Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1422340 | sp|P24076|BGIA_MOMCH | 64.706 | 68 | 24 | 0 | 3 | 70 | 1 | 68 | 5.47e-25 | 90.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1422340 | G7KCZ8 | 94.286 | 70 | 4 | 0 | 1 | 70 | 1 | 70 | 6.66e-41 | 137 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0052780 | Msa1422340 | 0.802325 | 5.920228e-49 | -8.615850e-47 |
| Msa0077370 | Msa1422340 | 0.837298 | 5.646749e-57 | -8.615850e-47 |
| Msa0097570 | Msa1422340 | 0.804832 | 1.787422e-49 | -8.615850e-47 |
| Msa0611060 | Msa1422340 | 0.811255 | 7.670844e-51 | -8.615850e-47 |
| Msa0708600 | Msa1422340 | 0.813658 | 2.290180e-51 | -8.615850e-47 |
| Msa0744640 | Msa1422340 | 0.828914 | 6.897907e-55 | -8.615850e-47 |
| Msa0841730 | Msa1422340 | 0.829357 | 5.386655e-55 | -8.615850e-47 |
| Msa0125320 | Msa1422340 | 0.801621 | 8.259488e-49 | -8.615850e-47 |
| Msa0528990 | Msa1422340 | 0.802807 | 4.708999e-49 | -8.615850e-47 |
| Msa0529000 | Msa1422340 | 0.820317 | 7.311358e-53 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1422340 | MtrunA17_Chr5g0442291 | 94.286 | 70 | 4 | 0 | 1 | 70 | 1 | 70 | 1.28e-44 | 137 |
| Msa1422340 | MtrunA17_Chr1g0187221 | 89.706 | 68 | 7 | 0 | 3 | 70 | 5 | 72 | 8.10e-39 | 122 |
| Msa1422340 | MtrunA17_Chr1g0187161 | 84.286 | 70 | 11 | 0 | 1 | 70 | 1 | 70 | 6.31e-38 | 120 |
| Msa1422340 | MtrunA17_Chr1g0187201 | 82.353 | 68 | 12 | 0 | 3 | 70 | 4 | 71 | 1.05e-33 | 110 |
| Msa1422340 | MtrunA17_Chr1g0184411 | 60.000 | 70 | 28 | 0 | 1 | 70 | 1 | 70 | 6.46e-24 | 85.1 |
| Msa1422340 | MtrunA17_Chr5g0419531 | 62.121 | 66 | 25 | 0 | 5 | 70 | 4 | 69 | 7.84e-24 | 85.1 |
| Msa1422340 | MtrunA17_Chr1g0184401 | 60.606 | 66 | 26 | 0 | 5 | 70 | 8 | 73 | 6.70e-23 | 82.8 |
| Msa1422340 | MtrunA17_Chr3g0085011 | 50.794 | 63 | 31 | 0 | 8 | 70 | 36 | 98 | 1.64e-14 | 62.4 |
| Msa1422340 | MtrunA17_Chr3g0084991 | 42.857 | 63 | 36 | 0 | 8 | 70 | 32 | 94 | 2.70e-11 | 53.9 |
| Msa1422340 | MtrunA17_Chr3g0084931 | 47.619 | 63 | 33 | 0 | 8 | 70 | 19 | 81 | 2.96e-11 | 53.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1422340 | AT2G38870.1 | 60.000 | 70 | 28 | 0 | 1 | 70 | 1 | 70 | 5.56e-24 | 85.9 |
| Msa1422340 | AT5G43580.1 | 54.688 | 64 | 29 | 0 | 7 | 70 | 36 | 99 | 6.77e-19 | 73.6 |
| Msa1422340 | AT2G38900.1 | 48.438 | 64 | 33 | 0 | 7 | 70 | 10 | 73 | 1.33e-16 | 67.0 |
| Msa1422340 | AT2G38900.2 | 48.438 | 64 | 33 | 0 | 7 | 70 | 25 | 88 | 1.99e-16 | 67.0 |
| Msa1422340 | AT3G46860.1 | 47.619 | 63 | 33 | 0 | 8 | 70 | 23 | 85 | 1.56e-13 | 59.7 |
| Msa1422340 | AT5G43570.1 | 45.946 | 74 | 34 | 1 | 1 | 68 | 1 | 74 | 4.67e-13 | 58.9 |
| Msa1422340 | AT5G43570.2 | 49.180 | 61 | 31 | 0 | 8 | 68 | 24 | 84 | 4.60e-12 | 56.6 |
| Msa1422340 | AT5G45277.1 | 47.170 | 53 | 28 | 0 | 8 | 60 | 2 | 54 | 7.88e-11 | 52.4 |
Find 35 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCTCCAAGTTATAAATTTA+AGG | 0.116946 | tig0025360:+49892 | Msa1422340:three_prime_UTR |
| GTCTATCAGGTTCCAGTAAT+TGG | 0.190800 | tig0025360:+49830 | Msa1422340:CDS |
| CTGTGACAGGGTTAGGGTTT+GGG | 0.262714 | tig0025360:+49790 | Msa1422340:CDS |
| ACTTCCGCTGTGACAGGGTT+AGG | 0.276358 | tig0025360:+49783 | Msa1422340:CDS |
| GATACTTTCTATCCAATTAC+TGG | 0.287675 | tig0025360:-49842 | None:intergenic |
| TAAAGATGGAATTGTCTATC+AGG | 0.346883 | tig0025360:+49817 | Msa1422340:CDS |
| GCTGTGACAGGGTTAGGGTT+TGG | 0.358066 | tig0025360:+49789 | Msa1422340:CDS |
| ATAAATTTAAGGTTTCAATA+AGG | 0.380429 | tig0025360:+49903 | Msa1422340:three_prime_UTR |
| ATGCAGAACATGTATACAAT+TGG | 0.403164 | tig0025360:-49939 | None:intergenic |
| AGGGTTTGGGTTGATAAAGA+TGG | 0.412719 | tig0025360:+49803 | Msa1422340:CDS |
| GAAACCTTAAATTTATAACT+TGG | 0.420821 | tig0025360:-49896 | None:intergenic |
| CCGCCGACTTCCGCTGTGAC+AGG | 0.421191 | tig0025360:+49777 | Msa1422340:CDS |
| CTTCCGCTGTGACAGGGTTA+GGG | 0.422816 | tig0025360:+49784 | Msa1422340:CDS |
| TACATATTGAAGGATCAAAA+AGG | 0.425186 | tig0025360:+49515 | Msa1422340:five_prime_UTR |
| CCTTCCACTCCAACCAACTC+TGG | 0.454716 | tig0025360:-49677 | None:intergenic |
| GAAATAGTCATACATATTGA+AGG | 0.490665 | tig0025360:+49505 | Msa1422340:five_prime_UTR |
| AGATCCTTCTGGCACAATGA+TGG | 0.502286 | tig0025360:-49747 | None:intergenic |
| CCAGAGTTGGTTGGAGTGGA+AGG | 0.515293 | tig0025360:+49677 | Msa1422340:CDS |
| GTTGGTTGGAGTGGAAGGAA+AGG | 0.527079 | tig0025360:+49682 | Msa1422340:CDS |
| GCGGTGACAAAAGATCCTTC+TGG | 0.550057 | tig0025360:-49758 | None:intergenic |
| TATGGTCAGGTAAGAGCTCA+TGG | 0.552544 | tig0025360:+49654 | Msa1422340:intron |
| CGCCGACTTCCGCTGTGACA+GGG | 0.553611 | tig0025360:+49778 | Msa1422340:CDS |
| ATGGCCAGAGTTGGTTGGAG+TGG | 0.564909 | tig0025360:+49673 | Msa1422340:CDS |
| AATTGAGAGGGAGAATCCAT+TGG | 0.570404 | tig0025360:+49718 | Msa1422340:CDS |
| AACCCTGTCACAGCGGAAGT+CGG | 0.575336 | tig0025360:-49780 | None:intergenic |
| AGCTCATGGCCAGAGTTGGT+TGG | 0.578782 | tig0025360:+49668 | Msa1422340:CDS |
| TAATTGGATAGAAAGTATCA+TGG | 0.579980 | tig0025360:+49846 | Msa1422340:exon |
| TAAGAGCTCATGGCCAGAGT+TGG | 0.585826 | tig0025360:+49664 | Msa1422340:CDS |
| AGCAGAAGCTACAATTGAGA+GGG | 0.593359 | tig0025360:+49706 | Msa1422340:CDS |
| TAGCAGAAGCTACAATTGAG+AGG | 0.609404 | tig0025360:+49705 | Msa1422340:CDS |
| AATGCCATCATTGTGCCAGA+AGG | 0.618440 | tig0025360:+49743 | Msa1422340:CDS |
| ACAATGATGGCATTCACCAA+TGG | 0.635361 | tig0025360:-49734 | None:intergenic |
| CCTGTCACAGCGGAAGTCGG+CGG | 0.661655 | tig0025360:-49777 | None:intergenic |
| AAAATGTCTGATGAATGCAA+AGG | 0.675933 | tig0025360:+49545 | Msa1422340:exon |
| AAACCCTAACCCTGTCACAG+CGG | 0.735906 | tig0025360:-49787 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAATTTTAATTATAATTTGA+TGG | + | tig0025360:49613-49632 | Msa1422340:intron | 10.0% |
| !! | TTGAATTAATCAATATTGTA+TGG | + | tig0025360:49636-49655 | Msa1422340:intron | 15.0% |
| !!! | ATAAATTTAAGGTTTCAATA+AGG | + | tig0025360:49903-49922 | Msa1422340:three_prime_UTR | 15.0% |
| !! | TGTTTAAATTAAATGGTTTG+TGG | + | tig0025360:49591-49610 | Msa1422340:intron | 20.0% |
| !! | GAAACCTTAAATTTATAACT+TGG | - | tig0025360:49899-49918 | None:intergenic | 20.0% |
| !!! | TCATTTTCTTTAATTTGCAA+AGG | + | tig0025360:49998-50017 | Msa1422340:three_prime_UTR | 20.0% |
| ! | GAAATAGTCATACATATTGA+AGG | + | tig0025360:49505-49524 | Msa1422340:five_prime_UTR | 25.0% |
| ! | TACATATTGAAGGATCAAAA+AGG | + | tig0025360:49515-49534 | Msa1422340:five_prime_UTR | 25.0% |
| ! | TTAATCAATATTGTATGGTC+AGG | + | tig0025360:49641-49660 | Msa1422340:intron | 25.0% |
| !!! | TAATTGGATAGAAAGTATCA+TGG | + | tig0025360:49846-49865 | Msa1422340:exon | 25.0% |
| ! | TGCTCCAAGTTATAAATTTA+AGG | + | tig0025360:49892-49911 | Msa1422340:three_prime_UTR | 25.0% |
| ACACAAGAATTGCAAAAAGA+AGG | + | tig0025360:49471-49490 | Msa1422340:five_prime_UTR | 30.0% | |
| AAAATGTCTGATGAATGCAA+AGG | + | tig0025360:49545-49564 | Msa1422340:exon | 30.0% | |
| TAAAGATGGAATTGTCTATC+AGG | + | tig0025360:49817-49836 | Msa1422340:CDS | 30.0% | |
| GATACTTTCTATCCAATTAC+TGG | - | tig0025360:49845-49864 | None:intergenic | 30.0% | |
| ATGCAGAACATGTATACAAT+TGG | - | tig0025360:49942-49961 | None:intergenic | 30.0% | |
| GTCTGATGAATGCAAAGGTA+AGG | + | tig0025360:49550-49569 | Msa1422340:intron | 40.0% | |
| TAGCAGAAGCTACAATTGAG+AGG | + | tig0025360:49705-49724 | Msa1422340:CDS | 40.0% | |
| AGCAGAAGCTACAATTGAGA+GGG | + | tig0025360:49706-49725 | Msa1422340:CDS | 40.0% | |
| AATTGAGAGGGAGAATCCAT+TGG | + | tig0025360:49718-49737 | Msa1422340:CDS | 40.0% | |
| ACAATGATGGCATTCACCAA+TGG | - | tig0025360:49737-49756 | None:intergenic | 40.0% | |
| !! | AGGGTTTGGGTTGATAAAGA+TGG | + | tig0025360:49803-49822 | Msa1422340:CDS | 40.0% |
| GTCTATCAGGTTCCAGTAAT+TGG | + | tig0025360:49830-49849 | Msa1422340:CDS | 40.0% | |
| TATGGTCAGGTAAGAGCTCA+TGG | + | tig0025360:49654-49673 | Msa1422340:intron | 45.0% | |
| AATGCCATCATTGTGCCAGA+AGG | + | tig0025360:49743-49762 | Msa1422340:CDS | 45.0% | |
| AGATCCTTCTGGCACAATGA+TGG | - | tig0025360:49750-49769 | None:intergenic | 45.0% | |
| !! | TATAATATGTTTAAATTAAA+TGG | + | tig0025360:49584-49603 | Msa1422340:intron | 5.0% |
| TAAGAGCTCATGGCCAGAGT+TGG | + | tig0025360:49664-49683 | Msa1422340:CDS | 50.0% | |
| GTTGGTTGGAGTGGAAGGAA+AGG | + | tig0025360:49682-49701 | Msa1422340:CDS | 50.0% | |
| GCGGTGACAAAAGATCCTTC+TGG | - | tig0025360:49761-49780 | None:intergenic | 50.0% | |
| AAACCCTAACCCTGTCACAG+CGG | - | tig0025360:49790-49809 | None:intergenic | 50.0% | |
| CTGTGACAGGGTTAGGGTTT+GGG | + | tig0025360:49790-49809 | Msa1422340:CDS | 50.0% | |
| ! | AGCTCATGGCCAGAGTTGGT+TGG | + | tig0025360:49668-49687 | Msa1422340:CDS | 55.0% |
| ! | ATGGCCAGAGTTGGTTGGAG+TGG | + | tig0025360:49673-49692 | Msa1422340:CDS | 55.0% |
| CCTTCCACTCCAACCAACTC+TGG | - | tig0025360:49680-49699 | None:intergenic | 55.0% | |
| ! | CCAGAGTTGGTTGGAGTGGA+AGG | + | tig0025360:49677-49696 | Msa1422340:CDS | 55.0% |
| AACCCTGTCACAGCGGAAGT+CGG | - | tig0025360:49783-49802 | None:intergenic | 55.0% | |
| ACTTCCGCTGTGACAGGGTT+AGG | + | tig0025360:49783-49802 | Msa1422340:CDS | 55.0% | |
| CTTCCGCTGTGACAGGGTTA+GGG | + | tig0025360:49784-49803 | Msa1422340:CDS | 55.0% | |
| GCTGTGACAGGGTTAGGGTT+TGG | + | tig0025360:49789-49808 | Msa1422340:CDS | 55.0% | |
| CCTGTCACAGCGGAAGTCGG+CGG | - | tig0025360:49780-49799 | None:intergenic | 65.0% | |
| !! | CGCCGACTTCCGCTGTGACA+GGG | + | tig0025360:49778-49797 | Msa1422340:CDS | 65.0% |
| !! | CCGCCGACTTCCGCTGTGAC+AGG | + | tig0025360:49777-49796 | Msa1422340:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0025360 | gene | 49446 | 50053 | 49446 | ID=Msa1422340;Name=Msa1422340 |
| tig0025360 | mRNA | 49446 | 50053 | 49446 | ID=Msa1422340-mRNA-1;Parent=Msa1422340;Name=Msa1422340-mRNA-1;_AED=0.02;_eAED=0.02;_QI=102|1|1|1|1|1|2|197|70 |
| tig0025360 | exon | 49446 | 49566 | 49446 | ID=Msa1422340-mRNA-1:exon:2483;Parent=Msa1422340-mRNA-1 |
| tig0025360 | exon | 49663 | 50053 | 49663 | ID=Msa1422340-mRNA-1:exon:2484;Parent=Msa1422340-mRNA-1 |
| tig0025360 | five_prime_UTR | 49446 | 49547 | 49446 | ID=Msa1422340-mRNA-1:five_prime_utr;Parent=Msa1422340-mRNA-1 |
| tig0025360 | CDS | 49548 | 49566 | 49548 | ID=Msa1422340-mRNA-1:cds;Parent=Msa1422340-mRNA-1 |
| tig0025360 | CDS | 49663 | 49856 | 49663 | ID=Msa1422340-mRNA-1:cds;Parent=Msa1422340-mRNA-1 |
| tig0025360 | three_prime_UTR | 49857 | 50053 | 49857 | ID=Msa1422340-mRNA-1:three_prime_utr;Parent=Msa1422340-mRNA-1 |
| Gene Sequence |
| Protein sequence |
