0%
AAATTATTACCGGTGTCTTC+GGG + tig0030573:45463-45482 Msa1443880:intron 35.0%
ATGTTTCACAGTCTACAAGT+AGG + tig0030573:45529-45548 Msa1443880:intron 35.0%
TGAGTTCGTTACTGTAGTTT+AGG + tig0030573:45579-45598 Msa1443880:intron 35.0%
GTTCAGTATGTTATATGGAG+AGG - tig0030573:45664-45683 Msa1443880:intergenic 35.0%
ATGAGAAAAGGACACTAGAG+AGG + tig0030573:43978-43997 Msa1443880:five_prime_UTR 40.0%
TGAGAAAAGGACACTAGAGA+GGG + tig0030573:43979-43998 Msa1443880:five_prime_UTR 40.0%
GCTAGCAAGGGTAAAAGTTT+CGG + tig0030573:44003-44022 Msa1443880:five_prime_UTR 40.0%
TCGGCCAATAGAATTGCAAA+TGG + tig0030573:44022-44041 Msa1443880:five_prime_UTR 40.0%
CAAATGGTGAAGCTAATGAG+TGG + tig0030573:44038-44057 Msa1443880:five_prime_UTR 40.0%
GTGGAAGGATGAAAATGACA+AGG + tig0030573:44057-44076 Msa1443880:five_prime_UTR 40.0%
TCTTCTAGCCATTACTTGAC+AGG - tig0030573:44088-44107 Msa1443880:intergenic 40.0%
AGTAATGGCTAGAAGAGAGA+AGG + tig0030573:44092-44111 Msa1443880:five_prime_UTR 40.0%
AGAAGAGAGAAGGATTATGC+AGG + tig0030573:44102-44121 Msa1443880:exon 40.0%
TTAAGGAGTCCAATCAAGAG+AGG + tig0030573:44322-44341 Msa1443880:intron 40.0%
ATATCAGAGAGAACAGCCAA+AGG + tig0030573:44651-44670 Msa1443880:intron 40.0%
TTCATCTGTGATTCTGCCTT+TGG - tig0030573:44670-44689 Msa1443880:intergenic 40.0%
AAAGGCAGAATCACAGATGA+AGG + tig0030573:44669-44688 Msa1443880:CDS 40.0%
AGTGAGACTAAAAACACTGG+AGG + tig0030573:44779-44798 Msa1443880:CDS 40.0%
AGACTAAAAACACTGGAGGA+AGG + tig0030573:44783-44802 Msa1443880:CDS 40.0%
! TTGGACCACCATTTTCTGTT+GGG - tig0030573:44860-44879 Msa1443880:intergenic 40.0%
! TTTGGACCACCATTTTCTGT+TGG - tig0030573:44861-44880 Msa1443880:intergenic 40.0%
AAAACAACACACACTGTTGG+AGG + tig0030573:44949-44968 Msa1443880:CDS 40.0%
AAACAACACACACTGTTGGA+GGG + tig0030573:44950-44969 Msa1443880:CDS 40.0%
TGTTGGAGGGAATTCAAAAC+AGG + tig0030573:44963-44982 Msa1443880:CDS 40.0%
CCACTATCAGCAACTTTACT+TGG - tig0030573:45044-45063 Msa1443880:intergenic 40.0%
CCAAGTAAAGTTGCTGATAG+TGG + tig0030573:45041-45060 Msa1443880:CDS 40.0%
!! AGTTGCTGATAGTGGTGAAA+AGG + tig0030573:45049-45068 Msa1443880:CDS 40.0%
! CTTCACTTTCAGCATTGAAC+TGG - tig0030573:45112-45131 Msa1443880:intergenic 40.0%
ACACGTGTCAAACATTGACA+CGG - tig0030573:45383-45402 Msa1443880:intergenic 40.0%
GTCAGTATCAGAAACCAACA+CGG + tig0030573:45401-45420 Msa1443880:intron 40.0%
! GGGTTAGTGTCAATGTTGTT+TGG + tig0030573:45483-45502 Msa1443880:intron 40.0%
GAAACAAAGACTCCTCCAAA+AGG + tig0030573:45831-45850 Msa1443880:CDS 40.0%
! TATCAGCCACACTAACCTTT+TGG - tig0030573:45849-45868 Msa1443880:intergenic 40.0%
ATGAGAAAAGGACACTAGAG+AGG + tig0030573:43978-43997 Msa1443880:five_prime_UTR 40.0%
TGAGAAAAGGACACTAGAGA+GGG + tig0030573:43979-43998 Msa1443880:five_prime_UTR 40.0%
GCTAGCAAGGGTAAAAGTTT+CGG + tig0030573:44003-44022 Msa1443880:five_prime_UTR 40.0%
TCGGCCAATAGAATTGCAAA+TGG + tig0030573:44022-44041 Msa1443880:five_prime_UTR 40.0%
CAAATGGTGAAGCTAATGAG+TGG + tig0030573:44038-44057 Msa1443880:five_prime_UTR 40.0%
GTGGAAGGATGAAAATGACA+AGG + tig0030573:44057-44076 Msa1443880:five_prime_UTR 40.0%
TCTTCTAGCCATTACTTGAC+AGG - tig0030573:44088-44107 Msa1443880:intergenic 40.0%
AGTAATGGCTAGAAGAGAGA+AGG + tig0030573:44092-44111 Msa1443880:five_prime_UTR 40.0%
AGAAGAGAGAAGGATTATGC+AGG + tig0030573:44102-44121 Msa1443880:exon 40.0%
TTAAGGAGTCCAATCAAGAG+AGG + tig0030573:44322-44341 Msa1443880:intron 40.0%
ATATCAGAGAGAACAGCCAA+AGG + tig0030573:44651-44670 Msa1443880:intron 40.0%
TTCATCTGTGATTCTGCCTT+TGG - tig0030573:44670-44689 Msa1443880:intergenic 40.0%
AAAGGCAGAATCACAGATGA+AGG + tig0030573:44669-44688 Msa1443880:CDS 40.0%
AGTGAGACTAAAAACACTGG+AGG + tig0030573:44779-44798 Msa1443880:CDS 40.0%
AGACTAAAAACACTGGAGGA+AGG + tig0030573:44783-44802 Msa1443880:CDS 40.0%
! TTGGACCACCATTTTCTGTT+GGG - tig0030573:44860-44879 Msa1443880:intergenic 40.0%
! TTTGGACCACCATTTTCTGT+TGG - tig0030573:44861-44880 Msa1443880:intergenic 40.0%
AAAACAACACACACTGTTGG+AGG + tig0030573:44949-44968 Msa1443880:CDS 40.0%
AAACAACACACACTGTTGGA+GGG + tig0030573:44950-44969 Msa1443880:CDS 40.0%
TGTTGGAGGGAATTCAAAAC+AGG + tig0030573:44963-44982 Msa1443880:CDS 40.0%
CCACTATCAGCAACTTTACT+TGG - tig0030573:45044-45063 Msa1443880:intergenic 40.0%
CCAAGTAAAGTTGCTGATAG+TGG + tig0030573:45041-45060 Msa1443880:CDS 40.0%
!! AGTTGCTGATAGTGGTGAAA+AGG + tig0030573:45049-45068 Msa1443880:CDS 40.0%
! CTTCACTTTCAGCATTGAAC+TGG - tig0030573:45112-45131 Msa1443880:intergenic 40.0%
ACACGTGTCAAACATTGACA+CGG - tig0030573:45383-45402 Msa1443880:intergenic 40.0%
GTCAGTATCAGAAACCAACA+CGG + tig0030573:45401-45420 Msa1443880:intron 40.0%
! GGGTTAGTGTCAATGTTGTT+TGG + tig0030573:45483-45502 Msa1443880:intron 40.0%
GAAACAAAGACTCCTCCAAA+AGG + tig0030573:45831-45850 Msa1443880:CDS 40.0%
! TATCAGCCACACTAACCTTT+TGG - tig0030573:45849-45868 Msa1443880:intergenic 40.0%
TGGTGAAGCTAATGAGTGGA+AGG + tig0030573:44042-44061 Msa1443880:five_prime_UTR 45.0%
AGGTCATGCCTGTCAAGTAA+TGG + tig0030573:44077-44096 Msa1443880:five_prime_UTR 45.0%
AGTTCCCCAACAGAAAATGG+TGG + tig0030573:44852-44871 Msa1443880:CDS 45.0%
! TGGACCACCATTTTCTGTTG+GGG - tig0030573:44859-44878 Msa1443880:intergenic 45.0%
AGAAGCTCTTGGTTGTGATG+TGG - tig0030573:44892-44911 Msa1443880:intergenic 45.0%
GTATGATAGGCTGCAAAGAG+AGG + tig0030573:45220-45239 Msa1443880:CDS 45.0%
CACGTGTCAAACATTGACAC+GGG - tig0030573:45382-45401 Msa1443880:intergenic 45.0%
TTTCTTCCGCTCTACTTCCA+TGG - tig0030573:45738-45757 Msa1443880:intergenic 45.0%
AGATGCAGCTGCTAGAGAAA+AGG + tig0030573:45766-45785 Msa1443880:CDS 45.0%
ACTCCTCCAAAAGGTTAGTG+TGG + tig0030573:45840-45859 Msa1443880:CDS 45.0%
TGGTGAAGCTAATGAGTGGA+AGG + tig0030573:44042-44061 Msa1443880:five_prime_UTR 45.0%
AGGTCATGCCTGTCAAGTAA+TGG + tig0030573:44077-44096 Msa1443880:five_prime_UTR 45.0%
AGTTCCCCAACAGAAAATGG+TGG + tig0030573:44852-44871 Msa1443880:CDS 45.0%
! TGGACCACCATTTTCTGTTG+GGG - tig0030573:44859-44878 Msa1443880:intergenic 45.0%
AGAAGCTCTTGGTTGTGATG+TGG - tig0030573:44892-44911 Msa1443880:intergenic 45.0%
GTATGATAGGCTGCAAAGAG+AGG + tig0030573:45220-45239 Msa1443880:CDS 45.0%
CACGTGTCAAACATTGACAC+GGG - tig0030573:45382-45401 Msa1443880:intergenic 45.0%
TTTCTTCCGCTCTACTTCCA+TGG - tig0030573:45738-45757 Msa1443880:intergenic 45.0%
AGATGCAGCTGCTAGAGAAA+AGG + tig0030573:45766-45785 Msa1443880:CDS 45.0%
ACTCCTCCAAAAGGTTAGTG+TGG + tig0030573:45840-45859 Msa1443880:CDS 45.0%
ACTAGAGAGGGAGCTAGCAA+GGG + tig0030573:43991-44010 Msa1443880:five_prime_UTR 50.0%
GTTGGACAGCCTCTCTTGAT+TGG - tig0030573:44334-44353 Msa1443880:intergenic 50.0%
CAACCAAGAGCTTCTGCGAT+TGG + tig0030573:44897-44916 Msa1443880:CDS 50.0%
CTTCCAATCGCAGAAGCTCT+TGG - tig0030573:44903-44922 Msa1443880:intergenic 50.0%
TCGTCACTGCACGAGTCATT+TGG - tig0030573:45182-45201 Msa1443880:intergenic 50.0%
TGACTCGTGCAGTGACGATT+TGG + tig0030573:45184-45203 Msa1443880:CDS 50.0%
!! TGCAGTGACGATTTGGTGTC+AGG + tig0030573:45191-45210 Msa1443880:CDS 50.0%
TGACACTAACCCGAAGACAC+CGG - tig0030573:45475-45494 Msa1443880:intergenic 50.0%
! TGATGCACTGACCAAGTCCA+TGG + tig0030573:45718-45737 Msa1443880:intron 50.0%
CCAAGTCCATGGAAGTAGAG+CGG + tig0030573:45729-45748 Msa1443880:intron 50.0%
TGCAGCTGCTAGAGAAAAGG+AGG + tig0030573:45769-45788 Msa1443880:CDS 50.0%
! CAGCCACACTAACCTTTTGG+AGG - tig0030573:45846-45865 Msa1443880:intergenic 50.0%
ACTAGAGAGGGAGCTAGCAA+GGG + tig0030573:43991-44010 Msa1443880:five_prime_UTR 50.0%
GTTGGACAGCCTCTCTTGAT+TGG - tig0030573:44334-44353 Msa1443880:intergenic 50.0%
CAACCAAGAGCTTCTGCGAT+TGG + tig0030573:44897-44916 Msa1443880:CDS 50.0%
CTTCCAATCGCAGAAGCTCT+TGG - tig0030573:44903-44922 Msa1443880:intergenic 50.0%
TCGTCACTGCACGAGTCATT+TGG - tig0030573:45182-45201 Msa1443880:intergenic 50.0%
TGACTCGTGCAGTGACGATT+TGG + tig0030573:45184-45203 Msa1443880:CDS 50.0%
!! TGCAGTGACGATTTGGTGTC+AGG + tig0030573:45191-45210 Msa1443880:CDS 50.0%
TGACACTAACCCGAAGACAC+CGG - tig0030573:45475-45494 Msa1443880:intergenic 50.0%
! TGATGCACTGACCAAGTCCA+TGG + tig0030573:45718-45737 Msa1443880:intron 50.0%
CCAAGTCCATGGAAGTAGAG+CGG + tig0030573:45729-45748 Msa1443880:intron 50.0%
TGCAGCTGCTAGAGAAAAGG+AGG + tig0030573:45769-45788 Msa1443880:CDS 50.0%
! CAGCCACACTAACCTTTTGG+AGG - tig0030573:45846-45865 Msa1443880:intergenic 50.0%
CACTAGAGAGGGAGCTAGCA+AGG + tig0030573:43990-44009 Msa1443880:five_prime_UTR 55.0%
ACCAACACGGTCCTGACACA+TGG + tig0030573:45414-45433 Msa1443880:intron 55.0%
ACCATGTGTCAGGACCGTGT+TGG - tig0030573:45418-45437 Msa1443880:intergenic 55.0%
CCGCTCTACTTCCATGGACT+TGG - tig0030573:45732-45751 Msa1443880:intergenic 55.0%
CACTAGAGAGGGAGCTAGCA+AGG + tig0030573:43990-44009 Msa1443880:five_prime_UTR 55.0%
ACCAACACGGTCCTGACACA+TGG + tig0030573:45414-45433 Msa1443880:intron 55.0%
ACCATGTGTCAGGACCGTGT+TGG - tig0030573:45418-45437 Msa1443880:intergenic 55.0%
CCGCTCTACTTCCATGGACT+TGG - tig0030573:45732-45751 Msa1443880:intergenic 55.0%
Chromosome Type Strat End Strand Name
tig0030573 gene 43865 45910 43865 ID=Msa1443880;Name=Msa1443880
tig0030573 mRNA 43865 45910 43865 ID=Msa1443880-mRNA-1;Parent=Msa1443880;Name=Msa1443880-mRNA-1;_AED=0.11;_eAED=0.11;_QI=253|0.25|0.2|1|0.25|0.4|5|0|219
tig0030573 exon 43865 44123 43865 ID=Msa1443880-mRNA-1:exon:14163;Parent=Msa1443880-mRNA-1
tig0030573 exon 44658 44690 44658 ID=Msa1443880-mRNA-1:exon:14164;Parent=Msa1443880-mRNA-1
tig0030573 exon 44768 44988 44768 ID=Msa1443880-mRNA-1:exon:14165;Parent=Msa1443880-mRNA-1
tig0030573 exon 45019 45241 45019 ID=Msa1443880-mRNA-1:exon:14166;Parent=Msa1443880-mRNA-1
tig0030573 exon 45734 45910 45734 ID=Msa1443880-mRNA-1:exon:14167;Parent=Msa1443880-mRNA-1
tig0030573 five_prime_UTR 43865 44117 43865 ID=Msa1443880-mRNA-1:five_prime_utr;Parent=Msa1443880-mRNA-1
tig0030573 CDS 44118 44123 44118 ID=Msa1443880-mRNA-1:cds;Parent=Msa1443880-mRNA-1
tig0030573 CDS 44658 44690 44658 ID=Msa1443880-mRNA-1:cds;Parent=Msa1443880-mRNA-1
tig0030573 CDS 44768 44988 44768 ID=Msa1443880-mRNA-1:cds;Parent=Msa1443880-mRNA-1
tig0030573 CDS 45019 45241 45019 ID=Msa1443880-mRNA-1:cds;Parent=Msa1443880-mRNA-1
tig0030573 CDS 45734 45910 45734 ID=Msa1443880-mRNA-1:cds;Parent=Msa1443880-mRNA-1
Gene Sequence

>Msa1443880

ATGCAGAGAGAACAGCCAAAGGCAGAATCACAGATGAAGGAAAAGCTAAAAGTGAGACTAAAAACACTGGAGGAAGGTTTAAAACATTCTTCAAGTTCAAAAGCAGAAAAGTCTAACATCATAAGTTCCCCAACAGAAAATGGTGGTCCAAAAAAGAGATCCACATCACAACCAAGAGCTTCTGCGATTGGAAGCCCTTTGTTTCAACAGACAAATATAAAAAACAACACACACTGTTGGAGGGAATTCAAAACAGGAAAAAAACATGTTGAAGAAAAGGATCCAAGTAAAGTTGCTGATAGTGGTGAAAAGGAAAATGAGATACAGGTGGACACAGACATGAATTTAAACCAGTTCAATGCTGAAAGTGAAGAAATCAAAACAATTGTTGATGTGAATGAAGACTTCCAAAGCAAGAAACCAAATGACTCGTGCAGTGACGATTTGGTGTCAGGTTTTTTGTATGATAGGCTGCAAAGAGAGTCCATGGAAGTAGAGCGGAAGAAAATGAAAAGAGATGCAGCTGCTAGAGAAAAGGAGGTTTCAGCAATAATAAGTTCAGATGATAACAGGAAAAACAGAAACAAAGACTCCTCCAAAAGGTTAGTGTGGCTGATACATGCTAACAAGTTTGTTCTGTTTGTCTTAACTATAGCCTAA
Protein sequence

>Msa1443880

MQREQPKAESQMKEKLKVRLKTLEEGLKHSSSSKAEKSNIISSPTENGGPKKRSTSQPRASAIGSPLFQQTNIKNNTHCWREFKTGKKHVEEKDPSKVADSGEKENEIQVDTDMNLNQFNAESEEIKTIVDVNEDFQSKKPNDSCSDDLVSGFLYDRLQRESMEVERKKMKRDAAAREKEVSAIISSDDNRKNRNKDSSKRLVWLIHANKFVLFVLTIA*