AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1455600

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1455600 MtrunA17_Chr3g0142501 99.622 529 2 0 1 529 1 529 0.0 1095
Msa1455600 MtrunA17_Chr8g0372031 62.330 515 191 1 14 528 23 534 0.0 638
Msa1455600 MtrunA17_Chr5g0439311 44.798 471 246 9 58 519 32 497 5.18e-138 409
Msa1455600 MtrunA17_Chr4g0038131 43.154 482 255 7 58 528 29 502 3.11e-136 408
Msa1455600 MtrunA17_Chr8g0388051 46.482 469 237 10 58 518 29 491 2.21e-135 406
Msa1455600 MtrunA17_Chr3g0119371 43.524 471 252 9 58 519 41 506 6.17e-130 389
Msa1455600 MtrunA17_Chr1g0164201 44.563 469 239 10 58 518 26 481 1.10e-126 379
Msa1455600 MtrunA17_Chr5g0397201 43.644 472 245 7 58 523 41 497 5.32e-125 375
Msa1455600 MtrunA17_Chr3g0145261 42.827 474 250 9 56 519 23 485 2.52e-122 368
Msa1455600 MtrunA17_Chr3g0119821 42.004 469 253 8 56 516 34 491 7.87e-122 367
Msa1455600 MtrunA17_Chr3g0086311 41.453 468 252 7 58 520 31 481 9.00e-119 358
Msa1455600 MtrunA17_Chr1g0195271 41.079 482 266 9 51 525 33 503 3.45e-115 350
Msa1455600 MtrunA17_Chr2g0291461 41.698 530 267 14 14 518 77 589 2.97e-114 351
Msa1455600 MtrunA17_Chr5g0444721 39.219 538 242 14 58 518 35 564 1.53e-113 348
Msa1455600 MtrunA17_Chr8g0391391 41.300 477 247 11 56 516 29 488 3.11e-113 345
Msa1455600 MtrunA17_Chr3g0080171 41.880 468 248 10 58 516 26 478 4.44e-112 341
Msa1455600 MtrunA17_Chr4g0018301 40.343 466 255 8 58 516 46 495 3.67e-110 337
Msa1455600 MtrunA17_Chr3g0120661 39.212 533 285 15 14 524 82 597 5.32e-106 330
Msa1455600 MtrunA17_Chr5g0397181 40.686 408 222 5 117 519 1 393 1.95e-104 318
Msa1455600 MtrunA17_Chr4g0018361 38.703 478 260 12 56 516 26 487 1.11e-101 315
Msa1455600 MtrunA17_Chr3g0126111 36.981 530 282 18 18 519 76 581 2.95e-95 301
Msa1455600 MtrunA17_Chr3g0136381 40.385 364 199 5 58 420 31 377 1.85e-91 286
Msa1455600 MtrunA17_Chr4g0018341 39.024 287 155 7 236 516 2 274 1.35e-58 196
Msa1455600 MtrunA17_Chr4g0018351 43.085 188 104 2 53 239 26 211 2.81e-44 155
Msa1455600 MtrunA17_Chr1g0164181 43.889 180 97 1 98 273 3 182 4.85e-41 150
Msa1455600 MtrunA17_Chr1g0164181 40.476 168 93 4 354 516 164 329 5.71e-35 134
Msa1455600 MtrunA17_Chr4g0072811 44.586 157 86 1 373 528 19 175 2.80e-38 139
Msa1455600 MtrunA17_Chr7g0269571 35.227 176 98 3 366 526 43 217 2.35e-31 121
Msa1455600 MtrunA17_Chr3g0136371 47.778 90 43 2 421 506 2 91 3.76e-21 88.6
Msa1455600 MtrunA17_Chr4g0022271 37.405 131 71 4 375 500 29 153 6.09e-20 87.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1455600 AT1G19940.2 68.371 528 154 4 1 528 1 515 0.0 709
Msa1455600 AT1G19940.1 68.371 528 154 4 1 528 1 515 0.0 709
Msa1455600 AT1G75680.1 69.187 529 157 5 1 528 1 524 0.0 708
Msa1455600 AT1G19940.3 65.952 420 130 4 1 420 1 407 0.0 546
Msa1455600 AT4G11050.2 45.798 476 244 9 58 525 27 496 1.65e-139 414
Msa1455600 AT4G11050.3 46.008 476 243 10 58 525 27 496 9.95e-139 415
Msa1455600 AT4G11050.1 46.008 476 243 10 58 525 27 496 1.11e-138 415
Msa1455600 AT1G64390.1 44.676 479 251 9 58 528 26 498 1.54e-136 409
Msa1455600 AT2G32990.1 43.644 472 244 10 58 516 40 502 3.82e-131 392
Msa1455600 AT2G44540.1 44.397 473 247 7 52 518 28 490 1.71e-128 384
Msa1455600 AT2G44550.1 45.435 471 238 9 53 516 29 487 3.03e-128 383
Msa1455600 AT2G44560.1 45.032 473 244 8 52 518 28 490 4.74e-126 378
Msa1455600 AT1G48930.1 46.055 469 235 7 58 516 31 491 4.04e-125 380
Msa1455600 AT2G44570.1 42.887 471 252 9 55 518 31 491 3.85e-122 368
Msa1455600 AT4G23560.1 42.463 471 251 6 57 522 23 478 2.19e-119 360
Msa1455600 AT1G71380.1 42.706 473 248 9 52 516 19 476 1.15e-118 358
Msa1455600 AT4G02290.1 41.667 468 255 9 56 516 51 507 1.15e-118 360
Msa1455600 AT1G02800.1 41.983 474 255 10 55 521 41 501 6.30e-118 357
Msa1455600 AT3G43860.2 41.845 466 249 7 58 518 35 483 3.12e-117 355
Msa1455600 AT3G43860.1 41.845 466 249 7 58 518 35 483 3.12e-117 355
Msa1455600 AT1G22880.1 42.616 474 249 9 58 523 25 483 5.19e-117 354
Msa1455600 AT4G09740.1 42.340 470 251 7 58 522 24 478 6.86e-117 353
Msa1455600 AT4G39010.1 40.764 471 259 9 56 516 28 488 6.66e-115 349
Msa1455600 AT1G23210.1 40.000 470 254 9 58 515 29 482 5.90e-114 347
Msa1455600 AT1G70710.1 40.127 471 252 9 58 515 29 482 6.75e-114 347
Msa1455600 AT4G39000.1 39.496 476 268 9 51 516 19 484 6.04e-111 339
Msa1455600 AT5G49720.1 40.566 530 273 14 14 518 74 586 3.83e-108 336
Msa1455600 AT4G24260.1 43.149 489 244 14 58 522 109 587 9.64e-105 327
Msa1455600 AT4G38990.2 37.654 486 268 12 51 516 17 487 1.92e-100 312
Msa1455600 AT1G65610.1 36.484 529 298 13 14 521 82 593 3.20e-100 315
Msa1455600 AT1G22880.2 41.546 414 220 8 117 523 1 399 3.65e-99 305
Msa1455600 AT4G02290.2 40.148 406 226 8 117 516 1 395 1.44e-95 296
Msa1455600 AT2G44570.2 41.514 383 212 7 55 435 31 403 6.58e-89 279
Msa1455600 AT4G39010.2 39.516 372 209 8 56 421 28 389 9.00e-83 263
Msa1455600 AT1G22880.3 44.728 313 155 7 58 366 25 323 8.85e-71 230
Msa1455600 AT4G24260.3 42.119 387 193 13 58 420 109 488 8.10e-70 232
Msa1455600 AT4G24260.2 44.164 317 150 11 58 352 109 420 1.67e-63 214
Msa1455600 AT4G38990.1 36.311 347 183 7 51 392 17 330 1.47e-59 201

Find 180 sgRNAs with CRISPR-Local

Find 301 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAACAGAGTTTGGGGTTT+TGG 0.147944 tig0036379:-8489 None:intergenic
CATCACGTATTATTAGTTTA+TGG 0.157518 tig0036379:+11725 Msa1455600:three_prime_UTR
GTTTATTGCGTTTGTGATTC+TGG 0.168400 tig0036379:+8584 Msa1455600:CDS
GAGCATAATCTTCTCCATTT+TGG 0.188257 tig0036379:-9880 None:intergenic
GGAGAAGATTATGCTCAATT+TGG 0.211354 tig0036379:+9887 Msa1455600:CDS
TATTATAACTCAACTGGATA+TGG 0.212670 tig0036379:+9782 Msa1455600:CDS
TAATAACACATTTCTTATTT+AGG 0.225442 tig0036379:+8371 Msa1455600:five_prime_UTR
TTTATATAGATGGTCTGATA+TGG 0.236900 tig0036379:+10740 Msa1455600:intron
ACAACGATAATGTCTATATT+TGG 0.241703 tig0036379:-8338 None:intergenic
ATGAGACTTACATTGATTCT+AGG 0.242483 tig0036379:+11511 Msa1455600:CDS
GTACTTCCTTGTAAAATATA+TGG 0.249625 tig0036379:+11879 Msa1455600:three_prime_UTR
TCCCCATTTCTCGCCGCAAC+CGG 0.259382 tig0036379:+8172 Msa1455600:five_prime_UTR
TTGAGGGGTATAATGGTAAA+AGG 0.271920 tig0036379:-8000 None:intergenic
TAATGTCTATATTTGGAAAT+AGG 0.284072 tig0036379:-8331 None:intergenic
CAAATGCAGAAAACAGAGTT+TGG 0.299311 tig0036379:-8497 None:intergenic
GGAGAATGTTCTGTATATTC+AGG 0.309453 tig0036379:+9373 Msa1455600:CDS
TCGTGGGCAATTCTAGAATA+TGG 0.312428 tig0036379:+9263 Msa1455600:CDS
AGCAATGGCATCAGCATCTT+TGG 0.319777 tig0036379:+9640 Msa1455600:CDS
AGTTAGCAATGTATTTGTTT+GGG 0.320297 tig0036379:+8412 Msa1455600:five_prime_UTR
GGAGGACCGGGAACCGGTTC+GGG 0.321906 tig0036379:-8672 None:intergenic
TTTGTGGCTGTTGGAGAGTC+TGG 0.335910 tig0036379:-10385 None:intergenic
GAGAGGGGATTCGGCTCTTA+AGG 0.338315 tig0036379:+9142 Msa1455600:CDS
CAGTATGTGACTGGCCAAAA+TGG 0.350134 tig0036379:+9866 Msa1455600:CDS
GCCGGGGATCACATGAAGTT+TGG 0.352977 tig0036379:+9209 Msa1455600:CDS
CTTTCAAGTTCATATGGTTC+CGG 0.354604 tig0036379:+10313 Msa1455600:CDS
GACCCGGTTGCGGCGAGAAA+TGG 0.355828 tig0036379:-8175 None:intergenic
GAGTTAGCAATGTATTTGTT+TGG 0.360242 tig0036379:+8411 Msa1455600:five_prime_UTR
GATAAACCCGAACCGGTTCC+CGG 0.365719 tig0036379:+8666 Msa1455600:CDS
GTGTTGGAGGAAAATGGATA+TGG 0.365793 tig0036379:-8207 None:intergenic
TTATAATATGTCGCAACTTC+AGG 0.368599 tig0036379:-9767 None:intergenic
GGAGCTACATACTATAGTTT+AGG 0.374553 tig0036379:-11634 None:intergenic
TGTCCCAACTGAACCAGGTT+GGG 0.383206 tig0036379:-9913 None:intergenic
AGGAGGACCGGGAACCGGTT+CGG 0.385546 tig0036379:-8673 None:intergenic
GTCGTTGTGTTGGAGGAAAA+TGG 0.386601 tig0036379:-8213 None:intergenic
TTCTCATATCCAACTTCATA+AGG 0.387488 tig0036379:+8050 Msa1455600:five_prime_UTR
GCTAGGTTGTTGGTTGGTGC+TGG 0.390689 tig0036379:+8247 Msa1455600:five_prime_UTR
ACCCGGTTGCGGCGAGAAAT+GGG 0.395938 tig0036379:-8174 None:intergenic
AAATGCAGAAAACAGAGTTT+GGG 0.396742 tig0036379:-8496 None:intergenic
AGTCATTAATTAATGTATAC+AGG 0.400302 tig0036379:+11806 Msa1455600:three_prime_UTR
ATTATAACTCAACTGGATAT+GGG 0.400764 tig0036379:+9783 Msa1455600:CDS
AATTGTTTGAAATCGTTAAA+CGG 0.407563 tig0036379:-8275 None:intergenic
ACTAATAATACGTGATGATA+AGG 0.416229 tig0036379:-11719 None:intergenic
AAGGGCCTTTCAAGTTCATA+TGG 0.416309 tig0036379:+10307 Msa1455600:CDS
TTTCTCGCCGCAACCGGGTC+GGG 0.419432 tig0036379:+8178 Msa1455600:five_prime_UTR
TCTCGTCAATGCTCATCCTT+CGG 0.427984 tig0036379:+9352 Msa1455600:CDS
AATAAGTTGGAGAGGGGATT+CGG 0.430660 tig0036379:+9133 Msa1455600:CDS
AATGGATGCTGTTGGCCAAT+TGG 0.431030 tig0036379:+9292 Msa1455600:CDS
TTCCAGGCTGATTACGTGTT+GGG 0.431458 tig0036379:+11300 Msa1455600:intron
TTCAGCTGCAACATCTGATC+CGG 0.435202 tig0036379:-9609 None:intergenic
ATCAATGGCACCAGGAGGAC+CGG 0.439759 tig0036379:-8685 None:intergenic
GCGACATATTATAACTCAAC+TGG 0.441782 tig0036379:+9776 Msa1455600:CDS
TGAGTGTGAACCGTGGTGTC+TGG 0.442330 tig0036379:+11776 Msa1455600:three_prime_UTR
CCGAATCCCAATGTAGCTAC+TGG 0.444550 tig0036379:+11462 Msa1455600:CDS
GTTGCAAGGATGGGTGGCAG+TGG 0.446186 tig0036379:+11424 Msa1455600:CDS
TCTAGAATATGGAGATCAAA+TGG 0.446875 tig0036379:+9274 Msa1455600:CDS
ATCTAGAGTAAGCTTCTTCA+AGG 0.446995 tig0036379:+10282 Msa1455600:CDS
AGCTGAAACTGCAGCAGCAA+TGG 0.448020 tig0036379:+9625 Msa1455600:CDS
CCCCATTTCTCGCCGCAACC+GGG 0.448967 tig0036379:+8173 Msa1455600:five_prime_UTR
AAGTTTAGTCACCACATCTT+CGG 0.449573 tig0036379:+11596 Msa1455600:CDS
GCACCAGGAGGACCGGGAAC+CGG 0.455702 tig0036379:-8678 None:intergenic
TCGAGACTTTGCCAGATCTC+AGG 0.456961 tig0036379:+10894 Msa1455600:CDS
TTATATAGATGGTCTGATAT+GGG 0.457104 tig0036379:+10741 Msa1455600:intron
AGTTGGGACAACAAGCTTGC+CGG 0.460646 tig0036379:+9926 Msa1455600:CDS
CAACACTTGTGATCTGCAAC+AGG 0.466398 tig0036379:-9527 None:intergenic
CTCTGTGATGCACAAATTGT+GGG 0.469293 tig0036379:-11371 None:intergenic
AATCTTGTGCTGGTTCCAAT+TGG 0.470000 tig0036379:-9307 None:intergenic
TAGAGAACAACAAAATAAGT+TGG 0.470544 tig0036379:+9120 Msa1455600:CDS
CCAGTAGCTACATTGGGATT+CGG 0.470664 tig0036379:-11462 None:intergenic
CTCAATTTGGAAGCCCAACC+TGG 0.472205 tig0036379:+9900 Msa1455600:CDS
GTCTGATATGGGTGAGTGAA+TGG 0.473028 tig0036379:+10752 Msa1455600:CDS
GCTAAGAATGCAGATGCAAC+AGG 0.473355 tig0036379:-10790 None:intergenic
ATGAATGCATACCTGAGATC+TGG 0.474526 tig0036379:-10905 None:intergenic
ACAGATCAATAACGTGATTA+CGG 0.475142 tig0036379:-11938 None:intergenic
CTTGTTGTCCCAACTGAACC+AGG 0.475271 tig0036379:-9918 None:intergenic
GGAAATGTCCCTTATGAAGT+TGG 0.475379 tig0036379:-8059 None:intergenic
ATTTCTCGCCGCAACCGGGT+CGG 0.478416 tig0036379:+8177 Msa1455600:five_prime_UTR
AGGATACGTTCACTTGTAAG+AGG 0.479664 tig0036379:-9583 None:intergenic
ATACTCTGTCGTTGTGTTGG+AGG 0.482880 tig0036379:-8220 None:intergenic
CAAAAGGAGGATGTTGTGGT+TGG 0.485199 tig0036379:+8562 Msa1455600:CDS
TCAATGCAGTTTCAGATCGA+TGG 0.485525 tig0036379:-8300 None:intergenic
GAACCGGTTCCCGGTCCTCC+TGG 0.489943 tig0036379:+8675 Msa1455600:CDS
ATGGGGAGAAGGAGTCGAAA+TGG 0.490979 tig0036379:-8156 None:intergenic
GGTCTGACCCGACCCGGTTG+CGG 0.492298 tig0036379:-8185 None:intergenic
ACCTACTTACTTCTAAATCT+AGG 0.493860 tig0036379:+8102 Msa1455600:five_prime_UTR
GTAGCTACTGGAGCACTTGT+TGG 0.494083 tig0036379:+11474 Msa1455600:CDS
GTAAGCTTCTTCAAGGCAAA+GGG 0.494576 tig0036379:+10289 Msa1455600:CDS
AGTATACTCTGTCGTTGTGT+TGG 0.494988 tig0036379:-8223 None:intergenic
AACAACAAAATAAGTTGGAG+AGG 0.494998 tig0036379:+9125 Msa1455600:CDS
AAATTCAGATAAACCCGAAC+CGG 0.495107 tig0036379:+8659 Msa1455600:CDS
CAAAAGGAATGTATGATGCC+GGG 0.495516 tig0036379:+9192 Msa1455600:CDS
GCAGATTTAGATCTAACAAA+AGG 0.496053 tig0036379:+9176 Msa1455600:CDS
TCGAAGATCTGATGGTTTGA+AGG 0.496987 tig0036379:-10875 None:intergenic
CCTCTGTGATGCACAAATTG+TGG 0.501280 tig0036379:-11372 None:intergenic
GAAGCCCAACCTGGTTCAGT+TGG 0.502198 tig0036379:+9909 Msa1455600:CDS
GTGATTCTGGCTCTTGTGGT+CGG 0.505371 tig0036379:+8597 Msa1455600:CDS
AAGCCCAACCTGGTTCAGTT+GGG 0.506162 tig0036379:+9910 Msa1455600:CDS
ACAAAAGGAATGTATGATGC+CGG 0.506505 tig0036379:+9191 Msa1455600:CDS
TATAACAGTGCTGTCATAGT+TGG 0.509805 tig0036379:+11564 Msa1455600:CDS
ATACTAGCTAGGTTGTTGGT+TGG 0.510801 tig0036379:+8241 Msa1455600:five_prime_UTR
TGCAACATCTGATCCGGGAC+AGG 0.511750 tig0036379:-9603 None:intergenic
GAGTATACTAGCTAGGTTGT+TGG 0.514068 tig0036379:+8237 Msa1455600:five_prime_UTR
GCTACTGGAGCACTTGTTGG+TGG 0.515300 tig0036379:+11477 Msa1455600:CDS
ACCTGTAGCATGGTAGAGCC+AGG 0.516769 tig0036379:-9828 None:intergenic
GTTAGCAATGTATTTGTTTG+GGG 0.523868 tig0036379:+8413 Msa1455600:five_prime_UTR
TTGTCCCAACTGAACCAGGT+TGG 0.523981 tig0036379:-9914 None:intergenic
AACCAGCACAAGATTCTCTC+AGG 0.525412 tig0036379:+9315 Msa1455600:CDS
TACTGCCTCAGTGCTATCGT+GGG 0.526117 tig0036379:+9247 Msa1455600:CDS
TAGTTTAGGAGAAGGATTGA+AGG 0.527196 tig0036379:-11620 None:intergenic
AGCTCTTCATACCTGAGTTC+CGG 0.531252 tig0036379:-9945 None:intergenic
CACCTGAGAGAATCTTGTGC+TGG 0.533013 tig0036379:-9317 None:intergenic
TCTCATATCCAACTTCATAA+GGG 0.534299 tig0036379:+8051 Msa1455600:five_prime_UTR
AGTGCTCCAGTAGCTACATT+GGG 0.536888 tig0036379:-11468 None:intergenic
CTCATGCTAGCTTCGTCGGT+GGG 0.537380 tig0036379:-7959 None:intergenic
CAACAAGCTTGCCGGAACTC+AGG 0.539769 tig0036379:+9934 Msa1455600:CDS
TCCTACCTTCAGTATGTGAC+TGG 0.540197 tig0036379:+9857 Msa1455600:CDS
CTTCCAGGCTGATTACGTGT+TGG 0.540229 tig0036379:+11299 Msa1455600:intron
CGGAACCATATGAACTTGAA+AGG 0.540605 tig0036379:-10312 None:intergenic
AAGTGCTCCAGTAGCTACAT+TGG 0.543749 tig0036379:-11469 None:intergenic
GCAAAGTCTCGAAGATCTGA+TGG 0.544007 tig0036379:-10883 None:intergenic
ATGTAAGTCTCATTTAAGAA+AGG 0.549620 tig0036379:-11501 None:intergenic
GTTTGTGATTCTGGCTCTTG+TGG 0.550904 tig0036379:+8593 Msa1455600:CDS
TTGACTCATGCTAGCTTCGT+CGG 0.552816 tig0036379:-7963 None:intergenic
AGTAAGCTTCTTCAAGGCAA+AGG 0.552883 tig0036379:+10288 Msa1455600:CDS
ACCTGGCTCTACCATGCTAC+AGG 0.553331 tig0036379:+9827 Msa1455600:CDS
ACCAAACTTCATGTGATCCC+CGG 0.556653 tig0036379:-9210 None:intergenic
CAATAGAATAAGCTCAGAGC+TGG 0.556969 tig0036379:-11857 None:intergenic
CAAGTGAACGTATCCTGTCC+CGG 0.560701 tig0036379:+9590 Msa1455600:CDS
GGATTGAAGGGCCGAAGATG+TGG 0.569561 tig0036379:-11607 None:intergenic
GTGAACCGTGGTGTCTGGAT+AGG 0.571683 tig0036379:+11781 Msa1455600:three_prime_UTR
CTGTTGCAGATCACAAGTGT+TGG 0.571688 tig0036379:+9528 Msa1455600:CDS
TCAATGGCACCAGGAGGACC+GGG 0.573055 tig0036379:-8684 None:intergenic
ACAGCACTGTTATATGTGCT+TGG 0.573334 tig0036379:-11555 None:intergenic
GGGGATTCGGCTCTTAAGGA+TGG 0.573397 tig0036379:+9146 Msa1455600:CDS
TGCGGCGAGAAATGGGGAGA+AGG 0.575232 tig0036379:-8167 None:intergenic
TTACTGCCTCAGTGCTATCG+TGG 0.577110 tig0036379:+9246 Msa1455600:CDS
AGTTTAGGAGAAGGATTGAA+GGG 0.578741 tig0036379:-11619 None:intergenic
ACATACTATAGTTTAGGAGA+AGG 0.578891 tig0036379:-11628 None:intergenic
TTATAACTCAACTGGATATG+GGG 0.582247 tig0036379:+9784 Msa1455600:CDS
AAGACCACACATTACAGCCT+CGG 0.582653 tig0036379:-10359 None:intergenic
ACAACAAAATAAGTTGGAGA+GGG 0.586545 tig0036379:+9126 Msa1455600:CDS
AGCTACATTGGGATTCGGAT+CGG 0.590300 tig0036379:-11457 None:intergenic
TCTAGGAACAATTCTATGCA+AGG 0.591369 tig0036379:+11528 Msa1455600:CDS
TCAGCTGCAACATCTGATCC+GGG 0.591508 tig0036379:-9608 None:intergenic
GATATGGGTCTGACCCGACC+CGG 0.592804 tig0036379:-8191 None:intergenic
TGTTGGAGGAAAATGGATAT+GGG 0.596187 tig0036379:-8206 None:intergenic
CGTTCCTCAGTAATTAACTC+AGG 0.597205 tig0036379:-9557 None:intergenic
GCCTAGATTTAGAAGTAAGT+AGG 0.598675 tig0036379:-8103 None:intergenic
AGATCAAAAGGAGGATGTTG+TGG 0.599433 tig0036379:+8558 Msa1455600:CDS
CCCGGTTGCGGCGAGAAATG+GGG 0.601279 tig0036379:-8173 None:intergenic
GTAATCACGTTATTGATCTG+TGG 0.604164 tig0036379:+11940 Msa1455600:three_prime_UTR
ACTCATGCTAGCTTCGTCGG+TGG 0.611279 tig0036379:-7960 None:intergenic
ACTGAGGCAGTAAAAGCCAT+AGG 0.613713 tig0036379:-9236 None:intergenic
CAGCACAAGATTCTCTCAGG+TGG 0.615600 tig0036379:+9318 Msa1455600:CDS
TTTGGCCAGTCACATACTGA+AGG 0.616951 tig0036379:-9862 None:intergenic
AGGAATCATCACCTGTAGCA+TGG 0.618916 tig0036379:-9838 None:intergenic
AATGCAGAAAACAGAGTTTG+GGG 0.620773 tig0036379:-8495 None:intergenic
AAACTGGTTGCAAGGATGGG+TGG 0.621140 tig0036379:+11418 Msa1455600:CDS
TATTACTGCATGTAGAATCA+CGG 0.626929 tig0036379:-8080 None:intergenic
AAAAGGAATGTATGATGCCG+GGG 0.628087 tig0036379:+9193 Msa1455600:CDS
ATGGTAATGAACGATATCGC+GGG 0.628262 tig0036379:-8137 None:intergenic
AACGACAGAGTATACTAGCT+AGG 0.630471 tig0036379:+8230 Msa1455600:five_prime_UTR
GGATACGTTCACTTGTAAGA+GGG 0.631666 tig0036379:-9582 None:intergenic
GAATAAGCTCAGAGCTGGTG+AGG 0.639588 tig0036379:-11852 None:intergenic
GGAGATCAAATGGATGCTGT+TGG 0.641613 tig0036379:+9284 Msa1455600:CDS
TCCGCCGAGGCTGTAATGTG+TGG 0.641659 tig0036379:+10355 Msa1455600:CDS
ACCAGAAAACTCATATGCTG+AGG 0.647665 tig0036379:-11330 None:intergenic
CAACAAAATAAGTTGGAGAG+GGG 0.657191 tig0036379:+9127 Msa1455600:CDS
ATATACAGAACATTCTCCGA+AGG 0.657437 tig0036379:-9368 None:intergenic
GCCAGTCACATACTGAAGGT+AGG 0.663540 tig0036379:-9858 None:intergenic
CCACAATTTGTGCATCACAG+AGG 0.673056 tig0036379:+11372 Msa1455600:CDS
ACCACACATTACAGCCTCGG+CGG 0.674391 tig0036379:-10356 None:intergenic
GATACGTTCACTTGTAAGAG+GGG 0.676663 tig0036379:-9581 None:intergenic
AATGGTAATGAACGATATCG+CGG 0.691941 tig0036379:-8138 None:intergenic
TAGACCTGAGTTAATTACTG+AGG 0.715470 tig0036379:+9553 Msa1455600:CDS
ACTCAAAAGAAGTTGCGACG+CGG 0.717202 tig0036379:+11669 Msa1455600:three_prime_UTR
AAATTATAGAAAATCCGCCG+AGG 0.730261 tig0036379:+10342 Msa1455600:CDS
ACTAACCTATCCAGACACCA+CGG 0.763242 tig0036379:-11786 None:intergenic
AATTGCCCACGATAGCACTG+AGG 0.772045 tig0036379:-9252 None:intergenic
TGAAGTTGGATATGAGAACG+TGG 0.785477 tig0036379:-8045 None:intergenic
AATATATTGAGTGTGAACCG+TGG 0.788543 tig0036379:+11769 Msa1455600:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATAAAAAAAAACACTT+CGG - tig0036379:10052-10071 None:intergenic 10.0%
!! TAGAAATATATTACTAAAAA+TGG - tig0036379:10684-10703 None:intergenic 10.0%
!! TATAAAACATTTAATGTTAT+TGG - tig0036379:11080-11099 None:intergenic 10.0%
!!! TAATAACACATTTCTTATTT+AGG + tig0036379:8371-8390 Msa1455600:five_prime_UTR 15.0%
!!! TTCTTTTTCTTTTATATAGA+TGG + tig0036379:10730-10749 Msa1455600:intron 15.0%
!! GTAAATTCTTCTTATATTAA+GGG - tig0036379:10959-10978 None:intergenic 15.0%
!! TTAATTAGTATAAAATTGAG+TGG + tig0036379:11037-11056 Msa1455600:intron 15.0%
!!! TTTAATGTTATTGGTTTAAT+TGG - tig0036379:11071-11090 None:intergenic 15.0%
!! TTTAGTATAAATTTACATGT+AGG - tig0036379:11103-11122 None:intergenic 15.0%
!! ATTTATCTGACATTAAATAA+AGG + tig0036379:11124-11143 Msa1455600:intron 15.0%
!! TTTATCTGACATTAAATAAA+GGG + tig0036379:11125-11144 Msa1455600:intron 15.0%
!!! AATTGTTTGAAATCGTTAAA+CGG - tig0036379:8278-8297 None:intergenic 20.0%
!! TAATGTCTATATTTGGAAAT+AGG - tig0036379:8334-8353 None:intergenic 20.0%
!!! ATAATCCTTTTTTTCATTCT+CGG + tig0036379:8766-8785 Msa1455600:intron 20.0%
!!! AAAACAGTTATTTACTTTTG+CGG + tig0036379:9712-9731 Msa1455600:CDS 20.0%
!!! AATAAGTAGAAGTATCATTT+AGG - tig0036379:10138-10157 None:intergenic 20.0%
!! CTAATCAGATTATAAGTTAA+GGG - tig0036379:10609-10628 None:intergenic 20.0%
!!! AATAGTTTTATTAGATAGTC+TGG + tig0036379:10636-10655 Msa1455600:intron 20.0%
!!! TCTGAATCACATTTTATATT+GGG - tig0036379:10853-10872 None:intergenic 20.0%
!!! ATCTGAATCACATTTTATAT+TGG - tig0036379:10854-10873 None:intergenic 20.0%
!! GGTAAATTCTTCTTATATTA+AGG - tig0036379:10960-10979 None:intergenic 20.0%
!!! GGAGTTGTATTTTTTTTTTT+TGG + tig0036379:10995-11014 Msa1455600:intron 20.0%
!! AGTCATTAATTAATGTATAC+AGG + tig0036379:11806-11825 Msa1455600:three_prime_UTR 20.0%
!!! AATCAACCATATATTTTACA+AGG - tig0036379:11888-11907 None:intergenic 20.0%
!!! ATTCTTTTTGTTATTGTTGT+TGG + tig0036379:11905-11924 Msa1455600:three_prime_UTR 20.0%
! ACAACGATAATGTCTATATT+TGG - tig0036379:8341-8360 None:intergenic 25.0%
! AGTTAGCAATGTATTTGTTT+GGG + tig0036379:8412-8431 Msa1455600:five_prime_UTR 25.0%
!!! AGAAGCATATTTTTGATCAA+TGG - tig0036379:8703-8722 None:intergenic 25.0%
! TTGTGAAATGCAAGAAATAA+AGG - tig0036379:8795-8814 None:intergenic 25.0%
!!! TAATGTTTTAAAAACCGAAC+TGG + tig0036379:8886-8905 Msa1455600:intron 25.0%
!!! TGGTTTTGAAAACATTGTTT+TGG + tig0036379:9025-9044 Msa1455600:intron 25.0%
! TAGAGAACAACAAAATAAGT+TGG + tig0036379:9120-9139 Msa1455600:CDS 25.0%
! TTCATTTATCAAAATCGATG+TGG + tig0036379:9492-9511 Msa1455600:intron 25.0%
! TATTATAACTCAACTGGATA+TGG + tig0036379:9782-9801 Msa1455600:CDS 25.0%
! ATTATAACTCAACTGGATAT+GGG + tig0036379:9783-9802 Msa1455600:CDS 25.0%
! TAGAATTTGGAAACAAGATT+CGG - tig0036379:9972-9991 None:intergenic 25.0%
! TTGTTTCCAAATTCTAAACT+AGG + tig0036379:9976-9995 Msa1455600:intron 25.0%
!! AATGATCCTAGTTTAGAATT+TGG - tig0036379:9985-10004 None:intergenic 25.0%
! AATGCTTTCATGTATATACT+AGG + tig0036379:10174-10193 Msa1455600:intron 25.0%
! ATGCTTTCATGTATATACTA+GGG + tig0036379:10175-10194 Msa1455600:intron 25.0%
!! TCTGACTTGATATTAACTAA+TGG + tig0036379:10444-10463 Msa1455600:intron 25.0%
!! CTGACTTGATATTAACTAAT+GGG + tig0036379:10445-10464 Msa1455600:intron 25.0%
! AATTTGTGTATTCAATGCAT+TGG + tig0036379:10505-10524 Msa1455600:intron 25.0%
! GCTAATCAGATTATAAGTTA+AGG - tig0036379:10610-10629 None:intergenic 25.0%
! TTTATATAGATGGTCTGATA+TGG + tig0036379:10740-10759 Msa1455600:intron 25.0%
! TTATATAGATGGTCTGATAT+GGG + tig0036379:10741-10760 Msa1455600:intron 25.0%
!!! TTTTTTTTTGGTACAGATCA+AGG + tig0036379:11007-11026 Msa1455600:intron 25.0%
!!! AAGAAATTGTGCGTATTTTT+TGG - tig0036379:11213-11232 None:intergenic 25.0%
! TACGCACAATTTCTTATAAT+AGG + tig0036379:11218-11237 Msa1455600:intron 25.0%
! ACGCACAATTTCTTATAATA+GGG + tig0036379:11219-11238 Msa1455600:intron 25.0%
! ATGTAAGTCTCATTTAAGAA+AGG - tig0036379:11504-11523 None:intergenic 25.0%
! ACTAATAATACGTGATGATA+AGG - tig0036379:11722-11741 None:intergenic 25.0%
!! CATCACGTATTATTAGTTTA+TGG + tig0036379:11725-11744 Msa1455600:three_prime_UTR 25.0%
! GTACTTCCTTGTAAAATATA+TGG + tig0036379:11879-11898 Msa1455600:three_prime_UTR 25.0%
TTCTCATATCCAACTTCATA+AGG + tig0036379:8050-8069 Msa1455600:five_prime_UTR 30.0%
TCTCATATCCAACTTCATAA+GGG + tig0036379:8051-8070 Msa1455600:five_prime_UTR 30.0%
TATTACTGCATGTAGAATCA+CGG - tig0036379:8083-8102 None:intergenic 30.0%
ACCTACTTACTTCTAAATCT+AGG + tig0036379:8102-8121 Msa1455600:five_prime_UTR 30.0%
GAGTTAGCAATGTATTTGTT+TGG + tig0036379:8411-8430 Msa1455600:five_prime_UTR 30.0%
GTTAGCAATGTATTTGTTTG+GGG + tig0036379:8413-8432 Msa1455600:five_prime_UTR 30.0%
AAATGCAGAAAACAGAGTTT+GGG - tig0036379:8499-8518 None:intergenic 30.0%
AGAGAAAAATCGAGATCAAA+AGG + tig0036379:8546-8565 Msa1455600:CDS 30.0%
ATTTCTTGCATTTCACAATG+TGG + tig0036379:8797-8816 Msa1455600:intron 30.0%
!! CAATGTGGTTGGTTTATTAT+AGG + tig0036379:8812-8831 Msa1455600:intron 30.0%
!! AATACTTTGGTGGGTTATTT+AGG - tig0036379:8981-9000 None:intergenic 30.0%
! AACAACAAAATAAGTTGGAG+AGG + tig0036379:9125-9144 Msa1455600:CDS 30.0%
! ACAACAAAATAAGTTGGAGA+GGG + tig0036379:9126-9145 Msa1455600:CDS 30.0%
GCAGATTTAGATCTAACAAA+AGG + tig0036379:9176-9195 Msa1455600:CDS 30.0%
! TCTAGAATATGGAGATCAAA+TGG + tig0036379:9274-9293 Msa1455600:CDS 30.0%
! TTCTGTATATTCAGGTTAGT+TGG + tig0036379:9381-9400 Msa1455600:intron 30.0%
TTATAATATGTCGCAACTTC+AGG - tig0036379:9770-9789 None:intergenic 30.0%
TTATAACTCAACTGGATATG+GGG + tig0036379:9784-9803 Msa1455600:CDS 30.0%
! TAGGATCATTTTATAGCTGA+AGG + tig0036379:9995-10014 Msa1455600:intron 30.0%
GTCTATTCTATGTAATGTGT+AGG - tig0036379:10087-10106 None:intergenic 30.0%
TAGGATAAGATAATGCCATA+AGG - tig0036379:10119-10138 None:intergenic 30.0%
TGCTTTCATGTATATACTAG+GGG + tig0036379:10176-10195 Msa1455600:intron 30.0%
ATAATCCTTCTTCTTAATGC+AGG + tig0036379:10252-10271 Msa1455600:intron 30.0%
!! TTTTCTATAATTTTGGAGGC+CGG - tig0036379:10335-10354 None:intergenic 30.0%
! CGGATTTTCTATAATTTTGG+AGG - tig0036379:10339-10358 None:intergenic 30.0%
!!! AATTTTCATAAAAAGTGCCC+CGG - tig0036379:10489-10508 None:intergenic 30.0%
!!! AGTTTTATTAGATAGTCTGG+AGG + tig0036379:10639-10658 Msa1455600:intron 30.0%
! TTTTTGTAGAATACTCTCTC+TGG + tig0036379:10933-10952 Msa1455600:intron 30.0%
TTTACCAAAATCACAGATCA+AGG + tig0036379:10974-10993 Msa1455600:intron 30.0%
AAAACTTGTCTAATACTTCC+AGG + tig0036379:11284-11303 Msa1455600:intron 30.0%
!! ATGAGACTTACATTGATTCT+AGG + tig0036379:11511-11530 Msa1455600:CDS 30.0%
ACATACTATAGTTTAGGAGA+AGG - tig0036379:11631-11650 None:intergenic 30.0%
!! CTTTTGAGTGGAATTGAATA+TGG - tig0036379:11658-11677 None:intergenic 30.0%
ACAGATCAATAACGTGATTA+CGG - tig0036379:11941-11960 None:intergenic 30.0%
! TTGAGGGGTATAATGGTAAA+AGG - tig0036379:8003-8022 None:intergenic 35.0%
GCCTAGATTTAGAAGTAAGT+AGG - tig0036379:8106-8125 None:intergenic 35.0%
! AATGGTAATGAACGATATCG+CGG - tig0036379:8141-8160 None:intergenic 35.0%
TGTTGGAGGAAAATGGATAT+GGG - tig0036379:8209-8228 None:intergenic 35.0%
AATGCAGAAAACAGAGTTTG+GGG - tig0036379:8498-8517 None:intergenic 35.0%
CAAATGCAGAAAACAGAGTT+TGG - tig0036379:8500-8519 None:intergenic 35.0%
GAAAAATCGAGATCAAAAGG+AGG + tig0036379:8549-8568 Msa1455600:CDS 35.0%
GTTTATTGCGTTTGTGATTC+TGG + tig0036379:8584-8603 Msa1455600:CDS 35.0%
AAATTCAGATAAACCCGAAC+CGG + tig0036379:8659-8678 Msa1455600:CDS 35.0%
!! TATTTTTGATCAATGGCACC+AGG - tig0036379:8696-8715 None:intergenic 35.0%
GGTAACCGAGAATGAAAAAA+AGG - tig0036379:8774-8793 None:intergenic 35.0%
AACCGAACTGGATATTGAAT+CGG + tig0036379:8898-8917 Msa1455600:intron 35.0%
TACGGTTCAAATACTTTGGT+GGG - tig0036379:8990-9009 None:intergenic 35.0%
ATGCTACGGTTCAAATACTT+TGG - tig0036379:8994-9013 None:intergenic 35.0%
CCAAATCAAATCACATGCTA+CGG - tig0036379:9008-9027 None:intergenic 35.0%
! CAACAAAATAAGTTGGAGAG+GGG + tig0036379:9127-9146 Msa1455600:CDS 35.0%
ACAAAAGGAATGTATGATGC+CGG + tig0036379:9191-9210 Msa1455600:CDS 35.0%
!!! CATGAAGTTTGGTTTTCCTA+TGG + tig0036379:9220-9239 Msa1455600:CDS 35.0%
ATATACAGAACATTCTCCGA+AGG - tig0036379:9371-9390 None:intergenic 35.0%
GGAGAATGTTCTGTATATTC+AGG + tig0036379:9373-9392 Msa1455600:CDS 35.0%
TTTATCAAAATCGATGTGGC+AGG + tig0036379:9496-9515 Msa1455600:intron 35.0%
TAGACCTGAGTTAATTACTG+AGG + tig0036379:9553-9572 Msa1455600:CDS 35.0%
! ATAACTGTTTTGCATGCTTG+AGG - tig0036379:9703-9722 None:intergenic 35.0%
GCGACATATTATAACTCAAC+TGG + tig0036379:9776-9795 Msa1455600:CDS 35.0%
! GAGCATAATCTTCTCCATTT+TGG - tig0036379:9883-9902 None:intergenic 35.0%
GGAGAAGATTATGCTCAATT+TGG + tig0036379:9887-9906 Msa1455600:CDS 35.0%
ATAGACTCAATCAAGCCTTA+TGG + tig0036379:10101-10120 Msa1455600:intron 35.0%
CAAAACCTGCATTAAGAAGA+AGG - tig0036379:10260-10279 None:intergenic 35.0%
! ATCTAGAGTAAGCTTCTTCA+AGG + tig0036379:10282-10301 Msa1455600:CDS 35.0%
!! CTTTCAAGTTCATATGGTTC+CGG + tig0036379:10313-10332 Msa1455600:CDS 35.0%
!! CGGCGGATTTTCTATAATTT+TGG - tig0036379:10342-10361 None:intergenic 35.0%
AAATTATAGAAAATCCGCCG+AGG + tig0036379:10342-10361 Msa1455600:CDS 35.0%
!!! ACTATCAGTTCTGCTTTTTG+TGG - tig0036379:10404-10423 None:intergenic 35.0%
ATCAAGTCAGATAGCATTCA+AGG - tig0036379:10435-10454 None:intergenic 35.0%
!!! ATTTTCATAAAAAGTGCCCC+GGG - tig0036379:10488-10507 None:intergenic 35.0%
! AACTCCTTGATCTGTGATTT+TGG - tig0036379:10981-11000 None:intergenic 35.0%
ATAGGGATCAGAGATAGTAT+CGG + tig0036379:11236-11255 Msa1455600:intron 35.0%
ATTCCTGCTAATGCAAAAAC+TGG + tig0036379:11402-11421 Msa1455600:CDS 35.0%
TAATGCAAAAACTGGTTGCA+AGG + tig0036379:11410-11429 Msa1455600:CDS 35.0%
TCTAGGAACAATTCTATGCA+AGG + tig0036379:11528-11547 Msa1455600:CDS 35.0%
TATAACAGTGCTGTCATAGT+TGG + tig0036379:11564-11583 Msa1455600:CDS 35.0%
AAGTTTAGTCACCACATCTT+CGG + tig0036379:11596-11615 Msa1455600:CDS 35.0%
AGTTTAGGAGAAGGATTGAA+GGG - tig0036379:11622-11641 None:intergenic 35.0%
TAGTTTAGGAGAAGGATTGA+AGG - tig0036379:11623-11642 None:intergenic 35.0%
GGAGCTACATACTATAGTTT+AGG - tig0036379:11637-11656 None:intergenic 35.0%
AATATATTGAGTGTGAACCG+TGG + tig0036379:11769-11788 Msa1455600:three_prime_UTR 35.0%
GTAATCACGTTATTGATCTG+TGG + tig0036379:11940-11959 Msa1455600:three_prime_UTR 35.0%
!! GTGGATTTTGAGGGGTATAA+TGG - tig0036379:8010-8029 None:intergenic 40.0%
!! TTTTGTCGGTGGATTTTGAG+GGG - tig0036379:8018-8037 None:intergenic 40.0%
!! GTTTTGTCGGTGGATTTTGA+GGG - tig0036379:8019-8038 None:intergenic 40.0%
!! TGTTTTGTCGGTGGATTTTG+AGG - tig0036379:8020-8039 None:intergenic 40.0%
!!! AACGTGGTGTTGTGTTTTGT+CGG - tig0036379:8032-8051 None:intergenic 40.0%
TGAAGTTGGATATGAGAACG+TGG - tig0036379:8048-8067 None:intergenic 40.0%
GGAAATGTCCCTTATGAAGT+TGG - tig0036379:8062-8081 None:intergenic 40.0%
! ATGGTAATGAACGATATCGC+GGG - tig0036379:8140-8159 None:intergenic 40.0%
GTGTTGGAGGAAAATGGATA+TGG - tig0036379:8210-8229 None:intergenic 40.0%
AGTATACTCTGTCGTTGTGT+TGG - tig0036379:8226-8245 None:intergenic 40.0%
AACGACAGAGTATACTAGCT+AGG + tig0036379:8230-8249 Msa1455600:five_prime_UTR 40.0%
GAGTATACTAGCTAGGTTGT+TGG + tig0036379:8237-8256 Msa1455600:five_prime_UTR 40.0%
ATACTAGCTAGGTTGTTGGT+TGG + tig0036379:8241-8260 Msa1455600:five_prime_UTR 40.0%
TCAATGCAGTTTCAGATCGA+TGG - tig0036379:8303-8322 None:intergenic 40.0%
!! GAAAACAGAGTTTGGGGTTT+TGG - tig0036379:8492-8511 None:intergenic 40.0%
AGATCAAAAGGAGGATGTTG+TGG + tig0036379:8558-8577 Msa1455600:CDS 40.0%
CTTGCATTTCACAATGTGGT+TGG + tig0036379:8801-8820 Msa1455600:intron 40.0%
!! TGCCGATTCAATATCCAGTT+CGG - tig0036379:8903-8922 None:intergenic 40.0%
TATTGAATCGGCAAGATCTC+TGG + tig0036379:8910-8929 Msa1455600:intron 40.0%
!! ACTTTGGTGGGTTATTTAGG+CGG - tig0036379:8978-8997 None:intergenic 40.0%
CTACGGTTCAAATACTTTGG+TGG - tig0036379:8991-9010 None:intergenic 40.0%
CCGTAGCATGTGATTTGATT+TGG + tig0036379:9005-9024 Msa1455600:intron 40.0%
! TGCCCATTCTAGTTGGTTAA+CGG + tig0036379:9062-9081 Msa1455600:intron 40.0%
GACCGTTAACCAACTAGAAT+GGG - tig0036379:9067-9086 None:intergenic 40.0%
TGACCGTTAACCAACTAGAA+TGG - tig0036379:9068-9087 None:intergenic 40.0%
TGTTATTCATGTGATGCAGC+TGG + tig0036379:9092-9111 Msa1455600:intron 40.0%
! AATAAGTTGGAGAGGGGATT+CGG + tig0036379:9133-9152 Msa1455600:CDS 40.0%
CAAAAGGAATGTATGATGCC+GGG + tig0036379:9192-9211 Msa1455600:CDS 40.0%
AAAAGGAATGTATGATGCCG+GGG + tig0036379:9193-9212 Msa1455600:CDS 40.0%
!! TCGTGGGCAATTCTAGAATA+TGG + tig0036379:9263-9282 Msa1455600:CDS 40.0%
!! AATCTTGTGCTGGTTCCAAT+TGG - tig0036379:9310-9329 None:intergenic 40.0%
CGTTCCTCAGTAATTAACTC+AGG - tig0036379:9560-9579 None:intergenic 40.0%
GATACGTTCACTTGTAAGAG+GGG - tig0036379:9584-9603 None:intergenic 40.0%
GGATACGTTCACTTGTAAGA+GGG - tig0036379:9585-9604 None:intergenic 40.0%
AGGATACGTTCACTTGTAAG+AGG - tig0036379:9586-9605 None:intergenic 40.0%
! ATATGGGGATGAGCTTTTGT+GGG + tig0036379:9799-9818 Msa1455600:CDS 40.0%
AGTAAGCTTCTTCAAGGCAA+AGG + tig0036379:10288-10307 Msa1455600:CDS 40.0%
GTAAGCTTCTTCAAGGCAAA+GGG + tig0036379:10289-10308 Msa1455600:CDS 40.0%
!! AAGGGCCTTTCAAGTTCATA+TGG + tig0036379:10307-10326 Msa1455600:CDS 40.0%
CGGAACCATATGAACTTGAA+AGG - tig0036379:10315-10334 None:intergenic 40.0%
!!! TTTTCATAAAAAGTGCCCCG+GGG - tig0036379:10487-10506 None:intergenic 40.0%
AACGATCGTGACACTCTTTA+AGG - tig0036379:10579-10598 None:intergenic 40.0%
TCGAAGATCTGATGGTTTGA+AGG - tig0036379:10878-10897 None:intergenic 40.0%
ATGAATGCATACCTGAGATC+TGG - tig0036379:10908-10927 None:intergenic 40.0%
! TCCTCAGCATATGAGTTTTC+TGG + tig0036379:11329-11348 Msa1455600:CDS 40.0%
ACCAGAAAACTCATATGCTG+AGG - tig0036379:11333-11352 None:intergenic 40.0%
! TCAGCATATGAGTTTTCTGG+TGG + tig0036379:11332-11351 Msa1455600:CDS 40.0%
!! CAGCATATGAGTTTTCTGGT+GGG + tig0036379:11333-11352 Msa1455600:CDS 40.0%
!! ATGAGTTTTCTGGTGGGATA+TGG + tig0036379:11339-11358 Msa1455600:CDS 40.0%
CTCTGTGATGCACAAATTGT+GGG - tig0036379:11374-11393 None:intergenic 40.0%
! CAACCAGTTTTTGCATTAGC+AGG - tig0036379:11408-11427 None:intergenic 40.0%
CAAAAACTGGTTGCAAGGAT+GGG + tig0036379:11415-11434 Msa1455600:CDS 40.0%
ACAGCACTGTTATATGTGCT+TGG - tig0036379:11558-11577 None:intergenic 40.0%
!! CGACGCGGATTTAAGTTTTA+TGG + tig0036379:11684-11703 Msa1455600:three_prime_UTR 40.0%
CAATAGAATAAGCTCAGAGC+TGG - tig0036379:11860-11879 None:intergenic 40.0%
! TTGACTCATGCTAGCTTCGT+CGG - tig0036379:7966-7985 None:intergenic 45.0%
GTCGTTGTGTTGGAGGAAAA+TGG - tig0036379:8216-8235 None:intergenic 45.0%
ATACTCTGTCGTTGTGTTGG+AGG - tig0036379:8223-8242 None:intergenic 45.0%
CAAAAGGAGGATGTTGTGGT+TGG + tig0036379:8562-8581 Msa1455600:CDS 45.0%
!! GTTTGTGATTCTGGCTCTTG+TGG + tig0036379:8593-8612 Msa1455600:CDS 45.0%
! TTTTGATCAATGGCACCAGG+AGG - tig0036379:8693-8712 None:intergenic 45.0%
ATCGTGGTTTCATCGCAGTT+TGG - tig0036379:8951-8970 None:intergenic 45.0%
TTATTTAGGCGGCTCAATCG+TGG - tig0036379:8967-8986 None:intergenic 45.0%
ATGTTGCTGCCCATTCTAGT+TGG + tig0036379:9055-9074 Msa1455600:intron 45.0%
ACCAAACTTCATGTGATCCC+CGG - tig0036379:9213-9232 None:intergenic 45.0%
ACTGAGGCAGTAAAAGCCAT+AGG - tig0036379:9239-9258 None:intergenic 45.0%
GGAGATCAAATGGATGCTGT+TGG + tig0036379:9284-9303 Msa1455600:CDS 45.0%
AATGGATGCTGTTGGCCAAT+TGG + tig0036379:9292-9311 Msa1455600:CDS 45.0%
AACCAGCACAAGATTCTCTC+AGG + tig0036379:9315-9334 Msa1455600:CDS 45.0%
TCTCGTCAATGCTCATCCTT+CGG + tig0036379:9352-9371 Msa1455600:CDS 45.0%
TCAAAATCGATGTGGCAGGT+TGG + tig0036379:9500-9519 Msa1455600:intron 45.0%
CAACACTTGTGATCTGCAAC+AGG - tig0036379:9530-9549 None:intergenic 45.0%
CTGTTGCAGATCACAAGTGT+TGG + tig0036379:9528-9547 Msa1455600:CDS 45.0%
TTCAGCTGCAACATCTGATC+CGG - tig0036379:9612-9631 None:intergenic 45.0%
AGCAATGGCATCAGCATCTT+TGG + tig0036379:9640-9659 Msa1455600:CDS 45.0%
! ACTTTTGCGGACAAGCATAG+AGG + tig0036379:9725-9744 Msa1455600:CDS 45.0%
! GATATGGGGATGAGCTTTTG+TGG + tig0036379:9798-9817 Msa1455600:CDS 45.0%
AGGAATCATCACCTGTAGCA+TGG - tig0036379:9841-9860 None:intergenic 45.0%
TCCTACCTTCAGTATGTGAC+TGG + tig0036379:9857-9876 Msa1455600:CDS 45.0%
TTTGGCCAGTCACATACTGA+AGG - tig0036379:9865-9884 None:intergenic 45.0%
! CAGTATGTGACTGGCCAAAA+TGG + tig0036379:9866-9885 Msa1455600:CDS 45.0%
AGCTCTTCATACCTGAGTTC+CGG - tig0036379:9948-9967 None:intergenic 45.0%
AAGACCACACATTACAGCCT+CGG - tig0036379:10362-10381 None:intergenic 45.0%
!!! GTTCTGCTTTTTGTGGCTGT+TGG - tig0036379:10397-10416 None:intergenic 45.0%
!! TTTCATAAAAAGTGCCCCGG+GGG - tig0036379:10486-10505 None:intergenic 45.0%
GTCTGGAGGAACACAAACTA+TGG + tig0036379:10653-10672 Msa1455600:intron 45.0%
GTCTGATATGGGTGAGTGAA+TGG + tig0036379:10752-10771 Msa1455600:CDS 45.0%
GCTAAGAATGCAGATGCAAC+AGG - tig0036379:10793-10812 None:intergenic 45.0%
GCAAAGTCTCGAAGATCTGA+TGG - tig0036379:10886-10905 None:intergenic 45.0%
TTCCAGGCTGATTACGTGTT+GGG + tig0036379:11300-11319 Msa1455600:intron 45.0%
CCTCTGTGATGCACAAATTG+TGG - tig0036379:11375-11394 None:intergenic 45.0%
CCACAATTTGTGCATCACAG+AGG + tig0036379:11372-11391 Msa1455600:CDS 45.0%
GCAAAAACTGGTTGCAAGGA+TGG + tig0036379:11414-11433 Msa1455600:CDS 45.0%
AGCTACATTGGGATTCGGAT+CGG - tig0036379:11460-11479 None:intergenic 45.0%
CCAGTAGCTACATTGGGATT+CGG - tig0036379:11465-11484 None:intergenic 45.0%
AGTGCTCCAGTAGCTACATT+GGG - tig0036379:11471-11490 None:intergenic 45.0%
AAGTGCTCCAGTAGCTACAT+TGG - tig0036379:11472-11491 None:intergenic 45.0%
ACTCAAAAGAAGTTGCGACG+CGG + tig0036379:11669-11688 Msa1455600:three_prime_UTR 45.0%
ACTAACCTATCCAGACACCA+CGG - tig0036379:11789-11808 None:intergenic 45.0%
!!! GTGGTGTTGTGTTTTGTCGG+TGG - tig0036379:8029-8048 None:intergenic 50.0%
ATGGGGAGAAGGAGTCGAAA+TGG - tig0036379:8159-8178 None:intergenic 50.0%
!! GTGATTCTGGCTCTTGTGGT+CGG + tig0036379:8597-8616 Msa1455600:CDS 50.0%
TCGGCAAGATCTCTGGATCA+TGG + tig0036379:8917-8936 Msa1455600:intron 50.0%
TTACTGCCTCAGTGCTATCG+TGG + tig0036379:9246-9265 Msa1455600:CDS 50.0%
TACTGCCTCAGTGCTATCGT+GGG + tig0036379:9247-9266 Msa1455600:CDS 50.0%
AATTGCCCACGATAGCACTG+AGG - tig0036379:9255-9274 None:intergenic 50.0%
CACCTGAGAGAATCTTGTGC+TGG - tig0036379:9320-9339 None:intergenic 50.0%
CAGCACAAGATTCTCTCAGG+TGG + tig0036379:9318-9337 Msa1455600:CDS 50.0%
CAAGTGAACGTATCCTGTCC+CGG + tig0036379:9590-9609 Msa1455600:CDS 50.0%
TCAGCTGCAACATCTGATCC+GGG - tig0036379:9611-9630 None:intergenic 50.0%
AGCTGAAACTGCAGCAGCAA+TGG + tig0036379:9625-9644 Msa1455600:CDS 50.0%
GCCAGTCACATACTGAAGGT+AGG - tig0036379:9861-9880 None:intergenic 50.0%
CTCAATTTGGAAGCCCAACC+TGG + tig0036379:9900-9919 Msa1455600:CDS 50.0%
AAGCCCAACCTGGTTCAGTT+GGG + tig0036379:9910-9929 Msa1455600:CDS 50.0%
TGTCCCAACTGAACCAGGTT+GGG - tig0036379:9916-9935 None:intergenic 50.0%
TTGTCCCAACTGAACCAGGT+TGG - tig0036379:9917-9936 None:intergenic 50.0%
! CTTGTTGTCCCAACTGAACC+AGG - tig0036379:9921-9940 None:intergenic 50.0%
AGTTGGGACAACAAGCTTGC+CGG + tig0036379:9926-9945 Msa1455600:CDS 50.0%
TTTGTGGCTGTTGGAGAGTC+TGG - tig0036379:10388-10407 None:intergenic 50.0%
GAAATGCTAATGAGTGCCCC+CGG + tig0036379:10467-10486 Msa1455600:intron 50.0%
AAATGCTAATGAGTGCCCCC+GGG + tig0036379:10468-10487 Msa1455600:intron 50.0%
TCGAGACTTTGCCAGATCTC+AGG + tig0036379:10894-10913 Msa1455600:CDS 50.0%
CTTCCAGGCTGATTACGTGT+TGG + tig0036379:11299-11318 Msa1455600:intron 50.0%
AAACTGGTTGCAAGGATGGG+TGG + tig0036379:11418-11437 Msa1455600:CDS 50.0%
CCGAATCCCAATGTAGCTAC+TGG + tig0036379:11462-11481 Msa1455600:CDS 50.0%
! GTAGCTACTGGAGCACTTGT+TGG + tig0036379:11474-11493 Msa1455600:CDS 50.0%
! GCGTCGCAACTTCTTTTGAG+TGG - tig0036379:11670-11689 None:intergenic 50.0%
GAATAAGCTCAGAGCTGGTG+AGG - tig0036379:11855-11874 None:intergenic 50.0%
! CTCATGCTAGCTTCGTCGGT+GGG - tig0036379:7962-7981 None:intergenic 55.0%
! ACTCATGCTAGCTTCGTCGG+TGG - tig0036379:7963-7982 None:intergenic 55.0%
! GCTAGGTTGTTGGTTGGTGC+TGG + tig0036379:8247-8266 Msa1455600:five_prime_UTR 55.0%
! GATAAACCCGAACCGGTTCC+CGG + tig0036379:8666-8685 Msa1455600:CDS 55.0%
ATCAATGGCACCAGGAGGAC+CGG - tig0036379:8688-8707 None:intergenic 55.0%
GAGAGGGGATTCGGCTCTTA+AGG + tig0036379:9142-9161 Msa1455600:CDS 55.0%
GGGGATTCGGCTCTTAAGGA+TGG + tig0036379:9146-9165 Msa1455600:CDS 55.0%
GCCGGGGATCACATGAAGTT+TGG + tig0036379:9209-9228 Msa1455600:CDS 55.0%
TGCAACATCTGATCCGGGAC+AGG - tig0036379:9606-9625 None:intergenic 55.0%
! AGCTTTTGTGGGCAGCTACC+TGG + tig0036379:9810-9829 Msa1455600:CDS 55.0%
!! ACCTGGCTCTACCATGCTAC+AGG + tig0036379:9827-9846 Msa1455600:CDS 55.0%
ACCTGTAGCATGGTAGAGCC+AGG - tig0036379:9831-9850 None:intergenic 55.0%
GAAGCCCAACCTGGTTCAGT+TGG + tig0036379:9909-9928 Msa1455600:CDS 55.0%
CAACAAGCTTGCCGGAACTC+AGG + tig0036379:9934-9953 Msa1455600:CDS 55.0%
ACCACACATTACAGCCTCGG+CGG - tig0036379:10359-10378 None:intergenic 55.0%
AATGCTAATGAGTGCCCCCG+GGG + tig0036379:10469-10488 Msa1455600:intron 55.0%
TGCCCAACACGTAATCAGCC+TGG - tig0036379:11305-11324 None:intergenic 55.0%
! GCTACTGGAGCACTTGTTGG+TGG + tig0036379:11477-11496 Msa1455600:CDS 55.0%
GGATTGAAGGGCCGAAGATG+TGG - tig0036379:11610-11629 None:intergenic 55.0%
TGAGTGTGAACCGTGGTGTC+TGG + tig0036379:11776-11795 Msa1455600:three_prime_UTR 55.0%
GTGAACCGTGGTGTCTGGAT+AGG + tig0036379:11781-11800 Msa1455600:three_prime_UTR 55.0%
TGCGGCGAGAAATGGGGAGA+AGG - tig0036379:8170-8189 None:intergenic 60.0%
TCCCCATTTCTCGCCGCAAC+CGG + tig0036379:8172-8191 Msa1455600:five_prime_UTR 60.0%
ACCCGGTTGCGGCGAGAAAT+GGG - tig0036379:8177-8196 None:intergenic 60.0%
ATTTCTCGCCGCAACCGGGT+CGG + tig0036379:8177-8196 Msa1455600:five_prime_UTR 60.0%
GATATGGGTCTGACCCGACC+CGG - tig0036379:8194-8213 None:intergenic 60.0%
TCAATGGCACCAGGAGGACC+GGG - tig0036379:8687-8706 None:intergenic 60.0%
TCCGCCGAGGCTGTAATGTG+TGG + tig0036379:10355-10374 Msa1455600:CDS 60.0%
GTTGCAAGGATGGGTGGCAG+TGG + tig0036379:11424-11443 Msa1455600:CDS 60.0%
CCCGGTTGCGGCGAGAAATG+GGG - tig0036379:8176-8195 None:intergenic 65.0%
CCCCATTTCTCGCCGCAACC+GGG + tig0036379:8173-8192 Msa1455600:five_prime_UTR 65.0%
GACCCGGTTGCGGCGAGAAA+TGG - tig0036379:8178-8197 None:intergenic 65.0%
TTTCTCGCCGCAACCGGGTC+GGG + tig0036379:8178-8197 Msa1455600:five_prime_UTR 65.0%
AGGAGGACCGGGAACCGGTT+CGG - tig0036379:8676-8695 None:intergenic 65.0%
GGTCTGACCCGACCCGGTTG+CGG - tig0036379:8188-8207 None:intergenic 70.0%
! GGAGGACCGGGAACCGGTTC+GGG - tig0036379:8675-8694 None:intergenic 70.0%
! GAACCGGTTCCCGGTCCTCC+TGG + tig0036379:8675-8694 Msa1455600:CDS 70.0%
GCACCAGGAGGACCGGGAAC+CGG - tig0036379:8681-8700 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
tig0036379 gene 7955 11988 7955 ID=Msa1455600;Name=Msa1455600
tig0036379 mRNA 7955 11988 7955 ID=Msa1455600-mRNA-1;Parent=Msa1455600;Name=Msa1455600-mRNA-1;_AED=0.03;_eAED=0.03;_QI=588|1|1|1|1|1|6|350|529
tig0036379 exon 7955 8762 7955 ID=Msa1455600-mRNA-1:exon:20327;Parent=Msa1455600-mRNA-1
tig0036379 exon 9111 9394 9111 ID=Msa1455600-mRNA-1:exon:20328;Parent=Msa1455600-mRNA-1
tig0036379 exon 9518 9955 9518 ID=Msa1455600-mRNA-1:exon:20329;Parent=Msa1455600-mRNA-1
tig0036379 exon 10274 10421 10274 ID=Msa1455600-mRNA-1:exon:20330;Parent=Msa1455600-mRNA-1
tig0036379 exon 10749 10915 10749 ID=Msa1455600-mRNA-1:exon:20331;Parent=Msa1455600-mRNA-1
tig0036379 exon 11306 11988 11306 ID=Msa1455600-mRNA-1:exon:20332;Parent=Msa1455600-mRNA-1
tig0036379 five_prime_UTR 7955 8542 7955 ID=Msa1455600-mRNA-1:five_prime_utr;Parent=Msa1455600-mRNA-1
tig0036379 CDS 8543 8762 8543 ID=Msa1455600-mRNA-1:cds;Parent=Msa1455600-mRNA-1
tig0036379 CDS 9111 9394 9111 ID=Msa1455600-mRNA-1:cds;Parent=Msa1455600-mRNA-1
tig0036379 CDS 9518 9955 9518 ID=Msa1455600-mRNA-1:cds;Parent=Msa1455600-mRNA-1
tig0036379 CDS 10274 10421 10274 ID=Msa1455600-mRNA-1:cds;Parent=Msa1455600-mRNA-1
tig0036379 CDS 10749 10915 10749 ID=Msa1455600-mRNA-1:cds;Parent=Msa1455600-mRNA-1
tig0036379 CDS 11306 11638 11306 ID=Msa1455600-mRNA-1:cds;Parent=Msa1455600-mRNA-1
tig0036379 three_prime_UTR 11639 11988 11639 ID=Msa1455600-mRNA-1:three_prime_utr;Parent=Msa1455600-mRNA-1
Gene Sequence

>Msa1455600

ATGAGAGAAAAATCGAGATCAAAAGGAGGATGTTGTGGTTGGTTTATTGCGTTTGTGATTCTGGCTCTTGTGGTCGGTGCTATTGTTTATGCTGTTAAGAAGAAAATCGATAACTCAAATTCAGATAAACCCGAACCGGTTCCCGGTCCTCCTGGTGCCATTGATCAAAAATATGCTTCTGCTCTCAAAACTGCAATGCAATTCTTCGACGTGCAAAAATCTGGCAAGTTAGAGAACAACAAAATAAGTTGGAGAGGGGATTCGGCTCTTAAGGATGGAAAGCAAGCAGATTTAGATCTAACAAAAGGAATGTATGATGCCGGGGATCACATGAAGTTTGGTTTTCCTATGGCTTTTACTGCCTCAGTGCTATCGTGGGCAATTCTAGAATATGGAGATCAAATGGATGCTGTTGGCCAATTGGAACCAGCACAAGATTCTCTCAGGTGGATCACTGATTTTCTCGTCAATGCTCATCCTTCGGAGAATGTTCTGTATATTCAGGTTGGTGATCCTGTTGCAGATCACAAGTGTTGGAATAGACCTGAGTTAATTACTGAGGAACGACCCCTCTTACAAGTGAACGTATCCTGTCCCGGATCAGATGTTGCAGCTGAAACTGCAGCAGCAATGGCATCAGCATCTTTGGTCTTCAAAAAGTCAGATGCTACATATTCAAGCACTCTCCTCAAGCATGCAAAACAGTTATTTACTTTTGCGGACAAGCATAGAGGAATCTACAGTGAGAATATACCTGAAGTTGCGACATATTATAACTCAACTGGATATGGGGATGAGCTTTTGTGGGCAGCTACCTGGCTCTACCATGCTACAGGTGATGATTCCTACCTTCAGTATGTGACTGGCCAAAATGGAGAAGATTATGCTCAATTTGGAAGCCCAACCTGGTTCAGTTGGGACAACAAGCTTGCCGGAACTCAGGTTTTGTTATCTAGAGTAAGCTTCTTCAAGGCAAAGGGCCTTTCAAGTTCATATGGTTCCGGCCTCCAAAATTATAGAAAATCCGCCGAGGCTGTAATGTGTGGTCTTCTTCCAGACTCTCCAACAGCCACAAAAAGCAGAACTGATAATGGTCTGATATGGGTGAGTGAATGGAACGCTTTGCAGCATCCTGTTGCATCTGCATTCTTAGCTTCTGTTTACAGTGACTATATGCTTACAACGCAGACTCCCAATATAAAATGTGATTCAGATTCCTTCAAACCATCAGATCTTCGAGACTTTGCCAGATCTCAGGCTGATTACGTGTTGGGCAAGAATCCTCAGCATATGAGTTTTCTGGTGGGATATGGAAAGAATTTCCCACAATTTGTGCATCACAGAGGAGCTTCAATTCCTGCTAATGCAAAAACTGGTTGCAAGGATGGGTGGCAGTGGCTTGACTCATCCGATCCGAATCCCAATGTAGCTACTGGAGCACTTGTTGGTGGACCTTTCTTAAATGAGACTTACATTGATTCTAGGAACAATTCTATGCAAGGAGAACCAAGCACATATAACAGTGCTGTCATAGTTGGTCTCTTATCAAGTTTAGTCACCACATCTTCGGCCCTTCAATCCTTCTCCTAA
Protein sequence

>Msa1455600

MREKSRSKGGCCGWFIAFVILALVVGAIVYAVKKKIDNSNSDKPEPVPGPPGAIDQKYASALKTAMQFFDVQKSGKLENNKISWRGDSALKDGKQADLDLTKGMYDAGDHMKFGFPMAFTASVLSWAILEYGDQMDAVGQLEPAQDSLRWITDFLVNAHPSENVLYIQVGDPVADHKCWNRPELITEERPLLQVNVSCPGSDVAAETAAAMASASLVFKKSDATYSSTLLKHAKQLFTFADKHRGIYSENIPEVATYYNSTGYGDELLWAATWLYHATGDDSYLQYVTGQNGEDYAQFGSPTWFSWDNKLAGTQVLLSRVSFFKAKGLSSSYGSGLQNYRKSAEAVMCGLLPDSPTATKSRTDNGLIWVSEWNALQHPVASAFLASVYSDYMLTTQTPNIKCDSDSFKPSDLRDFARSQADYVLGKNPQHMSFLVGYGKNFPQFVHHRGASIPANAKTGCKDGWQWLDSSDPNPNVATGALVGGPFLNETYIDSRNNSMQGEPSTYNSAVIVGLLSSLVTTSSALQSFS*