AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0529000


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0529000 MtrunA17_Chr1g0187161 84.286 70 11 0 1 70 1 70 4.87e-37 118
Msa0529000 MtrunA17_Chr1g0187201 88.235 68 8 0 3 70 4 71 8.29e-37 117
Msa0529000 MtrunA17_Chr1g0187221 81.429 70 13 0 1 70 3 72 1.67e-35 114
Msa0529000 MtrunA17_Chr5g0442291 82.857 70 12 0 1 70 1 70 3.98e-35 113
Msa0529000 MtrunA17_Chr1g0184411 54.286 70 32 0 1 70 1 70 1.24e-20 77.0
Msa0529000 MtrunA17_Chr1g0184401 53.030 66 31 0 5 70 8 73 6.82e-18 70.1
Msa0529000 MtrunA17_Chr5g0419531 53.030 66 31 0 5 70 4 69 1.72e-17 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0529000 AT2G38870.1 52.857 70 33 0 1 70 1 70 1.55e-20 77.0
Msa0529000 AT5G43580.1 48.611 72 35 1 1 70 28 99 8.35e-18 70.9
Msa0529000 AT3G46860.1 42.857 70 37 1 4 70 16 85 6.68e-13 58.2
Msa0529000 AT2G38900.2 45.312 64 35 0 7 70 25 88 1.56e-12 57.4
Msa0529000 AT2G38900.1 45.312 64 35 0 7 70 10 73 1.94e-12 56.6
Msa0529000 AT5G43570.1 46.575 73 33 1 1 67 1 73 1.43e-11 55.1

Find 33 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGCAATTCTTCTTGTGTT+TGG 0.271522 4_1:+2003654 None:intergenic
CAACGACAGGGTTTGGGTTT+GGG 0.289389 4_1:-2003314 Msa0529000:CDS
GCAACGACAGGGTTTGGGTT+TGG 0.327218 4_1:-2003315 Msa0529000:CDS
GACACTTTCTATCCAATTGT+TGG 0.347121 4_1:+2003262 None:intergenic
ATAAATTTAATGTTTCAATA+AGG 0.348781 4_1:-2003207 Msa0529000:three_prime_UTR
ACTTACGCAACGACAGGGTT+TGG 0.351229 4_1:-2003321 Msa0529000:CDS
CTTACGCAACGACAGGGTTT+GGG 0.360903 4_1:-2003320 Msa0529000:CDS
GTCACTCAGGTTCCAACAAT+TGG 0.363160 4_1:-2003274 Msa0529000:CDS
TATAATAAGTAGTATACAAT+TGG 0.368616 4_1:+2003171 None:intergenic
TGGGTTTGGGTTAATAAAGA+TGG 0.418377 4_1:-2003301 Msa0529000:CDS
TACATATTGAAGGATCAAAA+AGG 0.425186 4_1:-2003602 Msa0529000:five_prime_UTR
CCTTCCACTCCAACCAACTC+TGG 0.454716 4_1:+2003427 None:intergenic
AATGCCATCATTGTGCTAGA+AGG 0.471882 4_1:-2003361 Msa0529000:CDS
GAAACATTAAATTTATGACT+TGG 0.485653 4_1:+2003214 None:intergenic
AAAATAGTCATACATATTGA+AGG 0.494025 4_1:-2003612 Msa0529000:five_prime_UTR
CCAGAGTTGGTTGGAGTGGA+AGG 0.515293 4_1:-2003427 Msa0529000:CDS
TGTTCTCAGCTAAGAGCTCA+TGG 0.516002 4_1:-2003450 Msa0529000:intron
CCGACGACTTACGCAACGAC+AGG 0.517888 4_1:-2003327 Msa0529000:CDS
GTTGGTTGGAGTGGAAGGAA+AGG 0.527079 4_1:-2003422 Msa0529000:CDS
CAGTGTCATATATAATTCCT+TGG 0.530851 4_1:+2003080 None:intergenic
AGATCCTTCTAGCACAATGA+TGG 0.537596 4_1:+2003357 None:intergenic
ATGGCCAGAGTTGGTTGGAG+TGG 0.564909 4_1:-2003431 Msa0529000:CDS
AATTCAGAGGGAGAATCCAT+TGG 0.572843 4_1:-2003386 Msa0529000:CDS
TAAGAGCTCATGGCCAGAGT+TGG 0.573671 4_1:-2003440 Msa0529000:CDS
AGCTCATGGCCAGAGTTGGT+TGG 0.578782 4_1:-2003436 Msa0529000:CDS
TAAAGATGGAATTGTCACTC+AGG 0.589757 4_1:-2003287 Msa0529000:CDS
AGCAGAAGCTACAATTCAGA+GGG 0.593359 4_1:-2003398 Msa0529000:CDS
TAGCAGAAGCTACAATTCAG+AGG 0.596029 4_1:-2003399 Msa0529000:CDS
CCTGTCGTTGCGTAAGTCGT+CGG 0.616539 4_1:+2003327 None:intergenic
CGACGACTTACGCAACGACA+GGG 0.619969 4_1:-2003326 Msa0529000:CDS
CAATTGGATAGAAAGTGTCA+TGG 0.634335 4_1:-2003258 Msa0529000:exon
ACAATGATGGCATTCACCAA+TGG 0.650325 4_1:+2003370 None:intergenic
AAAATGTCTGATGAATGCAA+AGG 0.675933 4_1:-2003573 Msa0529000:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATATGAATAATAAATTAAA+TGG - chr4_1:2003209-2003228 Msa0529000:three_prime_UTR 10.0%
!! ATAAATTTAATGTTTCAATA+AGG - chr4_1:2003528-2003547 Msa0529000:intron 10.0%
!! TATAATAAGTAGTATACAAT+TGG + chr4_1:2003567-2003586 None:intergenic 15.0%
!! AATTGCAAAAAGAAAGAAAA+AGG - chr4_1:2003095-2003114 Msa0529000:three_prime_UTR 20.0%
!! AAAATAGTCATACATATTGA+AGG - chr4_1:2003123-2003142 Msa0529000:three_prime_UTR 20.0%
!! GAAACATTAAATTTATGACT+TGG + chr4_1:2003524-2003543 None:intergenic 20.0%
! TACATATTGAAGGATCAAAA+AGG - chr4_1:2003133-2003152 Msa0529000:three_prime_UTR 25.0%
!!! GTTTTTTATTTTCTATGCCA+AGG - chr4_1:2003638-2003657 Msa0529000:five_prime_UTR 25.0%
TTTGCAATTCTTCTTGTGTT+TGG + chr4_1:2003084-2003103 None:intergenic 30.0%
AAAATGTCTGATGAATGCAA+AGG - chr4_1:2003162-2003181 Msa0529000:three_prime_UTR 30.0%
CAGTGTCATATATAATTCCT+TGG + chr4_1:2003658-2003677 None:intergenic 30.0%
! TGGGTTTGGGTTAATAAAGA+TGG - chr4_1:2003434-2003453 Msa0529000:CDS 35.0%
TAAAGATGGAATTGTCACTC+AGG - chr4_1:2003448-2003467 Msa0529000:intron 35.0%
GACACTTTCTATCCAATTGT+TGG + chr4_1:2003476-2003495 None:intergenic 35.0%
!! CAATTGGATAGAAAGTGTCA+TGG - chr4_1:2003477-2003496 Msa0529000:intron 35.0%
GTCTGATGAATGCAAAGGTA+AGG - chr4_1:2003167-2003186 Msa0529000:three_prime_UTR 40.0%
TAGCAGAAGCTACAATTCAG+AGG - chr4_1:2003336-2003355 Msa0529000:CDS 40.0%
AGCAGAAGCTACAATTCAGA+GGG - chr4_1:2003337-2003356 Msa0529000:CDS 40.0%
AATTCAGAGGGAGAATCCAT+TGG - chr4_1:2003349-2003368 Msa0529000:CDS 40.0%
ACAATGATGGCATTCACCAA+TGG + chr4_1:2003368-2003387 None:intergenic 40.0%
AATGCCATCATTGTGCTAGA+AGG - chr4_1:2003374-2003393 Msa0529000:CDS 40.0%
AGATCCTTCTAGCACAATGA+TGG + chr4_1:2003381-2003400 None:intergenic 40.0%
TGTTCTCAGCTAAGAGCTCA+TGG - chr4_1:2003285-2003304 Msa0529000:CDS 45.0%
GTCACTCAGGTTCCAACAAT+TGG - chr4_1:2003461-2003480 Msa0529000:intron 45.0%
!!! AAATTTTTATTATAATTTGA+TGG - chr4_1:2003238-2003257 Msa0529000:three_prime_UTR 5.0%
TAAGAGCTCATGGCCAGAGT+TGG - chr4_1:2003295-2003314 Msa0529000:CDS 50.0%
GTTGGTTGGAGTGGAAGGAA+AGG - chr4_1:2003313-2003332 Msa0529000:CDS 50.0%
ACTTACGCAACGACAGGGTT+TGG - chr4_1:2003414-2003433 Msa0529000:CDS 50.0%
CTTACGCAACGACAGGGTTT+GGG - chr4_1:2003415-2003434 Msa0529000:CDS 50.0%
! CAACGACAGGGTTTGGGTTT+GGG - chr4_1:2003421-2003440 Msa0529000:CDS 50.0%
! AGCTCATGGCCAGAGTTGGT+TGG - chr4_1:2003299-2003318 Msa0529000:CDS 55.0%
! ATGGCCAGAGTTGGTTGGAG+TGG - chr4_1:2003304-2003323 Msa0529000:CDS 55.0%
CCTTCCACTCCAACCAACTC+TGG + chr4_1:2003311-2003330 None:intergenic 55.0%
! CCAGAGTTGGTTGGAGTGGA+AGG - chr4_1:2003308-2003327 Msa0529000:CDS 55.0%
CCTGTCGTTGCGTAAGTCGT+CGG + chr4_1:2003411-2003430 None:intergenic 55.0%
CGACGACTTACGCAACGACA+GGG - chr4_1:2003409-2003428 Msa0529000:CDS 55.0%
! GCAACGACAGGGTTTGGGTT+TGG - chr4_1:2003420-2003439 Msa0529000:CDS 55.0%
CCGACGACTTACGCAACGAC+AGG - chr4_1:2003408-2003427 Msa0529000:CDS 60.0%
Chromosome Type Strat End Strand Name
chr4_1 gene 2003064 2003693 2003064 ID=Msa0529000;Name=Msa0529000
chr4_1 mRNA 2003064 2003693 2003064 ID=Msa0529000-mRNA-1;Parent=Msa0529000;Name=Msa0529000-mRNA-1;_AED=0.04;_eAED=0.04;_QI=101|1|1|1|1|1|2|206|70
chr4_1 exon 2003064 2003463 2003064 ID=Msa0529000-mRNA-1:exon:644;Parent=Msa0529000-mRNA-1
chr4_1 exon 2003574 2003693 2003574 ID=Msa0529000-mRNA-1:exon:643;Parent=Msa0529000-mRNA-1
chr4_1 five_prime_UTR 2003593 2003693 2003593 ID=Msa0529000-mRNA-1:five_prime_utr;Parent=Msa0529000-mRNA-1
chr4_1 CDS 2003574 2003592 2003574 ID=Msa0529000-mRNA-1:cds;Parent=Msa0529000-mRNA-1
chr4_1 CDS 2003270 2003463 2003270 ID=Msa0529000-mRNA-1:cds;Parent=Msa0529000-mRNA-1
chr4_1 three_prime_UTR 2003064 2003269 2003064 ID=Msa0529000-mRNA-1:three_prime_utr;Parent=Msa0529000-mRNA-1
Gene Sequence

>Msa0529000

ATGTCTGATGAATGCAAAGCTAAGAGCTCATGGCCAGAGTTGGTTGGAGTGGAAGGAAAGGTAGCAGAAGCTACAATTCAGAGGGAGAATCCATTGGTGAATGCCATCATTGTGCTAGAAGGATCTTCTGTCACCGACGACTTACGCAACGACAGGGTTTGGGTTTGGGTTAATAAAGATGGAATTGTCACTCAGGTTCCAACAATTGGATAG

Protein sequence

>Msa0529000

MSDECKAKSSWPELVGVEGKVAEATIQRENPLVNAIIVLEGSSVTDDLRNDRVWVWVNKDGIVTQVPTIG*