AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1451220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1451220 MtrunA17_Chr5g0430371 96.579 380 11 1 22 399 22 401 0.0 754
Msa1451220 MtrunA17_Chr3g0105301 67.273 385 116 4 15 399 4 378 0.0 550
Msa1451220 MtrunA17_Chr2g0283581 50.964 363 149 7 38 397 22 358 3.28e-126 368
Msa1451220 MtrunA17_Chr4g0041011 45.269 391 178 7 7 397 9 363 2.05e-119 351
Msa1451220 MtrunA17_Chr3g0097891 49.422 346 153 5 53 398 28 351 2.34e-119 350
Msa1451220 MtrunA17_Chr4g0045951 46.939 392 169 7 8 398 10 363 3.69e-119 350
Msa1451220 MtrunA17_Chr4g0045941 49.580 357 152 6 42 398 23 351 6.66e-119 349
Msa1451220 MtrunA17_Chr2g0325481 47.230 343 156 4 56 398 50 367 2.39e-116 343
Msa1451220 MtrunA17_Chr3g0085081 46.875 352 162 5 46 397 22 348 2.51e-115 340
Msa1451220 MtrunA17_Chr3g0091031 45.954 346 163 4 52 397 27 348 4.96e-113 334
Msa1451220 MtrunA17_Chr3g0085071 45.821 347 164 4 51 397 41 363 1.43e-111 331
Msa1451220 MtrunA17_Chr2g0314801 45.676 370 175 8 29 394 3 350 1.24e-110 328
Msa1451220 MtrunA17_Chr5g0413851 43.577 397 175 10 10 397 3 359 5.40e-107 319
Msa1451220 MtrunA17_Chr1g0171171 39.601 351 189 5 50 397 45 375 3.01e-97 295
Msa1451220 MtrunA17_Chr1g0210771 39.481 347 186 5 54 397 57 382 8.17e-97 294
Msa1451220 MtrunA17_Chr7g0258851 40.390 359 180 7 56 398 248 588 1.74e-95 297
Msa1451220 MtrunA17_Chr2g0314791 43.099 355 159 9 53 398 26 346 1.43e-93 284
Msa1451220 MtrunA17_Chr1g0175671 37.435 382 205 7 33 398 147 510 2.94e-93 290
Msa1451220 MtrunA17_Chr1g0213281 38.889 360 193 6 53 399 172 517 2.63e-92 287
Msa1451220 MtrunA17_Chr5g0413271 37.709 358 203 4 46 398 135 477 8.45e-92 285
Msa1451220 MtrunA17_Chr4g0068111 39.674 368 192 7 45 398 103 454 8.25e-91 281
Msa1451220 MtrunA17_Chr4g0071391 36.798 356 197 6 54 397 107 446 1.35e-85 268
Msa1451220 MtrunA17_Chr4g0006991 37.853 354 196 9 56 397 86 427 5.66e-84 262
Msa1451220 MtrunA17_Chr7g0269991 37.960 353 186 8 56 397 137 467 7.15e-84 263
Msa1451220 MtrunA17_Chr8g0368461 35.550 391 203 8 35 398 161 529 3.86e-83 263
Msa1451220 MtrunA17_Chr3g0113671 38.268 358 197 7 53 397 103 449 1.04e-82 260
Msa1451220 MtrunA17_Chr7g0259711 36.119 371 183 9 55 398 90 433 4.25e-81 256
Msa1451220 MtrunA17_Chr4g0005491 36.011 361 205 8 54 399 60 409 6.19e-80 251
Msa1451220 MtrunA17_Chr3g0114101 38.056 360 191 9 56 399 89 432 3.27e-79 250
Msa1451220 MtrunA17_Chr5g0437291 36.769 359 200 8 56 398 165 512 4.57e-79 252
Msa1451220 MtrunA17_Chr8g0360521 35.440 364 208 8 47 398 62 410 4.59e-79 249
Msa1451220 MtrunA17_Chr2g0287901 37.088 364 187 10 55 398 96 437 1.45e-78 249
Msa1451220 MtrunA17_Chr5g0437601 34.368 419 236 11 3 399 25 426 2.34e-78 248
Msa1451220 MtrunA17_Chr7g0243301 34.444 360 210 7 54 398 62 410 4.76e-75 239
Msa1451220 MtrunA17_Chr7g0242701 37.326 359 199 10 52 397 82 427 7.33e-75 239
Msa1451220 MtrunA17_Chr4g0005481 36.000 350 201 6 55 394 107 443 2.67e-71 230
Msa1451220 MtrunA17_Chr3g0126021 33.508 382 205 10 35 398 110 460 7.23e-70 227
Msa1451220 MtrunA17_Chr1g0209921 43.396 265 141 8 18 278 7 266 4.36e-61 198
Msa1451220 MtrunA17_Chr8g0384151 31.522 368 202 7 50 398 81 417 1.48e-60 202
Msa1451220 MtrunA17_Chr1g0188191 30.239 377 227 11 38 393 216 577 2.13e-46 167
Msa1451220 MtrunA17_Chr8g0347321 31.880 367 221 12 50 397 83 439 4.35e-46 164
Msa1451220 MtrunA17_Chr5g0429211 82.222 90 16 0 105 194 189 278 8.48e-45 156
Msa1451220 MtrunA17_Chr5g0414051 28.715 397 251 10 11 393 38 416 3.73e-43 155
Msa1451220 MtrunA17_Chr5g0414061 29.558 362 226 10 48 393 86 434 4.02e-43 156
Msa1451220 MtrunA17_Chr5g0414091 30.387 362 225 10 48 393 77 427 9.49e-43 155
Msa1451220 MtrunA17_Chr8g0391481 30.084 359 219 13 53 393 24 368 1.51e-42 153
Msa1451220 MtrunA17_Chr6g0456691 29.722 360 221 10 54 397 76 419 2.28e-42 154
Msa1451220 MtrunA17_Chr6g0476041 28.191 376 231 11 43 394 151 511 2.36e-42 155
Msa1451220 MtrunA17_Chr7g0226991 27.809 356 231 9 53 393 54 398 9.10e-42 151
Msa1451220 MtrunA17_Chr8g0384421 28.030 396 211 14 38 397 115 472 1.64e-40 149
Msa1451220 MtrunA17_Chr1g0178081 35.749 207 113 5 50 236 44 250 4.08e-38 140
Msa1451220 MtrunA17_Chr1g0178081 48.649 74 30 2 328 398 274 342 5.37e-15 75.9
Msa1451220 MtrunA17_Chr6g0456681 27.692 390 212 13 46 397 51 408 4.76e-38 142
Msa1451220 MtrunA17_Chr2g0283851 30.504 377 202 16 54 397 52 401 1.77e-37 140
Msa1451220 MtrunA17_Chr8g0387921 27.838 370 207 10 56 393 74 415 2.32e-37 140
Msa1451220 MtrunA17_Chr5g0414041 28.283 396 255 11 16 393 45 429 7.70e-37 139
Msa1451220 MtrunA17_Chr3g0140371 27.473 364 217 11 54 393 16 356 1.11e-36 137
Msa1451220 MtrunA17_Chr4g0070341 28.571 371 207 17 55 397 131 471 3.04e-36 138
Msa1451220 MtrunA17_Chr6g0451321 28.238 386 234 13 33 397 94 457 5.93e-36 137
Msa1451220 MtrunA17_Chr4g0049421 27.095 358 224 11 56 393 48 388 8.07e-35 132
Msa1451220 MtrunA17_Chr5g0429201 93.220 59 4 0 281 339 22 80 2.42e-34 122
Msa1451220 MtrunA17_Chr6g0455901 27.968 379 213 16 47 393 62 412 3.92e-33 128
Msa1451220 MtrunA17_Chr7g0221291 53.333 90 42 0 56 145 79 168 4.45e-28 108
Msa1451220 MtrunA17_Chr7g0221271 41.732 127 74 0 56 182 79 205 1.63e-27 108
Msa1451220 MtrunA17_Chr3g0085101 45.000 120 53 3 241 360 1 107 1.35e-26 102
Msa1451220 MtrunA17_Chr5g0429191 95.556 45 2 0 346 390 1 45 9.87e-24 92.8
Msa1451220 MtrunA17_Chr2g0287931 38.710 124 63 3 117 227 3 126 1.05e-23 95.5
Msa1451220 MtrunA17_Chr1g0209931 37.624 101 47 2 298 398 9 93 1.80e-17 77.4
Msa1451220 MtrunA17_Chr2g0287911 51.724 58 21 2 54 111 59 109 6.18e-12 62.0
Msa1451220 MtrunA17_Chr7g0221281 26.887 212 126 10 193 393 39 232 1.39e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1451220 AT5G58600.1 54.657 408 167 4 2 399 3 402 2.12e-168 477
Msa1451220 AT2G30010.1 58.405 351 139 4 52 399 52 398 1.22e-155 445
Msa1451220 AT2G30010.2 58.405 351 139 4 52 399 95 441 1.61e-155 446
Msa1451220 AT1G29050.1 49.292 353 149 4 48 397 53 378 2.21e-123 362
Msa1451220 AT2G34070.1 48.732 355 157 5 44 397 53 383 2.97e-120 354
Msa1451220 AT5G58600.2 53.659 287 123 3 2 278 3 289 1.54e-116 341
Msa1451220 AT2G31110.2 47.953 342 154 4 56 397 45 362 4.30e-114 338
Msa1451220 AT2G42570.1 46.939 343 158 5 56 398 47 365 9.22e-114 337
Msa1451220 AT2G30900.1 47.977 346 157 7 56 399 43 367 4.19e-111 330
Msa1451220 AT3G14850.2 45.133 339 165 5 56 394 34 351 1.01e-105 316
Msa1451220 AT1G78710.1 43.429 350 169 8 52 398 34 357 1.00e-100 303
Msa1451220 AT3G54260.1 41.108 343 174 6 56 393 51 370 4.79e-95 290
Msa1451220 AT5G06700.1 39.385 358 187 7 55 398 254 595 1.86e-93 293
Msa1451220 AT3G12060.1 38.667 375 193 9 42 398 181 536 2.97e-92 288
Msa1451220 AT5G20590.1 40.056 357 183 9 56 398 141 480 2.30e-90 281
Msa1451220 AT1G60790.1 38.859 368 192 8 48 398 182 533 1.05e-89 281
Msa1451220 AT5G49340.1 38.421 380 185 11 44 398 87 442 2.37e-87 272
Msa1451220 AT5G01360.2 38.701 354 194 8 56 397 36 378 2.83e-85 265
Msa1451220 AT2G34070.2 47.253 273 119 5 126 397 1 249 3.39e-85 260
Msa1451220 AT5G01360.1 38.701 354 194 8 56 397 92 434 2.05e-84 264
Msa1451220 AT3G62390.1 35.813 363 203 8 48 399 130 473 4.07e-84 265
Msa1451220 AT3G55990.1 38.028 355 198 6 56 398 140 484 2.70e-82 260
Msa1451220 AT2G31110.3 50.459 218 107 1 56 273 45 261 8.16e-79 244
Msa1451220 AT5G19160.1 37.500 368 187 10 54 398 97 444 3.33e-78 249
Msa1451220 AT1G73140.1 35.467 375 208 10 41 398 49 406 3.83e-78 247
Msa1451220 AT2G40150.1 36.264 364 198 8 56 398 71 421 1.09e-77 246
Msa1451220 AT2G40320.1 34.135 416 239 9 3 399 25 424 1.65e-77 246
Msa1451220 AT2G30900.2 51.818 220 103 3 56 273 43 261 2.87e-76 238
Msa1451220 AT2G40160.1 35.000 360 207 9 56 399 77 425 1.01e-75 241
Msa1451220 AT5G01620.2 34.916 358 209 6 48 394 102 446 1.79e-75 241
Msa1451220 AT5G01620.1 34.916 358 209 6 48 394 102 446 1.79e-75 241
Msa1451220 AT5G01620.3 34.916 358 209 6 48 394 110 454 1.99e-75 241
Msa1451220 AT3G11030.1 36.288 361 197 8 56 399 106 450 3.47e-75 241
Msa1451220 AT2G40160.2 35.000 360 207 9 56 399 77 425 3.75e-75 240
Msa1451220 AT3G06080.2 37.088 364 191 11 54 398 101 445 3.87e-75 241
Msa1451220 AT3G11570.1 36.919 344 179 7 72 398 98 420 1.80e-72 233
Msa1451220 AT2G38320.1 32.787 366 220 7 48 398 51 405 2.70e-72 232
Msa1451220 AT3G14850.1 41.636 269 136 5 126 394 1 248 2.23e-70 222
Msa1451220 AT5G06230.2 36.337 344 183 8 71 398 42 365 6.41e-68 219
Msa1451220 AT5G06230.1 36.337 344 183 8 71 398 83 406 9.38e-68 220
Msa1451220 AT1G48880.1 33.240 358 196 9 54 398 113 440 6.31e-65 214
Msa1451220 AT2G31110.1 46.522 230 99 4 168 397 9 214 2.30e-64 205
Msa1451220 AT2G38320.2 32.727 330 197 6 83 398 4 322 3.06e-63 206
Msa1451220 AT5G20590.2 40.928 237 127 4 56 279 141 377 1.29e-60 201
Msa1451220 AT3G62390.2 35.802 243 145 3 48 279 130 372 3.32e-58 196
Msa1451220 AT5G19160.2 38.912 239 133 3 54 279 97 335 3.88e-58 193
Msa1451220 AT1G78710.2 33.711 353 146 13 52 398 27 297 2.29e-53 179
Msa1451220 AT3G06080.1 36.250 240 138 4 54 279 101 339 1.31e-50 174
Msa1451220 AT2G40160.3 35.043 234 141 4 56 278 77 310 1.63e-49 170
Msa1451220 AT5G06230.3 36.036 222 129 3 71 279 83 304 1.25e-46 162
Msa1451220 AT4G25360.1 31.044 364 213 12 53 393 172 520 1.26e-45 165
Msa1451220 AT4G25360.2 31.044 364 213 12 53 393 172 520 1.26e-45 165
Msa1451220 AT1G01430.1 29.024 379 224 9 46 398 89 448 1.38e-43 158
Msa1451220 AT5G51640.1 29.863 365 217 11 53 393 135 484 1.57e-43 159
Msa1451220 AT2G14530.1 28.193 415 240 14 12 397 21 406 5.70e-42 152
Msa1451220 AT5G64020.1 29.474 380 202 15 56 397 57 408 2.79e-40 148
Msa1451220 AT1G70230.1 28.255 361 220 10 52 397 75 411 2.89e-40 148
Msa1451220 AT5G20680.6 28.455 369 210 13 55 397 117 457 1.96e-38 144
Msa1451220 AT5G15900.1 29.189 370 217 12 51 397 62 409 3.76e-38 142
Msa1451220 AT5G20680.4 28.455 369 210 13 55 397 211 551 4.20e-38 144
Msa1451220 AT5G20680.1 28.455 369 210 13 55 397 211 551 4.20e-38 144
Msa1451220 AT5G20680.3 28.455 369 210 13 55 397 211 551 4.20e-38 144
Msa1451220 AT5G20680.5 28.455 369 210 13 55 397 211 551 4.20e-38 144
Msa1451220 AT5G15900.2 29.189 370 217 12 51 397 81 428 4.52e-38 142
Msa1451220 AT5G20680.2 28.261 368 212 13 55 397 193 533 5.06e-38 144
Msa1451220 AT5G15890.1 28.610 374 229 10 36 393 167 518 2.26e-37 142
Msa1451220 AT2G37720.1 28.158 380 197 14 55 397 142 482 2.62e-37 141
Msa1451220 AT4G01080.1 28.032 371 219 9 46 393 81 426 3.78e-37 140
Msa1451220 AT4G11090.1 28.451 355 225 10 56 393 79 421 1.92e-36 138
Msa1451220 AT5G64470.2 29.459 370 201 15 56 393 55 396 4.52e-36 136
Msa1451220 AT3G28150.1 28.177 362 219 9 56 397 70 410 1.46e-35 135
Msa1451220 AT5G64470.3 29.076 368 199 14 56 393 55 390 3.24e-35 134
Msa1451220 AT4G23790.1 29.752 363 216 12 54 393 77 423 9.13e-35 133
Msa1451220 AT3G02440.1 28.767 365 206 15 56 393 127 464 5.13e-34 132
Msa1451220 AT3G02440.2 28.767 365 206 15 56 393 135 472 6.06e-34 132
Msa1451220 AT5G64470.4 28.682 258 143 10 56 285 55 299 1.22e-20 92.0
Msa1451220 AT5G64470.1 28.682 258 143 10 56 285 55 299 1.39e-20 92.0

Find 91 sgRNAs with CRISPR-Local

Find 244 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTAGAAGATTGATAAAA+AGG 0.160341 tig0033270:+8864 None:intergenic
TCTCTCCACCTTTACATTCT+TGG 0.205076 tig0033270:-8323 Msa1451220:intron
ACTCAATTCACCACTGTAAA+TGG 0.243775 tig0033270:+5199 None:intergenic
TAGAACCCATGTGTTATTTC+AGG 0.260292 tig0033270:-7108 Msa1451220:CDS
ATTATTGTTTGCTTTCAAAC+TGG 0.270275 tig0033270:+8938 None:intergenic
AATGAAAAGAAGAAGCTATT+TGG 0.273436 tig0033270:+9006 None:intergenic
GTATGAAAACATGGGCTAAT+TGG 0.283621 tig0033270:-7154 Msa1451220:CDS
GGTTCTACTTCTGTCAATAT+TGG 0.289055 tig0033270:+7124 None:intergenic
GCTACCAAACGATCCATATC+AGG 0.293082 tig0033270:+7188 None:intergenic
TGTAGCCATTGGTGTCTTCC+TGG 0.300852 tig0033270:-5140 Msa1451220:CDS
TTCATTTGACAATTAAATTC+CGG 0.318356 tig0033270:+8828 None:intergenic
TTCCTGGTTTGCCTGATACT+TGG 0.320303 tig0033270:-5124 Msa1451220:CDS
GTGGTTTCCAACGATATTTA+AGG 0.330393 tig0033270:+8784 None:intergenic
ACAAATGAGAGTAGTTAATA+TGG 0.332485 tig0033270:-5300 Msa1451220:CDS
TCATTTGACAATTAAATTCC+GGG 0.335485 tig0033270:+8829 None:intergenic
AGGTGGGATTATGTAGAATT+AGG 0.337120 tig0033270:-7224 Msa1451220:CDS
ACATCAACATCTACTAAATA+AGG 0.339244 tig0033270:+7507 None:intergenic
TATGGATCGTTTGGTAGCTT+TGG 0.346986 tig0033270:-7183 Msa1451220:CDS
AAGTTCATTAGTACTTCATT+AGG 0.348991 tig0033270:-5066 Msa1451220:three_prime_UTR
AGTAGTTGATTCCAAGTATC+AGG 0.351359 tig0033270:+5113 None:intergenic
AGTAGTTGTGCATTGTTTGT+AGG 0.372824 tig0033270:-8912 Msa1451220:CDS
TGCATTGTTTGTAGGAAGTT+GGG 0.373791 tig0033270:-8904 Msa1451220:CDS
GTGCATTGTTTGTAGGAAGT+TGG 0.388888 tig0033270:-8905 Msa1451220:CDS
ATGTGGAGACACAATTGGTT+AGG 0.391848 tig0033270:-8354 Msa1451220:CDS
CTACAATCAGCTGAGTGATC+AGG 0.397666 tig0033270:+5158 None:intergenic
ACCAATTGTGTCTCCACATT+AGG 0.401722 tig0033270:+8358 None:intergenic
TATGAAAACATGGGCTAATT+GGG 0.412563 tig0033270:-7153 Msa1451220:CDS
TTACCCTGATATGGATCGTT+TGG 0.418196 tig0033270:-7192 Msa1451220:CDS
TTATTGTTTGCTTTCAAACT+GGG 0.418737 tig0033270:+8939 None:intergenic
AACTTGTGATGAGCTCAAAT+TGG 0.431782 tig0033270:-4847 Msa1451220:three_prime_UTR
TATTTAAGGTAATCAGAATC+AGG 0.448162 tig0033270:+8798 None:intergenic
GAATTTAATTGTCAAATGAA+TGG 0.454089 tig0033270:-8825 Msa1451220:CDS
CTGATTACCTTAAATATCGT+TGG 0.455897 tig0033270:-8791 Msa1451220:CDS
CGTTTGGTAGCTTTGGAAAG+AGG 0.463824 tig0033270:-7176 Msa1451220:CDS
TACAGTGTAAATTTCTCAAA+AGG 0.464861 tig0033270:-4919 Msa1451220:three_prime_UTR
TCTGTTGTTGTGCCACTGAT+TGG 0.469702 tig0033270:+5353 None:intergenic
TGTGGAGACACAATTGGTTA+GGG 0.482547 tig0033270:-8353 Msa1451220:CDS
TTATATGTAGCGAAAATGAA+TGG 0.497075 tig0033270:-5439 Msa1451220:intron
ATATGGTATATGCAGGACTA+TGG 0.498461 tig0033270:-7555 Msa1451220:intron
CACTCAATTGTGAGCTCCCA+AGG 0.498988 tig0033270:-8764 Msa1451220:intron
ACCTAATGTGGAGACACAAT+TGG 0.502101 tig0033270:-8359 Msa1451220:CDS
AGTGGCACAACAACAGACTT+TGG 0.502553 tig0033270:-5347 Msa1451220:CDS
TTATCAGAAATGAGGAAAGA+TGG 0.503627 tig0033270:-5230 Msa1451220:CDS
ATGTTTGTTGGTGATTCGTT+AGG 0.505244 tig0033270:-8427 Msa1451220:CDS
TGGGATTATGTAGAATTAGG+AGG 0.519702 tig0033270:-7221 Msa1451220:CDS
TAGACTCACCAAGAATGTAA+AGG 0.520499 tig0033270:+8315 None:intergenic
GTAGATGTTGATGTGGTCCA+AGG 0.533368 tig0033270:-7498 Msa1451220:CDS
TGGAAAGAGGTATGAAAACA+TGG 0.533428 tig0033270:-7163 Msa1451220:CDS
TACAATCAGCTGAGTGATCA+GGG 0.543582 tig0033270:+5159 None:intergenic
GGGATTATGTAGAATTAGGA+GGG 0.546280 tig0033270:-7220 Msa1451220:CDS
AATGGAATTCAGCTGTTGGT+AGG 0.546854 tig0033270:-5421 Msa1451220:CDS
GTGATGTCAAGAAGATATGC+AGG 0.548563 tig0033270:+5257 None:intergenic
TTATTTAGTAGATGTTGATG+TGG 0.549664 tig0033270:-7505 Msa1451220:CDS
TTCCAAGTATCAGGCAAACC+AGG 0.553910 tig0033270:+5122 None:intergenic
CTTGATGAGGCAGATAAGAA+TGG 0.554006 tig0033270:-7459 Msa1451220:CDS
TTGTTGGTGATTCGTTAGGA+AGG 0.556705 tig0033270:-8423 Msa1451220:CDS
GGACATTGGTGGAGTCATCA+AGG 0.558127 tig0033270:-7396 Msa1451220:CDS
ATTAATTAATCTAGCAACAA+AGG 0.558855 tig0033270:+4958 None:intergenic
TCACAATGTTATCAGAAATG+AGG 0.559566 tig0033270:-5238 Msa1451220:CDS
ATTCGTTAGGAAGGAATCAA+TGG 0.559645 tig0033270:-8414 Msa1451220:CDS
TGTTCAACACAGGACATTGG+TGG 0.565491 tig0033270:-7407 Msa1451220:CDS
ATGGAATTCAGCTGTTGGTA+GGG 0.570533 tig0033270:-5420 Msa1451220:CDS
AGTCATCAAGGATCACTTCA+AGG 0.572350 tig0033270:-7384 Msa1451220:CDS
AATTCACCACTGTAAATGGA+AGG 0.576652 tig0033270:+5203 None:intergenic
ATCTGCTGCAGCACCTAATG+TGG 0.578997 tig0033270:-8371 Msa1451220:CDS
ACTCAGCTGATTGTAGCCAT+TGG 0.580415 tig0033270:-5151 Msa1451220:CDS
AATGAATGGAATTCAGCTGT+TGG 0.581780 tig0033270:-5425 Msa1451220:CDS
GAGTCCTTACTCATAATGTG+TGG 0.582644 tig0033270:+7076 None:intergenic
CAGATAAGAATGGTGACACA+TGG 0.592161 tig0033270:-7449 Msa1451220:CDS
GGCACAACAACAGACTTTGG+AGG 0.599898 tig0033270:-5344 Msa1451220:CDS
CTACCAAACGATCCATATCA+GGG 0.604629 tig0033270:+7189 None:intergenic
CTTGGGAGCTCACAATTGAG+TGG 0.611295 tig0033270:+8765 None:intergenic
GAAAACATGGGCTAATTGGG+TGG 0.618678 tig0033270:-7150 Msa1451220:CDS
TAGATGTTGATGTGGTCCAA+GGG 0.619218 tig0033270:-7497 Msa1451220:CDS
ACAACAACAGACTTTGGAGG+AGG 0.621376 tig0033270:-5341 Msa1451220:CDS
AGTCCTTACTCATAATGTGT+GGG 0.625224 tig0033270:+7077 None:intergenic
TAGCTGTGATATCATAGACC+CGG 0.626468 tig0033270:-8847 Msa1451220:CDS
AAATTGCCTGAAATAACACA+TGG 0.627656 tig0033270:+7102 None:intergenic
GTCATCAAGGATCACTTCAA+GGG 0.627727 tig0033270:-7383 Msa1451220:intron
TGTTGTTCAACACAGGACAT+TGG 0.631668 tig0033270:-7410 Msa1451220:CDS
GGAAAGAGGTATGAAAACAT+GGG 0.632297 tig0033270:-7162 Msa1451220:CDS
AAGAATGTAAAGGTGGAGAG+AGG 0.636711 tig0033270:+8325 None:intergenic
ACTCACCAAGAATGTAAAGG+TGG 0.640751 tig0033270:+8318 None:intergenic
ACGACGACAAAGAATTGCTA+CGG 0.646290 tig0033270:-5386 Msa1451220:CDS
ATTCACCACTGTAAATGGAA+GGG 0.649803 tig0033270:+5204 None:intergenic
GATGTGTTGTTGTTCAACAC+AGG 0.663341 tig0033270:-7417 Msa1451220:CDS
GGTGAAACAGCACCAATCAG+TGG 0.681202 tig0033270:-5365 Msa1451220:CDS
GCAAACCAGGAAGACACCAA+TGG 0.684241 tig0033270:+5135 None:intergenic
GTGGAGACACAATTGGTTAG+GGG 0.689457 tig0033270:-8352 Msa1451220:CDS
GGACACCCTTCCATTTACAG+TGG 0.697469 tig0033270:-5209 Msa1451220:CDS
AATTGCCTGAAATAACACAT+GGG 0.716075 tig0033270:+7103 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATATATATATATATT+TGG + tig0033270:7808-7827 None:intergenic 0.0%
!!! TTTATTATTTTTTATACCTT+GGG + tig0033270:5183-5202 None:intergenic 10.0%
!!! TTTTATTATTTTTTATACCT+TGG + tig0033270:5184-5203 None:intergenic 10.0%
!!! AAAATTCTTGAAAATATTTT+GGG + tig0033270:6248-6267 None:intergenic 10.0%
!! AAAAATCAATTATTCATAAT+TGG + tig0033270:6898-6917 None:intergenic 10.0%
!! ATTATTAATAACTTGAAAAT+AGG - tig0033270:6938-6957 Msa1451220:intron 10.0%
!! TTATTAATAACTTGAAAATA+GGG - tig0033270:6939-6958 Msa1451220:intron 10.0%
!! TTAATTTAGTGATTTAAAAA+TGG - tig0033270:6971-6990 Msa1451220:intron 10.0%
!!! TAACAAAAAGTTTTAAATTG+TGG - tig0033270:5666-5685 Msa1451220:intron 15.0%
!! TCATATGCATATTATTTAAT+TGG + tig0033270:6220-6239 None:intergenic 15.0%
!! AATTAAATAATATGCATATG+AGG - tig0033270:6219-6238 Msa1451220:intron 15.0%
!! ATTAAATAATATGCATATGA+GGG - tig0033270:6220-6239 Msa1451220:intron 15.0%
!!! AAATTCTTGAAAATATTTTG+GGG + tig0033270:6247-6266 None:intergenic 15.0%
!!! CAAAATTCTTGAAAATATTT+TGG + tig0033270:6249-6268 None:intergenic 15.0%
!!! TTTTTTATAGATAGACAAAA+TGG - tig0033270:7535-7554 Msa1451220:CDS 15.0%
!! AATATGATGAATAAATTTCT+AGG - tig0033270:7752-7771 Msa1451220:intron 15.0%
!! TTATGATTGAAAAAGTTTAT+AGG - tig0033270:8203-8222 Msa1451220:intron 15.0%
!!! TAATTGCTGTTTTAATTATT+AGG + tig0033270:8347-8366 None:intergenic 15.0%
!! TTCATTTGACAATTAAATTC+CGG + tig0033270:5103-5122 None:intergenic 20.0%
!! GAATTTAATTGTCAAATGAA+TGG - tig0033270:5103-5122 Msa1451220:CDS 20.0%
!! TGAATCATTAGATAGATATA+AGG - tig0033270:6192-6211 Msa1451220:intron 20.0%
!! GAATCATTAGATAGATATAA+GGG - tig0033270:6193-6212 Msa1451220:intron 20.0%
!! TTAAATAATATGCATATGAG+GGG - tig0033270:6221-6240 Msa1451220:intron 20.0%
!!! TTTTCAAGAATTTTGATACA+TGG - tig0033270:6254-6273 Msa1451220:intron 20.0%
!!! CATTTTAGAGATGTTAAAAT+TGG - tig0033270:6345-6364 Msa1451220:intron 20.0%
!! TGTTAAAATTGGATATGATA+TGG - tig0033270:6356-6375 Msa1451220:intron 20.0%
!! TCTTGAATAAGCTTAAAAAA+GGG + tig0033270:6590-6609 None:intergenic 20.0%
!! TTCTTGAATAAGCTTAAAAA+AGG + tig0033270:6591-6610 None:intergenic 20.0%
!! ACTTAAGGAAAAAGTTAATT+TGG + tig0033270:7042-7061 None:intergenic 20.0%
!!! TGTAAATTTAGTTTGACTTA+AGG + tig0033270:7057-7076 None:intergenic 20.0%
!! AATTTGATTATTCTATTCTG+AGG - tig0033270:7173-7192 Msa1451220:CDS 20.0%
!! CATAAGAAAGATAAAGAATT+AGG - tig0033270:7313-7332 Msa1451220:intron 20.0%
!! AAAGAATTAGGAAGAAAAAA+TGG - tig0033270:7325-7344 Msa1451220:intron 20.0%
!! GATAGATATACTTTATATTG+AGG - tig0033270:7666-7685 Msa1451220:intron 20.0%
!! AACAAGTATAGTGAATAAAT+TGG - tig0033270:7718-7737 Msa1451220:intron 20.0%
!! ATAAGCTAAAAACAACTTAT+GGG + tig0033270:8160-8179 None:intergenic 20.0%
!! ATTAAACTGTTTATTCAAAC+AGG - tig0033270:8320-8339 Msa1451220:intron 20.0%
!! TTAAACTGTTTATTCAAACA+GGG - tig0033270:8321-8340 Msa1451220:intron 20.0%
!! GACTTAATTACATCAATATT+TGG - tig0033270:8401-8420 Msa1451220:CDS 20.0%
!! AACAAATTTATGCTAAGATT+TGG - tig0033270:8447-8466 Msa1451220:CDS 20.0%
!! ATTAATTAATCTAGCAACAA+AGG + tig0033270:8973-8992 None:intergenic 20.0%
! AATGAAAAGAAGAAGCTATT+TGG + tig0033270:4925-4944 None:intergenic 25.0%
! TTATTGTTTGCTTTCAAACT+GGG + tig0033270:4992-5011 None:intergenic 25.0%
! ATTATTGTTTGCTTTCAAAC+TGG + tig0033270:4993-5012 None:intergenic 25.0%
! AGCTAGAAGATTGATAAAAA+GGG + tig0033270:5066-5085 None:intergenic 25.0%
! TCATTTGACAATTAAATTCC+GGG + tig0033270:5102-5121 None:intergenic 25.0%
!! TATTTAAGGTAATCAGAATC+AGG + tig0033270:5133-5152 None:intergenic 25.0%
!!! TTTTAAGATAATGTAGTGTG+TGG - tig0033270:5378-5397 Msa1451220:CDS 25.0%
!!! TTGAATGATGATGTTTTTTG+TGG - tig0033270:5416-5435 Msa1451220:CDS 25.0%
!!! TTTTTTGTGGTGTTTTGTAA+TGG - tig0033270:5429-5448 Msa1451220:CDS 25.0%
! GATGTGATCATTGATATATT+CGG - tig0033270:5688-5707 Msa1451220:intron 25.0%
! AATCTTACCAACAAAAACTT+GGG + tig0033270:5990-6009 None:intergenic 25.0%
! AAATCTTACCAACAAAAACT+TGG + tig0033270:5991-6010 None:intergenic 25.0%
!!! TGTTGGTAAGATTTAGATTT+TGG - tig0033270:5997-6016 Msa1451220:intron 25.0%
!!! GATTTTGGTTTAAAAGAAAG+AGG - tig0033270:6012-6031 Msa1451220:intron 25.0%
! TGAATGAAACTTTACAAAGA+GGG + tig0033270:6088-6107 None:intergenic 25.0%
! ATGAATGAAACTTTACAAAG+AGG + tig0033270:6089-6108 None:intergenic 25.0%
! ACATCAACATCTACTAAATA+AGG + tig0033270:6424-6443 None:intergenic 25.0%
! TTATTTAGTAGATGTTGATG+TGG - tig0033270:6423-6442 Msa1451220:intron 25.0%
!!! AAGAATTTTAAGACTTGATG+AGG - tig0033270:6456-6475 Msa1451220:intron 25.0%
! ATCAAATTGCAACAAGATAT+GGG + tig0033270:7161-7180 None:intergenic 25.0%
! AATCAAATTGCAACAAGATA+TGG + tig0033270:7162-7181 None:intergenic 25.0%
!!! AAATTTTTTGGCTTGTTTGT+TGG - tig0033270:7199-7218 Msa1451220:CDS 25.0%
!! TCTTTCTTATGTGTTTTCAT+TGG + tig0033270:7304-7323 None:intergenic 25.0%
! TTTAACATTATTGCATTCTC+GGG + tig0033270:7439-7458 None:intergenic 25.0%
! GTTTAACATTATTGCATTCT+CGG + tig0033270:7440-7459 None:intergenic 25.0%
! AAGAGCTTTACTATCTAATT+TGG - tig0033270:7468-7487 Msa1451220:CDS 25.0%
!!! TATTTGGTAATGCTAGTAAA+AGG + tig0033270:7792-7811 None:intergenic 25.0%
! GATGTTAAGTTATGTAATAC+TGG - tig0033270:7919-7938 Msa1451220:intron 25.0%
!! TCTTTTCTTCATACTCAATT+GGG + tig0033270:7958-7977 None:intergenic 25.0%
!! TTCTTTTCTTCATACTCAAT+TGG + tig0033270:7959-7978 None:intergenic 25.0%
!!! AAAGAAGCTATTTTTTGCTA+AGG + tig0033270:8022-8041 None:intergenic 25.0%
!!! ACAAGCATTGATATGTAATA+AGG - tig0033270:8056-8075 Msa1451220:intron 25.0%
!!! CAAGCATTGATATGTAATAA+GGG - tig0033270:8057-8076 Msa1451220:intron 25.0%
!! ATAAACACACATGAGAATTT+TGG - tig0033270:8105-8124 Msa1451220:intron 25.0%
!! TAAACACACATGAGAATTTT+GGG - tig0033270:8106-8125 Msa1451220:intron 25.0%
! GATAAGCTAAAAACAACTTA+TGG + tig0033270:8161-8180 None:intergenic 25.0%
! CACATATATGATAAGTACTT+AGG - tig0033270:8284-8303 Msa1451220:intron 25.0%
!! TTTTCGCTACATATAAACAT+CGG + tig0033270:8485-8504 None:intergenic 25.0%
! TTATATGTAGCGAAAATGAA+TGG - tig0033270:8489-8508 Msa1451220:intron 25.0%
! ACAAATGAGAGTAGTTAATA+TGG - tig0033270:8628-8647 Msa1451220:intron 25.0%
! AAGTTCATTAGTACTTCATT+AGG - tig0033270:8862-8881 Msa1451220:CDS 25.0%
!! ACTTCATTAGGTATTTTACA+AGG - tig0033270:8874-8893 Msa1451220:CDS 25.0%
! TACAGTGTAAATTTCTCAAA+AGG - tig0033270:9009-9028 Msa1451220:CDS 25.0%
CAGCTAGAAGATTGATAAAA+AGG + tig0033270:5067-5086 None:intergenic 30.0%
CTGATTACCTTAAATATCGT+TGG - tig0033270:5137-5156 Msa1451220:CDS 30.0%
TTGAACATAATCACATGTGT+AGG + tig0033270:5282-5301 None:intergenic 30.0%
! TTTTGTAATGGTAGGTTCAA+TGG - tig0033270:5441-5460 Msa1451220:intron 30.0%
! GAGTTTTTGTTGAAGATGAA+AGG - tig0033270:5468-5487 Msa1451220:intron 30.0%
GAGTCTAAAAAAACATTCTG+AGG - tig0033270:5629-5648 Msa1451220:intron 30.0%
TGATATATTCGGAAGAATTG+CGG - tig0033270:5699-5718 Msa1451220:intron 30.0%
! GGATACAACTATTTTATTGC+TGG + tig0033270:6067-6086 None:intergenic 30.0%
TCATTCATGATATCACAAAG+AGG - tig0033270:6101-6120 Msa1451220:intron 30.0%
! GTCTTAAAATTCTTTTCCCT+TGG + tig0033270:6450-6469 None:intergenic 30.0%
! TTTGTTGATTAATTGAGTGC+AGG - tig0033270:6684-6703 Msa1451220:intron 30.0%
! TATGAAAACATGGGCTAATT+GGG - tig0033270:6775-6794 Msa1451220:intron 30.0%
AATTGCCTGAAATAACACAT+GGG + tig0033270:6828-6847 None:intergenic 30.0%
AAATTGCCTGAAATAACACA+TGG + tig0033270:6829-6848 None:intergenic 30.0%
! ACTCTTCTATGTCTCTATTT+TGG - tig0033270:6871-6890 Msa1451220:intron 30.0%
GTCAAGAATAGTCAAAAGAA+AGG + tig0033270:7131-7150 None:intergenic 30.0%
!!! ATTCTGAGGCTGAAATTTTT+TGG - tig0033270:7187-7206 Msa1451220:CDS 30.0%
TTCATAATTCTGTGACAAGA+TGG + tig0033270:7227-7246 None:intergenic 30.0%
AAAATATGTCAGCCTAGTTA+GGG - tig0033270:7276-7295 Msa1451220:intron 30.0%
ATAGTAAAGCTCTTTCTAAG+AGG + tig0033270:7462-7481 None:intergenic 30.0%
CAAAGACTAATCTCTTAAGT+CGG + tig0033270:7500-7519 None:intergenic 30.0%
ATTATAAAGTCACACACAAG+TGG - tig0033270:7565-7584 Msa1451220:intron 30.0%
AAGAATTGCTTCTTTACTTG+TGG - tig0033270:7974-7993 Msa1451220:intron 30.0%
TGATAAGTACTTAGGCTATA+AGG - tig0033270:8292-8311 Msa1451220:intron 30.0%
GATAAGTACTTAGGCTATAA+GGG - tig0033270:8293-8312 Msa1451220:intron 30.0%
TCACAATGTTATCAGAAATG+AGG - tig0033270:8690-8709 Msa1451220:intron 30.0%
TTATCAGAAATGAGGAAAGA+TGG - tig0033270:8698-8717 Msa1451220:intron 30.0%
AGTAGTTGTGCATTGTTTGT+AGG - tig0033270:5016-5035 Msa1451220:three_prime_UTR 35.0%
TGCATTGTTTGTAGGAAGTT+GGG - tig0033270:5024-5043 Msa1451220:three_prime_UTR 35.0%
GTGGTTTCCAACGATATTTA+AGG + tig0033270:5147-5166 None:intergenic 35.0%
! TTGTGGTGTTTTGTAATGGT+AGG - tig0033270:5433-5452 Msa1451220:intron 35.0%
! GGAAAAAGTGTGATGTTTGT+TGG - tig0033270:5489-5508 Msa1451220:intron 35.0%
!! ATGTTTGTTGGTGATTCGTT+AGG - tig0033270:5501-5520 Msa1451220:intron 35.0%
ATTCGTTAGGAAGGAATCAA+TGG - tig0033270:5514-5533 Msa1451220:intron 35.0%
TAGACTCACCAAGAATGTAA+AGG + tig0033270:5616-5635 None:intergenic 35.0%
! AACAGTCTGAAATGCCAAAT+GGG + tig0033270:5931-5950 None:intergenic 35.0%
! AAACAGTCTGAAATGCCAAA+TGG + tig0033270:5932-5951 None:intergenic 35.0%
TCATGATATCACAAAGAGGT+AGG - tig0033270:6105-6124 Msa1451220:intron 35.0%
CATGATATCACAAAGAGGTA+GGG - tig0033270:6106-6125 Msa1451220:intron 35.0%
!!! AGGTAGGGTCATATTTTTTG+TGG - tig0033270:6121-6140 Msa1451220:intron 35.0%
GCATAGTCAAAACATTCTAC+AGG + tig0033270:6313-6332 None:intergenic 35.0%
GGATATGATATGGTATATGC+AGG - tig0033270:6366-6385 Msa1451220:intron 35.0%
ATATGGTATATGCAGGACTA+TGG - tig0033270:6373-6392 Msa1451220:intron 35.0%
TTGATTAATTGAGTGCAGGT+GGG - tig0033270:6688-6707 Msa1451220:intron 35.0%
AGGTGGGATTATGTAGAATT+AGG - tig0033270:6704-6723 Msa1451220:intron 35.0%
TGGGATTATGTAGAATTAGG+AGG - tig0033270:6707-6726 Msa1451220:intron 35.0%
GGGATTATGTAGAATTAGGA+GGG - tig0033270:6708-6727 Msa1451220:intron 35.0%
! AGGGAATTTTTACCCTGATA+TGG - tig0033270:6727-6746 Msa1451220:intron 35.0%
! TGGAAAGAGGTATGAAAACA+TGG - tig0033270:6765-6784 Msa1451220:intron 35.0%
! GGAAAGAGGTATGAAAACAT+GGG - tig0033270:6766-6785 Msa1451220:intron 35.0%
! GTATGAAAACATGGGCTAAT+TGG - tig0033270:6774-6793 Msa1451220:intron 35.0%
GGTTCTACTTCTGTCAATAT+TGG + tig0033270:6807-6826 None:intergenic 35.0%
!! TAGAACCCATGTGTTATTTC+AGG - tig0033270:6820-6839 Msa1451220:intron 35.0%
AGTCCTTACTCATAATGTGT+GGG + tig0033270:6854-6873 None:intergenic 35.0%
GAAAATATGTCAGCCTAGTT+AGG - tig0033270:7275-7294 Msa1451220:intron 35.0%
! TTTTCATTGGATCCCTAACT+AGG + tig0033270:7291-7310 None:intergenic 35.0%
GCACATGAATAAGATAGTTC+TGG - tig0033270:7379-7398 Msa1451220:intron 35.0%
! GATATCCGACCTAACAATTT+TGG - tig0033270:7614-7633 Msa1451220:intron 35.0%
AGATGCCAAAATTGTTAGGT+CGG + tig0033270:7622-7641 None:intergenic 35.0%
!! TGATATGTAATAAGGGCGTT+TGG - tig0033270:8064-8083 Msa1451220:intron 35.0%
AATGAATGGAATTCAGCTGT+TGG - tig0033270:8503-8522 Msa1451220:intron 35.0%
ATTCACCACTGTAAATGGAA+GGG + tig0033270:8727-8746 None:intergenic 35.0%
AATTCACCACTGTAAATGGA+AGG + tig0033270:8728-8747 None:intergenic 35.0%
ACTCAATTCACCACTGTAAA+TGG + tig0033270:8732-8751 None:intergenic 35.0%
! AGTAGTTGATTCCAAGTATC+AGG + tig0033270:8818-8837 None:intergenic 35.0%
AACTTGTGATGAGCTCAAAT+TGG - tig0033270:9081-9100 Msa1451220:exon 35.0%
GTGCATTGTTTGTAGGAAGT+TGG - tig0033270:5023-5042 Msa1451220:three_prime_UTR 40.0%
TAGCTGTGATATCATAGACC+CGG - tig0033270:5081-5100 Msa1451220:exon 40.0%
!! TTGTTGGTGATTCGTTAGGA+AGG - tig0033270:5505-5524 Msa1451220:intron 40.0%
ACCTAATGTGGAGACACAAT+TGG - tig0033270:5569-5588 Msa1451220:intron 40.0%
ACCAATTGTGTCTCCACATT+AGG + tig0033270:5573-5592 None:intergenic 40.0%
ATGTGGAGACACAATTGGTT+AGG - tig0033270:5574-5593 Msa1451220:intron 40.0%
TGTGGAGACACAATTGGTTA+GGG - tig0033270:5575-5594 Msa1451220:intron 40.0%
AAGAATGTAAAGGTGGAGAG+AGG + tig0033270:5606-5625 None:intergenic 40.0%
TCTCTCCACCTTTACATTCT+TGG - tig0033270:5605-5624 Msa1451220:intron 40.0%
ACTCACCAAGAATGTAAAGG+TGG + tig0033270:5613-5632 None:intergenic 40.0%
! ATGAAGCACCTATGCAAACA+CGG + tig0033270:5858-5877 None:intergenic 40.0%
TGTTTGCATAGGTGCTTCAT+AGG - tig0033270:5858-5877 Msa1451220:intron 40.0%
!! CCAAATGGGGTATTGGTATT+TGG + tig0033270:5917-5936 None:intergenic 40.0%
CCAAATACCAATACCCCATT+TGG - tig0033270:5914-5933 Msa1451220:intron 40.0%
TGAAATGCCAAATGGGGTAT+TGG + tig0033270:5924-5943 None:intergenic 40.0%
! ACAGTCTGAAATGCCAAATG+GGG + tig0033270:5930-5949 None:intergenic 40.0%
! AAACTTGGGTAGTGGCATTA+TGG + tig0033270:5976-5995 None:intergenic 40.0%
ACCAACAAAAACTTGGGTAG+TGG + tig0033270:5984-6003 None:intergenic 40.0%
TAGATGTTGATGTGGTCCAA+GGG - tig0033270:6431-6450 Msa1451220:intron 40.0%
CTTGATGAGGCAGATAAGAA+TGG - tig0033270:6469-6488 Msa1451220:intron 40.0%
CAGATAAGAATGGTGACACA+TGG - tig0033270:6479-6498 Msa1451220:intron 40.0%
! GATGTGTTGTTGTTCAACAC+AGG - tig0033270:6511-6530 Msa1451220:intron 40.0%
TGTTGTTCAACACAGGACAT+TGG - tig0033270:6518-6537 Msa1451220:intron 40.0%
AGTCATCAAGGATCACTTCA+AGG - tig0033270:6544-6563 Msa1451220:intron 40.0%
GTCATCAAGGATCACTTCAA+GGG - tig0033270:6545-6564 Msa1451220:intron 40.0%
GTTGATTAATTGAGTGCAGG+TGG - tig0033270:6687-6706 Msa1451220:intron 40.0%
TTACCCTGATATGGATCGTT+TGG - tig0033270:6736-6755 Msa1451220:intron 40.0%
CTACCAAACGATCCATATCA+GGG + tig0033270:6742-6761 None:intergenic 40.0%
! TATGGATCGTTTGGTAGCTT+TGG - tig0033270:6745-6764 Msa1451220:intron 40.0%
TCTCCCACACATTATGAGTA+AGG - tig0033270:6848-6867 Msa1451220:intron 40.0%
GAGTCCTTACTCATAATGTG+TGG + tig0033270:6855-6874 None:intergenic 40.0%
GCTCATCAGATGAATGCAAA+TGG + tig0033270:7087-7106 None:intergenic 40.0%
GACTCTTTCAACAGAGTTCT+TGG - tig0033270:7401-7420 Msa1451220:CDS 40.0%
ATAAAGTCACACACAAGTGG+AGG - tig0033270:7568-7587 Msa1451220:intron 40.0%
TAAAGTCACACACAAGTGGA+GGG - tig0033270:7569-7588 Msa1451220:intron 40.0%
GCGAAGATGCCAAAATTGTT+AGG + tig0033270:7626-7645 None:intergenic 40.0%
! GCTAGTTTAGCTAGGATATG+TGG - tig0033270:7644-7663 Msa1451220:intron 40.0%
TGTCTTAATCTGATGAGCCA+AGG - tig0033270:7877-7896 Msa1451220:intron 40.0%
! CTTATCTGCATGAGCTTTTC+AGG - tig0033270:8175-8194 Msa1451220:intron 40.0%
AATGGAATTCAGCTGTTGGT+AGG - tig0033270:8507-8526 Msa1451220:intron 40.0%
ATGGAATTCAGCTGTTGGTA+GGG - tig0033270:8508-8527 Msa1451220:intron 40.0%
ACGACGACAAAGAATTGCTA+CGG - tig0033270:8542-8561 Msa1451220:intron 40.0%
GTGATGTCAAGAAGATATGC+AGG + tig0033270:8674-8693 None:intergenic 40.0%
TACAATCAGCTGAGTGATCA+GGG + tig0033270:8772-8791 None:intergenic 40.0%
TTAAGTGTCAGTGTCGTGTC+TGG - tig0033270:5314-5333 Msa1451220:CDS 45.0%
GTGGAGACACAATTGGTTAG+GGG - tig0033270:5576-5595 Msa1451220:intron 45.0%
TCGTATGACCGTGTTTGCAT+AGG - tig0033270:5847-5866 Msa1451220:intron 45.0%
! GCCACTACCCAAGTTTTTGT+TGG - tig0033270:5980-5999 Msa1451220:intron 45.0%
GTAGATGTTGATGTGGTCCA+AGG - tig0033270:6430-6449 Msa1451220:intron 45.0%
TGTTCAACACAGGACATTGG+TGG - tig0033270:6521-6540 Msa1451220:intron 45.0%
TCAAGGATCACTTCAAGGGT+AGG - tig0033270:6549-6568 Msa1451220:intron 45.0%
GCTACCAAACGATCCATATC+AGG + tig0033270:6743-6762 None:intergenic 45.0%
!! CGTTTGGTAGCTTTGGAAAG+AGG - tig0033270:6752-6771 Msa1451220:intron 45.0%
! GAAAACATGGGCTAATTGGG+TGG - tig0033270:6778-6797 Msa1451220:intron 45.0%
TCTGATGAGCTGCTTAGATC+TGG - tig0033270:7097-7116 Msa1451220:CDS 45.0%
AGCTGCTTAGATCTGGAAGT+GGG - tig0033270:7104-7123 Msa1451220:CDS 45.0%
AAGCTCTGCACATTGAAGTG+CGG + tig0033270:7251-7270 None:intergenic 45.0%
AAAGTCACACACAAGTGGAG+GGG - tig0033270:7570-7589 Msa1451220:intron 45.0%
TAGGTCGGATATCATGACCA+GGG + tig0033270:7607-7626 None:intergenic 45.0%
TTAGGTCGGATATCATGACC+AGG + tig0033270:7608-7627 None:intergenic 45.0%
! GCATCTTCGCTAGTTTAGCT+AGG - tig0033270:7636-7655 Msa1451220:intron 45.0%
CTTAATCTGATGAGCCAAGG+TGG - tig0033270:7880-7899 Msa1451220:intron 45.0%
CTTGTGGCATTAACTCACTG+AGG - tig0033270:7990-8009 Msa1451220:intron 45.0%
! TCTGTTGTTGTGCCACTGAT+TGG + tig0033270:8578-8597 None:intergenic 45.0%
AGTGGCACAACAACAGACTT+TGG - tig0033270:8581-8600 Msa1451220:intron 45.0%
! ACAACAACAGACTTTGGAGG+AGG - tig0033270:8587-8606 Msa1451220:intron 45.0%
CTACAATCAGCTGAGTGATC+AGG + tig0033270:8773-8792 None:intergenic 45.0%
ACTCAGCTGATTGTAGCCAT+TGG - tig0033270:8777-8796 Msa1451220:CDS 45.0%
! TTCCTGGTTTGCCTGATACT+TGG - tig0033270:8804-8823 Msa1451220:CDS 45.0%
TTCCAAGTATCAGGCAAACC+AGG + tig0033270:8809-8828 None:intergenic 45.0%
!!! AAAAAATTTGATTTTTTTTT+TGG - tig0033270:6655-6674 Msa1451220:intron 5.0%
!!! AAAAATTTGATTTTTTTTTT+GGG - tig0033270:6656-6675 Msa1451220:intron 5.0%
CTTGGGAGCTCACAATTGAG+TGG + tig0033270:5166-5185 None:intergenic 50.0%
CACTCAATTGTGAGCTCCCA+AGG - tig0033270:5164-5183 Msa1451220:CDS 50.0%
GATGTGCCTAGTGTCTGACA+CGG - tig0033270:5230-5249 Msa1451220:CDS 50.0%
ATGTGCCTAGTGTCTGACAC+GGG - tig0033270:5231-5250 Msa1451220:CDS 50.0%
TCGTGTCTGGTGTTCGTGTT+TGG - tig0033270:5327-5346 Msa1451220:CDS 50.0%
CGTGTCTGGTGTTCGTGTTT+GGG - tig0033270:5328-5347 Msa1451220:CDS 50.0%
ATCTGCTGCAGCACCTAATG+TGG - tig0033270:5557-5576 Msa1451220:intron 50.0%
TGCAGCCTTGATTGCAGTTG+CGG - tig0033270:5736-5755 Msa1451220:intron 50.0%
AGACACCGCAACTGCAATCA+AGG + tig0033270:5744-5763 None:intergenic 50.0%
GGACATTGGTGGAGTCATCA+AGG - tig0033270:6532-6551 Msa1451220:intron 50.0%
GAGCTGCTTAGATCTGGAAG+TGG - tig0033270:7103-7122 Msa1451220:CDS 50.0%
CATGTGTGATGTTGCCACCT+TGG + tig0033270:7897-7916 None:intergenic 50.0%
GGTGAAACAGCACCAATCAG+TGG - tig0033270:8563-8582 Msa1451220:intron 50.0%
GGCACAACAACAGACTTTGG+AGG - tig0033270:8584-8603 Msa1451220:intron 50.0%
GGACACCCTTCCATTTACAG+TGG - tig0033270:8719-8738 Msa1451220:intron 50.0%
! TGTAGCCATTGGTGTCTTCC+TGG - tig0033270:8788-8807 Msa1451220:CDS 50.0%
GCAAACCAGGAAGACACCAA+TGG + tig0033270:8796-8815 None:intergenic 50.0%
AGACACCCGTGTCAGACACT+AGG + tig0033270:5239-5258 None:intergenic 55.0%
TCACACACAAGTGGAGGGGT+CGG - tig0033270:7574-7593 Msa1451220:intron 55.0%
ACACGGGTGTCTGACACGTG+TGG - tig0033270:5247-5266 Msa1451220:CDS 60.0%
CACACACAAGTGGAGGGGTC+GGG - tig0033270:7575-7594 Msa1451220:intron 60.0%
GAGGGGTCGGGTTCAAACCC+TGG - tig0033270:7587-7606 Msa1451220:intron 65.0%
Chromosome Type Strat End Strand Name
tig0033270 gene 4846 9104 4846 ID=Msa1451220;Name=Msa1451220
tig0033270 mRNA 4846 9104 4846 ID=Msa1451220-mRNA-1;Parent=Msa1451220;Name=Msa1451220-mRNA-1;_AED=0.07;_eAED=0.07;_QI=11|1|1|1|1|1|5|245|399
tig0033270 exon 4846 5451 4846 ID=Msa1451220-mRNA-1:exon:17915;Parent=Msa1451220-mRNA-1
tig0033270 exon 7086 7244 7086 ID=Msa1451220-mRNA-1:exon:17914;Parent=Msa1451220-mRNA-1
tig0033270 exon 7384 7562 7384 ID=Msa1451220-mRNA-1:exon:17913;Parent=Msa1451220-mRNA-1
tig0033270 exon 8324 8495 8324 ID=Msa1451220-mRNA-1:exon:17912;Parent=Msa1451220-mRNA-1
tig0033270 exon 8765 9104 8765 ID=Msa1451220-mRNA-1:exon:17911;Parent=Msa1451220-mRNA-1
tig0033270 five_prime_UTR 9094 9104 9094 ID=Msa1451220-mRNA-1:five_prime_utr;Parent=Msa1451220-mRNA-1
tig0033270 CDS 8765 9093 8765 ID=Msa1451220-mRNA-1:cds;Parent=Msa1451220-mRNA-1
tig0033270 CDS 8324 8495 8324 ID=Msa1451220-mRNA-1:cds;Parent=Msa1451220-mRNA-1
tig0033270 CDS 7384 7562 7384 ID=Msa1451220-mRNA-1:cds;Parent=Msa1451220-mRNA-1
tig0033270 CDS 7086 7244 7086 ID=Msa1451220-mRNA-1:cds;Parent=Msa1451220-mRNA-1
tig0033270 CDS 5091 5451 5091 ID=Msa1451220-mRNA-1:cds;Parent=Msa1451220-mRNA-1
tig0033270 three_prime_UTR 4846 5090 4846 ID=Msa1451220-mRNA-1:three_prime_utr;Parent=Msa1451220-mRNA-1
Gene Sequence

>Msa1451220

ATGTCTCTTTCTTGTTATGTGTTTGTTTCATTCTTCTCTCTTCTTTTTCTTCTTTCTCTTCAATGCCAAATAGCTTCTTCTTTTCATTATCATTATCATCATCATCATCATGAACAAGTTTATCATCAAAAACCCAGTTTGAAAGCAAACAATAATCAAAGTAGTTGTGCATTGTTTGTAGGAAGTTGGGTTTATGATGAAAGTTACCCTTTTTATCAATCTTCTAGCTGTGATATCATAGACCCGGAATTTAATTGTCAAATGAATGGTCGTCCTGATTCTGATTACCTTAAATATCGTTGGAAACCACTCAATTGTGAGCTCCCAAGGTTCAATGGAGTTGAGTTTTTGTTGAAGATGAAAGGAAAAAGTGTGATGTTTGTTGGTGATTCGTTAGGAAGGAATCAATGGCAGTCTTTGATTTGTATGATATCTGCTGCAGCACCTAATGTGGAGACACAATTGGTTAGGGGAGATCCTCTCTCCACCTTTACATTCTTGGACTATGGTGTGAAAATTTCATTTCATAGAGCTCCTTATTTAGTAGATGTTGATGTGGTCCAAGGGAAAAGAATTTTAAGACTTGATGAGGCAGATAAGAATGGTGACACATGGAAGAATGTTGATGTGTTGTTGTTCAACACAGGACATTGGTGGAGTCATCAAGGATCACTTCAAGGGTGGGATTATGTAGAATTAGGAGGGAATTTTTACCCTGATATGGATCGTTTGGTAGCTTTGGAAAGAGGTATGAAAACATGGGCTAATTGGGTGGATGCCAATATTGACAGAAGTAGAACCCATGTGTTATTTCAGGCAATTTCTCCCACACATTATGACGAAAATGAATGGAATTCAGCTGTTGGTAGGGCAACAAGTGTAACGACGACAAAGAATTGCTACGGTGAAACAGCACCAATCAGTGGCACAACAACAGACTTTGGAGGAGGAGAAACATACACTGATCAACAAATGAGAGTAGTTAATATGGTGATTCGAGAAATGCGCGATCCTGCATATCTTCTTGACATCACAATGTTATCAGAAATGAGGAAAGATGGACACCCTTCCATTTACAGTGGTGAATTGAGTTCTCAGCAAAGAGCTGACCCTGATCACTCAGCTGATTGTAGCCATTGGTGTCTTCCTGGTTTGCCTGATACTTGGAATCAACTACTCTATGTTGCTTTATTCTATTGA
Protein sequence

>Msa1451220

MSLSCYVFVSFFSLLFLLSLQCQIASSFHYHYHHHHHEQVYHQKPSLKANNNQSSCALFVGSWVYDESYPFYQSSSCDIIDPEFNCQMNGRPDSDYLKYRWKPLNCELPRFNGVEFLLKMKGKSVMFVGDSLGRNQWQSLICMISAAAPNVETQLVRGDPLSTFTFLDYGVKISFHRAPYLVDVDVVQGKRILRLDEADKNGDTWKNVDVLLFNTGHWWSHQGSLQGWDYVELGGNFYPDMDRLVALERGMKTWANWVDANIDRSRTHVLFQAISPTHYDENEWNSAVGRATSVTTTKNCYGETAPISGTTTDFGGGETYTDQQMRVVNMVIREMRDPAYLLDITMLSEMRKDGHPSIYSGELSSQQRADPDHSADCSHWCLPGLPDTWNQLLYVALFY*