AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1426610

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1426610 MtrunA17_Chr7g0275661 98.992 595 6 0 1 595 1 595 0.0 1212
Msa1426610 MtrunA17_Chr8g0346901 70.519 597 162 5 1 590 1 590 0.0 845
Msa1426610 MtrunA17_Chr3g0126781 56.966 567 221 7 35 591 25 578 0.0 641
Msa1426610 MtrunA17_Chr8g0388301 57.692 572 217 9 37 590 27 591 0.0 638
Msa1426610 MtrunA17_Chr5g0396531 55.478 575 226 10 29 590 23 580 0.0 629
Msa1426610 MtrunA17_Chr8g0366091 39.189 296 150 6 266 547 146 425 1.83e-59 209
Msa1426610 MtrunA17_Chr5g0420771 40.000 300 146 7 266 547 148 431 8.29e-59 207
Msa1426610 MtrunA17_Chr7g0255481 38.487 304 159 6 258 547 76 365 5.32e-57 198
Msa1426610 MtrunA17_Chr7g0220391 40.214 281 135 8 284 547 162 426 5.24e-55 197
Msa1426610 MtrunA17_Chr7g0274921 32.075 159 101 5 36 193 2 154 1.39e-14 73.6
Msa1426610 MtrunA17_Chr7g0274921 31.646 158 101 5 37 193 79 230 4.98e-14 72.0
Msa1426610 MtrunA17_Chr6g0475961 32.051 156 99 5 37 191 4 153 1.68e-14 72.4
Msa1426610 MtrunA17_Chr1g0181441 31.646 158 101 5 37 193 1 152 3.99e-14 72.4
Msa1426610 MtrunA17_Chr1g0181441 31.646 158 101 5 37 193 77 228 3.99e-14 72.4
Msa1426610 MtrunA17_Chr4g0041751 31.013 158 102 5 37 193 1 152 4.79e-14 72.0
Msa1426610 MtrunA17_Chr4g0037241 33.333 135 84 4 37 170 1 130 1.71e-13 68.6
Msa1426610 MtrunA17_Chr5g0444101 33.333 135 84 4 37 170 1 130 1.71e-13 68.6
Msa1426610 MtrunA17_Chr8g0377821 31.646 158 101 5 37 193 9 160 2.21e-13 72.4
Msa1426610 MtrunA17_Chr8g0377821 31.646 158 101 5 37 193 85 236 2.21e-13 72.4
Msa1426610 MtrunA17_Chr8g0377821 31.646 158 101 5 37 193 161 312 2.21e-13 72.4
Msa1426610 MtrunA17_Chr8g0377821 31.646 158 101 5 37 193 237 388 2.21e-13 72.4
Msa1426610 MtrunA17_Chr4g0046801 31.447 159 102 5 36 193 3 155 2.31e-13 72.8
Msa1426610 MtrunA17_Chr4g0046801 31.646 158 101 5 37 193 80 231 3.38e-13 72.0
Msa1426610 MtrunA17_Chr4g0046801 31.646 158 101 5 37 193 156 307 3.38e-13 72.0
Msa1426610 MtrunA17_Chr4g0046801 31.646 158 101 5 37 193 232 383 3.38e-13 72.0
Msa1426610 MtrunA17_Chr4g0046801 31.646 158 101 5 37 193 308 459 3.38e-13 72.0
Msa1426610 MtrunA17_Chr4g0041641 31.646 158 101 5 37 193 5 156 2.89e-13 72.4
Msa1426610 MtrunA17_Chr4g0041641 31.646 158 101 5 37 193 81 232 2.89e-13 72.4
Msa1426610 MtrunA17_Chr4g0041641 31.646 158 101 5 37 193 157 308 2.89e-13 72.4
Msa1426610 MtrunA17_Chr4g0041641 31.646 158 101 5 37 193 233 384 2.89e-13 72.4
Msa1426610 MtrunA17_Chr4g0041641 31.646 158 101 5 37 193 309 460 2.89e-13 72.4
Msa1426610 MtrunA17_Chr4g0048591 31.447 159 102 5 36 193 3 155 3.05e-13 72.4
Msa1426610 MtrunA17_Chr4g0048591 31.646 158 101 5 37 193 232 383 3.83e-13 72.0
Msa1426610 MtrunA17_Chr4g0048591 31.646 158 101 5 37 193 80 231 4.44e-13 72.0
Msa1426610 MtrunA17_Chr4g0048591 31.646 158 101 5 37 193 156 307 4.44e-13 72.0
Msa1426610 MtrunA17_Chr4g0048591 31.646 158 101 5 37 193 384 535 4.44e-13 72.0
Msa1426610 MtrunA17_Chr8g0342711 31.646 158 101 5 37 193 4 155 4.10e-13 72.0
Msa1426610 MtrunA17_Chr8g0342711 31.646 158 101 5 37 193 80 231 4.10e-13 72.0
Msa1426610 MtrunA17_Chr8g0342711 31.646 158 101 5 37 193 156 307 4.10e-13 72.0
Msa1426610 MtrunA17_Chr8g0342711 31.646 158 101 5 37 193 232 383 4.10e-13 72.0
Msa1426610 MtrunA17_Chr8g0342711 31.646 158 101 5 37 193 308 459 4.10e-13 72.0
Msa1426610 MtrunA17_Chr8g0342711 31.646 158 101 5 37 193 384 535 4.10e-13 72.0
Msa1426610 MtrunA17_Chr4g0041701 31.646 158 101 5 37 193 1 152 4.26e-13 72.0
Msa1426610 MtrunA17_Chr4g0041701 31.646 158 101 5 37 193 77 228 4.26e-13 72.0
Msa1426610 MtrunA17_Chr4g0041701 31.646 158 101 5 37 193 153 304 4.26e-13 72.0
Msa1426610 MtrunA17_Chr4g0041701 31.646 158 101 5 37 193 229 380 4.26e-13 72.0
Msa1426610 MtrunA17_Chr4g0041701 31.646 158 101 5 37 193 305 456 4.26e-13 72.0
Msa1426610 MtrunA17_Chr4g0041701 31.646 158 101 5 37 193 381 532 4.26e-13 72.0
Msa1426610 MtrunA17_Chr4g0046831 31.646 158 101 5 37 193 80 231 5.30e-13 72.0
Msa1426610 MtrunA17_Chr4g0046831 31.646 158 101 5 37 193 156 307 5.30e-13 72.0
Msa1426610 MtrunA17_Chr4g0046831 31.646 158 101 5 37 193 368 519 5.30e-13 72.0
Msa1426610 MtrunA17_Chr4g0046831 31.646 158 101 5 37 193 444 595 5.30e-13 72.0
Msa1426610 MtrunA17_Chr4g0046831 31.646 158 101 5 37 193 4 155 5.98e-13 71.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1426610 AT2G46500.1 58.615 592 210 8 1 588 1 561 0.0 687
Msa1426610 AT2G46500.2 58.615 592 210 8 1 588 1 561 0.0 687
Msa1426610 AT5G24240.1 55.160 562 225 8 34 589 30 570 0.0 616
Msa1426610 AT5G24240.2 55.160 562 225 8 34 589 30 570 0.0 616
Msa1426610 AT2G46500.3 51.773 423 170 7 1 419 1 393 2.37e-142 419
Msa1426610 AT2G03890.1 38.739 333 168 9 254 566 139 455 4.05e-59 209
Msa1426610 AT1G13640.1 39.404 302 146 7 267 547 144 429 5.00e-57 202
Msa1426610 AT1G13640.2 39.404 302 146 7 267 547 144 429 5.00e-57 202
Msa1426610 AT3G56600.2 36.503 326 174 7 257 562 72 384 1.50e-56 199
Msa1426610 AT3G56600.1 36.503 326 174 7 257 562 75 387 1.66e-56 199
Msa1426610 AT3G56600.3 36.503 326 174 7 257 562 75 387 1.66e-56 199
Msa1426610 AT2G40850.1 36.716 335 177 8 259 572 94 414 9.35e-56 198
Msa1426610 AT1G26270.1 38.333 300 152 8 265 547 146 429 1.17e-55 199
Msa1426610 AT2G03890.2 41.304 230 104 6 334 547 99 313 7.46e-45 167
Msa1426610 AT1G27570.1 30.806 211 128 5 344 543 396 599 2.74e-22 101
Msa1426610 AT1G27570.2 30.806 211 128 5 344 543 387 590 3.17e-22 101
Msa1426610 AT4G05320.7 32.051 156 99 5 37 191 1 150 7.71e-15 72.8
Msa1426610 AT4G05050.4 32.051 156 99 5 37 191 1 150 7.71e-15 72.8
Msa1426610 AT2G35635.1 32.051 156 99 5 37 191 1 150 3.52e-14 70.9
Msa1426610 AT4G02890.2 31.646 158 101 5 37 193 1 152 5.87e-14 72.0
Msa1426610 AT4G02890.2 31.646 158 101 5 37 193 77 228 5.87e-14 72.0
Msa1426610 AT4G02890.1 31.646 158 101 5 37 193 1 152 5.87e-14 72.0
Msa1426610 AT4G02890.1 31.646 158 101 5 37 193 77 228 5.87e-14 72.0
Msa1426610 AT4G05050.2 31.646 158 101 5 37 193 1 152 5.87e-14 72.0
Msa1426610 AT4G05050.2 31.646 158 101 5 37 193 77 228 5.87e-14 72.0
Msa1426610 AT4G05050.1 31.646 158 101 5 37 193 1 152 5.87e-14 72.0
Msa1426610 AT4G05050.1 31.646 158 101 5 37 193 77 228 5.87e-14 72.0
Msa1426610 AT4G05050.3 31.646 158 101 5 37 193 1 152 5.87e-14 72.0
Msa1426610 AT4G05050.3 31.646 158 101 5 37 193 77 228 5.87e-14 72.0
Msa1426610 AT1G31340.1 32.051 156 99 5 37 191 1 150 7.24e-14 70.1
Msa1426610 AT3G43950.1 34.815 135 79 4 347 478 78 206 8.47e-14 71.2
Msa1426610 AT5G03240.3 31.646 158 101 5 37 193 1 152 1.75e-13 72.0
Msa1426610 AT5G03240.3 31.646 158 101 5 37 193 77 228 1.75e-13 72.0
Msa1426610 AT5G03240.3 31.646 158 101 5 37 193 153 304 1.75e-13 72.0
Msa1426610 AT5G03240.2 31.646 158 101 5 37 193 1 152 1.75e-13 72.0
Msa1426610 AT5G03240.2 31.646 158 101 5 37 193 77 228 1.75e-13 72.0
Msa1426610 AT5G03240.2 31.646 158 101 5 37 193 153 304 1.75e-13 72.0
Msa1426610 AT5G03240.1 31.646 158 101 5 37 193 1 152 1.75e-13 72.0
Msa1426610 AT5G03240.1 31.646 158 101 5 37 193 77 228 1.75e-13 72.0
Msa1426610 AT5G03240.1 31.646 158 101 5 37 193 153 304 1.75e-13 72.0
Msa1426610 AT5G20620.2 31.646 158 101 5 37 193 1 152 1.75e-13 72.0
Msa1426610 AT5G20620.2 31.646 158 101 5 37 193 77 228 1.75e-13 72.0
Msa1426610 AT5G20620.2 31.646 158 101 5 37 193 153 304 1.75e-13 72.0
Msa1426610 AT4G05320.5 31.646 158 101 5 37 193 1 152 1.76e-13 72.0
Msa1426610 AT4G05320.5 31.646 158 101 5 37 193 77 228 1.76e-13 72.0
Msa1426610 AT4G05320.5 31.646 158 101 5 37 193 153 304 1.76e-13 72.0
Msa1426610 AT4G05320.8 31.646 158 101 5 37 193 1 152 1.76e-13 72.0
Msa1426610 AT4G05320.8 31.646 158 101 5 37 193 77 228 1.76e-13 72.0
Msa1426610 AT4G05320.8 31.646 158 101 5 37 193 153 304 1.76e-13 72.0
Msa1426610 AT4G02890.3 31.646 158 101 5 37 193 1 152 1.76e-13 72.0
Msa1426610 AT4G02890.3 31.646 158 101 5 37 193 77 228 1.76e-13 72.0
Msa1426610 AT4G02890.3 31.646 158 101 5 37 193 153 304 1.76e-13 72.0
Msa1426610 AT4G02890.4 31.646 158 101 5 37 193 1 152 1.76e-13 72.0
Msa1426610 AT4G02890.4 31.646 158 101 5 37 193 77 228 1.76e-13 72.0
Msa1426610 AT4G02890.4 31.646 158 101 5 37 193 153 304 1.76e-13 72.0
Msa1426610 AT1G65350.1 32.278 158 99 5 37 193 1 151 2.15e-13 72.0
Msa1426610 AT1G65350.1 29.114 158 104 5 37 193 77 227 2.23e-11 65.9
Msa1426610 AT1G55060.1 29.651 172 104 5 33 193 63 228 2.26e-13 70.5
Msa1426610 AT5G20620.1 31.646 158 101 5 37 193 1 152 3.28e-13 72.0
Msa1426610 AT5G20620.1 31.646 158 101 5 37 193 77 228 3.28e-13 72.0
Msa1426610 AT5G20620.1 31.646 158 101 5 37 193 153 304 3.28e-13 72.0
Msa1426610 AT5G20620.1 31.646 158 101 5 37 193 229 380 3.28e-13 72.0
Msa1426610 AT4G05320.1 31.646 158 101 5 37 193 1 152 3.60e-13 72.0
Msa1426610 AT4G05320.1 31.646 158 101 5 37 193 77 228 3.60e-13 72.0
Msa1426610 AT4G05320.1 31.646 158 101 5 37 193 153 304 3.60e-13 72.0
Msa1426610 AT4G05320.1 31.646 158 101 5 37 193 229 380 3.60e-13 72.0
Msa1426610 AT4G05320.6 31.646 158 101 5 37 193 1 152 3.60e-13 72.0
Msa1426610 AT4G05320.6 31.646 158 101 5 37 193 77 228 3.60e-13 72.0
Msa1426610 AT4G05320.6 31.646 158 101 5 37 193 153 304 3.60e-13 72.0
Msa1426610 AT4G05320.6 31.646 158 101 5 37 193 229 380 3.60e-13 72.0
Msa1426610 AT4G05320.3 31.646 158 101 5 37 193 1 152 3.60e-13 72.0
Msa1426610 AT4G05320.3 31.646 158 101 5 37 193 77 228 3.60e-13 72.0
Msa1426610 AT4G05320.3 31.646 158 101 5 37 193 153 304 3.60e-13 72.0
Msa1426610 AT4G05320.3 31.646 158 101 5 37 193 229 380 3.60e-13 72.0
Msa1426610 AT4G05320.2 31.646 158 101 5 37 193 1 152 4.58e-13 72.0
Msa1426610 AT4G05320.2 31.646 158 101 5 37 193 77 228 4.58e-13 72.0
Msa1426610 AT4G05320.2 31.646 158 101 5 37 193 153 304 4.58e-13 72.0
Msa1426610 AT4G05320.2 31.646 158 101 5 37 193 229 380 4.58e-13 72.0
Msa1426610 AT4G05320.2 31.646 158 101 5 37 193 305 456 4.58e-13 72.0
Msa1426610 AT4G05320.4 31.646 158 101 5 37 193 1 152 4.58e-13 72.0
Msa1426610 AT4G05320.4 31.646 158 101 5 37 193 77 228 4.58e-13 72.0
Msa1426610 AT4G05320.4 31.646 158 101 5 37 193 153 304 4.58e-13 72.0
Msa1426610 AT4G05320.4 31.646 158 101 5 37 193 229 380 4.58e-13 72.0
Msa1426610 AT4G05320.4 31.646 158 101 5 37 193 305 456 4.58e-13 72.0
Msa1426610 AT5G37640.1 29.518 166 105 5 52 211 19 178 1.24e-11 66.6

Find 156 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTATCAGCTTGTTGAATTT+AGG 0.133656 tig0026054:+40268 Msa1426610:five_prime_UTR
AACTTGCGACGTCCACTTAT+TGG 0.166902 tig0026054:-41694 None:intergenic
GAGGTAAAATTGGTCTCTTT+TGG 0.196561 tig0026054:+40315 Msa1426610:five_prime_UTR
CATACTCCTACCTAGCTTAA+AGG 0.229628 tig0026054:-40405 None:intergenic
AGCTTCAGTGATTCGAAATA+TGG 0.237481 tig0026054:+41417 Msa1426610:CDS
TTTGATCTTTGAGGTAAAAT+TGG 0.241624 tig0026054:+40305 Msa1426610:five_prime_UTR
TCTCTATCGGGAAGCTTTCT+TGG 0.255627 tig0026054:-41352 None:intergenic
GGAAGTTGCTGCTTATGTTT+TGG 0.257385 tig0026054:+41666 Msa1426610:CDS
AGTGGACGTCGCAAGTTATT+TGG 0.265630 tig0026054:+41700 Msa1426610:CDS
CAAGCGAAGGATTGCTAAAA+AGG 0.268222 tig0026054:+41048 Msa1426610:CDS
TGCATCTGTTTGTTCGAAAA+AGG 0.284524 tig0026054:+41179 Msa1426610:CDS
TTATCAGCTTGTTGAATTTA+GGG 0.288456 tig0026054:+40269 Msa1426610:five_prime_UTR
TTCAATATTTCTCTCGTTCT+CGG 0.303158 tig0026054:+40739 Msa1426610:CDS
CCGTATTCTGCTGAAACTAT+TGG 0.313231 tig0026054:+42478 Msa1426610:CDS
AGTGTAAACAAAGGTTATTT+TGG 0.322758 tig0026054:+40568 Msa1426610:five_prime_UTR
CAGAATACGGCTGGCGAGCT+TGG 0.326056 tig0026054:-42465 None:intergenic
GATACTCTACTTAAGGATTA+TGG 0.332495 tig0026054:+40899 Msa1426610:CDS
ATCTTCTAATTTGATCTTTG+AGG 0.333431 tig0026054:+40296 Msa1426610:five_prime_UTR
CCTTCACCGTCTAATGATAA+TGG 0.336135 tig0026054:-41604 None:intergenic
AGAATACGGCTGGCGAGCTT+GGG 0.336231 tig0026054:-42464 None:intergenic
GCATTTGAGTCTGTTCTTCC+TGG 0.336546 tig0026054:+42712 Msa1426610:CDS
AAGACAAGTGAGATGGTAAA+TGG 0.339437 tig0026054:-40700 None:intergenic
ATGAGTGTCGCTTTAGGCTT+TGG 0.343024 tig0026054:+42976 Msa1426610:three_prime_UTR
AGCAGGAACTTGTGTGTAAT+GGG 0.346405 tig0026054:+41095 Msa1426610:CDS
AGCTGTGAAGATATCGGTCC+TGG 0.357604 tig0026054:+41850 Msa1426610:CDS
ATCAATGTCAAAACATCTTC+GGG 0.363524 tig0026054:+40977 Msa1426610:CDS
AAAGTTTGAGAGGGTAAAGT+TGG 0.364705 tig0026054:+43114 Msa1426610:three_prime_UTR
TACTTTATGCTTGATTCAAC+TGG 0.367790 tig0026054:+41511 Msa1426610:CDS
GGACAATGACCAGTCTGTTC+TGG 0.368416 tig0026054:+41972 Msa1426610:CDS
TTGATATCTGCAATAGTAAC+AGG 0.371471 tig0026054:+42823 Msa1426610:three_prime_UTR
TTCAAAATGAGTGTCGCTTT+AGG 0.377610 tig0026054:+42970 Msa1426610:three_prime_UTR
TAACCATCCAGGAGACTTGA+TGG 0.377723 tig0026054:+41786 Msa1426610:CDS
ATGGAAGACTCTCACACCTT+TGG 0.377727 tig0026054:-40823 None:intergenic
CGTATTCTGCTGAAACTATT+GGG 0.383361 tig0026054:+42479 Msa1426610:CDS
GTACCTTCTGCAGATCCTAT+TGG 0.386108 tig0026054:-41478 None:intergenic
GGTTGAGCTGTTTGTTATGA+TGG 0.387025 tig0026054:+40488 Msa1426610:five_prime_UTR
CATCAATGTCAAAACATCTT+CGG 0.391195 tig0026054:+40976 Msa1426610:CDS
ATAATAATATTTCTACAATT+AGG 0.397748 tig0026054:-40461 None:intergenic
ATCAAGTTTCCAATTGCAAA+AGG 0.400969 tig0026054:-42637 None:intergenic
GTTATTGAATTGAAATTTGT+AGG 0.401634 tig0026054:-40184 None:intergenic
AATTAAATGCTTACACTTGT+GGG 0.404724 tig0026054:-42021 None:intergenic
TAGTTGGTCAAGGAGCGTTC+AGG 0.416347 tig0026054:+41644 Msa1426610:CDS
AAATGGAATGAAATAATCAT+TGG 0.416426 tig0026054:+43012 Msa1426610:three_prime_UTR
GATGGTAAATGGAATGCATT+TGG 0.428711 tig0026054:-40689 None:intergenic
TTTAACTAACAAACAGAAGT+TGG 0.431926 tig0026054:+40847 Msa1426610:CDS
TGGTGTTCCTCCAACATTGA+TGG 0.433858 tig0026054:+41741 Msa1426610:CDS
TCTTGGCACCGCTCTCTGAA+TGG 0.434707 tig0026054:-41335 None:intergenic
TCAATATTTCTCTCGTTCTC+GGG 0.438311 tig0026054:+40740 Msa1426610:CDS
TCGAAGTGATACTCTACTTA+AGG 0.438824 tig0026054:+40892 Msa1426610:CDS
GATGGTCAAGTGCTTGCATA+AGG 0.443223 tig0026054:+41759 Msa1426610:CDS
AGCTTCCACCTTTCGATCTA+CGG 0.445912 tig0026054:+40239 Msa1426610:five_prime_UTR
CAATTGGAAACTTGATGTGC+AGG 0.450882 tig0026054:+42644 Msa1426610:CDS
AGAGTTCGAGCACATTCAAT+TGG 0.454661 tig0026054:-42565 None:intergenic
GAACGAGAGAAATATTGAAA+TGG 0.459276 tig0026054:-40735 None:intergenic
GTTCTGGTTCCAATTGATCA+TGG 0.461509 tig0026054:+41988 Msa1426610:CDS
ATCTTAGCCATCAAGTCTCC+TGG 0.464083 tig0026054:-41793 None:intergenic
CTCCAAAAGAAAATCTCTAT+CGG 0.467054 tig0026054:-41365 None:intergenic
GTTTGATGATCTTGAGGAGC+AGG 0.472677 tig0026054:+41078 Msa1426610:CDS
GCTAGCGAATACTATCCAAT+AGG 0.473031 tig0026054:+41463 Msa1426610:CDS
TAATGATAATGGAAGACCTC+GGG 0.474414 tig0026054:-41593 None:intergenic
TTGAAGAAAGGGGTTGAGAG+AGG 0.478531 tig0026054:+42607 Msa1426610:CDS
TTGGCACACAGATTAAGAAA+TGG 0.480404 tig0026054:+42995 Msa1426610:three_prime_UTR
CCAATAGTTTCAGCAGAATA+CGG 0.480969 tig0026054:-42478 None:intergenic
TTGAGCAATTAGTGCAAAAG+AGG 0.481203 tig0026054:-40214 None:intergenic
TCCCATTCCATAACACGAAA+AGG 0.482974 tig0026054:-40773 None:intergenic
AAATTAAATGCTTACACTTG+TGG 0.484991 tig0026054:-42022 None:intergenic
CATAAGGGATTTAACCATCC+AGG 0.485124 tig0026054:+41775 Msa1426610:CDS
AAGCAGTATCCATGATCAAT+TGG 0.485316 tig0026054:-41997 None:intergenic
AGTTGGTCAAGGAGCGTTCA+GGG 0.486553 tig0026054:+41645 Msa1426610:CDS
GAACAAGGAATCTGTGATAG+AGG 0.486875 tig0026054:+42675 Msa1426610:CDS
TTAGTGCAAAAGAGGGTAAC+GGG 0.487025 tig0026054:-40206 None:intergenic
TCAAGTTTCCAATTGCAAAA+GGG 0.487387 tig0026054:-42636 None:intergenic
AACCCCTTTCTTCAAAAGCA+TGG 0.489309 tig0026054:-42599 None:intergenic
AATGGCTCAGTGGGATCCAC+TGG 0.494737 tig0026054:-40659 None:intergenic
GGAGACTTGATGGCTAAGAT+TGG 0.495959 tig0026054:+41796 Msa1426610:CDS
ATAGGATCTGCAGAAGGTAC+CGG 0.497760 tig0026054:+41481 Msa1426610:CDS
TGTAGAAATATTATTATGCA+TGG 0.497787 tig0026054:+40467 Msa1426610:five_prime_UTR
TATCACTTCGAGCGAGTTCA+CGG 0.499648 tig0026054:-40880 None:intergenic
AGATGCTGTTTCTCAAATCA+TGG 0.499783 tig0026054:+42756 Msa1426610:CDS
TTCTTGTGGAAGAAAATTTG+AGG 0.501544 tig0026054:+41288 Msa1426610:CDS
GCAAATGCAGATAGGCATGC+TGG 0.503643 tig0026054:+41925 Msa1426610:CDS
AAACAGAAGTTGGTTTATGC+TGG 0.505699 tig0026054:+40857 Msa1426610:CDS
AGTACACAAAATTAGTGTAC+TGG 0.506035 tig0026054:+41891 Msa1426610:CDS
CAAGTTATTTGGTGATGTGA+AGG 0.508171 tig0026054:+41711 Msa1426610:CDS
TCCAAAAGAAAATCTCTATC+GGG 0.509865 tig0026054:-41364 None:intergenic
GCCATTGGCTCTTCGTCGAT+AGG 0.511038 tig0026054:-41559 None:intergenic
TAGTGTACTGGACATAAGAT+TGG 0.513283 tig0026054:+41903 Msa1426610:CDS
GAGTAAGTTATAACAAAGAA+TGG 0.513459 tig0026054:-42940 None:intergenic
GAGAGTTGAAAATCTTATCA+AGG 0.515143 tig0026054:-42786 None:intergenic
TAAGATTGGCAAATGCAGAT+AGG 0.515290 tig0026054:+41917 Msa1426610:CDS
CAAGTTTCCAATTGCAAAAG+GGG 0.516578 tig0026054:-42635 None:intergenic
CCATTATCATTAGACGGTGA+AGG 0.523513 tig0026054:+41604 Msa1426610:CDS
TAGGATCTGCAGAAGGTACC+GGG 0.527750 tig0026054:+41482 Msa1426610:CDS
TGTGGAAGAAAATTTGAGGT+TGG 0.531735 tig0026054:+41292 Msa1426610:CDS
GTGTAATGGGGAACGGTTGG+AGG 0.532109 tig0026054:+41108 Msa1426610:CDS
AAGTTATTTGGTGATGTGAA+GGG 0.535843 tig0026054:+41712 Msa1426610:CDS
CACCGCTCTCTGAATGGCAT+CGG 0.538669 tig0026054:-41329 None:intergenic
GCTGTGAAGATATCGGTCCT+GGG 0.539152 tig0026054:+41851 Msa1426610:CDS
GAAACAGTTTGATGATCTTG+AGG 0.541679 tig0026054:+41072 Msa1426610:CDS
TCATTTACCGGGAAAGCCCC+AGG 0.542503 tig0026054:-41868 None:intergenic
GGAAGAAAATTTGAGGTTGG+TGG 0.542977 tig0026054:+41295 Msa1426610:CDS
GAGCAGGAACTTGTGTGTAA+TGG 0.543660 tig0026054:+41094 Msa1426610:CDS
AATGTAGCTTCACTTGTGCC+AGG 0.546538 tig0026054:-42730 None:intergenic
TATTGGGTACATAAAGTCAC+TGG 0.549010 tig0026054:+42495 Msa1426610:CDS
TAGTTTCAGCAGAATACGGC+TGG 0.549860 tig0026054:-42474 None:intergenic
GGAGACAACAATGGCTCAGT+GGG 0.550782 tig0026054:-40668 None:intergenic
TGAAAATCTTATCAAGGCGC+TGG 0.555048 tig0026054:-42780 None:intergenic
TGTGAGAGAAAAGTTTGAGA+GGG 0.557080 tig0026054:+43105 Msa1426610:three_prime_UTR
ATGGTCAAGTGCTTGCATAA+GGG 0.559797 tig0026054:+41760 Msa1426610:CDS
AATGGGAGCTGTGAAGATAT+CGG 0.559895 tig0026054:+41844 Msa1426610:CDS
GCCTATCGACGAAGAGCCAA+TGG 0.563365 tig0026054:+41558 Msa1426610:CDS
GTTACAATGGACAACTCAAA+GGG 0.565579 tig0026054:-41223 None:intergenic
AAGGATTATGGAGTAACTGA+TGG 0.571025 tig0026054:+40911 Msa1426610:CDS
AGTTACAATGGACAACTCAA+AGG 0.571959 tig0026054:-41224 None:intergenic
CAAATGCAGATAGGCATGCT+GGG 0.572744 tig0026054:+41926 Msa1426610:CDS
GTGTGAGAGAAAAGTTTGAG+AGG 0.572922 tig0026054:+43104 Msa1426610:three_prime_UTR
CACTTGACCATCAATGTTGG+AGG 0.573274 tig0026054:-41748 None:intergenic
GAATACGGCTGGCGAGCTTG+GGG 0.573463 tig0026054:-42463 None:intergenic
TGTGTGTAATGGGGAACGGT+TGG 0.578859 tig0026054:+41105 Msa1426610:CDS
AAGCATAAAGTAAGCTCCCC+CGG 0.581274 tig0026054:-41500 None:intergenic
ATTAGTGCAAAAGAGGGTAA+CGG 0.581518 tig0026054:-40207 None:intergenic
GTCCATTGTAACTACAGACT+TGG 0.582693 tig0026054:+41234 Msa1426610:CDS
TGTTAGTACTCTTAGTCCAG+TGG 0.584156 tig0026054:+40643 Msa1426610:CDS
TGAGCAATTAGTGCAAAAGA+GGG 0.585294 tig0026054:-40213 None:intergenic
TTGATTCTCAGTGTAAACAA+AGG 0.588245 tig0026054:+40559 Msa1426610:five_prime_UTR
GGTCTTCCATTATCATTAGA+CGG 0.588994 tig0026054:+41598 Msa1426610:CDS
TCCTCAAAAGACAAGTGAGA+TGG 0.590743 tig0026054:-40707 None:intergenic
ATATGTATAGCTCAAAACAT+TGG 0.592974 tig0026054:+42866 Msa1426610:three_prime_UTR
AGCCAAGTCTGTAGTTACAA+TGG 0.593929 tig0026054:-41236 None:intergenic
AAGGGTACAATAGTTGGTCA+AGG 0.598430 tig0026054:+41634 Msa1426610:CDS
TTAGTGAAAATGTCATCTGC+TGG 0.606185 tig0026054:+40620 Msa1426610:exon
ATGTAAAATACATCAAGCGA+AGG 0.618575 tig0026054:+41035 Msa1426610:CDS
AAACGACACCGTAGATCGAA+AGG 0.619213 tig0026054:-40247 None:intergenic
ATGGTAAACTCTGCATACGA+TGG 0.619677 tig0026054:+41436 Msa1426610:CDS
AAGCACTTGACCATCAATGT+TGG 0.627426 tig0026054:-41751 None:intergenic
TACCGATGCCATTCAGAGAG+CGG 0.639607 tig0026054:+41327 Msa1426610:CDS
CTGTGAAGATATCGGTCCTG+GGG 0.640174 tig0026054:+41852 Msa1426610:CDS
TAGCCATCAAGTCTCCTGGA+TGG 0.640520 tig0026054:-41789 None:intergenic
CTGCAAGCATAATGATGCAG+TGG 0.641116 tig0026054:+41156 Msa1426610:CDS
CTAATGATAATGGAAGACCT+CGG 0.646268 tig0026054:-41594 None:intergenic
AACTTGTGTGTAATGGGGAA+CGG 0.646813 tig0026054:+41101 Msa1426610:CDS
TGGAGACAACAATGGCTCAG+TGG 0.649706 tig0026054:-40669 None:intergenic
AATTGGAAACTTGATGTGCA+GGG 0.653610 tig0026054:+42645 Msa1426610:CDS
GTGCAGGGAATCATTGAACA+AGG 0.654835 tig0026054:+42660 Msa1426610:CDS
CTGTTAAGTTCAAAATCCAA+AGG 0.656079 tig0026054:+40807 Msa1426610:CDS
AATGCATTTGGAGACAACAA+TGG 0.660019 tig0026054:-40677 None:intergenic
AATGATAATGGAAGACCTCG+GGG 0.661241 tig0026054:-41592 None:intergenic
CAATTGGAACCAGAACAGAC+TGG 0.671066 tig0026054:-41981 None:intergenic
AAACTCAATGATGATGACGA+TGG 0.676648 tig0026054:-40156 None:intergenic
AGGATCTGCAGAAGGTACCG+GGG 0.678821 tig0026054:+41483 Msa1426610:CDS
GCAGGAACTTGTGTGTAATG+GGG 0.680555 tig0026054:+41096 Msa1426610:CDS
TATCCAATAGGATCTGCAGA+AGG 0.683129 tig0026054:+41475 Msa1426610:CDS
CGACACCGTAGATCGAAAGG+TGG 0.695095 tig0026054:-40244 None:intergenic
TCAATGTCAAAACATCTTCG+GGG 0.701785 tig0026054:+40978 Msa1426610:CDS
GGATCTGCAGAAGGTACCGG+GGG 0.709644 tig0026054:+41484 Msa1426610:CDS
ATGATAATGGAAGACCTCGG+GGG 0.745031 tig0026054:-41591 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATAATATTTCTACAATT+AGG - tig0026054:40464-40483 None:intergenic 10.0%
!! TTATGACTTAAAAAATGAAT+TGG + tig0026054:42278-42297 Msa1426610:intron 15.0%
!! GTTATTGAATTGAAATTTGT+AGG - tig0026054:40187-40206 None:intergenic 20.0%
!! AAAAACTAATACGAACAAAA+AGG - tig0026054:40378-40397 None:intergenic 20.0%
!! TGTAGAAATATTATTATGCA+TGG + tig0026054:40467-40486 Msa1426610:five_prime_UTR 20.0%
!!! TTCTGTTTGTTAGTTAAAAA+TGG - tig0026054:40845-40864 None:intergenic 20.0%
!!! ATTTTAGGGTTAACTATAAT+TGG - tig0026054:41391-41410 None:intergenic 20.0%
!!! AACATAATGTTTTCAAATAG+TGG + tig0026054:42104-42123 Msa1426610:intron 20.0%
!! AAATGGAATGAAATAATCAT+TGG + tig0026054:43012-43031 Msa1426610:three_prime_UTR 20.0%
!!! TGTCTTTTTCTAATAAAGTA+AGG - tig0026054:43061-43080 None:intergenic 20.0%
!! GAAAAAAATTAAAGTTGCAT+TGG + tig0026054:43139-43158 Msa1426610:three_prime_UTR 20.0%
!!! TTTATCAGCTTGTTGAATTT+AGG + tig0026054:40268-40287 Msa1426610:five_prime_UTR 25.0%
!!! TTATCAGCTTGTTGAATTTA+GGG + tig0026054:40269-40288 Msa1426610:five_prime_UTR 25.0%
! ATCTTCTAATTTGATCTTTG+AGG + tig0026054:40296-40315 Msa1426610:five_prime_UTR 25.0%
!!! TTTGATCTTTGAGGTAAAAT+TGG + tig0026054:40305-40324 Msa1426610:five_prime_UTR 25.0%
!! AAAAAGGAAAGATCTTTTGA+AGG - tig0026054:40362-40381 None:intergenic 25.0%
! TTTGAAAAAACCTTTAAGCT+AGG + tig0026054:40395-40414 Msa1426610:five_prime_UTR 25.0%
!!! ATTTGTAGACTTTAGTTTTG+AGG + tig0026054:40523-40542 Msa1426610:five_prime_UTR 25.0%
!!! TTTGTAGACTTTAGTTTTGA+GGG + tig0026054:40524-40543 Msa1426610:five_prime_UTR 25.0%
!! AGTGTAAACAAAGGTTATTT+TGG + tig0026054:40568-40587 Msa1426610:five_prime_UTR 25.0%
! TTTAACTAACAAACAGAAGT+TGG + tig0026054:40847-40866 Msa1426610:CDS 25.0%
!!! GATGTTTTTGAAAATTCTTG+TGG + tig0026054:41274-41293 Msa1426610:CDS 25.0%
!! TTAAAAAAGGGTACAATAGT+TGG + tig0026054:41628-41647 Msa1426610:CDS 25.0%
! AGATGTTTGTGAAAAACAAT+GGG + tig0026054:41827-41846 Msa1426610:CDS 25.0%
!! ATTTTGTGTACTTCATTTAC+CGG - tig0026054:41883-41902 None:intergenic 25.0%
! AATTAAATGCTTACACTTGT+GGG - tig0026054:42024-42043 None:intergenic 25.0%
! AAATTAAATGCTTACACTTG+TGG - tig0026054:42025-42044 None:intergenic 25.0%
!! TTTTCAAATAGTGGTTATAG+CGG + tig0026054:42113-42132 Msa1426610:intron 25.0%
! TTTGTGCAATCTACAATATA+TGG + tig0026054:42242-42261 Msa1426610:intron 25.0%
! ACTTAAAAAATGAATTGGCT+AGG + tig0026054:42283-42302 Msa1426610:intron 25.0%
! TGTATTGAAATAATGAAGTC+TGG + tig0026054:42324-42343 Msa1426610:intron 25.0%
!! TTGAAGATTGTACTTTTGAA+TGG + tig0026054:42431-42450 Msa1426610:CDS 25.0%
! ATATGTATAGCTCAAAACAT+TGG + tig0026054:42866-42885 Msa1426610:three_prime_UTR 25.0%
!!! GGATTTTTAGTTCATAACAA+CGG + tig0026054:42887-42906 Msa1426610:three_prime_UTR 25.0%
! GAGTAAGTTATAACAAAGAA+TGG - tig0026054:42943-42962 None:intergenic 25.0%
!! AGGTAAAATTGGTCTCTTTT+GGG + tig0026054:40316-40335 Msa1426610:five_prime_UTR 30.0%
AAAAAACCTTTAAGCTAGGT+AGG + tig0026054:40399-40418 Msa1426610:five_prime_UTR 30.0%
!!! TTGTAGACTTTAGTTTTGAG+GGG + tig0026054:40525-40544 Msa1426610:five_prime_UTR 30.0%
TTGATTCTCAGTGTAAACAA+AGG + tig0026054:40559-40578 Msa1426610:five_prime_UTR 30.0%
GAACGAGAGAAATATTGAAA+TGG - tig0026054:40738-40757 None:intergenic 30.0%
TTCAATATTTCTCTCGTTCT+CGG + tig0026054:40739-40758 Msa1426610:CDS 30.0%
CTGTTAAGTTCAAAATCCAA+AGG + tig0026054:40807-40826 Msa1426610:CDS 30.0%
GATACTCTACTTAAGGATTA+TGG + tig0026054:40899-40918 Msa1426610:CDS 30.0%
CATCAATGTCAAAACATCTT+CGG + tig0026054:40976-40995 Msa1426610:CDS 30.0%
ATCAATGTCAAAACATCTTC+GGG + tig0026054:40977-40996 Msa1426610:CDS 30.0%
ATGTAAAATACATCAAGCGA+AGG + tig0026054:41035-41054 Msa1426610:CDS 30.0%
TTCTTGTGGAAGAAAATTTG+AGG + tig0026054:41288-41307 Msa1426610:CDS 30.0%
TCCAAAAGAAAATCTCTATC+GGG - tig0026054:41367-41386 None:intergenic 30.0%
CTCCAAAAGAAAATCTCTAT+CGG - tig0026054:41368-41387 None:intergenic 30.0%
! CTGAAGCTAACTCAATTTTA+GGG - tig0026054:41405-41424 None:intergenic 30.0%
! ACTGAAGCTAACTCAATTTT+AGG - tig0026054:41406-41425 None:intergenic 30.0%
TACTTTATGCTTGATTCAAC+TGG + tig0026054:41511-41530 Msa1426610:CDS 30.0%
!! AAGTTATTTGGTGATGTGAA+GGG + tig0026054:41712-41731 Msa1426610:CDS 30.0%
CAGATGTTTGTGAAAAACAA+TGG + tig0026054:41826-41845 Msa1426610:CDS 30.0%
! TTTTGTGTACTTCATTTACC+GGG - tig0026054:41882-41901 None:intergenic 30.0%
AGTACACAAAATTAGTGTAC+TGG + tig0026054:41891-41910 Msa1426610:CDS 30.0%
TTGAAATAATGAAGTCTGGT+TGG + tig0026054:42328-42347 Msa1426610:intron 30.0%
!!! ATTGCACTTTTGAAGTTTCA+TGG + tig0026054:42532-42551 Msa1426610:CDS 30.0%
!!! ACTTTTGAAGTTTCATGGAT+GGG + tig0026054:42537-42556 Msa1426610:CDS 30.0%
TCAAGTTTCCAATTGCAAAA+GGG - tig0026054:42639-42658 None:intergenic 30.0%
ATCAAGTTTCCAATTGCAAA+AGG - tig0026054:42640-42659 None:intergenic 30.0%
GAGAGTTGAAAATCTTATCA+AGG - tig0026054:42789-42808 None:intergenic 30.0%
TTGATATCTGCAATAGTAAC+AGG + tig0026054:42823-42842 Msa1426610:three_prime_UTR 30.0%
!!! TTTTTAGTTCATAACAACGG+AGG + tig0026054:42890-42909 Msa1426610:three_prime_UTR 30.0%
! AAACTCAATGATGATGACGA+TGG - tig0026054:40159-40178 None:intergenic 35.0%
ATTAGTGCAAAAGAGGGTAA+CGG - tig0026054:40210-40229 None:intergenic 35.0%
TGAGCAATTAGTGCAAAAGA+GGG - tig0026054:40216-40235 None:intergenic 35.0%
TTGAGCAATTAGTGCAAAAG+AGG - tig0026054:40217-40236 None:intergenic 35.0%
!! GAGGTAAAATTGGTCTCTTT+TGG + tig0026054:40315-40334 Msa1426610:five_prime_UTR 35.0%
!! GGTAAAATTGGTCTCTTTTG+GGG + tig0026054:40317-40336 Msa1426610:five_prime_UTR 35.0%
TTAGTGAAAATGTCATCTGC+TGG + tig0026054:40620-40639 Msa1426610:exon 35.0%
AATGCATTTGGAGACAACAA+TGG - tig0026054:40680-40699 None:intergenic 35.0%
GATGGTAAATGGAATGCATT+TGG - tig0026054:40692-40711 None:intergenic 35.0%
AAGACAAGTGAGATGGTAAA+TGG - tig0026054:40703-40722 None:intergenic 35.0%
TCAATATTTCTCTCGTTCTC+GGG + tig0026054:40740-40759 Msa1426610:CDS 35.0%
! TCCTTTTCGTGTTATGGAAT+GGG + tig0026054:40772-40791 Msa1426610:CDS 35.0%
! AAACAGAAGTTGGTTTATGC+TGG + tig0026054:40857-40876 Msa1426610:CDS 35.0%
TCGAAGTGATACTCTACTTA+AGG + tig0026054:40892-40911 Msa1426610:CDS 35.0%
AAGGATTATGGAGTAACTGA+TGG + tig0026054:40911-40930 Msa1426610:CDS 35.0%
TCAATGTCAAAACATCTTCG+GGG + tig0026054:40978-40997 Msa1426610:CDS 35.0%
! GGGGAATGAATTTTCTTTTC+AGG + tig0026054:40997-41016 Msa1426610:CDS 35.0%
! GAAACAGTTTGATGATCTTG+AGG + tig0026054:41072-41091 Msa1426610:CDS 35.0%
TGCATCTGTTTGTTCGAAAA+AGG + tig0026054:41179-41198 Msa1426610:CDS 35.0%
GTTACAATGGACAACTCAAA+GGG - tig0026054:41226-41245 None:intergenic 35.0%
AGTTACAATGGACAACTCAA+AGG - tig0026054:41227-41246 None:intergenic 35.0%
! TGTGGAAGAAAATTTGAGGT+TGG + tig0026054:41292-41311 Msa1426610:CDS 35.0%
! TCCCGATAGAGATTTTCTTT+TGG + tig0026054:41363-41382 Msa1426610:CDS 35.0%
AGCTTCAGTGATTCGAAATA+TGG + tig0026054:41417-41436 Msa1426610:CDS 35.0%
!! TAATGATAATGGAAGACCTC+GGG - tig0026054:41596-41615 None:intergenic 35.0%
!! CTAATGATAATGGAAGACCT+CGG - tig0026054:41597-41616 None:intergenic 35.0%
GGTCTTCCATTATCATTAGA+CGG + tig0026054:41598-41617 Msa1426610:CDS 35.0%
!! GTTTTGGATCATCCAATAAG+TGG + tig0026054:41682-41701 Msa1426610:CDS 35.0%
!! CAAGTTATTTGGTGATGTGA+AGG + tig0026054:41711-41730 Msa1426610:CDS 35.0%
! GTTTTTCACAAACATCTGCA+TGG - tig0026054:41824-41843 None:intergenic 35.0%
TAGTGTACTGGACATAAGAT+TGG + tig0026054:41903-41922 Msa1426610:CDS 35.0%
TAAGATTGGCAAATGCAGAT+AGG + tig0026054:41917-41936 Msa1426610:CDS 35.0%
!!! GAATATTTTGATCAGCACAG+TGG + tig0026054:41948-41967 Msa1426610:CDS 35.0%
AAGCAGTATCCATGATCAAT+TGG - tig0026054:42000-42019 None:intergenic 35.0%
GTGTTGTCAATTGCAAATGA+TGG - tig0026054:42184-42203 None:intergenic 35.0%
ACATAGAAGAATTGCCACAT+CGG - tig0026054:42206-42225 None:intergenic 35.0%
AGCAACAACTGTTAAACGAT+TGG - tig0026054:42400-42419 None:intergenic 35.0%
!! GTACTTTTGAATGGCTTTAC+TGG + tig0026054:42440-42459 Msa1426610:CDS 35.0%
CCAATAGTTTCAGCAGAATA+CGG - tig0026054:42481-42500 None:intergenic 35.0%
CGTATTCTGCTGAAACTATT+GGG + tig0026054:42479-42498 Msa1426610:CDS 35.0%
TATTGGGTACATAAAGTCAC+TGG + tig0026054:42495-42514 Msa1426610:CDS 35.0%
!!! CACTTTTGAAGTTTCATGGA+TGG + tig0026054:42536-42555 Msa1426610:CDS 35.0%
!! TCAACCATGCTTTTGAAGAA+AGG + tig0026054:42595-42614 Msa1426610:CDS 35.0%
!! CAACCATGCTTTTGAAGAAA+GGG + tig0026054:42596-42615 Msa1426610:CDS 35.0%
!! AACCATGCTTTTGAAGAAAG+GGG + tig0026054:42597-42616 Msa1426610:CDS 35.0%
CAAGTTTCCAATTGCAAAAG+GGG - tig0026054:42638-42657 None:intergenic 35.0%
AATTGGAAACTTGATGTGCA+GGG + tig0026054:42645-42664 Msa1426610:CDS 35.0%
!! AGATGCTGTTTCTCAAATCA+TGG + tig0026054:42756-42775 Msa1426610:CDS 35.0%
!! AAGCGACACTCATTTTGAAA+CGG - tig0026054:43205-43224 None:intergenic 35.0%
!! TTCAAAATGAGTGTCGCTTT+AGG + tig0026054:42970-42989 Msa1426610:three_prime_UTR 35.0%
TTGGCACACAGATTAAGAAA+TGG + tig0026054:42995-43014 Msa1426610:three_prime_UTR 35.0%
TGTGAGAGAAAAGTTTGAGA+GGG + tig0026054:43105-43124 Msa1426610:three_prime_UTR 35.0%
AAAGTTTGAGAGGGTAAAGT+TGG + tig0026054:43114-43133 Msa1426610:three_prime_UTR 35.0%
TTAGTGCAAAAGAGGGTAAC+GGG - tig0026054:40209-40228 None:intergenic 40.0%
CATACTCCTACCTAGCTTAA+AGG - tig0026054:40408-40427 None:intergenic 40.0%
!! GGTTGAGCTGTTTGTTATGA+TGG + tig0026054:40488-40507 Msa1426610:five_prime_UTR 40.0%
TGTTAGTACTCTTAGTCCAG+TGG + tig0026054:40643-40662 Msa1426610:CDS 40.0%
! ACCATCTCACTTGTCTTTTG+AGG + tig0026054:40706-40725 Msa1426610:CDS 40.0%
TCCTCAAAAGACAAGTGAGA+TGG - tig0026054:40710-40729 None:intergenic 40.0%
!! GTTGACTCCTTTTCGTGTTA+TGG + tig0026054:40766-40785 Msa1426610:CDS 40.0%
! CTCCTTTTCGTGTTATGGAA+TGG + tig0026054:40771-40790 Msa1426610:CDS 40.0%
TCCCATTCCATAACACGAAA+AGG - tig0026054:40776-40795 None:intergenic 40.0%
!!! TTTTCTTTTCAGGTCGAACG+AGG + tig0026054:41007-41026 Msa1426610:CDS 40.0%
CAAGCGAAGGATTGCTAAAA+AGG + tig0026054:41048-41067 Msa1426610:CDS 40.0%
AGCAGGAACTTGTGTGTAAT+GGG + tig0026054:41095-41114 Msa1426610:CDS 40.0%
! AACTTGTGTGTAATGGGGAA+CGG + tig0026054:41101-41120 Msa1426610:CDS 40.0%
GTCCATTGTAACTACAGACT+TGG + tig0026054:41234-41253 Msa1426610:CDS 40.0%
AGCCAAGTCTGTAGTTACAA+TGG - tig0026054:41239-41258 None:intergenic 40.0%
! GGAAGAAAATTTGAGGTTGG+TGG + tig0026054:41295-41314 Msa1426610:CDS 40.0%
ATGGTAAACTCTGCATACGA+TGG + tig0026054:41436-41455 Msa1426610:CDS 40.0%
GCTAGCGAATACTATCCAAT+AGG + tig0026054:41463-41482 Msa1426610:CDS 40.0%
TATCCAATAGGATCTGCAGA+AGG + tig0026054:41475-41494 Msa1426610:CDS 40.0%
!! AATGATAATGGAAGACCTCG+GGG - tig0026054:41595-41614 None:intergenic 40.0%
CCTTCACCGTCTAATGATAA+TGG - tig0026054:41607-41626 None:intergenic 40.0%
CCATTATCATTAGACGGTGA+AGG + tig0026054:41604-41623 Msa1426610:CDS 40.0%
AGACGGTGAAGGCTTAAAAA+AGG + tig0026054:41615-41634 Msa1426610:CDS 40.0%
GACGGTGAAGGCTTAAAAAA+GGG + tig0026054:41616-41635 Msa1426610:CDS 40.0%
! AAGGGTACAATAGTTGGTCA+AGG + tig0026054:41634-41653 Msa1426610:CDS 40.0%
!! GGAAGTTGCTGCTTATGTTT+TGG + tig0026054:41666-41685 Msa1426610:CDS 40.0%
AAGCACTTGACCATCAATGT+TGG - tig0026054:41754-41773 None:intergenic 40.0%
ATGGTCAAGTGCTTGCATAA+GGG + tig0026054:41760-41779 Msa1426610:CDS 40.0%
CATAAGGGATTTAACCATCC+AGG + tig0026054:41775-41794 Msa1426610:CDS 40.0%
AATGGGAGCTGTGAAGATAT+CGG + tig0026054:41844-41863 Msa1426610:CDS 40.0%
!!! TATTTTGATCAGCACAGTGG+AGG + tig0026054:41951-41970 Msa1426610:CDS 40.0%
!! GTTCTGGTTCCAATTGATCA+TGG + tig0026054:41988-42007 Msa1426610:CDS 40.0%
CCGTATTCTGCTGAAACTAT+TGG + tig0026054:42478-42497 Msa1426610:CDS 40.0%
AGAGTTCGAGCACATTCAAT+TGG - tig0026054:42568-42587 None:intergenic 40.0%
AACCCCTTTCTTCAAAAGCA+TGG - tig0026054:42602-42621 None:intergenic 40.0%
CAATTGGAAACTTGATGTGC+AGG + tig0026054:42644-42663 Msa1426610:CDS 40.0%
GAACAAGGAATCTGTGATAG+AGG + tig0026054:42675-42694 Msa1426610:CDS 40.0%
TGAAAATCTTATCAAGGCGC+TGG - tig0026054:42783-42802 None:intergenic 40.0%
!! CAACGGAGGTTTGTTTTCTT+TGG + tig0026054:42904-42923 Msa1426610:three_prime_UTR 40.0%
!! GGAGGTTTGTTTTCTTTGGT+TGG + tig0026054:42908-42927 Msa1426610:three_prime_UTR 40.0%
!!! GACACTCATTTTGAAACGGA+GGG - tig0026054:42965-42984 None:intergenic 40.0%
GTGTGAGAGAAAAGTTTGAG+AGG + tig0026054:43104-43123 Msa1426610:three_prime_UTR 40.0%
AGCTTCCACCTTTCGATCTA+CGG + tig0026054:40239-40258 Msa1426610:five_prime_UTR 45.0%
AAACGACACCGTAGATCGAA+AGG - tig0026054:40250-40269 None:intergenic 45.0%
ATGGAAGACTCTCACACCTT+TGG - tig0026054:40826-40845 None:intergenic 45.0%
TATCACTTCGAGCGAGTTCA+CGG - tig0026054:40883-40902 None:intergenic 45.0%
!!! TTTTCAGGTCGAACGAGGTA+GGG + tig0026054:41012-41031 Msa1426610:CDS 45.0%
! GTTTGATGATCTTGAGGAGC+AGG + tig0026054:41078-41097 Msa1426610:CDS 45.0%
GAGCAGGAACTTGTGTGTAA+TGG + tig0026054:41094-41113 Msa1426610:CDS 45.0%
GCAGGAACTTGTGTGTAATG+GGG + tig0026054:41096-41115 Msa1426610:CDS 45.0%
CTGCAAGCATAATGATGCAG+TGG + tig0026054:41156-41175 Msa1426610:CDS 45.0%
TCTCTATCGGGAAGCTTTCT+TGG - tig0026054:41355-41374 None:intergenic 45.0%
GTACCTTCTGCAGATCCTAT+TGG - tig0026054:41481-41500 None:intergenic 45.0%
! ATAGGATCTGCAGAAGGTAC+CGG + tig0026054:41481-41500 Msa1426610:CDS 45.0%
AAGCATAAAGTAAGCTCCCC+CGG - tig0026054:41503-41522 None:intergenic 45.0%
!! ATGATAATGGAAGACCTCGG+GGG - tig0026054:41594-41613 None:intergenic 45.0%
AACTTGCGACGTCCACTTAT+TGG - tig0026054:41697-41716 None:intergenic 45.0%
AGTGGACGTCGCAAGTTATT+TGG + tig0026054:41700-41719 Msa1426610:CDS 45.0%
TGGTGTTCCTCCAACATTGA+TGG + tig0026054:41741-41760 Msa1426610:CDS 45.0%
CACTTGACCATCAATGTTGG+AGG - tig0026054:41751-41770 None:intergenic 45.0%
GATGGTCAAGTGCTTGCATA+AGG + tig0026054:41759-41778 Msa1426610:CDS 45.0%
TAACCATCCAGGAGACTTGA+TGG + tig0026054:41786-41805 Msa1426610:CDS 45.0%
ATCTTAGCCATCAAGTCTCC+TGG - tig0026054:41796-41815 None:intergenic 45.0%
! GGAGACTTGATGGCTAAGAT+TGG + tig0026054:41796-41815 Msa1426610:CDS 45.0%
CAAATGCAGATAGGCATGCT+GGG + tig0026054:41926-41945 Msa1426610:CDS 45.0%
CAATTGGAACCAGAACAGAC+TGG - tig0026054:41984-42003 None:intergenic 45.0%
TGCAATTGACAACACCGATG+TGG + tig0026054:42189-42208 Msa1426610:intron 45.0%
TAGTTTCAGCAGAATACGGC+TGG - tig0026054:42477-42496 None:intergenic 45.0%
TTGAAGAAAGGGGTTGAGAG+AGG + tig0026054:42607-42626 Msa1426610:CDS 45.0%
! GGATTGACCCCTTTTGCAAT+TGG + tig0026054:42628-42647 Msa1426610:CDS 45.0%
GTGCAGGGAATCATTGAACA+AGG + tig0026054:42660-42679 Msa1426610:CDS 45.0%
GCATTTGAGTCTGTTCTTCC+TGG + tig0026054:42712-42731 Msa1426610:CDS 45.0%
AATGTAGCTTCACTTGTGCC+AGG - tig0026054:42733-42752 None:intergenic 45.0%
!!! CGACACTCATTTTGAAACGG+AGG - tig0026054:42966-42985 None:intergenic 45.0%
!! ATGAGTGTCGCTTTAGGCTT+TGG + tig0026054:42976-42995 Msa1426610:three_prime_UTR 45.0%
GGAGACAACAATGGCTCAGT+GGG - tig0026054:40671-40690 None:intergenic 50.0%
TGGAGACAACAATGGCTCAG+TGG - tig0026054:40672-40691 None:intergenic 50.0%
!!! CTTTTCAGGTCGAACGAGGT+AGG + tig0026054:41011-41030 Msa1426610:CDS 50.0%
! TGTGTGTAATGGGGAACGGT+TGG + tig0026054:41105-41124 Msa1426610:CDS 50.0%
TACCGATGCCATTCAGAGAG+CGG + tig0026054:41327-41346 Msa1426610:CDS 50.0%
!! TAGGATCTGCAGAAGGTACC+GGG + tig0026054:41482-41501 Msa1426610:CDS 50.0%
!! TAGTTGGTCAAGGAGCGTTC+AGG + tig0026054:41644-41663 Msa1426610:CDS 50.0%
!! AGTTGGTCAAGGAGCGTTCA+GGG + tig0026054:41645-41664 Msa1426610:CDS 50.0%
! GGTGATGTGAAGGGTTTTGC+TGG + tig0026054:41721-41740 Msa1426610:CDS 50.0%
TAGCCATCAAGTCTCCTGGA+TGG - tig0026054:41792-41811 None:intergenic 50.0%
AGCTGTGAAGATATCGGTCC+TGG + tig0026054:41850-41869 Msa1426610:CDS 50.0%
GCTGTGAAGATATCGGTCCT+GGG + tig0026054:41851-41870 Msa1426610:CDS 50.0%
CTGTGAAGATATCGGTCCTG+GGG + tig0026054:41852-41871 Msa1426610:CDS 50.0%
GCAAATGCAGATAGGCATGC+TGG + tig0026054:41925-41944 Msa1426610:CDS 50.0%
GGACAATGACCAGTCTGTTC+TGG + tig0026054:41972-41991 Msa1426610:CDS 50.0%
CGACACCGTAGATCGAAAGG+TGG - tig0026054:40247-40266 None:intergenic 55.0%
AATGGCTCAGTGGGATCCAC+TGG - tig0026054:40662-40681 None:intergenic 55.0%
! GTGTAATGGGGAACGGTTGG+AGG + tig0026054:41108-41127 Msa1426610:CDS 55.0%
CACCGCTCTCTGAATGGCAT+CGG - tig0026054:41332-41351 None:intergenic 55.0%
TCTTGGCACCGCTCTCTGAA+TGG - tig0026054:41338-41357 None:intergenic 55.0%
!! AGGATCTGCAGAAGGTACCG+GGG + tig0026054:41483-41502 Msa1426610:CDS 55.0%
GCCTATCGACGAAGAGCCAA+TGG + tig0026054:41558-41577 Msa1426610:CDS 55.0%
!! GCCATTGGCTCTTCGTCGAT+AGG - tig0026054:41562-41581 None:intergenic 55.0%
CGGGGGTTATTCACAGCCAT+TGG - tig0026054:41577-41596 None:intergenic 55.0%
ATGGCTGTGAATAACCCCCG+AGG + tig0026054:41577-41596 Msa1426610:CDS 55.0%
TCATTTACCGGGAAAGCCCC+AGG - tig0026054:41871-41890 None:intergenic 55.0%
AGAATACGGCTGGCGAGCTT+GGG - tig0026054:42467-42486 None:intergenic 55.0%
!! GGATCTGCAGAAGGTACCGG+GGG + tig0026054:41484-41503 Msa1426610:CDS 60.0%
TATCGGTCCTGGGGCTTTCC+CGG + tig0026054:41861-41880 Msa1426610:CDS 60.0%
GAATACGGCTGGCGAGCTTG+GGG - tig0026054:42466-42485 None:intergenic 60.0%
CAGAATACGGCTGGCGAGCT+TGG - tig0026054:42468-42487 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0026054 gene 40145 43224 40145 ID=Msa1426610;Name=Msa1426610
tig0026054 mRNA 40145 43224 40145 ID=Msa1426610-mRNA-1;Parent=Msa1426610;Name=Msa1426610-mRNA-1;_AED=0.00;_eAED=0.00;_QI=484|1|1|1|1|1|2|408|595
tig0026054 exon 40145 42029 40145 ID=Msa1426610-mRNA-1:exon:5010;Parent=Msa1426610-mRNA-1
tig0026054 exon 42430 43224 42430 ID=Msa1426610-mRNA-1:exon:5011;Parent=Msa1426610-mRNA-1
tig0026054 five_prime_UTR 40145 40628 40145 ID=Msa1426610-mRNA-1:five_prime_utr;Parent=Msa1426610-mRNA-1
tig0026054 CDS 40629 42029 40629 ID=Msa1426610-mRNA-1:cds;Parent=Msa1426610-mRNA-1
tig0026054 CDS 42430 42816 42430 ID=Msa1426610-mRNA-1:cds;Parent=Msa1426610-mRNA-1
tig0026054 three_prime_UTR 42817 43224 42817 ID=Msa1426610-mRNA-1:three_prime_utr;Parent=Msa1426610-mRNA-1
Gene Sequence

>Msa1426610

ATGTCATCTGCTGGTGTTAGTACTCTTAGTCCAGTGGATCCCACTGAGCCATTGTTGTCTCCAAATGCATTCCATTTACCATCTCACTTGTCTTTTGAGGACAAATCCATTTCAATATTTCTCTCGTTCTCGGGTTCGTTGACTCCTTTTCGTGTTATGGAATGGGATACTATTGAGTCTGTTAAGTTCAAAATCCAAAGGTGTGAGAGTCTTCCATTTTTAACTAACAAACAGAAGTTGGTTTATGCTGGCCGTGAACTCGCTCGAAGTGATACTCTACTTAAGGATTATGGAGTAACTGATGGAAATGTTTTGCATTTGATAATCAAATTATCAGATCTTCAACTCATCAATGTCAAAACATCTTCGGGGAATGAATTTTCTTTTCAGGTCGAACGAGGTAGGGATGTAAAATACATCAAGCGAAGGATTGCTAAAAAGGAGAAACAGTTTGATGATCTTGAGGAGCAGGAACTTGTGTGTAATGGGGAACGGTTGGAGGATCAGAAGCTTATTGATGACATCTGCTGCAAGCATAATGATGCAGTGGTGCATCTGTTTGTTCGAAAAAGGAATGCAAAAGTTCATAGAAGACCCTTTGAGTTGTCCATTGTAACTACAGACTTGGCTGATACGAAAACTGACGATGTTTTTGAAAATTCTTGTGGAAGAAAATTTGAGGTTGGTGGTGTAGAAGATACCGATGCCATTCAGAGAGCGGTGCCAAGAAAGCTTCCCGATAGAGATTTTCTTTTGGAGCCAATTATAGTTAACCCTAAAATTGAGTTAGCTTCAGTGATTCGAAATATGGTAAACTCTGCATACGATGGACTAGCTAGCGAATACTATCCAATAGGATCTGCAGAAGGTACCGGGGGAGCTTACTTTATGCTTGATTCAACTGGTCAGAAGTATGTGTCTGTTTTTAAGCCTATCGACGAAGAGCCAATGGCTGTGAATAACCCCCGAGGTCTTCCATTATCATTAGACGGTGAAGGCTTAAAAAAGGGTACAATAGTTGGTCAAGGAGCGTTCAGGGAAGTTGCTGCTTATGTTTTGGATCATCCAATAAGTGGACGTCGCAAGTTATTTGGTGATGTGAAGGGTTTTGCTGGTGTTCCTCCAACATTGATGGTCAAGTGCTTGCATAAGGGATTTAACCATCCAGGAGACTTGATGGCTAAGATTGGATCCATGCAGATGTTTGTGAAAAACAATGGGAGCTGTGAAGATATCGGTCCTGGGGCTTTCCCGGTAAATGAAGTACACAAAATTAGTGTACTGGACATAAGATTGGCAAATGCAGATAGGCATGCTGGGAATATTTTGATCAGCACAGTGGAGGACAATGACCAGTCTGTTCTGGTTCCAATTGATCATGGATACTGCTTGCCCACAAGTTTTGAAGATTGTACTTTTGAATGGCTTTACTGGCCCCAAGCTCGCCAGCCGTATTCTGCTGAAACTATTGGGTACATAAAGTCACTGGATGCTGAAGAAGATATTGCACTTTTGAAGTTTCATGGATGGGATCTGCCAATTGAATGTGCTCGAACTCTTAGAATCTCAACCATGCTTTTGAAGAAAGGGGTTGAGAGAGGATTGACCCCTTTTGCAATTGGAAACTTGATGTGCAGGGAATCATTGAACAAGGAATCTGTGATAGAGGAAATAGTTGAAGAAGCATTTGAGTCTGTTCTTCCTGGCACAAGTGAAGCTACATTGCTAGATGCTGTTTCTCAAATCATGGACCAGCGCCTTGATAAGATTTTCAACTCTCTTTTTTAA
Protein sequence

>Msa1426610

MSSAGVSTLSPVDPTEPLLSPNAFHLPSHLSFEDKSISIFLSFSGSLTPFRVMEWDTIESVKFKIQRCESLPFLTNKQKLVYAGRELARSDTLLKDYGVTDGNVLHLIIKLSDLQLINVKTSSGNEFSFQVERGRDVKYIKRRIAKKEKQFDDLEEQELVCNGERLEDQKLIDDICCKHNDAVVHLFVRKRNAKVHRRPFELSIVTTDLADTKTDDVFENSCGRKFEVGGVEDTDAIQRAVPRKLPDRDFLLEPIIVNPKIELASVIRNMVNSAYDGLASEYYPIGSAEGTGGAYFMLDSTGQKYVSVFKPIDEEPMAVNNPRGLPLSLDGEGLKKGTIVGQGAFREVAAYVLDHPISGRRKLFGDVKGFAGVPPTLMVKCLHKGFNHPGDLMAKIGSMQMFVKNNGSCEDIGPGAFPVNEVHKISVLDIRLANADRHAGNILISTVEDNDQSVLVPIDHGYCLPTSFEDCTFEWLYWPQARQPYSAETIGYIKSLDAEEDIALLKFHGWDLPIECARTLRISTMLLKKGVERGLTPFAIGNLMCRESLNKESVIEEIVEEAFESVLPGTSEATLLDAVSQIMDQRLDKIFNSLF*