Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1434650 | XP_003631129.1 | 98.193 | 166 | 3 | 0 | 1 | 166 | 1 | 166 | 4.61e-118 | 340 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1434650 | G7LE73 | 98.193 | 166 | 3 | 0 | 1 | 166 | 1 | 166 | 2.20e-118 | 340 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa1354080 | Msa1434650 | 0.917092 | 8.088357e-86 | -8.615850e-47 |
| Msa1434650 | Msa1462610 | 0.813314 | 2.724805e-51 | -8.615850e-47 |
| Msa0792410 | Msa1434650 | 0.812683 | 3.746680e-51 | -8.615850e-47 |
| Msa1273320 | Msa1434650 | 0.810281 | 1.246354e-50 | -8.615850e-47 |
| Msa0307630 | Msa1434650 | 0.810311 | 1.227749e-50 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1434650 | MtrunA17_Chr8g0393161 | 98.193 | 166 | 3 | 0 | 1 | 166 | 1 | 166 | 4.24e-122 | 340 |
| Msa1434650 | MtrunA17_Chr1g0208621 | 33.708 | 178 | 98 | 4 | 1 | 165 | 1 | 171 | 2.43e-17 | 75.1 |
| Msa1434650 | MtrunA17_Chr5g0403161 | 30.303 | 99 | 68 | 1 | 1 | 98 | 1 | 99 | 4.21e-13 | 63.5 |
| Msa1434650 | MtrunA17_Chr1g0194831 | 26.857 | 175 | 100 | 5 | 1 | 161 | 1 | 161 | 2.58e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1434650 | AT1G18290.1 | 37.017 | 181 | 90 | 7 | 1 | 162 | 1 | 176 | 1.31e-30 | 109 |
| Msa1434650 | AT5G66580.1 | 34.524 | 84 | 55 | 0 | 15 | 98 | 16 | 99 | 5.98e-13 | 63.2 |
| Msa1434650 | AT4G37240.1 | 34.343 | 99 | 64 | 1 | 1 | 98 | 1 | 99 | 1.16e-12 | 62.8 |
| Msa1434650 | AT3G03280.1 | 30.539 | 167 | 105 | 4 | 1 | 161 | 1 | 162 | 2.33e-12 | 62.0 |
| Msa1434650 | AT2G23690.1 | 33.333 | 84 | 56 | 0 | 15 | 98 | 16 | 99 | 6.74e-11 | 58.2 |
Find 42 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATGCGAGTTTCAACAATT+TGG | 0.189626 | tig0027586:-2510 | Msa1434650:CDS |
| TCCAACCAAAGGGGTGTTTA+AGG | 0.257462 | tig0027586:-2786 | Msa1434650:CDS |
| TTTAAGGTGCTACTCTTAGA+TGG | 0.302251 | tig0027586:-2770 | Msa1434650:CDS |
| CATGCGAGTTTCAACAATTT+GGG | 0.302990 | tig0027586:-2509 | Msa1434650:CDS |
| GGGAGGCTGAACCTGTTAAA+AGG | 0.353755 | tig0027586:-2397 | Msa1434650:CDS |
| TAAGAGCACTCATTTCTTCA+TGG | 0.366168 | tig0027586:+2558 | None:intergenic |
| AGTTTCATTTATTGCCTTGA+TGG | 0.393034 | tig0027586:+2323 | None:intergenic |
| GGTACTCCAAGCAGAGATCT+TGG | 0.420093 | tig0027586:-2376 | Msa1434650:CDS |
| GAATTCGATGGCCAACTTTG+AGG | 0.420836 | tig0027586:+2663 | None:intergenic |
| AAAGTTGGCCATCGAATTCA+AGG | 0.441588 | tig0027586:-2659 | Msa1434650:CDS |
| CCTTGATGGAGTTTCATCAA+TGG | 0.445048 | tig0027586:+2337 | None:intergenic |
| TGAGTTCTGCATGTTCCCTT+CGG | 0.456879 | tig0027586:-2465 | Msa1434650:CDS |
| ACCTTAAACACCCCTTTGGT+TGG | 0.462577 | tig0027586:+2785 | None:intergenic |
| TAGCACCTTAAACACCCCTT+TGG | 0.471231 | tig0027586:+2781 | None:intergenic |
| CTGATAATTATGAGATGCTA+AGG | 0.473011 | tig0027586:-2418 | Msa1434650:CDS |
| AAGAGCACTCATTTCTTCAT+GGG | 0.480087 | tig0027586:+2559 | None:intergenic |
| CTTCTTGTACTCCAACCAAA+GGG | 0.491778 | tig0027586:-2796 | Msa1434650:CDS |
| GCGTGTTTCAAGTTCTTCAT+CGG | 0.493614 | tig0027586:+2628 | None:intergenic |
| TCATCAATGGTCTCCAATGC+AGG | 0.508093 | tig0027586:+2350 | None:intergenic |
| GAGTCACAAACAAACTGTCC+AGG | 0.528192 | tig0027586:+2692 | None:intergenic |
| ATTAAGAGCTGCAGAGCTGA+TGG | 0.532721 | tig0027586:-2723 | Msa1434650:CDS |
| GACTCAAGCCACCTCAAAGT+TGG | 0.535144 | tig0027586:-2674 | Msa1434650:CDS |
| GAGATCTTGGAGACCTGCAT+TGG | 0.537320 | tig0027586:-2363 | Msa1434650:CDS |
| GGGTTAACACAGAGTATAGC+AGG | 0.538805 | tig0027586:+2579 | None:intergenic |
| ATTGTTGAAACTCGCATGCT+TGG | 0.542497 | tig0027586:+2514 | None:intergenic |
| TTAAGGTGCTACTCTTAGAT+GGG | 0.544485 | tig0027586:-2769 | Msa1434650:CDS |
| GTTGAAACTCGCATGCTTGG+TGG | 0.552836 | tig0027586:+2517 | None:intergenic |
| ACAGAGTATAGCAGGTCCAT+TGG | 0.559102 | tig0027586:+2587 | None:intergenic |
| ACTCTTAGATGGGAGAATAG+AGG | 0.565120 | tig0027586:-2759 | Msa1434650:CDS |
| TTCGATGGCCAACTTTGAGG+TGG | 0.568058 | tig0027586:+2666 | None:intergenic |
| CTAATAGTCCTTGAATTCGA+TGG | 0.579984 | tig0027586:+2651 | None:intergenic |
| GTTCTGCATGTTCCCTTCGG+AGG | 0.580244 | tig0027586:-2462 | Msa1434650:CDS |
| CTCTTAACTACAAAGCGTCA+AGG | 0.588617 | tig0027586:-2541 | Msa1434650:CDS |
| GCTTCTTGTACTCCAACCAA+AGG | 0.589757 | tig0027586:-2797 | Msa1434650:CDS |
| TCTTAACTACAAAGCGTCAA+GGG | 0.619592 | tig0027586:-2540 | Msa1434650:CDS |
| GAGCTGATGGTAGAATACCC+TGG | 0.631828 | tig0027586:-2710 | Msa1434650:CDS |
| CCATTGATGAAACTCCATCA+AGG | 0.632248 | tig0027586:-2337 | Msa1434650:CDS |
| AGTCACAAACAAACTGTCCA+GGG | 0.643975 | tig0027586:+2693 | None:intergenic |
| TGATAATTATGAGATGCTAA+GGG | 0.645988 | tig0027586:-2417 | Msa1434650:CDS |
| AGGTCTCCAAGATCTCTGCT+TGG | 0.669783 | tig0027586:+2370 | None:intergenic |
| TAATTATGAGATGCTAAGGG+AGG | 0.689423 | tig0027586:-2414 | Msa1434650:CDS |
| TTCTTGTACTCCAACCAAAG+GGG | 0.691807 | tig0027586:-2795 | Msa1434650:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGATAATTATGAGATGCTAA+GGG | - | tig0027586:2723-2742 | Msa1434650:CDS | 25.0% |
| ! | GTTTTACTTTCTTCTACCAA+TGG | - | tig0027586:2537-2556 | Msa1434650:CDS | 30.0% |
| CTGATAATTATGAGATGCTA+AGG | - | tig0027586:2722-2741 | Msa1434650:CDS | 30.0% | |
| !! | TTTAAGGTGCTACTCTTAGA+TGG | - | tig0027586:2370-2389 | Msa1434650:CDS | 35.0% |
| !! | TTAAGGTGCTACTCTTAGAT+GGG | - | tig0027586:2371-2390 | Msa1434650:CDS | 35.0% |
| CTAATAGTCCTTGAATTCGA+TGG | + | tig0027586:2492-2511 | None:intergenic | 35.0% | |
| !! | AAGAGCACTCATTTCTTCAT+GGG | + | tig0027586:2584-2603 | None:intergenic | 35.0% |
| !! | TAAGAGCACTCATTTCTTCA+TGG | + | tig0027586:2585-2604 | None:intergenic | 35.0% |
| ! | TCTTAACTACAAAGCGTCAA+GGG | - | tig0027586:2600-2619 | Msa1434650:CDS | 35.0% |
| CATGCGAGTTTCAACAATTT+GGG | - | tig0027586:2631-2650 | Msa1434650:CDS | 35.0% | |
| ATGCAGAACTCACTAAAAAC+AGG | + | tig0027586:2667-2686 | None:intergenic | 35.0% | |
| TAATTATGAGATGCTAAGGG+AGG | - | tig0027586:2726-2745 | Msa1434650:CDS | 35.0% | |
| CTTCTTGTACTCCAACCAAA+GGG | - | tig0027586:2344-2363 | Msa1434650:CDS | 40.0% | |
| TTCTTGTACTCCAACCAAAG+GGG | - | tig0027586:2345-2364 | Msa1434650:CDS | 40.0% | |
| ACTCTTAGATGGGAGAATAG+AGG | - | tig0027586:2381-2400 | Msa1434650:CDS | 40.0% | |
| AGTCACAAACAAACTGTCCA+GGG | + | tig0027586:2450-2469 | None:intergenic | 40.0% | |
| AAAGTTGGCCATCGAATTCA+AGG | - | tig0027586:2481-2500 | Msa1434650:CDS | 40.0% | |
| !! | GCGTGTTTCAAGTTCTTCAT+CGG | + | tig0027586:2515-2534 | None:intergenic | 40.0% |
| ! | CTCTTAACTACAAAGCGTCA+AGG | - | tig0027586:2599-2618 | Msa1434650:CDS | 40.0% |
| ATTGTTGAAACTCGCATGCT+TGG | + | tig0027586:2629-2648 | None:intergenic | 40.0% | |
| GCATGCGAGTTTCAACAATT+TGG | - | tig0027586:2630-2649 | Msa1434650:CDS | 40.0% | |
| ! | CTATTTTCTTTGCCTCCGAA+GGG | + | tig0027586:2693-2712 | None:intergenic | 40.0% |
| ! | ACTATTTTCTTTGCCTCCGA+AGG | + | tig0027586:2694-2713 | None:intergenic | 40.0% |
| !! | TGCTTGGAGTACCTTTTAAC+AGG | + | tig0027586:2757-2776 | None:intergenic | 40.0% |
| CCTTGATGGAGTTTCATCAA+TGG | + | tig0027586:2806-2825 | None:intergenic | 40.0% | |
| CCATTGATGAAACTCCATCA+AGG | - | tig0027586:2803-2822 | Msa1434650:CDS | 40.0% | |
| GCTTCTTGTACTCCAACCAA+AGG | - | tig0027586:2343-2362 | Msa1434650:CDS | 45.0% | |
| !! | TCCAACCAAAGGGGTGTTTA+AGG | - | tig0027586:2354-2373 | Msa1434650:CDS | 45.0% |
| ACCTTAAACACCCCTTTGGT+TGG | + | tig0027586:2358-2377 | None:intergenic | 45.0% | |
| TAGCACCTTAAACACCCCTT+TGG | + | tig0027586:2362-2381 | None:intergenic | 45.0% | |
| ATTAAGAGCTGCAGAGCTGA+TGG | - | tig0027586:2417-2436 | Msa1434650:CDS | 45.0% | |
| GAGTCACAAACAAACTGTCC+AGG | + | tig0027586:2451-2470 | None:intergenic | 45.0% | |
| GAATTCGATGGCCAACTTTG+AGG | + | tig0027586:2480-2499 | None:intergenic | 45.0% | |
| ACAGAGTATAGCAGGTCCAT+TGG | + | tig0027586:2556-2575 | None:intergenic | 45.0% | |
| GGGTTAACACAGAGTATAGC+AGG | + | tig0027586:2564-2583 | None:intergenic | 45.0% | |
| TGAGTTCTGCATGTTCCCTT+CGG | - | tig0027586:2675-2694 | Msa1434650:CDS | 45.0% | |
| TCATCAATGGTCTCCAATGC+AGG | + | tig0027586:2793-2812 | None:intergenic | 45.0% | |
| GAGCTGATGGTAGAATACCC+TGG | - | tig0027586:2430-2449 | Msa1434650:CDS | 50.0% | |
| GACTCAAGCCACCTCAAAGT+TGG | - | tig0027586:2466-2485 | Msa1434650:CDS | 50.0% | |
| ! | TTCGATGGCCAACTTTGAGG+TGG | + | tig0027586:2477-2496 | None:intergenic | 50.0% |
| GTTGAAACTCGCATGCTTGG+TGG | + | tig0027586:2626-2645 | None:intergenic | 50.0% | |
| GGGAGGCTGAACCTGTTAAA+AGG | - | tig0027586:2743-2762 | Msa1434650:CDS | 50.0% | |
| GGTACTCCAAGCAGAGATCT+TGG | - | tig0027586:2764-2783 | Msa1434650:CDS | 50.0% | |
| AGGTCTCCAAGATCTCTGCT+TGG | + | tig0027586:2773-2792 | None:intergenic | 50.0% | |
| GAGATCTTGGAGACCTGCAT+TGG | - | tig0027586:2777-2796 | Msa1434650:CDS | 50.0% | |
| GTTCTGCATGTTCCCTTCGG+AGG | - | tig0027586:2678-2697 | Msa1434650:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0027586 | gene | 2331 | 2831 | 2331 | ID=Msa1434650;Name=Msa1434650 |
| tig0027586 | mRNA | 2331 | 2831 | 2331 | ID=Msa1434650-mRNA-1;Parent=Msa1434650;Name=Msa1434650-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|166 |
| tig0027586 | exon | 2331 | 2831 | 2331 | ID=Msa1434650-mRNA-1:exon:9304;Parent=Msa1434650-mRNA-1 |
| tig0027586 | CDS | 2331 | 2831 | 2331 | ID=Msa1434650-mRNA-1:cds;Parent=Msa1434650-mRNA-1 |
| Gene Sequence |
| Protein sequence |
