Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1354080 | XP_003631129.1 | 98.795 | 166 | 2 | 0 | 1 | 166 | 1 | 166 | 8.41e-119 | 342 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1354080 | G7LE73 | 98.795 | 166 | 2 | 0 | 1 | 166 | 1 | 166 | 4.02e-119 | 342 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0014590 | Msa1354080 | 0.802657 | 5.056129e-49 | -8.615850e-47 |
| Msa0017710 | Msa1354080 | 0.825489 | 4.560047e-54 | -8.615850e-47 |
| Msa0024650 | Msa1354080 | 0.801477 | 8.837926e-49 | -8.615850e-47 |
| Msa0073110 | Msa1354080 | 0.814199 | 1.740268e-51 | -8.615850e-47 |
| Msa0106540 | Msa1354080 | 0.807119 | 5.906949e-50 | -8.615850e-47 |
| Msa1354080 | Msa1380740 | 0.806481 | 8.056365e-50 | -8.615850e-47 |
| Msa1354080 | Msa1405830 | 0.804281 | 2.328786e-49 | -8.615850e-47 |
| Msa1354080 | Msa1434650 | 0.917092 | 8.088357e-86 | -8.615850e-47 |
| Msa1354080 | Msa1458270 | 0.809355 | 1.971803e-50 | -8.615850e-47 |
| Msa1354080 | Msa1461660 | 0.803274 | 3.769131e-49 | -8.615850e-47 |
| Msa1354080 | Msa1462610 | 0.829083 | 6.277517e-55 | -8.615850e-47 |
| Msa0649250 | Msa1354080 | -0.802150 | 6.429904e-49 | -8.615850e-47 |
| Msa0686830 | Msa1354080 | 0.800032 | 1.742881e-48 | -8.615850e-47 |
| Msa0792410 | Msa1354080 | 0.827602 | 1.429158e-54 | -8.615850e-47 |
| Msa0838210 | Msa1354080 | 0.811904 | 5.542421e-51 | -8.615850e-47 |
| Msa0121320 | Msa1354080 | 0.801589 | 8.383789e-49 | -8.615850e-47 |
| Msa1273320 | Msa1354080 | 0.809366 | 1.960572e-50 | -8.615850e-47 |
| Msa1295470 | Msa1354080 | -0.800843 | 1.191684e-48 | -8.615850e-47 |
| Msa1322500 | Msa1354080 | 0.809112 | 2.222481e-50 | -8.615850e-47 |
| Msa1323260 | Msa1354080 | -0.803596 | 3.232072e-49 | -8.615850e-47 |
| Msa0369990 | Msa1354080 | 0.801312 | 9.553973e-49 | -8.615850e-47 |
| Msa1002820 | Msa1354080 | 0.809924 | 1.487548e-50 | -8.615850e-47 |
| Msa1006030 | Msa1354080 | 0.806415 | 8.316764e-50 | -8.615850e-47 |
| Msa1029820 | Msa1354080 | 0.806365 | 8.524577e-50 | -8.615850e-47 |
| Msa1041910 | Msa1354080 | 0.812776 | 3.576671e-51 | -8.615850e-47 |
| Msa1048390 | Msa1354080 | 0.801919 | 7.174767e-49 | -8.615850e-47 |
| Msa1068730 | Msa1354080 | 0.800782 | 1.226496e-48 | -8.615850e-47 |
| Msa1068940 | Msa1354080 | 0.806954 | 6.401812e-50 | -8.615850e-47 |
| Msa1100330 | Msa1354080 | 0.801617 | 8.275857e-49 | -8.615850e-47 |
| Msa0279810 | Msa1354080 | 0.809977 | 1.448836e-50 | -8.615850e-47 |
| Msa0307630 | Msa1354080 | 0.826834 | 2.182696e-54 | -8.615850e-47 |
| Msa0311540 | Msa1354080 | 0.801208 | 1.003335e-48 | -8.615850e-47 |
| Msa0319220 | Msa1354080 | 0.810350 | 1.203979e-50 | -8.615850e-47 |
| Msa0354600 | Msa1354080 | 0.807317 | 5.362574e-50 | -8.615850e-47 |
| Msa1108560 | Msa1354080 | 0.815268 | 1.008641e-51 | -8.615850e-47 |
| Msa1167960 | Msa1354080 | 0.813075 | 3.075670e-51 | -8.615850e-47 |
| Msa1186340 | Msa1354080 | 0.809050 | 2.291522e-50 | -8.615850e-47 |
| Msa0575380 | Msa1354080 | 0.805433 | 1.338020e-49 | -8.615850e-47 |
| Msa0593870 | Msa1354080 | 0.802106 | 6.564985e-49 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1354080 | MtrunA17_Chr8g0393161 | 98.795 | 166 | 2 | 0 | 1 | 166 | 1 | 166 | 7.74e-123 | 342 |
| Msa1354080 | MtrunA17_Chr1g0208621 | 33.708 | 178 | 98 | 4 | 1 | 165 | 1 | 171 | 4.20e-17 | 74.3 |
| Msa1354080 | MtrunA17_Chr5g0403161 | 30.303 | 99 | 68 | 1 | 1 | 98 | 1 | 99 | 1.03e-12 | 62.4 |
| Msa1354080 | MtrunA17_Chr1g0194831 | 26.857 | 175 | 100 | 5 | 1 | 161 | 1 | 161 | 2.19e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1354080 | AT1G18290.1 | 37.017 | 181 | 90 | 7 | 1 | 162 | 1 | 176 | 4.18e-31 | 110 |
| Msa1354080 | AT5G66580.1 | 34.524 | 84 | 55 | 0 | 15 | 98 | 16 | 99 | 1.25e-12 | 62.4 |
| Msa1354080 | AT3G03280.1 | 30.539 | 167 | 105 | 4 | 1 | 161 | 1 | 162 | 2.78e-12 | 61.6 |
| Msa1354080 | AT4G37240.1 | 34.343 | 99 | 64 | 1 | 1 | 98 | 1 | 99 | 2.91e-12 | 61.6 |
| Msa1354080 | AT5G17350.1 | 29.193 | 161 | 98 | 4 | 15 | 161 | 21 | 179 | 9.35e-11 | 58.2 |
Find 41 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATGCGAGTTTCAACAATT+TGG | 0.189626 | tig0014199:-28461 | Msa1354080:CDS |
| TCCAACCAAAGGGGTGTTTA+AGG | 0.257462 | tig0014199:-28737 | Msa1354080:CDS |
| TTTAAGGTGCTACTCTTAGA+TGG | 0.302251 | tig0014199:-28721 | Msa1354080:CDS |
| CATGCGAGTTTCAACAATTT+GGG | 0.302990 | tig0014199:-28460 | Msa1354080:CDS |
| GGGAGGCTGAACCTGTTAAA+AGG | 0.353755 | tig0014199:-28348 | Msa1354080:CDS |
| TAAGAGCACTCATTTCTTCA+TGG | 0.366168 | tig0014199:+28509 | None:intergenic |
| AGTTTCATTTATTGCCTTGA+TGG | 0.393034 | tig0014199:+28274 | None:intergenic |
| GGTACTCCAAGCAGAGATCT+TGG | 0.420093 | tig0014199:-28327 | Msa1354080:CDS |
| AAAGTTGGCCATCGAATTCA+AGG | 0.441588 | tig0014199:-28610 | Msa1354080:CDS |
| CCTTGATGGAGTTTCATCAA+TGG | 0.445048 | tig0014199:+28288 | None:intergenic |
| GAATTCGATGGCCAACTTTG+AGG | 0.454460 | tig0014199:+28614 | None:intergenic |
| TGAGTTCTGCATGTTCCCTT+CGG | 0.456879 | tig0014199:-28416 | Msa1354080:CDS |
| ACCTTAAACACCCCTTTGGT+TGG | 0.462577 | tig0014199:+28736 | None:intergenic |
| TAGCACCTTAAACACCCCTT+TGG | 0.471231 | tig0014199:+28732 | None:intergenic |
| CTGATAATTATGAGATGCTA+AGG | 0.473011 | tig0014199:-28369 | Msa1354080:CDS |
| AAGAGCACTCATTTCTTCAT+GGG | 0.480087 | tig0014199:+28510 | None:intergenic |
| CTTCTTGTACTCCAACCAAA+GGG | 0.491778 | tig0014199:-28747 | Msa1354080:CDS |
| GCGTGTTTCAAGTTCTTCAT+CGG | 0.493614 | tig0014199:+28579 | None:intergenic |
| TCATCAATGGTCTCCAATGC+AGG | 0.508093 | tig0014199:+28301 | None:intergenic |
| GAGTCACAAACAAACTGTCC+AGG | 0.528192 | tig0014199:+28643 | None:intergenic |
| ATTAAGAGCTGCAGAGCTGA+TGG | 0.532721 | tig0014199:-28674 | Msa1354080:CDS |
| GACTCAAGCTACCTCAAAGT+TGG | 0.535872 | tig0014199:-28625 | Msa1354080:CDS |
| GAGATCTTGGAGACCTGCAT+TGG | 0.537320 | tig0014199:-28314 | Msa1354080:CDS |
| GGGTTAACACAGAGTATAGC+AGG | 0.538805 | tig0014199:+28530 | None:intergenic |
| ATTGTTGAAACTCGCATGCT+TGG | 0.542497 | tig0014199:+28465 | None:intergenic |
| TTAAGGTGCTACTCTTAGAT+GGG | 0.544485 | tig0014199:-28720 | Msa1354080:CDS |
| GTTGAAACTCGCATGCTTGG+TGG | 0.552836 | tig0014199:+28468 | None:intergenic |
| ACAGAGTATAGCAGGTCCAT+TGG | 0.559102 | tig0014199:+28538 | None:intergenic |
| ACTCTTAGATGGGAGAATAG+AGG | 0.565120 | tig0014199:-28710 | Msa1354080:CDS |
| CTAATAGTCCTTGAATTCGA+TGG | 0.579984 | tig0014199:+28602 | None:intergenic |
| GTTCTGCATGTTCCCTTCGG+AGG | 0.580244 | tig0014199:-28413 | Msa1354080:CDS |
| CTCTTAACTACAAAGCGTCA+AGG | 0.588617 | tig0014199:-28492 | Msa1354080:CDS |
| GCTTCTTGTACTCCAACCAA+AGG | 0.589757 | tig0014199:-28748 | Msa1354080:CDS |
| TCTTAACTACAAAGCGTCAA+GGG | 0.619592 | tig0014199:-28491 | Msa1354080:CDS |
| GAGCTGATGGTAGAATACCC+TGG | 0.631828 | tig0014199:-28661 | Msa1354080:CDS |
| CCATTGATGAAACTCCATCA+AGG | 0.632248 | tig0014199:-28288 | Msa1354080:CDS |
| AGTCACAAACAAACTGTCCA+GGG | 0.643975 | tig0014199:+28644 | None:intergenic |
| TGATAATTATGAGATGCTAA+GGG | 0.645988 | tig0014199:-28368 | Msa1354080:CDS |
| AGGTCTCCAAGATCTCTGCT+TGG | 0.669783 | tig0014199:+28321 | None:intergenic |
| TAATTATGAGATGCTAAGGG+AGG | 0.689423 | tig0014199:-28365 | Msa1354080:CDS |
| TTCTTGTACTCCAACCAAAG+GGG | 0.691807 | tig0014199:-28746 | Msa1354080:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TGATAATTATGAGATGCTAA+GGG | - | tig0014199:28674-28693 | Msa1354080:CDS | 25.0% |
| ! | GTTTTACTTTCTTCTACCAA+TGG | - | tig0014199:28488-28507 | Msa1354080:CDS | 30.0% |
| CTGATAATTATGAGATGCTA+AGG | - | tig0014199:28673-28692 | Msa1354080:CDS | 30.0% | |
| !! | TTTAAGGTGCTACTCTTAGA+TGG | - | tig0014199:28321-28340 | Msa1354080:CDS | 35.0% |
| !! | TTAAGGTGCTACTCTTAGAT+GGG | - | tig0014199:28322-28341 | Msa1354080:CDS | 35.0% |
| CTAATAGTCCTTGAATTCGA+TGG | + | tig0014199:28443-28462 | None:intergenic | 35.0% | |
| !! | AAGAGCACTCATTTCTTCAT+GGG | + | tig0014199:28535-28554 | None:intergenic | 35.0% |
| !! | TAAGAGCACTCATTTCTTCA+TGG | + | tig0014199:28536-28555 | None:intergenic | 35.0% |
| ! | TCTTAACTACAAAGCGTCAA+GGG | - | tig0014199:28551-28570 | Msa1354080:CDS | 35.0% |
| CATGCGAGTTTCAACAATTT+GGG | - | tig0014199:28582-28601 | Msa1354080:CDS | 35.0% | |
| ATGCAGAACTCACTAAAAAC+AGG | + | tig0014199:28618-28637 | None:intergenic | 35.0% | |
| TAATTATGAGATGCTAAGGG+AGG | - | tig0014199:28677-28696 | Msa1354080:CDS | 35.0% | |
| CTTCTTGTACTCCAACCAAA+GGG | - | tig0014199:28295-28314 | Msa1354080:CDS | 40.0% | |
| TTCTTGTACTCCAACCAAAG+GGG | - | tig0014199:28296-28315 | Msa1354080:CDS | 40.0% | |
| ACTCTTAGATGGGAGAATAG+AGG | - | tig0014199:28332-28351 | Msa1354080:CDS | 40.0% | |
| AGTCACAAACAAACTGTCCA+GGG | + | tig0014199:28401-28420 | None:intergenic | 40.0% | |
| AAAGTTGGCCATCGAATTCA+AGG | - | tig0014199:28432-28451 | Msa1354080:CDS | 40.0% | |
| !! | GCGTGTTTCAAGTTCTTCAT+CGG | + | tig0014199:28466-28485 | None:intergenic | 40.0% |
| ! | CTCTTAACTACAAAGCGTCA+AGG | - | tig0014199:28550-28569 | Msa1354080:CDS | 40.0% |
| ATTGTTGAAACTCGCATGCT+TGG | + | tig0014199:28580-28599 | None:intergenic | 40.0% | |
| GCATGCGAGTTTCAACAATT+TGG | - | tig0014199:28581-28600 | Msa1354080:CDS | 40.0% | |
| ! | CTATTTTCTTTGCCTCCGAA+GGG | + | tig0014199:28644-28663 | None:intergenic | 40.0% |
| ! | ACTATTTTCTTTGCCTCCGA+AGG | + | tig0014199:28645-28664 | None:intergenic | 40.0% |
| !! | TGCTTGGAGTACCTTTTAAC+AGG | + | tig0014199:28708-28727 | None:intergenic | 40.0% |
| CCTTGATGGAGTTTCATCAA+TGG | + | tig0014199:28757-28776 | None:intergenic | 40.0% | |
| CCATTGATGAAACTCCATCA+AGG | - | tig0014199:28754-28773 | Msa1354080:CDS | 40.0% | |
| GCTTCTTGTACTCCAACCAA+AGG | - | tig0014199:28294-28313 | Msa1354080:CDS | 45.0% | |
| !! | TCCAACCAAAGGGGTGTTTA+AGG | - | tig0014199:28305-28324 | Msa1354080:CDS | 45.0% |
| ACCTTAAACACCCCTTTGGT+TGG | + | tig0014199:28309-28328 | None:intergenic | 45.0% | |
| TAGCACCTTAAACACCCCTT+TGG | + | tig0014199:28313-28332 | None:intergenic | 45.0% | |
| ATTAAGAGCTGCAGAGCTGA+TGG | - | tig0014199:28368-28387 | Msa1354080:CDS | 45.0% | |
| GAGTCACAAACAAACTGTCC+AGG | + | tig0014199:28402-28421 | None:intergenic | 45.0% | |
| GACTCAAGCTACCTCAAAGT+TGG | - | tig0014199:28417-28436 | Msa1354080:CDS | 45.0% | |
| GAATTCGATGGCCAACTTTG+AGG | + | tig0014199:28431-28450 | None:intergenic | 45.0% | |
| ACAGAGTATAGCAGGTCCAT+TGG | + | tig0014199:28507-28526 | None:intergenic | 45.0% | |
| GGGTTAACACAGAGTATAGC+AGG | + | tig0014199:28515-28534 | None:intergenic | 45.0% | |
| TGAGTTCTGCATGTTCCCTT+CGG | - | tig0014199:28626-28645 | Msa1354080:CDS | 45.0% | |
| TCATCAATGGTCTCCAATGC+AGG | + | tig0014199:28744-28763 | None:intergenic | 45.0% | |
| GAGCTGATGGTAGAATACCC+TGG | - | tig0014199:28381-28400 | Msa1354080:CDS | 50.0% | |
| GTTGAAACTCGCATGCTTGG+TGG | + | tig0014199:28577-28596 | None:intergenic | 50.0% | |
| GGGAGGCTGAACCTGTTAAA+AGG | - | tig0014199:28694-28713 | Msa1354080:CDS | 50.0% | |
| GGTACTCCAAGCAGAGATCT+TGG | - | tig0014199:28715-28734 | Msa1354080:CDS | 50.0% | |
| AGGTCTCCAAGATCTCTGCT+TGG | + | tig0014199:28724-28743 | None:intergenic | 50.0% | |
| GAGATCTTGGAGACCTGCAT+TGG | - | tig0014199:28728-28747 | Msa1354080:CDS | 50.0% | |
| GTTCTGCATGTTCCCTTCGG+AGG | - | tig0014199:28629-28648 | Msa1354080:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0014199 | gene | 28282 | 28782 | 28282 | ID=Msa1354080;Name=Msa1354080 |
| tig0014199 | mRNA | 28282 | 28782 | 28282 | ID=Msa1354080-mRNA-1;Parent=Msa1354080;Name=Msa1354080-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|166 |
| tig0014199 | exon | 28282 | 28782 | 28282 | ID=Msa1354080-mRNA-1:exon:8943;Parent=Msa1354080-mRNA-1 |
| tig0014199 | CDS | 28282 | 28782 | 28282 | ID=Msa1354080-mRNA-1:cds;Parent=Msa1354080-mRNA-1 |
| Gene Sequence |
| Protein sequence |
