AlfalfaGEDB | Msa1224340

M. sativa cultivar zhongmu-4 / Msa1224340

KEGG: -
GO: GO:0003002 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005874 GO:0006996 GO:0007010 GO:0007275 GO:0007389 GO:0008017 GO:0008092 GO:0008150 GO:0009832 GO:0009987 GO:0010051 GO:0015630 GO:0015631 GO:0016043 GO:0032501 GO:0032502 GO:0042546 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0048856 GO:0071554 GO:0071669 GO:0071840 GO:0099080 GO:0099081 GO:0099512 GO:0099513
PFAM: PF07058.14

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1224340	KEH32682.1	63.823	293	19	5	1	206	293	585	6.98e-107	329

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1224340	sp\|Q8GYX3\|MP705_ARATH	37.168	226	70	7	1	168	273	484	4.80e-26	107

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1224340	A0A072USN7	63.823	293	19	5	1	206	293	585	3.33e-107	329

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0074700	Msa1224340	0.808317	3.286402e-50	-8.615850e-47
Msa0090100	Msa1224340	0.803937	2.746954e-49	-8.615850e-47
Msa0095560	Msa1224340	0.800970	1.122391e-48	-8.615850e-47
Msa0122590	Msa1224340	0.807391	5.173201e-50	-8.615850e-47
Msa0157310	Msa1224340	0.810425	1.159904e-50	-8.615850e-47
Msa0166870	Msa1224340	0.827022	1.967548e-54	-8.615850e-47
Msa0188830	Msa1224340	0.805053	1.607523e-49	-8.615850e-47
Msa0201070	Msa1224340	0.807353	5.269966e-50	-8.615850e-47
Msa0220580	Msa1224340	0.812180	4.826162e-51	-8.615850e-47
Msa0223010	Msa1224340	0.801733	7.834145e-49	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1224340	MtrunA17_Chr4g0076251	61.511	278	20	5	1	191	293	570	5.91e-97	293
Msa1224340	MtrunA17_Chr2g0290411	39.789	284	64	9	1	191	280	549	3.23e-39	141
Msa1224340	MtrunA17_Chr8g0344311	34.400	250	84	11	1	172	320	567	6.75e-15	72.8
Msa1224340	MtrunA17_Chr8g0391771	32.411	253	87	10	1	172	324	573	3.28e-13	67.8
Msa1224340	MtrunA17_Chr1g0161371	30.279	251	95	8	1	172	319	568	3.55e-11	61.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1224340	AT4G17220.1	37.168	226	70	7	1	168	273	484	4.88e-27	107
Msa1224340	AT1G24764.2	31.907	257	85	10	1	172	188	439	9.57e-14	69.7
Msa1224340	AT1G24764.3	31.907	257	85	10	1	172	400	651	1.29e-13	69.3
Msa1224340	AT1G24764.1	31.907	257	85	10	1	172	341	592	1.34e-13	69.3
Msa1224340	AT2G01750.2	29.496	278	94	10	1	188	340	605	9.03e-13	67.0
Msa1224340	AT2G01750.1	29.496	278	94	10	1	188	340	605	9.28e-13	66.6
Msa1224340	AT1G68060.1	28.571	266	108	8	1	187	333	595	3.29e-12	65.1

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Find 44 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGAAAATGAGATACAGG+TGG	0.667770	8_1:+89148670	Msa1224340:CDS
ACTAGAGAGGGAGCTAGCAA+GGG	0.615439	8_1:+89147595	Msa1224340:five_prime_UTR
ACTCCTCCAAAAGGTTAGTG+TGG	0.610939	8_1:+89149443	Msa1224340:CDS
AGAAGAGAGAAGGATTATGC+AGG	0.567503	8_1:+89147706	Msa1224340:CDS
AGAAGCTCTTGGTTGTGATG+TGG	0.668929	8_1:-89148492	None:intergenic
AGATGCAGCTGCTAGAGAAA+AGG	0.532223	8_1:+89149369	Msa1224340:CDS
AGCAATGGCTAGAAGAGAGA+AGG	0.577331	8_1:+89147696	Msa1224340:CDS
AGGTCATGCCTGTCAAGCAA+TGG	0.604618	8_1:+89147681	Msa1224340:exon
AGTCATTTGGTTTCTTGCTT+TGG	0.339262	8_1:-89148769	None:intergenic
AGTTCCCCAACAGAAAATGG+TGG	0.700541	8_1:+89148455	Msa1224340:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TTTGTTTGGTTGATCTTATG+TGG	+	chr8_1:89147528-89147547	Msa1224340:five_prime_UTR	30.0%
TTCACCATTTGCAATTCTAT+TGG	-	chr8_1:89147633-89147652	None:intergenic	30.0%
CAGGATCATATAAACTTCAA+AGG	-	chr8_1:89147764-89147783	None:intergenic	30.0%
TTGATTGGACTCCTTAAATA+TGG	-	chr8_1:89147923-89147942	None:intergenic	30.0%
TATTATAAGCGCATTAGTGT+TGG	-	chr8_1:89147956-89147975	None:intergenic	30.0%
CTACATTTGCAAAGACATAA+TGG	-	chr8_1:89148202-89148221	None:intergenic	30.0%
AAAAGTGAGACTAAAAACAC+TGG	+	chr8_1:89148379-89148398	Msa1224340:CDS	30.0%
TAAAAAACAACACACACTGT+TGG	+	chr8_1:89148549-89148568	Msa1224340:intron	30.0%
CAGAAAACATGTTGAAGAAA+AGG	+	chr8_1:89148619-89148638	Msa1224340:intron	30.0%
TGAAAAGGAAAATGAGATAC+AGG	+	chr8_1:89148667-89148686	Msa1224340:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr8_1	gene	89147469	89149513	89147469	ID=Msa1224340;Name=Msa1224340
chr8_1	mRNA	89147469	89149513	89147469	ID=Msa1224340-mRNA-1;Parent=Msa1224340;Name=Msa1224340-mRNA-1;_AED=0.12;_eAED=0.12;_QI=217\|0\|0.25\|1\|1\|1\|4\|0\|207
chr8_1	exon	89147469	89147727	89147469	ID=Msa1224340-mRNA-1:exon:32377;Parent=Msa1224340-mRNA-1
chr8_1	exon	89148371	89148531	89148371	ID=Msa1224340-mRNA-1:exon:32378;Parent=Msa1224340-mRNA-1
chr8_1	exon	89148622	89148844	89148622	ID=Msa1224340-mRNA-1:exon:32379;Parent=Msa1224340-mRNA-1
chr8_1	exon	89149316	89149513	89149316	ID=Msa1224340-mRNA-1:exon:32380;Parent=Msa1224340-mRNA-1
chr8_1	five_prime_UTR	89147469	89147685	89147469	ID=Msa1224340-mRNA-1:five_prime_utr;Parent=Msa1224340-mRNA-1
chr8_1	CDS	89147686	89147727	89147686	ID=Msa1224340-mRNA-1:cds;Parent=Msa1224340-mRNA-1
chr8_1	CDS	89148371	89148531	89148371	ID=Msa1224340-mRNA-1:cds;Parent=Msa1224340-mRNA-1
chr8_1	CDS	89148622	89148844	89148622	ID=Msa1224340-mRNA-1:cds;Parent=Msa1224340-mRNA-1
chr8_1	CDS	89149316	89149513	89149316	ID=Msa1224340-mRNA-1:cds;Parent=Msa1224340-mRNA-1

Gene Sequence

>Msa1224340

ATGCCTGTCAAGCAATGGCTAGAAGAGAGAAGGATTATGCAGGAAAAGCTAAAAGTGAGACTAAAAACACTGGAGGAAGGTTTAAAACATTCTTCAAGTTCAAAAGCAGAAAAGTCTAACATCATAAGTTCCCCAACAGAAAATGGTGGTCCAAAAAAGAGATCCACATCACAACCAAGAGCTTCTGCGATTGGAAGCCCTTTAAAACATGTTGAAGAAAAGGATCCAAGTAAAGTTGCTGATAGTGGTGAAAAGGAAAATGAGATACAGGTGGACACAGACATGAATTTAAACCAGTTCAATGCTGAAAGTGAAGAAATCAAAACAATTGTTGATGTGAATGAAGACTTCCAAAGCAAGAAACCAAATGACTCGTGCAGTGACGATTTGGTGTCAGGTTTTTTGTATGATAGGCTGCAAAGAGAGAAAGTTGATGCACTGACCAAGTCCATGGAAGTAGAGCGGAAGAAAATGAAAAGAGATGCAGCTGCTAGAGAAAAGGAGGTTTCAGCAATAATAAGTTCAGATGATAACAGGAAAAACAGAAACAAAGACTCCTCCAAAAGGTTAGTGTGGCTGATACATGCTAACAAGTTTGTTCTGTTTGTCTTAACTATAGCCTAA

Protein sequence

>Msa1224340

MPVKQWLEERRIMQEKLKVRLKTLEEGLKHSSSSKAEKSNIISSPTENGGPKKRSTSQPRASAIGSPLKHVEEKDPSKVADSGEKENEIQVDTDMNLNQFNAESEEIKTIVDVNEDFQSKKPNDSCSDDLVSGFLYDRLQREKVDALTKSMEVERKKMKRDAAAREKEVSAIISSDDNRKNRNKDSSKRLVWLIHANKFVLFVLTIA*