AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1465250

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1465250 MtrunA17_Chr7g0265301 97.541 122 3 0 8 129 99 220 3.74e-82 240
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1465250 AT3G53120.1 71.311 122 35 0 8 129 96 217 4.96e-58 179
Msa1465250 AT2G36680.1 65.574 122 42 0 8 129 96 217 8.24e-54 168

Find 64 sgRNAs with CRISPR-Local

Find 214 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCGTACCTCTTCTTAATA+TGG 0.239168 tig0045092:+392 Msa1465250:intergenic
AAGTCATACCTTGAATTCTT+TGG 0.285394 tig0045092:+851 Msa1465250:intergenic
GAGACATGTGAAAAGGAAAT+TGG 0.287879 tig0045092:+1815 Msa1465250:intergenic
CTCATTAGCAGTAGCTAATT+CGG 0.305961 tig0045092:+939 Msa1465250:intergenic
ATGAACTTGCCAAGGAAAAT+AGG 0.312092 tig0045092:-1397 Msa1465250:CDS
CAGTATGGCAACTATTTGTT+AGG 0.319844 tig0045092:+1688 Msa1465250:intergenic
AATAGTCCGCAGACATGTAT+TGG 0.360547 tig0045092:+1635 Msa1465250:intergenic
CATGGATAATGATACATAAA+AGG 0.366360 tig0045092:+1764 Msa1465250:intergenic
ATGGCGCATAACTACTCAAC+AGG 0.375445 tig0045092:+411 Msa1465250:intergenic
TCTCTCTGTCAAGGAGTTGC+TGG 0.377858 tig0045092:+689 Msa1465250:intergenic
CATGGATGATGATACATAAA+AGG 0.380010 tig0045092:+1734 Msa1465250:intergenic
TATCATCATCCATGTGACTA+TGG 0.382443 tig0045092:-1725 Msa1465250:five_prime_UTR
TTTCAGATTCCTCATCTGTC+TGG 0.388757 tig0045092:+719 Msa1465250:intergenic
ATCCACACAACTCATAAAAC+TGG 0.410678 tig0045092:+531 Msa1465250:intergenic
GTGCCTCAAATGCCTAGAAC+AGG 0.413227 tig0045092:+1853 Msa1465250:intergenic
ATTATATGAGCCTTGACATA+TGG 0.419725 tig0045092:-577 Msa1465250:exon
CATGATGGAACTTAGGAATC+AGG 0.425059 tig0045092:-1244 Msa1465250:intron
TCCCTTCTCCAAAGAATTCA+AGG 0.425395 tig0045092:-859 Msa1465250:intron
TTCTAGGCATTTGAGGCACC+TGG 0.440576 tig0045092:-1849 Msa1465250:five_prime_UTR
TTATGAATACTAGTCGCTAC+AGG 0.447462 tig0045092:+448 Msa1465250:intergenic
TGTTGTCATTGTTGCAGTGA+TGG 0.460019 tig0045092:-1993 Msa1465250:five_prime_UTR
TACCTTGAATTCTTTGGAGA+AGG 0.465604 tig0045092:+857 Msa1465250:intergenic
TATCACTGAATCTGTGTCAA+TGG 0.468438 tig0045092:-1919 Msa1465250:five_prime_UTR
GAATGTTGATGTGTGTTGAT+AGG 0.474173 tig0045092:-1949 Msa1465250:five_prime_UTR
TGTGACTATGGTAACAAACT+TGG 0.476171 tig0045092:-1713 Msa1465250:five_prime_UTR
ATTCTTTGGAGAAGGGATGC+AGG 0.482592 tig0045092:+865 Msa1465250:intergenic
ATAGTCCGCAGACATGTATT+GGG 0.486779 tig0045092:+1636 Msa1465250:intergenic
TACAGAGGAAAATCTACAAA+AGG 0.489564 tig0045092:-1274 Msa1465250:intron
GTCGGTTGAGACATGTGAAA+AGG 0.496541 tig0045092:+1808 Msa1465250:intergenic
GAGAGATGAAGCGTTAGTTA+AGG 0.502670 tig0045092:+1786 Msa1465250:intergenic
GTGTCAATGGAATATAAGAT+TGG 0.503867 tig0045092:-1906 Msa1465250:five_prime_UTR
CGCCATATTAAGAAGAGGTA+CGG 0.512104 tig0045092:-394 Msa1465250:three_prime_UTR
ACCTTGAATTCTTTGGAGAA+GGG 0.513047 tig0045092:+858 Msa1465250:intergenic
TTCTTTGGAGAAGGGATGCA+GGG 0.513967 tig0045092:+866 Msa1465250:intergenic
AAAACTTCAGCACACTCGTA+CGG 0.523060 tig0045092:+1543 Msa1465250:intergenic
AAACTTCAGCACACTCGTAC+GGG 0.525043 tig0045092:+1544 Msa1465250:intergenic
TGCCAAGGAAAATAGGCAGC+TGG 0.538132 tig0045092:-1390 Msa1465250:CDS
ACAAAAGGAACCTCGCATGA+TGG 0.541626 tig0045092:-1259 Msa1465250:CDS
AAGAGTTCTCTTGTGGTAAG+TGG 0.544139 tig0045092:+624 Msa1465250:intergenic
ATGTGAAAAGGAAATTGGAA+TGG 0.553977 tig0045092:+1820 Msa1465250:intergenic
TGAAAAGACTATTGAAATGA+CGG 0.562977 tig0045092:-1667 Msa1465250:five_prime_UTR
AAGGAAAATAGGCAGCTGGC+AGG 0.567548 tig0045092:-1386 Msa1465250:intron
GCCTCAAATGCCTAGAACAG+GGG 0.584844 tig0045092:+1855 Msa1465250:intergenic
CAAGGTCAATCTCTCTGTCA+AGG 0.586704 tig0045092:+680 Msa1465250:intergenic
AACCTCGCATGATGGAACTT+AGG 0.589339 tig0045092:-1251 Msa1465250:CDS
ACTGTGCTCTACTGTACAGT+TGG 0.589520 tig0045092:-1568 Msa1465250:five_prime_UTR
GCTGAAAGATGAACTTGCCA+AGG 0.593928 tig0045092:-1405 Msa1465250:CDS
TGCCTCAAATGCCTAGAACA+GGG 0.595745 tig0045092:+1854 Msa1465250:intergenic
TTTCAGCTGATCATATCCAT+AGG 0.602180 tig0045092:+1421 Msa1465250:intergenic
GATGAAGCGTTAGTTAAGGT+CGG 0.603371 tig0045092:+1790 Msa1465250:intergenic
GGAATATAAGATTGGACATG+CGG 0.604981 tig0045092:-1898 Msa1465250:five_prime_UTR
GTGACTATGGTAACAAACTT+GGG 0.608763 tig0045092:-1712 Msa1465250:five_prime_UTR
TACATAAAAGGAGAGTCGCA+TGG 0.623514 tig0045092:+1746 Msa1465250:intergenic
ATCTGTGAACCAGACAGATG+AGG 0.626864 tig0045092:-728 Msa1465250:CDS
TATGAATACTAGTCGCTACA+GGG 0.629918 tig0045092:+449 Msa1465250:intergenic
TTATGCGCCATATTAAGAAG+AGG 0.632520 tig0045092:-399 Msa1465250:three_prime_UTR
AAATTGGAATGGAAAGAACC+AGG 0.637162 tig0045092:+1831 Msa1465250:intergenic
TTCCTAAGTTCCATCATGCG+AGG 0.642796 tig0045092:+1249 Msa1465250:intergenic
CCTCAAATGCCTAGAACAGG+GGG 0.650247 tig0045092:+1856 Msa1465250:intergenic
TCTTTGGAGAAGGGATGCAG+GGG 0.657685 tig0045092:+867 Msa1465250:intergenic
GACATCCCAATACATGTCTG+CGG 0.678510 tig0045092:-1641 Msa1465250:five_prime_UTR
AGTTTGTTACCATAGTCACA+TGG 0.679017 tig0045092:+1716 Msa1465240:intergenic
GATGTACAAGAGTTCTCTTG+TGG 0.682514 tig0045092:+617 Msa1465240:intergenic
TGGCGCATAACTACTCAACA+GGG 0.707942 tig0045092:+412 Msa1465240:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAAACAAAACTAAAATA+TGG + tig0045092:1934-1953 Msa1465250:intergenic 10.0%
!! TTAAAAACAAAACTAAAATA+TGG + tig0045092:1934-1953 Msa1465250:intergenic 10.0%
!!! TTTTAATTTACTACATTCAA+CGG - tig0045092:1948-1967 Msa1465250:five_prime_UTR 15.0%
!!! TTTTAATTTACTACATTCAA+CGG - tig0045092:1948-1967 Msa1465250:five_prime_UTR 15.0%
!!! CTTGTGTTTTAACTATTTTA+TGG - tig0045092:847-866 Msa1465250:intron 20.0%
!! CATAATCTATTAATCAAGAT+TGG - tig0045092:1198-1217 Msa1465250:intron 20.0%
!!! CTTGTGTTTTAACTATTTTA+TGG - tig0045092:847-866 Msa1465250:intron 20.0%
!! CATAATCTATTAATCAAGAT+TGG - tig0045092:1198-1217 Msa1465250:intron 20.0%
! CATGGATAATGATACATAAA+AGG + tig0045092:530-549 Msa1465250:intergenic 25.0%
!! TTTCAATAGTCTTTTCAGTA+TGG + tig0045092:621-640 Msa1465250:intergenic 25.0%
! TGAAAAGACTATTGAAATGA+CGG - tig0045092:624-643 Msa1465250:CDS 25.0%
! AACAAAAACACTGCTAAAAA+AGG + tig0045092:799-818 Msa1465250:intergenic 25.0%
!!! TGTTTTTGTTTTTGTTCTGA+TGG - tig0045092:809-828 Msa1465250:intron 25.0%
!! TTTAACTATTTTATGGCCTA+TGG - tig0045092:854-873 Msa1465250:intron 25.0%
! AGTTCTATAACACTTATTGA+TGG + tig0045092:979-998 Msa1465250:intergenic 25.0%
! CAAATTTGTCTTTAATACAG+AGG - tig0045092:1002-1021 Msa1465250:intron 25.0%
!! TTTTCCAAAAATTCCAATCA+GGG + tig0045092:1104-1123 Msa1465250:intergenic 25.0%
!! ATTTTCCAAAAATTCCAATC+AGG + tig0045092:1105-1124 Msa1465250:intergenic 25.0%
! ATCTATTAATCAAGATTGGA+CGG - tig0045092:1202-1221 Msa1465250:intron 25.0%
! ACAATTTATTGCAATAGTTC+TGG - tig0045092:1471-1490 Msa1465250:five_prime_UTR 25.0%
!! TTTTCATATTCCATATGTCA+AGG + tig0045092:1727-1746 Msa1465250:intergenic 25.0%
! CATGGATAATGATACATAAA+AGG + tig0045092:530-549 Msa1465250:intergenic 25.0%
!! TTTCAATAGTCTTTTCAGTA+TGG + tig0045092:621-640 Msa1465250:intergenic 25.0%
! TGAAAAGACTATTGAAATGA+CGG - tig0045092:624-643 Msa1465250:CDS 25.0%
! AACAAAAACACTGCTAAAAA+AGG + tig0045092:799-818 Msa1465250:intergenic 25.0%
!!! TGTTTTTGTTTTTGTTCTGA+TGG - tig0045092:809-828 Msa1465250:intron 25.0%
!! TTTAACTATTTTATGGCCTA+TGG - tig0045092:854-873 Msa1465250:intron 25.0%
! AGTTCTATAACACTTATTGA+TGG + tig0045092:979-998 Msa1465250:intergenic 25.0%
! CAAATTTGTCTTTAATACAG+AGG - tig0045092:1002-1021 Msa1465250:intron 25.0%
!! TTTTCCAAAAATTCCAATCA+GGG + tig0045092:1104-1123 Msa1465250:intergenic 25.0%
!! ATTTTCCAAAAATTCCAATC+AGG + tig0045092:1105-1124 Msa1465250:intergenic 25.0%
! ATCTATTAATCAAGATTGGA+CGG - tig0045092:1202-1221 Msa1465250:intron 25.0%
! ACAATTTATTGCAATAGTTC+TGG - tig0045092:1471-1490 Msa1465250:five_prime_UTR 25.0%
!! TTTTCATATTCCATATGTCA+AGG + tig0045092:1727-1746 Msa1465250:intergenic 25.0%
GTGTCAATGGAATATAAGAT+TGG - tig0045092:385-404 Msa1465250:three_prime_UTR 30.0%
ATGTGAAAAGGAAATTGGAA+TGG + tig0045092:474-493 Msa1465250:intergenic 30.0%
CATGGATGATGATACATAAA+AGG + tig0045092:560-579 Msa1465250:intergenic 30.0%
TGCTAAAAAAGGTTAAGCAT+AGG + tig0045092:788-807 Msa1465250:intergenic 30.0%
!!! TTTTGTTTTTGTTCTGATGG+TGG - tig0045092:812-831 Msa1465250:intron 30.0%
TACAGAGGAAAATCTACAAA+AGG - tig0045092:1017-1036 Msa1465250:intron 30.0%
TTTCCAAAAATTCCAATCAG+GGG + tig0045092:1103-1122 Msa1465250:intergenic 30.0%
! AAATGAGTTTTCTGCAGTTA+AGG - tig0045092:1121-1140 Msa1465250:intron 30.0%
CCAAAATCTTATGCAGATTA+CGG - tig0045092:1281-1300 Msa1465250:intron 30.0%
TTATTCTACACTGTTTCACA+AGG + tig0045092:1323-1342 Msa1465250:intergenic 30.0%
AAGTCATACCTTGAATTCTT+TGG + tig0045092:1443-1462 Msa1465250:intergenic 30.0%
!! ATTTATTGCAATAGTTCTGG+TGG - tig0045092:1474-1493 Msa1465250:five_prime_UTR 30.0%
!!! TTTTTTCACTATTACGACGA+AGG - tig0045092:1503-1522 Msa1465250:five_prime_UTR 30.0%
ATTATATGAGCCTTGACATA+TGG - tig0045092:1714-1733 Msa1465250:five_prime_UTR 30.0%
! ATTCAACGGTATTTTTCTGA+AGG - tig0045092:1962-1981 Msa1465250:five_prime_UTR 30.0%
GTGTCAATGGAATATAAGAT+TGG - tig0045092:385-404 Msa1465250:three_prime_UTR 30.0%
ATGTGAAAAGGAAATTGGAA+TGG + tig0045092:474-493 Msa1465250:intergenic 30.0%
CATGGATGATGATACATAAA+AGG + tig0045092:560-579 Msa1465250:intergenic 30.0%
TGCTAAAAAAGGTTAAGCAT+AGG + tig0045092:788-807 Msa1465250:intergenic 30.0%
!!! TTTTGTTTTTGTTCTGATGG+TGG - tig0045092:812-831 Msa1465250:intron 30.0%
TACAGAGGAAAATCTACAAA+AGG - tig0045092:1017-1036 Msa1465250:intron 30.0%
TTTCCAAAAATTCCAATCAG+GGG + tig0045092:1103-1122 Msa1465250:intergenic 30.0%
! AAATGAGTTTTCTGCAGTTA+AGG - tig0045092:1121-1140 Msa1465250:intron 30.0%
CCAAAATCTTATGCAGATTA+CGG - tig0045092:1281-1300 Msa1465250:intron 30.0%
TTATTCTACACTGTTTCACA+AGG + tig0045092:1323-1342 Msa1465250:intergenic 30.0%
AAGTCATACCTTGAATTCTT+TGG + tig0045092:1443-1462 Msa1465250:intergenic 30.0%
!! ATTTATTGCAATAGTTCTGG+TGG - tig0045092:1474-1493 Msa1465250:five_prime_UTR 30.0%
!!! TTTTTTCACTATTACGACGA+AGG - tig0045092:1503-1522 Msa1465250:five_prime_UTR 30.0%
ATTATATGAGCCTTGACATA+TGG - tig0045092:1714-1733 Msa1465250:five_prime_UTR 30.0%
! ATTCAACGGTATTTTTCTGA+AGG - tig0045092:1962-1981 Msa1465250:five_prime_UTR 30.0%
! GAATGTTGATGTGTGTTGAT+AGG - tig0045092:342-361 Msa1465250:three_prime_UTR 35.0%
TATCACTGAATCTGTGTCAA+TGG - tig0045092:372-391 Msa1465250:three_prime_UTR 35.0%
GGAATATAAGATTGGACATG+CGG - tig0045092:393-412 Msa1465250:three_prime_UTR 35.0%
AAATTGGAATGGAAAGAACC+AGG + tig0045092:463-482 Msa1465250:intergenic 35.0%
GAGACATGTGAAAAGGAAAT+TGG + tig0045092:479-498 Msa1465250:intergenic 35.0%
TATCATCATCCATGTGACTA+TGG - tig0045092:566-585 Msa1465250:three_prime_UTR 35.0%
!! AGTTTGTTACCATAGTCACA+TGG + tig0045092:578-597 Msa1465250:intergenic 35.0%
TGTGACTATGGTAACAAACT+TGG - tig0045092:578-597 Msa1465250:exon 35.0%
GTGACTATGGTAACAAACTT+GGG - tig0045092:579-598 Msa1465250:exon 35.0%
CAGTATGGCAACTATTTGTT+AGG + tig0045092:606-625 Msa1465250:intergenic 35.0%
TTTCAGCTGATCATATCCAT+AGG + tig0045092:873-892 Msa1465250:intergenic 35.0%
ATGAACTTGCCAAGGAAAAT+AGG - tig0045092:894-913 Msa1465250:CDS 35.0%
TTAGGAATCAGGTAAGATCA+CGG - tig0045092:1058-1077 Msa1465250:intron 35.0%
AGGGGAAAAAACTAATCACA+GGG + tig0045092:1085-1104 Msa1465250:intergenic 35.0%
!!! GATTAGTTTTTTCCCCTGAT+TGG - tig0045092:1088-1107 Msa1465250:intron 35.0%
! TTTCCCCTGATTGGAATTTT+TGG - tig0045092:1097-1116 Msa1465250:intron 35.0%
! CCGTAATCTGCATAAGATTT+TGG + tig0045092:1284-1303 Msa1465250:intergenic 35.0%
CTCATTAGCAGTAGCTAATT+CGG + tig0045092:1355-1374 Msa1465250:intergenic 35.0%
! ACCTTGAATTCTTTGGAGAA+GGG + tig0045092:1436-1455 Msa1465250:intergenic 35.0%
! TACCTTGAATTCTTTGGAGA+AGG + tig0045092:1437-1456 Msa1465250:intergenic 35.0%
ATTTCTGCAAAAAAGCTGCA+AGG + tig0045092:1632-1651 Msa1465250:intergenic 35.0%
!! AACCAGTTTTATGAGTTGTG+TGG - tig0045092:1758-1777 Msa1465250:five_prime_UTR 35.0%
ATCCACACAACTCATAAAAC+TGG + tig0045092:1763-1782 Msa1465250:intergenic 35.0%
TATGAATACTAGTCGCTACA+GGG + tig0045092:1845-1864 Msa1465250:intergenic 35.0%
TTATGAATACTAGTCGCTAC+AGG + tig0045092:1846-1865 Msa1465250:intergenic 35.0%
TTATGCGCCATATTAAGAAG+AGG - tig0045092:1892-1911 Msa1465250:five_prime_UTR 35.0%
TTCCGTACCTCTTCTTAATA+TGG + tig0045092:1902-1921 Msa1465250:intergenic 35.0%
! GAATGTTGATGTGTGTTGAT+AGG - tig0045092:342-361 Msa1465250:three_prime_UTR 35.0%
TATCACTGAATCTGTGTCAA+TGG - tig0045092:372-391 Msa1465250:three_prime_UTR 35.0%
GGAATATAAGATTGGACATG+CGG - tig0045092:393-412 Msa1465250:three_prime_UTR 35.0%
AAATTGGAATGGAAAGAACC+AGG + tig0045092:463-482 Msa1465250:intergenic 35.0%
GAGACATGTGAAAAGGAAAT+TGG + tig0045092:479-498 Msa1465250:intergenic 35.0%
TATCATCATCCATGTGACTA+TGG - tig0045092:566-585 Msa1465250:three_prime_UTR 35.0%
!! AGTTTGTTACCATAGTCACA+TGG + tig0045092:578-597 Msa1465250:intergenic 35.0%
TGTGACTATGGTAACAAACT+TGG - tig0045092:578-597 Msa1465250:exon 35.0%
GTGACTATGGTAACAAACTT+GGG - tig0045092:579-598 Msa1465250:exon 35.0%
CAGTATGGCAACTATTTGTT+AGG + tig0045092:606-625 Msa1465250:intergenic 35.0%
TTTCAGCTGATCATATCCAT+AGG + tig0045092:873-892 Msa1465250:intergenic 35.0%
ATGAACTTGCCAAGGAAAAT+AGG - tig0045092:894-913 Msa1465250:CDS 35.0%
TTAGGAATCAGGTAAGATCA+CGG - tig0045092:1058-1077 Msa1465250:intron 35.0%
AGGGGAAAAAACTAATCACA+GGG + tig0045092:1085-1104 Msa1465250:intergenic 35.0%
!!! GATTAGTTTTTTCCCCTGAT+TGG - tig0045092:1088-1107 Msa1465250:intron 35.0%
! TTTCCCCTGATTGGAATTTT+TGG - tig0045092:1097-1116 Msa1465250:intron 35.0%
! CCGTAATCTGCATAAGATTT+TGG + tig0045092:1284-1303 Msa1465250:intergenic 35.0%
CTCATTAGCAGTAGCTAATT+CGG + tig0045092:1355-1374 Msa1465250:intergenic 35.0%
! ACCTTGAATTCTTTGGAGAA+GGG + tig0045092:1436-1455 Msa1465250:intergenic 35.0%
! TACCTTGAATTCTTTGGAGA+AGG + tig0045092:1437-1456 Msa1465250:intergenic 35.0%
ATTTCTGCAAAAAAGCTGCA+AGG + tig0045092:1632-1651 Msa1465250:intergenic 35.0%
!! AACCAGTTTTATGAGTTGTG+TGG - tig0045092:1758-1777 Msa1465250:five_prime_UTR 35.0%
ATCCACACAACTCATAAAAC+TGG + tig0045092:1763-1782 Msa1465250:intergenic 35.0%
TATGAATACTAGTCGCTACA+GGG + tig0045092:1845-1864 Msa1465250:intergenic 35.0%
TTATGAATACTAGTCGCTAC+AGG + tig0045092:1846-1865 Msa1465250:intergenic 35.0%
TTATGCGCCATATTAAGAAG+AGG - tig0045092:1892-1911 Msa1465250:five_prime_UTR 35.0%
TTCCGTACCTCTTCTTAATA+TGG + tig0045092:1902-1921 Msa1465250:intergenic 35.0%
TGTTGTCATTGTTGCAGTGA+TGG - tig0045092:298-317 Msa1465250:three_prime_UTR 40.0%
! GATGAAGCGTTAGTTAAGGT+CGG + tig0045092:504-523 Msa1465250:intergenic 40.0%
! GAGAGATGAAGCGTTAGTTA+AGG + tig0045092:508-527 Msa1465250:intergenic 40.0%
TACATAAAAGGAGAGTCGCA+TGG + tig0045092:548-567 Msa1465250:intergenic 40.0%
ATAGTCCGCAGACATGTATT+GGG + tig0045092:658-677 Msa1465250:intergenic 40.0%
AATAGTCCGCAGACATGTAT+TGG + tig0045092:659-678 Msa1465250:intergenic 40.0%
AAAACTTCAGCACACTCGTA+CGG + tig0045092:751-770 Msa1465250:intergenic 40.0%
! CATGATGGAACTTAGGAATC+AGG - tig0045092:1047-1066 Msa1465250:intron 40.0%
GGGGAAAAAACTAATCACAG+GGG + tig0045092:1084-1103 Msa1465250:intergenic 40.0%
CAGGGGAAAAAACTAATCAC+AGG + tig0045092:1086-1105 Msa1465250:intergenic 40.0%
TTCTACACTGTTTCACAAGG+AGG + tig0045092:1320-1339 Msa1465250:intergenic 40.0%
TCCCTTCTCCAAAGAATTCA+AGG - tig0045092:1432-1451 Msa1465250:exon 40.0%
TTTCAGATTCCTCATCTGTC+TGG + tig0045092:1575-1594 Msa1465250:intergenic 40.0%
AAGAGTTCTCTTGTGGTAAG+TGG + tig0045092:1670-1689 Msa1465250:intergenic 40.0%
GATGTACAAGAGTTCTCTTG+TGG + tig0045092:1677-1696 Msa1465250:intergenic 40.0%
CGCCATATTAAGAAGAGGTA+CGG - tig0045092:1897-1916 Msa1465250:five_prime_UTR 40.0%
TGTTGTCATTGTTGCAGTGA+TGG - tig0045092:298-317 Msa1465250:three_prime_UTR 40.0%
! GATGAAGCGTTAGTTAAGGT+CGG + tig0045092:504-523 Msa1465250:intergenic 40.0%
! GAGAGATGAAGCGTTAGTTA+AGG + tig0045092:508-527 Msa1465250:intergenic 40.0%
TACATAAAAGGAGAGTCGCA+TGG + tig0045092:548-567 Msa1465250:intergenic 40.0%
ATAGTCCGCAGACATGTATT+GGG + tig0045092:658-677 Msa1465250:intergenic 40.0%
AATAGTCCGCAGACATGTAT+TGG + tig0045092:659-678 Msa1465250:intergenic 40.0%
AAAACTTCAGCACACTCGTA+CGG + tig0045092:751-770 Msa1465250:intergenic 40.0%
! CATGATGGAACTTAGGAATC+AGG - tig0045092:1047-1066 Msa1465250:intron 40.0%
GGGGAAAAAACTAATCACAG+GGG + tig0045092:1084-1103 Msa1465250:intergenic 40.0%
CAGGGGAAAAAACTAATCAC+AGG + tig0045092:1086-1105 Msa1465250:intergenic 40.0%
TTCTACACTGTTTCACAAGG+AGG + tig0045092:1320-1339 Msa1465250:intergenic 40.0%
TCCCTTCTCCAAAGAATTCA+AGG - tig0045092:1432-1451 Msa1465250:exon 40.0%
TTTCAGATTCCTCATCTGTC+TGG + tig0045092:1575-1594 Msa1465250:intergenic 40.0%
AAGAGTTCTCTTGTGGTAAG+TGG + tig0045092:1670-1689 Msa1465250:intergenic 40.0%
GATGTACAAGAGTTCTCTTG+TGG + tig0045092:1677-1696 Msa1465250:intergenic 40.0%
CGCCATATTAAGAAGAGGTA+CGG - tig0045092:1897-1916 Msa1465250:five_prime_UTR 40.0%
TGCAATAAACCCCCTGTTCT+AGG - tig0045092:426-445 Msa1465250:three_prime_UTR 45.0%
TGCCTCAAATGCCTAGAACA+GGG + tig0045092:440-459 Msa1465250:intergenic 45.0%
GTCGGTTGAGACATGTGAAA+AGG + tig0045092:486-505 Msa1465250:intergenic 45.0%
GACATCCCAATACATGTCTG+CGG - tig0045092:650-669 Msa1465250:CDS 45.0%
ACTGTGCTCTACTGTACAGT+TGG - tig0045092:723-742 Msa1465250:CDS 45.0%
AAACTTCAGCACACTCGTAC+GGG + tig0045092:750-769 Msa1465250:intergenic 45.0%
GCTGAAAGATGAACTTGCCA+AGG - tig0045092:886-905 Msa1465250:CDS 45.0%
ACAAAAGGAACCTCGCATGA+TGG - tig0045092:1032-1051 Msa1465250:intron 45.0%
! AACCTCGCATGATGGAACTT+AGG - tig0045092:1040-1059 Msa1465250:intron 45.0%
TTCCTAAGTTCCATCATGCG+AGG + tig0045092:1045-1064 Msa1465250:intergenic 45.0%
! TTCTTTGGAGAAGGGATGCA+GGG + tig0045092:1428-1447 Msa1465250:intergenic 45.0%
! ATTCTTTGGAGAAGGGATGC+AGG + tig0045092:1429-1448 Msa1465250:intergenic 45.0%
TCACTATTACGACGAAGGCT+AGG - tig0045092:1508-1527 Msa1465250:five_prime_UTR 45.0%
ATCTGTGAACCAGACAGATG+AGG - tig0045092:1563-1582 Msa1465250:five_prime_UTR 45.0%
CAAGGTCAATCTCTCTGTCA+AGG + tig0045092:1614-1633 Msa1465250:intergenic 45.0%
TGGCGCATAACTACTCAACA+GGG + tig0045092:1882-1901 Msa1465250:intergenic 45.0%
ATGGCGCATAACTACTCAAC+AGG + tig0045092:1883-1902 Msa1465250:intergenic 45.0%
TGCAATAAACCCCCTGTTCT+AGG - tig0045092:426-445 Msa1465250:three_prime_UTR 45.0%
TGCCTCAAATGCCTAGAACA+GGG + tig0045092:440-459 Msa1465250:intergenic 45.0%
GTCGGTTGAGACATGTGAAA+AGG + tig0045092:486-505 Msa1465250:intergenic 45.0%
GACATCCCAATACATGTCTG+CGG - tig0045092:650-669 Msa1465250:CDS 45.0%
ACTGTGCTCTACTGTACAGT+TGG - tig0045092:723-742 Msa1465250:CDS 45.0%
AAACTTCAGCACACTCGTAC+GGG + tig0045092:750-769 Msa1465250:intergenic 45.0%
GCTGAAAGATGAACTTGCCA+AGG - tig0045092:886-905 Msa1465250:CDS 45.0%
ACAAAAGGAACCTCGCATGA+TGG - tig0045092:1032-1051 Msa1465250:intron 45.0%
! AACCTCGCATGATGGAACTT+AGG - tig0045092:1040-1059 Msa1465250:intron 45.0%
TTCCTAAGTTCCATCATGCG+AGG + tig0045092:1045-1064 Msa1465250:intergenic 45.0%
! TTCTTTGGAGAAGGGATGCA+GGG + tig0045092:1428-1447 Msa1465250:intergenic 45.0%
! ATTCTTTGGAGAAGGGATGC+AGG + tig0045092:1429-1448 Msa1465250:intergenic 45.0%
TCACTATTACGACGAAGGCT+AGG - tig0045092:1508-1527 Msa1465250:five_prime_UTR 45.0%
ATCTGTGAACCAGACAGATG+AGG - tig0045092:1563-1582 Msa1465250:five_prime_UTR 45.0%
CAAGGTCAATCTCTCTGTCA+AGG + tig0045092:1614-1633 Msa1465250:intergenic 45.0%
TGGCGCATAACTACTCAACA+GGG + tig0045092:1882-1901 Msa1465250:intergenic 45.0%
ATGGCGCATAACTACTCAAC+AGG + tig0045092:1883-1902 Msa1465250:intergenic 45.0%
CCTCAAATGCCTAGAACAGG+GGG + tig0045092:438-457 Msa1465250:intergenic 50.0%
GCCTCAAATGCCTAGAACAG+GGG + tig0045092:439-458 Msa1465250:intergenic 50.0%
GTGCCTCAAATGCCTAGAAC+AGG + tig0045092:441-460 Msa1465250:intergenic 50.0%
! TTCTAGGCATTTGAGGCACC+TGG - tig0045092:442-461 Msa1465250:three_prime_UTR 50.0%
TGCCAAGGAAAATAGGCAGC+TGG - tig0045092:901-920 Msa1465250:CDS 50.0%
! TGCCAGCTGCCTATTTTCCT+TGG + tig0045092:906-925 Msa1465250:intergenic 50.0%
AAGGAAAATAGGCAGCTGGC+AGG - tig0045092:905-924 Msa1465250:CDS 50.0%
! TCTGCTGACTGTGTGAATGC+AGG - tig0045092:1231-1250 Msa1465250:intron 50.0%
GTGTGAATGCAGGAGATCAC+TGG - tig0045092:1241-1260 Msa1465250:intron 50.0%
! TCTTTGGAGAAGGGATGCAG+GGG + tig0045092:1427-1446 Msa1465250:intergenic 50.0%
TCTCTCTGTCAAGGAGTTGC+TGG + tig0045092:1605-1624 Msa1465250:intergenic 50.0%
CCTCAAATGCCTAGAACAGG+GGG + tig0045092:438-457 Msa1465250:intergenic 50.0%
GCCTCAAATGCCTAGAACAG+GGG + tig0045092:439-458 Msa1465250:intergenic 50.0%
GTGCCTCAAATGCCTAGAAC+AGG + tig0045092:441-460 Msa1465250:intergenic 50.0%
! TTCTAGGCATTTGAGGCACC+TGG - tig0045092:442-461 Msa1465250:three_prime_UTR 50.0%
TGCCAAGGAAAATAGGCAGC+TGG - tig0045092:901-920 Msa1465250:CDS 50.0%
! TGCCAGCTGCCTATTTTCCT+TGG + tig0045092:906-925 Msa1465250:intergenic 50.0%
AAGGAAAATAGGCAGCTGGC+AGG - tig0045092:905-924 Msa1465250:CDS 50.0%
! TCTGCTGACTGTGTGAATGC+AGG - tig0045092:1231-1250 Msa1465250:intron 50.0%
GTGTGAATGCAGGAGATCAC+TGG - tig0045092:1241-1260 Msa1465250:intron 50.0%
! TCTTTGGAGAAGGGATGCAG+GGG + tig0045092:1427-1446 Msa1465250:intergenic 50.0%
TCTCTCTGTCAAGGAGTTGC+TGG + tig0045092:1605-1624 Msa1465250:intergenic 50.0%
! CCCCCTGTTCTAGGCATTTG+AGG - tig0045092:435-454 Msa1465250:three_prime_UTR 55.0%
! CCCCCTGTTCTAGGCATTTG+AGG - tig0045092:435-454 Msa1465250:three_prime_UTR 55.0%
! TGCAGGAGATCACTGGCTGC+AGG - tig0045092:1248-1267 Msa1465250:CDS 60.0%
! GCAGGAGATCACTGGCTGCA+GGG - tig0045092:1249-1268 Msa1465250:CDS 60.0%
! CAGGAGATCACTGGCTGCAG+GGG - tig0045092:1250-1269 Msa1465250:CDS 60.0%
! TGCAGGAGATCACTGGCTGC+AGG - tig0045092:1248-1267 Msa1465250:CDS 60.0%
! GCAGGAGATCACTGGCTGCA+GGG - tig0045092:1249-1268 Msa1465250:CDS 60.0%
! CAGGAGATCACTGGCTGCAG+GGG - tig0045092:1250-1269 Msa1465250:CDS 60.0%
Chromosome Type Strat End Strand Name
tig0045092 gene 296 2017 296 ID=Msa1465250;Name=Msa1465250
tig0045092 mRNA 296 2017 296 ID=Msa1465250-mRNA-1;Parent=Msa1465250;Name=Msa1465250-mRNA-1;_AED=0.05;_eAED=0.05;_QI=570|1|1|1|1|1|4|295|129
tig0045092 exon 296 751 296 ID=Msa1465250-mRNA-1:exon:24734;Parent=Msa1465250-mRNA-1
tig0045092 exon 860 980 860 ID=Msa1465250-mRNA-1:exon:24733;Parent=Msa1465250-mRNA-1
tig0045092 exon 1245 1291 1245 ID=Msa1465250-mRNA-1:exon:24732;Parent=Msa1465250-mRNA-1
tig0045092 exon 1387 2017 1387 ID=Msa1465250-mRNA-1:exon:24731;Parent=Msa1465250-mRNA-1
tig0045092 five_prime_UTR 1448 2017 1448 ID=Msa1465250-mRNA-1:five_prime_utr;Parent=Msa1465250-mRNA-1
tig0045092 CDS 1387 1447 1387 ID=Msa1465250-mRNA-1:cds;Parent=Msa1465250-mRNA-1
tig0045092 CDS 1245 1291 1245 ID=Msa1465250-mRNA-1:cds;Parent=Msa1465250-mRNA-1
tig0045092 CDS 860 980 860 ID=Msa1465250-mRNA-1:cds;Parent=Msa1465250-mRNA-1
tig0045092 CDS 591 751 591 ID=Msa1465250-mRNA-1:cds;Parent=Msa1465250-mRNA-1
tig0045092 three_prime_UTR 296 590 296 ID=Msa1465250-mRNA-1:three_prime_utr;Parent=Msa1465250-mRNA-1
Gene Sequence

>Msa1465250

ATGGCCTATGGATATGATCAGCTGAAAGATGAACTTGCCAAGGAAAATAGGCAGCTGGCAGAGGAAAATCTACAAAAGGAACCTCGCATGATGGAACTTAGGAATCAGTGTAGAATAATTCGTACAACCGAATTAGCTACTGCTAATGAGAAACTAAACGAGCTTGAGAAGCAAAAAGAAGAAATGTTAAAGATGAATTCCCCTGCATCCCTTCTCCAAAGAATTCAAGAATCTGTGAACCAGACAGATGAGGAATCTGAAAATCTGCACCAGCAACTCCTTGACAGAGAGATTGACCTTGCAGCTTTTTTGCAGAAATACAAGAAGCTACGCACCACTTACCACAAGAGAACTCTTGTACATCTTGCTGCTAAAACATCAATTATATGA
Protein sequence

>Msa1465250

MAYGYDQLKDELAKENRQLAEENLQKEPRMMELRNQCRIIRTTELATANEKLNELEKQKEEMLKMNSPASLLQRIQESVNQTDEESENLHQQLLDREIDLAAFLQKYKKLRTTYHKRTLVHLAAKTSII*