AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1437510


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1437510 MtrunA17_Chr4g0069271 100.000 105 0 0 1 105 165 269 2.69e-72 216
Msa1437510 MtrunA17_Chr2g0302561 96.040 101 4 0 1 101 165 265 2.26e-66 200
Msa1437510 MtrunA17_Chr4g0055321 76.768 99 21 1 1 97 162 260 2.02e-42 139
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1437510 AT1G48240.2 86.869 99 13 0 1 99 115 213 3.45e-53 166
Msa1437510 AT1G48240.1 86.869 99 13 0 1 99 166 264 8.26e-53 166
Msa1437510 AT3G17440.1 85.000 100 15 0 1 100 166 265 2.30e-52 165
Msa1437510 AT2G35190.1 79.798 99 18 1 1 97 164 262 1.76e-41 137
Msa1437510 AT3G17440.2 85.106 47 7 0 1 47 166 212 1.73e-21 84.7

Find 39 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATATTAGAGGATTGCTTTC+TGG 0.171816 tig0028307:-54476 Msa1437510:three_prime_UTR
ATCCCAACAACAAAGATATT+AGG 0.293546 tig0028307:-54641 Msa1437510:CDS
CCTTCAAGGTAGAAGCAGTT+TGG 0.343816 tig0028307:+55335 None:intergenic
TCCCAACAACAAAGATATTA+GGG 0.359712 tig0028307:-54640 Msa1437510:CDS
CTTCAAGGTAGAAGCAGTTT+GGG 0.360070 tig0028307:+55336 None:intergenic
ATCCCTAATATCTTTGTTGT+TGG 0.373848 tig0028307:+54638 None:intergenic
CAAACTTACTTGGCCCTTCA+AGG 0.377735 tig0028307:+55321 None:intergenic
TCTTATCAATCGAATATTAG+AGG 0.380556 tig0028307:-54488 Msa1437510:three_prime_UTR
CCAAACTGCTTCTACCTTGA+AGG 0.383146 tig0028307:-55335 Msa1437510:CDS
TCCCTAATATCTTTGTTGTT+GGG 0.383563 tig0028307:+54639 None:intergenic
TCCCAACTTGTTAAGGAGAT+TGG 0.395927 tig0028307:-55154 Msa1437510:CDS
TCATACCTTCACGACAATAA+TGG 0.396104 tig0028307:+54980 None:intergenic
AAAAGCCTCCTTGAGGGAAC+TGG 0.407527 tig0028307:+54597 None:intergenic
GAAGGCCTCCCAACTTGTTA+AGG 0.410294 tig0028307:-55161 Msa1437510:CDS
AAAGATATTAGGGATATTCC+AGG 0.415143 tig0028307:-54630 Msa1437510:CDS
CAATTCTAGTCCTGCCAACT+TGG 0.415915 tig0028307:-54518 Msa1437510:three_prime_UTR
AATCATGCAGACTGAACAAA+TGG 0.422006 tig0028307:-55227 Msa1437510:intron
GGAACTGGAGGCGCCAATCC+TGG 0.433221 tig0028307:+54612 None:intergenic
TGTTAAGGAGATTGGTAGAC+AGG 0.437254 tig0028307:-55146 Msa1437510:intron
ACCAATCTCCTTAACAAGTT+GGG 0.449301 tig0028307:+55153 None:intergenic
TACCAATCTCCTTAACAAGT+TGG 0.487013 tig0028307:+55152 None:intergenic
TATTAGGGATATTCCAGGAT+TGG 0.491096 tig0028307:-54625 Msa1437510:CDS
GTACATCAAACAATTGAAGT+TGG 0.510767 tig0028307:-55361 None:intergenic
AATCAAGCTCATTAACAATA+CGG 0.513503 tig0028307:+55203 None:intergenic
ACGTACAAAAGCCTCCTTGA+GGG 0.543026 tig0028307:+54591 None:intergenic
TTTCTGTTTCTTATCGTCTG+TGG 0.544072 tig0028307:-55011 Msa1437510:CDS
AGAACAAGAACCAAGTTGGC+AGG 0.550428 tig0028307:+54508 None:intergenic
GATAAGAACAAGAACCAAGT+TGG 0.555394 tig0028307:+54504 None:intergenic
CAAACTGCTTCTACCTTGAA+GGG 0.570444 tig0028307:-55334 Msa1437510:CDS
ATTAGAGGATTGCTTTCTGG+AGG 0.581140 tig0028307:-54473 Msa1437510:three_prime_UTR
TGGCGCCTCCAGTTCCCTCA+AGG 0.584416 tig0028307:-54605 Msa1437510:CDS
CGCCTCCAGTTCCCTCAAGG+AGG 0.588015 tig0028307:-54602 Msa1437510:CDS
ATTAAGAGACGTGGCACTCG+AGG 0.588508 tig0028307:+54351 None:intergenic
AGCCTCCTTGAGGGAACTGG+AGG 0.604127 tig0028307:+54600 None:intergenic
GATTGCCATTATTGTCGTGA+AGG 0.622761 tig0028307:-54985 Msa1437510:intron
ATCATGCAGACTGAACAAAT+GGG 0.629726 tig0028307:-55226 Msa1437510:intron
AATCTCCTTAACAAGTTGGG+AGG 0.641193 tig0028307:+55156 None:intergenic
GATTGTTACATTAAGAGACG+TGG 0.682955 tig0028307:+54342 None:intergenic
TACGTACAAAAGCCTCCTTG+AGG 0.702572 tig0028307:+54590 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATTATTAGAGCAAAT+TGG + tig0028307:54802-54821 None:intergenic 15.0%
!!! TCAACTATTTTAAATAACTT+TGG - tig0028307:54840-54859 Msa1437510:intron 15.0%
!! ATAAAATTATTAGAGCAAAT+TGG + tig0028307:54802-54821 None:intergenic 15.0%
!!! TCAACTATTTTAAATAACTT+TGG - tig0028307:54840-54859 Msa1437510:intron 15.0%
!!! AATTCAGTTTTCAATAAAGA+AGG - tig0028307:54518-54537 Msa1437510:three_prime_UTR 20.0%
!!! CTTTAATTTCCTTGTTTATA+TGG - tig0028307:54577-54596 Msa1437510:CDS 20.0%
!! AGAGAATATATCAACAAATT+AGG + tig0028307:54766-54785 None:intergenic 20.0%
!! TGAAAAAAGAAAGAATTAAC+TGG + tig0028307:55266-55285 None:intergenic 20.0%
!!! AATTCAGTTTTCAATAAAGA+AGG - tig0028307:54518-54537 Msa1437510:three_prime_UTR 20.0%
!!! CTTTAATTTCCTTGTTTATA+TGG - tig0028307:54577-54596 Msa1437510:CDS 20.0%
!! AGAGAATATATCAACAAATT+AGG + tig0028307:54766-54785 None:intergenic 20.0%
!! TGAAAAAAGAAAGAATTAAC+TGG + tig0028307:55266-55285 None:intergenic 20.0%
! CAATTCAAATCAAACTTACT+TGG + tig0028307:54389-54408 None:intergenic 25.0%
! AATCAAGCTCATTAACAATA+CGG + tig0028307:54497-54516 None:intergenic 25.0%
! ACATTATTCTACTTGAGAAA+AGG - tig0028307:54867-54886 Msa1437510:intron 25.0%
! TCTTATCAATCGAATATTAG+AGG - tig0028307:55209-55228 Msa1437510:CDS 25.0%
! CAATTCAAATCAAACTTACT+TGG + tig0028307:54389-54408 None:intergenic 25.0%
! AATCAAGCTCATTAACAATA+CGG + tig0028307:54497-54516 None:intergenic 25.0%
! ACATTATTCTACTTGAGAAA+AGG - tig0028307:54867-54886 Msa1437510:intron 25.0%
! TCTTATCAATCGAATATTAG+AGG - tig0028307:55209-55228 Msa1437510:CDS 25.0%
GAATTGAGTATGCTATAAGA+AGG - tig0028307:54403-54422 Msa1437510:three_prime_UTR 30.0%
ATCCCAACAACAAAGATATT+AGG - tig0028307:55056-55075 Msa1437510:intron 30.0%
TCCCAACAACAAAGATATTA+GGG - tig0028307:55057-55076 Msa1437510:intron 30.0%
!! TCCCTAATATCTTTGTTGTT+GGG + tig0028307:55061-55080 None:intergenic 30.0%
! ATCCCTAATATCTTTGTTGT+TGG + tig0028307:55062-55081 None:intergenic 30.0%
AAAGATATTAGGGATATTCC+AGG - tig0028307:55067-55086 Msa1437510:intron 30.0%
AATATTAGAGGATTGCTTTC+TGG - tig0028307:55221-55240 Msa1437510:intron 30.0%
GAATTGAGTATGCTATAAGA+AGG - tig0028307:54403-54422 Msa1437510:three_prime_UTR 30.0%
ATCCCAACAACAAAGATATT+AGG - tig0028307:55056-55075 Msa1437510:intron 30.0%
TCCCAACAACAAAGATATTA+GGG - tig0028307:55057-55076 Msa1437510:intron 30.0%
!! TCCCTAATATCTTTGTTGTT+GGG + tig0028307:55061-55080 None:intergenic 30.0%
! ATCCCTAATATCTTTGTTGT+TGG + tig0028307:55062-55081 None:intergenic 30.0%
AAAGATATTAGGGATATTCC+AGG - tig0028307:55067-55086 Msa1437510:intron 30.0%
AATATTAGAGGATTGCTTTC+TGG - tig0028307:55221-55240 Msa1437510:intron 30.0%
CTATAAGAAGGAAAGAGTCT+AGG - tig0028307:54415-54434 Msa1437510:three_prime_UTR 35.0%
AATCATGCAGACTGAACAAA+TGG - tig0028307:54470-54489 Msa1437510:three_prime_UTR 35.0%
ATCATGCAGACTGAACAAAT+GGG - tig0028307:54471-54490 Msa1437510:three_prime_UTR 35.0%
ACCAATCTCCTTAACAAGTT+GGG + tig0028307:54547-54566 None:intergenic 35.0%
TACCAATCTCCTTAACAAGT+TGG + tig0028307:54548-54567 None:intergenic 35.0%
TACAGCAGTCCATATAAACA+AGG + tig0028307:54589-54608 None:intergenic 35.0%
TATTTCTTCCAAACCTGTCA+TGG - tig0028307:54607-54626 Msa1437510:CDS 35.0%
! TTTCTGTTTCTTATCGTCTG+TGG - tig0028307:54686-54705 Msa1437510:intron 35.0%
TCATACCTTCACGACAATAA+TGG + tig0028307:54720-54739 None:intergenic 35.0%
TGTGCACATAAACATGCAAT+AGG + tig0028307:54941-54960 None:intergenic 35.0%
TATTAGGGATATTCCAGGAT+TGG - tig0028307:55072-55091 Msa1437510:intron 35.0%
GATAAGAACAAGAACCAAGT+TGG + tig0028307:55196-55215 None:intergenic 35.0%
CTATAAGAAGGAAAGAGTCT+AGG - tig0028307:54415-54434 Msa1437510:three_prime_UTR 35.0%
AATCATGCAGACTGAACAAA+TGG - tig0028307:54470-54489 Msa1437510:three_prime_UTR 35.0%
ATCATGCAGACTGAACAAAT+GGG - tig0028307:54471-54490 Msa1437510:three_prime_UTR 35.0%
ACCAATCTCCTTAACAAGTT+GGG + tig0028307:54547-54566 None:intergenic 35.0%
TACCAATCTCCTTAACAAGT+TGG + tig0028307:54548-54567 None:intergenic 35.0%
TACAGCAGTCCATATAAACA+AGG + tig0028307:54589-54608 None:intergenic 35.0%
TATTTCTTCCAAACCTGTCA+TGG - tig0028307:54607-54626 Msa1437510:CDS 35.0%
! TTTCTGTTTCTTATCGTCTG+TGG - tig0028307:54686-54705 Msa1437510:intron 35.0%
TCATACCTTCACGACAATAA+TGG + tig0028307:54720-54739 None:intergenic 35.0%
TGTGCACATAAACATGCAAT+AGG + tig0028307:54941-54960 None:intergenic 35.0%
TATTAGGGATATTCCAGGAT+TGG - tig0028307:55072-55091 Msa1437510:intron 35.0%
GATAAGAACAAGAACCAAGT+TGG + tig0028307:55196-55215 None:intergenic 35.0%
CTTCAAGGTAGAAGCAGTTT+GGG + tig0028307:54364-54383 None:intergenic 40.0%
CAAACTGCTTCTACCTTGAA+GGG - tig0028307:54363-54382 Msa1437510:three_prime_UTR 40.0%
! AATCTCCTTAACAAGTTGGG+AGG + tig0028307:54544-54563 None:intergenic 40.0%
!! TCCCAACTTGTTAAGGAGAT+TGG - tig0028307:54543-54562 Msa1437510:three_prime_UTR 40.0%
TGTTAAGGAGATTGGTAGAC+AGG - tig0028307:54551-54570 Msa1437510:exon 40.0%
ACTTAGTTCCATGACAGGTT+TGG + tig0028307:54618-54637 None:intergenic 40.0%
TCTGTAGCAACCTGATAACA+GGG + tig0028307:54650-54669 None:intergenic 40.0%
GATTGCCATTATTGTCGTGA+AGG - tig0028307:54712-54731 Msa1437510:intron 40.0%
GCAATCACTATTCACTAGCA+CGG + tig0028307:54919-54938 None:intergenic 40.0%
ATTAGAGGATTGCTTTCTGG+AGG - tig0028307:55224-55243 Msa1437510:intron 40.0%
CTTCAAGGTAGAAGCAGTTT+GGG + tig0028307:54364-54383 None:intergenic 40.0%
CAAACTGCTTCTACCTTGAA+GGG - tig0028307:54363-54382 Msa1437510:three_prime_UTR 40.0%
! AATCTCCTTAACAAGTTGGG+AGG + tig0028307:54544-54563 None:intergenic 40.0%
!! TCCCAACTTGTTAAGGAGAT+TGG - tig0028307:54543-54562 Msa1437510:three_prime_UTR 40.0%
TGTTAAGGAGATTGGTAGAC+AGG - tig0028307:54551-54570 Msa1437510:exon 40.0%
ACTTAGTTCCATGACAGGTT+TGG + tig0028307:54618-54637 None:intergenic 40.0%
TCTGTAGCAACCTGATAACA+GGG + tig0028307:54650-54669 None:intergenic 40.0%
GATTGCCATTATTGTCGTGA+AGG - tig0028307:54712-54731 Msa1437510:intron 40.0%
GCAATCACTATTCACTAGCA+CGG + tig0028307:54919-54938 None:intergenic 40.0%
ATTAGAGGATTGCTTTCTGG+AGG - tig0028307:55224-55243 Msa1437510:intron 40.0%
CCTTCAAGGTAGAAGCAGTT+TGG + tig0028307:54365-54384 None:intergenic 45.0%
CCAAACTGCTTCTACCTTGA+AGG - tig0028307:54362-54381 Msa1437510:three_prime_UTR 45.0%
CAAACTTACTTGGCCCTTCA+AGG + tig0028307:54379-54398 None:intergenic 45.0%
GGACAACTTAGTTCCATGAC+AGG + tig0028307:54623-54642 None:intergenic 45.0%
TTGTCCTTACCCCTGTTATC+AGG - tig0028307:54637-54656 Msa1437510:CDS 45.0%
CTGTAGCAACCTGATAACAG+GGG + tig0028307:54649-54668 None:intergenic 45.0%
GTCTGTAGCAACCTGATAAC+AGG + tig0028307:54651-54670 None:intergenic 45.0%
! TGAGAAAAGGTGCGAGAACT+TGG - tig0028307:54880-54899 Msa1437510:intron 45.0%
GTGCGAGAACTTGGATTTCA+TGG - tig0028307:54889-54908 Msa1437510:intron 45.0%
ACGTACAAAAGCCTCCTTGA+GGG + tig0028307:55109-55128 None:intergenic 45.0%
TACGTACAAAAGCCTCCTTG+AGG + tig0028307:55110-55129 None:intergenic 45.0%
! CAAGGAGGCTTTTGTACGTA+CGG - tig0028307:55110-55129 Msa1437510:intron 45.0%
CAATTCTAGTCCTGCCAACT+TGG - tig0028307:55179-55198 Msa1437510:CDS 45.0%
! AGAACAAGAACCAAGTTGGC+AGG + tig0028307:55192-55211 None:intergenic 45.0%
CCTTCAAGGTAGAAGCAGTT+TGG + tig0028307:54365-54384 None:intergenic 45.0%
CCAAACTGCTTCTACCTTGA+AGG - tig0028307:54362-54381 Msa1437510:three_prime_UTR 45.0%
CAAACTTACTTGGCCCTTCA+AGG + tig0028307:54379-54398 None:intergenic 45.0%
GGACAACTTAGTTCCATGAC+AGG + tig0028307:54623-54642 None:intergenic 45.0%
TTGTCCTTACCCCTGTTATC+AGG - tig0028307:54637-54656 Msa1437510:CDS 45.0%
CTGTAGCAACCTGATAACAG+GGG + tig0028307:54649-54668 None:intergenic 45.0%
GTCTGTAGCAACCTGATAAC+AGG + tig0028307:54651-54670 None:intergenic 45.0%
! TGAGAAAAGGTGCGAGAACT+TGG - tig0028307:54880-54899 Msa1437510:intron 45.0%
GTGCGAGAACTTGGATTTCA+TGG - tig0028307:54889-54908 Msa1437510:intron 45.0%
ACGTACAAAAGCCTCCTTGA+GGG + tig0028307:55109-55128 None:intergenic 45.0%
TACGTACAAAAGCCTCCTTG+AGG + tig0028307:55110-55129 None:intergenic 45.0%
! CAAGGAGGCTTTTGTACGTA+CGG - tig0028307:55110-55129 Msa1437510:intron 45.0%
CAATTCTAGTCCTGCCAACT+TGG - tig0028307:55179-55198 Msa1437510:CDS 45.0%
! AGAACAAGAACCAAGTTGGC+AGG + tig0028307:55192-55211 None:intergenic 45.0%
!! GAAGGCCTCCCAACTTGTTA+AGG - tig0028307:54536-54555 Msa1437510:three_prime_UTR 50.0%
GCAACCTGATAACAGGGGTA+AGG + tig0028307:54644-54663 None:intergenic 50.0%
AAAAGCCTCCTTGAGGGAAC+TGG + tig0028307:55103-55122 None:intergenic 50.0%
! AGGCTTTTGTACGTACGGAC+TGG - tig0028307:55115-55134 Msa1437510:intron 50.0%
ATTAAGAGACGTGGCACTCG+AGG + tig0028307:55349-55368 None:intergenic 50.0%
!! GAAGGCCTCCCAACTTGTTA+AGG - tig0028307:54536-54555 Msa1437510:three_prime_UTR 50.0%
GCAACCTGATAACAGGGGTA+AGG + tig0028307:54644-54663 None:intergenic 50.0%
AAAAGCCTCCTTGAGGGAAC+TGG + tig0028307:55103-55122 None:intergenic 50.0%
! AGGCTTTTGTACGTACGGAC+TGG - tig0028307:55115-55134 Msa1437510:intron 50.0%
ATTAAGAGACGTGGCACTCG+AGG + tig0028307:55349-55368 None:intergenic 50.0%
AGCCTCCTTGAGGGAACTGG+AGG + tig0028307:55100-55119 None:intergenic 60.0%
AGCCTCCTTGAGGGAACTGG+AGG + tig0028307:55100-55119 None:intergenic 60.0%
!! GGAACTGGAGGCGCCAATCC+TGG + tig0028307:55088-55107 None:intergenic 65.0%
TGGCGCCTCCAGTTCCCTCA+AGG - tig0028307:55092-55111 Msa1437510:intron 65.0%
CGCCTCCAGTTCCCTCAAGG+AGG - tig0028307:55095-55114 Msa1437510:intron 65.0%
!! GGAACTGGAGGCGCCAATCC+TGG + tig0028307:55088-55107 None:intergenic 65.0%
TGGCGCCTCCAGTTCCCTCA+AGG - tig0028307:55092-55111 Msa1437510:intron 65.0%
CGCCTCCAGTTCCCTCAAGG+AGG - tig0028307:55095-55114 Msa1437510:intron 65.0%
Chromosome Type Strat End Strand Name
tig0028307 gene 54345 55374 54345 ID=Msa1437510;Name=Msa1437510
tig0028307 mRNA 54345 55374 54345 ID=Msa1437510-mRNA-1;Parent=Msa1437510;Name=Msa1437510-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|1|0.75|1|1|1|4|221|105
tig0028307 exon 54345 54670 54345 ID=Msa1437510-mRNA-1:exon:10714;Parent=Msa1437510-mRNA-1
tig0028307 exon 54986 55060 54986 ID=Msa1437510-mRNA-1:exon:10713;Parent=Msa1437510-mRNA-1
tig0028307 exon 55147 55239 55147 ID=Msa1437510-mRNA-1:exon:10712;Parent=Msa1437510-mRNA-1
tig0028307 exon 55330 55374 55330 ID=Msa1437510-mRNA-1:exon:10711;Parent=Msa1437510-mRNA-1
tig0028307 CDS 55330 55374 55330 ID=Msa1437510-mRNA-1:cds;Parent=Msa1437510-mRNA-1
tig0028307 CDS 55147 55239 55147 ID=Msa1437510-mRNA-1:cds;Parent=Msa1437510-mRNA-1
tig0028307 CDS 54986 55060 54986 ID=Msa1437510-mRNA-1:cds;Parent=Msa1437510-mRNA-1
tig0028307 CDS 54566 54670 54566 ID=Msa1437510-mRNA-1:cds;Parent=Msa1437510-mRNA-1
tig0028307 three_prime_UTR 54345 54565 54345 ID=Msa1437510-mRNA-1:three_prime_utr;Parent=Msa1437510-mRNA-1
Gene Sequence

>Msa1437510

ACAATTGAAGTTGGCACCCAAACTGCTTCTACCTTGAAGGGCCAAACTGAACAAATGGGCCGTATTGTTAATGAGCTTGATTCAATTCAGTTTTCAATAAAGAAGGCCTCCCAACTTGTTAAGGAGATTGGTAGACAGGTTGCTACAGACAAGTGCATCATGCTTTTTCTGTTTCTTATCGTCTGTGGTGTGATTGCCATTATTGTCGTGAAGATTGTGAATCCCAACAACAAAGATATTAGGGATATTCCAGGATTGGCGCCTCCAGTTCCCTCAAGGAGGCTTTTGTACGTACGGACTGGAGAACTTTTTGATTAA

Protein sequence

>Msa1437510

TIEVGTQTASTLKGQTEQMGRIVNELDSIQFSIKKASQLVKEIGRQVATDKCIMLFLFLIVCGVIAIIVVKIVNPNNKDIRDIPGLAPPVPSRRLLYVRTGELFD*