AlfalfaGEDB | Msa1444990

M. sativa cultivar zhongmu-4 / Msa1444990

KEGG: -
GO: GO:0000976 GO:0001067 GO:0001158 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0035326 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
PFAM: PF13837.9

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1444990	sp\|Q8H181\|GTL2_ARATH	40.842	475	168	16	103	483	103	558	6.69e-74	249

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1444990	G7L1T9	85.455	495	36	9	1	487	17	483	0.0	763

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TFs/TRs:

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Gene ID	Type	Classification
Msa1444990	TF	Trihelix

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
Msa0187640	Msa1444990	0.996106	1.811165e-223	-8.615850e-47
Msa1129260	Msa1444990	0.968026	4.271958e-128	-8.615850e-47
Msa1444990	Msa1459230	0.889630	2.039408e-73	-8.615850e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1444990	MtrunA17_Chr7g0273941	85.455	495	36	9	1	487	17	483	0.0	763
Msa1444990	MtrunA17_Chr1g0190851	45.850	506	203	18	32	492	34	513	3.22e-110	338
Msa1444990	MtrunA17_Chr2g0290511	31.250	256	137	9	245	481	114	349	1.41e-30	122
Msa1444990	MtrunA17_Chr6g0463941	55.238	105	38	2	385	489	464	559	1.04e-28	120
Msa1444990	MtrunA17_Chr6g0463941	41.837	98	47	2	385	482	55	142	5.78e-16	80.9
Msa1444990	MtrunA17_Chr1g0201941	43.750	96	44	2	385	480	57	142	9.72e-17	83.6
Msa1444990	MtrunA17_Chr1g0201941	35.227	88	48	2	233	317	274	355	8.87e-11	64.7
Msa1444990	MtrunA17_Chr1g0201911	42.424	99	47	2	385	483	55	143	1.15e-15	79.7
Msa1444990	MtrunA17_Chr7g0265831	39.000	100	50	3	385	483	115	204	6.21e-14	73.9
Msa1444990	MtrunA17_Chr7g0265831	24.542	273	160	8	86	317	96	363	9.81e-11	63.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
Msa1444990	AT5G28300.1	40.842	475	168	16	103	483	103	558	6.80e-75	249
Msa1444990	AT1G76890.2	37.363	273	135	9	220	484	243	487	5.80e-40	153
Msa1444990	AT1G76890.2	36.111	144	76	4	385	522	41	174	5.25e-16	81.3
Msa1444990	AT1G33240.2	34.444	270	132	8	244	481	227	483	1.14e-30	126
Msa1444990	AT1G33240.3	34.444	270	132	8	244	481	266	522	1.20e-30	126
Msa1444990	AT1G33240.3	42.708	96	45	2	385	480	62	147	4.68e-17	84.7
Msa1444990	AT1G76880.1	54.000	100	37	2	385	484	408	498	5.92e-27	115
Msa1444990	AT1G76880.1	41.071	112	52	3	376	483	48	149	9.39e-17	84.0
Msa1444990	AT1G33240.1	56.701	97	33	3	385	481	435	522	9.00e-26	112
Msa1444990	AT1G33240.1	42.708	96	45	2	385	480	62	147	3.99e-17	85.1

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Find 124 sgRNAs with CRISPR-Local

Find 309 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATTATTTATGGCTTATT+AGG	0.131965	tig0030876:-15304	Msa1444990:five_prime_UTR
AAGTTCGTCGTTAGTCCAGC+TGG	0.453547	tig0030876:+14799	None:intergenic
AATATGACCATTTGAATTGG+AGG	0.468145	tig0030876:+13519	None:intergenic
AATTAAGGGTATGTGGTGGA+AGG	0.587264	tig0030876:+15000	None:intergenic
AATTAGAGGATAGGAGAAGG+TGG	0.625991	tig0030876:-13885	Msa1444990:CDS
ACACTGTGATGATGACGACA+AGG	0.746516	tig0030876:-14370	Msa1444990:CDS
ACGGTGGTGGAGTTTGGGTC+TGG	0.489580	tig0030876:+14834	None:intergenic
ACTCTGTGTCACTTGAGCTA+GGG	0.543713	tig0030876:+13953	None:intergenic
AGAAAAGGTCAACGGATTCA+AGG	0.478087	tig0030876:-13636	Msa1444990:CDS
AGAAATTAGAGGATAGGAGA+AGG	0.501494	tig0030876:-13888	Msa1444990:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CTTCTAAACAAAGCTAAAAG+AGG	-	tig0030876:13197-13216	Msa1444990:three_prime_UTR	30.0%
GATCTTCAAGATCTTTATCA+AGG	-	tig0030876:13954-13973	Msa1444990:CDS	30.0%
GGAAAAAACAAGAGATTGAA+AGG	-	tig0030876:14279-14298	Msa1444990:CDS	30.0%
GTTCAAGAATTCAATGATGT+TGG	+	tig0030876:14354-14373	None:intergenic	30.0%
TCTGCTATCACATTAGAAAA+AGG	+	tig0030876:14467-14486	None:intergenic	30.0%
TAATGGAGAAATTAGAGGAT+AGG	-	tig0030876:14552-14571	Msa1444990:CDS	30.0%
AGAAGCAACAATAATGTTGA+AGG	-	tig0030876:14641-14660	Msa1444990:intron	30.0%
ATAGTAATAAAGGACCTTTG+TGG	-	tig0030876:14666-14685	Msa1444990:intron	30.0%
TAGTAATAAAGGACCTTTGT+GGG	-	tig0030876:14667-14686	Msa1444990:intron	30.0%
GAATAAGAAGAAAAGGTCAA+CGG	-	tig0030876:14802-14821	Msa1444990:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
tig0030876	gene	13086	15382	13086	ID=Msa1444990;Name=Msa1444990
tig0030876	mRNA	13086	15382	13086	ID=Msa1444990-mRNA-1;Parent=Msa1444990;Name=Msa1444990-mRNA-1;_AED=0.01;_eAED=0.01;_QI=260\|1\|1\|1\|1\|1\|2\|385\|523
tig0030876	exon	13086	14641	13086	ID=Msa1444990-mRNA-1:exon:14744;Parent=Msa1444990-mRNA-1
tig0030876	exon	14722	15382	14722	ID=Msa1444990-mRNA-1:exon:14743;Parent=Msa1444990-mRNA-1
tig0030876	five_prime_UTR	15123	15382	15123	ID=Msa1444990-mRNA-1:five_prime_utr;Parent=Msa1444990-mRNA-1
tig0030876	CDS	14722	15122	14722	ID=Msa1444990-mRNA-1:cds;Parent=Msa1444990-mRNA-1
tig0030876	CDS	13471	14641	13471	ID=Msa1444990-mRNA-1:cds;Parent=Msa1444990-mRNA-1
tig0030876	three_prime_UTR	13086	13470	13086	ID=Msa1444990-mRNA-1:three_prime_utr;Parent=Msa1444990-mRNA-1

Gene Sequence

>Msa1444990

ATGTTGGATGAAAGAGGAAAGCATTTTGAATTTGAGCAATTGAGGAGTACTACTAGTCTTCTTGGTTCGTCTTCTTCTTCTGTTGCAAACATCAACAGCACCTTCCACCACATACCCTTAATTCCCTCTACTTCTTCTTCCAATTCTCATTCTCCTCATCATCAATTTCCACTTCAAACTCAAACAAACCTCTTCCACCCATCAATACTATCATTGCCCCACCACTCTCCTAACACCACTACCTTTAATTCTAATTCAATCAACTTCCAGACCCAAACTCCACCACCGTTGATCATCGATTCCAGCTGGACTAACGACGAACTTCTTCTACTCTTCAAGATCACATCTACCATTCACAATTTCTTCCCAGATCAACTCATCACATGGGACCATGTCTCAAGTAAGCTAGCTGAGTTTGGGATCAAGAAGAGTGCTCAAAATTGTAAGGAGAAGTTTGAACATGAGAACACATCATTTATGAAGATTCATAACAATCATAATGATAATAATTTCCCCCGGTTTGTTAGTGATCTTCAAGATCTTTATCAAGGTGGTGGTGTTAGTGTTGATAATAAACAACCAAACCCTGACGAAAACAAAAATACCCAACACGAAGAAGATGATTCGAGAGATGATAGAGTAGAGACCAAACACTGTGATGATGACGACAAGGTGATGATGGAGAAATCAAAGAACAAAAAGAGGAAGAGGCGCCTTGATAGATTTGAAATGTTGAAGAGTTTTTGTGAGACAGTGGTTAACAAAATAATTGCACAACAAGAAGAAATACACAATAAGCTACTTGAAGACATGCTCAAAAGAGATCAAGAAAAGCTTGCTAGAGAGGAAGCATGGAAAAAACAAGAGATTGAAAGGATGAACATGATGGTACAACAACAACAAGCTATTGCAAGTGATAGACAAGCCAACATCATTGAATTCTTGAACAAATATCTAGCCACAGGATATTCTTCTTCTTCTTCACAATTGCACTCACAAAACCCTAATAATCCTTGTAACAATCTTAAATCAAAAACACCTTTTTCTAATGTGATAGCAGATCAAAACCCTAGCTCAAGTGACACAGAGTACTCAAATTCCACTTCTACCCTTGTTGTGCCTACAATAATGGAGAAATTAGAGGATAGGAGAAGGTGGCCAAGAGATGAAGTGTTGGCACTCATAAACCTCAAGTCTACTACAAGTGTTACAAACAGAAGCAACAATAATGTTGAAGGAAATAGTAATAAAGGACCTTTGTGGGAGAGAATCTCTGAAGGGATGTTTGAGTTGGGATACAAGAGAAGTGCAAAGAGGTGTAAAGAAAAATGGGAAAACATAAATAAGTATTTTAAAAAGACAAAGGATATTGTTGTGAATAAGAAGAAAAGGTCAACGGATTCAAGGACTTGTCCTTATTTTCATCAACTCAGTTCTTTGTATAATCAGCAGCAGCAGCAGCAGGGAAAAATAATTGATCCTCAATTAACCAAACATCCAACCTCCAATTCAAATGGTCATATTGTAGATGATCCACCTCAAGCTCAGTCTTCTGATTCTCCAATGGGGTAG

Protein sequence

>Msa1444990

MLDERGKHFEFEQLRSTTSLLGSSSSSVANINSTFHHIPLIPSTSSSNSHSPHHQFPLQTQTNLFHPSILSLPHHSPNTTTFNSNSINFQTQTPPPLIIDSSWTNDELLLLFKITSTIHNFFPDQLITWDHVSSKLAEFGIKKSAQNCKEKFEHENTSFMKIHNNHNDNNFPRFVSDLQDLYQGGGVSVDNKQPNPDENKNTQHEEDDSRDDRVETKHCDDDDKVMMEKSKNKKRKRRLDRFEMLKSFCETVVNKIIAQQEEIHNKLLEDMLKRDQEKLAREEAWKKQEIERMNMMVQQQQAIASDRQANIIEFLNKYLATGYSSSSSQLHSQNPNNPCNNLKSKTPFSNVIADQNPSSSDTEYSNSTSTLVVPTIMEKLEDRRRWPRDEVLALINLKSTTSVTNRSNNNVEGNSNKGPLWERISEGMFELGYKRSAKRCKEKWENINKYFKKTKDIVVNKKKRSTDSRTCPYFHQLSSLYNQQQQQQGKIIDPQLTKHPTSNSNGHIVDDPPQAQSSDSPMG*