Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1452530 | sp|A2WSH0|AB36G_ORYSI | 68.306 | 183 | 56 | 1 | 1 | 183 | 1277 | 1457 | 5.57e-82 | 267 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0225830 | Msa1452530 | 0.963905 | 1.162615e-122 | -8.615850e-47 |
| Msa0260540 | Msa1452530 | 0.963636 | 2.503454e-122 | -8.615850e-47 |
| Msa0267790 | Msa1452530 | 0.800020 | 1.752701e-48 | -8.615850e-47 |
| Msa0271540 | Msa1452530 | 0.820231 | 7.650349e-53 | -8.615850e-47 |
| Msa0305840 | Msa1452530 | 0.815195 | 1.046993e-51 | -8.615850e-47 |
| Msa0319260 | Msa1452530 | 0.809594 | 1.751677e-50 | -8.615850e-47 |
| Msa0443020 | Msa1452530 | 0.801666 | 8.083387e-49 | -8.615850e-47 |
| Msa0506860 | Msa1452530 | 0.813426 | 2.575212e-51 | -8.615850e-47 |
| Msa0574600 | Msa1452530 | 0.802680 | 5.002042e-49 | -8.615850e-47 |
| Msa0614380 | Msa1452530 | 0.806842 | 6.758634e-50 | -8.615850e-47 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1452530 | MtrunA17_Chr2g0332241 | 100.000 | 183 | 0 | 0 | 1 | 183 | 1246 | 1428 | 5.45e-125 | 383 |
| Msa1452530 | MtrunA17_Chr2g0332221 | 98.907 | 183 | 2 | 0 | 1 | 183 | 1245 | 1427 | 2.29e-123 | 379 |
| Msa1452530 | MtrunA17_Chr2g0332211 | 98.361 | 183 | 3 | 0 | 1 | 183 | 1329 | 1511 | 8.00e-123 | 378 |
| Msa1452530 | MtrunA17_Chr4g0004001 | 74.138 | 174 | 44 | 1 | 10 | 183 | 16 | 188 | 1.11e-96 | 278 |
| Msa1452530 | MtrunA17_Chr4g0003981 | 74.317 | 183 | 46 | 1 | 1 | 183 | 1256 | 1437 | 1.81e-94 | 299 |
| Msa1452530 | MtrunA17_Chr7g0262231 | 63.587 | 184 | 66 | 1 | 1 | 183 | 1273 | 1456 | 7.27e-82 | 264 |
| Msa1452530 | MtrunA17_Chr7g0262221 | 60.513 | 195 | 65 | 2 | 1 | 183 | 1244 | 1438 | 1.28e-77 | 251 |
| Msa1452530 | MtrunA17_Chr7g0262241 | 60.326 | 184 | 72 | 1 | 1 | 183 | 1270 | 1453 | 1.49e-77 | 251 |
| Msa1452530 | MtrunA17_Chr1g0148841 | 60.109 | 183 | 71 | 1 | 1 | 183 | 1267 | 1447 | 1.69e-76 | 248 |
| Msa1452530 | MtrunA17_Chr4g0003971 | 66.120 | 183 | 61 | 1 | 1 | 183 | 1215 | 1396 | 2.93e-75 | 245 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa1452530 | AT1G15520.1 | 64.481 | 183 | 64 | 1 | 1 | 183 | 1242 | 1423 | 2.38e-79 | 257 |
| Msa1452530 | AT2G26910.1 | 58.602 | 186 | 74 | 1 | 1 | 183 | 1235 | 1420 | 4.24e-72 | 236 |
| Msa1452530 | AT1G59870.1 | 58.065 | 186 | 75 | 1 | 1 | 183 | 1284 | 1469 | 1.33e-64 | 215 |
| Msa1452530 | AT1G15210.1 | 56.989 | 186 | 77 | 1 | 1 | 183 | 1257 | 1442 | 6.90e-64 | 213 |
| Msa1452530 | AT3G16340.1 | 50.538 | 186 | 89 | 1 | 1 | 183 | 1231 | 1416 | 1.74e-60 | 203 |
| Msa1452530 | AT3G16340.2 | 50.538 | 186 | 89 | 1 | 1 | 183 | 1226 | 1411 | 2.06e-60 | 202 |
| Msa1452530 | AT1G66950.1 | 51.892 | 185 | 87 | 1 | 1 | 183 | 1270 | 1454 | 6.21e-59 | 198 |
| Msa1452530 | AT2G36380.1 | 50.811 | 185 | 89 | 1 | 1 | 183 | 1269 | 1453 | 6.58e-56 | 190 |
| Msa1452530 | AT2G29940.1 | 47.849 | 186 | 92 | 2 | 1 | 183 | 1243 | 1426 | 8.21e-56 | 189 |
| Msa1452530 | AT3G30842.1 | 44.262 | 183 | 100 | 1 | 1 | 183 | 1238 | 1418 | 4.77e-53 | 182 |
Find 400 sgRNAs with CRISPR-Local
Find 1418 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACAGGTGGATATATTGA+TGG | 0.451503 | tig0033997:-9030 | Msa1452530:five_prime_UTR |
| AAAAGGATCAAGCACAGTAC+TGG | 0.395784 | tig0033997:-11007 | Msa1452530:five_prime_UTR |
| AAAAGTAATGTCATCCTACG+AGG | 0.708513 | tig0033997:+17239 | Msa1452530:intergenic |
| AAAAGTCGAGAACATATTCG+CGG | 0.617411 | tig0033997:+11099 | Msa1452530:intergenic |
| AAAATACTGTCATAAGACTT+TGG | 0.453173 | tig0033997:+23042 | Msa1452530:intergenic |
| AAAATTATCTCGATACCCGA+GGG | 0.649603 | tig0033997:-9718 | Msa1452530:five_prime_UTR |
| AAACCTTAGCTTTCTCAGCA+AGG | 0.571427 | tig0033997:-12072 | Msa1452530:five_prime_UTR |
| AAACTGTAACATGAGGTGAA+TGG | 0.568363 | tig0033997:+8929 | Msa1452530:intergenic |
| AAACTTCGTCCTACAAGTTA+AGG | 0.432395 | tig0033997:+18409 | Msa1452530:intergenic |
| AAAGCTCCGGTGACCGGAAA+TGG | 0.372638 | tig0033997:+24117 | Msa1452530:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CGTGATTTCCTTGATTAATT+AGG | - | tig0033997:3687-3706 | Msa1452530:three_prime_UTR | 30.0% | |
| CTATCTATTTAAGAGATCGT+AGG | - | tig0033997:3874-3893 | Msa1452530:intron | 30.0% | |
| ATCTATTTAAGAGATCGTAG+GGG | - | tig0033997:3876-3895 | Msa1452530:intron | 30.0% | |
| TTGTCCACTAAAAAACTAGA+TGG | - | tig0033997:3905-3924 | Msa1452530:intron | 30.0% | |
| AAAGTTAAAATGGCTTATGC+TGG | - | tig0033997:3987-4006 | Msa1452530:intron | 30.0% | |
| ATTTGTAAAGCTTCCAACAA+TGG | - | tig0033997:4303-4322 | Msa1452530:intron | 30.0% | |
| TACTTCGGTTTACTTAAAGT+TGG | - | tig0033997:4642-4661 | Msa1452530:intron | 30.0% | |
| AATCAAGAAAATCACAGTTG+TGG | + | tig0033997:5331-5350 | Msa1452530:intergenic | 30.0% | |
| ACCATACAACAAAGTCAATT+TGG | + | tig0033997:5677-5696 | Msa1452530:intergenic | 30.0% | |
| TGCCAAAAATCAAAAGATAG+AGG | + | tig0033997:5770-5789 | Msa1452530:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| tig0033997 | gene | 3523 | 24255 | 3523 | ID=Msa1452530;Name=Msa1452530 |
| tig0033997 | mRNA | 3523 | 24255 | 3523 | ID=Msa1452530-mRNA-1;Parent=Msa1452530;Name=Msa1452530-mRNA-1;_AED=0.13;_eAED=0.13;_QI=5039|1|1|1|0.75|0.66|21|230|183 |
| tig0033997 | exon | 3523 | 3731 | 3523 | ID=Msa1452530-mRNA-1:exon:18666;Parent=Msa1452530-mRNA-1 |
| tig0033997 | exon | 6699 | 6983 | 6699 | ID=Msa1452530-mRNA-1:exon:18665;Parent=Msa1452530-mRNA-1 |
| tig0033997 | exon | 7075 | 7329 | 7075 | ID=Msa1452530-mRNA-1:exon:18664;Parent=Msa1452530-mRNA-1 |
| tig0033997 | exon | 7456 | 7588 | 7456 | ID=Msa1452530-mRNA-1:exon:18663;Parent=Msa1452530-mRNA-1 |
| tig0033997 | exon | 7905 | 8382 | 7905 | ID=Msa1452530-mRNA-1:exon:18662;Parent=Msa1452530-mRNA-1 |
| tig0033997 | exon | 8492 | 8782 | 8492 | ID=Msa1452530-mRNA-1:exon:18661;Parent=Msa1452530-mRNA-1 |
| tig0033997 | exon | 8864 | 9196 | 8864 | ID=Msa1452530-mRNA-1:exon:18660;Parent=Msa1452530-mRNA-1 |
| tig0033997 | exon | 9304 | 9428 | 9304 | ID=Msa1452530-mRNA-1:exon:18659;Parent=Msa1452530-mRNA-1 |
| tig0033997 | exon | 9528 | 9759 | 9528 | ID=Msa1452530-mRNA-1:exon:18658;Parent=Msa1452530-mRNA-1 |
| tig0033997 | exon | 9852 | 9955 | 9852 | ID=Msa1452530-mRNA-1:exon:18657;Parent=Msa1452530-mRNA-1 |
| tig0033997 | exon | 10086 | 10246 | 10086 | ID=Msa1452530-mRNA-1:exon:18656;Parent=Msa1452530-mRNA-1 |
| tig0033997 | exon | 10359 | 10675 | 10359 | ID=Msa1452530-mRNA-1:exon:18655;Parent=Msa1452530-mRNA-1 |
| tig0033997 | exon | 10758 | 11432 | 10758 | ID=Msa1452530-mRNA-1:exon:18654;Parent=Msa1452530-mRNA-1 |
| tig0033997 | exon | 11743 | 11796 | 11743 | ID=Msa1452530-mRNA-1:exon:18653;Parent=Msa1452530-mRNA-1 |
| tig0033997 | exon | 11880 | 11956 | 11880 | ID=Msa1452530-mRNA-1:exon:18652;Parent=Msa1452530-mRNA-1 |
| tig0033997 | exon | 12047 | 12206 | 12047 | ID=Msa1452530-mRNA-1:exon:18651;Parent=Msa1452530-mRNA-1 |
| tig0033997 | exon | 17169 | 17342 | 17169 | ID=Msa1452530-mRNA-1:exon:18650;Parent=Msa1452530-mRNA-1 |
| tig0033997 | exon | 17468 | 17588 | 17468 | ID=Msa1452530-mRNA-1:exon:18649;Parent=Msa1452530-mRNA-1 |
| tig0033997 | exon | 17690 | 18813 | 17690 | ID=Msa1452530-mRNA-1:exon:18648;Parent=Msa1452530-mRNA-1 |
| tig0033997 | exon | 22849 | 23175 | 22849 | ID=Msa1452530-mRNA-1:exon:18647;Parent=Msa1452530-mRNA-1 |
| tig0033997 | exon | 24070 | 24255 | 24070 | ID=Msa1452530-mRNA-1:exon:18646;Parent=Msa1452530-mRNA-1 |
| tig0033997 | five_prime_UTR | 24070 | 24255 | 24070 | ID=Msa1452530-mRNA-1:five_prime_utr;Parent=Msa1452530-mRNA-1 |
| tig0033997 | five_prime_UTR | 22849 | 23175 | 22849 | ID=Msa1452530-mRNA-1:five_prime_utr;Parent=Msa1452530-mRNA-1 |
| tig0033997 | five_prime_UTR | 17690 | 18813 | 17690 | ID=Msa1452530-mRNA-1:five_prime_utr;Parent=Msa1452530-mRNA-1 |
| tig0033997 | five_prime_UTR | 17468 | 17588 | 17468 | ID=Msa1452530-mRNA-1:five_prime_utr;Parent=Msa1452530-mRNA-1 |
| tig0033997 | five_prime_UTR | 17169 | 17342 | 17169 | ID=Msa1452530-mRNA-1:five_prime_utr;Parent=Msa1452530-mRNA-1 |
| tig0033997 | five_prime_UTR | 12047 | 12206 | 12047 | ID=Msa1452530-mRNA-1:five_prime_utr;Parent=Msa1452530-mRNA-1 |
| tig0033997 | five_prime_UTR | 11880 | 11956 | 11880 | ID=Msa1452530-mRNA-1:five_prime_utr;Parent=Msa1452530-mRNA-1 |
| tig0033997 | five_prime_UTR | 11743 | 11796 | 11743 | ID=Msa1452530-mRNA-1:five_prime_utr;Parent=Msa1452530-mRNA-1 |
| tig0033997 | five_prime_UTR | 10758 | 11432 | 10758 | ID=Msa1452530-mRNA-1:five_prime_utr;Parent=Msa1452530-mRNA-1 |
| tig0033997 | five_prime_UTR | 10359 | 10675 | 10359 | ID=Msa1452530-mRNA-1:five_prime_utr;Parent=Msa1452530-mRNA-1 |
| tig0033997 | five_prime_UTR | 10086 | 10246 | 10086 | ID=Msa1452530-mRNA-1:five_prime_utr;Parent=Msa1452530-mRNA-1 |
| tig0033997 | five_prime_UTR | 9852 | 9955 | 9852 | ID=Msa1452530-mRNA-1:five_prime_utr;Parent=Msa1452530-mRNA-1 |
| tig0033997 | five_prime_UTR | 9528 | 9759 | 9528 | ID=Msa1452530-mRNA-1:five_prime_utr;Parent=Msa1452530-mRNA-1 |
| tig0033997 | five_prime_UTR | 9304 | 9428 | 9304 | ID=Msa1452530-mRNA-1:five_prime_utr;Parent=Msa1452530-mRNA-1 |
| tig0033997 | five_prime_UTR | 8864 | 9196 | 8864 | ID=Msa1452530-mRNA-1:five_prime_utr;Parent=Msa1452530-mRNA-1 |
| tig0033997 | five_prime_UTR | 8492 | 8782 | 8492 | ID=Msa1452530-mRNA-1:five_prime_utr;Parent=Msa1452530-mRNA-1 |
| tig0033997 | five_prime_UTR | 7905 | 8382 | 7905 | ID=Msa1452530-mRNA-1:five_prime_utr;Parent=Msa1452530-mRNA-1 |
| tig0033997 | five_prime_UTR | 7489 | 7588 | 7489 | ID=Msa1452530-mRNA-1:five_prime_utr;Parent=Msa1452530-mRNA-1 |
| tig0033997 | CDS | 7456 | 7488 | 7456 | ID=Msa1452530-mRNA-1:cds;Parent=Msa1452530-mRNA-1 |
| tig0033997 | CDS | 7075 | 7329 | 7075 | ID=Msa1452530-mRNA-1:cds;Parent=Msa1452530-mRNA-1 |
| tig0033997 | CDS | 6720 | 6983 | 6720 | ID=Msa1452530-mRNA-1:cds;Parent=Msa1452530-mRNA-1 |
| tig0033997 | three_prime_UTR | 6699 | 6719 | 6699 | ID=Msa1452530-mRNA-1:three_prime_utr;Parent=Msa1452530-mRNA-1 |
| tig0033997 | three_prime_UTR | 3523 | 3731 | 3523 | ID=Msa1452530-mRNA-1:three_prime_utr;Parent=Msa1452530-mRNA-1 |
| Gene Sequence |
>Msa1452530 ATGTACTCTGCCTTACCCTATGCATTTTCACAGATTTTGGTAGAGTTACCTTATGTCTTTGCTCAAGCCGTGACATATGGAGCTATAGTCTATGCCATGATCGGATTTGACTGGACCGCAGAGAAATTCTTGTGGTATCTATTCTTCATGTACTTCACATTGCTCTACTTCACCTTCTATGGTATGATGGCTGTGGCAGTGACACCAAATCACCATGTTGCTTCTATTGTGGCTGCTGCATTTTATGCAATTTGGAATCTCTTTTCAGGATTTGTCGTCCCAAGACCTAGCATTCCAATATGGTGGAGATGGTACTATTGGGCATGTCCAGTTGCATGGACCATCTATGGATTGGTTGCATCTCAATTTGGAGATATAACTACTGTTATGTCGACCGAAGGTGGAAAAGATGTGAAAACTTTCCTTGACGACTTTTTTGGTATCCAACATGACTTCATAGGATGGTGTGCACTTGTGGTTGGTGGAATCGCAGTGGGCTTTGCCTTTATTTTCGCTGTTGCAATCAAGTCCTTTAACTTCCAAAAGAGATAG |
| Protein sequence |
>Msa1452530 MYSALPYAFSQILVELPYVFAQAVTYGAIVYAMIGFDWTAEKFLWYLFFMYFTLLYFTFYGMMAVAVTPNHHVASIVAAAFYAIWNLFSGFVVPRPSIPIWWRWYYWACPVAWTIYGLVASQFGDITTVMSTEGGKDVKTFLDDFFGIQHDFIGWCALVVGGIAVGFAFIFAVAIKSFNFQKR* |
